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Experiment 1

The document describes procedures for preparing stained temporary mounts of onion peel and human cheek cells under a microscope. It provides materials, steps to stain and mount each sample, observations to record, inferences to draw, and precautions to follow. Diagrams of the cells from each sample are to be drawn based on microscope observations.

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Nouf Naqvi
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29 views

Experiment 1

The document describes procedures for preparing stained temporary mounts of onion peel and human cheek cells under a microscope. It provides materials, steps to stain and mount each sample, observations to record, inferences to draw, and precautions to follow. Diagrams of the cells from each sample are to be drawn based on microscope observations.

Uploaded by

Nouf Naqvi
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Experiment 1-

Objective- To prepare stained temporary mounts of

A. onion peel and

B. human cheek cells and to record observations and draw their labelled diagrams.

(A) To prepare stained temporary mount of onion peel.

Requirements

A medium sized onion, clean glass slide, cover slip, watch glass or petridish, a pair of scissors, a
knife, needle, brush, distilled water, glycerine, dropper, blotting paper, safranin solution, a high-
powered microscope.

Procedure

1. Take a medium sized onion, cut its outer surface with knife.
2. Use forceps to remove the peel of onion.
3. With the help of needle separate the small portion of epidermis (peel).
4. Keep dilute safranin solution in a watch glass.
5. Put this small peel in this watch glass with brush and allow it to stain for 3-5 minutes.
6. Transfer the stained peel to another watch glass that contains distilled water in it, to remove
extra stain.
7. Take a clean dry slide and place two drops of water/glycerine on the centre of the slide.
8. Transfer the stained peel with needle and brush on the middle of the slide, if the peel curls
straighten it and flatten it with brush and needle, do this gently.
9. With the help of blade cut the peel into a square shape.
10. Take a dry and clean coverslip and gently place it on the slide with the help of needle such
that no air bubbles enter in it.
11. Gently press the coverslip with needle for even spreading of glycerine.
12. Remove the extra stain and water with the help of blotting paper.
13. Clean the sides of the coverslip with dry blotting paper and place it under the lens of the
microscope and record your observations.

Observations
Write about your observations in the lab.

Inference
Plant cell shows the following:

Draw inference based on the observations made


Precautions

1. Use dilute stain for staining.


2. Avoid the formation of air-bubbles while placing the coverslip on the slide.
3. Take very thin peel of onion to get a single layer of cells, no overlapping of cells should be
seen.
4. Use dry and clean slide, wipe out extra stain or water present on the sides of the slide.

Draw a figure of an onion peel based on the observation made under a microscope and
include the figure in the interleaf page of the record book.

B. To prepare stained, temporary mount of human cheek cells.

Materials Required
Slide, coverslip, watch glass, methylene blue stain, blotting paper, toothpick, needle, dropper,
brush, microscope and glycerine.

Procedure-

1. Make a dilute methylene blue solution in a watch glass.


2. Keep a clean slide with a drop of distilled water at the middle of the slide.
3. Take a clean/unused toothpick and scrap the inner wall of your mouth/cheek gently to obtain
the epithelial animal tissue, (use the blunt side of toothpick)
4. Transfer the scrap on the middle of the glass slide and put a drop of methylene blue solution
on it, to stain the cells.
5. After 2-3 minutes place the coverslip gently on the cheek cell with the help of needle and
avoid the air bubble. (A drop of glycerine can be spread on the cheek cells, it is optional)
6. With the help of blotting paper remove the extra stain/water present on the slide.
7. Place the slide under microscope and observe it.

Observations

Write about your observations in the lab.

Inference
Draw inference based on the observations made
Precautions

1. Use unused/new toothpick for scraping of cheek cells.


2. Placing of coverslip should be done carefully to avoid air bubbles.
3. Avoid overstaining.
4. Use clean/dry mounted slide while placing it under the lens of the microscope.
5. Avoid overlapping of the cells.

Draw a figure of an animal cell based on the observation made under a microscope and
include the figure in the interleaf page of the record book.

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