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1.0 OBJECTIVE:
To lay down the procedure for operation and calibration of polarimeter Jasco P- 2000.

2.0 SCOPE:
This SOP is applicable for operation and calibration of polarimeter Jasco P-2000 in Quality Control
Department

3.0 RESPONSIBILITY:
Preparation & Follow-ups : Quality Control Chemists / Officers / Executives / Sr. Executives
Checking & Implementation : Head of the department (HOD) / Designee
Approvals : Head Quality Assurance
Authorization : Plant Head

4.0 ACCOUNTABILITY:
Head Quality Control and Quality Assurance.

5.0 ABBREVIATIONS:
SOP : Standard Operating Procedure.
No. : Number
CCF : Change Control format
HH : Higgs healthcare
QC : Quality Control
QA : Quality Assurance

6.0 PRECAUTIONS:
6.1 Handle polarimeter instrument and tube carefully.
6.2 Do not keep polarimeter tube without cleaning after use. It should be clean after each use.
6.3 Keep switch off the instrument when should be clear.

7.0 MATERIALS AND EQUIPMENTS REQUIRED:

7.1 MATERIALS: Sucrose and purified water.
7.2 EQUIPMENTS: Polarimeter, computer & printer.

8.0 PROCEDURE :
8.1 Operation:
8.1.1 Switch on computer system first and then switch on the instrument by clicking power
button present left side of instrument.
8.1.2 Double Click on icon of polarimeter present on Desktop.

8.1.3 Instrument connects with computer system shows idle in condition.

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8.1.4 Click on standard measurement icon below instrument.

8.1.5 Light source take time to warming and show time remain for warming as Shown in
picture below . Do not click on skip button.

8.1.6 After click on Standard measurement window will open as in shown in Picture mention
below.

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Tool Bar

Menu Bar

Zero clear button

Light Source Save button

Data list Sample Aperture

Status Bar

8.1.7 Click on measure button on toolbar scroll down below click on parameter, parameter to
be set open as shown in picture below.

8.1.8 Click on general button on screen select and set the value for different parameter as
mention below.
8.1.8.1 Select Light source as Sodium Na,
8.1.8.2 Wavelength 589,
8.1.8.3 Cycle time 5sec,
8.1.8.4 Cycle interval 5sec.

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8.1.8.5 Put desire temperature for analysis,

8.1.8.6 Select Analysis mode Optical Rotation, Specific optical rotation or
concentration.
8.1.8.7 Put path length100mm.
8.1.8.8 Insert value of factor 1 if not mention in test procedure, if mention then put
the same.
8.1.8.9 Putting all parameter press ok button.
8.1.8.10 Click on Control button,Select sample compartment 8.0mm And light source
Auto and press ok.

8.2 Click on Accessory button on set parameter window.

8.2.1 Put desire temperature.

8.2.2 Select control sensor Holder.
8.2.3 In start condition select start measurement immediately.
8.2.4 Press ok button.

8.3 Click on Expansion button on set parameter window. Measure end time 300 second and
Sampling interval 5 second and press ok.

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8.4 Put desire information Sample Name, Operator Name and Comment and press ok button.

8.5 Click on Data button in parameter window.

8.5.1 Click on Auto save.
8.5.2 Select the desire folder to save data.
8.5.3 Press the Ok button.

8.6 Fill the Sample tube with diluent perform zero clear by clicking.
8.6.1 After Auto zero perform click on Measure button on tool bar scroll down and select Blank
as shown in Figure

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8.6.2 On click on Blank measurement tools open with a message “Click on [Measure
Button] after preparing blank for measurement” as shown in figure.

8.6.3 After Blank measurement click on Measure button on tool bar scroll down and Select
sample window open as shown in fig.

8.6.4 Insert sample name and Batch No. as comment and click on measure.
8.6.5 Save the result.
8.6.6 After analysis print the result by click Print.
8.6.7 Record the analysis in Instrument/Equipment Usage Log Book (HH-III\QC\008\F-01\00)
8.7 CALIBRATION:
8.7.1 Prepare the standard solution of sucrose (50% stock) by dissolving 50 gm of Sucrose
(previously dried 1050C for 2 hrs) to 100 ml with purified water.
8.7.2 Further dilute 5ml, 10ml, 15ml & 20ml of stock solution to 25ml separately to get 10%,
20%, 30%, & 40% Standard sucrose solution.
8.7.3 Measure the optical rotation in a 1dm tube as applicable at 20°C ± 0.5°C using purified
water as blank.
8.7.4 Reading should be comparable with values given below.

Sr. No. Concentration (w/v) Angle of rotation Tolerance

for 2dm cell
1 10% 13.330 13.08° to 13.58°

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2 20% 26.610 26.36° to 26.86°

3 30% 39.860 39.61° to 40.11°
4 40% 53.060 52.81° to 53.31°

5 50% 65.980 65.98° to 66.48°

8.7.5 For 1dm measurement, multiply the reading with 2, to get the corresponding reading for
2dm cell.
8.7.6 Record the results in the format attached Annexure – I.
8.7.7 Calibration Frequency: Half yearly.

9.0 ENCLOSURES:
Annexure -I Polarimeter calibration record Format No.: HH-III/QC/011/F-01/00

10.0 DISTRIBUTION:
10.1 The SOP shall be distributed to respective department by QA. The QA department shall
Authorize for the distribution of SOP and shall sign for “Issued by“
Follow the distribution of SOP as per following:
Master Copy - QA Department (In documentation cell)
Control Copy - User Department
10.2 The Revised SOP shall be distributed only after recall of superseded SOP.

11.0 Reference
User Manual
IP 2022 chapter
Sop for usage of Equipment /Instrument

12.0 HISTORY OF REVIEW:

PREVIOUS PREVIOUS CCF NO. (If any) REASON FOR REVIEW
EFFECTIVE DATE REVISION NO.
Nil Nil Nil New SOP.
10/01/2020 00 Nil Periodic review.

Annexure – I
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POLARIMETER CALIBRATION RECORD

Polarimeter I.D.: ……………. Make: ………………….

Balance I.D.: ……………… Make : …………………
Sucrose Batch No.: ……………… Make : …………………

Preparation of sucrose stock solution:

Weight accurately ……………... (50gm) (previously dried 1050C for 2 hrs) sucrose and dissolving in 100ml
volumetric flask to 100ml with purified water.
Solution preparation table:
Sr. No. Concentration (w/v) Taken solution from stock solution Dilute with water
1 10%
2 20%
3 30%
4 40%
5 50%

Observation Table:
Sr. Concentration (w/v) Observed Angle of rotation Tolerance
No. for 2dm cell
1 10% 13.08° to 13.58°
2 20% 26.36° to 26.86°
3 30% 39.61° to 40.11°
4 40% 52.81° to 53.31°
5 50% 65.98° to 66.48°

Remark: The polarimeter comply /does not comply with predefine calibration parameter.
Prepared By……………. Reviewed By…………….
Format No. HH/QC/062/F-02/01

END OF SOP

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