Chapter 6.

Test selection

Chapter 6 Test selection

6.1 Sample setting

Here is described on sample setting in the Sample container.
There are two trays (Outer and Inner) in the Sample container.

A. Outer tray

Layer 1: position # 1 - 20
Layer 2: position # 21 – 40

Figure 6.1-1

B. Inner tray

Inner tray: position # 41 – 50

Figure 6.1-2

C. Sample tubes and cups

1. Sample tubes (in Outer tray):

Diameter: 12 – 16 mm
Length: 75 – 100 mm
Since this analyzer does not have piercing function, remove the rubber cap from
the sample tube in advance.

2. Sample cups (in Inner tray):

Normal size: 17mm“Ԅ”×38mm (2.0ml)

Pediatric size: 14mm“Ԅ”x 25mm (0.5ml)

z Set a sample cup for “Pediatric” on the specific sample tube (diameter: 13mm,
length: 85mm) and place it in the Outer tray.

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D. Sample positioning

Measurement starts in numeric order accordingly. The samples in the Inner tray are
measured prior to those in the outer tray. Tray selection varies depending on the
sample type.
When performing calibration at the same round, assign younger position # for the
calibrators than the position # for the normal samples.

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6.2 Test selection

Run(F5) > Test Selection

Perform calibrator registration for normal sample, emergency sample, or calibration.
Specify the location of the ISE wash solution and wash solution for the sample pipette
wash on this screen.

Figure 6.2-1

z When using other barcode reader type,

the keyboard connector type or handy type (keyboard wedges) is available to
enter the sample ID in the [Test Selection] screen. When applying your
handy barcode reader, tick the “Sample Barcode” ON.

1. Start
Specify the start position # in the Sample tray.
Select the position # (1 to 50) from drop-down menu.
Or specify the position directly from SID list.
For the samples with Barcode labels, do not specify position # and leave as
blank.
The position # 41 to 50 is not available for normal and emergency samples.

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2. End
Specify the end position # to copy or delete. (This is effective only when ticking
on “C/D”.)

z Sample Barcode Reader (option)

When applying barcode reader for Sample ID, no position assignment is
required.
Depending on the Sample type, reading barcode labels from barcode reader
allows to register and measure automatically.
For details, refer to the method list for each Sample type.

3. Sample Category
Specify the Sample Category from drop-down menu.
Sample Category Description

Normal Order registration for normal sample

STAT Order registration for emergency sample

Calibrator Order registration for calibrator

Multi-Calibrator Order registration for multi-calibrator

Diluted Calibration Order registration for diluted calibrator

QC Sample Order registration for QC Sample

Blank Order registration for blank sample

Mask Order registration for mask sample

Orderless Order registration for orderless sample

Wash Solution Order registration for Sample pipette wash solution

ISE Calibrator Order registration for ISE Calibrator

ISE Cleaning Order registration for ISE cleaning solution

ISE Etching Order registration for Etching

ISE Activation Order registration for ISE Activator

4. Template
Select the Template from drop-down menu.

5. Cup
Select “Normal” or “Pediatric” from drop-down menu.

6. Sample Barcode
Tick the “Sample Barcode” ON when applying barcode reader to enter the
sample ID.

7. C/D
Tick “C/D” ON when copying or deleting the registered order.

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8. E
Tick “E” ON when specifying normal sample as an emergency sample.

9. SID
Enter the Sample ID.

10. Sample Type

Select the Sample Type (Serum, Urine, Plasma, or Others) from drop-down
menu.

11. Normal Range

Select the Normal Range from drop-down menu.

12. PID
Enter the Patient ID. For the registered PID, select from drop-down menu.
When entering a new Patient ID, a registration screen for the PID appears.

13. Collection Date

Enter blood collection date.

14. Physician
Select Physician name from drop-down menu if any registered information.

15. Department
Select the Department from drop-down menu if any registered information.

16. Sample Comment

Select the Sample comment from drop-down menu if any registered information.

17. Number
Specify measurement number for replicate measurement. (This is effective for
normal sample and emergency sample.)

18. Sample Volume

Select the Sample volume (Low, Normal, or High) for initial measurement from
drop-down menu. For the figures for each volume, specify in the [Chemistry
Parameters1] screen.

19. SID List

Indicates information on the sample ID and its category, etc.

20. Restart
Perform to restart measurement. (Effective only when adding a sample.)

21. Rotate Sample Container

It gives the Sample tray rotation. (Effective only when adding a sample.)

22. Acquire orders

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It receives the order information from Host computer.

It is effective in “On Line Batch” or “On Line Real time” mode.

23. Save
Saves the setting.

24. Cancel
Cancels the editing data.

25. Print
Printouts the registered orders.

26. Delete
Deletes the “Pos” information you select.

27. Delete All

Deletes all of “Pos” information you registered.

28. Method display

This area indicates the method status for normal and ISE measurement in color
description.
The highest warning (in RED) will be displayed if more than two warnings are
applied.
Priority Background Warning description

High Red Calibration invalid or required.

Low Yellow QC measurement invalid or required.

- Gray The method is masked, or will not be run.

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6.2.1 Normal sample (N)

Measurement starts in “Pos” numerical order.
Inner tray is not available for normal samples.

Figure 6.2-2

A. Order setting procedure (with sample barcode reader)

1. Select "blank” on the Start# from drop-down menu.

2. Enter the Sample ID.

3. Specify the methods.

Tick the method name ON to specify.

4. Click the “Save” icon to confirm the order.

B. Order setting procedure (without sample barcode reader)

1. Select the Start# from drop-down menu for a sample.

2. Select the sample cup (“Normal” or “Pediatric”) from drop-down menu.

3. Select “Normal” in the Sample category from drop-down menu.

4. To preferentially order a normal sample as Emergency, tick ON at “E” when

adding the sample on the tray.

5. Enter “Sample ID”.

Available entry range: 3 to 15 digits
In the default setting, the assigned Pos. # automatically becomes the Sample
ID.

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z Not available to assign Sample ID for 89990000 – 99999999 (other category

IDs).

z When selecting “Pediatric” for sample cup, the Sample ID is assigned the
number with 8999 as first 4 digits.
When entering last 4 digits of the Sample ID, the first 4 digits will be added
automatically.

6. Select the Sample type (Serum, Urine, Plasma, or Others) from drop-down
menu.

7. Select the Sample volume (Low, Normal, or High) from drop-down menu.
For each volume of the sample, specify on the [Chemistry Parameters 1]
screen.

8. Select the Normal range from drop-down menu.

In the default setting, it is “Human Auto”.
Normal range is automatically selected from patient information such as age
and gender on PID registration screen and applied for judgment.
When not specifying in age of the patient, “G2” is applied. When not specifying
in gender, “Male“ is applied.

9. Select the Patient ID.

For the registered PID, select from drop-down menu.
When entering new PID, the registration screen appears. Register all
necessary items for the PID.

10. Specify blood collection date.

In the default setting, collection date will be the entry date, however, it can be
modified by entering directly or clicking on the calendar from drop-down menu.

11. Enter the Physician, the Department, and the Sample Comment.
Directly enter or select from the list of the drop-down menu.

12. Specify the Number (number of measurement) for the selected method from
drop-down menu.
In the default setting, it is "01". The available range is “1 – 99”.
When selecting “2” or more at “No.”, auto rerun is not performed.

13. Specify the method.

When ticking ON at the method name, its measurement will be ordered.
Check boxes for ISE measurement and profile (set of Methods) are located in
the lower part of the screen.

14. Click the “Save” icon to confirm the order.

The sample ID on the Pos.# appears in the order list on the left side of the
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screen after confirming the order.

C. Copy procedure

To copy the same order to several sample orders,

1. Select the Pos.# to be copied.

2. Tick “C/D" ON.

3. Select the Start # as the beginning Position # for copying from drop-down menu.

4. Select the End # as the end Position # for copying from drop-down menu.

5. Click the “Save” icon to confirm the order.

D. Delete procedure

1. Click the “Delete All” icon to delete all orders.

2. When deleting the specific order, select the Pos” or SID on the SID list and click
the “Delete” icon.

3. When deleting a series of SID, tick “C/D” ON.

Specify the Start # and the End # and click the “Delete” icon.

4. Click the “Save” icon to confirm the order.

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6.2.2 Emergency sample addition (STAT)

Measurement starts in “Pos” numerical order. When adding an emergency sample
during measurement in process, the emergency measurement is given priority over the
normal samples.
Inner tray is not available for emergency samples.

Figure 6.2-3

A. Order setting procedure (with sample barcode reader)

1. Enter the Sample ID.

2. Specify the methods.

Tick the method name ON to specify.

3. Click the “Save” icon to confirm the order.

B. Order setting procedure (without sample barcode reader)

1. Select the Start# from drop-down menu.

2. Select “STAT” in the Sample category from drop-down menu.

3. Select the sample cup.

Select the cup type (“Normal” or “pediatric”) from drop-down menu.

4. Specify the Sample ID.

When selecting “Normal” for sample cup, enter or specify SID # (001 to 899) in 3
digits.
When selecting “Pediatric” for sample cup, enter or specify SID # (01 to 99) in 2
digits.

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z The SID# for Emergency sample is started with “99000” in 8 digits. The SID#
is classified by sample cup type.

z When selecting "Normal” as sample cup, “99000” as the first 5 digits is added
in the SID.

z When selecting "Pediatric” as sample cup, “990009” as the first 6 digits is

added in the SID.

5. Enter “Patient ID”.

For registration, refer to “6.2.1. Normal sample (N)”.

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6.2.3 QC sample (Q)

Perform QC sample measurement and save as the QC results.
Measurement starts in “Pos” numerical order
Both Outer and inner trays are available for setting QC samples.
When setting the QC samples in Inner tray, they are measured prior to those in Outer
tray.

Figure 6.2-4

A. Order setting procedure (with sample barcode reader)

Under managing with barcode reader, the analyzer recognizes the necessary
information from barcode labels.
No order registration is required except placing necessary QC samples in the Sample
container.

B. Order setting/Delete procedure (without sample barcode reader)

For the QC samples set in Inner tray or without managing with barcode reader, follow
the procedure below.

1. Select the Start# from drop-down menu.

2. Select “QC sample” in the Sample category from drop-down menu.

3. Select the QC from drop-down menu.

4. The registered method items are automatically selected.

5. Click the “Save” icon to confirm the order. The SID for the QC sample is listed
on the SID list.

6. For deleting, click the SID on the SID list, click “Delete” icon.
For the deletion confirmation, click the “Save” icon.

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6.3 Test selection (other sample categories)

6.3.1 Calibrator (C)
Perform calibration using calibrators and prepare calibration curves.
Measurement starts in “Pos” numerical order.
Calibrator is available to set in both Inner and Outer trays.
The calibrators in Inner tray will be measured first.

Figure 6.3-1

A. Order setting procedure (with sample barcode reader)

Under managing with barcode reader, the analyzer recognizes the necessary
information from barcode labels.
No order registration is required except placing necessary QC samples in the Sample
container.

B. Order setting/Delete procedure (without sample barcode reader)

For the calibrators set in Inner tray or without managing with barcode reader, follow the
procedure below.

1. Specify the Pos.# (1 – 50) for calibrator from drop-down menu.

2. Select “Calibrator” in the Sample category from drop-down menu.

3. Select the Sample type (Serum, Urine, Plasma, or Others) from drop-down
menu.

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4. Specify the method. When ticking ON at the method name, its measurement
will be ordered.
(This is available to select only 1 method. When using a single calibrator to
prepare several calibration curves for the methods, select “Multi-Calibrator.”)
The method that registered on the [Calibration Registration] screen is available
to be ordered.

5. Click the “Save” icon to confirm the order.

6. For deleting, click the SID on the SID list, click the “Delete” icon.
For the deletion confirmation, click the “Save” icon.

6.3.2 Multi-Calibrator
Using a single calibrator, prepare several calibration curves for the methods.
Measurement starts in “Pos” numerical order.
Multi-Calibrators can be set in both Inner and Outer trays. The multi-calibrators in the
Inner tray will be measured first.

Figure 6.3-2

A. Order setting procedure (with sample barcode reader)

Under managing with barcode reader, the analyzer recognizes the necessary
information from barcode labels.
No order registration is required except placing necessary multi-calibrators in the
Sample container.

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B. Order setting/Delete procedure (without sample barcode reader)

For the multi-calibrators set in Inner tray or without managing with barcode reader,
follow the procedure below.

1. Specify the Pos.# (1 – 50) for multi-calibrator from drop-down menu.

2. Select “Multi-Calibrator” in the Sample category from drop-down menu.

3. Select the Sample type (Serum, Urine, Plasma, or Others) from drop-down
menu.

4. Select a multi-calibrator name on “MS” from drop-down menu.

5. The registered method items are ordered.

6. Select number of measurement on “No.” from drop-down menu. When

performing regular calibration, select "Full” on “No.” from drop-down menu.
When performing recalibration, select the calibrator # (C1 to C7) for calibrator
on the SID list.

7. Click the “Save” icon to confirm the order. The SID for the Multi-Calibrator
sample is listed on the SID list.

8. For deleting, click the SID on the SID list, click the “Delete” icon.
For the deletion confirmation, click the “Save” icon.

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6.3.3 Diluted calibration

Perform measurements with automatic dilution of a calibrator several times and
prepare a multi-point calibration curve.
Prior to the measurement, conditions for diluting are required to be set on the [Diluted
Calibration] screen.
Diluted Calibrators are available to set in both Inner and Outer trays.
The Diluted Calibrator in the Inner tray will be measured first.

Figure 6.3-3

A. Order setting procedure (with sample barcode reader)

Under managing with barcode reader, the analyzer recognizes the necessary
information from barcode labels.
No order registration is required except placing necessary diluted calibration samples
in the Sample container.

B. Order setting/Delete procedure (without sample barcode reader)

For the diluted calibrators set in Inner tray or without managing with barcode reader,
follow the procedure below.

1. Specify the Start# for the Diluted Calibrator from drop-down menu.

2. Select “Diluted calibration” in the Sample category from drop-down menu.

3. Select the Sample type (Serum, Urine, Plasma, or Others) from drop-down
menu.

4. Specify the method. Tick the method ON for the measurement order.

5. Click the “Save” icon to confirm the order. The SID for the Diluted calibration
sample is listed on the SID list.

6. For deleting, click the SID on the SID list, click the “Delete” icon.
For the deletion confirmation, click the “Save” icon.

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6.3.4 Blank sample

Measure the blank sample (C1: no-concentration calibrator) for recalibration. Prepare
a calibration curve.
Measurement starts in “Pos” numerical order. Blank sample is available to set in both
Inner and Outer trays. The blank samples in Inner tray will be measured first.

Figure 6.3-4

A. Order setting procedure (with sample barcode reader)

Under managing with barcode reader, the analyzer recognizes the necessary
information from barcode labels.
No order registration is required except placing necessary blank samples in the
Sample container.

B. Order setting/Delete procedure (without sample barcode reader)

For the blank samples set in Inner tray or without managing with barcode reader, follow
the procedure below.

1. Specify the Start# for a blank sample from drop-down menu.

2. Select “Blank” in the Sample category from drop-down menu.

3. Select the Sample type (Serum, Urine, Plasma, or Others) from drop-down
menu.

4. All of the methods registered on the [Parameter(F6)] > [Chemistry Parameters

2] screen are ordered to correct a calibration curve using C1 Blank.

5. Click the “Save” icon to confirm the order. The SID for the blank sample is
listed on the SID list.

6. For deleting, click the SID on the SID list, click the “Delete” icon.
For the deletion confirmation, click the “Save” icon.

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6.3.5 Mask
The Mask setting allows to set methods not performed. The methods indicate in a
measureable status that has analysis conditions, the calibration curves, and its
reagents in the Reagent container.

Figure 6.3-5

A. Mask selection/release

1. Select “Mask” in the Sample category from drop-down menu.

2. Select the Mask type (Normal, QC sample, or Calibrator) from drop-down menu.

3. The mask status on the methods is displayed.

4. By ticking the method ON to unselect the mask, the method becomes unmasked
and measurable status. Tick the checkmark again to remove the checkmark.
It becomes the mask status active.

5. To make all methods masked, click the [Mask All] button.

After clicking this button, it is changed into the [Clear All] button.

6. To make all methods unmasked, click the [Clear All] button.

7. Click the “Save” icon to confirm the order.

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6.3.6 Orderless
Orderless selection allows to perform measurement in all methods available for all
samples in the Sample container.
Prior to orderless measurement, specify the methods to be measured.
For the orderless measurement, refer to “7.1.5 Orderless measurement.”

Figure 6.3-6

A. Setting procedure for orderless measurement

1. Select “Orderless” in the Sample category from drop-down menu.

2. Select the Sample type (Serum, Urine, Plasma, or Others) from drop-down
menu.

3. Select the Normal range from the pop-up list by clicking the drop-down menu.
It is “Human Auto” as the default setting.
Normal range is automatically selected from patient information such as age
and gender registered in the [Patient Information] screen and applied for
judgment.
When not specifying in age of the patient, “G2” is applied. When not specifying
in gender, “Male” is applied.

4. Specify the Number (number of measurement) for the selected method from
drop-down menu.
It is "01" as the default setting. The available range is “1 – 99”.
When selecting “2” or more, auto rerun is not performed.

5. Specify the methods to be measured or not.

When ticking ON at the method name, its measurement will be ordered.

z By clicking the check box, it switches between ON and OFF.

6. Click the “Save” icon to confirm the order.

z When some orders specified in the [Test Selection] screen, the orders are
measured prior to the orderless measurement.
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6.3.7 Sample pipette wash

The wash solution for cleaning the sample pipette needs to be set in the Sample
container.
With the sample barcode reader, the position of the wash solution is confirmed by
reading the barcode label.
Without the sample barcode reader, the wash solution position needs to be specified.
Apply Wash solution C-1 solution by making a 1% Wash solution C-1 solution.
For the sample pipette washing, refer to the [Run(F5)] > [Daily Maintenance] screen.

Figure 6.3-7

A. Setting for wash solution

1. Select the Start# from drop-down menu for the wash solution.

2. Select “Wash Solution” in the Sample category from drop-down menu.

3. Click the “Save” icon to confirm the order.

z SID list indicates the SID No. “96000201” as the Sample ID code.

B. Release for wash solution

1. Select the Start# from drop-down menu for the wash solution.

2. Click the “Delete” icon to delete the setting.

3. Click the “Save” icon to confirm the deletion.

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6.3.8 ISE calibrator (option)

For ISE calibration, ISE calibrator (H Solution) needs to be placed in the Sample
container and specified its position.
With the sample barcode reader, the position of the ISE calibrator can be read from the
barcode label.
Without the sample barcode reader, the position of the ISE calibrator needs to be
specified.
For the ISE calibration, refer to “5.2.1 Perform ISE calibration.”

z 500 µL of the H solution is required for one ISE calibration procedure.

Figure 6.3-8

A. Setting procedure for ISE calibrator

1. Select the Start# from drop-down menu for the ISE calibrator.

2. Select “ISE Calibrator” in the Sample category from drop-down menu.

3. Click the “Save” icon to confirm the order.

z SID list indicates the SID No. “96000001” as the Sample ID code.

B. Deletion procedure for ISE calibrator

1. Select the Start# from drop-down menu for the ISE calibrator.

2. Click the “Delete” icon to delete the setting.

3. Click the “Save” icon to confirm the deletion.

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6.3.9 ISE cleaning (option)

ISE cleaning requires ISE cleaning solution (CLEAN99K). The ISE cleaning solution
needs to be placed in the Sample container and specified its position.
With the sample barcode reader, the position of the ISE cleaning solution can be read
from the barcode label.
Without the sample barcode reader, the position of the ISE cleaning solution needs to
be specified.
For the ISE cleaning, refer to “18.1.1 ISE cleaning (option).”

z 500 µL of the ISE cleaning solution (CLEAN99K) is required for one ISE
cleaning procedure.

Figure 6.3-9

A. Setting for ISE cleaning solution

1. Select the Start# from drop-down menu for the ISE cleaning solution.

2. Select “ISE Cleaning” in the Sample category from drop-down menu.

3. Click the “Save” icon to confirm the order.

z SID list indicates the SID No. “96000201” as the Sample ID code.

B. Deletion procedure for the ISE cleaning solution

1. Select the Start# from drop-down menu for the ISE cleaning solution.

2. Click the “Delete” icon to delete the setting.

3. Click the “Save” icon to confirm the deletion.

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6.3.10 ISE Etching (option)

Cleaning of the Na electrode requires ISE Etching. The ISE Etching needs to be
placed on the Sample container and specified its position.
With the sample barcode reader, the position of the ISE Etching can be read from the
barcode label.
Without the sample barcode reader, the position of the ISE Etching needs to be
confirmed.
For the ISE Etching, refer to “18.2.5 ISE electrodes (option).”

z 500 µL of the ISE Etching is required for one ISE cleaning 2 procedure.

Figure 6.3-10

A. Setting procedure for the ISE Etching

1. Select the Start# from drop-down menu for the ISE Etching.

2. Select “ISE Etching” in the Sample category from drop-down menu.

3. Click the “Save” icon to confirm the order.

z SID list indicates the SID No. “96000301” as the Sample ID code.

B. Deletion procedure for the ISE Etching

1. Select the Start# from drop-down menu for the ISE Etching.

2. Click the “Delete” icon to delete the setting.

3. Click “Save” icon to confirm the deletion.

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6.3.11 ISE activation (option)

Perform ISE electrodes activation for stable results after replacement. The ISE
activator (serum or specific QC sample) needs to be placed on the Sample container
and specified its position.
With the sample barcode reader, the position of the ISE activator can be read from the
barcode label.
Without the sample barcode reader, the position of the ISE activator needs to be
specified.
For the ISE activation, refer to “C. Procedure on the ISE etching” of “18.2.5 ISE
electrodes (option).”

z 500 µL of the ISE activator is required for one ISE activation procedure.

Figure 6.3-11

A. Setting procedure for the ISE activation

1. Select the Start# from drop-down menu for the ISE activator.

2. Select “ISE Activation” in the Sample category from drop-down menu.

3. Click the “Save” icon to confirm the order.

z SID list indicates the SID No. “96000401” as the Sample ID code.

B. Deletion procedure for the ISE activation

1. Select the Start# from drop-down menu for the ISE activator.

2. Click the “Delete” icon to delete the setting.

3. Click the “Save” icon to confirm the order.

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6.4 Template
It is available to select the Template that set the samples in the sample tray with its
specified methods as a set order.
The template is required to register in the [Auto Template] screen prior to selecting in
the [Test Selection] screen. For registering the template setting, refer to “14.8
Template setting.”

Figure 6.4-1

1. Select the template # in the Template from drop-down menu.

2. After selecting the template #, the following pop-up message appears to

overwrite the order information of the registered screen. To overwrite the
information, click the [OK] button.

Figure 6.4-2

3. Click the “Save” icon to confirm the order.

z The template function is not available for the normal samples.

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6.5 Test selection (online)

6.5.1 Online sample selection
For online test order, host communication is required with either “Online real time” or
“Online batch mode”.

<When applying the Sample barcode reader>

When selecting “On line Real time” at the Host Communication Mode on the [System
Setup 1] screen, automatically receives orders from host connection and no need the
order setting.
When selecting “On Line Batch” at the Host Communication Mode on the [System
Setup 1] screen, it needs to obtain orders from host communication.

<When not applying the Sample barcode reader>

When selecting “On Line Real time” at the Host Communication Mode on the [System
Setup 1] screen, it needs to register the position # and the SID to specify the position #
manually placed on the sample tray.
When selecting “On Line Batch” at the Host Communication Mode on the [System
Setup 1] screen, it needs to register the position # and the SID to specify the position #
manually placed on the sample tray. It also needs to obtain orders from host
communication.

A. Order setting procedure (in the “On Line Batch mode” with the Sample barcode
reader)

1. Select "blank” on the Pos. # from drop-down menu.

2. Enter the Sample ID.

3. Click the [Acquire orders] button.

The order information including the methods can be downloaded from host
connection.

4. Click the “Save” icon to confirm the order.

B. Order setting procedure (in the On Line Real time mode without the Sample
barcode reader)

1. Select the Pos. # from drop-down menu.

2. Enter “Sample ID”.

3. Click the “Save” icon to confirm the order.

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C. Order setting procedure (in the On Line Batch mode without the Sample
barcode reader)

1. Select the Pos. # from drop-down menu.

2. Enter “Sample ID”.

3. Click the [Acquire orders] button.

The order information including the methods can be downloaded from host
connection.

4. Click the “Save” icon to confirm the order.

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