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 Form 1 (FOR OFFICE USE ONLY)
THE PATENTS ACT, 1970
(39 of 1970) Application No:..
& Filing Date...
THE PATENT RULES, 2003 Amount of Fee paid:..
APPLICATION FOR GRANT OF PATENT CBR No:.
|See sections 7,54 & 135 and rule 20(1)] Signature:...
1. APPLICANT(S)

Sr. Name Nationality Address

Ms.B.MONISHA India
Sri Shakthi Institute of Engineering &
1. Technology, Coimbatore, 641062
2. INVENTOR(S)

Sr. Name Nationality Address

India
Sri Shakthi Instituteof Engineering & Technology,
Ms.NANDHINIG Coimbatore, 641062
India
Sri Shakthi Institute of Engineering & Technology,
2 Ms.NANDHINIR Coimbatore, 641062

Ms.MONICA S India
Sri Shakthi Institute of Engineering &Technology,
3
Coimbatore, 641062
India
Sri Shakthi Institute of Engineering & Technology,
4 M.ADHISH R K
Coimbatore, 641062
Ms.MONISHA B India
Sri ShakthiInstitute of Engineering &Technology,
5
Coimbatore, 641062

3.TITLE OFTHE INVENTION: ECO-FRIENDLY DEGUMMING TREATMENT USING

PECTINASE AND XYLANASE IN TEXTILE INDUSTRIES

4. ADDRESS FOR CORRESPONDENCE OF APPLICANT /

Mobile No.:9600288370
AUTHORISED PATENT AGENT IN INDIA
E-mail: [email protected]

1. Ms.B.Monisha, Department of Biotechnology

Sri Shakthi Instituteof Engineering &Technology
 5.PRIORITY PARTICULARSOF THE APPLICATION(S) FILED IN CONVENTION COUNTRY:
Sr. Country Application Number Filing Date Nameof the Applicant Titleof the invention

6. PARTICULARS FOR FILING PATENT COOPERATION TREATY (PCT) NATIONAL PHASE

APPLICATION

International Filing Date as Allotted by the

International Application Number Receiving Office
PCTI/ / 01/01/190000:00:00

7. PARTICULARS FOR FILING DIVISIONAL APPLICATION

Original (first) Application Number Date of Filing of Original(first) Application
01/01/1900 00:00:00
8. PARTICULARS FOR FILING PATENT OF ADDITION

Main Application / Patent Number Dateof Filing of Main Application

PCTI/ / 01/01/1900 00:00:00

9. DECLARATIONS:

) Declaration by the inventorss) Ms.G.NANDHINI, Ms.R.NANDHINI, Ms.S.MONICA,

Mr.R.K.ADHISH and Ms.B.MONISHA'are the true &first inventor(s) for this Invention and declare
that the applicant(s) herein /e/are my/oyr assignee or legal representative.
(a) Date

Sr. Name SIGNATURE WITH DATE

Ms.NANDHINIG NhG a3/06/2023

2 Ms.NANDHINI R
3 Ms.MONICA S

Mr.ADHISH RK RKAU23 lo 6/2023

 5 Ms.MONISHAB

(ii) Declaration by the applicant(s)

Weare, in possession of the above-mentioned invention.
There is no lawful groundof objection to the grant of the Patent to me/us.

WITH THE APPLICATION:

10. FOLLOWING ARE THE ATTACHMENTS
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Sr. Document Description
attached
Complete Specification
attached
2. Supporting Tables

belief the fact and

Dr.J.BINDHUhereby declare that to the best of my knowledge, information and invention.
I patent may be granted to me/us for the said
request that a
matters stated herein are correct and I

Dated this (Final Payment Date): 23lo6l2023. Signature:o

DD-No 981654321.
To The Controller of Patents
The Patent Office at Chennai

This form is electronically generated

 THE GAZETTE OFINDIA:EXTRAORDINARY PART II-Sec. 3(i0)]

FORM 2
THE PATENT ACT 1970
(39 of 1970)
&
The Patents Rules, 2003
PROVISIONAL/COMPLETE SPECIFICATION
(See section 10 and rule13)

1. TTLE OF THE INVENTION Eco-derdly daguhnriing drecdmort ng

Rchraue nd ulanase pn teoettlo hcuste.
2.
APPLIGANT (nha
(a) NAME:Ms. B
(b) NATIONALITYIrdFe)
ot Brechncly, GPshakta ua of
() ADDRE^S:lopqrtmet
OngnLf ahd techndlsCo foobetoe
3. PREAMBLE Ro THE DESCRIPTION
PROVISIONAL COMPLETE
The following specification describes the The following specificatioh particulady describes
invention. the invention and th¿ manner in which it is to be
performed.

4. DESCRIPTION {Description shall start from next page.)

Ateched
5. CLAIMS (not applicable for provisional specification. Claims should start with the preamble
/we claim" on separate page)
Attachd
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7. ABSTRACT OF THE INVENTION (to be given alongwith complete specification on separate

page)
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Note:
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country.
"Strike out the column whlch ls/are not applhcable
 FORM 3
THE PATENTS ACT, 1970
(39 of 1970)
&
THE PATENTS RULES, 2003
STATEMENT AND UNDERTAKING UNDER SECTION8
(See Section 8; rule 12)
1. Namely of the applicant(s). I/We,..Montdha.P.
hereby declare:
2 Name, address and nationality of () that I/We have not made any application for
the joint applicant the same /substantially the same invention
outside India.
Or
(i) that 1/Wwho
No........
have made this application
dated. .alone
ljointly withk. made
for the same \substantially same invention,
application(s)\for patent in the other countries,
the particulars pf which are given below:
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(iii) that thà rights in the application(s) has /have

been assigned
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that I/We undertake that upto the date of grant

of the patent hy the Controller, I/We would
keep him inforned in writing the detaiis
regarding correxponding applications for
patents filed outside lIndia within three months
from the date of ing of such application.
Dated this... ...day of. ......2.0.

4. To be signed by the applicant or

his authorised registered patent Signature.
agent
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has signed C.Ma.Man£dha.B.. ....
To
The Controller of Patents,
The Patent Office,
At.Chunna..
Note. Strike out whichever is not applicable
 (HPT I|-es 3()] 87

FORM5
THE PATENTS ACT, 1970
(39 of 1970)
&
The Patents Rules, 2003
DECLARATION AS TO INVENTORSHIP
[See section 10(6) arnd rule 13(6)]

1. NAME OF APPLICANT (S)

Ms. Mornha b
hereby declare that the true and first inventor(s) of the invention disclosed in the complete
specification filed in pursuance of my lour application numbered
dated islare
2. INVENTOR (S),
(a) NAME Aheh k k. Monfa. S, Nardk.a, Alandk-R g Mongha
(b) NATIONALITYIidn
(c) ADDRESsDpovtrentot Btehnagy, Sf shakeT hetute of Ergmaeofing6
Dated this.......2...day of...Jne.....20.2.....
Tachrcingy.
Signature: -
Name of the signatory: -

Sr. Name SIGNATURE WITH DATE

1 Ms.NANDHINIG
Nli.G/ 23/o6|ab23
2 Ms.NANDHINIR
3 Ms.MONICA S
4 Mr.ADHISHRK
Rk A 23106/ 2023
5 Ms.MONISHAB
 3. DECLARATION TO BE GIVEN WHEN THE APPLICATION IN INDIA IS FILED BY THE
APPLICANT (S) IN THE CONVENTION COUNTRY: -
We the applicant(s) in thà convention country hereby declare that our right to apply for a patent in
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Dated this day of.... 20..... .......

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IWe assent to the invention referred to in the above
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Dated this ..day of......... 20...... .....

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Note
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registered patent agent othaerwise where
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 FORM 9
THE PATENTS ACT, 1970
(39 of 1970)
&
THE PATENTS RULES,2003
REQUEST FOR PUBLICATION
[Seesection 11A (2); rule 24A]

1. Name, address and nationality of Iy..s:Manisha...B....

applicant(s).
hereby request for early Publication of mylour
Patent application No.
dated. under section 11A(2) of
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Dated this.....23.day of.. June..20.23..

2. To be signed by the applicant or
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agent. Signature

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has signed.

To
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At..Chenna
Note. For fee: See First Schedule,
 THE PATENTS ACT, 1970
PROVISIONAL
SPECIFICATION
SECTION 10
TITLE
ECO-FRIENDLY DEGUMMING TREATMENT USING PECTINASE AND XYLANASE
IN TEXTILE INDUSTRIES

APPLICANT
B Monisha

INVENTORS

Adhish RK,Monica S, Nandhini G, Nandhini R &BMonisha

Department of Biotechnology, Sri Shakthi Institution of Engineering &Technology.
Coimbatore, TamilNadu, India.

TITLE

Eco-friendly degumming treatment using pectinase and xylanase in textile industries.

FIELD OF INVENTION

The invention aiscloses the major concern of this project is the degumming of natural fibres
by an eco-friendly method. Degumming of fibre can be performed by hemicellulose
degrading enzymes such as Pectinase and Xylanase. Both are easily available and easy to
manufacture. Hence they can be easily extracted from agricultural waste (rice straw, soybean
residues, malt sprout, etc..). The texiile industry plays a significant role in global
manufacturing, but its operations often result in negative environmental impacts due to the
use of harsh chemicals and high energy consumption. This study proposes an eco-friendly
degumming treatment for textile industries using pectinase and xylanase enzymes.
METHODOLOGY

Submerged fermentation: Preparation of media (Nutrient broth prepared for 100ml

in each). Inoculation of microorganisms (Pectinase: Pseudomonas fluorescens;
Xylanase: Bacillus subtilis). Submerged fermentation (Incubation at 37°C for 4-5 days
with 140 rpm).
Ultrafilteration: Then the media with is filtered with muslin cloth.
Enzyme assay for xylanase and pectinase: Lignin content is calculated at
wavelength of 280nm and fiber free filtrates was calculated using UV visible
spectrophotometer.
Assay for Pectinase: Pectinase enzyme is taken and diluted with 0.1IM citrate buffer
(pH 5.0) at 45°C for 30 mins. Using viscometric method the viscosity is estimated and
then cellulase are measured through filter paper. This is repeated for three times.
Assay for Xylanase: Xylanase enzyme is taken and diluted with 0.05M citrate buffer
(pH 5.0) at 50°Cfor 15 mins. Then incubated the reaction is stopped by addition of
1.75 ml of 3,5 dinitrosalicylic acid and in boiling bath heated for 10 mins. The
liberated sugars were measured under spectrophotometrically at 550nm.
Enzymatic degumming method of ramie fibre: The raw ramie fiber is taken as
small bundles. The ramie bundles were opened to the degumming process before that
the ramie fiber is washed with water containing non-ionic emulsifier for 2-3 hrs. Then
the fiber is dipped into degumming bath and incubated. Then the fiber is thoroughly
washed with hot and cold water. This is repeated for three times. Optimation time of
incubation is 4-72 hrs at 37-55°C.
Scanning electron microscopic study: The Xylanase treated sample and Pectinase
treated sample are combined sample were take and observed in the scanning electron
microscope .

Matse
DESCRIPTION

Degumming process: Degumming of silk involves the cleavage of peptide bonds of

sericin, either by hydrolytic or enzymatic methods, and the subsequent removal of
sericin from the silk fibroin. Hydrolysis of sericin can be carried out under neutral,
alkaline or acidic conditions to give four fractions, each having different properties. In
traditional textile processing, degumming is an essential step for removing sericin and
pectin from silk and plant-based fibres, respectively. However, conventional
degumming methods often involve the use of harsh chemicals and high energy
consumption, leading to environmental pollution and resource depletion. The eco
friendly degumming treatment using pectinase and xylanase offers a sustainable
alternative.
Pectinase: Utilization of microbial pectinase in different industries has been increased
in its world demand. The major sources of pectinase are microorganisms mainly
bacteria, fungi and yeast. The utilization of low-cost agro-industrial wastes as
production faced
substrates has been preferable in pectinase production. Pectinase
production
various parameters optimization constraints such as temperature, pH and
is
times which are the main factors in pectinase production. The pectinase enzyme
to be explored further
getting attention due to its several advantages; hence, it needs
This review discusses the
to take its maximum advantage in different industries.
production, factors influencing
pectin substance structure, substrate for pectinase
microbial pectinase and also
pectinase production, the industrial application of
microbial pectinase in
discusses challenges and future opportunitis of applying
a complex
industry. Pectinase is an enzyme that specifically targets pectin,Pectin acts as
carbohydrate found in plant-based fibres such as cotton, flax, and hemp.
resistant to separation. By
a natural glue, binding fibres together and making them
simpler components,
applying pectinase, the pectin molecules are broken down into
impurities.
facilitating the separation of fibres and the removal of
occurring renewable
Xylanase: Xylan is the second most abundant naturally
consisting of
polysaccharide available on earth. It is a complex heteropolysaccharide organic
D-manno es and
different monosaccharides such as L-arabinose, D-galactose,
interwoven together with help
acids such as acetic acid, ferulic acid, glucuronic acid
of xylan is restricted due to its
of glycosidic and ester bonds. The breakdown
xylanases which are capable of
heterogeneous nature and it can be overcome by
are abundantly present
cleaving the heterogeneous B-1,4-glycoside linkage. Xylanases
and several microorganisms
in nature (e.g., molluscs, insects and microorganisms) extensively for its production.
such as bacteria, fungi, yeast, and algae are used
Microbial xylanases show varying substrate specificities and biochemical properties
industrial and biotechnological
which makes it suitable for various applications in
in food and feed, paper and
sectors. The suitability of xylanases for its application
biorefinery has led to an increase in
pulp, textile,pharmaceuticals, and lignocellulosic gives an insight of using microbial
demand of xylanases globally. The present review future
with its current status and
xylanases as an "Emerging Green Tool" along major
prospective. Xylanase is an enzyme that effectively degrades xylan, a
 component of hemicellulose found in plant-based fibres. Hemicellulose contributes to
the stiffness and brittleness of fibres, making them dificult to process. By employing
xylanase, the hemicellulose structure is degraded, resulting in improved fibre
flexibility and increased accessibility to other treatments.
Plant materials

Cotton: Cotton is a widely used plant-based fibre in the textile industry known for its
softness, breathability, and absorbency. It is derived from the fluffy fibres that
surround the seeds of the cotton plant (scientifically known as Gossypium). The
cotton fibre consists mainly of cellulose, a complex carbohydrate that forms the
structural component of the cell walls in plants. Cellulose accounts for approximately
90% of the cotton fibre composition. The remaining 10% comprises various
substances, including proteins, pectin, hemicellulose, waxes, and minerals. Cellulose
molecules,
is the primary component of cotton fibres. It forms long chains of glucose
providing strength and structure to the fibres. Cotton fibres contain small amounts of
proteins, such as globulins and albumins. These proteins can contribute to the overall
strength and elasticity of the fibres. Pectin is a complex polysaccharide that acts as a
cementing material in the cell walls of plants. In cotton fibres, pectin helps bind the
cellulose fibres together, contributing to their cohesion and resistance to separation.
Hemicellulose is another polysaccharide present in cotton fibres. It surrounds the
cellulose chains, providing additional support and rigidityto the fibres. Hemicellulose
is one of the targets for degradation during the eco-friendly degumming treatment
using xylanase. Cotton fibres also contain natural waxes, which provide a protective
fibre's
coating on the fibre surface. These waxes help repel water and contribute to the
moisture resistance. Trace amounts of minerals, such as calcium, potassium, and
magnesium, are present in cotton fibres. These minerals are essential for plant growth
fibres.
and are typically found in the plant's vascular system, which includes the
Jute: Jute is a natural fibre derived from the plants of the Corchorus genus, primarily
Corchorus olitorius and Corchorus capsularis. It is commonly known as the "golden
fibre" due to its golden colour and is widely cultivated in regions with a warm and
humidclimatc, such as Bangladesh and India. Jute fibres are known for their strength
and
and durability, similar to bamboo fibres. Jute is one of the strongest natural fibres
has high tensile strength, making it suitable for various applications. Jute fibres are
highly versatile and can be used in a wide range of products. They are commonly used
textiles. Jute is
to make sacks, bags, ropes, twine, carpets, rugs, and various types of
considered an eco-friendly fibre because it is biodegradable and renewable. It has a
low carbon footprint and is often used as a sustainable alternative to synthetic
materials. Jute fibres have good breathability, allowing air to flow through the fabric.
fibres have
This makes jute products comfortable to use in warm climates. Jute
excellent moisture absorbency, which makes jute sacks and bags ideal for packaging
agricultural products like grains, fruits, and vegetables. Jute fibres have natural
insulating properties, providing thermal insulation against heat and cold. Jute carpets
and rugs can help regulate room temperature and reduce energy consumption. Jute
fibres are naturally fire-resistant, which makes jute products safer in certain
applications. Jute fibres are susceptibleto damage from prolonged exposure to
moisture and sunlight. They can also be weakened by certain chemicals. Proper care
and maintenance are important to preserve the quality and longevity of jute products.
Jute fibres have been used for centuries in various industries, including agriculture,
packaging, textiles, and construction. They are known for their affordability,
versatility, and environmentally friendly nature. The demand for jute products has
sustainable and
increased in recent years as there is a growing emphasis on
biodegradable materials.
Bamboo: Bamboo is a versatile plant that has been used for various purposes for
growth and
centuries. It belongs to the grass fanmily and is known for its fast
sustainability. Bamboo fibres, derived from the stalks of bamboo plants, have gained
eco-friendliness. Bamboo
popularity in recent years due to their unique properties and
hardwoods like
fibres are known for their strength and durability, often compared to
oak. This makes bamboo an excellent choice for various applications, including
textiles and construction materials. Bamboo fibres have a natural softness, making
them comfortable to wear. Bamboo fabric is often described as silky and luxurious,
with atexture similar to cashmere or silk. Wicking and Breathability: Bamboo fibres
have excellent moisture-wicking properties, meaning they can absorb and evaporate
moisture quickly. This makes bamboo fabrics higlhly breathable, keeping the wearer
cool and dry. Bamboo fibres have natural thermo-regulating properties, helping to
keep the body warm in cold weather and cool in hot weather. This makes bamboo
clothing suitable for different climates and seasons. Bamboo fibres contain a natural
antimicrobial bio-agent called "bamboo kun." This makes bamboo fabrics resistant to
bacteria, fungi, and other microbes, reducing odour and preventing allergic reactions.
Bamboo is a highly sustainable and renewable resource. It grows rapidly, requires
minimal water, and doesn't need pesticides or fertilizers. Additionally, bamboo plants
release more oxygen and absorb more carbon dioxide compared to other trees.
Bamboo fibres can be processed into various forms, including yarns, fabrics, and
composite materials. They can be blended with ther fibres like cotton, hemp, or
polyester to enhance specific characteristics. While bamboo fibres offer many
advantages, the manufacturing process to extract and process the fibres can involve
the use of chemicals. It's important to ensure environmentally friendly production
practices when working with bamboo fibres.
METHODOLOGY:

Isolation of culture strain(Pectinaseand Xylanase)

Preparation and optimizationof culturemedia

Submerged fermentation(Incubation for 4-5 days at 37°C, 140 rpm)

Ultrafilteration

Enzyme assay for pectinase and xylanase

Enzymatic degumming method of ramie fiber

Scanning electron microscopic study

DISCLOSURE

Reference may be made to a patent no:

the title Enzyme composition for WO2022003635A1 Chowdhary Rashmi et al., with
converting plant biomass into high quality textile grade
fibre". The current invention discloses
enzyme-based compositions for converting raw
natural fibres from plant derived biomass into high quality textile grade fibres. The invention
discloses at least one multi-component enzymatic formulation, and the optimal conditions for
using these enzymatic formulations, which result in
production of textile grade fibres from
raw natural fibres. These textile grade fibres can be used in any
industry, because of their
high-quality parameters, and high spinnability index.
Reference may be made to a patent no: WO2022003638Al by Chowdhary Rashmi et al.,
with the title"Method of enzymatic processing of plant biomass to produce textile grade
fibre". The current invention discloses an enzyme- based method for making high quality
textile fibres from plant derived biomass. The invention discloses a method for
production of
high-quality textile grade fibres that have no loss in quality parameters as compared to textile
grade fibres made from cellulosic biomass by conventional methods that use harsh chemical
treatments. The fibres produced by this method from raw natural fibres can be spun into yarn
by automated procedures and the yarncan be woven into high quality abrics by power loom
as well as handloom.

Reference may be made to a patent no:CN104342407A by Anjan Kumar Dasgupta et al.,

with the title "Psychrophilic enzymes compositions and methods for making and using
same". Enzyme compositions with enhanced enzyme activity and/or thermophilic and
psychrophilic stability are described. Additionally, methods and kits for making and using the
enzyme compositions are provided.
Reference may be made to a patent no: WO2023 002507A1 by SirupaHarshaavardhan Redi
et al., with the title "Eco- friendly bioprocess for separation of hemp plant fibres for various
applications". An eco-friendly bioprocess for separation of hemp plant fibres, from
decorticated hemp fibres skin, said process comprising: pre-treating the decorticated hemp
fibres skin with an aqueous solution, said aqueous solution bearing a pH in the range of 5.0 to
9.), wherein said aqueous solution consisting of: 2.0 gm to 5.0gm of a carbon source per 1
kg of hemp fibre; 1.0gm to 5.0 gm of a nitrogen source per l kg of hemp fibre; biomaterial
obtained from a bio-resource material; storing, for natural fermentation, at ambient
temperature, in order to separate its fibres, for a time period ranging from 3.0 to 10 days;
subsequently, washing the separated fibres with water having a pH in the range of 5.0 to 8.0;
and treating the washed and separated fibres with one or more cleaning agent for more
cleaner fibres.

Reference may be made to a patent no:IN3226DEN2012A by Barton Nelson Ret., al with the
title Phospholipases, nucleic acids encoding them and methods for making and using them".
In alternative embodiments, the invention provides phosphatidylinositol-specific
phospholipase C(PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind
specifically to them, and methods for making and using them. Industrial methods and
products comprising use of these phospholipases are also provided. In certain embodiments,
nrovided herein are methods for hydration of
lipid matrix. The methods enable
migration
non-hydratable
of NHPs to an phospholipids (NHPs) within a
allowing the NHPs to be reacted and/or removed from the oil-water interface thereby
provided is a method for removing NHPs, hydratable lipids. In-certain embodiments,
phospholipids, and lecithins from
vegetable oils toproduce a degummed oil or fat product that can be used for food production
and/or non-food applications. In certain
hydration of NHPs followed by enzymatic embodiments, provided herein are methods for
treatment and removal of various phospholipids
and lecithins. The methods provided herein can be
practiced on either crude or water
degummed oils.
ABSTRACT

The invention discloses,degumming is the process of removing the sericin or gum from silk
varn. Removing the gum improves the sheen, color, hand and texture of the silk. Enzymes are
biocatalysts are being used to selectively target fibre accompanying impurities such as pectin,
hemicellulose, lignin, cellulose, proteins, fats, starch, greasy substance and oil during fibre
share in textile
recovery. Plant based fibres include jute, hemp, and ramie have a great market
of the hemicellulosic
industries. Textile processing of natural fibre necessitates the removal
effectiveness of pectinase and
substance. The objective of this research is to explore the
enzymes in degumming textile fibres, particularly natural fibres like silk and flax.
xylanase sustainable
various industries as effective and
These enzymes have shown promise in
In this study. pectinase and xylanase
alternatives to conventional chemical-based processes.optimized for degumming applications.
sources and
enzymes were extracted from microbial temperature, pH. and treatment duration were
as enzyme concentration,
Parameters such on
achieve maximum degumming efficiency while minimizing adverse effects
optimized to demonstrate that pectinase and xylanase
enzymes
study
the textile fibres. The results of thenon-cellulosic impurities from natural fibres
without
effectively remove gum and processes,
strength and quality. Compared to chemical degumming
compromising fibre consumption,
degumming offers several advantages, including reduced water significantly
enzymatic resulting in a
energy requirements, and the absence of toxic chemicals, process promotes
lower Furthermore, the enzymatic degumming
footprint.
reduced environmental
resources and supports the concept of circular economy by
the utilization of renewable degumming enzymes. The enzymes can be
regenerated.
and reuse of
enabling the recovery
and optimizing the overall process efficiency. This
generation eco-friendly
thereby reducing waste pectinase and xylanase enzymes as
research highlights the potential of degumming
industry. Implementing enzymatic minimizing
alternatives for degumming in the textile manufacturers by
sustainability goals of textile
processes ca1 contribute to the efficiency, and ensuring the production
of high
environmental impact, improving resource
quality textiles.
Pectinase, Xylanase.
Keywords: Hemicellulosic, Degumming,

TVENNOp's

NJ/23/06/2023
.Nao2/ob)2013
Rk.A |23 lo6/ro2}
CLAIMS

1Eco-friendly degumming using pectinase and xylanase improves sustainability in textile

industries.

2. According to claim 1, pectinase and xylanase treatments reduce chemical usage in textile
degumming processes.
3.According to claim 2, using pectinase and xylanase improves fiber quality and maintains
natural characteristics.

4.According to claim 1, enzyme-based degumming saves water and promotes water

conservation in textile production.
compliance
5. According to claim 2, adoption of pectinase and xylanase treatments ensures
with environmental regulations in textile industries.
reduces overallproduction
6. Cost-effective degumming solution with pectinase and xylanase
Costs.

and xylanase minimizes carbon emissions in

7. Energy-efficient degumming using pectinase
textile manufacturing.

APPKA's SbIN
Noll.G23/06 | 2023

RK-At/ 231o6/2023