Bio-Ink for 3D Bioprinting

Sameeksha Dubey
INTERN

Education Department of SIFS

Abstract
A new technology called bioprinting can be used to generate functioning tissue constructions
to replace damaged or unhealthy tissues. This is a relatively new method that offers highly
reproducible and accurate autonomous oversight over the constructed structures, potentially
opening the door to high-throughput production. Tissue buildings are created by the
bioprinting approach utilizing a solution of a biomaterial or an amalgamation of numerous
biological materials in the hydrogel form, known as the bioink, which often encapsulates the
appropriate cell types.
To create the final shape, structure, and architecture of the intended construct, this bioink can
be cross-linked or stabilized during or just after bioprinting. Bioinks can be fabricated solely
from natural or synthetic biomaterials, or as hybrid materials, from a blend of the two. In rare
instances, cell aggregates can also be used as a bioink for bioprinting procedures without the
need for any extra biomaterials. In order to guarantee optimum functionality of the bio
printed tissues and organs, an ideal bioink should have the appropriate mechanical,
rheological, and biological properties of the target tissues.

Introduction
In 3D bioprinting, living cells and biomaterials are deposited layer by layer to produce
structures that resemble tissues. Using bio-ink, cells and biomaterials can be delivered in a
controlled way while maintaining the native tissue architecture. The intricacy and
functionality of bio printed constructions have been greatly increased by the development of
bio-ink formulations, making bioprinting an effective tool for tissue regeneration and
personalized treatment.
The creation of bio-inks has greatly increased the 3D bioprinting process's viability and
adaptability, allowing scientists and medical professionals to create patient-specific tissues
and organs with complex architectures and customized functionality. These biocompatible
materials, which include synthetic and natural polymers like polyethylene glycol and
polycaprolactone, as well as alginate, collagen, and gelatin, are used to formulate these bio-
inks. These materials can be adjusted to meet the precise mechanical, biological, and
degradation properties required for different tissue engineering applications.

Formulation of Bioink

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a. Polymers or Biomaterials > b. PEG Cross-Linkers (PEGX) > c. Cells are introduced >
d. Cells are Lightly Cross-linked with Gel > e. Heavy Cross-Linking > f. Versality of
chemicals to increase quality bioinks > g. Post printing and secondary Cross-linking.
 a. Polymer or polymer blends in multifunctional, branching (4-arm PEG amine), or
linear (gelatin) forms. PEGX's SVA groups, methacrylate groups, and amines.

b. PEGX can have different chain lengths and can be either multiarmed or linear.

c. The bioink can be created by

d. Combining polymers and PEGX with cells.

e. As an alternative, after printing, additional cross-linking can be done to boost

mechanical strength.

f. Polymers with different functional groups can be cross-linked by modifying the

reactive groups of PEGX. Alkynes, which are cross-linkable with azide PEGX and
thiols, which are cross-linkable with maleimide PEGX.

g. Phases involved in the PEGX bioink printing process: PEGX, either with or without
cells, was combined with the polymer solution and put into the printing cartridge.
Once gel formation and reliable mechanical properties were obtained, multilayered
conceptualizations could be 3D manufactured with gels.

Types of Bio-Printing:

In extrusion-based bio-printing, beads or continuous filaments of

E x tru sio n -B ase d B io -P rin tin g material are deposited by pushing bio-ink via a syringe or nozzle.

In k jet-B a sed B io -P rin tin g Similar to conventional inkjet printers, inkjet-based bio-printing
employs bio-inks which are made of live cells instead of ink.

L a ser-B a sed B io -P rin tin g In laser-based bio-printing, biomolecules and cells were specifically
placed onto a substrate by means of lasers.

Future Prospects of 3D Printing

Using living cells and biomaterials, bioprinting is a potential technique to manufacture 3D
tissue constructions with precisely defined forms and geometries. Biomaterials (such
hydrogels), cells, cell aggregates, or their combinations are commonly used as bio-inks,
which are a crucial part of bioprinting.

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Many synthetic and natural polymers, including PCL, PEG, and Pluronic, as well as gelatin
and alginate, have been used as bioinks. Despite the fact that there are many attempts being
made to advance the technology of bioprinting, there hasn't been much progress made in
developing suitable bioinks that fulfill the mechanical, rheological, and biological
requirements of bioprinting. Therefore, two current hot topics of interest are the creation of
innovative bioink formulations and new bioink materials.
Furthermore, additional work has to be done on developing standards and models for
comparing and assessing the characteristics of various bioink materials. In order to achieve
this goal and standardize the applications of bioinks and bioprinting procedures, new metrics
must be created. As previously indicated, a groundbreaking study has developed a technique
to assess the shape integrity of bioinks by measuring the filament collapse on overhanging
structures and the filament fusing of parallel-bio printed strands using an extrusion bioprinter.

To thoroughly examine the printability and behaviours of the bioinks before experimental
modifications, it is also interesting to design new computational models. This review has
included comprehensive information regarding existing bioinks in light of the aforementioned
topics, and it is believed that this will be helpful to a wide readership in the bioprinting and
tissue engineering fields.

References

 P. Bajaj, R. M. Schweller, A. Khademhosseini, J. L. West and R. Bashir, Annu. Rev.

Biomed. Eng., 2014

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  P. Selcan Gungor-Ozkerim , Ilyas Inci, Yu Shrike Zhang, Ali Khademhosseini, and
Mehmet Remzi Dokmeci, RSC, 2018
 A. L. Rutz, K. E. Hyland, A. E. Jakus, W. R. Burghardt and R. N. Shah, Adv. Mater.,
2015
 A. Ribeiro, M. M. Blokzijl, R. Levato, C. W. Visser, M. Castilho, W. E. Hennink, T.
Vermonden and J. Malda, Biofabrication, 2017
 Murphy SV, Atala A. 3D bioprinting of tissues and organs. Nat Biotechnol. 2014
 Chimene D, Lennox KK, Kaunas RR, Gaharwar AK. Advanced bioinks for 3D
printing: a materials science perspective. Ann Biomed Eng. 2016
 Gungor-Ozkerim PS, Inci I, Zhang YS, Khademhosseini A, Dokmeci MR. Bioinks for
3D bioprinting: an overview. Biomater Sci. 2018
 Ozbolat IT, Hospodiuk M. Current advances and future perspectives in extrusion-
based bioprinting. Biomaterials. 2016

Ghosh, S., & Yi, H.-G. (2022). A review on Bioinks and their application in plant
bioprinting. International Journal of Bioprinting, 8(4), 612.
https://doi.org/10.18063/ijb.v8i4.612

Ghosh, Susmita, and Hee-Gyeong Yi. “A review on Bioinks and their application in plant
bioprinting.” International Journal of Bioprinting, vol. 8, no. 4, 2 Sept. 2022, p. 612,
https://doi.org/10.18063/ijb.v8i4.612.

Chae, Suhun, and Dong-Woo Cho. “Biomaterial-based 3D bioprinting strategy for

Orthopedic Tissue Engineering.” Acta Biomaterialia, vol. 156, Jan. 2023, pp. 4–20,
https://doi.org/10.1016/j.actbio.2022.08.004.

 Chae S, Cho DW. Biomaterial-based 3D bioprinting strategy for orthopedic tissue

engineering. Acta Biomater. 2023 Jan