Curriculum Vitae

Nastassia M. Navasiolava

Nationality: Belarusian
Date and place of birth: December 7, 1975, Minsk, Belarus
Cell: 33 (0) 6 71 16 85 30
e-mail: [email protected]
webpage: https://sites.google.com/site/navasiolava

Objective
Progress in biomedical science field

Current situation

Postdoctoral Research Fellow in the team “Vascular functions and environmental

conditions”, Integrated Mitochondrial and Neuro-Vascular Biology Lab, Angers Faculty of
Medicine, France; member of CaDyWEC international lab (Cardiovascular Dysfunction-
Weightlessness-Environmental Conditions)

Education & appointments

1991-1996: Medical Student, Minsk State Medical Institute, Minsk, Belarus

1996: MD in Stomatology
1999-2007: Technician to Assistant Scientist, Institute of Physiology, National Academy of
Sciences, Minsk, Belarus
2007-2010: PhD Student in joint tuition between Angers University, France, and Institute of
Physiology, Minsk, Belarus
2010 November 2: PhD in Physiology “The mechanisms of vascular dysfunction induced by
simulated microgravity”
2011 November 14-25: Training «Animal experimentation agreement, level I», Marseille,
France
2010- : Postdoc “Cardiovascular impairment associated with physical inactivity and
metabolic disorders”, CNRS UMR 6214 - INSERM U1083, Angers, France

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 Experience & Research projects

IN ANIMAL

Currently: CNRS UMR 6214 - INSERM U771, Angers Faculty of Medicine, France
The effects of traditional Chinese herbs on diabetes-induced cardiovascular dysfunction in
rat
July-August 2009: Institute of Physiology, Minsk, Belarus
The role of circulating factors in vascular dysfunction induced by physical inactivity and
microgravity in rat models of tail suspension and antiorthostatic hypokinesia
July-August 2008: Institute of Physiology, Minsk, Belarus
Endotoxin translocation in rat models of microgravity and environmental constraints
September 2007 – June 2008: CNRS UMR 6214 - INSERM U771, Angers Faculty of Medicine,
France
Influence of environmental conditions (14-day social isolation, constraint and hindlimb
unloading) to skin microcirculatory functions and baroreflex sensitivity in rat
July-August 2007: Institute of Physiology, Minsk, Belarus
Metabolic intensity, proinflammatory cytokines and thermoregulation in rat during
antiorthostatic and horizontal hypokinesia
January-February 2007: Institute of Physiology, Minsk, Belarus
Blood pressure and visceral nociception in rat in response to 7 days of antiorthostatic
hypokinesia or vertical rocking

IN HUMAN

Currently: CNRS UMR 6214 - INSERM U771, Angers Faculty of Medicine, France
Analysis of the results for the protocol «Effects of 2-months head-down bed rest in healthy
volunteers»:
Cardiovascular deconditioning at micro- and macrovascular level
Prophylactic effect of Chinese herbs
Prophylactic effect of exercise+vibration countermeasure
July-August 2010: Institute of Biomedical Problems, Moscow, Russia
The effects of 5-day dry immersion in healthy men:
Cardiovascular deconditioning
Body fluid compartments
Metabolic impairment
December 2006 and May-June 2007: Institute of Biomedical Problems, Moscow, Russia
The effects of 7-day dry immersion in healthy men:
State of endothelium and microcirculatory functions
Cardiovascular responses to stand-test
Water and sodium metabolism and its hormonal regulation
September 2006: Vascular Investigation Department of Angers University Hospital, France
Reproducibility of iontophoretic responses to acetylcholine chloride and sodium
nitroprusside at skin microcirculatory level in healthy volunteers

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 Specific professional skills

Research managing:
Bibliographic research
Design of protocol
Application for funding
Data statistical treatment and analysis
Intermediate report writing
Manuscript writing

Practical skills in small rodents:

Standard laboratory techniques (handling; subcutaneous, intraperitoneal,
intravenous and intramuscular injections; blood collection; inhalational anesthesia;
euthanasia)
Blood pressure measurement (intravascular at femoral level; indirect with tail cuff
method)
Indirect calorimetry (with MM-100 metabolic monitor system)
Isolated vessels (wire-myograph technique)
Telemetry (DSI system)
Thermocouple thermometry
Laser-Doppler flowmetry coupled with iontophoresis
Tail flick latency
Visceral nociception latency
Hindlimb unloading model
Antiorthostatic and horizontal hypokinesia models with restraining boxes
Isolated perfused kidney model

Practical skills in human:

Dry immersion model
Study of skin microcirculatory functions (laser-Doppler flowmetry coupled with
iontophoresis of vasoactive substances)
Performance of orthostatic tests with continuous recording of finger blood pressure
wave (Finapres) and standard ECG
Bio-impedance measurements (Xitron multifrequency impedance device)
Direct measurements of glucose concentration (Accu Check Performa glucometer),
hemoglobin (HemoCue hemoglobin analyzer), hematocrit (microhematocrit method)
Preparation of blood and urine samples according to further analysis

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 Teaching

April 5, 2012 & March 3, 2011: Angers Faculty of Medicine, France

Delivery of 2-hours course “Effets cardio-vasculaires de la microgravité et de l’inactivité
physique” (“Cardiovascular effects of microgravity and physical inactivity”) for Master 1
medical students

November 29, 2010: Angers Faculty of Medicine, France

Delivery of 2-hours course “Inactivité physique et dysfonction vasculaire” (“Physical
inactivity and vascular dysfunction”) for Master 2 medical students

Supervision

Currently: Angers Faculty of Medicine, France

Master 2 “Physiopathology”, 6-months research project “The effects of traditional Chinese
herbs on diabetes-induced cardiovascular deconditioning in rat” (Asmaa Alameddine)

March-April 2011: Angers Faculty of Medicine, France

Master 1 research project “The particularities of Goto-Kakizaki diabetic rat model, and the
effects of salidroside” (Alix Montloin and Anne-Sophie Leguay)

July-August 2009: Institute of Physiology, Minsk, Belarus

Supervising undergraduate students work (Larisa Lopatina and Vladimir Kazbanov) in frames
of the project “The role of circulating factors in vascular dysfunction induced by physical
inactivity and microgravity in rat models of tail suspension and antiorthostatic hypokinesia”

Current scientific interests

Physical inactivity and simulated microgravity

Cardiovascular deconditioning
Microcirculation
Endothelial dysfunction
Diabetes, metabolic disorders
Endotoxin translocation

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 Grants, fellowships, awards

2006 Grant of ECO-NET program co-financed by CNES (French Space Agency) for
1-month stage at the Vascular Investigation Department of Angers University
Hospital, France

2007- PhD fellowship from the French Embassy in Belarus for 18 months
2010

2008 Award at the Forum of PhD students of the University of Angers for the best
poster presentation

2010- Postdoctoral grant of the Regional Council of Pays de la Loire

2012

Participation in scientific conferences

2008 International meeting “Life in Space for Life on Earth”, Angers, June 22-27

2009 International scientific-practical conference “Science for innovative development of

society”, Minsk, January 22-23

2009 Congress of Physiology, Pharmacology and Therapeutics, Marseille, April 15-17

2009 Second Congress of Physiological Sciences of Serbia with international participation

“Current Trends in Physiological Sciences”, Kraguevac, September 17-20 (invited talk)

2009 Doctoral forum of Biology-Health Doctoral School, Nantes, November 12

2010 International conference “Neurovascular impairment induced by environmental

conditions: molecular, cellular and functional approach”, Angers, March 10-14

2011 International ASGSB /ISGP Congress, San Jose, USA, November 2-6

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 Publications

2008
1. Novoselova AM, Custaud MA, Tsvirkun DV, Larina IM, Kulchitsky VA. Metabolism in rats during
antiorthostatic hypokinesia. Bulletin of Experimental Biology and Medicine 146(1):38-40, 2008.
2. Navasiolava NM, Pashkevich SG, Custaud MA, Emelianova AA, Kulchitsky VA. Response to LPS
injection after anti-orthostatic hypokinesia in rats, an hypothesis for endothelial dysfunction.
Journal of Gravitational Physiology 15(1):69-70, 2008.
3. Navasiolava NM, Pashkevich SG, Emelianova AA, Custaud MA, Kulchitsky VA. Ultrastructural changes
in brain vessels during antiorthostatic hypokinesia. Neuroscience: theoretical and clinical aspects
(Ukraine) 4(2):21-24, 2008.
4. Emelianova AA, Pashkevitch SG, Navasiolava NM. The particularities of rat brain vessels ultrastructure
after two weeks of antiorthostatic hypokinesia // The problems of regulation of visceral functions.
NASB, Anthology of scientific papers in two volumes. Minsk. Vol 1:24-27, 2008.

2009
5. Martusevich NO, Pashkevich SG, Navasiolava AM, Patseev SV, Custaud MA, Kulchitsky VA. The
features of endotoxin translocation process in human and animal organisms. News of biomedical
sciences 3:7-11, 2009.
6. Navasiolava N, Custaud MA. What are the future top priority questions in cardiovascular research
and what new hardware needs to be developed? Respiratory Physiology & Neurobiology 169 Suppl
1:S73-74, 2009.

2010
7. Navasiolava NM, Dignat-George F, Sabatier F, Larina IM, Demiot C, Fortrat JO, Gauquelin-Koch G,
Kozlovskaya IB, Custaud MA. Enforced physical inactivity increases endothelial microparticle levels in
healthy volunteers. American Journal of Physiology 299:H248-256, 2010.

2011
8. Navasiolava NM, de Germain V, Levrard T, Larina IM, Kozlovskaya IB, Diquet B, Le Bouil A, Custaud MA,
Fortrat JO. Skin vascular resistance in the standing position increases significantly after 7 days of dry
immersion. Autonomic Neuroscience: Basic and Clinical 160:64-68, 2011.
9. Navasiolava N, Custaud MA, Tomilovskaya E, Larina I, Mano T, Gauquelin-Koch G, Gharib C,
Kozlovskaya I. Long Term Dry Immersion: Review and Prospects. European Journal of Applied
Physiology 111:1235-1260, 2011.
10. Navasiolava NM, Custaud MA. Microgravité et vieillissement vasculaire [Microgravity and vascular
aging]. Biofutur 318 : 30, 2011.
11. Navasiolava N, Pajot A, Gallois Y, Pastushkova L, Kulchitsky V, Gauquelin-Koch G, Kozlovskaya I, Heer
M, Hand O, Larina I, Custaud MA. NT-ProBNP levels, water and sodium homeostasis in healthy men:
effects of 7 days of dry immersion. European Journal of Applied Physiology 111:2229-2237, 2011.
12. Larina IM, Baevsky RM, Pastushkova LKh, Navasiolava NM, Custaud M-A, Eshmanova AK, Luchitskaya
ES. Seven-day dry immersion: relations between water-electrolyte and cardiovascular responses.
Fiziol Cheloveka 37:100-107, 2011.
13. Novoselova AM, Strizhak IV, Custaud MA, Larina IM, Pashkevich SG, Kulchitsky VA. Толерантность к
эндотоксину у крыс в условиях антиортостатической гипокинезии [State of endotoxin tolerance
in rats in conditions of antiorthostatic hypikinesia] // Proceedings of the National Academy of
Sciences of Belarus [Vestsi Natsiyanalnai Akademii Navuk Belarusi], Series of Medical Sciences 4: 24-
28, 2011.
14. Noskov VB, Larina IM, Pastushkova LKh, Dobrokhotov IV, Valeeva OA, Coupé M, Custaud MA,
Novoselova AM. Kidney activity and the state of body liquids in human in dry immersion conditions
[Деятельность почек и состояние жидкостных сред организма человека в условиях «сухой
водной иммерсии]. Aviakosm Ekolog Med 45:22-26, 2011.

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 2012
15. Tsvirkun D, Bourreau J, Mieuset A, Garo F, Vinogradova O, Larina I, Navasiolava N, Gauquelin-Koch G,
Gharib C, Custaud MA. Contribution of social isolation, restraint, and hindlimb unloading to changes
in hemodynamic parameters and motion activity in rats. PLoS ONE (accepted).
16. Coupe M, Navasiolava N, Alameddine A, Gauquelin-Koch G, Gharib C, Custaud MA. How to prevent
vascular and microvascular dysfunctions induced by physical inactivity and microgravity? (under
preparation).
17. Yuan M, Coupe M, Navasiolava N, Li Y, Gauquelin-Koch G, Bai Y, Jiang S, Ayer A, Wan Y, Henrion D,
Yinghui Li, Custaud MA. ES-IBREP: Effect of Chinese herbal medicine on vascular functions during 60-
day head-down bed rest. (under preparation).
18. Coupe M, Tomilovskaya E, Gallois Y, Diquet B, Kozlovskaya IB, Larina IM, Gauquelin-Koch G, Kulchitsky
VA, Custaud MA, Navasiolava NM. Body fluid changes, cardiovascular deconditioning and metabolic
impairment are reversed 24 hours after a 5-day dry immersion. (under preparation).

Communications

2008
1. Navasiolava NM, Pashkevich SG, Custaud MA, Emelianova AA, Kulchitsky VA. Response to LPS
injection after anti-orthostatic hypokinesia in rats, a hypothesis for endothelial dysfunction //
International meeting “Life in Space for Life on Earth”, Angers, June 22-27, 2008. Oral presentation
2. Navasiolava N.M., Tsvirkun D.V., Pastushkova L.Kh., Larina I.M., Dobrokhotov I.V., Custaud M.-A.,
Fortrat J.O., Gharib G., Gauquelin-Koch G. Skin microcirculatory dysfunction induced by 7 days of dry-
immersion // International meeting “Life in Space for Life on Earth”, Angers, June, 2008. Oral
presentation
3. Blossier JD, Tsvirkun D, Navasiolava N, Deschamps- Bourreau J, Custaud M.A, Larina I, Demiot C.
Cardiovascular changes induced by 7 days of dry immersion // International meeting “Life in Space
for Life on Earth”, Angers, June, 2008. Poster
4. Navasiolava N.M., Custaud M.-A., Pashkevich S.G., Patseev S.V., Kulchitsky V.A. Эндотелиальная
дисфункция в условиях антиортостатической гипокинезии – причина или следствие
транслокации эндотоксина из кишечника в кровь? [Endothelial dysfunction in the conditions of
antiorthostatic hypokinesia – the reason or the consequence of endotoxin translocation from the gut
to the blood?] // The sixth Russian meeting with international participation “The mechanisms of
functioning of visceral systems”, Sanct-Petersbourg, September 30 -October 2, 2008. Oral presentation
5. Roeva MO, Kvacheva ZB, Tsedik LV, Il’yutschenko AF, Chernov AN, Navasiolava AM, Custaud MA,
Patsaev SV, Pashkevich SG, Kalunov VN, Kulchitsky VA. Пролиферативная активность фибробластов
в керамических конструкциях при моделировании микрогравитации [Proliferate activity of
fibroblasts in ceramic constructions in simulated microgravity] // The second meeting of physiologists
of the Commonwealth of Independent States, Kishinev, October 29-31, 2008. Oral presentation

2009
6. Navasiolava NM, Kulchitsky VA, Larina IM, Custaud MA. Microcirculatory dysfunction induced by
environmental conditions and simulated weightlessness // International scientific-practical
conference "Science for innovative development of society ", Minsk, January 22-23, 2009. Oral
presentation
7. Navasiolava N, Dignat-George F, Sabatier F, Larina I, Demiot C, Fortrat JO, Gauquelin-Koch G,
Kozlovskaya I, Custaud MA. Les fonctions endothéliales sont rapidement altérées par la microgravité
simulée [Endothelial functions are rapidly impaired by simulated microgravity] // Congress of
Physiology, Pharmacology and Therapeutics, Marseille, April 15-17, 2009. Oral and poster
presentations

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8. Navasiolava N, Pajot A, Gallois Y, Kulchitsky VA, Gauquelin-Koch G, Pastushkova LKh, Larina IM,
Custaud MA. NT-proBNP level, water and sodium metabolism: effects of 7 days of dry immersion //
The seventeenth IAA Human in Space Symposium, Moscow, June 7-11, 2009. Oral and poster
presentations
9. Kulchitsky VA, Custaud MA, Navasiolava NM, Pashkevich SG, Roeva MO, Emelianova AA.
Microgravitation and development: positive and negative questions // The second Congress of
Physiological Sciences of Serbia with international participation "Current Trends in Physiological
Sciences", Kraguevac, September 17-20, 2009. Invited oral presentation
10. Roeva MO, Drozd ES, Chernov AN, Chizhik SA, Navasiolava AM, Custaud MA, Kulchitsky VA.
Особенности ультраструктуры фибробластов в условиях изменения действия гравитационного
фактора [The particularities of fibroblasts’ structure under the conditions of the change of action of
gravitational factor] // International scientific conference “Patterns of development of pathological
states and their correction”, Minsk, October 27-28, 2009. Oral presentation
11. Navasiolava N, Dignat-George F, Sabatier F, Larina I, Kulchitsky V, Fortrat JO, Gauquelin-Koch G,
Kozlovskaya I, Custaud MA. Les fonctions endothéliales sont rapidement altérées par la microgravité
simulée // Doctoral forum of Biology-Health Doctoral School, Nantes, November 12, 2009. Oral
presentation

2010
12. Navasiolava NM, Custaud MA, Larina IM, Kulchitsky VA. Metabolism intensity in rats during
antiorthostatic hypokinesia // International conference “Neurovascular impairment induced by
environmental conditions: molecular, cellular and functional approach”, Angers, March 10-14, 2010.
Oral presentation
13. Navasiolava NM, de Germain V, Levrard T, Larina IM, Kozlovskaya IB, Diquet B, Le Bouil A, Custaud MA,
Fortrat JO. Dry immersion increases skin vasoconstriction to standing // International conference
“Neurovascular impairment induced by environmental conditions: molecular, cellular and functional
approach”, Angers, March 10-14, 2010. Oral presentation
14. Kulchitsky VA, Navasiolava NM, Patzeev SV. Weightlessness and systemic inflammatory process: the
ways to prevent endothelial dysfunction // International conference “Neurovascular impairment
induced by environmental conditions: molecular, cellular and functional approach”, Angers, March 10-
14, 2010. Oral presentation

2011
15. Pastushkova LKh, Eshmanova AK, Luchitskaya ES, Custaud M-A, Navasiolava N M, Larina IM, Baevsky
RM. Water-electrolyte, hormonal and vegetative balance during 24-hour immersion // 18th IAA
Humans in Space Symposium, Houston, Texas, April 11–15, 2011. Poster presentation
16. Pastushkova LKh, Larina IM, Coupé M, Gauquelin-Koch G, Kulchitsky VA, Custaud MA, Navasiolava NM.
Влияние 5-суточной сухой иммерсии на состояние жидкостных секторов организма человека
[L’influence d’immersion sèche de 5 jours sur l’état de secteurs liquidiens de l’organisme humain] //
Space forum-2011, Star City, Russia, Octobre 18-21, 2011. Oral presentation
17. Navasiolava NM, Coupé M, Kulchitsky VA, Larina IM, Pastushkova LKh, C. Gharib, and MA. Custaud.
Effect of 5 days dry immersion on body fluids in healthy volunteers // ASGSB /ISGP Joint Meeting,
San Jose, California USA, November 2-6, 2011. Oral presentation
18. Coupé M, Navasiolava NM, Levrard T, Tomilovskaya ES, Gauquelin-Koch G, Kozlovskaya IB, Custaud
MA. Orthostatic response immediately and 24 hours after a 5 day dry immersion experiment //
ASGSB /ISGP Joint Meeting, San Jose, California USA, November 2-6, 2011. Oral presentation
19. Levrard T, Coupé M, de Germain V, Navasiolava NM, Gauquelin-Koch G, Custaud MA. Televasc, online
software and custom services analysis platform // ASGSB /ISGP Joint Meeting, San Jose, California
USA, November 2-6, 2011. Oral presentation

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 Scientific contacts
Institute of Biomedical Problems, Moscow, Russia (IBMP) - Pr Irina Larina & Pr Inesa Kozlovskaya
China Astronaut Research and Training Center, Beijing, China (ACC) - Dr Ming Yuan
Laboratory of Environmental Physiology, Lyon, France - Pr Claude Gharib
Institute for space medicine and physiology, Toulouse, France (MEDES) - Dr Marie Pierre Bareille
French Space Agency, Paris, France (CNES) - Dr Guillemette Gauquelin-Koch
Gifu University of Medical Science, Japan - Pr Tadaaki Mano
Vascular Aging and Space Research Program Laboratory, Canada - Pr Richard Hughson

Additional skills

Computer: confident user. Microsoft Office, Internet, Acknowledge, SPSS, Prism

Languages: fluent in English and French; basic knowledge of German, Spanish and
Japanese; native languages – Russian and Belarusian
Drawing: manuscript figures, logotype, announcement for congress etc

Hobbies & Interests

Reading, cycling, hiking, chess, table tennis

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 References

Marc-Antoine CUSTAUD
MD, PhD
Maître de conférences des Universités-Praticien hospitalier (HDR)
CNRS UMR 6214 - INSERM U1083, Angers Faculty of Medicine, Haute de Reculée Street,
49045, Angers Cedex, France
Cell: 33 (0) 6 07 11 13 63
Office: 33 (0) 2 41 35 36 89
Fax: 33 (0) 2 41 35 50 42
[email protected]

Claude GHARIB
MD, PhD, Professor Emeritus
Laboratory of Environmental Physiology, Grange Blanche Faculty of Medicine, Claude
Bernard University Lyon 1, 69373 Lyon Cedex 08, France
Tel: +334 78 77 70 78
[email protected]

Tadaaki MANO
MD, PhD, Professor
Gifu University of Medical Science, 791-1 Nagamine Ichihiraga, Seki, Gifu 501-3892, Japan
Tel: +81-575-22-9401 ext.201
Fax: +81-575-25-2590
[email protected]

Irina Mikhailovna LARINA

MD, PhD, Dr.Sci., Professor
Institute of Biomedical Problems (IBMP), Russian Academy of Sciences, 76A Khorochevkoe
Shosse, 123007, Moscow, Russia
Tel: +7 499-195-65-28
[email protected]

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