1

Practical Subject

KARNATAK
KARNATAKUNIVERSITY, DHARWAD
UNIVERSITY, DHARWAD

04 - Year B.Sc. (Hons.) Program

***
B.Sc. Programme
SYLLABUS
***
Subject: Biotechnology
DRAFT SYLLABUS FOR
[Effective from 2021-22]
CHEMISTRY
DISCIPLINE
DISCIPLINE SPECIFIC
SPECIFIC COURSE
CORE COURSE (DSC)FOR
(DSCC) and SEM I & II,

DISCIPLINE
OPEN SPECIFIC
ELECTIVE ELECTIVE(DSE)
COURSE (OEC) FOR SEM I & II and
UNDER
SKILL ENHANCEMENT COURSE (SEC) FOR SEM I
NATIONAL EDUCATION POLICY(NEP)

*****

Effective from 2021-22

AS PER N E P - 2020

2
 Karnatak University, Dharwad
Four Years Under Graduate Program in Biotechnology for B.Sc. (Hons.)
Effective from 2021-22

Sem Type of Theory/ Instruction Total Duration Formative Summat Total Credits
Course Practical hour per hours of of Exam Assessme ive
Marks
Syllabus nt Marks
week Assess
/ Sem ment
Marks

I DSCC 1 Theory 04hrs 56 02 hrs 40 60 100 04

Practical 04 hrs 52 03 hrs 25 25 50 02
OEC-1 Theory 03 hrs 42 02 hrs 40 60 100 03

*SEC-1 Practical 03 hrs 30 02 hrs 25 25 50 02

II Theory 04 hrs 56 02 hrs 40 60 100 04
DSCC2
Practical 04 hrs 52 03 hrs 25 25 50 02
OEC-2 Theory 03 hrs 42 02 hrs 40 60 100 03

Details of the other Semesters will be given later

* Student can opt digital fluency as SEC or the SEC of his/ her any one DSCC selected

3
 Name of Course (Subject):B.Sc., (Basics/Hons) Biotechnology
Programme Specific Outcome (PSO):
On completion of the 03/ 04 years Degree in B.Sc., (Basics/Hons) students will be able to:

PSO 1 : Understanding concepts of Biotechnology and demonstrate interdisciplinary skills acquired

in cell biology, genetics, biochemistry, microbiology, and molecular biology

PSO 2 : Demonstrating the Laboratory skills in cell biology, basic and applied microbiology with an
emphasis on technological aspects

PSO 3 : Competent to apply the knowledge and skills gained in the fields of Plant biotechnology,
animal biotechnology and microbial technology in pharma, food, agriculture, beverages, herbal
and nutraceutical industries.

PSO 4 : Critically analyze the environmental issues and apply the biotechnology knowledge gained for
conserving the environment and resolving the problems.

PSO 5 : Demonstrate comprehensive innovations and skills in the fields of biomolecules, cell and
organelles, molecular biology, bioprocess engineering and genetic engineering of plants,
microbes, and animals with respect to applications for human welfare.

PSO 6 : Apply knowledge and skills of immunology, bioinformatics, computational modelling of

proteins, drug design and simulations to test the models and aid in drug discovery.;

PSO 7 : Critically analyze, interpret data, and apply tools of bioinformatics and multi omics in
various sectors of biotechnology including health and Food.

PSO 8 : Demonstrate communication skills, scientific writing, data collection and interpretation
abilities in all the fields of biotechnology. Demonstrate entrepreneurship abilities, innovative
thinking, planning, and setting up small-scale enterprises or CROs.

4
 B.Sc. Semester – I
Subject: Biotechnology
Discipline Specific Course (DSC)

The course B.Sc., (Basic/Hons) in I semester has two papers (Theory Paper –I for 04 credits & Practical Paper
-II for 2 credits) for 06 credits: Both the papers are compulsory. Details of the courses are as under.

Course No.-1 (Theory)

Course Type of Theory / Credits Instruction Total No. of Duration Formative Summative Total
No. Course Practical hour per Lectures/Hours of Exam Assessment
Assessmen Marks
week / Semester Marks
t Marks

Course-
01
DSCC Theory 04 04 56 hrs 2hrs 40 60 100

Course No.1 (Theory): Title of the Course (Theory): FUNDAMENTALS OF BIOTECHNOLOGY

Course Outcome (CO):

After completion of course (Theory), students will be able to:

CO 1 : Understanding concepts of Biotechnology and demonstrate interdisciplinary skills acquired in

cell biology, genetics, biochemistry, microbiology, and molecular biology

CO 2 : Understanding of basic structure of cell and its functions

CO 3 :Understanding of basic of genetic s and it application in basic biology

Syllabus- Course 1(Theory): Title- FUNDAMENTALS OF BIOTECHNOLOGY Total Hrs: 56

Unit-I 14 hrs
Chapter No. 1 Biotechnology: A Brief Introduction. Historical Advancement of 3
Biotechnology, branches of Biotechnology
Chapter No. 2 Introduction to the living world: Cell theory, Cell ultra-structure and 5
functions of cell, Different types of cells. Difference between prokaryotic and
eukaryotic cells; Difference between animal and plant cell. (plant and Animal).
Chapter No. 3 Ultra-structure of cell organelles Cell wall, Plasma membrane, 6
Mitochondria, Chloroplast, Ribosomes, Golgi complex. Endoplasmic reticulum.
Nucleus, Lysosomes. Peroxisomes, Vacuole. Cytosol and Cytoskeletal structures
Unit-II 14 hrs
Chapter No. 4. Concept of genome and Chromosomes Discovery Morphology and 6
structural organization: number, size and, fine structure, heterochromatin and
euchromatin. Special types: giant chromosomes (Salivary gland and Lampbrush).

Chapter No. 5. Cell study techniques. Fixatives, Principles of staining, Types of 4

stains, Auxochromes.

Chapter No. 6. Cell cultivation methods - Nutritional requirements (Bacteria and 4

plant), laboratory facilities, growth curve, Single cell suspension cultures and their
applications.

5
Unit-III 14 hrs
Chapter No. 7 Science of Genetics-Mendel's Laws of heredity, Mono and di-hybrid 11
crosses, Test-cross, Incomplete dominance and multiple allelism. Cell cycle, mitosis and
meiosis, mitotic apparatus, centrioles, spindles, cell plate formation, cell synchrony and
significance. Amoeboid, flagella and ciliary motility, Spermatogenesis and Oogenesis.
Chapter No. 8. Cells for cloning: E.coli, Bacillus subtilis, Saccharomyces cerevisiae. 3
Unit-IV 14 hrs
Chapter No. 9. Concept of Genetic Engineering: Definition; Tools used in 9
recombinant DNA Technology: Plasmids, Transgenesis: Production and significance of
transgenic plants (Golden rice) and animals (sheep); the Basic concept of genetically
modified organisms.
Chapter No. 10. Role of biology in allied fields: Role of biology in Information 5
Technology (Bioinformatics), Nanotechnology (Nanobiotechnology), Micro-
electromechanical systems (Bio-MEMS), and Sensors (Biosensors)

Books recommended.

1. Sudberry P. 2002, Human Molecular cytogenetics. Prentice hall publication

2. Knudson A.G. 1998, Anti – Oncogenes and Human cancer. Proceedings of the National academy of
sciences USA 90: 10, 0114 – 10921
3. Lodish, H., Ber, A., Zipursky, L.S., Matsudaira, P., Bahimore, D and Darnell J. 2001,
Molecular Biology W. H. Freeman G Co 47
4. Preeti G. 2011, Fundamentals of Biotechnology. Galgotia Publications.
5. Sabiha Khan. 2020, Fundamentals of Biotechnology.Lenin Media Pvt. Ltd.
6. Firdos A. K. 2020,Biotechnology Fundamentals. Third Edition CRC Press.
7. Bazlur Rashid. M. 2016, Methods in Biotechnology1st edition Wiley-Blackwell.
8. B. Alberts. D. Bray, J. Lewis, M. raff K, Roberts and J. D. Watson 2008 Molecular
9. Biology of the Gene. 2012, Garland Publisher Inc., New York
10. Gerald Karp 2004, Cell and Molecular Biology. John Wiley and Sons. Inc
11. Elliot and Elliot, 2001 Biochemistry and Molecular Biology. Oxford University Press.
12. Celis J E (Eds): 2008 Cell Biology: A Laboratory Hand Book. Vol I & II Academic Press.
13. Pollard J.P. and W.C. Earnshow 2002. Cell Biology, Sunders
14. Tamarin, R.H, (2000): Principles of genetics, 6th Edn. WMC Brown Publication.
London.
15. Snustad, P.D, Simmons, M.J 2019: Principles of genetics 2nd Edn. John Wiley and sons,Inc. New
York.
16. Fairbanks, D.J and Anderson, W.R 1999: Genetics – continuity of life. Brooks and Cole
Publication Company. New York.
17. Lewin, B (2020): GENES VII. Oxford University Press, New York.
18. Strickberger, M.W (2000): Genetics Prentice - Hall of India private limited, New Delhi.
19. Miglani G.S 2000 Basic Genetics Narosa publishing New Delhi.

6
 B.Sc. Semester – I
Subject: Biotechnology
Discipline Specific Course (DSC)

Course No.-1 (Practical)

Course Type of Theory / Credits Instruction Total No. of Duration Formative Summative Total
No. Course Practical hour per Lectures/Hours of Exam Assessment
Assessmen Marks
week / Semester Marks
t Marks

Course- Practic
01 DSCC 02 04 52 hrs 3hrs 25 25 50
al

Course No.1 (Practical): Title of the Course (Practical): FUNDAMENTALS OF BIOTECHNOLOGY

Course Outcome (CO):

After completion of course (Practical), students will be able to:

CO 1 : Learning and practicing the Laboratory skills in cell biology, basic and applied microbiology
with an emphasis on technological aspects

CO 2 : Learning and practicing the concepts of Biotechnology and practical skills in interdisciplinary
skills acquired in cell biology, genetics, biochemistry, microbiology, and molecular biology

List of the Experiments for 52 hrs / Semesters

1. Study of fixatives and stains: Preparation of formaldehyde (4-10%), Alcohol (70%),

Bouin’s fixatives, Carnoy’s solution, Borox carmine (alcoholic), Eosin (alcoholic), Iron
(Haematoxylin). Leishman’s Stain, Ringer’s Solution, Acetocarmine, Acetoorcein,Schiff’s reagent
(Feulgen), Giemsa’s stain.
2. Mitochondrial staining
3. Micrometry
4. Cell counting methods: Heamocytometer and other aids.
5. Squash preparation of onion root tips to study stages of mitosis.
6. Smear preparation of grasshopper testes and flower buds to study stages of meiosis.
7. Smear preparation to study salivary gland chromosomes
8. Buccal epithelial smear and Barr body observation.
9. Extraction of cellular materials in saline buffers, solvents and precipitation.
10. Demonstration of laws of inheritance by using colour beads
a. Laws of segregation
b. Laws of independent assortment.
c. Solve genetic problems
11. Study of different types cells using permanent slides
12. Spotters

7
General instructions:

Scheme of Practical Examination (distribution of marks): 25 marks for Semester end examination

1. Major Question ---------------------- 10 Marks

2. Minor Question ---------------------- 06 Marks

3. Identify and comment --------------- 3X1 = 03 Marks

4. Viva-------------------03Marks

5. Journal--------------- 03Marks

Total 25 marks

Note: Same Scheme may be used for IA( Formative Assessment) examination

Books recommended.
1. Preeti G. 2011, Fundamentals of Biotechnology. Galgotia Publications.
2. Sabiha Khan. 2020, Fundamentals of Biotechnology.Lenin Media Pvt. Ltd.
3. Firdos A. K. 2020,Biotechnology Fundamentals. Third Edition CRC Press.
4. Bazlur Rashid. M. 2016, Methods in Biotechnology1st edition Wiley-Blackwell.

8
 B.Sc. Semester – I
Subject: Biotechnology
Open Elective Course (OEC-1)
(OEC for other students)

Course Type of Theory / Credits Instruction Total No. of Duration Formative Summative Total
No. Course Practical hour per Lectures/Hours of Exam Assessment
Assessmen Marks
week / Semester Marks
t Marks

OEC-1
OEC Theory 03 03 42 hrs 2hrs 40 60 100

OEC-1: Title of the Course: Biotechnology for human welfare

Course Outcome (CO):

After completion of course, students will be able to:

CO 1 :Understanding importance of Genetic engineering in Industry and bio-therapeutic proteins

CO 2 :Applications of molecular Genetics in Forensic science to solve criminal cases by DNA

analysis

Syllabus- OEC: Title- Biotechnology for human welfare Total Hrs: 42

Unit-I 14 hrs
Industry: Protein engineering; enzyme and polysaccharide synthesis, organic
compounds, alcohol and antibiotic synthesis
Environment: Application of biotechnology in environmental aspects. Bioremediation,
solid and liquid waste management.
Unit-II 14 hrs
Forensic science: DNA finger printing, Solving violent crimes such as murder and
rape; solving claims of paternity and theft etc. using various methods.
Unit-III 14 hrs
Health: Biopharmaceuticals, development of non-toxic therapeutic agents, recombinant
vaccines, insulin, gene therapy, molecular diagnostics using ELISA, PCR, monoclonal
antibodies and their use in diagnostics and therapy, human genome project
Reproductive Biotechnology: In vitro fertilization, IUI, nuclear transfer, embryo
Transfer
Books recommended.
1. H.K.Dass (2018)Text book of Biotechnology., Wiley India publication
2. B.D.Singh (2017)Biotechnology new horizon., Kalyani Publishers
3. R.C.Dubey (2015)Text book of Biotechnology, S.Chand and company
4. U. Satyanarayan (2005) Biotechnology. BOOKS & ALLIED (P) LTD.-KOLKATA.
5. W.T. Godbey(2014)An Introduction to Biotechnology, 1st Edition Academic Press.
6. K. Pranav, V. Praveen, M. Usha (2017). Biotechnology A Problem Approach, Fifth edition
Pathfinder Publications.
7. Rup Lal (2020). An Introduction to Biotechnology - A Genetic Manipulation Perspective, First
Edition Dreamtech Press

9
 B.Sc. Semester - I
Subject: Biotechnology
SKILL ENHANCEMENT COURSE (SEC)-I

Title of Paper: BIOTECHNOLOGICAL SKILLS

Theory / Credits Instruction Total No. of Mode of Duration of Formative Summative Total
Type of
Course Practical hour per Lectures/Hours Examina Exam Assessment
Assessmen Marks
week / Semester tion Marks
t Marks

Theory + 2hr
SEC-I 02 03hrs 30 Practical 25 25 50
Practical

Content of Course 1 Total 30

(one hour theory followed by 2 hours practicals) Hrs

Unit –1 7
Rules and regulation, Safety measures in Biotechnology laboratory.
Titrimetric Analysis, Concept of Molarity, Normality, Preparation of solutions
of different Molarity/Normality of titrants, Preparation of reagents.

Unit –2 7
Concept of Buffer and pH, its importance in Biological experiments,
Preparation of buffer solutions of different pH. Sodium acetate-acetic acid.
Phosphate buffer etc Preparation and Sterilization of Glassware and media
1. Calculation of Molarity, Normality and Molality
2. Calibration of pH meter using standard buffers
3. Preparation of Buffers- Phosphate Buffer, Acetate Buffer and TRIS
Buffer.
Unit- 3 7
Study of Biotechnology related laboratory instruments. Understanding principles
of general lab instruments: Autoclave, hot air oven, incubator, Laminar Air Flow,
Centrifuge, Colorimeter and Spectrophotometer. Colorimetric estimation of protein
by Biuret method/ Barford’s method.
Unit –4 9
Principles of chromatography. Types of chromatography, Paper
chromatography and applications.
Demonstration of Chromatographic techniques (Paper Chromatography of amino
acids)
Extraction of protein from plant and animal source.
Salt precipitation and organic solvent methods.
Protein estimation.

10
Books Recommended:

1. H.K.Dass (2018)Text book of Biotechnology., Wiley India publication

2. B.D.Singh(2017)Biotechnology new horizon., Kalyani Publishers
3. R.C.Dubey (2015)Text book of Biotechnology., S.Chand and company
4. William J. Thieman, Michael A. Palladino (2008), Introduction to Biotechnology Publisher:
Benjamin Cummings
5. Colin Ratledge(2012),Basic Biotechnology: Cambridge University Press
6. Boyer, R (2017) Concepts in Biochemistry. 5thint. edn – Brooks / Cole, Australia.
7. David Leader RLP Adams, John Knowler (2020) The Biochemistry of the nucleic acids. 11thedn
Chapman and Hall.
8. W.T. Godbey (2014)An Introduction to Biotechnology, 1st Edition Academic Press.
9. K. Pranav, V. Praveen, M. Usha (2017). Biotechnology A Problem Approach, Fifth edition
Pathfinder Publications.
10. M. Bazlur Rashid (2016). Methods in Biotechnology1st edition Wiley-Blackwell.

Course Outcome (CO):

After completion of Skill Enhancement course, students will be able to:

CO 1: Basic principles of reagents preparation and physic chemical nature and its application in
Human life

CO 2 : Understanding basic of chromatography and its role in Pharma Industries

Scheme of Practical Examination (distribution of marks): 25 marks for Semester end examination

1. Major Question ---------------------- 10 Marks

2. Minor Question ---------------------- 06 Marks

3. Identify and comment --------------- 3X1 = 03 Marks

4. Viva-------------------03Marks

5. Journal--------------- 03Marks

Total 25 marks

11
 Details of Formative assessment (IA) for DSCC theory/OEC: 40% weight age for total marks

Type of Assessment Weight age Duration Commencement

Written test-1 10% 1 hr 8th Week
Written test-2 10% 1 hr 12th Week
Seminar 10% 10 minutes --
Case study / Assignment / 10% ------ --
Field work / Project work/
Activity
Total 40% of the maximum
marks allotted for the
paper

Faculty of Science
04 - Year UG Honors programme:2021-22

GENERAL PATTERN OF THEORY QUESTION PAPER FOR DSCC/ OEC

(60 marks for semester end Examination with 2 hrs duration)

Part-A
1. Question number 1-06 carries 2 marks each. Answer any 05 questions : 10marks

Part-B

2. Question number 07- 11 carries 05Marks each. Answer any 04 questions : 20 marks

Part-C

3. Question number 12-15 carries 10 Marks each. Answer any 03 questions : 30 marks

(Minimum 1 question from each unit and 10 marks question may have sub
questions for 7+3 or 6+4 or 5+5 if necessary)

Total: 60 Marks

Note: Proportionate weight age shall be given to each unit based on number of hours prescribed.

12
 B.Sc. Semester – II
Subject: Biotechnology
Discipline Specific Course (DSC)

The course B.Sc., (Basic/Hons) in I semester has two papers (Theory Paper –I for 04 credits & Practical paper-
II for 2 credits) for 06 credits: Both the papers are compulsory. Details of the courses are as under.

Course No.-2 (Theory)

Course Type of Theory / Credits Instruction Total No. of Duration Formative Summative Total
No. Course Practical hour per Lectures/Hours of Exam Assessment
Assessmen Marks
week / Semester Marks
t Marks

Course-
02
DSCC Theory 04 04 56 hrs 2hrs 40 60 100

Course No.2 (Theory): Title of the Course (Theory): Microbiological methods

Course Outcome (CO):

After completion of course (Theory), students will be able to:

CO 1 : Understanding of Microbes and their handling , culture methods, etc.,

CO 2 : Learning of various methods for identification of microbes.

CO 3 : Microbiological diseases from bacteria, fungi and virus, its pathological significance

Syllabus- Course 2(Theory): Title- Microbiological methods Total Hrs: 56

Unit-I 14 hrs
Chapter No. 1 Introduction and Scope of Microbiology: Historical perspectives. 3
Importance and scope of microbiology as a modern science. Branches of microbiology
and allied sciences.

Chapter No. 2 Sterilization techniques: -Physical, dry heat, moist heat, chemical and 4
radiation methods of sterilization. pasteurization, Tyndalization, Filtration, HEPA,
Principles and Applications. disinfection sanitization, antisepsis and fumigation

Chapter No. 3 Stains and staining techniques. Bacterial and fungal staining - Simple, 3
Differential and Structural staining. Capsule, cell wall, flagella & endospore staining.

Chapter No. 4 Microbial taxonomy: Concept of microbial species and strains, 4

Taxonomical characteristics of microorganisms, Classification of bacteria based on –
morphology, Molecular identification of microbe, numerical taxonomy, Bergey’s
manual
Unit-II 14 hrs
Chapter No. 5. Ultra-structure of virus, bacteria and yeast: Typical bacteria, cell 3
wall structure, flagella structure, plasmid, tobacco mosaic virus (TMV), Bacteriophage-
λ phage, Saccharomyces cerevisiae.

13
Chapter No. 6. Culture of microorganisms: Culture media, Types of culture media, 4
Preservation and sub culture of media. Lyophilization and long term storage media

Chapter No. 7. Microbial growth: Nutritional requirements of microorganisms. 3

Bacterial growth curve. Factors influencing growth, Counting of Bacteria.

Chapter No. 8. Pathogenic microorganisms: Bacterial diseases of humans - Tetanus, 4

Tuberculosis and Cholera, Viral diseases –HIV (AIDS), Hepatitis, Ebola & Corona
virus.
Unit-III 14 hrs
Chapter No. 9. Applied Microbial methods: 4
Environmental Microbial methods- Quality of air, water and soil sampling methods
Food and dairy Microbial Methods- Food quality, health and hygiene of food
Industrial Microbial methods- Microbial measurements and limits in industrial
assessments.
Chapter No. 10. Introduction to Immunology: History and Scope of Immunology, 3
Types of Immunity- Innate, active, passive and acquired immunity. Humoral and cell -
mediated immunity Organs of the Immune system: Bone marrow. Thymus, lymph
node and spleen.
Chapter No. 11. Antigens and Antibodies: Types of antigens, Super antigens, haptens, 7
epitopes, paratope, Role of Adjuvant in immunogenicity (Fraud’s Complete Adjuvant
and Fraud’s Incomplete Adjuvant) Blood group antigens; Antibody Structures, types,
properties and functions of immunoglobulins
Unit-IV 14 hrs
Chapter No. 12. Advanced Microbial Methods: Identification and sequencing of 7
unknown microorganisms (Culturable and non-culturable). High throughput sequencing.
Mechanism of immune-precipitation, Agglutination, Complement fixation, Mechanism
of immuno-toxin reaction, Immuno-blotting, Immuno-Fluorescence
Chapter No. 13. Hypersensitivity: Types of hypersensitivity - IgE mediated (type - I). 7
Antibody mediated cyto-toxic (type-II), Immuno complex mediated (type-III) and T-
mediated (type-IV) hypersensitivity reactions.

Books recommended.
1. Ananthanarayan R. JayarmanPaniker 2005: Textbook of Microbiology. 7th Edition, Orient
Longman publication
2. Aneja K R 2017: Experimental Microbiology Plant Pathology and Biotechnology. 5th Edition,
New age International. New Delhi
3. Ronald M Atlas, Richard Bartha, and David Atlas 1998: Microbial Ecology: Fundamentals and
application 2nd Edition McMillan publishing Co. New York
4. Joanne Willey and Kathleen Sandman and Dorothy Wood Eleventh edition.(2020). “Prescott's
Microbiology”. New York, NY : McGraw-Hill Education.
5. Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl and
Thomas Brock. (2021). “Brock Biology of Microorganisms”, 14th Edition. Pearson.
6. Peleczar, M.J.. Chan. E.C.S and Krieg, N.R. (2020)"Microbiology"7th Edition. Tata MaGraw Hill
Book.
7. Gerard J. Tortora, Berdell R. Funke and Christine L. Case (2021). Microbiology: An Introduction,
13th Edition. Pearson Education.
8. Ronald M Atlas, 1988: Microbiology: Fundamentals and application 2nd Edition
McMillan publishing Co. New York
9. Ronald M Atlas 2005 Handbook of Media for Environmental Microbiology 2nd
Edition, Taylor & Francis
14
10. Auro, P.T Kapoor, K.K. Yadav, K.S 2019: An introduction to Microbiology, 3rd
Edition, New Age International Pvt. Ltd.
11. Gerhardt, R.G.E. Murray, W.A. Wood and N.R. Krieg 1996: Methods for general and
12. molecular bacteriology. American Society for Microbiology. Washington DC.
13. Bergey, D. H., Boone, D. R., Staley, J. T., Garrity, G. M., Krieg, N. R., De
14. Vos, P., Goodfellow, M., Brenner, D. J., Castenholz, R. W. (2001). Bergey's Manual® of Systematic
Bacteriology: Volume Two: The Proteobacteria (PartC). Germany: Springer.
15. Kumar, H. D and Swati Kumar 2004: Modern concepts of Microbiology. 2nd Edition,
Vikas Publishing House Pvt. Ltd. New Delhi
16. Michael J Pelczar, JR, E.C.S Chan, Noel R Krieg 2015: Microbiology – Tata McGraw
Hill Publication

15
 B.Sc. Semester – II
Subject: Biotechnology
Discipline Specific Course (DSC)

Course No.-2 (Practical)

Course Type of Theory / Credits Instruction Total No. of Duration Formative Summative Total
No. Course Practical hour per Lectures/Hours of Exam Assessment
Assessmen Marks
week / Semester Marks
t Marks

Course- Practic
02 DSCC 02 04 52 hrs 3hrs 25 25 50
al

Course No.2 (Practical): Title of the Course (Practical): Microbiological methods

Course Outcome (CO):

After completion of course (Practical), students will be able to:

CO 1 : Understanding of Microbes and their handling , culture methods, etc.,

CO 2 : Understand and identification of methods for microbes.

CO 3 : Analyze microbial diseases from bacteria, fungi and virus, its pathological significance

List of the Experiments for 52 hrs / Semesters

1. Safety measures in microbiology laboratory

2. Cleaning and sterilization of glass wares
3. Study of instruments: Compound microscope, Autoclave, Hot air oven, pH meter, Laminar airflow,
centrifuge, colony counter etc
4. Media preparation : Nutrients agar, Nutrient broth and potato dextrose agar
5. Inoculation technique: Stab, point, Streak, pour plate and spread plate
6. Isolation of bacteria and fungi from soil, air, and water – dilution and pour plate
methods and study of colony characters
7. Bacterial staining techniques -simple and differential (Gram’s)
8. Counting of micro-organisms – Total count (Haemocytometer)
9. Biochemical tests – Starch hydrolysis, catalase, Gelatin liquification
10. Preparation of bacterial and fungal antigens
11. Total RBC and WBC count
12. Estimation of haemoglobin in blood
13. Demonstration of ELISA and RIA
14. Study of Rocket electrophoresis

16
General instructions:

Scheme of Practical Examination (distribution of marks): 25 marks for Semester end examination

1. Major Question ---------------------- 10 Marks

2. Minor Question ---------------------- 06 Marks

3. Identify and comment --------------- 3X1 = 03 Marks

4. Viva-------------------03Marks

5. Journal--------------- 03Marks

Total 25 marks

Note: Same Scheme may be used for IA( Formative Assessment) examination

Books recommended.
1. Aneja K R 2017: Experimental Microbiology Plant Pathology and Biotechnology. 5th Edition,
New age International. New Delhi
2. Ronald M Atlas, Richard Bartha, and David Atlas 1998: Microbial Ecology: Fundamentals and
application 2nd Edition McMillan publishing Co. New York
3. Joanne Willey and Kathleen Sandman and Dorothy Wood Eleventh edition.(2020). “Prescott's
Microbiology”. New York, NY : McGraw-Hill Education.
4. Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl and
Thomas Brock. (2021). “Brock Biology of Microorganisms”, 14th Edition.Pearson.
5. Peleczar, M.J.. Chan. E.C.S and Krieg, N.R. (2020)"Microbiology"7th Edition. Tata MaGraw
HillBook.

17
 B.Sc. Semester – II
Subject: Biotechnology
Open Elective Course (OEC-2)
(OEC for other students)

Course Type of Theory / Credits Instruction Total No. of Duration Formative Summative Total
No. Course Practical hour per Lectures/Hours of Exam Assessment
Assessmen Marks
week / Semester Marks
t Marks

OEC-2
OEC Theory 03 03 42 hrs 2hrs 40 60 100

OEC-2: Title of the Course: Applications of Biotechnology in Agriculture

Course Outcome (CO):

After completion of course, students will be able to:

CO 1 : Competent to apply the knowledge and skills gained in the fields of Plant biotechnology, in
pharma, agriculture, herbal and nutraceutical industries.

CO 2 : Application of Bio based nutrient and growth promoters for agriculture yields.

Syllabus- OEC: Title- Applications of Biotechnology in Agriculture Total Hrs: 42

Unit-I 14 hrs
Agricultural Biotechnology: Concepts and scope of biotechnology in
Agriculture. Plant tissue culture, micro propagation, entrepreneurship in commercial
plant tissue culture. Banana tissue culture - primary and secondary commercial setups
,Small scale bioenterprises: Mushroom cultivation
Unit-II 14 hrs
Transgenic plants: The GM crop debate – safety, ethics, perception and acceptance of
GM crops GM crops case study :Bt cotton, Bt brinjal Plants as bio-factories for
molecular pharming : edible vaccines, nutraceuticals
Unit-III 14 hrs
Bio-pesticides: Baculovirus pesticides, Mycopesticides, Post-harvest Protection :
Antisense RNA technology for extending shelf life of fruits and shelf life of flowers.
Genetic Engineering for quality improvement: Seed storage proteins, Flavours–
capsaicin, vanillin.

18
Books recommended.
1. Primrose. S.B. and Twyman R.M. Principles of gene manipulation and genomics. Seventh Edition. 2006.
Blackwell Publishing, Australia.
2. Sandhya Mitra. (1996). Genetic Engineering. Principles and Practice. Macmillan India Ltd, New Delhi.
3. Das. H.K. (2007). Textbook of Biotechnology. Third Edition. Wiley India Pvt Ltd, New Delhi.
4. David A Micklos and Greg A Freyer. (2005). DNA Science, a first course. Second Edition. I.K. International Pvt
Ltd, New Delhi.
5. Gupta. P.K. Biotechnology and Genomics. 2008. Rastogi Publications, New Delhi.
6. Winnacker E.L. (1987) From Genes to clones, Introduction to gene technology. VCH, Verlagsgesellschaftmbh,
Whinheim, Germany.
7. Channarayappa. (2006) Molecular Biotechnology, Principles and Practices. University press (India) Pvt. Ltd,
Hyderabad, India.
8. Becker. J.M, Caldwell.GA, Zacgho. E.A. (1996) Biotechnology, A laboratory Course. Second Edition..
Academic Press. INC, California.
9. Principles of Gene Manipulations (1994) by Old and Primrose Blackwell Scientific Publications.
10. DNA Cloning: A Practical Approach by D.M. Glower and B.D. Hames, IRL Press, Oxford. (1995).
11. Molecular Biotechnology 2ndEdition by S.B. Primrose. Blackwell Scientific Publishers, Oxford. (1994).
12. S.M. Kingsman and A.J. Kingsman (1998) Genetic Engineering and Introduction to Gene Analysis and
Exploitation in Eukaryotes by, Blackwell Scientific Publications, Oxford
13. PCR Technology - Principles and Applications for DNA Amplification by Henry A. Erlich (Ed.) Stockton Press.
(1989).
14. Biotechnology: A Guide to Genetic Engineering by Peters.
15. Genetic Engineering – (2000) by Nicholl.
16. Recombinant DNA and Biotechnology: Guide for Teachers. 2nd Edition by Helen Kreuz. (2001). ASM
Publications
17. Molecular Biotechnology: Principles and Applications of Recombinant DNA. 2 nd Edition. (1998) by Bernard R.
Glick and Jack J. Pastemak, ASM Publications.
18. From genes to clones by Winnaker.
19. Manipulations and expression of recombinant DNA by Robertson.
20. Gene targeting – A practical approach by Joyner.
Details of Formative assessment (IA) for DSCC theory/OEC: 40% weight age for total marks

Type of Assessment Weight age Duration Commencement

Written test-1 10% 1 hr 8th Week
Written test-2 10% 1 hr 12th Week
Seminar 10% 10 minutes --
Case study / Assignment / Field 10% ------ --
work / Project work/ Activity
Total 40% of the maximum marks
allotted for the paper

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 Faculty of Science
04 - Year UG Honors programme:2021-22

GENERAL PATTERN OF THEORY QUESTION PAPER FOR DSCC/ OEC

(60 marks for semester end Examination with 2 hrs duration)

Part-A
1. Question number 1-06 carries 2 marks each. Answer any 05 questions : 10marks

Part-B

2. Question number 07- 11 carries 05Marks each. Answer any 04 questions : 20 marks

Part-C

3. Question number 12-15 carries 10 Marks each. Answer any 03 questions : 30 marks

(Minimum 1 question from each unit and 10 marks question may have sub
questions for 7+3 or 6+4 or 5+5 if necessary)

Total: 60 Marks

Note: Proportionate weight age shall be given to each unit based on number of hours prescribed.

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