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Bioscience Syllabus

The document outlines the objectives, experiments, and outcomes of a biochemistry laboratory course. The course aims to provide practice in estimating biomolecules, separating macromolecules, using microscopes, and performing chemical and histopathological examinations. It includes experiments on solutions, spectroscopy, identifying blood tubes, separating proteins, staining techniques, and examining histopathology slides.

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26 views

Bioscience Syllabus

The document outlines the objectives, experiments, and outcomes of a biochemistry laboratory course. The course aims to provide practice in estimating biomolecules, separating macromolecules, using microscopes, and performing chemical and histopathological examinations. It includes experiments on solutions, spectroscopy, identifying blood tubes, separating proteins, staining techniques, and examining histopathology slides.

Uploaded by

daitdesignerclub
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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BM3271 BIOSCIENCES LABORATORY LT PC

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COURSE OBJECTIVES:
To provide practice on:
 Estimation and quantification of biomolecules.
 Separation of macromolecules.
 Use Compound microscope
 Practice on chemical examinations, Histopathological examinations etc

LIST OF EXPERIMENTS:
1. Preparation of solutions: 1) percentage solutions, 2) molar solutions, 3) normal solutions
2. Standardization of pH meter, preparation of buffers, emulsions.
3. Spectroscopy: Determination of absorption maxima (λmax) of a given solution
4. General tests for carbohydrates, proteins and lipids.
5. Identification of Blood Collection Tubes and Phlebotomy equipment
6. Preparation of serum and plasma from blood
7. Estimation of Haemoglobin and blood glucose
8. Estimation of creatinine, urea and Uric acid
9. Separation of proteins by SDS electrophoresis(Demo) and amino acids by thin layer
chromatography (Demo).
10. Urine physical and chemical examination (protein, reducing substances, ketones, bilirubin
and blood)
11. Basic staining – Hematoxylin and eosin staining.
12. Special stains – cresyl fast Blue (CFV)- Trichrome – oil red O – PAS
13. Types of Staining : Simple stain, Gram stain
14. Study of parts of compound microscope
15. Study of Histopathological slides of benign and malignant tumours.
16. Study of Haematology slides of anemia and leukemia.
TOTAL: 60 PERIODS
COURSE OUTCOMES:
Upon completion of the course, students will be able to: CO1:Understand
the Biochemistry laboratory functional components CO2:Have a sound
knowledge of qualitative test of different biomolecules.
CO3:Understand the basics knowledge of Biochemical parameter and their interpretation in Blood
sample.
CO4:Have a sound knowledge of separation technology of proteins and amino acids.
CO5:Student can perform practical experiments on staining Processes.

TEXT BOOK:
1. Ramnik Sood, Textbook of Medical Laboratory Technology, 6thEdition, Jaypee Brothers
Medical Publishers, 2009

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