Operating Manual

C 170 (E3)

CO2 Incubator
with FPI-sensor system
and display controller RP1

Model Model version Door hinges Art. No.

C 170 C170-230V-R right 9040-0112, 9140-0112
C 170 C170-230V-L left 9040-0115, 9140-0115
C 170-UL C170UL-120V-R right 9040-0113, 9140-0113
C 170-UL C170UL-120V-L left 9040-0116, 9140-0116

BINDER GmbH
 Address: Post office box 102, 78502 Tuttlingen, Germany  Phone: +49 7462 2005 0
 Fax: +49 7462 2005 100  Internet: http://www.binder-world.com
 E-mail: [email protected]  Service Hotline: +49 7462 2005 555
 Service Fax: +49 7462 2005 93 555  Service E-Mail: [email protected]
 Service Hotline USA: +1 866 885 9794 or +1 631 224 4340 x3
 Service Hotline Asia Pacific: +852 390 705 04 or +852 390 705 03
 Service Hotline Russia and CIS: +7 495 988 15 16

Issue 07/2017 Art. No. 7001-0297

Contents

1. SAFETY .................................................................................................................. 5
1.1 Legal considerations ........................................................................................................................... 5
1.2 Structure of the safety instructions ...................................................................................................... 5
1.2.1 Signal word panel ..................................................................................................................... 5
1.2.2 Safety alert symbol ................................................................................................................... 6
1.2.3 Pictograms ................................................................................................................................ 6
1.2.4 Word message panel structure ................................................................................................. 7
1.3 Localization / position of safety labels at the chamber ....................................................................... 7
1.4 Type plate ........................................................................................................................................... 8
1.5 General safety instructions on installing and operating the chamber ................................................. 9
1.6 Precautions when working with CO2 gas .......................................................................................... 11
1.7 Precautions when handling gas cylinders ......................................................................................... 11
1.8 Intended use ..................................................................................................................................... 12
2. CHAMBER DESCRIPTION .................................................................................. 13
2.1 The CO2 measuring principle ............................................................................................................ 13
2.2 Chamber overview ............................................................................................................................ 14
2.3 Connection panel at the rear of the chamber.................................................................................... 16
2.4 Chamber doors ................................................................................................................................. 16
3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
INSTALLATION.................................................................................................... 17
3.1 Unpacking, and checking equipment and completeness of delivery ................................................ 17
3.2 Guidelines for safe lifting and transportation..................................................................................... 18
3.3 Storage .............................................................................................................................................. 18
3.4 Location of installation and ambient conditions ................................................................................ 18
4. INSTALLATION AND CONNECTIONS ............................................................... 21
4.1 Shelf holder and shelves ................................................................................................................... 21
4.2 CO2 sensor ........................................................................................................................................ 22
4.2.1 Connecting the CO2 sensor .................................................................................................... 22
4.2.2 General notes ......................................................................................................................... 22
4.3 Water pan .......................................................................................................................................... 23
4.4 Gas connection ................................................................................................................................. 24
4.4.1 Connecting the CO2 gas cylinder ............................................................................................ 25
4.4.2 Connecting the gas hose to the chamber rear ....................................................................... 26
4.4.3 Gas cylinder connection kit (option)........................................................................................ 27
4.5 Electrical connection ......................................................................................................................... 28
5. START UP ............................................................................................................ 29
6. FUNCTION OVERVIEW OF THE RP1 CONTROLLER ....................................... 29
6.1 Preset factory parameters ................................................................................................................. 30
6.2 Performance after turning on the chamber ....................................................................................... 30
7. CONTROLLER RP1 OPERATING MODES ......................................................... 31
7.1 Selecting and setting the operating functions ................................................................................... 32
7.2 Operating mode HAND ..................................................................................................................... 32
7.3 Operating mode USER: Advanced settings ...................................................................................... 34
7.4 Operating mode LOCK: Locking/unlocking of the operating functions’ settings by operating mode
HAND ................................................................................................................................................ 38
7.5 Performance during and after power failures and shut down ........................................................... 39

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8. CONTROLLER RP1 SETTINGS .......................................................................... 39
8.1 Altitude of the installation site above sea level ................................................................................. 39
8.2 Entering the set points of temperature and CO2 ............................................................................... 41
9. TEMPERATURE SAFETY DEVICES ................................................................... 42
9.1 Over temperature protective device (class 1) ................................................................................... 42
9.2 Safety controller (temperature safety device class 3.1) .................................................................... 42
9.2.1 Setting the safety controller set point type .............................................................................. 43
9.2.2 Setting the safety controller set point...................................................................................... 44
10. ALARM FUNCTIONS ........................................................................................... 46
10.1 Alarm functions overview .................................................................................................................. 46
10.2 Resetting the alarm messages ......................................................................................................... 46
10.3 Zero-voltage relay alarm output ........................................................................................................ 47
10.4 Safety controller temperature alarm.................................................................................................. 48
10.5 Temperature tolerance range alarm (high and low temperature) ..................................................... 48
10.6 Door open ......................................................................................................................................... 49
10.7 CO2 tolerance range alarm (CO2 over/under concentration) ............................................................ 50
10.8 CO2 pressure too low ........................................................................................................................ 50
10.9 Temperature sensor failure ............................................................................................................... 51
10.10 Failure of CO2 sensor ........................................................................................................................ 52
11. ERROR MESSAGES ............................................................................................ 52
12. REFERENCE MEASUREMENTS ........................................................................ 53
12.1 CO2 reference measuring ................................................................................................................. 53
12.1.1 Measuring the CO2 concentration indirectly via the pH of the cell medium ............................ 53
12.1.2 Measuring CO2 directly via chemical indicator tubes ............................................................. 54
12.1.3 Measuring CO2 directly with an electronic infrared measuring device ................................... 55
12.2 Temperature reference measurement .............................................................................................. 55
13. OPTIONS .............................................................................................................. 55
13.1 Silicone access ports 30 mm / 1.18 in, closable with 2 silicone plugs (8012-0558 rear, 8012-0559
left, 8012-0560 right) (option) ............................................................................................................ 55
13.2 Base on castors (option) ................................................................................................................... 56
13.3 Stacking adapter for direct thermal decoupled stacking (option) ...................................................... 56
13.1 Analog outputs for temperature and CO2 (option) ............................................................................ 56
14. AVOIDING MICROBIAL CONTAMINATION........................................................ 57
14.1 Cells and media ................................................................................................................................ 57
14.2 Laboratory conditions / equipment around the incubator.................................................................. 57
14.3 Working and behavior in the lab ....................................................................................................... 57
14.4 Chamber design and equipment ....................................................................................................... 58
14.5 Handling the CO2 incubator .............................................................................................................. 59
15. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION .... 60
15.1 Cleaning ............................................................................................................................................ 60
15.2 Decontamination / chemical disinfection of the CO2 incubator ......................................................... 61
15.3 Disinfection of the CO2 sensor .......................................................................................................... 63
15.4 Hot-air sterilization at 180 °C / 356 °F............................................................................................... 64
15.4.1 Overview ................................................................................................................................. 64
15.4.2 Procedure for hot-air sterilization: ........................................................................................... 64
15.5 Aborting the hot-air sterilization ........................................................................................................ 67
15.5.1 Aborting the hot-air sterilization manually ............................................................................... 68
15.5.2 Aborting the hot-air sterilization by opening the outer door .................................................... 69
15.5.3 Aborting the hot-air sterilization using the main power switch ................................................ 69

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16. MAINTENANCE, AND SERVICE ......................................................................... 70
16.1 Maintenance intervals, service .......................................................................................................... 70
16.2 Checking the air jacket heating fan ................................................................................................... 70
16.3 Gas inlet fine filter ............................................................................................................................. 70
16.4 Sending the chamber back to BINDER GmbH ................................................................................. 71
17. DISPOSAL ........................................................................................................... 72
17.1 Disposal of the transport packing...................................................................................................... 72
17.1.1 Outer chamber packing .......................................................................................................... 72
17.1.2 Packing inside the chamber, equipment ................................................................................. 72
17.2 Decommissioning .............................................................................................................................. 73
17.3 Disposal of the chamber in the Federal Republic of Germany ......................................................... 73
17.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of
Germany............................................................................................................................................ 74
17.5 Disposal of the chamber in non-member states of the EU ............................................................... 75
18. TROUBLESHOOTING ......................................................................................... 76
19. TECHNICAL DESCRIPTION ................................................................................ 79
19.1 Factory calibration and adjustment ................................................................................................... 79
19.2 Over current protection ..................................................................................................................... 79
19.3 Definition of usable volume ............................................................................................................... 79
19.4 C 170 technical data ......................................................................................................................... 80
19.5 Important conversion data for non-SI units ....................................................................................... 81
19.6 Conversion table for gas inlet pressures, bar – psi ........................................................................... 81
19.7 Equipment and options (extract) ....................................................................................................... 82
19.8 Accessories and spare parts (extract) .............................................................................................. 83
19.9 Dimensions C 170 ............................................................................................................................. 84
20. EU DECLARATION OF CONFORMITY ............................................................... 85
21. PRODUCT REGISTRATION ................................................................................ 87
22. CONTAMINATION CLEARANCE CERTIFICATE ............................................... 88
22.1 For chambers located outside the USA and Canada ....................................................................... 88
22.2 For chambers in the USA and Canada ............................................................................................. 91

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Dear Customer,
For the correct operation of the CO2 incubator C 170, it is important that you read this operating manual
completely and carefully and observe all instructions as indicated. Failure to read, understand and follow
the instructions may result in personal injury. It can also lead to damage to the chamber and/or poor
equipment performance.

1. Safety
This operating manual is part of the components of delivery. Always keep it handy for reference. The
device should only be operated by laboratory personnel especially trained for this purpose and familiar
with all precautionary measures required for working in a laboratory. Observe the national regulations on
minimum age of laboratory personnel. To avoid injury and damage observe the safety instructions in the
operating manual.

WARNING
Failure to observe the safety instructions.
Serious injuries and chamber damage.
 Observe the safety instructions in this operating manual
 Carefully read the complete operating instructions for the chamber.

1.1 Legal considerations

This operating manual is for informational purposes only. It contains information for installing, start-up,
operation and maintenance of the product. Note: the contents and the product described are subject to
change without notice.
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free
use and safety during operation and maintenance. In no event shall BINDER be held liable for any dam-
ages, direct or incidental arising out of or related to the use of this manual.
This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or
contact us directly by phone at the number located on page one of this manual
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or exist-
ing agreement, description, or legal relationship, nor do they modify such a relationship. All obligations on
the part of BINDER derive from the respective purchase contract, which also contains the entire and ex-
clusively valid statement of warranty administration. The statements in this manual neither augment nor
restrict the contractual warranty provisions.

1.2 Structure of the safety instructions

In this operating manual, the following safety definitions and symbols indicate dangerous situations in
accordance with the standards ISO 3864-2 and ANSI Z535.6.

1.2.1 Signal word panel

Depending on the seriousness and probability of serious consequences, potential dangers are identified
with a signal word, the corresponding safety color, and if appropriate, the safety alert symbol.

DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious
(irreversible) injury.

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 WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious
(irreversible) injury

CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor
(reversible) injury

CAUTION
Indicates a potentially hazardous situation, which, if not avoided, may result in damage to the product
and/or its functions or to property in its proximity.

1.2.2 Safety alert symbol

Use of the safety alert symbol indicates a risk of injury.

Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.

1.2.3 Pictograms

Warning signs

Electrical hazard Hot surface Explosive Atmosphere Stability hazard

Lifting hazard Gas cylinders CO2 suffocation and Pollution Hazard

poisoning hazard

Harmful substances Biohazard Risk of corrosion and /

or chemical burns
Mandatory action signs

Mandatory regulation Read operating Disconnect the power Lift with several persons
instructions plug

Environment protection Wear protective gloves Wear safety goggles

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Prohibition signs

Do NOT touch Do NOT spray with Do NOT climb

water

Information to be observed in order to ensure optimum function of the product.

1.2.4 Word message panel structure

Type / cause of hazard.

Possible consequences.
∅ Instruction on how to avoid the hazard: prohibition
 Instruction on how to avoid the hazard: mandatory action

Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.

1.3 Localization / position of safety labels at the chamber

The following labels are located on the chamber:
Pictograms (Warning signs) Service label

Hot surface

Risk of injury (UL chambers only).

Observe the safety instructions in the
operating manual.

Figure 1: Position of labels Figure 2: Position of labels

on the CO2 incubator C 170 on the CO2 incubator C 170-UL

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 Keep safety labels complete and legible.

Replace safety labels that are no longer legible. Contact BINDER Service for these replacements.

1.4 Type plate

Figure 3: Position of type plate

Nominal temp. 190 °C 1,10 kW / 6,1 A

374 °F 230 V / 50 Hz
IP protection 20 230 V / 60 Hz
Safety device DIN 12880 1N~
Class 3.1
Art. No. 9040-0112
Project No.
Built 2017 CO2 Incubator
BINDER GmbH
Im Mittleren Ösch 5
C170 Serial No. 00000000000000
78532 Tuttlingen / Germany Made in Germany
www.binder-world.com E3

Figure 4: Type plate C 170 (standard chamber, door hinged right)

Indications of the type plate Information

(example)
BINDER Manufacturer: BINDER GmbH
C 170 Model designation
CO2 Incubator Device name: CO2 Incubator
Serial No. 000000000000 Serial No. of the chamber
Built 2017 Year of construction
190 °C
Nominal temperature Nominal temperature
374 °F
IP protection 20 Type of IP protection acc. to standard EN 60529
Temp. safety device DIN 12880 Temperature safety device acc. to standard DIN 12880:2007
Class 3.1 Class of temperature safety device
Art. No. 9040-0112 Art. No. of the chamber
Project No. --- Optional: Special application acc. to project no.

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Indications of the type plate Information
(example)
1,10 kW Nominal power
6,1 A Nominal current
230 V / 50 Hz Nominal voltage ± 10%
230 V / 60 Hz at the indicated power frequency
1N~ Current type

Symbol on the type plate Information

CE conformity marking

Electrical and electronic equipment manufactured / placed on

the market in the EU after 13 August 2005 and to be disposed of
in a separate collection according to Directive 2012/19/EU on
waste electrical and electronic equipment (WEEE).
The chamber is certified by Underwriters Laboratories Inc.® ac-
cording to the following standards:
CAN/CSA-C22.2 No. 61010-1, 2nd Edition, 2004-07
(UL chambers only)
UL 61010-1, 2nd Edition, 2005-07-22
IEC 61010-1:2001, 2nd Edition and IEC 61010-2-10

1.5 General safety instructions on installing and operating the chamber

With regard to operating the chamber and to the installation location, please observe the DGUV guide-
lines 213-850 on safe working in laboratories (formerly BGI/GUV-I 850-0, BGR/GUV-R 120 or ZH 1/119,
issued by the employers’ liability insurance association) (for Germany).
BINDER GmbH is only responsible for the safety features of the chamber provided skilled electricians or
qualified personnel authorized by BINDER perform all maintenance and repair, and if components relat-
ing to chamber safety are replaced in the event of failure with original spare parts.
To operate the chamber, use only original BINDER accessories or accessories from third-party suppliers
authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.

CAUTION
Danger of overheating.
Damage to the chamber.
∅ Do NOT install the chamber in unventilated recesses.
 Ensure sufficient ventilation for dispersal of the heat.

Do not operate the chamber in hazardous locations.

DANGER
Explosion hazard.
Danger of death.
∅ Do NOT operate the chamber in potentially explosive areas.
∅ KEEP explosive dust or air-solvent mixtures AWAY from the chamber.

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The chamber does not dispose of any measures of explosion protection.

DANGER
Explosion hazard.
Danger of death.
∅ Do NOT introduce any substance into the CO2 incubator which is combustible or explo-
sive at working temperature.
∅ NO explosive dust or air-solvent mixture in the inner chamber.

Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of
the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature
inside the chamber must lie below the flash point or below the sublimation point of the charging material.
Familiarize yourself with the physical and chemical properties of the charging material, as well as the
contained moisture constituent and its behavior with the addition of heat energy and humidity.
Familiarize yourself with any potential health risks caused by the charging material, the contained mois-
ture constituent or by reaction products that may arise during the temperature process. Take adequate
measures to exclude such risks prior to putting the CO2 incubator into operation.

DANGER
Electrical hazard.
Danger of death.
∅ The chamber must NOT become wet during operation or maintenance.

The chambers were produced in accordance with VDE regulations and were routinely tested in accord-
ance to VDE 0411-1 (IEC 61010-1).
During and shortly after operation, the temperature of the inner surfaces almost equals the set-point.

CAUTION
The glass door, the glass door handle and the inner chamber will become hot during
operation.
Danger of burning.
∅ Do NOT touch the glass door, the glass door handle, the inner surfaces or the charging
material during operation.

WARNING
Stability hazard.
Danger of injury.
Damage to the chamber and the charging material.
Housing cover breakaway.
∅ Do NOT climb on the lower housing cover.
∅ Do NOT load the lower housing cover with heavy objects while the chamber door is
open.

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1.6 Precautions when working with CO2 gas
Carbon dioxide (CO2) in high concentrations is hazardous to health. It is colorless and almost odorless
and therefore practically imperceptible. Vent out any CO2 gas that may escape via good room ventilation
or a suitable connection to an exhaust system. We recommend installing a CO2 warning system.

WARNING
High concentration of CO2 (> 4 Vol.-%).
Risk of death by suffocation.
Danger of poisoning.
∅ Do NOT set up chambers in non-ventilated recesses
 Ensure technical ventilation measures
 Observe the relevant regulations for handling CO2.

1.7 Precautions when handling gas cylinders

General information for safe handling of gas cylinders:

• Store and use gas cylinders only in well-ventilated locations.
• Open the gas cylinder valve slowly to avoid pressure surges
• Secure gas cylinders during storage and use against falling (chaining).
• Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them.
• Always close the valve even with apparently empty cylinders; screw on the cap when not in
use. Return gas cylinders with the valve closed.
• Do not open gas cylinders by force. Mark them when damaged
• Protect gas cylinders against fire, e.g. do not store together with flammable liquids
• Observe relevant regulations for dealing with gas cylinders.

Secure the gas cylinders against falling and other mechanical damage.

WARNING
Safety valve tearing off.
Sudden release of the stored pressure energy.
Risk of injury.
 Secure gas cylinders against falling (chaining).
 Transport gas cylinders with a cylinder cart.

The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.

WARNING
Opening the cylinder valve when the cylinder is not connected.
Sudden release of the stored pressure energy.
Risk of injury.
 Close the gas cylinder valve before connecting or removing the gas hose.

After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or
diluted soap solution).

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1.8 Intended use
CO2 incubators C 170 are suitable for the cultivation of mammal cells under typical conditions of approx.
37 °C / 98.6 °F. The incubator permits setting defined pH conditions by common NaHCO3 buffer systems
of commercial cell media by keeping an exact CO2 atmosphere inside. The chambers guarantee high
humidity inside to avoid osmolarity increasing caused by the evaporation of the cell media.

Observing the instructions in this operating manual and conducting regular maintenance
work (chap. 16) is part of the intended use.

Other applications are not approved.

The chambers are not classified as medical devices as defined by the Medical Device Directive
93/42/EEC.

Due to the special demands of the Medical Device Directive (MDD), these ovens are not
qualified for sterilization of medical devices as defined by the directive 93/42/EWG.

WARNING: If customer should use a BINDER chamber running in non-supervised continu-

ous operation, we strongly recommend in case of inclusion of irrecoverable specimen or
samples to split such specimen or samples and store them in at least two chambers, if this is
feasible.

Always use proper protective equipment (clothing, gloves, safety glasses, etc.).
Always follow good hygienic practices according to GLP/SOP protocols.
Each individual operating the chamber is responsible for his or her own safety

Any component of the charging material must NOT be able to release toxic gases.

DANGER
Explosion or implosion hazard.
Danger of poisoning.
Danger of death.
∅ Do NOT introduce any substance combustible or explosive at working temperature into
the chamber, in particular no energy sources such as batteries or lithium-ion batteries.
∅ NO explosive dust or air-solvent mixture in the inner chamber.
∅ Do NOT introduce any substance which could lead to release of toxic gases.

In case of foreseeable use of the device there is no risk for the user through the integration of the cham-
ber into systems or by special environmental or operating conditions in the sense of EN 61010-1:2010.
For this, the intended use of the chamber and all its connections must be observed.

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2. Chamber description
The CO2 incubators C 170 are equipped with a microprocessor controller for temperature and CO2 levels
and a digital display accurate to one-tenth of a degree resp. 0.1 vol.-%
The inner chamber, the pre-heating chamber and the inside of the doors are all made of stainless steel
V2A (German material no. 1.4301, US equivalent AISI 304). The inner surfaces are smooth and therefore
easy to clean. The inner chamber is deep-drawn from one piece, polished (suitable for pharmaceutical
applications) and has no welds or inaccessible corners. The hinges and the seal of the inner glass door
are glued from the outside to aid cleaning of the inner chamber. When operating the chamber at high
temperatures (sterilization), the impact of the oxygen in the air may cause discoloration of the metallic
surfaces (yellowish-brown or blue) by natural oxidation processes. These colorations are harmless and
will in no way impair the function or quality of the chamber.
The perforated shelves and the shelf holder are also made of stainless steel (German material no.
1.4016, US equivalent AISI 430). You can insert a maximum of 8 shelves. The shelf holder is demounta-
ble without any tool. In this way, you can clean it hygienically in every laboratory dishwasher before hot-
air sterilization.
The housing is RAL 7035 powder-coated. All corners and edges are also completely coated.
The heating system of the CO2 incubator permits hot-air auto-sterilization at 190 °C / 374°F. Thus, a tem-
perature of at least 180 °C / 356°F is maintained on all internal surfaces, resulting in sterilization of the
entire inner chamber.
Thanks to the standard safety device (class 3.1 according to DIN 12880:2007), the set temperature is
maintained in case of failure.
The gas enters the chamber via a fine filter (aseptic filter) with a high filtration efficiency that also filters
the smallest particles.
A highly precise, drift-free CO2 infrared measuring system in combination with the permanent mixture of
CO2 gas through a special proprietary gas mixing head developed by BINDER allows precise and con-
stant CO2 concentrations for long periods. This creates optimum growth conditions for cultures. The CO2
sensor can be removed from the inner chamber by hand and cleaned with suitable detergents if needed.
The CO2 incubator can be operated in a temperature range from 7 °C / 12.6 °F above ambient tempera-
ture up to +50 °C / 122°F and a CO2 range of 0 vol.-% up to 20 vol.-%.

2.1 The CO2 measuring principle

Fast reaction times, as well as the highest accuracy and selectivity, characterize the CO2 measuring pro-
cedure of the C 170 incubator. The accuracy of the CO2 measuring system is based on a single-beam
infrared measuring cell, which measures in differential mode using the permanently alternating transmis-
sion feature of its semi-conductor filter.
Due to this highly developed single-beam principle with Fabry-Perot interferometer (FPI), disturbance
variables and aging phenomena in the measuring system are almost completely eliminated, so that this
measuring system, in contrast to other measuring procedures, remains practically drift-free between cali-
brations and is absolutely selective for CO2.
The CO2 measuring cell contains a measuring section inside, in which the absorption of infrared light
depends on the number of CO2 molecules in the beam path. This number of CO2 molecules changes with
the ambient pressure in relation to a constant volume. The distances between the molecules are conse-
quently pressure-dependent. The collision frequency of the IR-beam with CO2 molecules increases there-
fore by increasing pressure. For this reason, the ambient pressure must be compensated in order to cor-
rect the display reading of the CO2 concentration in vol.-%. This is achieved by entering the altitude of the
site above the sea (chap. 8.1).

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2.2 Chamber overview

(1)

(2)

(3)

Figure 5: CO2 incubator C 170, closed

(1) Triangle instrument panel with RP1 controller for temperature and CO2 and
(2) Door handle
(3) Main power switch

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 (11) (10) (9) (8) (7) (1)

(4) (5) (6)

Figure 6: CO2 incubator C 170, outer door open

(4) Glass door handle

(5) Water pan
(6) Shelves
(7) Shelf holder
(8) Pt 100 temperature sensor
(9) CO2 sensor
(10) Gas mixing head for CO2
(11) Silicone measuring port in the glass door

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2.3 Connection panel at the rear of the chamber

(12) (13) (14) (15) (16)

Figure 7: Rear connection panel

(12) Miniature fuses

(13) Connection for IEC connector plug
(14) Ethernet interface for computer communication (option)
(15) Connection socket for zero-voltage relay alarm contact
(16) Quick acting closure socket for CO2 gas cylinder

2.4 Chamber doors

The outer chamber door is equipped with a heater on its inner side. The door must be closed while the
chamber is operating normally in order to ensure stable climatic conditions in the inner chamber.
An additional glass door enables viewing of the samples without disturbing the temperature in the interior
and contaminating the samples sealing the interior of the chamber.
When the outer door is open, the CO2 intake valve automatically closes.

Delay time for the temperature and CO2 tolerance range alarm :
After closing the outer door, the tolerance range alarm is turned off for a programmable delay
time. This prevents alarms being constantly triggered during the unstable operating phase
after opening the outer door.

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3. Completeness of delivery, transportation, storage, and installa-
tion
3.1 Unpacking, and checking equipment and completeness of delivery
After unpacking, please check the chamber and its optional accessories, if any, based on the delivery
receipt for completeness and for transportation damage. Inform the carrier immediately if transportation
damage has occurred.
The final tests of the manufacturer may have caused traces of the shelves on the inner surfaces. This has
no impact on the function and performance of the chamber.
Please remove any transportation protection devices and adhesives in/on the chamber and on the doors
and remove the operating manuals and accessory equipment.
For transport purpose, a silica gel bag for drying purpose was added. Do not eat! Do not open the silica
gel bag and dispose of it with normal waste.

Remove any protective lamination sheet on the inner metal surfaces prior to commissioning.

CAUTION
Sliding or tilting of the chamber.
Damage to the chamber.
Risk of injury by lifting heavy loads.
∅ Do NOT lift or transport the chamber using the door handle or the door.
 Lift the chamber from the pallet at the four lower corners with the aid of four people.

If you need to return the chamber, please use the original packing and observe the guidelines for safe
lifting and transportation (chap. 3.2).
For disposal of the transport packing, see chap. 17.1.
Note on second-hand chambers (Ex-Demo-Units):
Second-hand chambers are chambers that were used for a short time for tests or exhibitions. They are
thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work
flawlessly.
Second-hand chambers are marked with a sticker on the chamber door. Please remove the sticker before
commissioning the chamber.

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3.2 Guidelines for safe lifting and transportation
After operation, please observe the guidelines for temporarily decommissioning the chamber (chap. 17.2).
Empty the water pan before moving the incubator. In case of any spilling of the contents, shut down the
incubator and dry it out carefully and completely

CAUTION
Sliding or tilting the chamber.
Damage to the chamber.
Risk of injury by lifting heavy loads.
 Transport the chamber in its original packaging only.
 Secure the CO2 incubator with transport straps for transport.
∅ Do NOT lift or transport the chamber using the door handle or the door.
 Lift the chamber at the four lower corners with the aid of 4 people and place it on a
rolling pallet.
 Move the chamber to the desired location and lift it from the rolling pallet with the aid of
four people.

• Permissible ambient temperature range for transport: -10 °C / 14°F to +60 °C / 140°F.
You can order transport packing and rolling pallets for transportation purposes from BINDER Service.

3.3 Storage
Intermediate storage of the chamber is possible in a closed and dry room. Observe the guidelines for
temporary decommissioning (chap. 17.2).
• Permissible ambient temperature range for storage: -10 °C / 14°F to +60 °C / 140°F.
• Permissible ambient humidity: max. 70% r.H., non-condensing
When after storage in a cold location you transfer the chamber to its warmer installation site, condensa-
tion may form in the inner chamber, on the housing or in the sensor compartment of the CO2 measure-
ment. Before start-up, wait at least one hour until the CO2 incubator has attained ambient temperature
and is completely dry.

3.4 Location of installation and ambient conditions

Notes on the location of installation
Set up the CO2 incubator on a flat, even surface, free from vibration and in a well-ventilated, dry location.
The chambers are designed for setting up inside a building (indoor use).
Freestanding chambers are suitable for installation on tables or on the optionally available stand. Note:
The site of installation must be capable of supporting the chamber’s weight (see technical data, chap.
19.4).
Align the chamber using a spirit level to ensure even covering of the cell-cultures with the medium. For
this purpose, manually adjust the four incubator feet.
In order to avoid contamination, never place the chamber directly on the floor.

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 CAUTION
Danger of overheating.
Damage to the chamber.
∅ Do NOT set up chambers in non-ventilated recesses.
 Ensure sufficient ventilation for dispersal of the heat.

To completely separate the chamber from the power supply, you must disconnect the power plug. Install
the chamber in a way that the power plug is easily accessible and can be easily pulled in case of danger.
For the user there is no risk of temporary overvoltages in the sense of EN 61010-1:2010.
Do not install or operate the chamber in potentially explosive areas.

DANGER
Explosion hazard.
Danger of death.
∅ Do NOT operate the chamber in potentially explosive areas.
∅ KEEP explosive dust or air-solvent mixtures AWAY from the vicinity of the chamber.

Ambient conditions
• Permissible ambient temperature range for operation: +18 °C / 64.4 °F up to +30 °C / 86 °F. At elevat-
ed ambient temperature values, fluctuations in temperature can occur.
• Ideal ambient temperature: by at least 7 °C / 12.6 °F below the intended working temperature. E.g.,
working temperature 37 °C / 98.6 °F – resulting permitted ambient temperature 30 °C / 86°F and lower
In the event of working temperatures of less than 7 °C / 12.6 °F above the ambient temperature, the set
point can be exceeded.

The ambient temperature should not be substantially higher than the indicated ambient tem-
perature of +22 °C +/- 3 °C / 71.6 °F ± 5.4 °F to which the specified technical data relate. For
other ambient conditions, deviations from the indicated data are possible.

Avoid direct solar radiation on the chamber.

• Permissible ambient humidity: 70% r.H. max., non-condensing.

• Installation height: max. 2000 m / 6562 ft. above sea level. After the incubator has been turned on for
the first time, enter the altitude of the site above sea level into the RP1 controller (chap. 8.1).
• Wall distances: rear 100 mm / 3.94 in, sides 50 mm / 1.97 in.

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Notes on handling carbon dioxide (CO2)
Carbon dioxide (CO2) in high concentrations is hazardous to health. It is colorless and almost odorless
and therefore practically imperceptible. Vent out any CO2 gas that may escape via good room ventilation
or a suitable connection to an exhaust system. We recommend installing a CO2 warning system.

WARNING
High concentration of CO2 (> 4 Vol.-%).
Danger of death by suffocation.
Danger of poisoning.
∅ Do NOT set up chambers in non-ventilated recesses.
 Ensure technical ventilation measures.
 Observe the relevant regulations for handling CO2.

Observe the occupational exposure limit OEL for CO2 set by the national authorities (formerly maxi-
mum permitted workplace concentration). Check compliance when operating all chambers located in the
room.
• OEL for Germany: 5000 ml/m3 (ppm) = 0,5 Vol.-%
• CO2 lost with each door opening: about 16.4 g, i.e. 0.0084 cubic meters / 0.296 cubic feet (under nor-
mal pressure)
• CO2 lost during 12h at 5 vol.-% without door opening: approx. < 2 g, i.e. 0.001 cubic meters / 0.035
cubic feet (under normal pressure 1013 mbar / 14.7 psi)
An example of how to evaluate laboratory volume and air change rate:
Question: Is an air change rate of 1/h sufficient for a lab with a volume of 100 cubic meters / 3,531.5
cubic feet with 10 CO2 incubators, opened 4 times per hour?
Calculation: CO2 concentration = CO2 lost by door opening, multiplied by 10 chambers, multiplied by 4
door openings per hour, divided by lab volume
0.0084 cubic meters x 10 x 4 div. 100 cubic meters = 0.00336, i.e. 0.336% or 3360 ppm.
0.296 cubic feet x 10 x 4 div. 3,531.5 cubic feet = 0.00336, i.e. 0.336% or 3360 ppm.
Result: The maximum permissible value of 5000 ppm is not exceeded under these operation conditions.

Even when CO2 or systems operated with CO2 are handled carefully and appropriately, a residual risk
remains, which can lead to life-threatening situations under certain circumstances. Therefore we strongly
recommend continuous monitoring of CO2 concentration in the ambient air of the CO2 incubator. It must
be ensured permanently that the maximum permissible occupational exposure limit OEL for CO2 (0.5 vol -
% CO2 for Germany) is not exceeded.

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4. Installation and connections
4.1 Shelf holder and shelves
The shelf holder consists of two identical side parts. Insert them alongside the lateral walls of the inner
chamber. They are fixed by cams on the bottom of the inner chamber. For assembly and disassembly,
fold both side parts towards the middle.
Then you can insert the shelves into the shelf holder. Hold the shelf straight and then insert it so it will go
smoothly inside the chamber.

Figure 8: Installation of the shelves and shelf holder

If just one of the three supplied shelves shall be used in one of the three lower positions, we
recommend inserting a supplementary shelf as high as possible into the shelf holder for stabi-
lizing purpose.

To remove the shelf holder, remove the shelves first.

It is not possible to take out the shelf holder entirely together with the shelves.

Figure 9: View of the interior with inserted

shelves and the position of the cams
to fix the shelf holder

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Permitted shelf loads:
Maximum load on one single shelf: 10 kg / 22 lb
Maximum total load on all shelves: 30 kg / 66 lb

4.2 CO2 sensor

4.2.1 Connecting the CO2 sensor

Turn off the chamber. Open the door of the inner chamber and plug the CO2 sensor (4) into the perma-
nently installed holding tube located in the upper part of the rear of the inner chamber.

Figure 10: Plugged-in CO2 sensor (right) and gas mixing head for CO2

The sensor must click in correctly and sit tightly in the connection socket.

CAUTION
Connecting or removing the CO2 sensor during operation.
Damage to the CO2 sensor.
 Connect or remove the CO2 sensor only with the chamber turned off.

4.2.2 General notes

Connect or remove the CO2 sensor without rotating and only when the incubator is turned off. Remove
the CO2 sensor before removing or replacing its filter cap. The PTFE filter of the CO2 sensor prevents dirt
and humidity from intruding into the measuring cell. It is available as a spare part. Replace it whenever it
is damaged or soiled.

The accuracy of the indicated values of CO2 depends on the ambient air pressure (approx.
0.08 vol.-% per 10 mbar / 0.15 psi). In order to compensate this effect when measuring the
CO2 concentration, the altitude of the installation site above sea level can be entered into the
controller (chap. 8.1).

The CO2 sensor is temperature resistant up to a maximum temperature of 60 °C / 140 °F.

CAUTION
Excess temperature.
Damage to the CO2 sensor.
∅ Do NOT autoclave the CO2 Sensor.
∅ Do NOT expose the CO2 sensor to hot-air sterilization.

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The CO2 sensor head was especially adjusted for the specific chamber. To avoid confusion, an adhesive
label with a serial number is adhered to the sensor head. When exchanging the sensor, you must repeat
the CO2 adjustment.

CAUTION
Different CO2 sensor.
Invalid calibration.
∅ Do NOT change the CO2 sensor head.
 Note down the serial number of the CO2 sensor.

Avoid strong shocks when handling the CO2 sensor.

CAUTION
Shocks of the CO2 sensor.
Damage to the CO2 sensor.
∅ Avoid strong shocks of the CO2 sensor (by putting it down hard, or dropping).

4.3 Water pan

The water pan permits high humidity without condensation on the inner walls of the CO2 incubator.
Place the water pan in central position on the bottom of the inner chamber. Place the water pan with its
narrow side centrally between the front cams on the bottom of the inner chamber.

Figure 11: Position of the water pan centrally between the front cams (arrows)

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Make sure that the water pan has firm contact with the inner chamber bottom and rests tightly on it.
Fill the water pan with 1 liter of distilled, sterilized water.
We recommend cleaning (chap. 15.1) and refilling the pans 2 to 3 times a week. For evacuation, remove
the water pan.
We recommend using distilled, sterile water to achieve optimum growth results. Any corrosive damage
that may arise following use of water of different quality or by additives is excluded from the liability
agreement.
If required, you can add microbiologically inhibiting substances such as copper chips, copper sulphate or
ethylene diamine tetra-vinegar acid (EDTA) in a concentration of 1 to 5 mmol/l.
Empty the water pan before moving the incubator. In case of the contents spilling, immediately shut down
the incubator and dry it carefully and completely.

4.4 Gas connection

General information for safe handling of gas cylinders:

• Store and use gas cylinders only in well ventilated areas.
• Open the gas cylinder valve slowly to avoid pressure surges
• Secure gas cylinders during storage and use against falling (chaining).
• Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them
• Always close the valve even with apparently empty cylinders; screw on the cap when not in
use. Return gas cylinders with the valve closed
• Do not open gas cylinders by force. Mark them when damaged
• Protect gas cylinders against fire, e.g. do not store together with flammable liquids
• Observe relevant regulations for dealing with gas cylinders.

Secure the gas cylinders against falling and other mechanical damage.

WARNING
Safety valve tearing off.
Sudden release of the stored pressure energy.
Risk of injury.
 Secure gas cylinders against falling (chaining).
 Transport gas cylinders with a cylinder cart.

The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.

WARNING
Opening the cylinder valve when the cylinder is not connected.
Sudden release of the stored pressure energy.
Risk of injury.
 Close the gas cylinder valve before connecting or removing the gas hose.

After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or
diluted soap solution).

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4.4.1 Connecting the CO2 gas cylinder

Carbon dioxide (CO2) in high concentrations is hazardous to health. It is colorless and almost odorless
and therefore practically imperceptible. Vent out any CO2 gas that may escape via good room ventilation
or a suitable connection to an exhaust system. We recommend installing a CO2 warning system.

WARNING
High concentration of CO2 (> 4 Vol.-%).
Danger of death by suffocation.
Danger of poisoning.
∅ Do NOT set up chambers in non-ventilated recesses.
 Ensure technical ventilation measures.
 Observe the relevant regulations for handling CO2.

The CO2 gas necessary for operation must have a technical grade of 99.5 %.

The gas connections must be established by qualified personnel who are trained in handling
the respective gases and familiar with the required safety measures.

The following steps are required:

Ensuring the correct CO2 output pressure

A gas supply pressure above 2.5 bar / 36 psi will result in chamber damage.

Use a pressure reducer and make sure to avoid any excessive outlet pressure when connecting the
gas hose to the incubator.
The real outlet pressure of gas cylinders, sets of gas cylinders or central gas supplies am on the sec-
ond manometer must not exceed 2.5 bar / 36 psi.

CAUTION
Excessive outlet pressure > 2.5 bar / 36 psi.
Damage to the chamber.
∅ The outlet pressure must NOT exceed the indicated value of 2.5 bar / 36 psi.
 Before connecting, check the outlet pressure on the pressure reducer of the cylin-
der.
 Adjust the outlet pressure to 2.0 bar / 29 psi above the ambient pressure.

Observe the correct outlet pressure also when replacing the gas cylinders.
Establishing the connection to the incubator
Connect the supplied gas hose (internal diameter 6 mm / 0.24 inches) to the pressure reducer of the
gas cylinders or central gas supply and secure the connection with the supplied hose clamp.
Connect the pre-assembled hose nozzle of the gas hose to the quick acting closure socket (14) DN 6
on the chamber rear, as described in chap. 4.4.2.
Leak test
After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap
solution).

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 The recovery times of the gas concentrations inside the chamber after opening the door are
indicated in the technical data (chap. 19.4) and refer to a connection pressure of 2.0 bar / 29
psi. Decreasing supply pressure will result in longer recovery times.

Conversion table for gas inlet pressures, bar – psi, see chap. 19.6.

4.4.2 Connecting the gas hose to the chamber rear

The gas hose, which will be used to establish the connection to a gas cylinder, is already attached to the
hose nozzle and secured by a hose clamp. Plug the hose nozzle into the corresponding quick acting clo-
sure socket (a) located at the rear of the chamber. This quick acting closure socket is closed by a rubber
cover (b).

Only use the supplied hose nozzle to connect to the quick acting closure socket.
Otherwise, the quick acting closure socket may leak, and/or it may become impossible to con-
nect the original hose nozzle. In this case, please contact BINDER Service.

Remove the rubber cover (b) by pulling it off.

Chamber rear

(a)

(b)

(c)

Figure 12: Connection of the hose lead to the gas cylinder

Now fit the hose nozzle (c) in the quick acting closure socket. To remove the connection, pull the hose
nozzle off the quick acting closure socket.

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4.4.3 Gas cylinder connection kit (option)

The gas cylinder connection kit for CO2 (Art. no. 8012-0014) includes the
following parts for connecting a gas cylinder to the CO2 incubator:
• Pressure reducer with manometers for cylinder pressure (high pressure
gauge) and outlet pressure (low pressure gauge)
• 5 m pressure hose with pre-assembled hose nozzle for quick acting clo-
sure socket
• 1 hose clamp to connect the gas hose to the pressure reducer
The pressure reducer is also available as an individual accessory.

Figure 13: Gas cylinder connection kit

Instructions 7001-0249 included with the connection kit describe connecting and setting
the gas cylinder connection kit.

CAUTION
Excessive outlet pressure > 2.5 bar / 36 psi.
Damage to the chamber.
∅ The outlet pressure must NOT exceed the indicated value of 2.5 bar / 36 psi.
 Before connecting, check the outlet pressure on the pressure reducer of the cylinder.
 Adjust the outlet pressure to 2.0 bar / 29 psi above the ambient pressure.

The gas connections must be established by qualified personnel who are trained in handling
the respective gases and familiar with the required safety measures.

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4.5 Electrical connection
The chambers are supplied ready for connection. They come with an IEC connector plug.

Nominal voltage ± 10% at the Current Chamber

Model Power plug
indicated power frequency type fuse
230 V at 50 Hz
C 170 Shock-proof plug 1N~ 10 A
230 V at 60 Hz
C 170-UL NEMA 5-20P 120 V at 60 Hz 1N~ 16 A

• The domestic socket must also provide a protective conductor. Make sure that the connection of the
protective conductor of the domestic installations to the chamber’s protective conductor meets the lat-
est technology. The protective conductors of the socket and plug must be compatible!
• Prior to connection and start-up, check the power supply voltage. Compare the values to the specified
data located on the chamber’s type plate (centrally located at the bottom of the left-hand side of the
chamber, see chap. 1.4)
• When connecting, please observe the regulations specified by the local electricity supply company
and as well as the VDE directives (for Germany). We recommend the use of a residual current circuit
breaker.
• Pollution degree (acc. to IEC 61010-1): 2
• Over-voltage category (acc. to IEC 61010-1): II

CAUTION
Danger of incorrect power supply voltage.
Damage to the equipment.
 Check the power supply voltage before connection and start-up.
 Compare the power supply voltage with the data indicated on the type plate.

See also electrical data (chap. 19.4).

To completely separate the chamber from the power supply, you must disconnect the power
plug. Install the chamber in a way that the power plug is easily accessible and can be easily
pulled in case of danger.

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5. Start up
After connecting the supply lines, turn on the chamber by the main power switch (2).
After turning on the incubator for the first time, enter the altitude of the site above sea level into the con-
troller RP1 (chap. 8.1).
Warming chambers may release odors in the first few days after commissioning. This is not a quality de-
fect. To reduce odors quickly we recommend heating up the chamber to its nominal temperature for one
day and in a well-ventilated location.

6. Function overview of the RP1 controller

The RP1 controller controls the following values inside the CO2 incubator:
• Temperature in °C (range 7 °C / 12.6 °F above ambient temperature up to 50 °C / 122 °F)
• Carbon dioxide concentration in vol.-% (range 0 vol.-% up to 20 vol.-%)

Figure 14: Overview controller RP1

Controller alarm and status LEDs

Symbol LED Meaning
red General alarm
yellow Heating active
red Temperature alarm
yellow CO2 valve open
red CO2 alarm

Controller buttons
Button Function

Turn off the alarm sound

Reduce value

Increase value

Confirm entry and call up next operating function

Start / stop the sterilization cycle

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 Figure 15: Normal Display of controller

Upper display: current inner chamber temperature

LED “ °C” lit: inner chamber temperature displayed in °C
Lower display: current CO2 concentration
LED “%CO2” lit: CO2 concentration displayed in vol.-% CO2

6.1 Preset factory parameters

The chamber is supplied with the following basic preset parameters:
Temperature set point 37 °C / 98.6°F
CO2 set point 5 vol.-%
Set point type “Offset”
Safety controller class 3.1
2.0 °C
Set point sterilization temperature 190 °C / 374 °F
Installation height above sea level 0.65 km / 2132.54 ft
The maximum permitted deviation from the temperature set point for
1.0 K
tolerance range alarm
Delay time tolerance range alarm temperature after opening the door 15 min
The maximum permitted deviation from the CO2 set point for tolerance
1.0 vol.-%
range alarm.
Delay time tolerance range alarm CO2 after opening the door 15 min
Password to lock / unlock the HAND operating functions 0
Password to access operating mode USER 1

6.2 Performance after turning on the chamber

During the equilibration phase of 2 hours after turning on the chamber, undefined temperature and CO2
conditions occur within the chamber. During this phase, do not place any sample materials in the cham-
ber.

CAUTION
Danger of samples being destroyed.
 Charge the chamber only after equilibration of temperature and CO2.

Check that the interior of the chamber is empty, including the trays and the water pan. If you do not know
what the chamber was last used for, for hygiene purposes you should clean and disinfect or sterilize the
interior (chap. 15).

WARNING: If customer should use a BINDER chamber running in non-supervised continu-

ous operation, we strongly recommend in case of inclusion of irrecoverable specimen or
samples to split such specimen or samples and store them in at least two chambers, if this is
feasible.

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Open the CO2 supply's pressure reducer valve and set a CO2 primary pressure of 2.0 bar / 29 psi.
Set the main power switch (2) to position I. The pilot lamp shows the chamber is ready for operation.
There is a subsequent brief startup phase in which the display items at the edges of the upper controller
display light up successively.
After a few seconds, the upper display shows the current interior temperature of the chamber and the
lower display shows the current CO2 interior concentration. The lit LEDs at the right of the display show
that the temperature is displayed in °C and the CO2 concentration is displayed in vol.-% CO2.

Figure 16: Normal display of controller after turning on the

incubator, showing the current temperature and CO2 val-
ues (example)

Set the controller to the desired temperature and CO2 set points that are to be used to operate the cham-
ber.
The yellow LED indicates that the heating is active, and the yellow LED indicates that the CO2
valve is open.
Only insert samples into the chamber when it has reached its stable operating state:
Temperature: Equilibration time is approx. 2 hours.
CO2: After approx. 5 minutes, the CO2 concentration equilibrates automatically to the preset value.

If there is no accordance between the actual and set value shown in the display, proper opera-
tion of the chamber is not guaranteed.

Instructions when observing leakage of condensate from the CO2 sensor system and CO2 fluctua-
tion:
In the case of CO2 fluctuations and concomitant condensate leakage from the injection and suction noz-
zle of the CO2 sensor compartment after start-up or when taking the chamber back into service, the
chamber should be left to dry open for at least an hour running at 37 °C with the water pan empty. This
will result in flushing the sensor compartment and tubing from humid air. Removing the condensate from
the CO2 sensor system will ensure a turbulence-free CO2 measurement.

7. Controller RP1 operating modes

The controller provides three operating modes HAND (chap. 7.2), USER (chap. 7.3), and LOCK (chap.
7.4). In operating modes HAND and USER, you can call up several operating functions and set / modify
their values.
Operating mode USER is password protected. On delivery, the password is set to “1” (factory setting).
You can change the password in operating function “PA.U” (chap. 7.3).
You can also implement password protection for operating mode HAND (chap. 7.4).

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7.1 Selecting and setting the operating functions
The controller's lower display always shows the operating function (e.g. “SP” = temperature set point) and
the upper display shows the associated value (e.g. 37.0).
When the value in the upper display shows the temperature in °C or a CO2 concentration in % CO2, the
corresponding yellow LED to the right of the display will light up.
Upper display: value of the operating function to be set
Lower display: type of operating function to be set
LED “ °C” lit: operating function value displayed in °C
LED “%CO2” lit: operating function value displayed in vol.-%
CO2
LED “ °C” and LED “%CO2” off: no or other unit of operating
function value

Figure 17: Controller display when setting operating functions,

here: setting of temperature set point

In operating modes HAND and USER, access the next operating function by pressing “MODE”.
Pressing “MODE” will confirm the changed value of the current operating function, i.e., the chamber will
from now on regulate at the new value.

If you do not want to accept the modification of a value, wait 30 seconds without pressing any
button. The controller reverts to Normal Display automatically without accepting the modifica-
tion.

Use “▲” and “▼” to change the value of the selected operating function or to select between defined
values.

To change numerical values by tenths, press “▲” and “▼” for a very short time. To change
them by whole numbers, press “▲” and “▼” for longer.

In some operating functions, you can read but not change the value (for service and maintenance pur-
poses).
To go back to the Normal display, press “MODE” repeatedly.
If a button for 30 seconds is not pressed after access to an operating function or changing a value, the
controller toggles back to Normal display automatically. If the “MODE” button is not pressed after chang-
ing a value in the displayed operating function, the value reverts to its previous value.

7.2 Operating mode HAND

• In Normal display, press button “MODE” to toggle to operating mode HAND.

• Use “MODE” to successively call up the operating functions.
• Use “▲” or “▼” to set the values of the operating functions.

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Operating functions that can be set in operating mode HAND
Display Setting range Operating function
Temperature set point
0.0 °C to 50.0 °C Must be at least 7 °C / 12.6°F above the ambient temperature in order to
ensure a stable temperature inside the chamber.

CO2 set point

0.0 vol.-% to 20.0
Common value: 5.0 vol.-% (factory setting), or depending on the NaHCO3
vol.-%
concentration of the culture medium (Figure 23 page 53).

Safety controller set point when set point type is “Limit” (Lit)
Limit value, i.e., maximum permitted absolute temperature value. When
0.0 °C to 60.0 °C exceeded, the safety controller triggers an alarm. For technical reasons,
the limit value should exceed the temperature set point by at least 2 °C.
Typical value: 39.0 °C / 102.2°F.
Or
Safety controller set point when set point type is “Offset” (OFS)
Offset value, i.e. maximum over temperature above the selected set point
0.1 K to 10 K
When exceeded, the safety controller triggers an alarm. For technical
reasons, the offset value should be at least 2 °C (factory setting).

Max. 3-digit numeric password for locking/unlocking the operating func-

tion setting in operating mode LOCK (chap. 7.4)
0 to 999
To permit temporary unlocking: select any password ≠ 0.
To permit permanent unlocking: set password to “0”.

The operating function “At.S” in operating mode USER (chap. 7.3) specifies the safety control-
ler set point type “Limit” (Lit) or “Offset” (OFS).
• With set point type “Limit” (Lit), operating function “AL.S” (safety controller set point as limit
value) is displayed in operating modes HAND and USER. You can set a limit value.
• With set point type “Offset” (OFS), operating function “Ot.S” (safety controller set point as
offset value) is displayed in operating modes HAND and USER. You can set an offset val-
ue.

With set point type “Limit”, set the safety controller each time the set point for the temperature
is changed. Set the safety controller set point approx. 2 °C above the controller temperature
set point.
Factory setting: set point type “Offset” with safety controller set point 2 °C.

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7.3 Operating mode USER: Advanced settings

• In Normal display, press “MODE” and “EXIT” simultaneously for 3 seconds to access the options for
selecting the chamber's operating modes.

Figure 18: Selecting the operating mode

• Press “▲”until the value “OP.U” (operating mode USER) appears in the upper display.
• Press “MODE”. The display to enter the password appears.
The operating mode USER is password-protected by a number. The password is preset to "1" in factory.
You can change the password in operating function “PA.U”.

Figure 19: Password request

• Use “▲” or “▼” to enter the password and press “MODE”.

• Use “MODE” to successively call up the operating functions.
• Use “▲” or “▼”to set the values of the operating functions.

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Operating functions in operating mode USER
Display Setting range Operating function
Temperature set point
0.0 °C to 50.0 °C Must be at least 7 °C / 12.6 °F above the ambient temperature in order to
ensure a stable temperature inside the chamber.

CO2 set point

0.0 vol.-% to 20.0
Common value: 5.0 vol.-% (factory setting), or depending on the NaHCO3
vol.-%
concentration of the culture medium (Figure 23 page 53).

Altitude of the installation site above sea level

0.0 km to 2.0 km For adjusting the displayed CO2 concentration depending on the air pres-
sure

Temperature alarm threshold

The maximum permitted deviation from the temperature set point in K,
0.1 K to 10.0 K
Deviations trigger a tolerance range alarm (chap. 10.5) on the main con-
troller. For technical reasons, the deviation should be at least 1.0K.

Temperature alarm-delay time

Period until the main controller triggers a tolerance range alarm after
closing the door. This prevents alarms constantly being triggered during
0 to 999 min
the unstable operating phase after closing the door.
This has no effect on the safety controller, which can still trigger a limit
alarm or offset alarm.

CO2 alarm threshold

0.1 vol.-% to 5.0 The maximum permitted deviation from the CO2 set point in vol.-%. Devi-
vol.-% ations trigger a tolerance range alarm on the main controller. For tech-
nical reasons, the value should be at least 1.0 vol.-%.

CO2 alarm - delay time

Period until the main controller triggers a tolerance range alarm after
0 to 999 min
closing the door. This prevents alarms constantly being triggered during
the unstable operating phase after closing the door.

Setting the offset value for door heating

Offset to the temperature set-point (inner chamber), standard setting 1.5
0.1 K to 10.0 K
K. You can use this value to increase (setting 1.0 K) or decrease (setting
2.0 K) inner chamber humidity.

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Display Setting range Operating function
Safety controller set point when set point type is “Limit” (Lit)
Limit value, i.e., maximum permitted absolute temperature value. When
0.0 °C to 60.0 °C exceeded, the safety controller triggers an alarm. For technical reasons,
the limit value should exceed the temperature set point by at least 2 °C.
Typical value: 39.0 °C / 102.2°F.
Or
Safety controller set point when set point type is “Offset” (OFS)
Offset value, i.e., maximum over temperature above the selected set
0.1 K to 10 K
point. When exceeded, the safety controller triggers an alarm. For tech-
nical reasons, the offset value should be at least 2 °C (factory setting).

Inhibit time. This operating function is not required to operate the cham-
----- ber.
Its value should always be zero.

Set point type for safety controller: “Limit” (Lit)

In operating modes USER and HAND you can define the limit value
(“Al.S”).

Set point type for safety controller: “Offset” (OFS)

In operating modes USER and HAND you can define the offset value
(“Ot.S”).

Max. 3-digit numeric password for locking/unlocking the operating func-

tion setting in operating mode LOCK (chap. 7.4)
0 to 999
To permit temporary unlocking: select any password ≠ 0.
To permit permanent unlocking: set password to “0”.

Password setting for access to operating mode USER

0 to 999 Remember the modified password or you will no longer be able to access
operating mode USER.

Setting the chamber address

1 to 254 Addressing serves for data transfer e.g. via the communication software
APT-COM™ 3 DataControlSystem. Do not change standard setting “1”.

Firmware revision of main controller for service / maintenance pur-

poses.

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Display Setting range Operating function
Firmware revision of safety controller for service / maintenance pur-
poses.

Data record (year) for service / maintenance purposes.

Data record (month) for service / maintenance purposes.

Data record (day) for service / maintenance purposes.

Data set version for service / maintenance purposes.

OFF, or -199,9 °C
Temperature adjustment serves to adjust the temperature sensor.
to +300 °C

OFF, or
-10.0 vol.-% to CO2 final value adjustment serves to adjust the CO2 sensor.
110.0 vol.-%

The operating function “At.S” specifies the safety controller set point type “Limit” (Lit) or “Off-
set” (OFS).
• With set point type “Limit” (Lit), operating function “AL.S” (safety controller set point as limit
value) is displayed in operating modes HAND and USER. You can set a limit value .
• With set point type “Offset” (OFS), operating function “Ot.S” (safety controller set point as
offset value) is displayed in operating modes HAND and USER. You can set an offset val-
ue.

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7.4 Operating mode LOCK: Locking/unlocking of the operating functions’ set-
tings by operating mode HAND
To avoid operating functions being changed by unauthorized persons, you can lock the operating func-
tions’ settings.
• In operating mode HAND, define a 3-digit numeric password in operating function “PA.H”.
Locking the operating functions of operating mode HAND:
• In Normal display, press “EXIT” and “MODE” simultaneously for 3 seconds to access operating mode
selection. In the upper display, “OP.H” (operating mode HAND) or “OP.U” (operating mode USER) is
displayed.
• Press “▼”. In the upper display, “OP.L” (operating mode LOCK) is now displayed. It is visible only if a
password was set in operating mode HAND via operating function “PA.H”.
• Confirm the selected operating mode with the “MODE” button. The controller returns to Normal dis-
play. Setting the operating functions in operating mode HAND is now locked.

Figure 20: Locking and unlocking the operating functions’ settings

Unlocking the operating functions of operating mode HAND:

• In Normal display, press “EXIT” and “MODE” simultaneously for 3 seconds to access operating mode
selection. In the upper display, “OP.L” (operating mode LOCK) is displayed.
• Press “▼” to enter operating mode HAND. In the upper display, “OP.H” (operating mode HAND) is
displayed.
• Press “MODE”. The menu to enter the password is displayed.

Figure 21: Password request

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• Enter the password using the “▲” and “▼”buttons.
• Confirm the entry with “MODE”. The controller returns to Normal display.
If you have selected any password other than zero, operating mode HAND is temporarily unlocked.
Changing the operating functions in operating mode HAND is now possible until a period of 30 sec. has
passed with no activity. 30 seconds after the last keypad entry, the operating functions are relocked (dis-
play “OP.L”).

To permanently unlock the settings, set operating function “PA.H” to “0” in HAND mode (chap.
7.2).

Display Setting range Operating function

Password request
Entry of the password that has been defined in operating mode HAND in
0 to 999
operating function “PA.H” (chap. 7.2). The operating functions’ settings in
operating mode HAND are temporarily unlocked.

7.5 Performance during and after power failures and shut down
In the event of power failure, all controller functions are taken out of operation. The gas inlet valves are
closed so that no gas can escape into the ambient air. The zero-voltage relay alarm output (13) (chap.
10.3) is switched to alarm position indicating the alarm for the whole duration of the power failure.
After the power returns or after turning on the chamber by hand, the incubator regulates the temperature
and CO2 to the last entered set points.
If the chamber was in sterilization mode, the process is cancelled and the chamber continues normal
operating mode with the original set points previously entered .

All settings and set point values remain in the memory during power failure after power off.

8. Controller RP1 settings

8.1 Altitude of the installation site above sea level
After first turning on the incubator, enter the altitude of the site above sea level into the controller RP1.
This entry serves to correct the calculation of CO2 concentration in vol.-% from the measurement of par-
tial pressure. The setting will remain stored after shutting the power off.
Procedure:

Normal Display

simultaneously for 3 seconds

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Toggle to operating mode USER
Operating mode HAND Operating mode USER

If operating mode HAND appears,

toggle to operating mode USER with
the arrow keys.

Password request

Password entry
(factory setting: “1”).

4x

Entry of altitude

Entry of the current altitude [km] above sea level with arrow keys.

After 30 seconds the controller reverts to Normal Display automatically.

Unit of altitude above sea level for entry and displayed value: kilometer [km]
Correlation feet [ft] to kilometer [km]: see chap. 19.5.

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8.2 Entering the set points of temperature and CO2

Normal Display

Entry of temperature set point

Entry of temperature set point with arrow keys.

Entry of CO2 set point

Entry of CO2 set point with arrow keys.

After 30 seconds the controller reverts to Normal Display automatically.

When setting a lower temperature set point, in order to save time, we recommend cooling
down the chamber by turning it off and opening both doors of the chamber.

When setting a lower CO2 set point, the CO2 gas must be able to escape first. Open both
doors of the chamber for this purpose.

If the CO2 concentration is indicated as “-0.4”, the CO2 sensor is defective. Please contact
BINDER Service.

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Note when setting high gas concentrations
Carbon dioxide (CO2) in high concentrations is hazardous to health. It is colorless and almost odorless
and therefore practically imperceptible. Any CO2 gas that may escape must be safely led out via good
room ventilation or a suitable connection to an exhaust system. We recommend installing a CO2 warning
system.

WARNING
High concentration of CO2 (> 4 Vol.-%).
Danger of death by suffocation.
Danger of poisoning.
∅ Do NOT set up chambers in non-ventilated recesses.
 Ensure technical ventilation measures.
 Observe the relevant regulations for handling CO2.

If CO2 is released, leave the area und inform the security service or fire department.

9. Temperature safety devices

9.1 Over temperature protective device (class 1)
The CO2 incubator is equipped with two internal temperature safety devices class 1 acc. to DIN 12880.
They serve to protect the chamber and prevent dangerous conditions caused by major defects.
If one of the over temperature protective devices permanently turns off the chamber, the user cannot
restart the device again. The protective cut-off devices are located internally. Only a service specialist can
replace them. Therefore, please contact an authorized service provider or BINDER Service.

9.2 Safety controller (temperature safety device class 3.1)

The incubator is equipped with an over temperature safety device class 3.1 acc. to DIN 12880. It is des-
ignated as the “safety controller”. This second, electrically independent temperature controller takes over
at a selectable set point in case of a faulty condition. It serves to protect the charging material against
extremely high temperatures.
In addition to the temperature set-point (main controller set-point), you can enter an independent safety
controller set-point. Example with setting “Limit”: set-point = 37 °C, safety controller set-point = 39 °C.
During proper operation, the controller controls temperature to the temperature set-point (37 °C). In the
event of a defect, which causes temperature to rise above the safety controller set-point of 39 °C, the
safety controller takes over and acts independently, limiting the temperature to 39 °C.
The message “otc” on the controller display indicates safety controller activity. The LED “ALARM” flash-
es, the LED “ ” is lit. At the same time there is an additional audible alert (buzzer). Pressing the “EXIT”
button turns off the audible alarm. When the temperature falls below the safety controller set-point (39
°C), the safety controller hands over to the main controller. Then the alarm message “otc” and the LEDs
“ALARM” and “ ” go off.

Regularly check the safety controller setting for set point type “Limit” or “Offset”.
Set the safety controller set point by approx. 2 °C above the desired temperature set point.

Safety controller set point types:

Limit Absolute maximum permitted temperature value

Example: temperature set point 37 °C / 98.6°F, safety controller set point 39 °C / 102.2°F
Offset Maximum over temperature above any active temperature set point (e.g., 2 °C). The maxi-
mum temperature changes internally and automatically with every set point change.

Factory setting: set point type “Offset” with safety controller set point 2 °C.

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 The settings of the safety controller are inactive during sterilization (chap. 15.4). They become
functional again following abortion of the sterilization and/or the restart of the chamber at the
main power switch.

9.2.1 Setting the safety controller set point type

Select the safety controller set point type in operating mode USER (chap. 7.3). The operating function
“At.S” specifies the safety controller set point type “Limit” (Lit) or “Offset” (OFS).
• With set point type “Limit” (Lit), operating function “AL.S” (safety controller set point as limit value) is
displayed in operating modes HAND and USER. You can set a limit value.
• With set point type “Offset” (OFS), operating function “Ot.S” (safety controller set point as offset value)
is displayed in operating modes HAND and USER. You can set an offset value.
Normal Display

simultaneously for 3 seconds

Toggle to operating mode USER

Operating mode HAND Operating mode USER

If operating mode HAND appears,

toggle to operating mode USER with
the arrow keys.

Password request

Password entry
(factory setting: “1”).

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12 x

Selecting the set point type

Selecting set point type “Limit” Selecting set point type “Offset”
Change using the
arrow keys

Select the desired set point type.

After 30 seconds the controller reverts to Normal Display automatically.

9.2.2 Setting the safety controller set point

You can check and set the safety controller set point in operating modes HAND (chap. 7.2) or USER
(chap. 7.3).
• With set point type “Limit” (Lit), operating function “AL.S” (safety controller set point as limit value) is
displayed in operating modes HAND and USER. You can set a limit value.
• With set point type “Offset” (OFS), operating function “Ot.S” (safety controller set point as offset value)
is displayed in operating modes HAND and USER. You can set an offset value.

The settings of the safety controller are inactive during sterilization (chap. 15.4). They become
functional again following abortion of the sterilization and/or the restart of the chamber at the
main power switch.

Normal Display

simultaneously for 3 seconds

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Toggle to operating mode USER
Operating mode HAND Operating mode USER

If operating mode HAND appears,

toggle to operating mode USER with
the arrow keys.

Password request

Password entry
(factory setting: “1”).

10 x

Depending on the selected set point type (chap. 9.2.1) a different display appears:
Entry of limit value or offset value
With set point type Limit: With set point type Offset:
Enter a limit value Enter an offset value

or

Entry of limit value using the arrow keys. Entry of offset value using the arrow keys.

After 30 seconds the controller reverts to Normal Display automatically.

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10. Alarm functions
10.1 Alarm functions overview
When operational faults occur, the controller triggers visual and audible alarm signals. The LED “ALARM”
always flashes when an alarm signal is emitted.
A zero-voltage relay alarm output (13) (chap. 10.3) permits transmission of the alarm e.g., to a central
monitoring system.

Display Event

Safety controller alarm (set value of the safety controller exceeded)

Temperature tolerance range alarm

Door open

CO2 tolerance range alarm

CO2 pressure too low

Failure of temperature sensor for door heating

Failure of temperature sensor for safety controller

Failure of temperature sensor for interior heating

CO2 sensor not connected

Sterilization program started with operating mode HAND locked (LOCK)

or
Sterilization program started with the CO2 sensor still plugged-in.

If more than one alarm signal is sent simultaneously, they are displayed in a cycle, except for alarms 995
to 999. These take priority over all other operational displays and alarm signals of the controller.
Except for the tolerance range alarms, all alarms are displayed immediately when the fault occurs. The
tolerance range alarms are suppressed for a selected time (delay times set for temperature alarm and for
CO2 alarm) after opening the chamber door or turning on the chamber.

10.2 Resetting the alarm messages

• Remove the cause of the alarm or wait until the chamber compensates for the reason of the error.
• The visual alarm disappears, when the cause of the fault has been remedied or the monitored operat-
ing function returns within its tolerance limits.
• Press the “EXIT” button to turn off the audible alarm signals.

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10.3 Zero-voltage relay alarm output
Collective alarm output via the zero-voltage relay alarm contact
The CO2 incubator is equipped at the rear with a zero-voltage relay output
for temperature and CO2, which permits the transmission of alarms to an
external monitoring system in order to monitor and record the alarm signals.

A DIN socket (15) serves to establish this connection.

Figure 22: Pin configuration of the DIN socket (15)

Pin 1: Pole
ALARM Pin 2: Break relay
Pin 3: Make contact

In case there is no alarm, contact 1 closes with contact 3.

Closing contact 1 with contact 2 switches the zero-voltage relay alarm output.
The zero-voltage relay alarm output switches immediately, as soon as the red “ALARM” LED lights up on
the controller display. The zero-voltage relay alarm output switches for alarm instances listed in chap.
10.1 and in case of power failure.
Maximum loading capacity of the switching contacts: 24V AC/DC – 2.5 Amp.

DANGER
Electrical hazard.
Danger of death.
Damage to switching contacts and connection socket.
∅ Do NOT exceed the maximum switching load of 24V AC/DC – 2.5 Amp.
∅ Do NOT connect any devices with a higher loading capacity.

The alarm message on the controller display remains displayed during transmission of an alarm via the
zero-voltage relay outputs.
As soon as the cause of the alarm is identified and resolved, the alarm transmission via the zero-voltage
relay outputs resets automatically together with the alarm message on the controller display.
In case of a power failure, transmission of the alarm via zero-voltage relay outputs remains active for the
duration of the power failure. Afterwards, contact 1 will close automatically with contact 3.
Connection to an external monitoring system
To ensure short-circuit-proof alarm monitoring that will trigger the alarm when the chamber is connected
to an external alarm monitor, connect the external alarm monitoring system to the chamber via the con-
tacts 1 and 3.

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10.4 Safety controller temperature alarm
The set temperature value (set point type “Limit” or “Offset”) was exceeded.
• Immediate alarm
• Visual display
LED Upper display, alternating
Temperature Alarm code
Flashes
continually lit

• Audible alarm: buzzer (ongoing sound)

• Switching the zero-voltage relay alarm output.
Actions:
• Check the setting of the operating function “Al.S” (limit temperature) or “Ot.S” (offset temperature) in
operating modes HAND (chap. 7.2) or USER (chap. 7.3). The limit temperature should be at least 2 °C
above the temperature set point; the offset temperature should be ≥ 2 °C. If necessary, adjust the rele-
vant value.
• Check whether samples were inserted into the chamber that produce heat under the climatic condi-
tions in the chamber.
• Check the ambient conditions. The ambient temperature must be at least by 7 °C / 12.6°F below the
temperature set point of the chamber. Protect the chamber from direct sunlight. Ensure sufficient venti-
lation around the installation location to prevent any buildup of heat in the chamber.
• Check whether the sterilization cycle was aborted and the chamber has returned to its standard opera-
tional status before the chamber has cooled down.
• If points 1 to 4 do not reveal the source of the fault, it may be that the chamber is faulty. Please contact
BINDER Service.

10.5 Temperature tolerance range alarm (high and low temperature)

Main controller temperature alarm: The temperature has risen above or fallen below the temperature
alarm threshold.
• Immediate alarm

No alarm signal is emitted during the temperature delay time after the outer door is closed

• Visual display
LED Upper display, alternating
Temperature Alarm code
Flashes
Flashes

• Audible alarm: buzzer (intermittent sound)

• Switching the zero-voltage relay alarm output
Actions:
• Check the setting of the operating function “AL.1” (temperature alarm threshold) in operating mode
USER (chap. 7.3). The value should be ≥ 1K. Adjust the value if necessary.
• Use the displayed temperature to verify whether the temperature alarm threshold has been breached,
i.e., too cold or too warm.

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Temperature too low (under temperature alarm):
• Check that the outer door is closed properly.
• Check the door's seals for any damage. Replace any damaged seals.
Temperature too high (over temperature alarm):
• Check whether samples were inserted into the chamber that produce heat under the climate condi-
tions in the chamber.
• Check the ambient conditions.
The ambient temperature must be at least by 7 °C / 12.6 °F below the temperature set point of the
chamber. Protect the chamber from direct sunlight. Ensure sufficient ventilation around the installation
location to prevent any buildup of heat in the chamber.
• If points 1 to 6 do not reveal the source of the fault, it may be that the chamber is faulty. Please contact
BINDER Service.
• To decrease the temperature, proceed as follows: Turn off the chamber. Open both chamber doors for
approx. 5 minutes. Turn on the chamber again. You can restart normal operation as soon as the re-
quested values have equilibrated.

If the same alarm recurs, please contact BINDER Service.

10.6 Door open

The open and closed position of the chamber door is controlled via the door contact switch. If the door is
open, the temperature and CO2 controls turn off.
• Immediate alarm
• Visual display:
LED Upper display, alternating
Temperature Alarm code

flashes

• Audible alarm: buzzer (intermittent sound, beginning 5 minutes after the door is opened)
• Switching the zero-voltage relay alarm output (beginning 5 minutes after the door is opened)
Actions:
• Close the outer door.
• Use the “EXIT” button to turn off the buzzer even when the door is open.
• The alarm message is cancelled.
• The zero-voltage relay alarm output switches off.

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10.7 CO2 tolerance range alarm (CO2 over/under concentration)
The main controller CO2 alarm.
The CO2 concentration has risen above or fallen below the CO2 alarm threshold.
• The alarm signal is usually emitted immediately upon the fault occurring, but no alarm signal is emitted
during the CO2 alarm delay time after the outer door is closed.
• Visual display:
LED Lower display, alternating
Percentage CO2 Alarm code
Flashes
Flashes

• Audible alarm: buzzer (intermittent sound)

• Switching the zero-voltage relay alarm output
Actions:
• Check the setting of the operating function “AL.2” (CO2 alarm-threshold). The value should be ≥ 1 vol.-
% CO2. If necessary, adjust the value.
• Check that the outer door is closed properly.
• Check the door's seals for any damage. Replace any damaged seals.
• If the above points do not reveal the source of the fault, it may be that the chamber is faulty. Please
contact BINDER Service.
In case of a CO2 over concentration alarm, proceed as follows:
• Open both chamber doors for approx. 30 seconds. Respect the precautions when working with CO2
gas (chap. 1.6).
• Normal operation can be restarted, as soon as the requested values have been readjusted.

If the same alarm recurs, please contact BINDER Service.

10.8 CO2 pressure too low

The CO2 primary pressure at the intake valve is less than 0.3 bar / 4.4 psi below the ambient air pressure.
The alarm displays show a pressure drop of CO2 admission below 0.3 bar / 4.4 psi. Check whether the
gas cylinder is open.
• Immediate alarm
• Visual display:
LED Lower display, alternating
Percentage CO2 Alarm code
Flashes
Flashes

• Audible alarm: buzzer (intermittent sound)

• Switching the zero-voltage relay alarm output

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Actions:
• Check that you have set the pressure on the pressure reducer at 2.0 bar / 29 psi above the ambient
air pressure, and that all the valves are open for the gas supply.
• Where the CO2 supply is a pressurized gas cylinder, check that the cylinder still contains sufficient
CO2. If necessary, replace the gas cylinder. Observe the precautions when handling gases and the
correct outlet pressure (chap. 4.4).
• Check whether the primary pressure is high enough at the central CO2 supply.
• Check that the gas tube has no damage, kinks, blockages or soiling.
• Check when the gas filter was last replaced. Replace the gas filter every year to avoid it blocking.
A qualified service engineer should replace the gas filter.
• If points 1 to 5 do not reveal the source of the fault, it may be that the chamber is faulty. Please contact
BINDER Service.
The outlet pressure of the gas cylinder must be 2.0 bar / 29 psi above the ambient pressure.

CAUTION
Excessive outlet pressure > 2.5 bar / 36 psi.
Damage to the chamber.
∅ The outlet pressure must NOT exceed the indicated value of 2.5 bar / 36 psi.
 Before connecting, check the outlet pressure on the pressure reducer of the cylinder.
 Adjust the cylinder outlet pressure to 2.0 bar / 29 psi above the ambient pressure.

The recovery times of the gas concentrations inside the chamber following the door being opened, which
are indicated in the technical data (chap. 19.4), refer to a connection pressure of 2.0 bar / 29 psi. De-
creasing supply pressure down to the alarm point of 0.3 bar / 4.4 psi results in longer recovery times.
Check the pressure displays of your gas supply. If very short recovery times are required or the door is
opened frequently, replace the gas cylinders promptly when the pressure decreases below 2.0 bar / 29
psi.

10.9 Temperature sensor failure

A sensor fault alarm display takes priority over all other operational displays and alarm signals on the
controller.
• Immediate alarm
• Visual display:
Upper Display, flashing
LED Meaning
Alarm code
Failure of temperature sensor for interior heating:
interior heater is turned off

Failure of the safety controller's temperature sen-

Flashes
sor: all heaters are turned off

Failure of temperature sensor for door heating:

door heater is turned off

• Audible alarm: buzzer (intermittent sound)

• Switching the zero-voltage relay alarm output
Actions:
• Turn off the chamber.
• If necessary, clean and disinfect the chamber. Automatic sterilization is not possible with this fault.
• Please contact BINDER Service.

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10.10 Failure of CO2 sensor
If the CO2 concentration is indicated as “-0.4”, the CO2 sensor is defective (sensor rupture).
Normalanzeige

Actions:
• Turn off the chamber.
• If necessary, clean, disinfect and sterilize the chamber.
• Please contact BINDER Service.

11. Error messages

While the controller is operating, or when you turn it on or change the operation mode, fault signals may
be emitted that are caused by the controller malfunctioning internally.
Visual display (examples):
LED Upper display Lower display

Flashes

Flashes

Flashes

Flashes

Actions:
• Turn off the chamber with the main power switch.
• Disconnect the power plug.
• Wait for 1 minute.
• Replace the power plug.
• Turn on the chamber with the main power switch.
• If the fault shows again, the chamber could be faulty. Please contact BINDER Service.

Repair must only be performed by qualified service personnel authorized by BINDER.

Repaired chambers must comply with the BINDER quality standards.

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12. Reference measurements
You can take reference measurements of the temperature and CO2 via the silicone measuring port (8)
located the inner glass door. Reference temperature measurements always take place under equilibrated
conditions with both doors closed.

12.1 CO2 reference measuring

There are three possibilities to perform CO2 test measurements between the recommended annual
maintenance procedures. To test the CO2 concentration inside an incubator, see chapters 12.1.1 to
12.1.3.

12.1.1 Measuring the CO2 concentration indirectly via the pH of the cell medium

By using the indirect determination of CO2 concentration via the pH-value of the nutrient, it is possible to
check the CO2 concentration inside the chamber. This is a simple method to test the correct CO2 concen-
tration without any special CO2 measuring equipment. You need only use an accurate pH indicator or a
pH-measuring electrode, which are standard equipment in cell culture laboratories.

This method is not suitable for calibrating the BINDER FPI sensor system.

This method is based on the acid base equilibrium of the buffer system in the culture media.
NaHCO3 buffers the common media. From the pH value of the medium, it is possible to conclude its CO2
concentration. Figure 23 shows the relationship between CO2 concentration in vol.-% and the pH of dif-
ferent NaHCO3 buffered media.
Recommended procedure:
• Incubate an empty sample with medium for 1/2 day under the same conditions as the cells. You can
perform the incubation in a cell culture bottle or in a 50 ml Falcon tube with open lid.
• After gassing, remove the empty sample from the incubator and within 5 minutes measure the pH-
value with a glass electrode.
During the measurement, the medium should have the least possible surface contact with the ambient
air, so that the CO2 can evaporate only slightly. A significant downward movement will happen only af-
ter 5 minutes, permitting sufficient time for measurement.
• In addition, you can of course use pH-test strips (pH range 6 to 8, non-bleeding).

Vol.% CO2
18
Trade names of common media:
3,70 g/l NaHCO3
16 NaHCO3 [g/l]
14 DMEM 3.70
2,20 g/l NaHCO3 BME 2.20
12
MEM 2.20
10 Medium 199 2.20
1,20 g/l NaHCO3 Mc Coy 2.20
8
F10 1.20
6 F12 1.20
0,85 g/l NaHCO3
4

2 0,35 g/l NaHCO3

6.8 7.0 7.2 7.4 7.6

Value pH
Figure 23: Value pH of NaHCO3 buffered media as a function of the CO2 concentration:

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Example:
If a pH of 7.2 is measured in a medium buffered with 2.20 g NaHC03 per liter, there must be 8 vol.-% CO2
surrounding this medium.

12.1.2 Measuring CO2 directly via chemical indicator tubes

This is a common do-it-yourself test for many users. A chemical color reaction in a glass tube shows the
CO2 concentration. A standardized volume of air from inside the incubator has to be suctioned through
this glass tube to get a quantitative test result. Therefore, use a special hand pump with a standardized
suction volume.
Procedure (example):
(1) Break off both ends of the glass tube or remove the plugs.
(2) Pin the end with the higher end of the scale to the adapter of the hand pump that belongs to that
test system.
(3) Pin the other end through the silicone access port of the inner chamber door.
(4) Take one sample volume out of the inner chamber volume by pressing the pump fully together and
releasing it afterwards.
(5) The standardized volume is suctioned through the glass tube and the chemical indicator changes
its color beginning from the side pinned into the chamber in the direction of the hand pump.
(6) The more CO2 is inside the chamber the more the chemical reaction will cause a color change of
the chemical reactor.
(7) You can read the CO2 concentration by the scale directly printed on the glass tube or a delivered
reference-reading rule.
(8) The result must then be corrected to the current ambient pressure. The required formula is printed
on the instruction sheet of such systems.
All the necessary equipment must be supplied by one manufacturer only and in a defined test system.
Note: These test systems are not very accurate. A typical accuracy is around 10% of the full-scale value.

These test systems are not suitable for calibrating the BINDER FPI sensor system.

Figure 24: Example of chemical indicator Figure 25: Example of a hand pump (foreground) and elec-
tubes trical pump (background)

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12.1.3 Measuring CO2 directly with an electronic infrared measuring device

The easiest way of measuring the CO2 concentration is by electronic sensor systems. BINDER offers the
portable measuring device model CTM 01 that was specifically designed to measure temperature and
CO2 concentration inside CO2 incubators. The CTM 01 is suitable both for reference measurements in
certified laboratories, and for service purposes. Please contact the BINDER INDIVIDUAL team.

12.2 Temperature reference measurement

When performing a temperature reference measurement using an electronic measuring, and temperature
display device, it is important to use a device traceable to an acknowledged standards/calibration institu-
tion (DKD, PTB for Germany) with valid calibration certificate.
Note: The cable of the sensor must be thin enough to lay it over the door gasket of the incubator without
causing any leakages.

13. Options
13.1 Silicone access ports 30 mm / 1.18 in, closable with 2 silicone plugs
(8012-0558 rear, 8012-0559 left, 8012-0560 right) (option)

(c)
(a)

(b)

Figure 26: Positions of the optional silicon access ports left (a), rear (b), and right (c)

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A warning sticker is located above each access port.
When operating a chamber with silicon access ports, both silicon plugs must tightly close the access
ports. If the plugs are inserted in a not-gastight manner, or if plugs are missing, CO2 gas may escape into
the environment. The CO2 control only turns off when the chamber door is opened.

WARNING
High concentration of CO2 (> 4 Vol.-%).
Risk of death by suffocation.
Danger of poisoning.
 Tightly close each access port with two plugs during operation.

13.2 Base on castors (option)

In order to obtain easy access to the incubator and to avoid contamination of the incubator caused by soil
pollution, BINDER recommends using the base on castors.

The mounting instructions 7001-0147 delivered with the base on castors describe its
installation (Art. No. 9051-0024).

13.3 Stacking adapter for direct thermal decoupled stacking (option)

We recommend not stacking CO2 incubators directly on top of one another in order to avoid transmission
of shocks and vibrations from one chamber to the other. This could happen e.g. while opening or closing
the door, cleaning, loading and unloading the chamber. BINDER offers a stacking adapter for direct ther-
mal decoupled stacking of two incubators.
The stacking adapter ensures the exact maintenance of the set incubation parameters also during sterili-
zation of the other chamber in the same stacking stand (chap. 15.4).

The mounting instructions 7001-0145 delivered with the stacking adapter describe its
installation (Art. No. 9051-0025 for 2 C 170, Art. No. 9051-0027 for C 170 on top of CB
150/160).

13.1 Analog outputs for temperature and CO2 (option)

With this option, the CO2 incubator is equipped with analog outputs 4-20 mA for temperature and CO2.
These outputs allow transmitting data to external data registration systems or devices.
The connection is realized as a DIN socket at the rear of the CO2 incubator as follows:
ANALOG OUTPUT 4-20 mA DC
PIN 1: Temperature –
PIN 2: Temperature +'
PIN 3: CO2 –
PIN 4: CO2 +
CO2 range: 0 vol.-% up to 20 vol.-%
Temperature range: 0 °C / 32°F up to +200 °C / 392°F
A suitable DIN plug is enclosed.
Figure 27: Pin allocation of DIN socket for option analog outputs

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14. Avoiding microbial contamination
The main types of microbial contaminants in cell and tissue culture are bacteria, fungi, yeast, mycoplas-
ma, and viruses. This chapter gives an overview of potential sources of contamination and precautions
and measures to eliminate them.

14.1 Cells and media

• Primary cultures from the original tissue

• Cells/cell lines from unknown sources or from cell banks: Use only cells of known and tested origin.
Monitoring and routine screening of new cultures.
• Media and sera: Use only sera of known and tested origin (mycoplasma free, e.g., UV or γ radiated).
• Virus suspensions, antibody solutions etc. Use only reagents of known and tested origin.
• Laboratory instruments, media and reagents, which were exposed to possible contaminated cultures
must be sterilized / autoclaved / disposed.
• Antibiotics in the cell culture media may prevent bacteria detection: Use antibiotics selectively and
economically.

14.2 Laboratory conditions / equipment around the incubator

Possible sources of contamination in the cell culture lab are airborne germs, lab equipment, building fea-
tures, and the lab personnel.
• Keep pipettes and instruments sterile after autoclaving.
• Bio safety cabinets (laminar air flow) should have a minimum of items apart from aspirator tube and
burner. Items shall be positioned within easy reach and separate from each other. Disinfect surfaces
with an alcohol-based disinfectant before and after use, clean the space underneath the bench, and
carry out regular sterility tests of the filters.
• Regular cleaning / disinfection of laboratory equipment such as a centrifuge, microscope, water bath,
refrigerator, and telephone.
• No equipment should be placed on the floor.
• Rough or humid walls are not suitable.
• Identify leaking doors and windows and make them airtight.
• Use air conditioning with special filters.
• Reduce the number of personnel and their movements in the lab by careful positioning all relevant
equipment. For practical reasons, install the CO2 incubator close to the laminar air flow bench.
• Regular microbiological monitoring of the cell culture laboratory.

14.3 Working and behavior in the lab

Sources of contamination are often the laboratory personnel themselves (surface germs, oral flora droplet
transfer) and handling the equipment and cultures. We recommend staff training in aseptic techniques,
laboratory safety and good laboratory practice (GLP).

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Examples of general rules to reduce the contamination risks
• Reduce hand germ count (wash hands with antimicrobial soap, dry with paper tissues, and rub dry
hands with alcoholic solution).
• Wear appropriate clothing (work coat, shoes, face mask)
• Keep as few personnel as possible in the cell culture lab.
Examples of sterile working method
• Work "clean-to-dirty", i.e., handle confirmed uncontaminated cells first, unknown or untested cells next,
and lastly, if necessary, cells suspected to be contaminated.
• Perform daily microscopic observations of cultures and specific tests for the bacteria and fungi as part
of a controlled routine. Test cultures for sterility before starting work.
• Keep working surfaces clean. Immediately wipe spilled liquids with alcohol solutions.
• No mouth contact on pipettes.
• Never work on top of open sterile containers.

14.4 Chamber design and equipment

The design concepts behind the CO2 incubator C 170 considerably reduce the risk of contamination.
Among them are:
Even surfaces for easy manual cleaning
• The inner surfaces are smooth and therefore easy to clean. The inner chamber is deep-drawn from a
single piece of steel, polished (suitable for pharmaceutical work) and has no welds or inaccessible
corners. The hinges and the seal of the inner glass door are glued from the outside, which also aids
cleaning of the inner chamber.
Removable parts for cleaning and autoclaving
• The shelves and the shelf holder are easily removed without screws. In this way, you can clean them
hygienically in every laboratory dishwasher before hot-air sterilization. It is possible to autoclave the
shelves. But this is generally not necessary because they can remain inside the incubator during steri-
lization.
Door gasket
• The inner door gasket is removable and autoclavable.
Gas fine filter
• The incoming gas used in the operation passes through a fine filter (aseptic filter, filtration efficiency
99.99%, particle size 0.45 µm) with a high filtration efficiency, which can also filter the smallest parti-
cles (chap. 16.3).
CO2 measuring system in the inner chamber
• You can remove the CO2 sensor from the inner chamber by hand for disinfection (chap. 15.3).
Condensation prevention
• Condensation in the inner chamber represents a particular risk of contamination. The humidifying sys-
tem with water pan with integrated condensation point developed by BINDER is an effective and easy
way to ensure high humidity (95 ± 2% r.H.) inside the incubator without any condensation forming on
the inner surfaces.
Hot air sterilization at 180 °C / 356 °F
• The heating system of the chamber permits hot-air auto-sterilization (chap. 15.4). Thus, a temperature
of 180 °C / 356°F is maintained for a 30-minute period on all internal surfaces during its cycle, result-
ing in sterilization of the entire inner chamber. Therefore, this procedure meets all international guide-
lines regarding hot air sterilization, e.g. AAMI ST63, DIN 58947, European Pharmacopoeia.

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14.5 Handling the CO2 incubator
Any manipulation of the CO2 incubator involves some contamination risks, from installation to opening of
the doors and regular cleaning.
Installation away from sources of contamination
• Do not place the CO2 incubator on the floor or close to windows and doors. Use the optional stand, if
appropriate.
Reduce the periods in which the door is open.
• Do not open the door too frequently.
• Placing items in order inside the incubator results in shorter door opening times.
Water pan
• Fill the water pan with distilled, sterilized water (chap. 4.3). Never use ion exchange water; ion ex-
changers are propagation sites for bacteria.
• Clean and refill the pans 2 to 3 times a week. For evacuation, remove the water pan. It is autoclava-
ble.
• If desired, you can add microbiologically inhibiting substances such as copper chips, copper sulphate
or ethylene diamine tetra-vinegar acid (EDTA) in a concentration of 1 to 5 mmol/l.
Avoiding condensation caused by ambient conditions
• Ambient room conditions have an effect on condensation inside the incubator, which can be caused by
insufficient wall clearances, preventing even dissipation of heat, air movement or direct sunlight. If the
temperature distribution inside the chamber becomes uneven, condensation may form on the cooler
surfaces.
• Maintain distances from the wall: rear 100 mm / 3.94 in, sides 50 mm / 1.97 in.
• Do not place the chamber in front of a window. No direct sunlight. No air movement.
• Permissible ambient temperature range for operation: +18 °C / 64.4°F to +30 °C / 86°F. Ideal ambient
temperature: at least 7 degrees below the intended working temperature. E.g., working temperature
37 °C / 98.6°F = ambient temperature 30 °C / 86°F and less.
• The incubator should be precisely calibrated / adjusted.
Regular cleaning, decontamination, and sterilization
• Clean the shelves, glass door, gaskets, and inner chamber weekly (for cleaning see chap. 15.1, for
decontamination see chap. 15.2). You can clean the shelves in a laboratory dishwasher and, if need-
ed, individually autoclave them.
• Regularly use the hot air sterilization function (chap. 15.4) following cleaning. Shelves, shelf holders
and the emptied water pan can remain inside the incubator during this operation.
• Replace the CO2 sterile filter (once or twice a year depending on usage).
What to do in case of contamination?
• Throw away / autoclave contaminated cultures.
• Carefully inspect cultures that seem to be uncontaminated.
• Clean the incubator as described. Wipe the inner chamber and the doors with a disinfectant and allow
to dry. Autoclave the shelves. Empty the water pan and autoclave it.
• Perform hot air sterilization.

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15. Cleaning, decontamination / disinfection, and sterilization
Clean the chamber after each use to avoid potential corrosion damage by ingredients of the charging
material.

DANGER
Electrical hazard.
Danger of death.
∅ Do NOT spill water or cleaning agents over the inner and outer surfaces.
 Before cleaning, turn off the chamber at the main power switch (2) and
disconnect the power plug.
 Completely dry the appliance before turning it on again.

15.1 Cleaning
Disconnect the CO2 incubator from the power supply before cleaning. Disconnect the power plug.

The interior of the chamber must be kept clean. Thoroughly remove any residues of the charg-
ing material.

Wipe the surfaces with a moistened towel. In addition, you can use the following cleaning agents:

Standard commercial cleaning detergents free from acid or halides.

Exterior surfaces,
Alcohol-based solutions.
instrument panel:
We recommend using the neutral cleaning agent Art. No. 1002-0016.
Inner chamber, Standard commercial cleaning detergents free from acid or halides.
shelf holders,
Copper sulphate solutions or alcohol-based solutions.
shelves,
water pan: We recommend using the neutral cleaning agent Art. No. 1002-0016.

Alcohol-based solutions
Do not immerse the CO2 sensor into the solution.
CO2 sensor Disinfection with alcohol or an alcohol-based surface disinfectant without corro-
sive effect, free from acid or halides. We recommend using the disinfectant spray
Art. No. 1002-0022.
Silicone door gasket: Alcohol-based solutions or neutral cleaning agent Art. No. 1002-0016.
Zinc coated hinge
Standard commercial cleaning detergents free from acid or halides. Do NOT use
parts, rear chamber
a neutral cleaning agent on zinc coated surfaces.
wall

Do not use cleaning agents that may cause a hazard due to reaction with components of the device or
the charging material. If there is doubt regarding the suitability of cleaning products, please contact
BINDER service.

We recommend using the neutral cleaning agent Art. No. 1002-0016 for a thorough cleaning.
Any corrosive damage that may arise following use of other cleaning agents is excluded from
liability by BINDER GmbH.

CAUTION
Danger of corrosion.
Damage to the chamber.
∅ Do NOT use acidic or chlorine cleaning detergents.
∅ Do NOT use the neutral cleaning agent on other kind of surfaces e.g., the zinc coated
hinge parts or the rear chamber wall.

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 For surface protection, perform cleaning as quickly as possible.
After cleaning completely, remove any cleaning agents from the surfaces with a moistened
towel. Let the chamber dry.

Soapsuds may contain chlorides and must therefore NOT be used for cleaning.

With every cleaning method, always use adequate personal safety controls.

Following cleaning, leave the chamber door open or remove the access port plugs.

The neutral cleaning agent may cause health problems in contact with skin and if ingested.
Follow the operating instructions and safety hints labeled on the bottle of the neutral clean-
ing agent.

Recommended precautions: To protect the eyes use sealed protective goggles. Suitable protective
gloves with full contact: butyl or nitrile rubber, penetration time >480 minutes.

CAUTION
Contact with skin, ingestion.
Skin and eye damage due to chemical burns.
∅ Do not ingest. Keep away from food and beverages.
∅ Do NOT empty into drains.
 Wear protective gloves and goggles.
 Avoid skin contact.

Following use of the neutral cleaning agent and prior to hot-air sterilization, remove any agent
residues by using a moistened towel in order to avoid formation of permanent residues.

15.2 Decontamination / chemical disinfection of the CO2 incubator

The operator must ensure that proper decontamination is performed in case a contamination of the
chamber by hazardous substances has occurred.
Disconnect the CO2 incubator from the power supply prior to chemical decontamination / disinfection. Pull
the power plug.
Do not use decontamination agents that may cause a hazard due to reaction with components of the
device or the charging material. If there is doubt regarding the suitability of cleaning products, please
contact BINDER service.
You can use the following disinfectants:

Standard commercial surface disinfectants free from acid or halides.

Inner chamber Alcohol-based solutions.
We recommend using the disinfectant spray Art. No. 1002-0022.

For chemical disinfection, we recommend the disinfectant spray Art. No. 1002-0022.
Any corrosive damage that may arise following use of other disinfectants is excluded from
liability by BINDER GmbH.

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 With every decontamination / disinfection method, always use adequate personal safety con-
trols.

In case of contamination of the interior by biologically or chemically hazardous material, there are two
possible procedures depending on the type of contamination and charging material.
1. Spray the inner chamber with an appropriate disinfectant.
Before start-up, the chamber must be absolutely dry and ventilated, as explosive gases may form dur-
ing the decontamination process.
2. You can sterilize the shelves and the shelf holder in a sterilizer or autoclave.

In case of eye contact, the disinfectant spray may cause eye damage due to chemical
burns. Follow the operating instructions and safety hints labeled on the bottle of the disin-
fectant spray.

Recommended precautions: To protect the eyes use sealed protective goggles.

VORSICHT
Eye contact.
Eye damage due to chemical burns.
∅ Do NOT empty into drains.
 Wear protective goggles.

Following frequent use of the disinfectant spray and prior to hot-air sterilization, remove any
agent remainder by using the neutral cleaning agent and then a moistened towel to avoid for-
mation of permanent residues.

After using the disinfectant spray, allow the incubator to dry thoroughly, and aerate it suffi-
ciently.

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15.3 Disinfection of the CO2 sensor
To ensure complete disinfection and proper function of the sensor, BINDER recommends a wipe disinfec-
tion of the sensor head with pure alcohol or non-corrosive alcohol-based surface disinfectants. The disin-
fectant must be non-corrosive and free of chlorine or any acid. We recommend using the disinfectant Art.
No. 1002-0022. Avoid strong shocks when handling the CO2 sensor.

CAUTION
Excess temperature.
Immersion of sensor into liquids.
Shocks of the CO2 sensor.
Damage to the CO2 sensor.
∅ Do NOT immerse the CO2 sensor into liquids.
∅ Do NOT expose the CO2 sensor to autoclaving.
∅ Do NOT expose the CO2 sensor to hot-air sterilization.
 Avoid strong shocks of the CO2 sensor (by putting it down hard, or dropping).

We recommend regular disinfection of the CO2 sensor.

CAUTION
Connecting or removing the CO2 sensor during operation.
Damage to the CO2 sensor.
 Connect or remove the CO2 sensor only with the chamber turned off.

Recommended procedure:
• Turn off the chamber
• Pull out the sensor
• Spray the sensor head with alcohol or wipe it clean with a soaked cloth. Observe the reaction time of
the disinfectant used.
• Before reinserting the CO2 sensor, it must be completely dry.
• The filter in the front of the sensor only needs replacing when damaged or dirty.
The CO2 sensor head was especially adjusted for the specific chamber. To avoid confusion, an adhesive
label with a serial number is adhered to the sensor head. When exchanging the sensor, repeat CO2 ad-
justment.

CAUTION
Different CO2 sensor.
Invalid calibration.
∅ Do NOT change the CO2 sensor head.
 Write down the serial number of the CO2 sensor.

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15.4 Hot-air sterilization at 180 °C / 356 °F

The very first sterilization after operation may cause an odor. This is not a quality defect. We
recommend ventilating well the room during sterilization.

15.4.1 Overview

Heating up Holding Cooling down

190 °C

37 °C

approx. 2
4 hours approx. 6 hours
hours
Sterilization cycle of approx. 12 hours

Figure 28: Set-point profile during the sterilization cycle

The chamber can perform an automatically controlled hot-air sterilization cycle. This procedure will take
approx. 12 hours and consists of the following steps:
• Heating up phase: The incubator heats up the inner chamber as fast as possible to the sterilization
set-point temperature
• Holding phase: Constant sterilization set-point temperature
The sterilization set-point temperature is pre-set in factory to 190 °C / 374 °F. When this temperature
is reached, the holding phase begins. The duration of the holding phase is in total 4 hours. This en-
sures that 180 °C / 356 °F is maintained on all internal surfaces for at least 30 minutes.
• Cooling down phase: until 37 °C / 98.6°F is reached.
When 37 °C / 98.6 °F ± 1 ° is reached, the display "End" in the lower controller display (Figure 32 page
67) indicates the end of the sterilization procedure.
The precise duration of the entire sterilization cycle depends on the ambient temperature at the installa-
tion site and can thus vary. At an ambient temperature of 25 °C / 77 °F, the total duration is approx. 12
hours. You can shorten the cooling-down time by aborting the sterilization cycle (chap. 15.5) during the
cooling-down phase, i.e. no sooner than after 6 hours.
During sterilization, the CO2 valve is closed and the CO2 controller turns off entirely.

15.4.2 Procedure for hot-air sterilization:

Before carrying out the first hot-air sterilization, remove any protective lamination sheet from
the inner metal surfaces.

When starting a hot-air sterilization, CO2 control automatically becomes inactive.

The safety controller settings are inactive during sterilization. They become functional again
following abortion of the sterilization and/or restart of the chamber at the main power switch.

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Recommended procedure:
• Turn off the chamber
• Pull out the CO2 sensor without any rotation from the connection socket in the upper part of the rear
and remove it from the inner chamber.
The CO2 sensor is temperature resistant up to a maximum temperature of 60 °C / 140 °F. It is
therefore required to remove it before performing a hot-air sterilization.

CAUTION
Excess temperature.
Damage to the CO2 sensor.
∅ Do NOT expose the CO2 sensor to hot-air sterilization.

The CO2 sensor head was especially adjusted for your specific chamber. To avoid confusion, an ad-
hesive label with a serial number is adhered to the sensor head. When exchanging the sensor, repeat
CO2 control adjustment.

CAUTION
Different CO2 sensor.
Invalid calibration.
∅ Do NOT change the CO2 sensor head.
 Write to down the serial number of the CO2 sensor.

• Empty the water pan.

WARNING
Danger of implosion.
Damage to the chamber.
 Empty the water pan before starting the hot-air sterilization.

• Clean the chamber.

• Water pan, shelf holder, and shelves must be inside the incubator, the water pan at its usual place on
the bottom.
• Close the inner glass door and the outer chamber door.
• Turn on the chamber.
• Activate the sterilization procedure: Press the controller button “180 °C” for 3 seconds to access the
sterilization program.

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 Figure 29: Initial sterilization display

No sterilization can be started if the CO2 sensor is still plugged-in.

You cannot start the sterilization if the operating mode

HAND is locked. This is displayed by an error message. If
this happens, unlock the operating mode HAND (chap.
7.4).

Figure 30: Error message if operating mode HAND is

locked or if the CO2 sensor is still plugged-in.

• Press “▲”.
The upper display toggles from “OFF” to “ON”.
• Press “MODE” to start the sterilization cycle.
The upper display toggles between the current interior temperature in °C and “StE”.
The indication “ - - - “ in the lower display shows that the CO2 sensor is disconnected.

Figure 31: Alternating display during sterilization

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 CAUTION
The glass door and inner chamber become hot during sterilization.
Danger of burning.
∅ Do NOT touch the glass door and inner surfaces during sterilization.

CAUTION
Interruption of the temperature reaction time.
Ineffective sterilization.
∅ Do NOT open the chamber doors during sterilization.

• The sterilization cycle ends after approx. 12 hours, when the lower display shows “End”.

Figure 32: Alternating display at the end of sterilization

• Turn off the chamber or press “180 °C”, or open the outer door.
• When the inner chamber has cooled down to a value below 60 °C / 140 °F, turn off the chamber and
plug in the CO2 sensor.

The CO2 sensor is temperature resistant up to a maximum temperature of 60 °C / 140 °F

• Turn on the chamber (chap. 5).

The chamber is now ready to operate.

15.5 Aborting the hot-air sterilization

If the sterilization procedure is aborted prematurely, whether effective sterilization has occurred depends
on the time that has elapsed:
• Aborting sterilization after less than 6 hours: Prevents effective sterilization.
• Aborting sterilization after more than 6 hours: The chamber is definitely in the cooling-down phase,
meaning that the necessary duration for the proper sterilization phase has occurred.
Aborting sterilization during the cooling-down phase (after more than 6 hours)
The duration of the entire sterilization is approx. 12 hours. If you want to shorten the sterilization proce-
dure in order to save time, you can abort it during the cooling-down phase, i.e. no sooner than after 6
hours. At this point, the inner temperature is still approx. 140 °C / 284 °F.

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 CAUTION
Glass door and inner chamber become hot during sterilization.
Danger of burning.
∅ Do NOT touch the glass door and inner surfaces for approx. 4 hours after aborting steri-
lization.

Aborting sterilization after less than 6 hours

When aborting the sterilization prematurely, it may be that the cells/pathogens inside the chamber have
not all been killed. You should repeat the sterilization.

CAUTION
Interruption of temperature reaction time.
Ineffective sterilization.
 Repeat the sterilization.

CAUTION
Glass door and inner chamber become hot during sterilization.
Danger of burning.
∅ Do NOT touch the glass door and inner surfaces for approx. 7 hours after aborting steri-
lization.

• When the inner chamber has cooled down to a value below 60 °C / 140 °F, turn off the chamber and
plug in the CO2 sensor.

The CO2 sensor is temperature resistant up to a maximum temperature of 60 °C / 140 °F

• Turn on the chamber (chap. 5).

Three events which can abort the automatic sterilization cycle prematurely:
• A manual abortion (chap. 15.5.1)
• Opening the outer door (chap. 15.5.2)
• Turning off the power to the chamber or a brief power failure (chap. 15.5.3)

15.5.1 Aborting the hot-air sterilization manually

You can abort the sterilization cycle manually.

• Press the controller button “180 °C” for three seconds during the sterilization cycle.
The lower display will show the operating function “StE”, and the value “ON” will appear in the upper
display.
• Press “▲”.
The upper display toggles from “ON” to “OFF“.
• Press “MODE” to end the sterilization cycle.
• Do not open the chamber doors of the until the interior temperature has dropped to 37 °C / 98.6°F.

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• After a manual abortion, the chamber reverts to its standard operational status. The indication “ - - - “
in the lower display shows that the CO2 sensor is disconnected.
As long as the interior temperature remains above the temperature set to trigger the temperature
alarm, the chamber will trigger alarm signals. You can ignore this alarm and turn off the buzzer of the
safety controller by pressing “EXIT”.
• If necessary, repeat the sterilization.
• When the inner chamber has cooled down to a value below 60 °C / 140 °F, turn off the chamber and
plug in the CO2 sensor.

The CO2 sensor is temperature resistant up to a maximum temperature of 60 °C / 140 °F

• Turn on the chamber (chap. 5).

15.5.2 Aborting the hot-air sterilization by opening the outer door

For safety reasons, the sterilization cycle is automatically aborted if you open the outer chamber door.
• Do not open the glass door and close the outer door immediately.
• After an abortion caused by opening the outer door, the chamber reverts to its standard operational
status. The indication “ - - - “ in the lower display shows that the CO2 sensor is disconnected.
As long as the interior temperature remains above the temperature set to trigger the temperature
alarm, the chamber will trigger alarm signals. You can ignore this alarm and turn off the buzzer of the
safety controller by pressing “EXIT”.
• If necessary, repeat the sterilization.
• When the inner chamber has cooled down to a value below 60 °C / 140 °F, turn off the chamber and
plug in the CO2 sensor.

The CO2 sensor is temperature resistant up to a maximum temperature of 60 °C / 140 °F

• Turn on the chamber (chap. 5).

15.5.3 Aborting the hot-air sterilization using the main power switch

For safety reasons, the sterilization cycle is aborted if the chamber is turned off or if there is a power fail-
ure.
Normally you should not terminate sterilization in this way.
• Do not open the chamber doors until the interior temperature has dropped to 37 °C / 98.6 °F.
• After turning on again the chamber, it reverts to its standard operational status. The indication “ - - - “
in the lower display shows that the CO2 sensor is disconnected.
As long as the interior temperature remains above the temperature set to trigger the temperature
alarm, the chamber will trigger alarm signals. You can ignore this alarm and turn off the buzzer of the
safety controller by pressing “EXIT”.
• If necessary, repeat the sterilization.
• When the inner chamber has cooled down to a value below 60 °C / 140 °F, turn off the chamber and
plug in the CO2 sensor.

The CO2 sensor is temperature resistant up to a maximum temperature of 60 °C / 140 °F

• Turn on the chamber (chap. 5).

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16. Maintenance, and service
16.1 Maintenance intervals, service

DANGER
Electrical hazard.
Danger of death.
∅ The chamber must NOT become wet during operation or maintenance work.
∅ Do NOT remove the chamber’s rear panel.
 Before conducting maintenance work, turn off the chamber at the main power switch (2)
and disconnect the power plug
 Ensure all maintenance work is conducted by licensed electricians or experts author-
ized by BINDER.

Ensure regular maintenance work is performed at least once a year.

The warranty becomes void if maintenance work is conducted by non-authorized personnel.

Replace the door gasket only when cold. Otherwise, the door gasket may become damaged.

The CO2 sensor was especially adjusted for the specific chamber. When exchanging the sensor, you
must repeat the CO2 adjustment.
We recommend taking out a maintenance agreement. Please consult BINDER Service.
BINDER telephone hotline: +49 (0) 7462 2005 555
BINDER fax hotline: +49 (0) 7462 2005 93555
BINDER e-mail hotline: [email protected]
BINDER service hotline USA: +1 866 885 9794 or +1 631 224 4340 x3 (toll-free in the USA)
BINDER service hotline Asia Pacific: +852 390 705 04 or +852 390 705 03
BINDER service hotline Russia and CIS +7 495 988 15 16
BINDER Internet website http://www.binder-world.com
BINDER address BINDER GmbH, post office box 102, D-78502 Tuttlingen
International customers, please contact your local BINDER distributor.

16.2 Checking the air jacket heating fan

The user should regularly perform the following checks:
When the chamber is operating, you will be able to observe the air jacket heating fan centered at the top
of the chamber by looking through the ventilation slides at the rear. During operation, the fan must turn
counter-clockwise continuously. For better monitoring, you can turn off the chamber and wait until the fan
has stopped.

16.3 Gas inlet fine filter

When the chamber is operating, the incoming gas passes through a fine gas filter (aseptic filter, filtration
efficiency 99.99%, particle size 0.45 µm). The gas fine filter prevents dirt accumulating in the gas inlet
valves and the tubes leading into the inner chamber, which could be in the gas cylinder or in the supply
tubes.
Service personnel authorized by BINDER will check this filter for pollution at each maintenance interval
and replace it, if appropriate, but at least once a year.

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16.4 Sending the chamber back to BINDER GmbH
If you return a BINDER product to us for repair or any other reason, we will only accept the product upon
presentation of an authorization number (RMA number) that has previously been issued to you. An
authorization number will be issued after receiving your complaint either in writing or by telephone prior
to your sending the BINDER product back to us. The authorization number will be issued following receipt
of the information below:
• BINDER product type and serial number
• Date of purchase
• Name and address of the dealer from which you bought the BINDER product
• Exact description of the defect or fault
• Complete address, contact person and availability of that person
• Exact location of the BINDER product in your facility
• A contamination clearance certificate (chap. 22) must be faxed in advance
The authorization number must be applied to the packaging in such a way that it can be easily recognized
or be recorded clearly in the delivery documents.

For security reasons we cannot accept a chamber delivery if it does not carry an authorization
number.

Return address: BINDER GmbH Gänsäcker 16

Abteilung Service 78502 Tuttlingen
Germany

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17. Disposal
17.1 Disposal of the transport packing

17.1.1 Outer chamber packing

Packing element Material Disposal

Straps to fix packing on pallet

Plastic Plastic recycling
(no image)

Shipping box Cardboard Paper recycling

Edge stuffing, top PE foam Plastic recycling

PE foam Plastic recycling

Pallet with foamed plastic

stuffing

Solid wood
Wood recycling
(IPPC standard)

17.1.2 Packing inside the chamber, equipment

Packing element Material Disposal

Door protection PE foam Plastic recycling
Packing box equipment Cardboard Paper recycling
Insulating air cushion foil PE foil Plastic recycling
Paperboard Cardboard Paper recycling
Do not open. Dispose of with
Silica gel bag Paper with silica gel
normal waste
Cardboard Paper recycling
Sensor packing
PE foam Plastic recycling
Bag for operating manuals PE foil Plastic recycling

If recycling is not possible, all packing parts can also be disposed of with normal waste.

C (E3) 07/2017 page 72/93

17.2 Decommissioning

• Turn off the main power switch (2) and disconnect the chamber from the power supply (pull the power
plug).
• Turn off the CO2 supply. Remove the gas connection.
• Let the inner chamber sufficiently cool down before removing any parts.
• The water pan must not remain filled while the incubator is out of operation. Otherwise condensation
may occur on the inner surfaces, as well as in the injection and suction nozzle of the CO2 sensor com-
partment. If condensation formation has occurred, drops of condensate would leak from the openings
of injection and suction nozzle of the CO2 sensor. In this case, clean and dry the incubator running at
37 °C / 98.6 °F with doors open for at least one hour before loading it with samples. BINDER recom-
mends performing a hot air sterilization of the chamber before commissioning.
• Disassembling the shelf holder takes place inside the chamber. Remove the shelves. Then fold both
lateral parts to the middle and remove them.

You cannot take out the shelf holder of the incubator entirely together with the shelves.

• Temporal decommissioning: See indications for appropriate storage, chap. 3.3.

• Final decommissioning: Dispose of the chamber as described in chap. 17.3 to 17.5.
When restarting the chamber, please pay attention to the corresponding information in chap. 6.2.

17.3 Disposal of the chamber in the Federal Republic of Germany

According to Annex I of Directive 2012/19/EU of the European Parliament and of the Council on waste
electrical and electronic equipment (WEEE), BINDER devices are classified as “monitoring and control
instruments” (category 9) only intended for professional use“. They must not be disposed of at public col-
lecting points.
The chambers bear the symbol for the marking of electrical and electronic equipment man-
ufactured / placed on the market in the EU after 13 August 2005 and be disposed of in sep-
arate collection according to Directive 2012/19/EU on waste electrical and electronic
equipment (WEEE) and German national law for electrical and electronic equipment (El-
ektro- und Elektronikgerätegesetz, ElektroG). WEEE marking: crossed-out wheeled bin with
solid bar under. A significant part of the materials must be recycled in order to protect the
environment.
At the end of the device’s service life, have the chamber disposed of according to the German national
law for electrical and electronic equipment (Elektro- und Elektronikgerätegesetz, ElektroG from 20 Octo-
ber 2015, BGBl. I p. 1739) or contact BINDER service who will organize taking back and disposal of the
chamber according to the German national law for electrical and electronic equipment (Elektro- und El-
ektronikgerätegesetz, ElektroG from 20 October 2015, BGBl. I p. 1739).

CAUTION
Violation against existing law.
∅ Do NOT dispose of BINDER devices at public collecting points.
 Have the device disposed of professionally at a recycling company which is certified
according to the German national law for electrical and electronic equipment (Elektro-
und Elektronikgerätegesetz, ElektroG from 20 October 2015, BGBl. I p. 1739).
or
 Instruct BINDER Service to dispose of the device. The general terms of payment and
delivery of BINDER GmbH apply, which were valid at the time of purchasing the cham-
ber.

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Certified companies disassemble waste (used) BINDER equipment in primary substances for recycling
according to Directive 2012/19/EU. The devices must be free from toxic, infectious or radioactive sub-
stances in order to eliminate any health hazards to the employees of the recycling companies.

Prior to handing the chamber over to a recycling company, it is the user’s responsibility that it
is free from toxic, infectious or radioactive substances.
• Prior to disposal, clean all introduced or residual toxic substances from the chamber.
• Prior to disposal, disinfect the chamber from all sources of infection. Be aware that sources
of infection may also be located outside the inner chamber.
• If you cannot safely remove all toxic substances and sources of infection from the chamber,
dispose of it as “special” waste according to national law.
• Fill out the contamination clearance certificate (chap. 22) and enclose it with the chamber.

WARNING
Contamination of the device with toxic, infectious or radioactive substances.
Danger of intoxication.
Danger of infection.
∅ NEVER take a chamber contaminated with toxic substances or sources of infection for
recycling according to Directive 2012/19/EU.
 Prior to disposal, remove all toxic substances and sources of infection from the cham-
ber.
 A chamber from which all toxic substances or sources of infection cannot be safely
removed must be considered as “special” waste according to national law. Dispose of it
accordingly.

17.4 Disposal of the chamber in the member states of the EU except for the Fed-
eral Republic of Germany
According to Annex I of Directive 2012/19/EU of the European Parliament and of the Council on waste
electrical and electronic equipment (WEEE), BINDER devices are classified as “monitoring and control
instruments” (category 9) only intended for professional use“. They must not be disposed of at public col-
lecting points.
The chambers bear the symbol for the marking of electrical and electronic equipment man-
ufactured / placed on the market in the EC after 13 August 2005 and be disposed of in
separate collection according to the Directive 2012/19/EU on waste electrical and electron-
ic equipment (WEEE). WEEE marking: crossed-out wheeled bin with solid bar under.
At the end of the device’s service life, notify the distributor who sold you the device, who
will take back and dispose of the chamber according to the Directive 2012/19/EU on waste
electrical and electronic equipment (WEEE).

CAUTION
Violation against existing law.
∅ Do NOT dispose of BINDER devices at public collecting points.
 Have the device disposed of professionally at a recycling company that is certified ac-
cording to conversion of the Directive 2012/19/EU into national law.
or
 Instruct the distributor who sold you the device to dispose of it. The agreements apply
that were agreed with the distributor when purchasing the chamber (e.g. his general
terms of payment and delivery).
 If your distributor is not able to take back and dispose of the chamber, please contact
BINDER service.

C (E3) 07/2017 page 74/93

Certified companies disassemble waste (used) BINDER equipment in primary substances for recycling
according to Directive 2012/19/EU. The devices must be free from toxic, infectious or radioactive sub-
stances in order to eliminate any health hazards to the employees of the recycling companies.

Prior to handing the chamber over to a recycling company, it is the user’s responsibility that it
is free from toxic, infectious or radioactive substances.
• Prior to disposal, clean all introduced or residual toxic substances from the chamber.
• Prior to disposal, disinfect the chamber from all sources of infection. Be aware that sources
of infection may also be located outside the inner chamber.
• If you cannot safely remove all sources of infection and toxic substances from the chamber,
dispose of it as “special” waste according to national law.
• Fill out the contamination clearance certificate (chap. 22) and enclose it with the chamber.

WARNING
Contamination of the device with toxic, infectious or radioactive substances.
Danger of intoxication.
Danger of infection.
∅ NEVER take a chamber contaminated with toxic substances or sources of infection for
recycling according to Directive 2012/19/EU.
 Prior to disposal, remove all toxic substances and sources of infection from the cham-
ber.
 A chamber from which all toxic substances or sources of infection cannot be safely
removed must be considered as “special” waste according to national law. Dispose of it
accordingly.

17.5 Disposal of the chamber in non-member states of the EU

CAUTION
Alteration of the environment.
 For final decommissioning and disposal of the CO2 incubator, please contact BINDER
Service.
 Follow the statutory regulations for appropriate, environmentally friendly disposal.

The main board of the CO2 incubator includes a lithium cell. Please dispose of it according to national
regulations.

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18. Troubleshooting
Fault description Possible cause Required measures
General
Check connection to power sup-
No power supply.
ply.
Check power supply for voltage of
Wrong voltage.
120 V / 230 V.
Nominal temperature exceeded
by 10 °C due to chamber failure.
Chamber without function. Over temperature protective de- Contact BINDER service.
vice (class 1) responds (chap.
9.1).
The miniature fuse for overcur- Check chamber fuse and replace it
rent protection has triggered if appropriate. If it responds again,
(chap. 19.2). contact BINDER service.
Controller defective. Contact BINDER service.
The outer chamber door is not
Message “dor” is displayed. Close chamber door properly.
closed properly.
Heating
Pt 100 sensor defective.
Chamber does not heat up. Heating element defective. Contact BINDER service.
Semiconductor relay defective.
Doors not closed. Close door properly.
Door gaskets defective. Replace door gaskets.
Temperature inside too low. Controller defective.
Contact BINDER service.
Pt 100 sensor defective.
Controller not adjusted. Calibrate and adjust controller.
Doors not closed. Close door properly.
Temperature inside too low. Door gaskets defective. Replace door gaskets.
Message “tol” is displayed Check and, if appropriate, set the
(temperature tolerance range operating function “AL.12” (tem-
alarm) Wrong controller setting.
perature alarm threshold) (chap.
7.3). Value should be ≥ 1 K.
Select cooler place of installation
Installation site too warm.
(chap. 3.4).
Difference between the set tem- Difference between the set tem-
perature and the ambient tem- perature and the ambient tempera-
Temperature inside too high. perature too low. ture at least 7°.
Too much external heat load. Reduce heat load.
Controller defective.
Contact BINDER service.
Semiconductor relay defective.
Controller not adjusted. Calibrate and adjust controller
Select cooler place of installation
Installation site too warm.
(chap. 3.4).
Difference between the set tem- Difference between the set tem-
perature and the ambient tem- perature and the ambient tempera-
Temperature inside too high. perature too low. ture at least 7°.
Message “tol” is displayed (tem- Too much external heat load. Reduce heat load.
perature tolerance range alarm). Semiconductor relay defective. Contact BINDER service.
Check and, if appropriate, set the
operating function “AL.12” (tem-
Wrong controller setting.
perature alarm threshold) (chap.
7.3). Value should be ≥ 1 K.

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Fault description Possible cause Required measures
Heating (continued)
Temperature inside too low. Limit temperature reached. Safe- Check the setting of the safety
Message “otc” is displayed ty controller (chap. 9.2) set too controller. Choose a suitable limit
(safety controller alarm). low. value (chap. 9.2).
Temperature inside too high. Controller defective.
Message “otc” is displayed Safety controller (chap. 9.2) de- Contact BINDER service.
(safety controller alarm). fective.
Chamber heating permanently, Semiconductor relay defective.
Contact BINDER service.
set point not held. Controller defective.
Message “End” is displayed Sterilization cycle completed. Start up the chamber (chap. 5).
Gas
CO2 cylinder is not connected
Correctly connect the gas cylinder.
correctly.
Connected gas cylinder is closed
Pressure alarm. Message “P.Lo” or empty. Open or replace gas cylinder.
is displayed (low pressure
alarm). Check the tube system for dirt
Gas hose is dirty or obstructed. accumulation or obstruction, clean
or replace it.
Pressure sensor system defective Contact BINDER service.
Doors not closed. Close door properly.
Door gaskets defective. Replace door gaskets.
CO2 cylinder is not connected
Correctly connect the gas cylinder.
correctly.
Connected gas cylinder is closed
CO2 concentration inside too low Open or replace gas cylinder.
or empty.
(controller display).
Check the tube system for dirt
Gas hose is dirty or obstructed. accumulation or obstruction, clean
or replace it.
CO2 sensor defective. Contact BINDER service.
Controller not adjusted. Calibrate and adjust controller
Doors not closed. Close door properly.
CO2 concentration inside too low Door gaskets defective. Replace door gaskets.
(controller display). Message Check and, if appropriate, set the
“con” is displayed (CO2 toler- operating function “AL.2” (CO2
ance range alarm) Wrong controller setting.
alarm threshold) (chap. 7.3). Value
should be ≥ 1.0 vol.-%.CO2.
CO2 sensor defective.
CO2 concentration inside too Contact BINDER service.
Controller defective
high (controller display).
Controller not adjusted. Calibrate and adjust controller
CO2 concentration inside too Check and, if appropriate, set the
high (controller display). Mes- operating function “AL.2” (CO2
Wrong controller setting.
sage “con” is displayed (CO2 alarm threshold) (chap. 7.3). Value
tolerance range alarm) should be ≥ 1.0 vol.-%.CO2.
Actual value of CO2 deviates
largely compared with a refer- Transfer the cultures to another
ence method. CO2 sensor system defective. incubator and contact BINDER
The pH indicator of the cell me- Service.
dium changes its normal color
Recovery time (up to 5 vol.-%
CO2) after doors were open for 2 CO2 sensor system defective. Contact BINDER service.
minutes is < 2 minutes.
Recovery time (up to 5 vol.-% Obstructed gas supply. Check gas supply (cylinder, con-
CO2) after doors were open for 2 Insufficient CO2 input pressure. nections, hose system).
minutes is > 10 minutes. Gas fine filter obstructed. Contact BINDER service.

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Fault description Possible cause Required measures
Heating (continued)
Door gaskets defective. Replace door gaskets.
Unusually high gas consump- Gas sensor defective. Contact BINDER service.
tion. Gas fine filter not connected cor-
Contact BINDER service.
rectly
Humidity
Fill the water pan with water up to
the marking on the inner pan with
distilled, sterile water. The pan
No or too low humidity inside. Water pan empty.
must have thorough contact to the
bottom of the inner chamber
(chap. 4.3).
Water pan filled with water when Empty water pan when incubator
Condensations inside the cham- incubator is not operating is not operating.
ber. Doors not closed. Close door properly.
Door gaskets defective. Replace door gaskets.
Place the chamber on a BINDER
Chamber placed on very cold
stand to increase the distance to
floor.
the floor.
Condensation on the door. Doors not closed. Close door properly.
Door gaskets defective. Replace door gaskets.
Door heating defective Contact BINDER service.
Controller
No chamber function. Dark dis-
Main power switch is off. Turn on the main power switch.
play.
No entries to controller keypad Unlock keyboard locking (chap.
Keyboard locking activated.
possible. 7.4).
No access to menu “USER”. User code incorrect. Contact BINDER service.
Cause of disturbance not re-
moved correctly
Remove cause of disturbance. If
The EXIT button permits resetting
EXIT button does not cancel the the EXIT button still does not can-
alarm messages for temperature
alarm indication. cel the indication, contact BINDER
and CO2 only with in a tolerance
service.
sector of +/- 1 °C resp. +/- 1 vol.-
%
CO2 concentration shown as
Failure of CO2 sensor. Contact BINDER service.
“-0.4”
Alarm message “997” is dis- Failure of temperature sensor for
Contact BINDER service.
played door heating
Alarm message “998” is dis- Failure of temperature sensor for
Contact BINDER service.
played safety controller
Alarm message “999” is dis- Failure of temperature sensor for
Contact BINDER service.
played interior heating
Alarm message “ - - - ” is dis-
CO2 sensor not connected Connect the CO2 sensor.
played
Sterilization program started with Enter the password for unlocking
operating mode HAND locked and then start again the steriliza-
Alarm message “Loc” (LOCK). tion.
Sterilization program started with Pull the CO2 sensor and remove it
the CO2 sensor still plugged-in. from the incubator.

Only qualified service personnel authorized by BINDER must perform repair.

Repaired chambers must comply with the BINDER quality standards.

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19. Technical description
19.1 Factory calibration and adjustment
This chamber was calibrated and adjusted in the factory. Calibration and adjustment were performed
using standardized test instructions, according to the QM DIN EN ISO 9001 system applied by BINDER
(certified since December 1996 by TÜV CERT). All test equipment used is subject to the administration of
measurement and test equipment that is also a constituent part of the BINDER QM DIN EN ISO 9001
systems. They are controlled and calibrated to a DKD-Standard at regular intervals.
A record of this calibration and adjustment is part of the BINDER test certificate of the chamber.
Adjustment in factory:
• Temperature: 37 °C / 98.6 °F measured in the center of the usable volume
• CO2: 0 vol.-% CO2 (100 vol.-% N2) and 5 vol.-% CO2 (sensor head directly exposed to analyzed test
gas)
Suitable reference methods applicable for the user for comparison between reference measuring results
and the display readings of the controller(s) are explained in chap. 12.
During factory calibration and adjustment, an electronic temperature measuring and display device is
used, which is traceable to an acknowledged standards/calibration institution (DKD or PTB for Germany),
bearing a valid calibration certificate.
During factory calibration and adjustment, test gases with an analyzed concentration and with adapted
flow quantity serve to calibrate the sensor system for CO2. The sensor head is exposed directly to the test
gas.

19.2 Over current protection

A miniature fuse accessible from the outside protects the device against over current. The miniature fuse
is located at the rear of the chamber below the strain relief of the power cord. The fuse holder is equipped
with a fuse clip 5mm x 20 mm. Replace the fuse only with a substitute of the same ratings. Refer to the
technical data of the respective device type. If the fuse is blown, please inform an electronic engineer or
BINDER service.

19.3 Definition of usable volume

The usable volume illustrated below is calculated as follows:
A, B, C = internal dimensions (W, H, D)
c a, b, c = wall separation
C
a = 0.1*A
b = 0.1*B
b B
c = 0.1*C
a a
VUSE = (A - 2 * a) * (B - 2 * b) * (C - 2 * c)

c b
Figure 33: Determination of the useable volume
A

The technical data refers to the so defined usable volume.

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 Do NOT place samples outside this usable volume.
Do NOT load this volume by more than half to enable sufficient airflow inside the CO2 incuba-
tor.
Do NOT divide the usable volume into separate parts with large area samples.
Do NOT place samples too close to each other in order to permit circulation between them
and thus obtain a homogenous distribution of temperature and CO2.

19.4 C 170 technical data

Exterior dimensions
Width, net mm / inch 740 / 29.13
Height including feet mm / inch 870 / 34.25
Depth, net mm / inch 715 / 28.15
Depth including door handle, I-triangle, and
mm / inch 795 / 31.30
connections
Wall clearance rear (minimum) mm / inch 100 / 3.94
Wall clearance side (minimum) mm / inch 50 / 1.97
Doors
Number of doors 1
Number of inner glass doors 1
Interior dimensions
Width mm / inch 560 / 22.05
Height mm / inch 600 / 23.62
Depth mm / inch 500 / 19.69
Interior volume l / cu.ft. 168 / 5.93
Shelves
Number of shelves, series 3
Number of shelves, max. 8
mm x mm 538 x 447
Size of shelf, width x depth
inch x inch 21.18 x 17.60
Weight
Weight (empty) kg / lbs 110 / 242.5
Temperature data
Temperature range, 7 °C / 12.6 °F above ambient,
°C / °F 50 / 122
up to
Temperature fluctuation ≤ +/- K 0.1
Temperature uniformity (variation) at 37 °C/ 98.6 °F +/- K 0.3
Recovery time after door open for 30 sec
minutes 6
at 37 °C / 98.6°F
Humidity data
Humidity range % r.H. 90 to 95
CO2 data
CO2 range vol.-% CO2 0 to 20
Setting accuracy vol.-% CO2 0.1
Recovery time after door open for 30 sec
minutes 7
at 5 vol.-% CO2
Drift-free CO2 infrared absorption measurement
CO2 measurement
system
Connection hose nozzle DN6 for CO2
Connection to the chamber for hose with internal di- mm / inch 6 / 0.24
ameter

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Electrical data
(model versions C170-230V-R, C170-230V-L)
IP system of protection acc. to EN 60529 IP 20
Nominal voltage at 50 Hz power frequency V 230
(+/-10%) at 60 Hz power frequency V 230
Current type 1N~
Nominal power kW 1.10
Power plug (IEC connector plug) Shock-proof plug
Installation category acc. to IEC 61010-1 II
Pollution degree acc. to IEC 61010-1 2
Chamber fuse 5x20mm / semi time-lag / 10 A
Different electrical data for C 170-UL constructed for the USA and Canada
(model versions C170UL-120V-R, C170UL-120V-L)
Nominal voltage (±10 %) at 60 Hz power frequency V 120
Power plug (IEC connector plug) NEMA 5-20P
Chamber fuse 6.3 X 32 mm / 250V / super-time-lag TT / 16A
Environment-specific data
Energy consumption at 37 °C / 98.6°F Wh/h 90
The recovery times of the gas concentrations inside the chamber following the door being opened coin-
cide with a connection pressure of 2.0 bar / 29 psi. Decreasing supply pressure results in longer recovery
times.
All technical data is specified for unloaded chambers with standard equipment at an ambient temperature
of +22 °C +/- 3 °C / 71.6 °F +/- 5.4 °F and a power supply voltage fluctuation of +/-10. The recovery times
are determined in accordance to BINDER Factory Standard Part 1:2015 following DIN 12880:2007. The
other technical data is determined in accordance to BINDER Factory Standard Part 2:2015 and DIN
12880.
All indications are average values, typical for chambers produced in series. We reserve the right
to change technical specifications at any time.

19.5 Important conversion data for non-SI units

1 ft = 0.305 m = 0.000305 km
1 m = 100 cm = 3.28 ft = 39.37 inch
1 km = 1000 m = 3280.83 ft
1 mbar = 0.0145 psi

19.6 Conversion table for gas inlet pressures, bar – psi

bar psi bar psi bar psi

1 14.5 3 43.5 5 72.5
1.5 21.7 3.5 50.7 5.5 79.7
2 29.0 4 58.0 6 87.0
2.5 36.3 4.5 65.2

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19.7 Equipment and options (extract)

To operate the chamber, use only original BINDER accessories or accessories / components
from third-party suppliers authorized by BINDER. The user is responsible for any risk arising
from using unauthorized accessories.

Regular equipment
Microprocessor display controller with 2-channel technology for temperature and CO2
CO2 infra-red absorption measuring system
Fan-assisted air jacket system
Hot-air sterilization at 180 °C / 356°F
Gas mixing head
Weldless deep-drawn inner chamber made of stainless steel V2A (German material no. 1.4301,
US equivalent AISI 304), polished
Electronic error auto-diagnosis system with zero-voltage relay output
Temperature safety device class 3.1 acc. to DIN 12880:2007
Tightly closing inner glass door
3 perforated shelves, stainless steel (German material no. 1.4016, US equivalent AISI 430)
C 170-UL: cUL test mark

Options / accessories
Perforated shelf, stainless steel
Door hinged left (option only available when ordering the chamber, no retrofitting)
Silicone access ports, closable with 2 silicone plugs 30 mm / 1.18 in , rear, left or right side
Analog outputs 4-20mA for temperature and CO2, with DIN socket 6-poles, DIN plug included
Base on castors
Stacking adapter for direct, thermally decoupled stacking
Gas cylinder connection kit
Pressure reducer
Cleaning kit (neutral cleaning agent, disinfection spray and lint-free disposable wipes, protective gloves
and safety goggles)
Calibration of temperature and CO2 including certificate
Spatial temperature measurement including certificate
Spatial temperature measurement acc. to DIN 12880:2007 including certificate
Qualification folder

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19.8 Accessories and spare parts (extract)

BINDER GmbH is responsible for the safety features of the chamber only, provided skilled
electricians or qualified personnel authorized by BINDER perform all maintenance and repair,
and if components relating to chamber safety are replaced in the event of failure with original
spare parts. The user is responsible for any risks arising from using unauthorized accesso-
ries/components.

Description Art. no.

Perforated shelf, stainless steel 6004-0142
Base on castors 9051-0029
Stacking adapter for direct, thermally decoupled stacking 9051-0036
Gas cylinder connection kit for CO2 8012-0014
Pressure reducer 6013-0016
Calibration certificate for temperature and CO2 8012-0228
Cleaning kit (neutral cleaning agent, disinfection spray and lint-free disposable
8012-0503
wipes), protective gloves and safety goggles
Neutral cleaning agent, 1 kg 1002-0016
Shelf holder, stainless steel 6004-0145
Door gasket chamber door 6005-0243
Door gasket glass door 6005-0508
Water pan 4022-0325
Chamber fuse 5x20mm 250V 10A semi time-lag (M) (C 170 230V) 5006-0012
Chamber fuse 6.3 x 32 mm 250V 16A time-lag (T) (C 170-UL) 5006-0033
CO2 sensor 5002-0066
Filter cap for CO2 sensor 6014-0033
Gas fine filter 8009-0369
Power cable with IEC connector plug for EU 5023-0222
Power cable with IEC connector plug for Switzerland 8012-0218
Power cable with IEC connector plug for England 8012-0220
Power cable with IEC connector plug for USA 5023-0220

Validation service Art. no.

Qualification folder IQ-OQ 8012-0875
Qualification folder IQ-OQ-PQ 8012-0962
Execution of IQ-OQ DL410200
Execution of IQ-OQ-PQ DL440500

Calibration service Art. no.

Calibration of CO2 including certificate DL300401
Calibration of temperature including certificate (1 measuring point) DL300101
Spatial temperature measurement including certificate (9 measuring points) DL300109
Spatial temperature measurement including certificate (18 measuring points) DL300118
Spatial temperature measurement including certificate (27 measuring points) DL300127

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19.9 Dimensions C 170

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20. EU Declaration of Conformity

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C (E3) 07/2017 page 86/93
21. Product registration

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22. Contamination clearance certificate
Unbedenklichkeitsbescheinigung
22.1 For chambers located outside the USA and Canada
Declaration regarding safety and health
Erklärung zur Sicherheit und gesundheitlichen Unbedenklichkeit
The German Ordinance on Hazardous Substances (GefStofV), and the regulations regarding safety at
the workplace require that this form be filled out for all products that are returned to us, so that the safety
and the health of our employees can be guaranteed.
Die Sicherheit und Gesundheit unserer Mitarbeiter, die Gefahrstoffverordnung GefStofV und die Vorschriften zur
Sicherheit am Arbeitsplatz machen es erforderlich, dass dieses Formblatt für alle Produkte, die an uns zurückges-
chickt wird.

Note: A repair is not possible without a completely filled out form.

Ohne Vorliegen des vollständig ausgefüllten Formblattes ist eine Reparatur nicht möglich.

• A completely filled out form must be transmitted via Fax (+49 (0) 7462 2005 93555) or by letter in ad-
vance, so that this information is available before the equipment/component part arrives. A second
copy of this form must accompany the equipment/component part. In addition, the carrier should be
notified.
Eine vollständig ausgefüllte Kopie dieses Formblattes soll per Telefax (Nr. +49 (0) 7462 2005 93555) oder Brief
vorab an uns gesandt werden, so dass die Information vorliegt, bevor das Gerät/Bauteil eintrifft. Eine weitere
Kopie soll dem Gerät/Bauteil beigefügt sein. Ggf. ist auch die Spedition zu informieren.
• Incomplete information or non-conformity with this procedure will inevitably lead to substantial delays
in processing. Please understand the reason for this measure, which lies outside our area of influence
and will help us to speed up this procedure.
Unvollständige Angaben oder Nichteinhalten dieses Ablaufs führen zwangsläufig zu beträchtlichen Verzöger-
ungen in der Abwicklung. Bitte haben Sie Verständnis für Maßnahmen, die außerhalb unserer Einflussmöglich-
keiten liegen und helfen Sie mit, den Ablauf beschleunigen.

• Please print and fill out this form completely.

Bitte unbedingt vollständig ausfüllen!

1. Unit/ component part / type: / Gerät / Bauteil / Typ:

2. Serial No./ Serien-Nr.:

3. Details about utilized substances / biological substances / Einzelheiten über die eingesetzten
Substanzen/biologische Materialien:

3.1 Designations / Bezeichnungen:

a) ____________________________________________________________________________

b) ____________________________________________________________________________

c) ____________________________________________________________________________

3.2 Safety measures required for handling these substances / Vorsichtsmaßnahmen beim Um-
gang mit diesen Stoffen:
a) ____________________________________________________________________________

b) ____________________________________________________________________________

c) ____________________________________________________________________________

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3.3 Measures to be taken in case of skin contact or release into the atmosphere / Maßnahmen
bei Personenkontakt oder Freisetzung:
a) ____________________________________________________________________________

b) ____________________________________________________________________________

c) ____________________________________________________________________________

d) ____________________________________________________________________________

3.4 Other important information that must be taken into account / Weitere zu beachtende und
wichtige Informationen:
a) ____________________________________________________________________________

b) ____________________________________________________________________________

c) ____________________________________________________________________________

4. Declaration on the risk of these substances (please checkmark the applicable items) /
Erklärung zur Gefährlichkeit der Stoffe (bitte Zutreffendes ankreuzen) :

 4.1 For non toxic, non radioactive, biologically harmless materials / für nicht giftige, nicht radi-
oaktive, biologisch ungefährliche Stoffe:
We hereby guarantee that the above-mentioned unit / component part… / Wir versichern, dass o.g.
Gerät/Bauteil...
 Has not been exposed to or contains any toxic or otherwise hazardous substances / weder giftige noch
sonstige gefährliche Stoffe enthält oder solche anhaften.
 That eventually generated reaction products are non-toxic and also do not represent a hazard / auch
evtl. entstandene Reaktionsprodukte weder giftig sind noch sonst eine Gefährdung darstellen.
 Eventual residues of hazardous substances have been removed / evtl. Rückstände von Gefahrstoffen
entfernt wurden.

 4.2 For toxic, radioactive, biologically harmful or hazardous substances, or any other hazard-
ous materials / für giftige, radioaktive, biologisch bedenkliche bzw. gefährliche Stoffe oder
anderweitig gefährliche Stoffe.
We hereby guarantee that … / Wir versichern, dass …
 The hazardous substances, which have come into contact with the above-mentioned equip-
ment/component part, have been completely listed under item 3.1 and that all information in this re-
gard is complete / die gefährlichen Stoffe, die mit dem o.g. Gerät/Bauteil in Kontakt kamen, in 3.1 aufgelistet
sind und alle Angaben vollständig sind.
 That the unit /component part has not been in contact with radioactivity / das Gerät/Bauteil nicht mit Ra-
dioaktivität in Berührung kam

5. Kind of transport / transporter / Transportweg/Spediteur:

Transport by (means and name of transport company, etc.) Versendung durch (Name Spediteur o.ä.)

__________________________________________________________________________________

Date of dispatch to BINDER GmbH / Tag der Absendung an BINDER GmbH:

___________________________________________________________________________________

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We hereby declare that the following measures have been taken / Wir erklären, dass folgende
Maßnahmen getroffen wurden:
 Hazardous substances were removed from the unit including component parts, so that no hazard ex-
ists for any person in the handling or repair of these items / das Gerät/Bauteil wurde von Gefahrstoffen be-
freit, so dass bei Handhabung/Reparaturen für die betreffenden Person keinerlei Gefährdung besteht
 The unit was securely packaged and properly identified / das Gerät wurde sicher verpackt und vollständig
gekennzeichnet.
 Information about the hazardousness of the shipment (if required) has been provided to the trans-
porter / der Spediteur wurde (falls vorgeschrieben) über die Gefährlichkeit der Sendung informiert.
We hereby commit ourselves and guarantee that we will indemnify BINDER GmbH for all damages that
are a consequence of incomplete or incorrect information provided by us, and that we will exempt
BINDER GmbH from eventual damage claims by third parties./ Wir versichern, dass wir gegenüber BINDER
für jeden Schaden, der durch unvollständige und unrichtige Angaben entsteht, haften und BINDER gegen eventuell
entstehende Schadenansprüche Dritter freistellen.
We are aware that, in accordance with Article 823 of the German Civil Code (BGB), we are directly liable
with regard to third parties, in this instance especially the employees of BINDER GmbH, who have been
entrusted with the handling / repair of the unit / component. / Es ist uns bekannt, dass wir gegenüber Dritten –
hier insbesondere mit der Handhabung/Reparatur des Geräts/des Bauteils betraute Mitarbeiter der Firma BINDER -
gemäß §823 BGB direkt haften

Name: _______________________________________________________________________

Position/Title: _______________________________________________________________________

Date / Datum: _______________________________________________________________________

Signature / Unterschrift: _______________________________________________________________

Company stamp / Firmenstempel:

Equipment that is returned to the factory for repair must be accompanied by a completely filled
out contamination clearance certificate. For service and maintenance on site, you must submit
such a contamination clearance certificate to the service technician before the start of any
work. No repair or maintenance of the equipment is possible, without a properly filled out con-
tamination clearance certificate.

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22.2 For chambers in the USA and Canada

Product Return Authorization Request

Please complete this form and the Customer Decontamination Declaration (next 2 pages) and attach the
required pictures. E-mail to: [email protected]
After we have received and reviewed the complete information we will decide on the issue of a RMA
number. Please be aware that size specifications, voltage specifications as well as performance specifi-
cations are available on the internet at www.binder-world.us at any time.
Take notice of shipping laws and regulations.

Please fill:
Reason for return request  Duplicate order
 Duplicate shipment
 Demo Page one completed by sales
 Power Plug / Voltage 115V / 230 V / 208 V / 240V
 Size does not fit space
 Transport Damage Shock watch tripped? (pictures)
 Other (specify below)
_____________________
Is there a replacement PO?  Yes  No
If yes -> PO #
If yes -> Date PO placed

Purchase order number

BINDER model number
BINDER serial number
Date unit was received

Was the unit unboxed?  Yes  No

Was the unit plugged in?  Yes  No
Was the unit in operation?  Yes  No

Pictures of unit attached?  Yes  No Pictures have to be attached!

Pictures of Packaging at-  Yes  No
tached?

Customer Contact Information Distributor Contact Information

Name
Company
Address
Phone
E-mail

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Customer (End User) Decontamination Declaration
Health and Hazard Safety declaration

To protect the health of our employees and the safety at the workplace, we require that this form is com-
pleted by the user for all products and parts that are returned to us. (Distributors or Service Organizations
cannot sign this form)

NO RMA number will be issued without a completed form. Products or parts returned to our
NY warehouse without a RMA number will be refused at the dock.

A second copy of the completed form must be attached to the outside of the shipping box.
1. Unit/ component part / type:

2. Serial No.

3. List any exposure to hazardous liquids, gasses or substances and radioactive material
3.1 List with MSDS sheets attached where available or needed
(if there is not enough space available below, please attach a page):

a) ____________________________________________________________________________

b) ____________________________________________________________________________

c) ____________________________________________________________________________

3.2 Safety measures required for handling the list under 3.1

a) ____________________________________________________________________________

b) ____________________________________________________________________________

c) ____________________________________________________________________________
3.3 Measures to be taken in case of skin contact or release into the atmosphere:

a) ____________________________________________________________________________

b) ____________________________________________________________________________

c) ____________________________________________________________________________

d) ____________________________________________________________________________

3.4 Other important information that must be considered:

a) ____________________________________________________________________________

b) ____________________________________________________________________________

c) ____________________________________________________________________________

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4. Declaration of Decontamination
For toxic, radioactive, biologically and chemically harmful or hazardous substances, or any other
hazardous materials.
We hereby guarantee that
4.1 Any hazardous substances, which have come into contact with the above-mentioned equipment /
component part, have been completely listed under item 3.1 and that all information in this regard is
complete.
4.2 That the unit /component part has not been in contact with radioactivity
4.3 Any Hazardous substances were removed from the unit / component part, so that no hazard exists
for a persons in the shipping, handling or repair of these returned unit
4.4 The unit was securely packaged in the original undamaged packaging and properly identified on the
outside of the packaging material with the unit designation, the RMA number and a copy of this dec-
laration.
4.5 Shipping laws and regulations have not been violated.
I hereby commit and guarantee that we will indemnify BINDER Inc for all damages that are a con-
sequence of incomplete or incorrect information provided by us, and that we will indemnify and
hold harmless BINDER Inc. from eventual damage claims by third parties..

Name: _______________________________________________________________________

Position: _______________________________________________________________________

Company: _______________________________________________________________________

Address: _______________________________________________________________________

Phone #: _______________________________________________________________________

Email: _______________________________________________________________________

Date: _______________________________________________________________________

Signature: _______________________________________________________________________

Equipment returned to the NY warehouse for repair must be accompanied by a completed

customer decontamination declaration. For service and maintenance works on site, such a
customer decontamination declaration must be submitted to the service technician before the
start of work. No repair or maintenance of the equipment is possible without a completed form.

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