BSCMSC
BSCMSC
SEMESTER II
Content:
Unit 1:Elemental Composition of Biomolecules; Properties of Water, hydrogen bonding and its
biochemical properties; Concept of pH, pKa and buffers; Basic structure and function of Biological
Macromolecules: Amino acids, Nucleotides and Monosaccharides, fatty acids (building blocks)
Proteins, Nucleic Acids, Carbohydrates and Lipids (polymers)
Unit 2: Cellular organization, Cell theory, cell types: prokaryotes vs. eukaryotes, single cell to multi-
cellular organism. Cell structures, beginning with the cell envelope of bacteria, plant and animal cells,
cell membranes and their properties and structure of the cellmembrane.
Unit 3: Cytoskeletal components: actin, microtubules and microfilaments and motor proteins. Cell
organelles, Cell nucleus and chromatin structure.
Unit 4: Fundamental processes: DNA replication, mitosis and meiosis, RNA, transcription and
translation.
Laboratory Course
Content: This practical course will cover biochemical, genetic and molecular basis of life. Practicals
include: Glucose estimation; Lipid estimation; Amino acid Paper chromatography; Protein estimation;
Enzyme assay and Kinetics; Human genetic traits and blood grouping; DNA isolation; DNA
estimation; Transformation; Plasmid isolation; Agarose gel electrophoresis; Restriction digestion and
Ligation; PCR demonstration; ATC PTC demonstration; Animal handling, inoculation, dissection.
Recommended Readings:
1. Biochemistry: D. Voet, and J.G. Voet (2010), 4th edition,Wiley
2. Harper's Illustrated Biochemistry: R. Murray, V. Rodwell, D. Bender, K.M. Botham, P.A.
Weil and P.J. Kennelly (2009) 28th edition, McGrawHill-Medical
3. Biology: N. Campbell and J. Reece (2005) 7th edition, Pearson, Benjamin,Cummings
4. Biology: P.H. Raven, G.B. Johnson, J.B. Losos and S.R. Singer (2005) 7th edition, McGraw
Hill
5. Molecular Biology of the Cell: B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts and P.
Walter (2007) 5th edition, GarlandScience.
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