UNIVERSITY OF JAMMU

SYLLABI AND COURSE OF STUDY IN

BIOTECHNOLOGY
For the Examination to be held in Year 2022,
2023, 2024 &2025

BIOTECHNOLOGY COURSE

UG SEMESTER I & II
UNDER NEP-2020
 UNIVERSITY OF JAMMU
SYLLABI AND COURSES OF STUDY IN BIOTECHNOLOGY
For the examination to be held in December 2022, 2023, 2024
UG SEMESTER-I
UNDER NEP-2020

S. Course type Course Course Title Credits Marks Total

No No. Theory Practical/Tutorial Marks

1. Major UMJBTT- Fundamentals of 4 (3+1) Mid End Assessment Exam: 100

101 Biotechnology -I Semester: exam: 10 marks 15
15 Marks 60 Marks
marks
2. Minor UMIBTT- Introduction to 4 (3+1) Mid End Assessment Exam: 100
102 Biotechnology -I Semester: exam: 10 marks 15
15 Marks 60 Marks
marks
3. Multi- UMDBTT- Biotechnology for 3+0 Mid End NA NA 75
disciplinary Semester: Semester
103 Human Welfare
15 Marks Exam:
60
Marks
4. Skill USEBTT- Bioinstrumentation 2 (40 Mid End NA Exam: 50
Enhancement marks Semester: Semester 10
104 and Analytical
theory +1 10 Marks Exam: Marks
techniques marks 30
practicals) Marks
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – I
(Examination to be held in December 2022, 2023, 2024)
MAJOR COURSE

Course Code: UMJBTT-101

Course Title: Fundamentals of Biotechnology-I
Credits: 4 (3Theory+1Practical)
Total No. of Lectures: Theory: 45 hours
Practical: 30 hours
Maximum Marks: 100
Theory: 75
Practical: 25
Duration of Examination: 3 hours

Objectives and Expected Learning Outcomes

The course provides an introduction to the fundamentals of Biotechnology with the major focus to
cell biology, genetics, biomolecules, microbiology and molecular biology. After successfully
completing this course, the students will be able to understand the basic concepts in cell biology,
genetics, molecular biology and microbiology; understand the structure and biological significance
of biomolecules.

Unit 1: Cell Biology and Genetics

Cell theory, Cell as basic unit of life (Viral, bacterial, fungal, plant and animal cells), Ultra structure
of prokaryotic and eukaryotic cells (Cell wall, cell membrane, Golgi Complexes, Endoplasmic
Reticulum, Peroxisome, Lysosomes etc), Semi- autonomous Organelles (Mitochondria &
Chloroplast: Endosymbiotic theory), Nucleus: Nuclear envelope with nuclear pore complex,
Nucleolus, Nucleoplasm and Chromatin
Historical overview; Understanding Mendel’s principles, symbols and terminologies, Laws of
dominance, segregation and independent assortment; Gene interactions and their outcome

Unit 2: Introduction to Biomolecules

Carbohydrates: Definition, functions and classification of carbohydrates. Oligosaccharides,
Polysaccharides-components and functions. Amino acids: Structures and Classifications Proteins:
Physical properties: salting in and salting out, peptide bond, Organization of protein structure into
primary, secondary, tertiary and quaternary structures. Lipids: Introduction, classification, properties
of Fatty acids, Saturated and unsaturated fatty acids, Essential fatty acids, Triacylglycerol, simple
and mixed Triacylglycerol. Nucleotides: Introduction, purine and pyrimidine bases, General
composition of nucleic acids, nucleosides, nucleotides.

Unit 3: Microbiology
History and development of Microbiology; Microscopy: Principle and applications of different
types of microscopes; Prokaryotes: diversity and taxonomy, cell structure and function; Methods in
microbiology (sterilization, culturing methods, staining techniques); Microbiological media,
composition and types; Applications of Microbiology.

Unit 4: Molecular Biology

DNA and RNA as a genetic material, DNA and RNA Structure and components, Central Dogma of
Molecular Biology, General features of DNA replication and basic rules of replication. Basic
concept of transcription and translation in prokaryotes and eukaryotes.
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – I
(Examination to be held in December 2022, 2023, 2024)
MAJOR COURSE

Course Code: UMJBTT-101

Course Title: Fundamentals of Biotechnology-I
Credits: 4 (3Theory+1Practical)
Total No. of Lectures: Theory: 45 hours
Practical: 30 hours
Maximum Marks: 100
Theory: 75
Practical: 25
Duration of Examination: 3 hours

Practical
1. Study, use and maintenance compound microscope
2. To study different types of plant cells and animal cells.
3. Qualitative test for detection of carbohydrates in the solution.
4. Qualitative test for detection of lipids in the solution.
5. Qualitative test for detection of proteins in the solution.
6. Preparation of culture media, isolation of microorganisms from soil, air and water.
7. Colony purification, bacterial staining: simple staining, negative staining and Gram’s
staining
8. Problems based on monohybrid, dihybrid, trihybrid crosses
9. Problems based on chi square test.
10. Isolation of genomic DNA from living cell and quantification of DNA by Spectrophotometer.

NOTE FOR PAPER SETTING

Examination Theory / Syllabus to be covered Time Allotted for % Weightage (Marks)

Practical in the Examination Exam
Mid Term Assessment 50% 1 ½ Hours 15
test
External Theory End 100% 3 Hours 60
Semester
Internal Practical - - 10
(Based on Daily
Performance only)
External Practical - - 15
A) Mid Term Assessment test: (15 Marks) Time Allotted 1 ½ Hours

B) External End Semester Examination: (60 Marks) Time Allotted 3 Hours

a) External End Semester Theory Examination will have two sections (A & B).
b) Section A shall be of 12 Marks and will comprise of 4 short answer type questions one
question from each unit carrying 03 Marks each. A candidate will have to attempt all the
questions.
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – I
(Examination to be held in December 2022, 2023, 2024)
MAJOR COURSE

Course Code: UMJBTT-101

Course Title: Fundamentals of Biotechnology-I
Credits: 4 (3Theory+1Practical)
Total No. of Lectures: Theory: 45 hours
Practical: 30 hours
Maximum Marks: 100
Theory: 75
Practical: 25
Duration of Examination: 3 hours

c) Section B shall be of 48 Marks and will comprise of 8 long answer type questions, two from
each unit. A candidate will have to attempt four questions selecting one question from each
unit. Each question will carry 12 marks.

Reference books
1. Cell and Molecular Biology Concepts and Experiments- G. Karp, John Wiley & Sons, Inc.,
8th edition (2015).
2. Fundamentals of Biochemistry- Jain and Jain; S. Chand., 7th edition (2016).
3. Cell Biology- D.E. Robertis& De Roberis, Blaze publishers & Distributors Pvt. Ltd., 8th
edition (2017).
4. The Cell: A Molecular Approach- Cooper, G.M. and Hausman, R.E., Oxford University
Press, Washington, D.C.; Sinauer Associates, MA., 8th edition (2019).
5. Basic Genetics- Daniel L. Hartl, Jones &Barlett Publishers USA, latest edition.
6. Genetics – Monroe W Strickberger, Macmillain Publishers, New York, latest edition.
7. Principles of Gene Manipulations- Old & Primrose, Black Well Scientific Publications, 8th
edition (2016).
8. Lewin’s Genes XII - Elliott S. Goldstein, Jocelyn E. Krebbs, and Stephen T. Kilpatrick,
Jones and Bartlett Publishers, Inc., 12th edition (2018).
9. Microbiology- Prescott, L.M., Harley, J.P. and Klein, D.A. McGraw Hill, USA, 12th edition
(2022).
10. Microbiology-Pelczar, M.J.J., Chan, E.C.S. and Kreig, N.R. Tata McGraw Hill,New Delhi
(2005).
11. Microbiology: An Introduction-Tortora G.J.,Funke B.R. and Case C.L., Wever and Bair.
Pearson Education India, 13thedition (2021).
12. Lehninger's Principles of Biochemistry- Nelson and Cox, W. H., Freeman and company,
New York, 8th edition (2021).
13. Biochemistry- Voet D and VoetJG , John Wiley & Sons, New York, 6th edition (2004).
Biochemistry- Berg JM, Tymoczko, JL, and Stryer L. WH Freeman & Co., New York,9th
edition (2019).
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – I
(Examination to be held in December 2022, 2023, 2024)
MINOR COURSE

Course Code: UMIBTT-102

Course Title: Introduction to Biotechnology -I
Credits: 4 (3Theory+1Practical)
Total No. of Lectures: Theory: 45 hours
Practical: 30 hours
Maximum Marks: 100
Theory: 75
Practical: 25
Duration of Examination: 3 hours

Objectives and Expected Learning Outcomes

The course provides an introduction to the fundamentals of Biotechnology with the major focus to
cell biology, genetics, biomolecules, microbiology and molecular biology. After successfully
completing this course, the students will be able to understand the basic concepts in cell biology,
genetics, molecular biology and microbiology; understand the structure and biological significance
of biomolecules.

Unit 1: Cell Biology and Genetics

Cell theory, Cell as basic unit of life (Viral, bacterial, fungal, plant and animal cells), Ultra structure
of prokaryotic and eukaryotic cells (Cell wall, cell membrane, Golgi Complexes, Endoplasmic
Reticulum, Peroxisome, Lysosomes etc), Semi- autonomous Organelles (Mitochondria &
Chloroplast: Endosymbiotic theory)
Historical overview and laws of Inheritance; Understanding Mendel’s principles and deviations;
Gene interactions and their outcome; Linkage and crossing over

Unit 2: Introduction to Biomolecules

Carbohydrates: Definition, functions and classification of carbohydrates. Oligosaccharides,
Polysaccharides-components and functions. Amino acids: Structures and Classifications Proteins:
Physical properties: salting in and salting out, peptide bond, Organization of protein structure into
primary, secondary, tertiary and quaternary structures. Lipids: Introduction, classification, properties
of Fatty acids, Saturated and unsaturated fatty acids, Essential fatty acids, Triacylglycerol, simple
and mixed Triacylglycerol. Nucleotides: Introduction, purine and pyrimidine bases, General
composition of nucleic acids, nucleosides, nucleotides.

Unit 3: Microbiology
History and development of Microbiology; Microscopy: Principle and applications of different
types of microscopes; Prokaryotes: diversity and taxonomy, cell structure and function; Methods in
microbiology (sterilization, culturing methods, staining techniques); Microbiological media,
composition and types; Applications of Microbiology.

Unit 4: Molecular Biology

DNA and RNA as a genetic material, DNA and RNA Structure and components, Central Dogma of
Molecular Biology, General features of DNA replication and basic rules of replication. Basic
concept of transcription and translation in prokaryotes and eukaryotes.
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – I
(Examination to be held in December 2022, 2023, 2024)
MINOR COURSE

Course Code: UMIBTT-102

Course Title: Introduction to Biotechnology -I
Credits: 4 (3Theory+1Practical)
Total No. of Lectures: Theory: 45 hours
Practical: 30 hours
Maximum Marks: 100
Theory: 75
Practical: 25
Duration of Examination: 3 hours

Practical
1. Study, use and maintenance compound microscope
2. To study different types of plant cells and animal cells.
3. Qualitative test for detection of carbohyderates in the solution.
4. Qualitative test for detection of lipids in the solution.
5. Qualitative test for detection of proteins in the solution.
6. Preparation of culture media, isolation of microorganisms from soil, air and water.
7. Colony purification, bacterial staining: simple staining, Negative staining and Gram’s
staining
8. Experiments on monohybrid, dihybrid, trihybrid cross
9. Experiments on chi square test.
10. Isolation of genomic DNA from living cell and quantification of DNA by Spectrophotometer.

NOTE FOR PAPER SETTING

Examination Theory / Syllabus to be covered Time Allotted for % Weightage (Marks)

Practical in the Examination Exam
Mid Term Assessment 50% 1 ½ Hours 15
test
External Theory End 100% 3 Hours 60
Semester
Internal Practical - - 10
(Based on Daily
Performance only)
External Practical - - 15

A) Mid Term Assessment test: (15 Marks) Time Allotted 1 ½ Hours

B) External End Semester Examination: (60 Marks) Time Allotted 3 Hours

a) External End Semester Theory Examination will have two sections (A & B).
b) Section B shall be of 48 Marks and will comprise of 8 long answer type questions, two from
each unit. A candidate will have to attempt four questions selecting one question from each
unit. Each question will carry 12 marks.
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – I
(Examination to be held in December 2022, 2023, 2024)
MINOR COURSE

Course Code: UMIBTT-102

Course Title: Introduction to Biotechnology -I
Credits: 4 (3Theory +1Practical)
Total No. of Lectures: Theory: 45 hours
Practical: 30 hours
Maximum Marks: 100
Theory: 75
Practical: 25
Duration of Examination: 3 hours

Reference books:

1. Cell and Molecular Biology: Concepts and Experiments- G. Karp, John Wiley & Sons, Inc.,
8th edition (2015).
2. Fundamentals of Biochemistry- Jain and Jain; S. Chand, 7th edition (2016).
3. Cell Biology- D.E. Robertis& De Roberis, Blaze publishers & Distributors Pvt. Ltd., 8th
edition (2017).
4. The Cell: A Molecular Approach- Cooper, G.M. and Hausman, R.E., Oxford University
Press, Washington, D.C.; Sinauer Associates, MA., 8th edition (2019).
5. Basic Genetics- Daniel L. Hartl, Jones &Barlett Publishers USA, latest edition.
6. Genetics – Monroe W Strickberger, Macmillain Publishers, New York, latest edition.
7. Principles of Gene Manipulations- Old & Primrose, Black Well Scientific Publications, 8th
edition (2016).
8. Lewin’s Genes XII - Elliott S. Goldstein, Jocelyn E. Krebbs, and Stephen T. Kilpatrick,
Jones and Bartlett Publishers, Inc., 12th edition (2018).
9. Microbiology- Prescott, L.M., Harley, J.P. and Klein, D.A. McGraw Hill, USA, 12th edition
(2022).
10. Microbiology- Pelczar, M.J.J., Chan, E.C.S. and Kreig, N.R. Tata McGraw Hill, New Delhi
(2005).
11. Microbiology: An Introduction- Tortora G.J., Funke B.R. and Case C.L., Wever and Bair.
Pearson Education India, 13th edition (2021).
12. Lehninger's Principles of Biochemistry- Nelson and Cox, W. H., Freeman and company,
New York, 8th edition (2021).
13. Biochemistry- Voet D and VoetJG , John Wiley & Sons, New York, 6th edition (2004).
Biochemistry- Berg JM, Tymoczko, JL, and Stryer L. WH Freeman & Co., New York, 9th
edition (2019).
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – I
(Examination to be held in December 2022, 2023, 2024)
MULTIDISCIPLINARY COURSE

Course Code: UMDBTT-103

Course Title: Biotechnology for Human Welfare
Credits: 3
Total No. of Lectures: 45 hours
Maximum Marks: 75
Theory: 75
Duration of Examination: 3 hours

Objectives and Expected Learning Outcomes: The course provides an introduction to

biotechnology and the application of biotechnology for human welfare including Agriculture,
industry, environment and human health care. After successfully completing this course, the
students will be able to understand the scope and application of biotechnology in various areas.

Unit 1: Introduction to Biotechnology

Definition & scope of Biotechnology; Introduction to DNA, RNA, Proteins and the Central Dogma;
Microbes and their Culturing; Principles of Genetic engineering & Bioprocess technology.

Unit 2: Applications of Biotechnology- Agriculture and Industry

Need for Genetically Modified (GM) crops, Golden rice, Bt Cotton &FlavrSavr Tomato;
Overview of Industrial production of alcoholic beverages, antibiotics & enzymes, Bio-fertilizers
and bio-pesticides, Bioplastics and Biofuels.

Unit 3: Biotechnology in Environment

Biodegradation of potential pollutants, recycling of wastes and other waste treatment technologies.
Controlling environmental pollution through bioremediation, biomonitoring, biotreatment and
biodegradation of all solid, liquid and gaseous wastes.

Unit 4: Biotechnology in Human healthcare

Human Genome Project, Gene therapy, Molecular Diagnostics tools - PCR, DNA fingerprinting, an
overview of vaccines, Recombinant insulin

NOTE FOR PAPER SETTING

Examination Theory / Syllabus to be Time Allotted for % Weightage (Marks)

Practical covered in the Exam
Examination
Internal Theory 50% 1½ Hours 15
Assessment
External Theory End 100% 3 Hours 60
Semester

A) Mid Term Assessment test: (15 Marks) Time Allotted 1 ½ Hours

 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – I
(Examination to be held in December 2022, 2023, 2024)
MULTIDISCIPLINARY COURSE

Course Code: UMDBTT-103

Course Title: Biotechnology for Human Welfare
Credits: 3
Total No. of Lectures: 45 hours
Maximum Marks: 75
Theory: 75
Duration of Examination: 3 hours

B) External End Semester Examination: (60 Marks) Time Allotted 3 Hours

a) External End Semester Theory Examination will have two sections (A & B).
b) Section A shall be of 12 Marks and will comprise of 4 short answer type questions one
question from each unit carrying 03 Marks each. A candidate will have to attempt all the
questions.
c) Section B shall be of 48 Marks and will comprise of 8 long answer type questions, two from
each unit. A candidate will have to attempt four questions selecting one question from each
unit. Each question will carry 12 marks.

Reference Books:

1. Gene Cloning and DNA Analysis: An Introduction- Brown T. A., Wiley Blackwell, 8th
edition (2020).
2. Principles of Gene Manipulation and Genomics- Primrose S.B., Wiley India,7th edition
(2014).
3. Biotechnology: A textbook of Industrial Microbiology- Crueger W and Crueger A., Panima
Publishing Co. New Delhi, 3rd edition (2017).
4. Environmental Biotechnology Concepts and Applications- Hans-Joachim J. and Winter, J.
(2004)
5. Elements of Biotechnology- Gupta, P.K.,Rastogi and Co., Merrut, India, 2ndedition (2010).
6. Introduction to Plant Biotechnology- Chawla, H. S.; C RC Press, 3rd edition (2020).
7. Text Book of Biotechnology- Das, H.K., Wiley India Pvt. Limited, 5th edition (2017).
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – I
(Examination to be held in December 2022, 2023, 2024)
SKILL ENHANCEMENT COURSE

Course Code: USEBTT-104

Course Title: Bioinstrumentation and Analytical Techniques
Credits: 2 (40 marks theory+ 10 marks practicals)
Total No. of Lectures: Theory: 25 hours
Practical: 30 hours
Maximum Marks: 50
Theory: 40
Practical: 10
Duration of Examination: 2.5 hours

Objectives and Expected Learning Outcomes: The course provides the basic principles and
functioning of various bioinstruments and analytic techniques used in biotechnology labs. After
successfully completing this course, the students will be able to handle various bioinstruments and
perform analytical techniques like chromatography, mass spectrometry and spectroscopy.

Unit 1: Basic analytical techniques-I

Principle and functioning of micropipetts; Preparation of buffers and reagents; Principle, working
and functions of Electronic balance, Autoclave, Biosafety hood, Microscope, Centrifuge, Agarose
gel electrophoresis, Polyacrylamide gel electrophoresis, Thermocycler, Real time PCR, Gel
documentation system.

Unit 2: Basic analytical techniques-II

Basic concepts in chromatography; Types of Chromatography (Paper chromatography, Thin-layer
chromatography Column chromatography, Ion- exchange chromatography, Gas chromatography
and HPLC), Mass spectrometry, Spectroscopy (UV-Vis).

Unit 3: Practical
1. Handling and operation of Microscope
2. To prepare the buffer at required pH.
3. To perform PCR.
4. Electrophoresis of DNA.
5. Demonstration and operation of Gel documentation system.
6. Spectrophotometer/colorimeter(Beer-Lambert’s law).
7. Spectroscopic estimation of DNA (UV).
8. Demonstration on operation of Centrifugation.
9. To separate and study plant pigmentsby paper chromatography.

NOTE FOR PAPER SETTING

Total marks of the USEBTT-101 are 50, 20% marks shall be reserved for internal assessment (10
Marks). 80% of the marks (40 marks) shall be reserved for external examination to be conducted by
the University/College.

Internal Assessment Test (10 Marks) Time Allotted 1 Hour

 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – I
(Examination to be held in December 2022, 2023, 2024)
SKILL ENHANCEMENT COURSE

Course Code: USEBTT-104

Course Title: Bioinstrumentation and Analytical Techniques
Credits: 2 (40 marks theory+ 10 marks practicals)
Total No. of Lectures: Theory: 25 hours
Practical: 30 hours
Maximum Marks: 50
Theory: 40
Practical: 10
Duration of Examination: 2.5 hours

External End Semester Examination 40 Marks (Time Allotted 2½ Hours)

1. External theory exam shall be of 30 marks and consists of 2 sections.
a. Section A shall be of 10 marks and comprise of 4 short answer type questions of 2½
marks each, from Unit I and II (All compulsory).
b. Section B shall be of 20 marks and will comprise of four long type questions of 10
marks each, two from Unit I and two from Unit II. A candidate has to attempt one from
each unit.
2. External Practical Exam shall be of 10 marks to be conducted by the college/ University
with practical exercise of 5 marks and Viva voce of 5 marks.
Note: During the submission of marks to University of Jammu final pattern will be 10 (internal) and
40 (external).

Reference books

1. Methods in biotechnology- Hong, S. B., Rashid, M. B., & Santiago-Vázquez, L. Z. John,

Wiley & Sons, 1st edition (2016).
2. Wilson and Walker's principles and techniques of biochemistry and molecular biology-
Wilson, K., Hofmann, A., Walker, J. M., &Clokie, S., Cambridge University Press, 8th
edition (2018).
3. Analytical techniques in biochemistry and molecular biology- Katoch, R., Springer Science
& Business Media (2011).
4. Basic Methods in Microscopy: Protocols and Concepts from Cells: A Laboratory Manual-
Goldman, R. D., & Spector, D. L., Eds Cold Spring Harbor Laboratory Press, 1st edition
(2006).
5. Fundamentals of bioanalytical techniques and instrumentation- Ghosal, S., &Avasthi, A. S.,
Phi Learning Pvt. Ltd, 2nd edition (2018).
 UNIVERSITY OF JAMMU
SYLLABI AND COURSES OF STUDY IN BIOTECHNOLOGY
For the examination to be held in May 2023, 2024, 2025
UG SEMESTER-II
UNDER NEP-2020

S. Course type Course Course Title Credits Marks Total

No No. Theory Practical/Tutorial Marks

1. Major UMJBTT- Fundamental of 4 (3+1) Mid End Assessment Exam: 100

201 Biotechnology - Semester: exam: 10 marks 15
II 15 Marks 60 Marks
marks
2. Minor UMIBTT- Introduction to 4 (3+1) Mid End Assessment Exam: 100
202 Biotechnology - Semester: exam: 10 marks 15
II 15 Marks 60 Marks
marks
3. Multi- UMDBTT- Application of 3+0 Mid End NA NA 75
disciplinary Semester: Semester
203 Biotechnology
15 Marks Exam:
in Agriculture 60
Marks
4. Skill USEBTT- Methods in 2 (40 Mid End NA Exam: 50
Enhancement marks Semester: Semester 10
204 Microbiology
theory +1 10 Marks Exam: Marks
and marks 30
practicals) Marks
Biochemistry
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – II
(Examination to be held in May 2023, 2024, 2025)
MAJOR COURSE

Course Code: UMJBTT-201

Course Title: Fundamentals of Biotechnology-II
Credits: 4 (3Theory+1Practical)
Total No. of Lectures: Theory: 45 hours
Practical: 30 hours
Maximum Marks: 100
Theory: 75
Practical: 25
Duration of Examination: 3 hours

Objectives and Expected Learning Outcomes:

The course provides an introduction to the fundamentals of Biotechnology with the major focus to
recombinant DNA technology, Immunology, Enzyme and Bioprocess technology; plant and animal
biotechnology. After successfully completing this course, the students will be able to understand the
basic concepts in rDNA technology and Bioprocess technology and also understand the process of
immune system and mechanism of enzyme action.

Unit 1: Recombinant DNA technology

Concept of recombinant DNA technology and its enzymes in gene cloning; Method of gene cloning
and tools and techniques involved, Applications of recombinant DNA technology in medicine,
agriculture, veterinary sciences and protein engineering.

Unit 2: Immunology
Brief history and overview of immune system, Innate and Adaptive immunity, Active and Passive
immunity, cells and Hematopoiesis, organs of immune system, Concept of Antigen and Antibody,
Immunoglobulin and their types, monoclonal antibody generation, MHC complex, Antigen-
antibody interactions and assays used for detection (RIA and ELISA).
Unit 3: Enzyme and Bioprocess Technology
Introduction to Enzymes, Nomenclature and classification of Enzymes, brief introduction to active
site. Cofactors, coenzyme, prosthetic groups, holoenzyme and apoenzyme, Active site hypothesis
(lock-and-key, induced fit, strain or transition-state stabilization); Enzyme activity units, Factors
affecting enzyme activity (Enzyme concentration, substrate concentration, pH, temperature and
reaction time). Introduction to Bioprocess Technology, Types of bioprocesses- batch, continuous,
fed batch, Microbial growth kinetics Design and formulation of Media for industrial bioprocesses,
Sterilization of media and air, Bioreactor and its types.

Unit 4: Plant and Animal Biotechnology

In vitro culture: approaches & methodologies - preparation steps for tissue culture; surface
sterilization of plant material; basic procedure for aseptic tissue transfer; incubation of culture;
media composition & Growth hormones; micro-culture of plants.
Animal tissue culture, history, requirements for animal cell culture, aseptic conditions, types of
media, Primary and secondary culture, subculture, cryopreservation.
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – II
(Examination to be held in May 2023, 2024, 2025)
MAJOR COURSE

Course Code: UMJBTT-201

Course Title: Fundamentals of Biotechnology-II
Credits: 4 (3Theory+1Practical)
Total No. of Lectures: Theory: 45 hours
Practical: 30 hours
Maximum Marks: 100
Theory: 75
Practical: 25
Duration of Examination: 3 hours

Practicals

1. PCR Amplification of gene of interest

2. Cloning of gene of interest in plasmid
3. Understanding the concept and maintenance of aseptic conditions for animal cell culture lab
4. Preparation of the media
5. Demonstration of various sterilization techniques used in laboratory.
6. Preparation of culture media for tissue culture, bacteria and fungi.
7. Demonstration of various steps of micropropagation
8. Effect of reaction time on the activity of enzyme.
9. Effect of substrate concentration on enzyme activity,
10. Calculation of kinetic parameters such as Km, Vmax.
11. Effect of temperature and pH on the activity of enzyme, and pH and thermostabilityanaslysis
12. To perform ABO blood typing
13. To perform differential leukocyte count

NOTE FOR PAPER SETTING

Examination Theory / Syllabus to be covered Time Allotted for % Weightage (Marks)

Practical in the Examination Exam
Mid Term Assessment 50% 1 ½ Hours 15
test
External Theory End 100% 3 Hours 60
Semester
Internal Practical - - 10
(Based on Daily
Performance only)
External Practical - - 15
A) Mid Term Assessment test: (15 Marks) Time Allotted 1 ½ Hours

B) External End Semester Examination: (60 Marks) Time Allotted 3 Hours

a) External End Semester Theory Examination will have two sections (A & B).
b) Section A shall be of 12 Marks and will comprise of 4 short answer type questions one
question from each unit carrying 03 Marks each. A candidate will have to attempt all the
questions.
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – II
(Examination to be held in May 2023, 2024, 2025)
MAJOR COURSE

Course Code: UMJBTT-201

Course Title: Fundamentals of Biotechnology-II
Credits: 4 (3Theory+1Practical)
Total No. of Lectures: Theory: 45 hours
Practical: 30 hours
Maximum Marks: 100
Theory: 75
Practical: 25
Duration of Examination: 3 hours

c) Section B shall be of 48 Marks and will comprise of 8 long answer type questions, two from
each unit. A candidate will have to attempt four questions selecting one question from each
unit. Each question will carry 12 marks.

Reference Books

1. Understanding Enzymes- Trevor, P., Prentice Hall/Ellis, Harwood, England, 4th edition (2018).
2. Fundamentals of Enzymology- Nicholas, C. Price and Lewis Stevens, Oxford University Press,
3rd edition (2009).
3. Principles of Fermentation Technology- Stanbury, P.F., Whitaker, A., & Hall, S.J. India:
Elsevier Science Ltd, 3rd edition (2016).
rd
4. Bioprocess Engineering: Basic concepts- Shuler, M. L., &Kargi, K. India: Prentice Hall, 3
edition (2017).
rd
5. Introduction to Plant Biotechnology- Chawla, H. S., C RC Press, 3 edition (2020).
6. Text Book of Biotechnology- Das, H.K., Wiley India Pvt. Limited, 5th edition (2017).
7. Plant Biotechnology- Hammound, J., McGarvey, P. and Yusibov, V., Springer Verag (2012).
8. Plant Biotechnology- Gupta, P.K.,Rastogi Publishers, Meerut, India, 2nd edition (2016).
9. Plant Biotechnology and Genetics- Steward CN. Techniques and Applications, Wiley and Sons,
2nd edition (2016).
10. Principles of Gene manipulation- Old, R.N. and Primose, S.B.., Blackwell Publishing,
7thedition (2006).
11. Goldsby, R. A., Kindt, T.J. and Osborne,B.A.Kuby Immunology. W.H. Freeman and company,
New York, 7th edition, (2013).
12. Roitt,I., Brostoff, J. and Male,D. Immunology. Hartcourt Brace and Company, Asia
Pvt.Ltd.latest edition.
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – II
(Examination to be held in May 2023, 2024, 2025)
MINOR COURSE

Course Code: UMIBTT-202

Course Title: Introduction of Biotechnology-II
Credits: 4 (3Theory+1Practical)
Total No. of Lectures: Theory: 45 hours
Practical: 30 hours
Maximum Marks: 100
Theory: 75
Practical: 25
Duration of Examination: 3 hours

Objectives and Expected Learning Outcomes:

The course provides an introduction to the fundamentals of Biotechnology with the major focus to
recombinant DNA technology, Immunology, Enzyme and Bioprocess technology; plant and animal
biotechnology. After successfully completing this course, the students will be able to understand the
basic concepts in rDNA technology and Bioprocess technology and also understand the process of
immune system and mechanism of enzyme action.

Unit 1: Recombinant DNA technology

Concept of recombinant DNA technology and its enzymes in gene cloning; Method of gene cloning
and tools and techniques involved, Applications of recombinant DNA technology in medicine,
agriculture, veterinary sciences and protein engineering.

Unit 2: Immunology
Brief history and overview of immune system, Innate and Adaptive immunity, Active and Passive
immunity, cells and Hematopoiesis, organs of immune system, Concept of Antigen and Antibody,
Immunoglobulin and their types, monoclonal antibody generation, MHC complex, Antigen-
antibody interactions and assays used for detection (RIA and ELISA).
Unit 3: Enzyme and Bioprocess Technology
Introduction to Enzymes, Nomenclature and classification of Enzymes, brief introduction to active
site. Cofactors, coenzyme, prosthetic groups, holoenzyme and apoenzyme, Active site hypothesis
(lock-and-key, induced fit, strain or transition-state stabilization); Enzyme activity units, Factors
affecting enzyme activity (Enzyme concentration, substrate concentration, pH, temperature and
reaction time). Introduction to Bioprocess Technology, Types of bioprocesses- batch, continuous,
fed batch, Microbial growth kinetics Design and formulation of Media for industrial bioprocesses,
Sterilization of media and air, Bioreactor and its types.

Unit 4: Plant and Animal Biotechnology

Animal tissue culture, history, requirements for animal cell culture, aseptic conditions, types of
media, Primary and secondary culture, subculture, cryopreservation. In vitro culture: approaches &
methodologies - preparation steps for tissue culture; surface sterilization of plant material; basic
procedure for aseptic tissue transfer; incubation of culture; media composition & Growth hormones;
micro-culture of plants.
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – II
(Examination to be held in May 2023, 2024, 2025)
MINOR COURSE

Course Code: UMIBTT-202

Course Title: Introduction of Biotechnology-II
Credits: 4 (3Theory+1Practical)
Total No. of Lectures: Theory: 45 hours
Practical: 30 hours
Maximum Marks: 100
Theory: 75
Practical: 25
Duration of Examination: 3 hours

Practicals

1. PCR Amplification of gene of interest

2. Cloning of gene of interest in plasmid
3. Understanding the concept and maintenance of aseptic conditions for animal cell culture lab
4. Preparation of the media
5. Demonstration of various sterilization techniques used in laboratory.
6. Preparation of culture media for tissue culture, bacteria and fungi.
7. Demonstration of various steps of micropropagation
8. Effect of reaction time on the activity of enzyme.
9. Effect of substrate concentration on enzyme activity,
10. Calculation of kinetic parameters such as Km, Vmax.
11. Effect of temperature and pH on the activity of enzyme and pH and thermostability analysis
12. To perform ABO blood typing
13. To perform differential leukocyte count

NOTE FOR PAPER SETTING

Examination Theory / Syllabus to be covered Time Allotted for % Weightage (Marks)

Practical in the Examination Exam
Mid Term Assessment 50% 1 ½ Hours 15
test
External Theory End 100% 3 Hours 60
Semester
Internal Practical - - 10
(Based on Daily
Performance only)
External Practical - - 15
A) Mid Term Assessment test: (15 Marks) Time Allotted 1 ½ Hours

B) External End Semester Examination: (60 Marks) Time Allotted 3 Hours

a) External End Semester Theory Examination will have two sections (A & B).
b) Section A shall be of 12 Marks and will comprise of 4 short answer type questions one
question from each unit carrying 03 Marks each. A candidate will have to attempt all the
questions.
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – II
(Examination to be held in May 2023, 2024, 2025)
MINOR COURSE

Course Code: UMIBTT-202

Course Title: Introduction of Biotechnology-II
Credits: 4 (3Theory+1Practical)
Total No. of Lectures: Theory: 45 hours
Practical: 30 hours
Maximum Marks: 100
Theory: 75
Practical: 25
Duration of Examination: 3 hours

c) Section B shall be of 48 Marks and will comprise of 8 long answer type questions, two from
each unit. A candidate will have to attempt four questions selecting one question from each
unit. Each question will carry 12 marks.

Reference Books

1. Understanding Enzymes- Trevor, P., Prentice Hall/Ellis, Harwood, England, 4th edition (2018).
2. Fundamentals of Enzymology- Nicholas, C. Price and Lewis Stevens, Oxford University Press,
3rd edition (2009).
3. Principles of Fermentation Technology- Stanbury, P.F., Whitaker, A., & Hall, S.J. India:
Elsevier Science Ltd, 3rd edition (2016).
4. Bioprocess Engineering: Basic concepts- Shuler, M. L., &Kargi, K. India: Prentice Hall, 3rd
edition (2017).
5. Introduction to Plant Biotechnology- Chawla, H. S., C RC Press, 3rd edition (2020).
6. Text Book of Biotechnology- Das, H.K., Wiley India Pvt. Limited, 5th edition (2017).
7. Plant Biotechnology- Hammound, J., McGarvey, P. and Yusibov, V., Springer Verag (2012).
8. Plant Biotechnology- Gupta, P.K., Rastogi Publishers, Meerut, India, 2nd edition (2016).
9. Plant Biotechnology and Genetics- Steward CN. Techniques and Applications, Wiley and Sons,
2nd edition (2016).
10. Principles of Gene manipulation- Old, R.N. and Primose, S.B.., Blackwell Publishing,
7thedition (2006).
11. Goldsby, R. A., Kindt, T.J. and Osborne,B.A.Kuby Immunology. W.H. Freeman and company,
New York, 7th edition, (2013).
12. Roitt,I., Brostoff, J. and Male,D. Immunology. Hartcourt Brace and Company, Asia Pvt.Ltd.
latest edition.
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – II
(Examination to be held in May 2023, 2024, 2025)
MULTIDISCIPLINARY COURSE

Course Code: UMDBTT-203

Course Title: Applications of Biotechnology in Agriculture
Credits: 3
Total No. of Lectures: Theory: 45 hours
Maximum Marks: 75
Theory: 75
Duration of Examination: 3 hours

Objectives and Expected Learning Outcomes

Biotechnology has huge is increasingly being used to in different areas of Agriculture for enhancing
quality and quantity, disease and stress resistance in crops and livestock. At the end of the course
students will able to understand the principals and technical advances behind the in vitro culture of
plant cells and methods of producing transgenics; improving the productivity and performance of
plants under biotic and abiotic stresses; development of transgenic animals for the production of
vaccines, milk, and for poultry and livestock improvement.

Unit 1: Introduction to Agricultural Biotechnology

Conventional breeding and Biotechnology, Use of Biotechnology in different areas of agriculture:
Biofertilizers, Biopesticides. Introduction to GM crops, their safety and acceptance; role of
Biotechnology in climate change and biodiversity

Unit II: Introduction to Plant Tissue Culture

History of plant tissue culture, requirements for plant tissue culture lab, Totipotency, micro
propagation of economically important crops, Artificial Seeds.

UnitIII: Transgenic Plants and Applications

Basic concept and techniques of genetic transformation,Genetic Engineering for quality
improvement, Disease resistance plants: herbicide resistance, disease resistance; transgenic crops
and food security; Plants as bio factories for molecular pharming; plantibodies, nutraceuticals,
ethical issues associated with transgenic crops.

Unit IV: Transgenic Animals and Applications

Introduction to Animal Biotechnology, Role of Animal Biotechnology in development of disease
resistance, better milk and meat yielding animals, fisheries and poultry improvement, vaccines,
ethical issues associated with transgenic animals.

NOTE FOR PAPER SETTING

Examination Syllabus to be Time Allotted for % Weightage (Marks)

Theory/Practical covered in the Exam
Examination
Internal Theory 50% 1½ Hours 15
Assessment
External Theory End 100% 3 Hours 60
Semester
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – II
(Examination to be held in May 2023, 2024, 2025)
MULTIDISCIPLINARY COURSE

Course Code: UMDBTT-203

Course Title: Applications of Biotechnology in Agriculture
Credits: 3
Total No. of Lectures: Theory: 45 hours
Maximum Marks: 75
Theory: 75
Duration of Examination: 3 hours

A) Mid Term Assessment test: (15 Marks) Time Allotted 1 ½ Hours

B) External End Semester Examination: (60 Marks) Time Allotted 3 Hours

a) External End Semester Theory Examination will have two sections (A & B).
b) Section A shall be of 12 Marks and will comprise of 4 short answer type questions one
question from each unit carrying 03 Marks each. A candidate will have to attempt all the
questions.
c) Section B shall be of 48 Marks and will comprise of 8 long answer type questions, two from
each unit. A candidate will have to attempt four questions selecting one question from each
unit. Each question will carry 12 marks.

Reference Books

1. Plant Tissue Culture: Theory and Practice- Bhojwani, S.S and Razdan, M.K., Elsevier Science
(2010).
2. Plant Cell and Tissue Culture for the Production of Food Ingredients- Fu, T-J., Singh, G. and
Curitis, W.R., Kluwer Academic/ Plenum Press, latest edition.
3. Elements of Biotechnology- Gupta, P.K., Rastogi Publications, Merrut, India, 2nd edition (2019).
4. Introduction to Plant Biotechnology- Chawla, H. S. C RC Press, 3rd edition (2020).
5. Text Book of Biotechnology- Das, H.K.,Wiley India Pvt. Limited, 5th edition (2017).
6. Plant Biotechnology- Hammound, J., McGarvey, P. and Yusibov, V., Springer Verag (2012).
7. Plant Biotechnology- Gupta, P.K. Rastogi Publishers, Meerut, India, 2ndedition (2016).
8. Plant Biotechnology and Genetics- Steward CN., Wiley and Sons, 2nd edition (2016).
9. Principles of Gene manipulation- Old, R.N. and Primose, S.B., Blackwell Publishing,7thedition
(2006).
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – II
(Examination to be held in May 2023, 2024, 2025)
SKILL ENHANCEMENT COURSE

Course Code: USEBTT-204

Course Title: Methods in Microbiology and Biochemistry
Credits: 2 (40 marks theory+ 10 marks practical)
Total No. of Lectures: Theory: 25 hours
Practical: 30 hours
Maximum Marks: 50
Theory: 40
Practical: 10
Duration of Examination: 2.5 hours

Objectives and Expected Learning Outcomes: The course provides the basic concept of various
methods used in microbiology and biochemistry. After successfully completing this course, the
students will be able to performs various microbiological processes like culturing, identification,
purification and preservation of microbes. The student will be able to perform various biochemical
tests, Blood grouping, sputum analysis etc

Unit 1: Methods in Microbiology

Microscopy: Principle and working of light microscope, compound microscope, Dark field
microscope, Phase contrast microscope, Fluorescence Microscope, confocal microscope and
electron Microscopes, Culture media and pure culture techniques: Components of media, natural
and synthetic media, chemically defined media, complex media, selective, differential, indicator,
enriched and enrichment media, Serial dilution and plating methods (pour plate, spread plate, streak
plate methods).
Control of microbes- Sterilisation, disinfection, antiseptic, tyndallisation, pasteurization: Physical-
dry heat, moist heat, UV light, ionizing radiation, filtration, HEPA filter, Chemical-phenol and
phenolic compounds, (halogen aliphatic alcohol, formaldehyde, ethylene oxide, heavy metals)
anionic and cationic detergents. Culture preservation and maintenance: sub-culturing, refrigeration,
glycerol preservation, stab cultures, cryopreservation, lyophilization and paraffin method, Stains
and staining techniques: Principle of staining, Types of stains-simple staining, negative staining,
differential staining, Gram and acid-fast staining, flagellar staining, capsule and endospore staining.

Unit 2: Methods in Biochemistry

Water, Electrolyte and Acid-Base Balance. Distribution of Water in Body, Water turnover and
balance. Body Fluids: Biochemistry of Urine, blood and CSF Normal and abnormal constituents
and clinical entities in body fluids. Clinical Hematology: Collection of blood - Anticoagulant,
preservation, Estimation of blood glucose, Clotting time, bleeding time Blood grouping- ABO
system, ABO Grouping, Rh typing, CSF and other body Fluids Cerebrospinal fluid analysis, Semen
analysis, sputum examination, pregnancy test – Interpretation.

Unit 3: Practical

1. To study the use and maintenance of compound microscope

2. Preparation of different types of culture media and sterilization
3. Sub-culturing and preservation of cultures
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – II
(Examination to be held in May 2023, 2024, 2025)
SKILL ENHANCEMENT COURSE

Course Code: USEBTT-204

Course Title: Methods in Microbiology and Biochemistry
Credits: 2 (40 marks theory+ 10 marks practical)
Total No. of Lectures: Theory: 25 hours
Practical: 30 hours
Maximum Marks: 50
Theory: 40
Practical: 10
Duration of Examination: 2.5 hours

4. Aseptic techniques- Culture transfer from solid to solid, solid to liquid and liquid to liquid:
Checking of possible contamination
5. Isolation of microorganisms from soil, air and water.
6. Colony purification, bacterial staining: simple staining, Negative staining and Gram’s
staining
7. Viable count of bacteria by serial dilution and pour plating.
8. Simple biochemical tests of bacteria
9. Antibiotic sensitivity tests
10. Operation of autoclave, hot air oven, membrane filtration (demonstration only), culture
room fumigation using formalin, surface sterilization
11. Preparation of normal and molar solutions, Stock and working solutions
12. Preparation of physiological buffers
13. Qualitative test for detection and quantitative estimation of glucose in the solution.
14. Qualitative test for detection and quantitative estimation of proteins in the solution

NOTE FOR PAPER SETTING

Total marks of the USEBTT-201 are 50. 20% marks shall be reserved for internal assessment (10
Marks). 80% of the marks (40 marks) shall be reserved for external examination to be conducted by
the University/College.

Internal Assessment Test (10 Marks) Time Allotted 1 Hour.

External End Semester Examination 40 Marks (Time Allotted 2½ Hours)

1. External theory exam shall be of 30 marks and consists of 2 sections.
a. Section A shall be of 10 marks and comprise of 4 short answer type questions of 2½
marks each, from Unit I and II (All compulsory).
b. Section B shall be of 20 marks and will comprise of four long type questions of 10
marks each, two from Unit I and two from Unit II. A candidate has to attempt one from
each unit.
2. External Practical Exam shall be of 10 marks to be conducted by the college with practical
exercise of 5 marks and Viva voce of 5 marks.
Note: During the submission of marks to University of Jammu final pattern will be 10 (internal) and
40 (external).
 University of Jammu
Syllabi of Biotechnology for FYUP under CBCS as per NEP-2020
Semester – II
(Examination to be held in May 2023, 2024, 2025)
SKILL ENHANCEMENT COURSE

Course Code: USEBTT-204

Course Title: Methods in Microbiology and Biochemistry
Credits: 2 (40 marks theory+ 10 marks practical)
Total No. of Lectures: Theory: 25 hours
Practical: 30 hours
Maximum Marks: 50
Theory: 40
Practical: 10
Duration of Examination: 2.5 hours
Reference books

1. Methods in biotechnology- Hong, S. B., Rashid, M. B., & Santiago-Vázquez, L. Z. John,

Wiley & Sons, 1st edition (2016).
2. Wilson and Walker's principles and techniques of biochemistry and molecular biology-
Wilson, K., Hofmann, A., Walker, J. M., &Clokie, S., Cambridge University Press, 8th edition
(2018).
3. Analytical techniques in biochemistry and molecular biology- Katoch, R., Springer Science &
Business Media (2011).
4. Biochemical methods- Sadasivam, and Manickam, New age International Publishers, 3rd
edition (2018).
5. Basic Methods in Microscopy: Protocols and Concepts from Cells: a Laboratory Manual-
Goldman, R. D., & Spector, D. L., Cold Spring Harbor Laboratory Press, 1st edition (2006).
6. Fundamentals of bioanalytical techniques and instrumentation- Ghosal, S., &Avasthi, A. S.
Phi Learning Pvt. Ltd, 2nd edition (2018).