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Activity 5e - Extraction

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ariesjay.reyes
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10 views

Activity 5e - Extraction

Uploaded by

ariesjay.reyes
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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ACTIVITY 3.

SEPARATION AND PURIFICATION OF SUBSTANCES

EXTRACTION
(Chlorophyll Extraction)

A frequently used method of withdrawing or separating a substance from a mixture is


extraction by the use of solvents. In applying this method, the mixture is dissolved in one of two
miscible solvents and then shaken with the other to distribute the components between them. The
amount of components in each of the 2 solvents will be in direct proportion to their relative
solubility in each of them.

Extraction with water – immiscible solvent is useful for isolation of natural products that
occur in animal and plant tissues having high water content.

Reagents and Materials:

Pechay leaves/ Alugbati seeds

25 ml 95% Ethyl alcohol


Procedure:

1. Remove white stems of pechay leaves and wash the green portion thoroughly to remove
dirt and soil adhering to it. Air – dry the leaves.
2. Slice green leaves finely and weigh 10 grams of it.
3. Immerse them in 25 ml of 95% ethyl alcohol. Place it in the beaker and let it stand for 1
hour in a dark, cool place.
4. Decant the liquid, and then filter.
5. Collect the green extract in an evaporating dish.

Questions:

1. What is the principle involved in extraction?


2. What are indications that the desired substances have been extracted?
3. What is the role of ethyl alcohol in the extraction of chlorophyll?
4. What is “salting – out”? When is it used in the extraction process?

Name Date
Group no.

Activity 3.5
SEPARATION AND PURIFICATION OF SUBSTANCES
Extraction
(Chlorophyll extraction)
I. Results and Observation
A. Extraction of Chlorophyll
Describe the color of the Extract
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Other descriptions about the extract
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