CLINICAL PARASITOLOGY Jose M. Duallo Jr., RMT Mary Grace C. Tayong, RMT
Property of Misamis University - College of Medical Technology
LEARNING OUTCOME Differentiate parasitism from other symbiotic relationships and the different types of parasite and host.
Property of Misamis University - College of Medical Technology
SYMBIOSIS – 2 or more species live closely together Types: • Mutualism – both organism (of different species) benefit from each other • Commensalism – one specie benefits without harming the other • Parasitism – one specie benefits at the expense of the other specie Parasitology defined PARASITES: are organisms that live on and obtain nutrients from another organism (HOST) -Study of parasites which: • INFECT – invasion IN the human body (intestine, blood, tissue, liver, lung etc.) • INFEST – invasion ON the human body (lice, ticks, etc.) Cause disease (diarrhea, fever, anemia, blindness, elephantiasis, etc.) in the human body. Transport carriers known as VECTORS are always responsible for transmission to the host. Types of Parasites According to location • Endoparasite – established inside the host (eg. Ascaris lumbricoides, Plasmodium falciparum, Shistosoma japonicum) • Ectoparasite – established in or on the exterior surface of the host (eg. According to taxonomic groups • Protozoans – unicellular microorganism (eg. Amebae, Flagellates, Ciliates, Sporozoans) • Helminths – worms, multicellular organisms (eg. Nematodes, Cestodes, Trematodes) • Arthropods – Class Insecta Types of Parasites, cont. According to Degree of Association with Host • Obligatory – cannot survive outside the host • Facultative – capable of existing independently of a host • Permanent - remain in the host from early life to maturity • Temporary/ Intermittent – live on host for a short time • Accidental/ Incidental – enter host not usually theirs • Erratic/ Aberant – found in an organ not its usual habitat Types of Host • Definitive – host in which the adult sexual phase of parasite development occurs • Accidental/Incidental – host other than the normal one that is harbouring the parasite • Intermediate – host in which the larval asexual phase of parasite development occurs • Reservoir – host harbouring parasites that are parasitic for humans and from which humans may become infected • Paratenic – host harbouring the parasite in an arrested state of development, capable of continuing life cycle in a subsequent suitable host Types of Vectors 1. BIOLOGIC – in which parasites increase their numbers by multiplication or transformation inside the body of the carrier-organisms. (eg. Aedes Mosquito) 2. MECHANICAL/ PHORETIC – only transmits the parasite (eg. Flies, cockroaches) Parasitic Life Cycles 1. MODE OF TRANSMISSION 2. INFECTIVE STAGE – a morphologic form that invades humans 3. DIAGNOSTIC STAGE – 1 or more forms that can be detected in the lab Source of Infection • Contaminated Soil and Water • Food • Arthropods • Another person • Environment • Ownself Mode of Infection • Mouth • skin penetration • Congenital transmission • Inhalation • Sexual Intercourse Stages of Parasitism 1. Contact 2. Entry 3. Migration 4. Establichment 5. Development 6. Reproductive 7. Exit SPECIMEN COLLECTION AND PROCESSING Specimen Collection and Lab Diagnosis • STOOL • Cellophane Tape Prep • BLOOD • Permanent stains, Knott Technique, Buffy Coat Slides • TISSUE BIOPSIES • SPUTUM • URINE & GENITAL MATERIAL • COLON MATERIAL • CSF • EYE SPECIMEN • SKIN SNIPS Specimen Collection and Lab Diagnosis • STOOL – most commonly submitted - placed in a tight-fitting lid with an acceptable amount of stool ( 2-5 grams or about a walnut) - specimen container must be labeled : (a) Patient’s name and ID Number (b) Physician’s Name (c) Date and Time of sample collection Specimen Collection and Lab Diagnosis cont. FIXATIVES – substances that preserve the morphology of protozoa and prevent further development of certain helminth eggs and larvae. (eg. Formalin, Polyvinyl Alcohol, Sodium Acetate Formalin) 3 parts Fixative + 1 part Stool PROCESSING 1. MACROSCOPIC 2. MICROSCOPIC – ocular micrometer may be inserted in eyepiece • Direct Wet Preparation • Concentration Method ( eg. FEA Sedimentation, ZS Flotation) • Permanent Stains