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Laboratory Instructions For Fermentation Experiment

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12 views

Laboratory Instructions For Fermentation Experiment

Uploaded by

rinichi.asus
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Laboratory Instructions for Fermentation Experiment

Objective:

To investigate the process of fermentation using yeast and measure the production of carbon
dioxide gas.

Materials Needed:

1. Yeast suspension
2. Glucose solution (5% w/v)
3. Test tubes (5-10)
4. Rubber stoppers with delivery tubes
5. Small beaker or graduated cylinder
6. Water bath or incubator set at 37°C
7. Gas collecting apparatus (e.g., inverted graduated cylinder in water)

Procedure:

Preparation:

1. Labeling: Label each test tube with a marker to distinguish between different setups or
time points.
2. Yeast Suspension: Prepare a yeast suspension by mixing yeast with water or saline to
form a smooth paste. Use this suspension for all test tubes.

Experiment Setup:

3. Tube Setup:
o Fill each test tube about halfway with the glucose solution.
o Add yeast suspension to each test tube, ensuring consistent amounts across all
tubes (e.g., 1 ml).
o Mix gently to ensure yeast is evenly distributed.
4. Gas Collection Setup:
o Prepare the gas collection apparatus by filling a graduated cylinder with water and
inverting it into a larger container of water.
o Ensure the delivery tube from each test tube is securely inserted into the water-
filled graduated cylinder without trapping air.
5. Incubation:
o Place all test tubes in a water bath or incubator set at 37°C to maintain a constant
temperature.
o Allow the fermentation process to proceed for a specified time period (e.g., 30
minutes to 1 hour).

Data Collection:
6. Gas Measurement:
o During incubation, observe and measure the amount of gas collected in the
graduated cylinder at regular intervals (e.g., every 10 minutes).
o Record the volume of gas collected (in milliliters) for each test tube at each time
point.
7. Control Setup:
o As a control, prepare a test tube with glucose solution but no yeast. This will help
differentiate between gas production due to fermentation and any atmospheric
contamination.

Analysis:

8. Graphing Data:
o Plot a graph of volume of gas produced (y-axis) against time (x-axis) for each test
tube.
o Analyze the rate of gas production and compare between different setups (e.g.,
different concentrations of glucose, different amounts of yeast).
9. Interpretation:
o Discuss the results in terms of the fermentation process: how yeast metabolizes
glucose to produce carbon dioxide and alcohol.
o Consider factors influencing fermentation rate such as temperature, substrate
concentration, and yeast activity.

Safety Precautions:

 Handle yeast and glucose solutions carefully to avoid contamination.


 Ensure all glassware and equipment are securely assembled to prevent leaks or spills.
 Wear appropriate personal protective equipment (e.g., lab coat, gloves) as necessary.

Conclusion:

Summarize the findings of the experiment and relate them to the process of fermentation.
Discuss any limitations or sources of error encountered during the experiment.

References:

 Campbell, N. A., & Reece, J. B. (2005). Biology (7th ed.). Benjamin Cummings.
 Taiz, L., & Zeiger, E. (2010). Plant Physiology (5th ed.). Sinauer Associates.

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