Agilent BioTek Synergy H1

Microplate Reader
Designed for flexibility and performance
Agilent BioTek Synergy H1
Microplate Reader

The Agilent BioTek Synergy H1 is a configurable multimode

microplate reader, with monochromator-based optics
for flexibility, filter-based optics for sensitivity, or both.
The proprietary Agilent BioTek Hybrid Technology offers
applications versatility and excellent performance in a modular
platform to expand as your laboratory’s needs change.

Ready for any assay

The modular design of the Synergy H1 allows you to start with what you
need now, and add detection modes, gas control, and dual-reagent injectors
as your laboratory’s workflows evolve.

Absorbance Fluorescence Glow Flash

intensity luminescence luminescence

Filtered Fluorescence Time-resolved TR-FRET

The Agilent BioTek Synergy H1
luminescence polarization fluorescence
shown with CO 2 /O 2 gas controller
and dual-reagent injector.

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" Agilent BioTek's Synergy H1 is an easy to use instrument that
allows flexibility of assays and analysis. The customer support
team and local rep have always been highly responsive to our
lab's needs."
– Kate Mueller,
University of MN

Hybrid plate reader—flexibility

and performance
With its proprietary combination of
monochromator and filter optics,
Synergy H1 is an advanced plate reader
that delivers both the flexibility and
performance you need for a wide range
of microplate assays in your lab.
Monochromators: Variable bandwidth,
absorbance, fluorescence, and
luminescence.
Filters: Fluorescence polarization,
time-resolved fluorescence, and filtered
luminescence.

Variable bandwidth for

sensitivity and specificity
Synergy H1 offers quad
monochromator optics with
variable bandwidth. The excitation
and emission bandwidths can be
set between 9 and 50 nm, in 1 nm
increments. Large-bandwidth settings
(1) provide increased sensitivity
and lower limits of detection. Small
bandwidths (2) provide increased
specificity when multiple signals
are present, reducing crosstalk and
enhancing assay performance.

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 "It is very effective equipment for all the assays using absorbance quantification methods. High
sensitivity and reproducibility to detect samples. This equipment is very easy to use and came with
a reasonable pricing and a good after-sales care."
– Lufen Chang
City of Hope National Medical Center and
Beckman Research Institute Department of
Cancer Genetics and Epigenetics

Automated Z-focus—best
performance with all plate types
Without automated Z-focus available
(1), performance at low volumes is
affected. With automated Z-focus (2),
reading height is precisely adjusted for
best performance in all plate types and
all volumes.

Extended dynamic range

Gain 3 Synergy H1 offers an extended
dynamic range, which allows detection
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Gain of signals across a 7 log measurement
Signal

range. Other systems can measure

1
Gain only small portions of the dynamic
range of Synergy H1 using preset
Extended dynamic range gains—this can cause reduced
sensitivity on the low end or saturated
Concentration signals on the high end of the assay
signal range.

Environmental controls for cell-

based assays
Temperature control to 70 °C,
CO2 /O2 control, and shaking create the
ideal environment for live cell assay
workflows. A consistent environment
leads to consistent data for long-term
kinetic assays.

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Agilent BioTek Synergy H1
Synergy H1 now offers continuously
variable bandwidth monochromators
for fluorescence excitation and
emission wavelength selection; the
fluorescence bandwidth can be
set between 9 and 50 nm, in 1 nm
increments, allowing users to fully
optimize reader settings to drive the
best assay performance compared to
fixed-bandwidth systems.

Dual-syringe injectors with

specialized tips
The robust precise dual-syringe design
(1) eliminates the need for regular
tubing replacement required by some
peristaltic pump injector designs.
Synergy H1 offers two tip types: the
straight tips (2) enable vigorous mixing
for rapid inject/read assays, and the
angled tip option (3) won’t disturb cell
layers for applications such as calcium
kinetics.

Microvolume analysis with the

Take3 microvolume plate
Enable microvolume analysis with
the Synergy H1, using theTake 3
microvolume plate. Measure up to 16
or 48 samples in one run and save
a lot of time, compared to single-
sample devices. The available Agilent
BioTek Take3 app is preprogrammed
for ssDNA, dsDNA, RNA, and protein
quantification in 2 μL samples.

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Applications
ELISA Luciferase reporter assays

ELISA methods with colorimetric, fluorescent, and luminescent substrates Luciferase-based reporter assays measure luminescent signal. This
are easily detected with Synergy H1. enables users to quantify the activity of factors that affect particular
signaling pathways.

Nucleic acid and protein quantification Microbial growth assays

Nucleic acid and protein quantification assays can be executed by Microbial growth assays including yeast and bacteria, for example, can be
spectrophotometric or fluorescent determination with Synergy H1, in measured by several methods, including turbidimetric measurements with
microplates or in microvolumes with the Take3 microvolume plate. Synergy H1.

Time-resolved fluorescence energy transfer Fluorescence polarization

cAMP assay
2500

2000
TR-FRET ratio

1500

1000

500

0
1.0E-11 1.0E-10 1.0E-09 1.0E-08 1.0E-07 1.0E11

[cAMP]
Time-resolved fluorescence energy transfer (TR-FRET) and homogeneous Fluorescence polarization is widely used in research labs to study mo-
time-resolved fluorescence (HTRF) are sensitive, robust methods. Synergy lecular binding or dissociation events, and in screening labs to screen
H1 and Gen6 provide excellent sensitivity for optimal Z-factors. for drug candidates.

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Bioluminescence resonance energy transfer Enzyme kinetics

Lineweaver-Burke plot
0.30
Protein X Protein Y 0.25

0.20

0.15

1/ V
NanoLuc HN HT 0.10

0.05
+ substrate
0.00
-5 -4 -2 0 2 4 5 6 10 12 14
Fluorescence 1/ [ S ]

Bioluminescence resonance energy transfer (BRET) proximity assays Enzyme reaction rates can easily be measured with Synergy H1. Gen6
enable detailed investigations of protein—protein interactions. BRET is data analysis software has built-in protocols for kinetic reactions.
easily detected with Synergy H1.

Protein aggregation Cell-based assays

Protein stress
pH, temperature, buffers, freeze/thaw, light, oxygen, mechanical

Folded protein Misfolded protein

Dye binds
to fibril and
fluoresceses

Add
Protein ProteoStat Protein
Dye
fibril aggregate

Synergy H1 has a robust shaking mechanism needed to quantify protein Cell-based assays assess critical characteristics such as viability, toxicity,
aggregation and amyloid formation via kinetic fluorescent measurements proliferation, and cell death.
of thioflavin T.

Metabolic activity Reactive Oxygen Species

Oxygen consumption
signal change H2DCFDA
(Non-fluorescent)

ROS
Time
DCF
(Fluorescent)

Use Agilent MitoXpress and pH-Xtra kits to measure real-time metabolic The formation of Reactive Oxygen Species (ROS) can be measured with
markers such as Oxygen Consumption Rates (OCR) and Extracellular the use of fluorescent probes in the Synergy H1.
Acidification Rates (ECAR).

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Peripherals

BioStack microplate stacker

The Agilent BioTek BioStack microplate stacker manages up to 50
microplates for automated imaging or multimode operations, including
de and relidding of microplates used with cell-based assays.

BenchCel microplate handler

The Agilent BenchCel microplate handler is a compact, automated system
that can be integrated with a variety of Agilent BioTek instrumentation.
For liquid handling, the Agilent BioTek MultiFlo FX multimode dispenser,
406 FX and EL406 washer dispensers, 405 TS and 405 LS washers, and
ELx405 Select deep well washers can be combined. Agilent BioTek detection
instruments, including the Cytation 5 cell imaging multimode reader, Synergy
Neo2 hybrid multimode reader, Synergy H1 multimode reader, and Epoch 2
microplate spectrophotometer can also be added. In addition, the BenchCel
is compatible with a wide range of microplates, including deep-well plates.
The combined automated workflows enable a wide variety of applications.

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 Peripherals

BioSpa 8 automated incubator

The Agilent BioTek BioSpa 8 environmental controls and labware handling
capabilities facilitate long-term live cell kinetic imaging processes for up to
eight microplates and other labware.

CO2/O2 controller
The compact gas controller maintains control of CO2 and O2 levels in the
Synergy H1 to support live cell assays.
Dual-reagent injector
The dual-reagent injector module enables fast inject/read processes. Angled
injector tips protect cell monolayers from shear stress during injection.

Take3 microvolume plate

Measure multiple 2 μL samples at a time with the Take3 microvolume plate,
used with Synergy H1. Microvolume nucleic acid and protein quantification
made fast and easy, for up to 16 or 48 samples at a time.

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 Agilent BioTek Synergy H1

Technical Details

General

Detection Modes UV-Vis absorbance

Fluorescence intensity
Luminescence
Fluorescence polarization
Time-resolved fluorescence

Wavelength Selection Monochromators for fluorescence intensity, UV-Vis absorbance, and luminescence
Filters for fluorescence intensity, time-resolved fluorescence, fluorescence polarization, and filtered luminescence

Monochromator Bandwidth Fixed: 16 nm

Variable: from 9 to 50 nm, in 1 nm increments ("M2" configurations)

Read Methods End point, kinetic, spectral scanning, well-area scanning

MicroplateTypes 6- to 384-well plates

Other Labware Supported Take3 microvolume plates

Environmental Controls 4-Zone Incubation to 70 °C ("M2" configurations) or 45 °C, with Condensation Control; CO2/O2 controller available

Shaking Linear, orbital, double orbital

Automation BioStack and third-party automation compatible

BioSpa 8 automated incubator compatible
BenchCel microplate handler compatible

Software Gen6 data analysis software

Gen5 Secure enables 21 CFR Part 11 compliance (option)

Modularity and Configurability Synergy H1 has many available configurations; detection modules and peripherals can be added as laboratory
needs change

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This information is subject to change without notice.

© Agilent Technologies, Inc. 2023

Published in the USA, September 15, 2023
5994-2417EN