NAME RIESHI KEISHI A/L SELVADURAI

MATRIC NUMBER MS2316115458

PRACTICUM H16
LECTURER’S NAME AFIQAH BINTI MOHAMED
DATE OF 20/2/2023
EXPERIMENT

TITLE CELLULAR RESPIRATION 1 mark

OBJECTIVE 1. To organize the experiment setting for redox reaction procedures 3 marks
2. To conduct an experiment on redox reaction in cellular respiration
3. To explain the biochemical processes in yeast suspension

INTRODUCTION 1. Explain the redox reaction.

A chemical reaction involving both reduction and 1 mark

oxidation, (which results in changes in oxidation numbers

of atoms included in the reaction)

1 mark

2. What is the substance in a living cell that has the same function as
methylene blue?
Hydrogen 1 mark

3. Name the important process which involves substance in question 2

above.
Oxidative phosphorylation

PROCEDURE 3 marks
1.3 boiling tubes was labelled as A,B and C.

2.10 ml of yeast suspension was filled tubes

3.Tube C was heated in boiling water for 5

minutes

4.5 drops of methylene blue was added into each of the boiling tubes
using Pasteur pipette and was gently shaked to ensure the colour is
evenly distributed

5. The colour of the yeast suspension was observed in all boiling

tubes and was recorded in Table 10.1
 6. All boiling tubes was incubated in the water bath at 40°C for 15
minutes

7. The colour changes of the yeast suspension was observed in all

boiling tubes and was recorded in Table 10.1

8.Tube B was placed in boiling water for 5 minutes

9.Boiling tube A,B and C was plugged with cork or rubber stopper.It was
pressed with thumb and was shaked vigorously for 30 seconds.The colour
changes of yeast suspension was observed and was recorded in Table 10.1
10.The stopper was removed and all boiling tubes was incubated in water
bath at 40°C for 15 minutes
11.The colour changes of the yeast suspension in each boiling tube was
observed and was recorded in Table 10.1.

RESULTS

Treatments Colour
Tube A Tube B Tube C
Before Blue Blue Blue 1 mark
incubation (400C)
After 1 st Light blue Light blue Blue 1 mark
0
incubation (40 C)
After vigorous Blue Blue Blue
shaking 1 mark
After 2 nd
Light blue Blue Blue 1 mark
incubation (400C)

DISCUSSION
Boiling Explanation
tube
A After 15 minutes/1 incubation
st

● Yeast used all dissolved oxygen // conduct aerobic

respiration
● When dissolved oxygen is used up, solution colour 3 marks
becomes lighter blue.
● Because methylene blue is reduced to form
decolourised methylene.
Remarks
**But remain blue at air – water interface because
dissolved O at the upper part causes methylene
2

blue to oxidize

After shaking 3 marks

● Shaking causes oxygen from the air to dissolve
into the solution.
● Methylene blue is oxidised.
● Changes the solution to blue colour.
 After 10 – 15 minutes/2 incubation
nd

Remarks
* Same explanation as in after 15 minutes

B After 15 minutes/1 incubation

st

Remarks
* Same explanation as in Tube A
● Yeast used all dissolved oxygen // conduct aerobic
respiration
● When dissolved oxygen is used up, solution colour
becomes lighter blue.
● Because methylene blue is reduced to form
decolourised methylene.
3 marks
After shaking
Remarks
* Same explanation as in Tube A
● Shaking causes oxygen from the air to dissolve
into the solution.
● Methylene blue is oxidised. 2 marks
● Changes the solution to blue colour.

After 10 – 15 minutes/2 incubation

nd

● Boiling causes the enzymes (in yeast) to

denatured// yeast cells dies
● Dissolved oxygen is not used.
● Methylene blue remains in oxidised form / cannot
be reduced
● The colour of solution unchanged / remain blue.

C ● No changes occur throughout the process.

● Because boiling causes the enzymes in yeast to
denatured
● Dissolved O is not used, methylene blue is in
2

oxidise form / remain blue

CONCLUSION 1. Methylene blue act as artificial electron acceptor

showing redox reaction has taken place due to the 1 mark
color changes

2. Methylene blue is reduced to form decolourised methylene 1 mark

3. Methylene blue is oxidised to change the solution to blue

colour. 1 mark

Reference Malaysia National Library,Biologi Laboratory Manual,semester I & II,SB015 & 1 mark
SB025 Thirteenth Edition
Submission date 1 mark
(not late)
Total /30