22/05/2024 14:15:26

Copy of pAX01_T7Pol (10808 bp)

pG2_For (DNA),ScaI,EarI pMBP-LbCpf1 REV (DNA),ZraI,AatII,BclI,BsmBI,+2

PvuI PacI
Rev_A'_cotY (DNA),+2 TspMI
XmaI
SmaI,Eco53kI,BanII,+3
AjuI
00 StuI
100 pBE-S_RE
V (D p PaeR7I,TliI,+1
NA) AX01
_L EcoRI,SalI
bC
7 'LacA pf
DrdI 964 AmpR 1 BseRI
) 5 9- Amp-R lam F
PciI 90 bd
a
NA

WD
BspQI,+2 t0 PsiI
(D

(D
PflFI,+2 Xy te
er

NA
l
im

-F

)
DrdI SpeI

rm
i

ri
or
_Pr

R
in
BsmBI,+2 2o BamHI
32 er

at
X_3

or
- m BR

k
3'
pGE

PCR p
p
ar
bom

X
A m p R yl
X
rop

0
pGE

pRS

A)
Pxyl_T7Pol_F (DN )
pr
44

A
o

PxylA_rev (DN
L4

+1

XylA Promoter
mo

ajout
AfeI

2500
ter
+1
l a m b d a t 0 2 ter rmi

+1
PCR_Ori_

MscI 10808 bp +1
+1 +1
rrnB

l a c A m i n a i nat to

BL21
+3 PaqCI
rrnB

col

07
'
ter

+4 +1
T

T7p

Pol
BsrGI

1
T1

479
T7 Bsu36I
750

te
m
t o or

40-
BstXI
r

R
0

PC
rrn

SexAI 21
na

pBR
B-

r forEco
NcoI
T1

T7 terminator
_R

EagI,+1 erm
ev

PC
BanII NA R
Ter tet pr T7 Term
(D

EagI,NotI ) min omoter Stop HpaI

ateu
PacI r pBMP
BglII pJM
P1 F
HpaI WD (DNA)
TspMI,XmaI,SmaI,+1 BbvCI
BstZ17I 5000
XcmI,SnaBI BsrGI,Acc65I,KpnI
BaeI T7Pol_MluI_R (DNA),+5
ScaI Rev_T7_term (DNA)
pBE_term_R (DNA),pMBP-LbCpf1 REV (DNA),+5

https://benchling.com/abrans/f/lib_UrEiwxL8-promoteur/seq_vD1kcJ0x-copy-of-pax01_t7pol/edit 1/1