Agilent 1260 Infinity

Preparative Autosampler

User Manual
Notices
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 Contents

Contents

1 Introduction to the Autosampler 7

Introduction to the Autosampler 8
Sampling Sequence 10
Sampling Unit 13
Transport Assembly 16
Early Maintenance Feedback (EMF) 18
Electrical Connections 19
Agilent 1200 Infinity Interfaces 21

2 Site Requirements and Specifications 23

Site Requirements 24
Physical Specifications 27
Performance Specifications 28

3 Installing the Autosampler 33

Unpacking the Autosampler 34
Optimizing the Stack Configuration 37
Installing the Autosampler 41
Installing the Thermostatted Autosampler 44
Flow Connections 48
Installing the Sample Tray 50
Transporting the Autosampler 51

4 Using the Autosampler 53

Solvent Information 54
Sample Trays 56
Choice of Vials and Caps 58

5 Optimizing Performance 61
Optimization for Lowest Carry-over 62
Fast Injection Cycle and Low Delay Volume 66
Precise Injection Volume 68

3
Contents

Choice of Rotor Seal 70

6 Troubleshooting and Diagnostics 71

Agilent Lab Monitor & Diagnostic Software 72
Overview of the Sampler’s Indicators and Test Functions 73

7 Maintenance 75
Introduction into Maintenance and Repair 76
Overview of Main Repair Procedures 80
Early Maintenance Feedback (EMF) 82
Maintenance Functions 84
Simple Repairs 85

8 Parts and Materials for Maintenance 105

Main Assemblies 106
Analytical-Head Assembly 108
Vial Trays 111
Standard Autosampler Accessory Kit G1329-68725 112
Preparative Autosampler Accessory Kit G2260-68705 113
Maintenance Kit G1313-68730 for G1329A 114
Maintenance Kit G1313-68719 for G1329B 115
Multi-Draw Kit G1313-6871 116
900 µl Injection Upgrade Kit G1363A for G1329A 117
External Tray G1313-60004 118

9 Identifying Cables 119

Cable Overview 120
Analog Cables 122
Remote Cables 125
BCD Cables 130
External Contact Cable 132
CAN/LAN Cables 133
Auxiliary Cable 134
RS-232 Cables 135

4
 Contents

10 Appendix 137
General Safety Information 138
Lithium Batteries Information 142
Radio Interference 143
Sound Emission 144
Agilent Technologies on Internet 145

5
Contents

6
1
Introduction to the Autosampler
Introduction to the Autosampler 8
Sampling Sequence 10
Injection Sequence 11
Sampling Unit 13
Needle-Drive 14
Analytical head / preparative head 14
Injection-Valve 15
Transport Assembly 16
Early Maintenance Feedback (EMF) 18
Electrical Connections 19
Agilent 1200 Infinity Interfaces 21

Agilent Technologies 7
1 Introduction to the Autosampler
Introduction to the Autosampler

Introduction to the Autosampler

Three models of Agilent 1200 Infinity Series autosamplers are available;

within this introduction they will be referred to as the standard autosampler
(G1329A), the standard autosampler SL (G1329B) and the preparative
autosampler (G2260A). Unless otherwise stated all information in this section
is valid for all models.
The Agilent 1100 Series autosamplers and Agilent 1200 Infinity Series
autosamplers are designed for use with other modules of the Agilent 1200
Infinity Series LC system, with the HP 1050 Series, or with other LC systems if
adequate remote control inputs and outputs are available. The autosamplerss
are controlled from the Agilent 1200 Infinity control module (G4208 A Instant
Pilot) or from the Agilent ChemStation for LC.
Three sample-rack sizes are available for the autosamplers. The standard
full-size rack holds 100 × 1.8 ml vials, while the two half-size racks provide
space for 40 × 1.8 ml vials and 15 × 6 ml vials respectively. Any two half-size
rack trays can be installed in the autosamplers simultaneously. A specially
designed sample-rack holding 100 × 1.8 ml vials is available for use with
thermostatted autosamplers. The half-size racks trays are not designed for an
optimal heat transfer when they are used with a thermostatted autosampler.
The autosamplers transport mechanism uses an X-Z-Theta movement to
optimize vial pick-up and return. Vials are picked up by the gripper arm, and
positioned below the sampling unit. The gripper transport mechanism and
sampling unit are driven by motors. Movement is monitored by optical sensors
and optical encoders to ensure correct operation. The metering device is
always flushed after injection to ensure minimum carry-over.
The standard analytical head device provides injection volumes from
0.1 – 100 µl. Two preparative head devices provide injection volumes from
0.1 – 900 µl. One head is limited by a system pressure of 200 bars, the other by
a system pressure of 400 bars. The G1329B autosampler SL uses an analytical
head providing injection volumes from 0.1 – 100 µl for pressures up to 600 bar
as used in rapid resolution systems.
The six-port injection valve unit (only 5 ports are used) is driven by a
high-speed hybrid stepper motor. During the sampling sequence, the valve unit
bypasses the autosamplers, and directly connects the flow from the pump to

8
 Introduction to the Autosampler 1
Introduction to the Autosampler

the column. During injection and analysis, the valve unit directs the flow
through the autosamplers which ensures that the sample is injected
completely into the column, and that any sample residue is removed from the
metering unit and needle from before the next sampling sequence begins.
Different valves are available for the standard and preparative autosamplers.
Control of the vial temperature in the thermostatted autosampler is achieved
using an additional Agilent 1200 Infinity module; the ALS thermostat. Details
of this module are given in the Agilent 1200 Infinity thermostatted
autosampler Supplemental Manual.

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Figure 1 Overview of the Autosampler

9
1 Introduction to the Autosampler
Sampling Sequence

Sampling Sequence

The movements of the autosampler components during the sampling sequence

are monitored continuously by the autosampler processor. The processor
defines specific time windows and mechanical ranges for each movement. If a
specific step of the sampling sequence can’t be completed successfully, an
error message is generated.
Solvent is bypassed from the autosamplers by the injection valve during the
sampling sequence. The sample vial is selected by a gripper arm from a static
sample rack, or from external vial positions. The gripper arm places the
sample vial below the injection needle. The required volume of sample is
drawn into the sample loop by the metering device. Sample is applied to the
column when the injection valve returns to the mainpass position at the end of
the sampling sequence.
The sampling sequence occurs in the following order:
1 The injection valve switches to the bypass position.
2 The plunger of the metering device moves to the initialization position.
3 The gripper arm moves from the home position, and selects the vial. At the
same time, the needle lifts out of the seat.
4 The gripper arm places the vial below the needle.
5 The needle lowers into the vial.
6 The metering device draws the defined sample volume.
7 The needle lifts out of the vial.
8 If the automated needle wash is selected (see “Using the Automated Needle
Wash” on page 63), the gripper arm replaces the sample vial, positions the
wash vial below the needle, lowers the needle into the vial, then lifts the
needle out of the wash vial.
9 The gripper arm checks if the safety flap is in position.
10 The gripper arm replaces the vial, and returns to the home position.
Simultaneously, the needle lowers into the seat.
11 The injection valve switches to the mainpass position.

10
 Introduction to the Autosampler 1
Sampling Sequence

Injection Sequence
Before the start of the injection sequence, and during an analysis, the injection
valve is in the mainpass position (Figure 2 on page 11). In this position, the
mobile phase flows through the autosamplers metering device, sample loop,
and needle, ensuring all parts in contact with sample are flushed during the
run, thus minimizing carry-over.

Figure 2 Mainpass Position

When the sample sequence begins, the valve unit switches to the bypass
position (Figure 3 on page 11). Solvent from the pump enters the valve unit at
port 1, and flows directly to the column through port 6.

Figure 3 Bypass Position

11
1 Introduction to the Autosampler
Sampling Sequence

Next, the needle is raised, and the vial is positioned below the needle. The
needle moves down into the vial, and the metering unit draws the sample into
the sample loop (Figure 4 on page 12).

Figure 4 Drawing the Sample

When the metering unit has drawn the required volume of sample into the
sample loop, the needle is raised, and the vial is replaced in the sample tray.
The needle is lowered into the needle seat, and the injection valve switches
back to the mainpass position, flushing the sample onto the column (Figure 5
on page 12).

Figure 5 Mainpass Position (Sample Injection)

12
 Introduction to the Autosampler 1
Sampling Unit

Sampling Unit

The sampling unit comprises three main assemblies: needle drive, metering
device, and injection valve.

The replacement sampling unit excludes the injection valve and metering head assemblies.
NOTE

The sampling units for the standard and the preparative autosamplers are
different.

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Figure 6 Autosampler Sampling Unit

13
1 Introduction to the Autosampler
Sampling Unit

Needle-Drive
The needle movement is driven by a stepper motor connected to the spindle
assembly by a toothed belt. The circular motion of the motor is converted to
linear motion by the drive nut on the spindle assembly. The upper and lower
needle positions are detected by reflection sensors on the sampling unit flex
board, while the needle-in-vial position is determined by counting the motor
steps from the upper needle-sensor position.

Analytical head / preparative head

The analytical head is driven by the stepper motor connected to the drive shaft
by a toothed belt. The drive nut on the spindle converts the circular movement
of the spindle to linear motion. The drive nut pushes the sapphire plunger
against the tension of the spring into the analytical head. The base of the
plunger sits on the large bearing of the drive nut, which ensures the plunger is
always centered. A ceramic ring guides the movement of the plunger in the
analytical head. The home position of the plunger is sensed by an infra-red
sensor on the sampling unit flex board, while the sample volume is determined
by counting the number of steps from the home position. The backward
movement of the plunger (driven by the spring) draws sample from the vial.

Table 1 Analytical Head Technical Data

Standard (100 µl Standard (900 µl) Preparative (900 µl)

Number of steps 15000 15000 15000

Volume resolution 7 nl/motor step 60 nl/motor step 60 nl/motor step

Maximum stroke 100 µl 900 µl 900 µl

Pressure limit 600 bar 200 bar 400 bar

Plunger material Sapphire Sapphire Sapphire

14
 Introduction to the Autosampler 1
Sampling Unit

Injection-Valve
The two-position 6-port injection valve is driven by a stepper motor. Only five
of the six ports are used (port 3 is not used). A lever/slider mechanism
transfers the movement of the stepper motor to the injection valve. Two
microswitches monitor switching of the valve (bypass and mainpass end
positions).
No valve adjustments are required after replacing internal components.

Table 2 Injection-Valve Technical Data

Standard Preparative MBB™ Autosampler SL

Motor type 4 V, 1.2 A stepper motor 4 V, 1.2 A stepper motor 4 V, 1.2 A stepper motor

Seal material Vespel™ (Tefzel™ PEEK PEEK

available)

Stator material Ceramic/PEEK PEEK None

Number of ports 6 6 6

Switching time < 150 ms < 150 ms < 150 ms

15
1 Introduction to the Autosampler
Transport Assembly

Transport Assembly

The transport unit comprises an X-axis slide (left-right motion), a Z-axis arm
(up-down motion), and a gripper assembly (rotation and vial-gripping).

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Figure 7 Transport Assembly

The transport assembly uses four stepper motors driven in closed-loop mode
for accurate positioning of the gripper assembly for sample-vial transport. The
rotational movement of the motors is converted to linear motion (X- and
Z-axes) by toothed belts connected to the drive spindles. The rotation (theta
axes) of the gripper assembly is transferred from the motor by a toothed belt
and series of gears. The opening and closing of the gripper fingers are driven
by a stepper motor linked by a toothed belt to the planetary gearing inside the
gripper assembly.

16
 Introduction to the Autosampler 1
Transport Assembly

The stepper motor positions are determined by the optical encoders mounted
onto the stepper-motor housing. The encoders monitor the position of the
motors continually, and correct for position errors automatically (e.g. if the
gripper is accidentally moved out of position when loading vials into the vial
tray). The initialization positions of the moving components are sensed by
reflection sensors mounted on the flex board. These positions are used by the
processor to calculate the actual motor position. An additional six reflection
sensors for tray recognition are mounted on the flex board at the front of the
assembly.

17
1 Introduction to the Autosampler
Early Maintenance Feedback (EMF)

Early Maintenance Feedback (EMF)

The early maintenance feedback (EMF) feature monitors the usage of specific
components in the instrument, and provides feedback when the user-setable
limits have been exceeded. The visual feedback in the user interface provides
an indication that maintenance procedures should be scheduled.
For details on EMF counters and how to use them, see “Early Maintenance
Feedback (EMF)” on page 82.

18
 Introduction to the Autosampler 1
Electrical Connections

Electrical Connections

• The GPIB connector is used to connect the module with a computer. The
address and control switch module next to the GPIB connector determines
the GPIB address of your module. The switches are preset to a default
address and is recognized once after power is switched ON.
• The CAN bus is a serial bus with high speed data transfer. The two
connectors for the CAN bus are used for internal Agilent 1200 Infinity
module data transfer and synchronization.
• One analog output provides signals for integrators or data handling
systems.
• The interface board slot is used for external contacts and BCD bottle
number output or LAN connections.
• The REMOTE connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features such as
start, stop, common shut down, prepare, and so on.
• With the appropriate software, the RS-232C connector may be used to
control the module from a computer through a RS-232C connection. This
connector is activated and can be configured with the configuration switch.
See your software documentation for further information.
• The power input socket accepts a line voltage of 100 – 240 volts AC ± 10%
with a line frequency of 50 or 60 Hz. Maximum power consumption is
300 VA. There is no voltage selector on your module because the power
supply has wide-ranging capability. There are no externally accessible
fuses, because automatic electronic fuses are implemented in the power
supply. The security lever at the power input socket prevents the module
cover from being taken off when line power is still connected.

Never use cables other than the ones supplied by Agilent Technologies to ensure proper
NOTE functionality and compliance with safety or EMC regulations.

19
1 Introduction to the Autosampler
Electrical Connections

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Figure 8 Autosampler (plus Thermostat) Electrical Connections

20
 Introduction to the Autosampler 1
Agilent 1200 Infinity Interfaces

Agilent 1200 Infinity Interfaces

The Agilent 1200 Infinity modules provide the following interfaces:

Table 3 Agilent 1200 Infinity Interfaces

Interface Type Pumps Autosampler DA Detector VW Detector Thermostatted Vacuum

MW Detector RI Detector Column Degasser
FL Detector Compartment

CAN Yes Yes Yes Yes Yes No

GPIB Yes Yes Yes Yes Yes No

RS-232C Yes Yes Yes Yes Yes No

APG Remote Yes Yes Yes Yes Yes Yes

Analog Yes No 2× 1× No Yes1

Interface board2 Yes Yes Yes Yes No No

1
The vacuum degasser will have a special connector for specific use. For details, see the degasser manual.
2
The interface board slot (not common to all modules) provides specific interfacing needs (external con-
tacts, BCD, LAN and so on).

21
1 Introduction to the Autosampler
Agilent 1200 Infinity Interfaces

22
2
Site Requirements and Specifications
Site Requirements 24
Power Consideration 24
Power Cords 25
Bench Space 26
Environment 26
Physical Specifications 27
Performance Specifications 28

Agilent Technologies 23
2 Site Requirements and Specifications
Site Requirements

Site Requirements

A suitable environment is important to ensure optimum performance of the

instrument.

Power Consideration
The autosampler power supply has wide-ranging capability (see Table 4 on
page 27). Consequently there is no voltage selector in the rear of the
autosampler. There are also no externally accessible fuses, because automatic
electronic fuses are implemented in the power supply.
The thermostatted autosampler comprises two modules, the standard or
preparative autosampler and the thermostat (G1330B). Both modules have a
separate power supply and a power plug for the line connections. The two
modules are connected by a control cable and both are turned on by the
autosampler module.

Damaged electronics
WA R N I N G
Disconnecting or reconnecting the sampler to thermostat cable when the power
cords are connected to either of the two modules will damage the electronics of the
modules.
➔ Make sure the power cords are unplugged before disconnecting or reconnecting the
sampler to thermostat cable.

Incorrect line voltage at the instrument

WA R N I N G
Shock hazard or damage of your instrumentation can result, if the devices are
connected to a line voltage higher than specified.
➔ Connect your instrument to the specified line voltage.

24
 Site Requirements and Specifications 2
Site Requirements

Unaccessable power plug.

CAUTION
In case of emergency it must be possible to disconnect the instrument from the power
line at any time.
➔ Make sure the power connector of the instrument can be easily reached and
unplugged.
➔ Provide sufficient space behind the power socket of the instrument to unplug the
cable.

Power Cords
Different power cords are offered as options with the module. The female end
of all power cords is identical. It plugs into the power-input socket at the rear
of the module. The male end of each power cord is different and designed to
match the wall socket of a particular country or region.

The absence of ground connection and the use of an unspecified power cord can
WA R N I N G
lead to electric shock or short circuit.
Electric Shock
➔ Never operate your instrumentation from a power outlet that has no ground
connection.
➔ Never use a power cord other than the Agilent Technologies power cord designed
for your region.

Use of unsupplied cables

WA R N I N G
Using cables not supplied by Agilent Technologies can lead to damage of the
electronic components or personal injury.
➔ Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.

25
2 Site Requirements and Specifications
Site Requirements

Bench Space
The autosampler dimensions and weight (see Table 4 on page 27) allow the
instrument to be placed on almost any laboratory bench. The instrument
requires an additional 2.5 cm (1.0 inch) of space on either side, and
approximately 8 cm (3.1 inches) at the rear for the circulation of air, and room
for electrical connections. Ensure the autosampler is installed in a horizontal
position.
The thermostatted autosampler dimensions and weight allow the instrument
to be placed on almost any laboratory bench. The instrument requires an
additional 25 cm (10 inches) of space on either side for the circulation of air,
and approximately 8 cm (3.1 inches) at the rear for electrical connections.
Ensure the autosampler is installed in a level position.
If a complete Agilent 1200 Infinity Series system is to be installed on the
bench, make sure that the bench is designed to carry the weight of all the
modules. For a complete system including the thermostatted autosampler it is
recommended to position the modules in two stacks, see “Optimizing the Stack
Configuration” on page 37. Make sure that in this configuration there is 25 cm
(10 inches) space on either side of the thermostatted autosampler for the
circulation of air.

Environment
Your module will work within specifications at ambient temperatures and
relative humidity as described in Table 4 on page 27.

Condensation within the module

CAUTION
Condensation will damage the system electronics.
➔ Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
➔ If your module was shipped in cold weather, leave it in its box and allow it to warm
slowly to room temperature to avoid condensation.

26
 Site Requirements and Specifications 2
Physical Specifications

Physical Specifications

Table 4 Physical Specifications

Type Specification Comments

Weight 14.2 kg (32 lbs)

Dimensions 200 × 345 × 435 mm (8 × 13.5 × 17

(width × depth × height) inches)

Line voltage 100 – 240 VAC, ± 10% Wide-ranging capability

Line frequency 50 or 60 Hz, ± 5%

Power consumption 300 VA / 200 W / 683 BTU Maximum

Ambient operating 0–55 °C (32–131 °F) See warning “” on page 27

temperature

Ambient non-operating -40–70 °C (-4–158 °F)

temperature

Humidity < 95%, at 25–40 °C (77–104 °F) Non-condensing

Operating Altitude Up to 2000 m (6500 ft)

Non-operating altitude Up to 4600 m (14950 ft) For storing the module

Safety standards: IEC, CSA, Installation Category II, Pollution For indoor use only. Research
UL Degree 2 Use Only. Not for use in
Diagnostic Procedures.

Hot rear panel

WA R N I N G
Using the autosampler at high environmental temperatures may cause the rear
panel to become hot.
➔ Do not use the autosampler at environmental temperatures higher than 50 °C
(122 °F)

27
2 Site Requirements and Specifications
Performance Specifications

Performance Specifications

Table 5 Performance Specifications Agilent 1200 Infinity Autosampler (G1329A). Valid

when standard 100 µl metering head installed.

Type Specification

Pressure Operating range 0 – 40 MPa (0 – 400 bar, 0 – 5900 psi)

GLP features Early maintenance feedback (EMF), electronic records of

maintenance and errors

Communications Controller-area network (CAN). GPIB (IEEE-448), RS232C,

APG-remote standard, optional four external contact closures and
BCD vial number output

Safety features Leak detection and safe leak handling, low voltages in maintenance
areas, error detection and display

Injection range 0.1 – 100 µl in 0.1 µl increments Up to 1500 µl with multiple draw
(hardware modification required)

Replicate injections 1 – 99 from one vial

Precision < 0.25% RSD from 5 – 100 µl, < 1% RSD 1 – 5 µl

variable volume

Minimum sample volume 1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in

300 µl microvial

Carryover Typically < 0.1%, < 0.05% with external needle cleaning
Sample viscosity range 0.2 – 50 cp

Replicate injections per vial 1 – 99

Sample capacity 100 × 2-ml vials in 1 tray

40 × 2-ml vials in ½ tray
15 × 6-ml vials in ½ tray (Agilent vials only)

Injection cycle time Typically 50 s depending on draw speed and injection volume

28
 Site Requirements and Specifications 2
Performance Specifications

Table 6 Performance Specifications Agilent 1200 Infinity standard autosampler

(G1329A). Valid when standard 900 µl metering head installed.

Type Specification

Pressure Operating range 0 – 20 MPa (0 – 200 bar, 0 – 2950 psi)

GLP features Early maintenance feedback (EMF), electronic records of

maintenance and errors

Communications Controller-area network (CAN). GPIB (IEEE-448), RS232C,

APG-remote standard, optional four external contact closures and
BCD vial number output

Safety features Leak detection and safe leak handling, low voltages in maintenance
areas, error detection and display

Injection range 0.1 – 900 µl in 0.1 µl increments (recommended 1 µl increments) Up

to 1800 µl with multiple draw (hardware modification required)

Replicate injections 1 – 99 from one vial

Precision Typically < 0.5% RSD of peak areas from 5 – 2000 µl, Typically
< 1% RSD of peak areas from 2000 – 5000 µl, Typically < 3% RSD of
peak areas from 1 – 5 µl

Minimum sample volume 1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in

300 µl microvial

Carryover Typically < 0.1%, < 0.05% with external needle cleaning

Sample viscosity range 0.2 – 50 cp

Sample capacity 100 × 2-ml vials in 1 tray

40 × 2-ml vials in ½ tray
15 × 6-ml vials in ½ tray (Agilent vials only)

Injection cycle time 50 s for draw speed 200 µl/min, ejection speed 200 µl/min, injection
volume 5 µl

29
2 Site Requirements and Specifications
Performance Specifications

Table 7 Performance Specifications Agilent 1200 Infinity standard autosampler SL

(G1329B).

Type Specification

Pressure Operating range 0 – 60 MPa (0 – 600 bar, 0 – 8850 psi)

GLP features Early maintenance feedback (EMF), electronic records of

maintenance and errors

Communications Controller-area network (CAN). GPIB (IEEE-448), RS232C,

APG-remote standard, optional four external contact closures and
BCD vial number output

Safety features Leak detection and safe leak handling, low voltages in maintenance
areas, error detection and display

Injection range 0.1 – 100 µl in 0.1 µl increments (recommended 1 µl increments) Up

to 15 00 µl with multiple draw (hardware modification required)

Replicate injections 1 – 99 from one vial

Precision Typically < 0.25% RSD of peak areas from 5 – 100 µl, Typically
< 1% RSD of peak areas from 1 – 5 µl,

Minimum sample volume 1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in

300 µl microvial

Carryover Typically < 0.1%, < 0.05% with external needle cleaning

Sample viscosity range 0.2 – 50 cp

Sample capacity 100 × 2-ml vials in 1 tray

40 × 2-ml vials in ½ tray
15 × 6-ml vials in ½ tray (Agilent vials only)

Injection cycle time 50 s for draw speed 200 µl/min, ejection speed 200 µl/min, injection
volume 5 µl

30
 Site Requirements and Specifications 2
Performance Specifications

Table 8 Performance Specifications Agilent 1260 Infinity Preparative Autosampler

(G2260A)

Type Specification

Pressure Operating range 0 – 40 MPa (0 – 400 bar, 0 – 5800psi)

GLP features Early maintenance feedback (EMF), electronic records of

maintenance and errors

Communications Controller-area network (CAN). GPIB (IEEE-448), RS232C,

APG-remote standard, optional four external contact closures and
BCD vial number output

Safety features Leak detection and safe leak handling, low voltages in maintenance
areas, error detection and display

Injection range 0.1 – 900 µl in 0.1 µl increments (recommended 1 µl increments)

Up to 1800 µl with multiple draw (hardware modification required)
Up to 5000 µl with multiple draw (hardware modification required)

Replicate injections 1 – 99 from one vial

Precision Typically < 0.5% RSD of peak areas from 5 – 2000 µl, Typically
< 1% RSD of peak areas from 2000 – 5000 µl, Typically < 3% RSD of
peak areas from 1 – 5 µl

Minimum sample volume 1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in

300 µl microvial

Sample viscosity range 0.2 – 50 cp

Sample capacity 100 × 2-ml vials in 1 tray

15 × 6-ml vials in ½ tray (Agilent vials only)

Injection cycle time Typically 50 s, depending on draw speed and injection volume

31
2 Site Requirements and Specifications
Performance Specifications

32
3
Installing the Autosampler
Unpacking the Autosampler 34
Damaged Packaging 34
Delivery Checklist 34
Optimizing the Stack Configuration 37
Installing the Autosampler 41
Installing the Thermostatted Autosampler 44
Flow Connections 48
Installing the Sample Tray 50
Transporting the Autosampler 51

Agilent Technologies 33
3 Installing the Autosampler
Unpacking the Autosampler

Unpacking the Autosampler

Mechanical damage of the autosampler

CAUTION
If the transport assembly is not parked, the autosampler could be damaged due to
excessive shock of the shipping container during transport.
➔ Always park the transport assembly before shipment (see “Transporting the
Autosampler” on page 51).

Damaged Packaging
Upon receipt of your autosampler, inspect the shipping containers for any
signs of damage. If the containers or cushioning material are damaged, save
them until the contents have been checked for completeness and the
autosampler has been checked mechanically and electrically. If the shipping
container or cushioning material is damaged, notify the carrier and save the
shipping material for the carriers inspection.

Delivery Checklist
Ensure all parts and materials have been delivered with the autosampler. The
instrument box contains the instrument and an Accessory kit. A separate box
contains the reference manual and the power cable.
In Table 9 on page 35 and Table 10 on page 36 are listed the content of each
accessory kit.
Please report missing or damaged parts to your local Agilent Technologies
sales and service office.

34
 Installing the Autosampler 3
Unpacking the Autosampler

Table 9 G1329A/G1329B - Standard Autosampler Accessory Kit Contents G1329-68725

Description Part Number

Tubing assembly 5063-6527

CAN cable, 1 m long 5181-1519

Screw cap vials, clear 100/pk 5182-0714

Blue screw caps 100/pk 5182-0717

Label halftray 5989-3890

Vial instruction sheet no PN

Wrenches 1/4 - 5/16 inch 8710-0510

Rheotool socket wrench 1/4 inch 8710-2391

Hex key 4 mm, 15 cm long, T-handle 8710-2392

Hex key 9/64 inch, 15 cm long, T- handle 8710-2394

Hex key 2.5 mm, 15 cm long, straight handle 8710-2412

Finger caps (x3)1 5063-6506

Front door cooled autosampler G1329-40301

Air channel adapter G1329-43200

Cover insulation no PN

Capillary 0.17 mm, 900 mm G1329-87300

Capillary heat exchanger 01090-87306

Note for Agilent 1200 Infinity Autosampler door upgrade no PN

1 Reorder gives pack of 15

35
3 Installing the Autosampler
Unpacking the Autosampler

Table 10 G2260A - Preparative Autosampler Accessory Kit Contents G2260-68705

Description Part Number

Tubing assembly 5063-6527

Filter promo kit 5064-8240

CAN cable, 1 m long 5181-1519

Screw cap vials, clear 100/pk 5182-0714

Blue screw caps 100/pk 5182-0717

Label halftray 5989-3890

Wrenches 1/4 - 5/16 inch 8710-0510

Rheotool socket wrench 1/4 inch 8710-2391

Hex key 4 mm, 15 cm long, T-handle 8710-2392

Hex key 9/64 inch, 15 cm long, T- handle 8710-2394

Hex key 2.5 mm, 15 cm long, straight handle 8710-2412

Finger caps x3 (reorder gives pack of 15) 5063-6506

Front door cooled autosampler G1329-40301

Air channel adapter G1329-43200

Tray for 15 x 6 ml vials (x2) G1313-44513

Union, loop extension 5022-2133

Seat extension capillary (500 µl) G1313-87307

Seat extension capillary (1500 µl) G1313-87308

Sampler - Column capillary G2260-87300

36
 Installing the Autosampler 3
Optimizing the Stack Configuration

Optimizing the Stack Configuration

If your autosampler is part of a system, you can ensure optimum performance

by installing the autosampler in the stack in the position shown in Figure 9 on
page 37 and Figure 10 on page 38. Figure 11 on page 39 and Figure 12 on
page 40 show the configuration recommended for a thermostatted
autosampler. These configurations optimize the system flow path, ensuring
minimum delay volume.

HdakZciXVW^cZi

KVXjjbYZ\VhhZg

Ejbe
>chiVciE^adi

6jidhVbeaZgdg
EgZeVgVi^kZ
6jidhVbeaZg

8dajbcXdbeVgibZci

9ZiZXidg

Figure 9 Recommended Stack Configuration for an Autosampler (Front View)

37
3 Installing the Autosampler
Optimizing the Stack Configuration

GZbdiZXVWaZ

68edlZg
86C7jhXVWaZid
>chiVciE^adi

86C7jhXVWaZ

A6CidA8
6cVad\h^\cVaid 8]ZbHiVi^dc
gZXdgYZg

Figure 10 Recommended Stack Configuration for an Autosampler (Rear View)

38
 Installing the Autosampler 3
Optimizing the Stack Configuration

9ZiZXidg >chiVciE^adi

8dajbc
8dbeVgibZci

HdakZci
HiVcYVgYdg 8VW^cZi
EgZeVgVi^kZ
6jidhVbeaZg 9Z\VhhZg

6AHI]ZgbdhiVi Ejbe

Figure 11 Recommended Stack Configuration for a thermostatted ALS (Front View)

39
3 Installing the Autosampler
Optimizing the Stack Configuration

A6CidA88]ZbHiVi^dc

6cVad\h^\cVaidgZXdgYZg

6jidhVbeaZg"I]ZgbdhiViXVWaZ

GZbdiZXVWaZ

6cVad\h^\cVa
idgZXdgYZg

86CWjhXVWaZ

Figure 12 Recommended Stack Configuration for a thermostatted ALS (Rear View)

40
 Installing the Autosampler 3
Installing the Autosampler

Installing the Autosampler

Parts required # Description

1 Sampler
1 Power cord, for the other cables see below and “Cable Overview” on page 120
1 Control Software (ChemStation, EZChrom, OL, etc.) and/or Control Module G1323B

Preparations • Locate bench space

• Provide power connection
• Unpack the Sampler

Module is partially energized when switched off, as long as the power cord is
WA R N I N G
plugged in.
Risk of stroke and other personal injury. Repair work at the module can lead to
personal injuries, e. g. shock hazard, when the module cover is opened and the
instrument is connected to power.
➔ Never perform any adjustment, maintenance or repair of the module with the top
cover removed and with the power cord plugged in.
➔ The security lever at the power input socket prevents that the module cover is taken
off when line power is still connected. Never plug the power line back in when cover
is removed.

Personal injury
WA R N I N G
To avoid personal injury, keep fingers away from the needle area during autosampler
operation.
➔ Do not bend the safety flap away from its position, or attempt to remove the safety
cover (see Figure 13 on page 42).
➔ Do not attempt to insert or remove a vial from the gripper when the gripper is
positioned below the needle.

41
3 Installing the Autosampler
Installing the Autosampler

“Defective on arrival” problems

CAUTION
If there are signs of damage to the autosampler, please do not attempt to install the
autosampler. Inspection by Agilent is required to evaluate if the instrument is in good
condition or damaged.
➔ Notify your Agilent sales and service office about the damage.
➔ An Agilent service representative will inspect the instrument at your site and
initiate appropriate actions.

1 Install the LAN interface board in the sampler (if required).

HV[ZinXdkZg

HV[Zin[aVe

Figure 13 Safety Flap

2 Remove the adhesive tape which covers the front door.

3 Remove the front door and remove the transport protection foam.
4 Place the Autosampler on the bench or in the stack as recommended in
“Optimizing the Stack Configuration” on page 37.
5 Ensure the power switch at the front of the Autosampler is OFF.
6 Connect the power cable to the power connector at the rear of the sampler.
7 Connect the CAN cable to the other Agilent 1200 Infinity modules.
8 If an Agilent Chemstation is the controller, connect either
• The GPIB cable to the detector

42
 Installing the Autosampler 3
Installing the Autosampler

• The LAN connector to the LAN interface

9 Connect the APG remote cable (optional) for non Agilent 1200 Infinity
instruments.
10 Turn ON power by pushing the button at the lower left hand side of the
sampler.
K^VacjbWZgdjieji
86CXVWaZ
idegZk^djhbdYjaZ

GZbdiZ
GH'('8
86C"Wjh
GZaVnXdciVXih
<E>7
Figure 14 Cable Connections

If the front cover is not installed the autosampler is in a not ready condition and operation is
NOTE inhibited.

The sampler is turned ON when the line power switch is pressed and the green indicator
NOTE lamp is illuminated. The detector is turned OFF when the line power switch is protruding
and the green light is OFF.

43
3 Installing the Autosampler
Installing the Thermostatted Autosampler

Installing the Thermostatted Autosampler

Parts required # Description

1 Sampler and Thermostat
1 Power cord, for the other cables see below and “Cable Overview” on page 120
1 Control Software (ChemStation, EZChrom, OL, etc.) and/or Control Module G1323B.

Preparations • Locate bench space

• Provide power connection
• Unpack the Sampler and the Thermostat

Module is partially energized when switched off, as long as the power cord is
WA R N I N G
plugged in.
Risk of stroke and other personal injury. Repair work at the module can lead to
personal injuries, e. g. shock hazard, when the module cover is opened and the
instrument is connected to power.
➔ Never perform any adjustment, maintenance or repair of the module with the top
cover removed and with the power cord plugged in.
➔ The security lever at the power input socket prevents that the module cover is taken
off when line power is still connected. Never plug the power line back in when cover
is removed.

Damaged electronics
WA R N I N G
Disconnecting or reconnecting the autosampler to ALS thermostat cable when the
power cords are connected to either of the two modules will damage the electronics
of the modules.
➔ Make sure the power cords are unplugged before disconnecting or reconnecting the
autosampler to ALS thermostat cable.

44
 Installing the Autosampler 3
Installing the Thermostatted Autosampler

Personal injury
WA R N I N G
To avoid personal injury, keep fingers away from the needle area during
Autosampler operation.
➔ Do not attempt to insert or remove a vial or a plate when the needle is positioned.

Damage through condensation

WA R N I N G
If the condensation tube is located in liquid the condensed water cannot flow out of
the tube and the outlet is blocked. Any further condensation will then remain in the
instrument. This may damage the instruments electronics.
➔ Make sure that the condensation tube is always above the liquid level in the vessel.

1 Place the Thermostat on the bench.

2 Remove the front cover and route the condensation drain tube to the waste.

8dcYZchVi^dcYgV^cijWZ

LVhiZWdiiaZ

Figure 15 Condensation Leak outlet

3 Remove the adhesive tape which covers the front door.

4 Remove the front door and remove the transport protection foam.
5 Place the Autosampler on top of the Thermostat. Make sure that the
Autosampler is correctly engaged in the Thermostat locks.

45
3 Installing the Autosampler
Installing the Thermostatted Autosampler

6 Place the air channel adapter into the autosampler tray base. Make sure the
adapter is fully pressed down. This assures that the cold airstream from the
Thermostat is correctly guided to the tray area of the Autosampler.

6^gX]VccZaVYVeiZg

Figure 16 Air channel adapter

7 Re-install the tray

8 Ensure the power switch on the front of the Autosampler is OFF and the
power cables are disconnected.
9 Connect the cable between the Autosampler and the Thermostat, see
Figure 17 on page 47.
10 Connect the power cables to the power connectors.
11 Connect the CAN cable to the other Agilent 1200 Infinity modules.
12 If an Agilent ChemStation is the controller, connect either
• The GPIB cable to the detector
• The LAN connector to the LAN interface
13 Connect the APG remote cable (optional) for non Agilent 1200 Infinity
instruments.
14 Turn ON power by pushing the button at the lower left hand side of the
sampler.

The sampler is turned ON when the line power switch is pressed and the green indicator
NOTE lamp is illuminated. The detector is turned OFF when the line power switch is protruding
and the green light is OFF.

46
 Installing the Autosampler 3
Installing the Thermostatted Autosampler

GZaVnXdciVXih

K^VacjbWZgdjieji

I]ZgbdhiVi"
6jidhVbeaZg
XVWaZ

GZbdiZ
GH'('8
86C"Wjh

<E>7

Figure 17 Cable Connections

47
3 Installing the Autosampler
Flow Connections

Flow Connections

Parts required # Description

1 Parts from the Accessory kit

Preparations • Sampler is installed in the LC system

Toxic and hazardous solvents

WA R N I N G
The handling of solvents and reagents can hold health risks.
➔ When opening capillary or tube fittings solvents may leak out.
➔ Please observe appropriate safety procedures (for example, goggles, safety gloves
and protective clothing) as described in the material handling and safety data sheet
supplied by the solvent vendor, especially when toxic or hazardous solvents are
used.

1 Connect the pump outlet capillary to port 1 of the injection valve.

2 Connect column-compartment inlet capillary to port 6 of the injection
valve.
3 Connect the corrugated waste tube to the solvent waste from the leak plane.
4 Ensure that the waste tube is positioned inside the leak channel.

Do not extend the waste capillary of the autosampler. The siphoning effect might empty the
NOTE complete seat capillary introducing air into the system.

48
 Installing the Autosampler 3
Flow Connections

EjbedjiaZiXVe^aaVgn

LVhiZijWZ^c
AZV`X]VccZa

HdakZcilVhiZ[dgXdggj\ViZYlVhiZijWZ
8dajbcXdbeVgibZci^caZiXVe^aaVgn
Figure 18 Hydraulic Connections

49
3 Installing the Autosampler
Installing the Sample Tray

Installing the Sample Tray

1 Open the front door.

2 Load the sample tray with sample vials as required.
3 Slide the sample tray into the autosampler so that the rear of the sample
tray is seated firmly against the rear of the sample-tray area.
4 Press the front of the sample tray down to secure the tray in the
autosampler.

If the thermostatted autosampler tray pops out of position the air channel adapter is not
NOTE inserted correctly.

Figure 19 Installing the Sample Tray

50
 Installing the Autosampler 3
Transporting the Autosampler

Transporting the Autosampler

When moving the autosampler around the laboratory, no special precautions

are needed. However, if the autosampler needs to be shipped to another
location via carrier, ensure:
• The transport assembly is parked;
• The vial tray is secured.
If the autosampler is to be shipped to another location, the transport assembly
must be moved to the park position to prevent mechanical damage should the
shipping container be subjected to excessive shock. Also, ensure the vial tray
is secured in place with suitable packaging, otherwise the tray may become
loose and damage internal components.

51
3 Installing the Autosampler
Transporting the Autosampler

52
4
Using the Autosampler
Solvent Information 54
Sample Trays 56
Choice of Vials and Caps 58

Agilent Technologies 53
4 Using the Autosampler
Solvent Information

Solvent Information

Observe the following recommendations on the use of solvents.

Flow Cell
Avoid the use of alkaline solutions (pH > 9.5) which can attack quartz and thus
impair the optical properties of the flow cell.
Prevent any crystallization of buffer solutions. This will lead into a
blockage/damage of the flow cell.
If the flow cell is transported while temperatures are below 5 °C, it must be
assured that the cell is filled with alcohol.
Aqueous solvents in the flow cell can built up algae. Therefore do not leave
aqueous solvents sitting in the flow cell. Add small % of organic solvents (e.g.
Acetonitrile or Methanol ~5%).

Solvents
Brown glass ware can avoid growth of algae.
Always filter solvents, small particles can permanently block the capillaries.
Avoid the use of the following steel-corrosive solvents:
• Solutions of alkali halides and their respective acids (for example, lithium
iodide, potassium chloride, and so on).
• High concentrations of inorganic acids like nitric acid, sulfuric acid
especially at higher temperatures (replace, if your chromatography method
allows, by phosphoric acid or phosphate buffer which are less corrosive
against stainless steel).
• Halogenated solvents or mixtures which form radicals and/or acids, for
example:
2CHCl3 + O2 → 2COCl2 + 2HCl
This reaction, in which stainless steel probably acts as a catalyst, occurs
quickly with dried chloroform if the drying process removes the stabilizing
alcohol.

54
 Using the Autosampler 4
Solvent Information

• Chromatographic grade ethers, which can contain peroxides (for example,

THF, dioxane, di-isopropylether). Such ethers should be filtered through
dry aluminium oxide which adsorbs the peroxides.
• Solutions of organic acids (acetic acid, formic acid, and so on) in organic
solvents. For example, a 1% solution of acetic acid in methanol will attack
steel.
• Solutions containing strong complexing agents (for example, EDTA,
ethylene diamine tetra-acetic acid).
• Mixtures of carbon tetrachloride with 2-propanol or THF.

55
4 Using the Autosampler
Sample Trays

Sample Trays

Supported trays for the different Autosampler

Table 11 Supported trays for the Autosampler (G1329A/ G2260A)

Description Part Number

Tray for 100 x 2 ml vials G1313-44510

Halftray for 15 x 6 ml vials G1313-44513

Halftray for 40 x 2 ml vials G1313-44512

Thermostattable Tray for 100 x 2 ml vials G1329-60011

Halftray for 15 x 6 ml vials (for G2260A only1) G1313-44513

1 This tray is not recommended when using a thermostat

Half-Tray Combinations
Half-trays can be installed in any combination enabling both 2 ml-and
6 ml-vials to be used simultaneously.

56
 Using the Autosampler 4
Sample Trays

Numbering of Vial Positions

The standard 100-vial tray has vial positions 1 to 100. However, when using
two half-trays, the numbering convention is slightly different. The vial
positions of the right-hand half tray begin at position 101 as follows:
Left-hand 40-position tray: 1 - 40
Left-hand 15-position tray: 1–15
Right-hand 40-position tray: 101–140
Right-hand 15-position tray: 101–115

Edh^i^dc&

AZ[i]VcY]Va[igVn

Edh^i^dc&%&

G^\]i]VcY]Va[igVn

Figure 20 Numbering of Tray Positions

57
4 Using the Autosampler
Choice of Vials and Caps

Choice of Vials and Caps

List of Compatible Vials and Caps

For reliable operation vials used with the Agilent 1200 Infinity autosampler
must not have tapered shoulders or caps that are wider than the body of the
vial. The vials in Table 12 on page 58, Table 13 on page 58 and Table 14 on
page 59 and caps in Table 15 on page 59, Table 16 on page 59 and Table 17 on
page 60 (shown with their Part numbers) have been successfully tested using a
minimum of 15,000 injections with the Agilent 1200 Infinity autosampler.

Table 12 Crimp Top Vials

Description Volume (ml) 100/Pack 1000/Pack 100/Pack

(silanized)

Clear glass 2 5181-3375 5183-4491

Clear glass, 2 5182-0543 5183-4492 5183-4494

write-on spot

Amber glass, 2 5182-3376 5183-4493 5183-4495

write-on spot

Polypropylene, 1 5182-0567 5183-4496

wide opening

Polypropylene, 0.3 9301-0978

wide opening

Table 13 Snap Top Vials

Description Volume (ml) 100/Pack 1000/Pack 100/Pack

(silanized)

Clear glass 2 5182-0544 5183-4504 5183-4507

Clear glass, 2 5182-0546 5183-4505 5183-4508

write-on spot

Amber glass, 2 5182-0545 5183-4506 5183-4509

write-on spot

58
 Using the Autosampler 4
Choice of Vials and Caps

Table 14 Screw Top Vials

Description Volume (ml) 100/Pack 1000/Pack 100/Pack

(silanized)

Clear glass 2 5182-0714 5183-2067 5183-2070

Clear glass, 2 5182-0715 5183-2068 5183-2071

write-on spot

Amber glass, 2 5182-0716 5183-2069 5183-2072

write-on spot

Table 15 Crimp Caps

Description Septa 100/Pack

Silver aluminum Clear PTFE/red rubber 5181-1210

Silver aluminum Clear PTFE/red rubber 5183-4498 (1000/Pack)

Blue aluminum Clear PTFE/red rubber 5181-1215

Green aluminum Clear PTFE/red rubber 5181-1216

Red aluminum Clear PTFE/red rubber 5181-1217

Table 16 Snap Caps

Description Septa 100/Pack

Clear polypropylene Clear PTFE/red rubber 5182-0550

Blue polypropylene Clear PTFE/red rubber 5182-3458

Green polypropylene Clear PTFE/red rubber 5182-3457

Red polypropylene Clear PTFE/red rubber 5182-3459

59
4 Using the Autosampler
Choice of Vials and Caps

Table 17 Screw Caps

Description Septa 100/Pack

Blue polypropylene Clear PTFE/red rubber 5182-0717

Green polypropylene Clear PTFE/red rubber 5182-0718

Red polypropylene Clear PTFE/red rubber 5182-0719

Blue polypropylene Clear PTFE/silicone 5182-0720

Green polypropylene Clear PTFE/silicone 5182-0721

Red polypropylene Clear PTFE/silicone 5182-0722

60
5
Optimizing Performance
Optimization for Lowest Carry-over 62
Using the Automated Needle Wash 63
Using an Injector Program 64
General Recommendation to Lowest Carry-over 65
Fast Injection Cycle and Low Delay Volume 66
Overlapped Injection Mode 66
General Recommendations for Fast Injection Cycle Times 67
Precise Injection Volume 68
Draw and Eject Speed 68
Choice of Rotor Seal 70

Agilent Technologies 61
5 Optimizing Performance
Optimization for Lowest Carry-over

Optimization for Lowest Carry-over

Several parts of an injection system can contribute to carry-over:

• needle outside
• needle inside
• needle seat
• sample loop
• seat capillary
• injection valve
The autosampler continuous flow-through design ensures that sample loop,
needle inside, seat capillary, and the mainpass of the injection valve is always
in the flow line. These parts are continuously flushed during an isocratic and
also during a gradient analysis. The residual amount of sample remaining on
the outside of the needle after injection may contribute to carry-over in some
instances. When using small injection volumes or when injecting samples of
low concentration immediately after samples of high concentration, carry-over
may become noticeable. Using the automated needle wash enables the
carry-over to be minimized and prevents also contamination of the needle
seat.

62
 Optimizing Performance 5
Optimization for Lowest Carry-over

Using the Automated Needle Wash

The automated needle wash can be programmed either as “injection with
needle wash” or the needle wash can be included into the injector program.
When the automated needle wash is used, the needle is moved into a wash vial
after the sample is drawn. By washing the needle after drawing a sample, the
sample is removed from the surface of the needle immediately.

Uncapped Wash Vial

For best results, the wash vial should contain solvent in which the sample
components are soluble, and the vial should not be capped. If the wash vial is
capped, small amounts of sample remain on the surface of the septum, which
may be carried on the needle to the next sample.

Injector Program with Needle Wash

The injector program includes the command NEEDLE WASH. When this
command is included in the injector program, the needle is lowered once into
the specified wash vial before injection.
For example:
1 DRAW 5 µl
2 NEEDLE WASH vial 7
3 INJECT
Line 1 draws 5 µl from the current sample vial. Line 2 moves the needle to vial
7. Line 3 injects the sample (valve switches to main pass).

63
5 Optimizing Performance
Optimization for Lowest Carry-over

Using an Injector Program

The process is based on a program that switches the bypass grove of the
injection valve into the flow line for cleaning. This switching event is
performed at the end of the equilibration time to ensure that the bypass grove
is filled with the start concentration of the mobile phase. Otherwise the
separation could be influenced, especially if microbore columns are used.

For example:
Outside wash of needle in vial 7 before injection
Injector program:
Draw x.x (y) µl from sample
NEEDLE WASH vial 7
Inject
Wait (equilibration time - see text above)
Valve bypass
Wait 0.2 min
Valve mainpass
Valve bypass
Valve mainpass

Overlapped injection together with additional injection valve switching is not possible.
NOTE

64
 Optimizing Performance 5
Optimization for Lowest Carry-over

General Recommendation to Lowest Carry-over

• For samples where needle outside cannot be cleaned sufficiently with water
or alcohol use wash vials with an appropriate solvent. Using an injector
program and several wash vials can be used for cleaning.
In case the needle seat has got contaminated and carry-over is significantly
higher than expected, the following procedure can be used to clean the needle
seat:
• Go to MORE INJECTOR and set needle to home position.
• Pipette an appropriate solvent on to the needle seat. The solvent should be
able to dissolve the contamination. If this is not known use 2 or 3 solvents
of different polarity. Use several milliliters to clean the seat.
• Clean the needle seat with a tissue and remove all liquid from it.
• RESET the injector.

65
5 Optimizing Performance
Fast Injection Cycle and Low Delay Volume

Fast Injection Cycle and Low Delay Volume

Short injection cycle times for high sample througput is one of the most
important requirements in analytical laboratories. In order to shorten cycle
times, you can:
• shorten the column length
• use high flow rates
• apply a steep gradient
Having optimized these parameters, further reduction of cycle times can be
obtained using the overlapped injection mode.

Overlapped Injection Mode

In this process, as soon as the sample has reached the column, the injection
valve is switched back to bypass and the next injection cycle starts but waits
with switching to mainpass until the actual run is finished. You gain the
sample preparation time when using this process.
Switching the valve into the bypass position reduces the system delay volume,
the mobile phase is directed to the column without passing sample loop,
needle and needle seat capillary. This can help to have faster cycle times
especially if low flow rates have to be used like it is mandatory in narrow bore
and micro bore HPLC.

Having the valve in bypass position can increase the carry-over in the system.
NOTE

The injection cycle times also depend on the injection volume. In identically
standard condition, injecting 100 µl instead of 1 µl, increase the injection time
by approximately 8 sec. In this case and if the viscosity of the sample allows it,
the draw and eject speed of the injection system has to be increased.

66
 Optimizing Performance 5
Fast Injection Cycle and Low Delay Volume

For the last injection of the sequence with overlapped injections it has to be considered
NOTE that for this run the injection valve is not switched as for the previous runs and
consequently the injector delay volume is not bypassed. This means the retention times are
prolonged for the last run. Especially at low flow rates this can lead to retention time
changes which are too big for the actual calibration table. To overcome this it is
recommended to add an additional “blank” injection as last injection to the sequence.

General Recommendations for Fast Injection Cycle Times

As described in this section, the first step to provide short cycle times are
optimizing the chromatographic conditions. If this is done the autosampler
parameter should be set to:
• Overlapped injection mode
• Increase of draw and eject speed for large injection volumes
• Add at last run a blank, if overlapped injection is used
To reduce the injection time, the detector balance has to be set to OFF.

67
5 Optimizing Performance
Precise Injection Volume

Precise Injection Volume

Injection Volumes Less Than 2 µl

When the injection valve switches to the BYPASS position, the mobile phase in
the sample loop is depressurized. When the syringe begins drawing sample,
the pressureof the mobile phase is decreased further. If the mobile phase is
not degassed adequately, small gas bubbles may form in the sample loop
during the injection sequence. When using injection volumes < 2 µl, these gas
bubbles may affect the injection-volume precision. For best injection-volume
precision with injection volumes < 2 µl, use of the Agilent 1200 Infinity
degasser is recommended to ensure the mobile phase is adequately degassed.
Also, using the automated needle wash (see “Optimization for Lowest
Carry-over” on page 62) between injections reduces carry-over to a minimum,
further improving the injection volume precision.

Draw and Eject Speed

Draw Speed
The speed at which the metering unit draws sample out of the vial may have an
influence on the injection volume precision when using viscous samples. If the
draw speed is too high, air bubbles may form in the sample plug, affecting
precision. The default draw speed is 200 µl/min for the autosampler and
1000 µl/min for the preparative autosampler. This speed is suitable for the
majority of applications, however, when using viscous samples, set the draw
speed to lower speed for optimum results. A “DRAW” statement in an injector
program also uses the draw speed setting which is configured for the
autosampler.

68
 Optimizing Performance 5
Precise Injection Volume

Eject Speed
The default eject speed setting is 200 µl/min for the standard autosampler and
1000 µl/min for the preparative autosampler. When using large injection
volumes, setting the eject speed to a higher value speeds up the injection cycle
by shortening the time the metering unit requires to eject solvent at the
beginning of the injection cycle (when the plunger returns to the home
position).
An “EJECT” statement in an injector program also uses the eject speed setting
which is configured for the autosampler. A faster eject speed shortens the time
required to run the injector program. When using viscous samples, a high eject
speed should be avoided.

69
5 Optimizing Performance
Choice of Rotor Seal

Choice of Rotor Seal

Vespel™ Seal (for standard valves only)

The standard seal has sealing material made of Vespel. Vespel is suitable for
applications using mobile phases within the pH range of 2.3 to 9.5, which is
suitable for the majority of applications. However, for applications using
mobile phases with pH below 2.3 or above 9.5, the Vespel seal may degrade
faster, leading to reduced seal lifetime.

Tefzel™ Seal (for standard valve only)

For mobile phases with pH below 2.3 or above 9.5, or for conditions where the
lifetime of the Vespel seal is drastically reduced, a seal made of Tefzel is
available. Tefzel is more resistant than Vespel to extremes of pH, however, is a
slightly softer material. Under normal conditions, the expected lifetime of the
Tefzel seal is shorter than the Vespel seal, however, Tefzel may have the longer
lifetime under more extreme mobile phase conditions.

PEEK Seal (for preparative injection valve only)

The preparative injection valve has a sealing material made of PEEK. This
material has high chemical resistance and versatility. It is suitable for
application using mobile phases within a pH between 1 and 14.
This seal is also used for the G1329B module.

Strong oxidizing acids such as concentrated nitric and sulfuric acids are not compatible
NOTE with PEEK.

70
6
Troubleshooting and Diagnostics
Agilent Lab Monitor & Diagnostic Software 72
Overview of the Sampler’s Indicators and Test Functions 73

Agilent Technologies 71
6 Troubleshooting and Diagnostics
Agilent Lab Monitor & Diagnostic Software

Agilent Lab Monitor & Diagnostic Software

The Agilent Lab Advisor Software is a standalone product that can be used
with or without data system. Agilent Lab Advisor helps to manage the lab for
high quality chromatographic results and can monitor in real time a single
Agilent LC or all the Agilent GCs and LCs configured on the lab intranet.
Agilent Lab Advisor provides diagnostic capabilities for all Agilent 1200
Infinity HPLC modules. This includes tests and calibrations procedures as well
as the different injector steps to perform all the maintenance routines.
Agilent Lab Advisor also allows users to monitor the status of their LC
instruments. The Early Maintenance Feedback (EMF) feature helps to carry
out preventive maintenance. In addition, users can generate a status report for
each individual LC instrument. The tests and diagnostic features as provided
by the Agilent Lab Advisor Software may differ from the descriptions in this
manual. For details refer to the Agilent Lab Advisor help files.
This manual provides lists with the names of Error Messages, Not Ready
messages, and other common issues.

72
 Troubleshooting and Diagnostics 6
Overview of the Sampler’s Indicators and Test Functions

Overview of the Sampler’s Indicators and Test Functions

Status Indicators
The autosamplers are provided with two status indicators which indicate the
operational state (prerun, run, and error states) of the instrument. The status
indicators provide a quick visual check of the operation of the autosampler .

Error Messages
In the event of an electronic, mechanical or hydraulic failure, the instrument
generates an error message in the user interface. For details on error
messages and error handling, please refer to the Agilent Lab Monitor &
Diagnostic Software.

Maintenance Functions
The maintenance functions position the needle arm, gripper assembly, and
metering device for easy access when doing maintenance.

Step Commands
The step functions provide the possibility to execute each step of the sampling
sequence individually. The step functions are used primarily for
troubleshooting, and for verification of correct autosampler operation after
repair.

73
6 Troubleshooting and Diagnostics
Overview of the Sampler’s Indicators and Test Functions

74
7
Maintenance
Introduction into Maintenance and Repair 76
Simple Repairs 76
Exchanging Internal Parts 76
Safety Flap, Flex Board 76
Transport Assembly Parts 76
Updating the Firmware 77
Warnings and Cautions 78
Using the ESD Strap 78
Cleaning the module 79
Overview of Main Repair Procedures 80
Early Maintenance Feedback (EMF) 82
EMF Counters 82
Using the EMF Counters 83
Maintenance Functions 84
Simple Repairs 85
Needle Assembly 86
Needle-Seat Assembly 89
Stator Face 91
Rotor Seal 94
Metering Seal and Plunger 98
Gripper Arm 102
Interface Board 104

Agilent Technologies 75
7 Maintenance
Introduction into Maintenance and Repair

Introduction into Maintenance and Repair

Simple Repairs
The autosampler is designed for easy repair. The most frequent repairs such as
change and needle assembly change can be done from the front of the
instrument with the instrument in place in the system stack. These repairs are
described in Table 18 on page 85.

Exchanging Internal Parts

Some repairs may require exchange of defective internal parts. Exchange of
these parts requires removing the autosampler from the stack, removing the
covers, and disassembling the autosampler.

Safety Flap, Flex Board

It is strongly recommended that the exchange of the safety flap, and flex board
is done by Agilent-trained service personnel.

Transport Assembly Parts

The adjustment of the motors, and the tension on the drive belts are important
for correct operation of the transport assembly. It is strongly recommended
that exchange of drive belts, and the gripper assembly is done by
Agilent-trained service personnel. There are no other field-replaceable parts
in the transport assembly. If any other component is defective (flex board,
spindles, plastic parts) the complete unit must be exchanged.

76
 Maintenance 7
Introduction into Maintenance and Repair

Updating the Firmware

The Agilent 1200 Infinity LC modules are fitted with FLASH EPROMS. These
EPROMS enable you to update the instrument firmware from the
ChemStation, PCMCIA card, or through the RS232 interface. The firmware
update procedure is described in the on-line user information.

77
7 Maintenance
Introduction into Maintenance and Repair

Warnings and Cautions

Module is partially energized when switched off, as long as the power cord is
WA R N I N G
plugged in.
Repair work at the module can lead to personal injuries, e.g. shock hazard, when the
cover is opened and the module is connected to power.
➔ Make sure that it is always possible to access the power plug.
➔ Remove the power cable from the instrument before opening the cover.
➔ Do not connect the power cable to the Instrument while the covers are removed.

Using the ESD Strap

Electronic boards are sensitive to electronic discharge (ESD). In order to
prevent damage, always use an ESD kit when handling electronic boards and
components. Use the ESD kit according to recommendations given by the
supplier.

78
 Maintenance 7
Introduction into Maintenance and Repair

Cleaning the module

The module case should be kept clean. Cleaning should be done with a soft
cloth slightly dampened with water or a solution of water and mild detergent.
Do not use an excessively damp cloth as liquid may drip into the module.

Liquid dripping into the electronic compartment of your module.

WA R N I N G
Liquid in the module electronics can cause shock hazard and damage the module.
➔ Do not use an exessively damp cloth during cleaning.
➔ Drain all solvent lines before opening any fittings.

79
7 Maintenance
Overview of Main Repair Procedures

Overview of Main Repair Procedures

'
(

,
&
Figure 21 Main Assemblies

80
 Maintenance 7
Overview of Main Repair Procedures

1 Power supply

2 ASM board

3 Transport assembly

4 Metering seal, “Metering Seal and Plunger” on page 98

5 Needle, “Needle Assembly” on page 86

6 Rotor seal, “Rotor Seal” on page 94

7 Needle seat, “Needle-Seat Assembly” on page 89

81
7 Maintenance
Early Maintenance Feedback (EMF)

Early Maintenance Feedback (EMF)

Maintenance requires the exchange of components in the flow path which are
subject to mechanical wear or stress. Ideally, the frequency at which
components are exchanged should be based on the intensity of usage of the
instrument and the analytical conditions, and not on a predefined time
interval. The early maintenance feedback (EMF) feature monitors the usage of
specific components in the instrument, and provides feedback when the
user-setable limits have been exceeded. The visual feedback in the user
interface provides an indication that maintenance procedures should be
scheduled.

EMF Counters
The autosampler provides two EMF counters. Each counter increments with
autosampler use, and can be assigned a maximum limit which provides visual
feedback in the user interface when the limit is exceeded. Each counter can be
reset to zero after maintenance has been done. The autosampler provides the
following EMF counters:

Injection valve counter

This counter display the total number of switches of the injection valve since
the last reset of the counter.

Needle Movements Counter

This counter displays the total number of movements of the needle into the
seat since the last reset of the counter.

82
 Maintenance 7
Early Maintenance Feedback (EMF)

Using the EMF Counters

The user-setable EMF limits for the EMF counters enable the early
maintenance feedback to be adapted to specific user requirements. The wear
of autosampler components is dependent on the analytical conditions,
therefore, the definition of the maximum limits need to be determined based
on the specific operating conditions of the instrument.

Setting the EMF Limits

The setting of the EMF limits must be optimized over one or two maintenance
cycles. Initially, no EMF limit should be set. When instrument performance
indicates maintenance is necessary, make note of the values displayed by the
injection valve and needle movements counters. Enter these values (or values
slightly less than the displayed values) as EMF limits, and then reset the EMF
counters to zero. The next time the EMF counters exceed the new EMF limits,
the EMF flag will be displayed, providing a reminder that maintenance needs
to be scheduled.

83
7 Maintenance
Maintenance Functions

Maintenance Functions

Certain maintenance procedures require the needle arm, metering device, and
gripper assembly to be moved to specific positions to enable easy access to
components. The maintenance functions move these assemblies into the
appropriate maintenance position.

84
 Maintenance 7
Simple Repairs

Simple Repairs

The procedures described in this section can be done with the autosampler in
place in the stack. You will do some of these procedures on a more frequent
basis.

Table 18 Simple Repair Procedures

Procedure Typical Frequency Time Required Notes

Exchanging the needle When needle shows indication of 15 minutes See “Needle Assembly” on page 86
assembly damage or blockage

Exchanging the seat When the seat shows indication of 10 minutes See “Needle-Seat Assembly” on page 89
assembly damage or blockage

Exchanging the rotor After approximately 30000 to 30 minutes See “Rotor Seal” on page 94
seal 40000 injections, or when the
valve performance shows
indication of leakage or wear

Exchanging the When autosampler reproducibility 30 minutes See “Metering Seal and Plunger” on
metering seal indicates seal wear page 98

Exchanging the gripper When the gripper arm is defective 10 minutes See “Gripper Arm” on page 102
arm

85
7 Maintenance
Simple Repairs

Needle Assembly
When When the needle is visibly damaged
When the needle is blocked

Tools required • ¼ inch wrench (supplied in accessory kit)

• 2.5 mm Hex key (supplied in accessory kit)
• A pair of pliers

Parts required # Part number Description

1 G1313-87201 Needle assembly for G1313-87101 or G1313-87103 needle-seat
1 G1329-80001 Needle assembly for G1329-87101 or G1329-87103 needle seat
1 G1313-87202 Needle assembly (900 µl loop cap) for G1313-87101 needle seat
1 G2260-87201 Needle assembly (900 µl loop cap) for G2260-87101 needle-seat

Preparations • Select “Start” in the maintenance function “Change Needle”.

• When the needle is positioned approx.15 mm above the needle seat, remove the front cover.

Personal injury
WA R N I N G
To avoid personal injury, keep fingers away from the needle area during autosampler
operation.
➔ Do not bend the safety flap away from its position, or attempt to insert or remove a
vial from the gripper when the gripper is positioned below the needle.

86
 Maintenance 7
Simple Repairs

1 Select Needle Down until the needle screw is aligned 2 Remove the sample-loop fitting from the needle fitting.
with the hole in the safety cover.

3 Loosen the fixing screw, and lift out the needle. 4 Insert the new needle. Align the needle in the seat, then
tighten the screw firmly.

87
7 Maintenance
Simple Repairs

5 Reconnect the sample-loop fitting to the needle fitting. 6 Use Needle Up to lift the needle to a position
approximately 2mm above the seat.

7 Ensure the needle is aligned with the seat. Next Steps:

8 On completion of this procedure: Install the front cover.

9 Select “End” in the maintenance function “Change
Needle”.

88
 Maintenance 7
Simple Repairs

Needle-Seat Assembly
When When the seat is visibly damaged
When the seat capillary is blocked

Tools required • 1/4 inch wrench (supplied in accessory kit).

• Flat-head screwdriver.

Parts required # Part number Description

1 G1313-87101 Needle-seat assy (0.17 mm i.d 2.3 µl) for G1329A/B
1 G1313-87103 Needle-seat assy (0.12 mm i.d 1.2 µl) for G1329A/B
1 G2260-87101 Needle-seat assy (0.50 mm i.d 20 µl) for G2260A

Preparations • Select “Start” in the maintenance function “Change Needle”.

• Remove the front cover.
• Use the “Needle Up” command in the “Change Needle” function to lift the needle an addition 1
cm.

1 Disconnect the seat-capillary fitting from the injection 2 Use a small flat-head screwdriver to ease out the needle
valve (port 5). seat.

89
7 Maintenance
Simple Repairs

3 Insert the new needle-seat assembly. Press the seat 4 Connect the seat-capillary fitting to port 5 of the injection
firmly into position. valve.

5 Use “Down” to position the needle approximately 2 mm 6 Ensure the needle is aligned with the seat. If required,
above the seat bend the needle slightly until the needle is aligned
correctly.

Next Steps:

7 On completion of this procedure: Install the front cover.

8 Select “End” in the maintenance function “Change Needle”.

90
 Maintenance 7
Simple Repairs

Stator Face
When Poor injection-volume reproducibility
Leaking injection valve

Tools required • 1/4 inch wrench (supplied in accessory kit)

• Hex key, 9/64 inch (supplied in accessory kit)

Parts required # Part number Description

1 0100-1851 Stator face for G1329A
1 No stator face for G1329B (functionality part of stator head).
1 0101-1268 Stator face for G2260A

Preparations • Remove the front cover.

• Remove the leak tubings (if necessary).

Removing the stator head

CAUTION
The stator face is held in place by the stator head. When you remove the stator head,
the stator face can fall out of the valve.
➔ Carefully handle the valve to prevent damage to the stator face

1 Remove all capillary fittings from the injection-valve ports 2 Loosen each fixing bolt two turns at a time. Remove the
. bolts from the head.

91
7 Maintenance
Simple Repairs

3 Remove the stator head and stator face. 4 Place the stator face in place on the stator head. Ensure
the pins on the stator engage in the holes in the stator
head.

5 Install stator head and stator face. Tighten the bolts 6 Reconnect the pump capillaries to the valve ports (see
alternately two turns at a time until the stator head is Figure 18 on page 49).
secure.

92
 Maintenance 7
Simple Repairs

7 Slide the waste tube into the waste holder in the leak tray. 8 On completion of this procedure: Install the front cover.

93
7 Maintenance
Simple Repairs

Rotor Seal
When Poor injection-volume reproducibility
Leaking injection valve

Tools required • 1/4 inch wrench (supplied in accessory kit).

• Hex key, 9/64 inch (supplied in accessory kit).

Parts required # Part number Description

1 0100-1853 Rotor seal (Vespel) for G1329A
1 0100-1849 Rotor seal (Tefzel) for G1329A
1 0101-1416 Rotor seal (PEEK) for G1329B
1 0101-1268 Rotor seal (PEEK) for G2260A

Preparations • Remove front cover.

• Remove the leak tubing (if necessary).

Removing the stator head

CAUTION
The stator face is held in place by the stator head. When you remove the stator head,
the stator face can fall out of the valve.
➔ Carefully handle the valve to prevent damage to the stator face

There is no stator face for G1329B.

NOTE

94
 Maintenance 7
Simple Repairs

1 Remove all capillary fittings from the injection-valve ports. 2 Loosen each fixing bolt two turns at a time. Remove the
bolts from the head.

3 Remove the stator head, stator face and stator ring. 4 Remove the rotor seal and isolation seal.

95
7 Maintenance
Simple Repairs

5 Install the new rotor seal and isolation seal. Ensure the 6 Install the stator ring with the short of the two pins facing
metal spring inside the isolation seal faces towards the towards you at the 12 O’Clock position. Ensure the ring
valve body. sits flat on the valve body.

7 Place the stator face in place on the stator head. 8 Install stator head and stator face. Tighten the bolts
alternately two turns at a time until the stator head is
secure.

96
 Maintenance 7
Simple Repairs

9 Reconnect the pump capillaries to the valve ports (see 10 Slide the waste tube into the waste holder in the leak tray.
Figure 18 on page 49).

11 On completion of this procedure: Install the front cover.

97
7 Maintenance
Simple Repairs

Metering Seal and Plunger

When Poor injection-volume reproducibility
Leaking metering device

Tools required • 1/4 inch wrench (supplied in accessory kit).

• 4 mm hex key (supplied in accessory kit).
• 3 mm hex key (supplied in accessory kit).

Parts required # Part number Description

1 5063-6589 Metering seal (pack of 2) for 100 µl analytical head
1 Metering seal (pack of 1) for 900 µl analytical head
1 5063-6586 Metering plunger for 100 µl analytical head
1 5062-8587 Metering plunger for 900 µl analytical head (only if scratched or contaminated)

Preparations • Select “Start” in the maintenance function “Change piston”.

• Remove the front cover.

1 Remove the two capillaries from the metering-head 2 Remove the two fixing bolts, and pull the head assembly
assembly. away from the sampler. Notice that the closed side of the
metering head faces upwards.

98
 Maintenance 7
Simple Repairs

3 Remove the two fixing bolts from the base of the metering 4 Disassemble the metering head assembly.
head assembly.

5 Use a small screwdriver to carefully remove the seal. 6 Install the new seal. Press the seal firmly into position.
Clean the chamber with lint-free cloth. Ensure all
particular matter is removed.

99
7 Maintenance
Simple Repairs

7 Place the piston guide on top of the seal. 8 Reassemble the metering head assembly. Carefully insert
the plunger into the base. The closed side of the metering
head must be on the same side as the lower one of the
two capillary drillings.

9 Install the fixing bolts. Tighten the bolts securely. 10 Install the metering head assembly in the autosampler.
Ensure the large hole in the metering head is facing
downwards.

100
 Maintenance 7
Simple Repairs

11 Reinstall the capillaries. Next Steps:

12 On completion of this procedure: Install the front cover.

13 Select “End” in the maintenance function “Change
piston” .

101
7 Maintenance
Simple Repairs

Gripper Arm
When Defective gripper arm

Tools required • Straightened paper clip.

Parts required # Part number Description

1 G1313-60010 Gripper assembly

Preparations • Select “Start” in the maintenance function “ChangeGripper”.

• Turn off the power to the autosampler.
• Remove the front cover.

1 Identify the slit below the gripper motor and the gripper 2 Rotate the arm approximately 2.5cm (1 inch) to the left
arm release button. and insert the straightened paper clip into the slit.
Wjiidc

ha^i

102
 Maintenance 7
Simple Repairs

3 Rotate the gripper arm slowly from left to right and apply a 4 Hold the paper clip in place, press the gripper release
gentle pressure to the paper clip. The clip will engage on button and rotate the gripper arm to the right.
an internal catch and the rotation of the arm will be
blocked.

5 The gripper arm will come off. 6 Replace the gripper arm by holding the paper clip in place,
pushing the gripper arm into the holder and rotating the
gripper arm to the left.

Next Steps:

7 On completion of this procedure: Install the front cover.

8 Turn the power to the autosampler ON.

103
7 Maintenance
Simple Repairs

Interface Board
When At installation or when defective.

Tools required • Flat-head screwdriver.

Parts required # Description

1 Interface board.

Electronic boards are static sensitive and should be handled with care so as not to
CAUTION
damage them. Touching electronic boards and components can cause electrostatic
discharge (ESD).
ESD can damage electronic boards and components.
➔ Be sure to hold the board by the edges and do not touch the electrical components.
Always use an ESD protection (for example, an ESD wrist strap) when handling
electronic boards and components.

1 Switch OFF the autosampler at the main power switch.

2 Disconnect cables from the interface board connectors.
3 Loosen the screws. Slide out the interface board from the autosampler.
4 Install the interface board. Secure the screws.
5 Reconnect the cables to the board connectors

HXgZlh

Figure 22 Exchanging the Interface Board

104
8
Parts and Materials for Maintenance
Main Assemblies 106
Analytical-Head Assembly 108
Vial Trays 111
Standard Autosampler Accessory Kit G1329-68725 112
Preparative Autosampler Accessory Kit G2260-68705 113
Maintenance Kit G1313-68730 for G1329A 114
Maintenance Kit G1313-68719 for G1329B 115
Multi-Draw Kit G1313-6871 116
900 µl Injection Upgrade Kit G1363A for G1329A 117
External Tray G1313-60004 118

Agilent Technologies 105

8 Parts and Materials for Maintenance
Main Assemblies

Main Assemblies

&% &

'

+ *

Figure 23 Autosampler Main Assemblies

106
 Parts and Materials for Maintenance 8
Main Assemblies

Table 19 Autosampler Main Assemblies

Item Description Part Number

1 Transport assembly for G1329A-2260A G1329-60009

2 Sampling unit assembly for G1329A G1329-60008

Sampling unit assembly for G2260A G2260-60008
(The assy comes without injection valve and analytical head)

3 Analytical head assembly (100 µl) for G1329A and G1329B 01078-60003
Preparative head assembly (900 µl) for G1329A (P<200Bar) G1313-60007
Preparative head assembly (900 µl) for G2260A (P<400Bar) G2260-60007

4 Injection valve assembly for G1329A 0101-0921

Injection valve assembly for G1329B 0101-1422
Injection valve assembly for G2260A 0101-1267

5 Vial tray, thermostatted (see “Vial Trays” on page 111) G1329-60011

6 Gripper assembly G1313-60010

7 Illumination assembly G1367-60040

8 Autosampler Main Board (ASM) for G1329A and 2260A G1329-69530

Autosampler Main Board (ASM) for G1329B G1329-66540

Standoff - GPIB connector (part not shown) 0380-0643

Standoff - remote connector (part not shown) 1251-7788

9 Ribbon cable, sample transport G1313-81601

10 Ribbon cable, sampling unit G1313-81602

Sampler - TCC cap (380 mm 0.1 mm id) for G1329A 01090-87306

Sampler - Column cap (600 mm, 0.5 mm id) for G2260A G2260-87300

Power supply assembly (part not shown) 0950-2528

Screw M4, 8 mm lg - power supply (part not shown) 0515-0910

BCD board (not shown) G1351-68701

Cable, autosampler to ALS thermostat (part not shown) G1330-81600

107
8 Parts and Materials for Maintenance
Analytical-Head Assembly

Analytical-Head Assembly

Table 20 Analytical-Head Assembly (100 µl) for G1329A / G1329B

Item Description Part Number

Analytical head assembly, includes items 1 – 6 01078-60003

1 Plunger assembly 5063-6586

2 Screw M4, 40 mm lg, for mounting of assembly 0515-0850

3 Adapter 01078-23202

4 Support seal assembly 5001-3739

5 Metering seal (pack of 2) 5063-6589

6 Head body 01078-27710

7 Screw M5, 60 mm lg, for mounting of assembly (not shown 0515-2118

here)

108
 Parts and Materials for Maintenance 8
Analytical-Head Assembly

&

'

+
,cdih]dlc

Figure 24 Analytical-Head Assembly

109
8 Parts and Materials for Maintenance
Analytical-Head Assembly

Table 21 Preparative-Head Assembly (900 µl) for G1329A only

Item Description Part Number

1
Analytical head assembly 900 µl , includes items 1 – 6 G1313-60007

1 Plunger assembly, 900 µl 5062-8587

2 Screws 0515-0850

3 Adapter 01078-23202

4 Support seal assembly, 900 µl 5001-3764

5 Metering seal, 900 µl 0905-1294

6 Head body, 900 µl G1313-27700

7 Screw M5, 60 mm lg, for mounting of assembly (not shown 0515-2118

here)
1
This head is limited to 200 Bars

Table 22 Preparative-Head Assembly (900 µl) for G2260A

Item Description Part Number

Preparative head assembly 900 µl1, includes items 1 – 6 G2260-60007

1 Plunger assembly, 900 µl 5062-8587

2 Screws 0515-0850

3 Adapter 01078-23202

4 Support seal assembly, 900 µl 5001-3764

5 Metering seal, 900 µl 0905-1294

6 Head body, 900 µl G2260-27700

7 Screw M5, 60 mm lg, for mounting of assembly (not shown 0515-2118

here)
1
This head is limited to 400 Bars. It can only be assembled on a sampling unit with
the description “supports 900 µl at 400 Bar.

110
 Parts and Materials for Maintenance 8
Vial Trays

Vial Trays

Table 23 Thermostatted Autosampler Vial Trays and Tray Base

Item Description Part Number

1 Adapter, air channel G1329-43200

2 Tray for 100 × 2-ml vials, thermostattable G1329-60011

3 Spring G1313-09101

4 Tray base for G1329A / G1329B / G2260A (includes items 4, 5). G1329-60000

5 Spring stud 0570-1574

Halftray for 40 × 2-ml vials (not shown) G1313-44512

Halftray for 15 × 6-ml vials (not shown) G1313-44513

&

*
'

Figure 25 Thermostatted Autosampler Vial Trays and Tray Base

111
8 Parts and Materials for Maintenance
Standard Autosampler Accessory Kit G1329-68725

Standard Autosampler Accessory Kit G1329-68725

Table 24 G1329A/G1329 B - Standard Autosampler Accessory Kit Contents G1329-68725

Description Part Number

Flexible tubing assy (120 cm) 5063-6527

Filter promo kit no PN

CAN cable, 1 m long 5181-1519

Screw cap vials, clear 100/pk 5182-0714

Blue screw caps 100/pk 5182-0717

Label halftray no PN

Vial instruction sheet no PN

Wrenches 1/4 - 5/16 inch 8710-0510

Rheotool socket wrench 1/4 inch 8710-2391

Hex key 4 mm, 15 cm long, T-handle 8710-2392

Hex key 9/64 mm, 15 cm long, T- handle 8710-2394

Hex key 2.5 mm, 15 cm long, straight handle 8710-2412

Finger caps x3 (reorder gives pack of 15) 5063-6506

Front door cooled autosampler no PN

Air channel adapter G1329-43200

Cover insulation no PN

Capillary 0.17 mm, 900 mm G1329-87300

Capillary heat exchanger 01090-87306

Note for Agilent 1200 Infinity Autosampler door upgrade no PN

112
 Parts and Materials for Maintenance 8
Preparative Autosampler Accessory Kit G2260-68705

Preparative Autosampler Accessory Kit G2260-68705

Table 25 G2260A - Preparative Autosampler Accessory Kit Contents G2260-68705

Description Part Number

Flexible tubing assy (120 cm) 5063-6527

Filter promo kit no PN

CAN cable, 1 m long 5181-1519

Screw cap vials, clear 100/pk 5182-0714

Blue screw caps 100/pk 5182-0717

Label halftray no PN

Wrenches 1/4 - 5/16 inch 8710-0510

Rheotool socket wrench 1/4 inch 8710-2391

Hex key 4 mm, 15 cm long, T-handle 8710-2392

Hex key 9/64 mm, 15 cm long, T- handle 8710-2394

Hex key 2.5 mm, 15 cm long, straight handle 8710-2412

Finger caps x3 (reorder gives pack of 15) 5063-6506

Front door cooled autosampler no PN

Air channel adapter G1329-43200

Tray for 15 x 6 ml vials (x2) G1313-44513

Union, loop extension 5022-2133

Seat extension capillary (500 µl) G1313-87307

Seat extension capillary (1500 µl) G1313-87308

Sampler - Column capillary G2260-87300

Pump - Sampler capillary G2260-87301

113
8 Parts and Materials for Maintenance
Maintenance Kit G1313-68730 for G1329A

Maintenance Kit G1313-68730 for G1329A

Table 26 Maintenance Kit for G1329A

Item Description Part Number

1 Rotor seal (Vespel) 0100-1853

2 Needle assembly (100 µl) G1313-87201

3 Needle-seat assembly 0.17 mm, 2.3 µl G1313-87101

114
 Parts and Materials for Maintenance 8
Maintenance Kit G1313-68719 for G1329B

Maintenance Kit G1313-68719 for G1329B

Table 27 Maintenance Kit for G1329A

Item Description Part Number

1 Rotor seal (PEEK) 0101-1416

2 Needle assembly (100 µl) G1313-87201

3 Needle-seat assembly 0.17 mm G1313-87101

4 Metering seal (pack of 2) 5063-6589

5 Finger caps (pack of 15) 5063-6506

115
8 Parts and Materials for Maintenance
Multi-Draw Kit G1313-6871

Multi-Draw Kit G1313-6871

Table 28 Multi-Draw Kit for G1329A and G1329B

Item Description Part Number

1 Seat capillary, 500 µl, 0.5 mm id G1313-87307

2 Seat capillary, 1500 µl, 0.9 mm id G1313-87308

2 Seat capillary, 5000 µl 0101-0301

3 Union 5022-6515

116
 Parts and Materials for Maintenance 8
900 µl Injection Upgrade Kit G1363A for G1329A

900 µl Injection Upgrade Kit G1363A for G1329A

Table 29 900 µl Injection Upgrade Kit for G1329A only

Item Description Part Number

1 Analytical Head, 900 µl G1313-60007

2 Loop Extension, 900 µl G1313-87303

3 Union, loop extension 5022-2133

4 Needle, 900 µl G1313-87202

117
8 Parts and Materials for Maintenance
External Tray G1313-60004

External Tray G1313-60004

Table 30 External Tray

Item Description Part Number

1 External tray G1313-60004

2 Disposal tube G1313-27302

118
9
Identifying Cables
Cable Overview 120
Analog Cables 122
Remote Cables 125
BCD Cables 130
External Contact Cable 132
CAN/LAN Cables 133
Auxiliary Cable 134
RS-232 Cables 135

Agilent Technologies 119

9 Identifying Cables
Cable Overview

Cable Overview

Never use cables other than the ones supplied by Agilent Technologies to ensure proper
NOTE functionality and compliance with safety or EMC regulations.

Type Description Part Number

Analog cables 3390/2/3 integrators 01040-60101

3394/6 integrators 35900-60750

Agilent 35900A A/D converter 35900-60750

General purpose (spade lugs) 01046-60105

Remote cables 3390 integrator 01046-60203

3392/3 integrators 01046-60206

3394 integrator 01046-60210

3396A (Series I) integrator 03394-60600

3396 Series II / 3395A integrator, see details in section

“Remote Cables” on page 125

3396 Series III / 3395B integrator 03396-61010

HP 1050 modules / HP 1046A FLD 5061-3378

HP 1046A FLD 5061-3378

Agilent 35900A A/D converter 5061-3378

HP 1040 diode-array detector 01046-60202

HP 1090 liquid chromatographs 01046-60202

Signal distribution module 01046-60202

BCD cables 3396 integrator 03396-60560

General purpose (spade Lugs) G1351-81600

Auxiliary Agilent 1100 Series vacuum degasser G1322-61600

120
 Identifying Cables 9
Cable Overview

Type Description Part Number

CAN cables Agilent 1100/1200 module to module,0.5m lg 5181-1516
Agilent 1100/1200 module to module, 1m lg 5181-1519

External Agilent 1100/1200 Infinity interface board to general G1103-61611

contacts purpose

GPIB cable Agilent 1100/1200 module to ChemStation, 1 m 10833A

Agilent 1100/1200 module to ChemStation, 2 m 10833B

RS-232 cable Agilent 1100/1200 module to a computer 34398A

This kit contains a 9-pin female to 9-pin female Null
Modem (printer) cable and one adapter.

LAN cable Twisted pair cross over LAN cable, (shielded 3m long) (for 5023-0203
point to point connection)

Twisted pair cross over LAN cable, (shielded 7m long) (for 5023-0202
point to point connection)

121
9 Identifying Cables
Analog Cables

Analog Cables

One end of these cables provides a BNC connector to be connected to

Agilent 1100/1200 Infinity modules. The other end depends on the instrument
to which connection is being made.

Agilent Modules to 3390/2/3 Integrators

Connector 01040-60101 Pin 3390/2/3 Pin Agilent Signal Name

module

1 Shield Ground

2 Not connected

3 Center Signal +

4 Connected to pin 6

5 Shield Analog -

6 Connected to pin 4

7 Key

8 Not connected

122
 Identifying Cables 9
Analog Cables

Agilent Modules to 3394/6 Integrators

Connector35900-60750 Pin 3394/6 Pin Agilent Signal Name

module

1 Not connected

2 Shield Analog -

3 Center Analog +

Agilent Modules to BNC Connector

Connector8120-1840 Pin BNC Pin Agilent Signal Name

module

Shield Shield Analog -

Center Center Analog +

123
9 Identifying Cables
Analog Cables

Agilent Modules to General Purpose

Connector01046-60105 Pin 3394/6 Pin Agilent Signal Name

module

1 Not connected

2 Black Analog -

3 Red Analog +

124
 Identifying Cables 9
Remote Cables

Remote Cables

One end of these cables provides a Agilent Technologies APG (Analytical

Products Group) remote connector to be connected to Agilent 1100/1200
Infinity modules. The other end depends on the instrument to be connected to.

Agilent Modules to 3390 Integrators

Connector 01046-60203 Pin 3390 Pin Agilent Signal Name Active

module (TTL)

2 1 - White Digital ground

NC 2 - Brown Prepare run Low

7 3 - Gray Start Low

NC 4 - Blue Shut down Low

NC 5 - Pink Not connected

NC 6 - Yellow Power on High

NC 7 - Red Ready High

NC 8 - Green Stop Low

NC 9 - Black Start request Low

125
9 Identifying Cables
Remote Cables

Agilent Modules to 3392/3 Integrators

Connector01046-60206 Pin 3392/3 Pin Agilent Signal Name Active

module (TTL)

3 1 - White Digital ground

NC 2 - Brown Prepare run Low

11 3 - Gray Start Low

NC 4 - Blue Shut down Low

NC 5 - Pink Not connected

NC 6 - Yellow Power on High

9 7 - Red Ready High

1 8 - Green Stop Low

NC 9 - Black Start request Low

Agilent Modules to 3394 Integrators

Connector01046-60210 Pin 3394 Pin Agilent Signal Name Active

module (TTL)

9 1 - White Digital ground

NC 2 - Brown Prepare run Low

3 3 - Gray Start Low

NC 4 - Blue Shut down Low

NC 5 - Pink Not connected

NC 6 - Yellow Power on High

5,14 7 - Red Ready High

6 8 - Green Stop Low

1 9 - Black Start request Low

13, 15 Not connected

126
 Identifying Cables 9
Remote Cables

START and STOP are connected via diodes to pin 3 of the 3394 connector.
NOTE

Agilent Modules to 3396A Integrators

Connector03394-60600 Pin 3394 Pin Agilent Signal Name Active

module (TTL)

9 1 - White Digital ground

NC 2 - Brown Prepare run Low

3 3 - Gray Start Low

NC 4 - Blue Shut down Low

NC 5 - Pink Not connected

NC 6 - Yellow Power on High

5,14 7 - Red Ready High

1 8 - Green Stop Low

NC 9 - Black Start request Low

13, 15 Not connected

Agilent Modules to 3396 Series II / 3395A Integrators

Use the cable part number: 03394-60600 and cut pin #5 on the integrator side.
Otherwise the integrator prints START; not ready.

127
9 Identifying Cables
Remote Cables

Agilent Modules to 3396 Series III / 3395B Integrators

Connector03396-61010 Pin 33XX Pin Agilent Signal Name Active

module (TTL)

9 1 - White Digital ground

NC 2 - Brown Prepare run Low

3 3 - Gray Start Low

NC 4 - Blue Shut down Low

NC 5 - Pink Not connected

NC 6 - Yellow Power on High

14 7 - Red Ready High

4 8 - Green Stop Low

NC 9 - Black Start request Low

13, 15 Not connected

Agilent Modules to HP 1050, HP 1046A or Agilent 35900 A/D Converters

Connector5061-3378 Pin HP Pin Agilent Signal Name Active

1050/.... module (TTL)

1 - White 1 - White Digital ground

2 - Brown 2 - Brown Prepare run Low

3 - Gray 3 - Gray Start Low

4 - Blue 4 - Blue Shut down Low

5 - Pink 5 - Pink Not connected

6 - Yellow 6 - Yellow Power on High

7 - Red 7 - Red Ready High

8 - Green 8 - Green Stop Low

9 - Black 9 - Black Start request Low

128
 Identifying Cables 9
Remote Cables

Agilent Modules to HP 1090 LC or Signal Distribution Module

Connector01046-60202 Pin HP 1090 Pin Agilent Signal Name Active

module (TTL)

1 1 - White Digital ground

NC 2 - Brown Prepare run Low

4 3 - Gray Start Low

7 4 - Blue Shut down Low

8 5 - Pink Not connected

NC 6 - Yellow Power on High

3 7 - Red Ready High

6 8 - Green Stop Low

NC 9 - Black Start request Low

Agilent Modules to General Purpose

Connector01046-60201 Pin Universal Pin Agilent Signal Name Active

module (TTL)

1 - White Digital ground

2 - Brown Prepare run Low

3 - Gray Start Low

4 - Blue Shut down Low

5 - Pink Not connected

6 - Yellow Power on High

7 - Red Ready High

8 - Green Stop Low

9 - Black Start request Low

129
9 Identifying Cables
BCD Cables

BCD Cables

One end of these cables provides a 15-pin BCD connector to be connected to

the Agilent 1200 Infinity modules. The other end depends on the instrument
to be connected to

Agilent Modules to General Purpose

Connector G1351-81600 Wire Color Pin Agilent Signal Name BCD Digit
module

Green 1 BCD 5 20

Violet 2 BCD 7 80

Blue 3 BCD 6 40

Yellow 4 BCD 4 10

Black 5 BCD 0 1

Orange 6 BCD 3 8

Red 7 BCD 2 4
Brown 8 BCD 1 2

Gray 9 Digital ground Gray

Gray/pink 10 BCD 11 800

Red/blue 11 BCD 10 400

White/green 12 BCD 9 200

Brown/green 13 BCD 8 100

not connected 14

not connected 15 +5V Low

130
 Identifying Cables 9
BCD Cables

Agilent Modules to 3396 Integrators

Connector03396-60560 Pin 3392/3 Pin Agilent Signal Name BCD Digit

module

1 1 BCD 5 20

2 2 BCD 7 80

3 3 BCD 6 40

4 4 BCD 4 10

5 5 BCD0 1

6 6 BCD 3 8

7 7 BCD 2 4

8 8 BCD 1 2

9 9 Digital ground

NC 15 +5V Low

131
9 Identifying Cables
External Contact Cable

External Contact Cable

5 1
10 6
15 11

One end of this cable provides a 15-pin plug to be connected to Agilent 1200
Infinity module’s interface board. The other end is for general purpose.

Agilent Modules Interface Board to general purposes

Connector G1103-61611 Color Pin Agilent Signal Name

module

White 1 EXT 1

Brown 2 EXT 1

Green 3 EXT 2

Yellow 4 EXT 2

Grey 5 EXT 3

Pink 6 EXT 3

Blue 7 EXT 4
Red 8 EXT 4

Black 9 Not connected

Violet 10 Not connected

Grey/pink 11 Not connected

Red/blue 12 Not connected

White/green 13 Not connected

Brown/green 14 Not connected

White/yellow 15 Not connected

132
 Identifying Cables 9
CAN/LAN Cables

CAN/LAN Cables

Both ends of this cable provide a modular plug to be connected to Agilent 1200
Infinity module’s CAN or LAN connectors.

CAN Cables

Agilent 1200 module to module, 0.5 m 5181-1516

Agilent 1200 module to module, 1 m 5181-1519

Agilent 1200 module to control module G1323-81600

LAN Cables

Description Part number

Cross-over network cable (shielded, 3 m long), (for point to 5023-0203

point connection)

Twisted pair network cable (shielded, 7 m long) (for hub 5023-0202

connections)

133
9 Identifying Cables
Auxiliary Cable

Auxiliary Cable

One end of this cable provides a modular plug to be connected to the

Agilent 1100 Series vacuum degasser. The other end is for general purpose.

Agilent 1200 Infinity Series Degasser to general purposes

Connector G1322-81600 Color Pin Agilent Signal Name

module

White 1 Ground

Brown 2 Pressure signal

Green 3

Yellow 4

Grey 5 DC + 5 V IN

Pink 6 Vent

134
 Identifying Cables 9
RS-232 Cables

RS-232 Cables

Description Part number

RS-232 cable, instrument to PC, 9-to-9 pin (female) This cable 24542U
has special pin-out, and is not compatible with connecting G1530-60600
printers and plotters.

RS-232 cable kit, 9-to-9 pin (female) and one adapter 9-pin 34398A
(male) 25-pin female. Suited for instrument to PC.

Cable Printer Serial & Parallel, is a SUB-D 9 pin female vs. 5181-1529
Centronics connector on the other end (NOT FOR FW
UPDATE).

This kit contains a 9-pin female to 9-pin female Null Modem 34398A
(printer) cable and one adapter. Use the cable and adapter to
connect Agilent Technologies instruments with 9-pin male
RS-232 connectors to most PCs or printers.

135
9 Identifying Cables
RS-232 Cables

136
10
Appendix
General Safety Information 138
Lithium Batteries Information 142
Radio Interference 143
Sound Emission 144
Agilent Technologies on Internet 145

Agilent Technologies 137

10 Appendix
General Safety Information

General Safety Information

General Safety Information

The following general safety precautions must be observed during all phases of
operation, service, and repair of this instrument. Failure to comply with these
precautions or with specific warnings elsewhere in this manual violates safety
standards of design, manufacture, and intended use of the instrument. Agilent
Technologies assumes no liability for the customer’s failure to comply with
these requirements.

Ensure the proper usage of the equipment.

WA R N I N G
The protection provided by the equipment may be impaired.
➔ The operator of this instrument is advised to use the equipment in a manner as
specified in this manual.

General
This is a Safety Class I instrument (provided with terminal for protective
earthing) and has been manufactured and tested according to international
safety standards.

138
 Appendix 10
General Safety Information

Operation
Before applying power, comply with the installation section. Additionally the
following must be observed.
Do not remove instrument covers when operating. Before the instrument is
switched on, all protective earth terminals, extension cords,
auto-transformers, and devices connected to it must be connected to a
protective earth via a ground socket. Any interruption of the protective earth
grounding will cause a potential shock hazard that could result in serious
personal injury. Whenever it is likely that the protection has been impaired,
the instrument must be made inoperative and be secured against any intended
operation.
Make sure that only fuses with the required rated current and of the specified
type (normal blow, time delay, and so on) are used for replacement. The use of
repaired fuses and the short-circuiting of fuse holders must be avoided.
Some adjustments described in the manual, are made with power supplied to
the instrument, and protective covers removed. Energy available at many
points may, if contacted, result in personal injury.
Any adjustment, maintenance, and repair of the opened instrument under
voltage should be avoided whenever possible. When inevitable, this has to be
carried out by a skilled person who is aware of the hazard involved. Do not
attempt internal service or adjustment unless another person, capable of
rendering first aid and resuscitation, is present. Do not replace components
with power cable connected.
Do not operate the instrument in the presence of flammable gases or fumes.
Operation of any electrical instrument in such an environment constitutes a
definite safety hazard.
Do not install substitute parts or make any unauthorized modification to the
instrument.
Capacitors inside the instrument may still be charged, even though the
instrument has been disconnected from its source of supply. Dangerous
voltages, capable of causing serious personal injury, are present in this
instrument. Use extreme caution when handling, testing and adjusting.

139
10 Appendix
General Safety Information

When working with solvents please observe appropriate safety procedures

(e.g. goggles, safety gloves and protective clothing) as described in the material
handling and safety data sheet by the solvent vendor, especially when toxic or
hazardous solvents are used.

140
 Appendix 10
General Safety Information

Safety Symbols
Table 31 Safety Symbols

Symbol Description

The apparatus is marked with this symbol when the user should refer to the instruction manual
in order to protect risk of harm to the operator and to protect the apparatus against damage.

Indicates dangerous voltages.

Indicates a protected ground terminal.

Indicates eye damage may result from directly viewing the light produced by the deuterium lamp
used in this product.

The apparatus is marked with this symbol when hot surfaces are available and the user should
not touch it when heated up.

A WARNING
WA R N I N G
alerts you to situations that could cause physical injury or death.
➔ Do not proceed beyond a warning until you have fully understood and met the
indicated conditions.

A CAUTION
CAUTION
alerts you to situations that could cause loss of data, or damage of equipment.
➔ Do not proceed beyond a caution until you have fully understood and met the
indicated conditions.

141
10 Appendix
Lithium Batteries Information

Lithium Batteries Information

Lithium batteries may not be disposed-off into the domestic waste. Transportation of
WA R N I N G
discharged Lithium batteries through carriers regulated by IATA/ICAO, ADR, RID,
IMDG is not allowed.
Danger of explosion if battery is incorrectly replaced.
➔ Discharged Lithium batteries shall be disposed off locally according to national
waste disposal regulations for batteries.
➔ Replace only with the same or equivalent type recommended by the equipment
manufacturer.

Lithiumbatteri - Eksplosionsfare ved fejlagtig håndtering.

WA R N I N G
Udskiftning må kun ske med batteri af samme fabrikat og type.
➔ Lever det brugte batteri tilbage til leverandøren.

Lithiumbatteri - Eksplosionsfare.
WA R N I N G
Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten.
➔ Brukt batteri returneres appararleverandoren.

Bij dit apparaat zijn batterijen geleverd. Wanneer deze leeg zijn, moet u ze niet weggooien
NOTE maar inleveren als KCA.

142
 Appendix 10
Radio Interference

Radio Interference

Cables supplied by Agilent Technoligies are screened to provide opitimized

protection against radio interference. All cables are in compliance with safety
or EMC regulations.

Test and Measurement

If test and measurement equipment is operated with unscreened cables, or
used for measurements on open set-ups, the user has to assure that under
operating conditions the radio interference limits are still met within the
premises.

143
10 Appendix
Sound Emission

Sound Emission

Manufacturer’s Declaration
This statement is provided to comply with the requirements of the German
Sound Emission Directive of 18 January 1991.
This product has a sound pressure emission (at the operator position) < 70 dB.
• Sound Pressure Lp < 70 dB (A)
• At Operator Position
• Normal Operation
• According to ISO 7779:1988/EN 27779/1991 (Type Test)

144
 Appendix 10
Agilent Technologies on Internet

Agilent Technologies on Internet

For the latest information on products and services visit our worldwide web
site on the Internet at:
http://www.agilent.com
Select Products/Chemical Analysis
It will provide also the latest firmware of the Agilent 1200 Infinity modules for
download.

145
Index

Index

A connecting GPIB 38, 40 ESD (electrostatic discharge) strap 78,

connecting LAN 38, 40 78
Agilent
external contact 132 external contact
on internet 145
external contacts 121 cable 121, 132
air circulation 26
GPIB 121
algae information 54 LAN 121, 133 F
ambient operating temperature 27 remote 120, 125
failure 73
ambient non-operating temperature 27 RS-232 121, 135
flow connections 48
analog cables
cable 120, 122 frequency range 27
overview 120
analytical head 14 fuses 24
CAN
autosampler accessory kit contents 112, cable 133
113 capillaries 48
G
auxiliary choice of vials and caps 68 GPIB
cable 120, 134 cleaning 79 cable 121
condensation 26 gripper fingers 16
B gripper
battery D alignment 73
safety information 142 external vials 73
damaged packaging 34, 34
BCD
delay volume 51, 58, 58 H
cable 120, 130
delay 51
bench space 26, 26 half trays 56
delivery checklist 34, 34
bypass 11 humidity 27
dimensions 27
bypassing the autosampler 58
draw speed 68, 68
I
DRAW 68
C information
cable E on sound emission 144
analog 120, 122 injection valve 8, 13
eject speed 68, 68
auxiliary 120, 134 injection volume precision 68
BCD 120, 130 EJECT 68
injection volumes less than 2 µl 68
CAN 133 electrical connections
injection volumes 68
connecting APG remote 38, 40 descriptions of 19
injection sequence 11
connecting the ChemStation 38, 40 electrostatic discharge (ESD) 104
injection valve 15
connecting the power 38, 40 environment 24, 26
installation
connecting CAN 38, 40 error messages 73

146
Index

power cords 25 N autosampler main assemblies 107

installing the autosampler preparative-head assembly 110
needle drive 13, 14
sample trays 56 performance specifications
non-operating altitude 27
installing the sample tray 50 autosampler 28, 29, 30
non-operating temperature 27
installing the thermostatted autosampler preparative autosampler 31
numbering of vials 56
power cable and interface cable 46 physical specifications 34
preparation 45 physical specifications 27
installing the thermostatted autosampler
O Power Consideration 24
interface cables 44 operating Altitude 27 power consumption 27
power cable 44 operating temperature 27 power cords 25
safety 41, 44 optimizing performance power requirements 24
tray cover and front cover 58 automated needle wash 68 preparative autosampler accessory kit
installing the tray cover front door 58 delay volume 68 contents 36
installing the autosampler delay-volume adjustment 68 preparative head 14
flow connections 48 inject-valve seal 68
interface cables 41 low-volume capillary kit 68
R
safety 41 maintenance 68
optimizing performance radio interference 143
installing the autosampler 41
bypassing the autosampler 58 remote
power cable 41
low-volume capillary kit 58 cable 120, 125
internet 145
minimizing delay volume 51, 58, 58 repair procedures 80
introduction to the autosampler 8
repairs
L P metering plunger 98
metering seal 98
LAN park transport assembly 34
needle assembly 86
cable 121, 133 park transport assembly 58
needle-seat assembly 89
line frequency 27 parts and materials rotor seal 94
line voltage 27 900 µl injection upgrade kit 117 simple repairs 85
accessory kit 112 stator face 91
lithium batteries 142
external tray 118 using the ESD strap 78, 78
low volume injections 68
main assemblies 106
low-volume capillary kit 58 RS-232
maintenance kit 114
cable 121
multi-draw kit 116
M preparative ALS acc. kit 113
RS-232C
cable 135
mainpass 11 standard autosampler accessory
maintenance functions 73, 84 kit 112
vial trays and tray base 111 S
metering device 13, 68
metering seal 900 µl 110, 110 parts and materials safety class I 138
metering seal 108 analytical-head assembly (optional safety information
900 microlitre) 108 lithium batteries 142
missing parts 34
analytical-head assembly 108 safety
multi-draw option 8

147
Index

general information 138, 138 vial numbering 56

standards 27 vial racks 8
symbols 141 vial tray 58
sample trays 56 vials 8
numbering of vial positions 57 viscous samples 68, 68
sampling unit 13 voltage range 27
sampling sequence 10 volume 51
seals
metering seal 108, 110, 110 W
shipping 34, 58
weight 26, 27
simple repairs 85
site requirements 24
X
sound emission 144
X-axis 16
specification
physical 27
specifications 28, 34
Z
stack configuration 38, 40 Z-axis 16
rear view 38, 40
standard autosampler accessory kit
contents 35
stator 15
status indicator 73
step functions 73
stepper motor 14

T
temperature 28
theta-axis 16
transport assembly 16
transport mechanism 8
transport 58

U
unpacking the autosampler 34, 34

V
valve capillaries 48
vial contents temperature 28

148
Index

149
www.agilent.com

In This Book

This manual contains user information about

the Agilent 1260 Infinity Preparative
Autosampler.
The manual describes the following:
• introduction to the autosampler,
• site requirements and specifications
• installing the autosampler,
• using the autosampler,
• optimizing performance,
• troubleshooting and diagnostics,
• maintenance,
• parts and materials,
• cable overview,
• safety and warranty.

© Agilent Technologies 2006, 2008-2010

Printed in Germany
06/2010

*G2260-90000*
*G2260-90000*
G2260-90000

Agilent Technologies