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Spectrophotometric Instruments Can Be Classified Into Different Types Based On Their Design and

Known priscriotion

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Abdirazak Ali
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9 views2 pages

Spectrophotometric Instruments Can Be Classified Into Different Types Based On Their Design and

Known priscriotion

Uploaded by

Abdirazak Ali
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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1. spectrophotometer is a colour measurement device used to capture and evaluate colour.

2. Beer's law, also known as the Beer-Lambert law, is a fundamental principle in


spectrophotometry that describes the relationship between the absorption of light by a
substance and the properties of that substance.
3. Spectrophotometric instruments: are analytical devices used to measure the absorption
or transmission of light by a sample as a function of wavelength.
Spectrophotometric instruments can be classified into different types based on their design and
application, such as:

- UV-Vis spectrophotometers: Measure the absorption or transmission of ultraviolet and visible


light by the sample.
- Infrared (IR) spectrophotometers: Measure the absorption or transmission of infrared light by
the sample.
- Atomic absorption (AA) spectrophotometers: Measure the absorption of light by atoms in a
sample to determine the concentration of specific elements.
- Fluorescence spectrophotometers: Measure the emission of light by a sample after it has been
excited by a specific wavelength of light.
4. components of a spectrophotometric include are:
1. Light source: This can be a tungsten lamp, deuterium lamp, or other suitable light source that
provides a continuous spectrum of light.
2. Monochromatic: This is a device that selects a specific wavelength or narrow range of
wavelengths from the light source. This can be done using prisms, diffraction gratings, or filters.
3. Sample holder: This is where the sample to be analysed is placed. The sample can be in
liquid, solid, or gas form.
4. Detector: This component measures the intensity of the light that passes through or is
absorbed by the sample. Common detectors include photodiodes, photomultiplier tubes, or
charge-coupled devices (CCDs).
5. Data processing and display: The instrument converts the detected light intensity into a
digital signal and displays the results, often in the form of a spectrum or a numerical value.
4. Quality assurance (QA) of a spectrophotometer: is essential to ensure accurate and
reliable measurements. Such as calibration with standard solutions, monitoring
instrument performance and conducting regular maintenance.
5. Atomic Absorption Spectrophotometer (AAS) is an analytical instrument used to
determine the concentration of elements in a sample by measuring the absorption of light.

FLUOROMETRY
1. Fluorimeter is an analytical method to detect and measure fluorescence in
compounds like proteins, cells, or nucleotides.
2. Fluorescence polarization: (FP) is a technique that is widely used to monitor
binding events in solution. It can be used to assess bimolecular interactions, including
protein-antibody binding and
3. Advantages and disadvantages of fluorimetry:
Advantages
 Simple in construction
 Easy to use.
 Economical
Disadvantages
 It is not possible to use reference solution & Sample solution at a time.
 Rapid scanning to obtain Excitation & emission Spectrum of the compound is not
possible.
4. Chemiluminescence: is the generation of electromagnetic radiation as light by the
release of energy from a chemical reaction.
5. Turbidimetry: is an analytical technique that measures the turbidity or cloudiness of
a solution. It is commonly used to determine the concentration of suspended particles
or colloids in a liquid.

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