An Introduction to Scientific Investigation1

Laboratory Objectives
1. Identify questions that can be answered through scientific investigation and explain
what characterizes a good question.
2. Define hypothesis and explain what characterizes a good scientific hypothesis.
3. Identify and describe the elements of a scientific experiment.
4. Organize and present data in tables and graphs.
5. Analyze and discuss results.
6. Design a scientific experiment.

Introduction:
Biology is the study of the phenomena of life. Biological scientists--researchers,
teachers, or students--observe living systems and organisms and ask questions about such
observations. Scientific investigation is a way of answering those questions. It assumes that
biological systems are understandable and can be explained by fundamental rules or laws.
Scientific investigations follow some general guidelines that are referred to as the scientific
method. Scientists may not always follow the guidelines in a strict fashion, but each of the
elements of the process should be present. Science is a creative human endeavor that
involves asking questions, making observations, developing hypotheses, and testing those
hypotheses. Scientists closely scrutinize investigations in their field. Each scientist must
present his or her work at scientific meetings or in professional publications to provide
evidence from observations and experiments that support their explanation of biological
phenomena.
In this lab, you will not only review the process that scientists use to ask and answer
questions about the living world, but you will develop the skills to conduct and critique
scientific investigations. Science is a collaborative effort in which scientists work together and
share their results. Like scientists, you will work in research teams in this laboratory and
others, collaborating as you ask questions and solve problems. Throughout the laboratory
experience, you will be investigating biology using the methodology of scientists, asking
questions, proposing explanations, designing experiments, predicting results, collecting and
analyzing data, and interpreting your results in light of your hypotheses.
While the scientific method may vary from one study to the next, or from one discipline
to another, its basic components are shown below:

Observation

Asking Questions

Formulating Hypotheses

Predictions

Experimentation/Testing Hypotheses

Conclusions

1 Portions based on materials by Dr. Andrew Marry, Department of Biosciences, Minnesota State University Moorhead
I. Observation; Asking Questions:
The questions that are answered by scientific investigation are based on observations,
information gained through previous research, or a combination of both. Just because a
question can be answered, however, does not mean that it can be answered scientifically.

Activity I.
Discuss the questions at the end of section IV with your lab team and decide which of them
can be answered scientifically. State “yes” or “no” and give your reasons.

II. Developing Hypotheses:

As questions are asked, scientists attempt to answer them by proposing possible
explanations. A hypothesis is a tentative explanation for what we observe or a proposed
answer to a question. Consider question 2 “Why is grass green?”. A possible hypothesis
based on this question might be "Chlorophyll located in leaf cells causes grass to be green."
The hypothesis has suggested a possible explanation for the observed green grass.
A hypothesis is usable only if it can be proven false. This is called being falsifiable.
The nature of science is such that we can prove a hypothesis false by presenting evidence
from an investigation that does not support it. In our example, if all chlorophyll is removed
from the leaves of a test plant and the plant remains green, then the hypothesis has been
proven false. We cannot, however, prove that a hypothesis is true. We can only support the
hypothesis with evidence from our investigation. In our example, if chlorophyll is extracted
from the test plant and the plant now appears white, then the hypothesis has not been proven
true, but has been supported by the evidence. New evidence from additional experiments
might falsify this hypothesis at a later date.
Scientific knowledge is an accumulation of evidence in support of hypotheses; it is not
to be regarded as absolute truth. Hypotheses are accepted only on a trial basis. Future
investigations may falsify any hypotheses. However, this does not mean that scientific
knowledge is flimsy and unreliable. Much of the information in your textbook, for example, is
based on many experiments carried out by numerous scientists over a period of years. On the
other hand, current scientific studies you read about in the newspaper--for example,
investigations of the effects of caffeine--are much more preliminary and therefore more
tentative. You may even read about studies with contradictory results. These are based on
hypotheses still under investigation.
There are limits to the ability of science to answer questions. Science is only one of
many ways of knowing about the world in which we live. Recall that scientific inquiries are
limited to hypotheses that are testable and potentially falsifiable.

Activity II.
Working in your groups, write hypotheses in the space provided for the questions that your
group felt could be answered by scientific inquiry.

III. The Elements of an Experiment:

Once a problem has been defined and one or more hypotheses have been proposed,
the scientist turns his or her attention to testing the hypotheses. An experiment involves
defining variables, outlining a procedure, and determining what control treatment to be used
as the experiment is performed. Once the experiment is defined, the investigator predicts the
outcome of the experiment based on the hypotheses. A crucial step in designing an
experiment is identifying the variables involved. There are three categories of variables:
dependent, independent, and constants.
The Dependent Variable
The dependent variable is what the investigator measures (counts, records). It is what
the investigator thinks will be affected during the experiment. For example, the investigator
may want to study soybean growth. There are several measurable characteristics of soybean
growth such as height, weight and soybean production. For any experiment, there may be a
number of possible dependent variables. The investigator must choose the one or ones that
he or she thinks is most important. More than one dependent variable may be chosen.

Independent Variable
The independent variable is what the investigator varies during the experiment. It is
what the investigator thinks will affect the dependent variable. Unlike the dependent variable,
the investigator must choose only one independent variable to investigate. For example, if the
scientist wants to investigate the effect that the amount of fertilizer has on soybean growth,
the scientist will use different amounts of fertilizer. The scientist can measure, as many
dependent variables as he or she thinks are important in indicating soybean growth.
Remember, there may be several dependent variables, but there may be only one
independent variable.

Constant(s)
Since there can be only one independent variable, all independent variables other than the
one being studied must be held constant so that they do not affect the outcome of the
experiment. For example, consider an experiment studying fertilizer effects on soybean
growth, where the scientist has chosen the amount of fertilizer as the independent variable.
The scientist must be sure that there are no differences in the type of fertilizer used. There
are many other variables that have to be held constant in this experiment. All plants should
be the same and have the same light exposure, soil type, amount of water and temperature.
It is impossible to hold everything constant, but scientists try to control as much as they can.

Activity III.
Working in your groups, identify the independent and dependent variables, and list the
parameters that may have to be held constant for the questions that your group felt could be
answered by scientific inquiry.

IV. Choosing or Designing the Procedure:

After formulating a hypothesis and selecting the variables, the investigator must find a
method that may be used to measure the dependent variable. Procedures are developed by
reading articles published by other scientists, by talking to other scientists who are
knowledgeable in the field, and by one's own ideas. In a biology class, procedures are usually
described in the lab manual or by your instructor. When performing investigations, the
scientist usually outlines the procedures in a notebook, recording any modifications as the
experiments are performed and procedures are revised. The process of outlining the
procedure includes deciding on the number of replications, and determining the control
treatment.

Replication
Another essential aspect of experimental design or procedure is replication. This
means that the scientist repeats the experiment numerous times using exactly the same
conditions to determine if the results are consistent. Being able to replicate a result increases
our confidence in it. However, we should not expect to get exactly the same answer each time
because a certain amount of variation is normal in biological systems. Replicating the
experiment enables us to see how much variation there is and to obtain an average result
from different trials.

Control Treatment
We have already discussed constant variables, factors that are kept equal in all
treatments so that any effect of the dependent variable can be attributed to the independent
variable. It is also necessary to include control treatments in an experiment. A control is a
treatment in which the independent variable is either eliminated or set at a standard value.
The results from the control treatment are what the experimental data are compared to once
the data has been collected.

Activity IV.
Working in your groups, identify an appropriate control treatment for the questions that your
group felt could be answered by scientific inquiry.

QUESTIONS
1. Is communism evil?
Activity I:

Activity II:

Activity III:

Activity IV:

2. Why is grass green?

Activity I:

Activity II:

Activity III:

Activity IV:

3. Was the malignant tumor found in the lungs of a 70-year-old man caused by his 45-year
habit of smoking cigarettes?
Activity I:

Activity II:

Activity III:

Activity IV:

4. Does watching television as a child lower your I.Q.?

Activity I:
 Activity II:

Activity III:

Activity IV:

5. Are children more assertive in 1st grade if they spend a year in nursery school at age 4?
Activity I:

Activity II:

Activity III:

Activity IV:

V. Making Predictions:
The process of designing the experiment is closely linked with making predictions
about results of the experiments being designed. The scientist applies his or her present
knowledge to predict the effect of the independent variable on the dependent variable. The
prediction is a statement of the expected results of the experiment based on the hypothesis.
The prediction is often an "if/then" statement: "If the hypothesis is true, then the results of the
experiment will be..." In the fertilizer experiment, the hypothesis might be: "Applying greater
concentrations of fertilizer to plants increases plant growth." One possible prediction may be
that as the amount of fertilizer is increased, the growth of the soybeans will increase in a
linear fashion.
If predictions are confirmed, the scientist has supported the hypothesis. If the
predictions are not supported, the hypothesis is falsified. Either way, the scientist has
increased knowledge of the process being studied. Many times the falsification of a
hypothesis can provide more information than confirmation since the ideas and data must be
critically evaluated in light of new information. For example, according to the above prediction,
you would expect the following graph:

1.25

1
Plant Growth (mg)

0.75

0.5

0.25

0
0 1 2 3 4 5 6 7 8 9

Amount of Fertilizer (mg)

However, the actual data may produce this graph:

 0.4

0.35

0.3

Plant Growth (mg)

0.25

0.2

0.15

0.1

0.05

0
0 1 2 3 4 5 6 7 8 9

Amount of Fertilizer (mg)

The scientist has learned that the prediction of “greater applications of fertilizer cause
increased growth” is true only to a point. The scientist may now wish to identify this point
specifically by finding out what would be the optimum amount of fertilizer to apply. The
scientist may also want to extend the research in a new direction and discover why the
highest fertilizer applications actually decrease the weight of the plants.

The necessary components of the scientific process discussed to this point are:
1. Using observations to ask good questions.
2. Proposing explanations for observed phenomena in the form of hypotheses.
3. Determining the components of an experiment.
4. Making predictions based on possible experiments.

Having made predictions about the results of an experiment, the next step in the scientific
process is to perform the experiment. As the investigation takes place, observations are
made, results are recorded and the hypotheses is either supported or falsified.

VI. Reading a scientific study:

Read the article provided by your instructor. After reading the study, answer the following:

1. What is the hypotheses?

2. What is the dependent variable?

3. What is the independent variable?

4. What were the constant variables?

5. What was the control treatment?

6. How many replicates were conducted? Why?

VII. Designing an Experiment:
In this exercise will practice investigating a question using what you have learned thus
far about the scientific process.

Materials
Clock with a second hand/timer

Introduction
Cardiovascular fitness can be determined by measuring a person's heart rate and
respiration rate before and after a given time of aerobic exercise. A person who is more fit
may have a relatively slower pulse and a lower respiratory rate after exercise; along with this,
his or her pulse should return to normal more quickly than a person who is less fit. Your
assignment is to investigate the impact of some well-defined, measurable, controllable,
independent variable on cardiovascular fitness.

The sit/stand experiment

The subject should perform a trial of one or two repetitions before conducting the actual test
to clarify form.
-Sit on chair with feet shoulder width apart close to chair.
-Keep arms crossed in front of you.
-Stand up without pushing off with arms at a rate of 30 sit/stands per minute.
-Repeat until 60 seconds expires.

Test administrator provides test instructions to subject, keeps time, monitors sit/stand rate
and announces when time has been completely.

Procedure for measuring pulse

-The subject should be sitting quietly while the pulse is counted.
-Use three fingers to find the pulse in the radial artery, in the wrist above thumb.
-Count the number of beats per 15 seconds and multiply by 4 to determine beats per minute.

Performing the experiment

The class will split into two groups. Each member of the group is a possible test
subject. All students in the group will be the investigators.
In your research teams, take about 5 minutes to discuss several specific questions that
you can ask and independent variables related to the broad topic of cardiovascular fitness.
For example, one of your questions might be: "Does cigarette smoking have an effect on
cardiovascular fitness?" Make an effort to choose questions that will allow divide the class into
two groups for easy comparison.
In your groups, design an experiment. Record the components of your experiment by
completing the information on your report sheet. Propose your experimental procedure to
your laboratory instructor before beginning your experiment. You should have your data table
ready to collect data before you begin your experiment. Remember to identify the following:

Hypotheses:

Dependent variable(s):
Independent variable:

Controlled variables:

Control treatment:

Predictions
Predict the results of the experiment based on your hypothesis (If/Then).

Results
Create a table to collect your data. You may also want to graph your data at the end of the
experiment:
VII. Interpreting Results:
The last component of a scientific investigation is to interpret the results and discuss their
implications in light of the hypothesis and its supporting literature. The investigator studies the
tables and graphs and determines if the hypothesis has been supported or falsified. If the
hypothesis has been falsified, the investigator must suggest alternate hypotheses for testing.
If the hypothesis has been supported, the investigator may suggest additional experiments to
strengthen the hypothesis, using the same or alternate methods.

1. Using your tables and graphs, analyze your results and discuss your conclusions.
2. Critique your experiment. What weakness do you see in the experiment?

Weakness in Experiment:

Suggest improvements:

3. Write a summary statement for your experiment. Use your results to support or falsify your
hypothesis.

4. Be prepared to present your conclusions to the class.

5. Suggest additional and modified hypotheses that might be tested. Briefly describe your next
experiment.