MAh

KOLEJ MATRIKULASI JOHOR

MINISTRY OF EDUCATION 84900, MARKS: Bioto)y
CREA
KMMJ TANGKAK JOHOR

NAME
RIESHI K6ISHI A, CELVADVI
MATRICNUMBER
Ms 23)6 i|5458
PRACTICUM
HI6
LECTURER'S NAME

DATE OF
ENPERIMENT
2477/n023

TITLE Imark
Basic TechniqueS o Microscopy
OBJECTIVE i) obtain accurute imises. 2 marks

) determung the dip depth o! ield

) deternine the Geld of view
v) Calculate the actual muqni fication
aeply
the use of ol immersion ith high eAghiicaton
(oil imenersjon lens
INTRODUCTIO What is a light compound microscope? 3 marks
Bn iostrumeat thet an be use to tocus and magnity small
Specimen
Give the formula to calculate the actual magnification
Maqni fi catin powrer y Maqgifcaton power Hctul
of obiective \ens of cular lens trighitcetion
What is the field of view?
The observable risible circular ccope when loolcing through
he microScope
3 marks
PROCEDURE Tmages under the microscope
The e' preecred slide ws obsered by using the hx oojecivelens.
9, wht was obsetved by using the Ax obicctive ers is drawn.
3. The position o e' was detemied.
The degth of Aed
. The position of the threud onhe clide s obseeved with naked eues
Th coour of thread ts was ideri ed a the toe,in tbL middle
and at botto
The Cmss threads as observeo under the microscope using 4x and
10% objecie lens.
as<d
3. The depth of Seld uhen the per of oblecthve lens incre
wes obcerved.
The Aeld ot iew
1:9 transpatent aler ves placed on the stoqe. he rulcr scale uas
placed ct the certte of the Rerd ot wiew
2. The trnsrareot ulee wos observed usir he A<,iox and
kox objectve lenses.
determined at h ox
3. The drameter of fed of view bos
and ox obecive ens
 oilimmersion obectte \ens
| The prepatcd clide under the icroscope using i004 objeeAivc
Tens s objerved.

2. The obsevction wus drawn

RESULTS Drawing of 'e' prepared slide 5marks

(DRAWINGS) Drawing of transparent ruler prepared slide
Drawing of prepared slide (bacterialplant tissue)

TAlei 'e' prepared slide Titlei Transparent ruler

Prcpared slide
Pcal meqnitetion* h0

o00
D

THle: Es chericha oli prepared slide

Actual magnification : l000 x
 RESULTS Position of cross thread: 5 murks
(TABLES) Location Naked eye
At the top reen
In the middle
Atthe bottom Black

Table I.1 Diameter field of view

Objective lens Diameter in mm Diameter in Om

4X 3-5 3500
10 X 1500
40 X 0-375 395

3 Table I.2 Actual magnification of aspecimen

Magnification power Magnification power of objective lens
of ocular lens 4x 10x 40x J00x
10x l00x 400 |000

DISCUSSION 1. Determine the position of 'e'. Give 2 reasons. 8marks

Inyerted because the curvature of the frst lens whch

inverts Ahe image and the Stcond kns has the image paus
througn it
2 Determine what happens to the depth of field when the power of
an objective lens increases. Explain why in terms of focal length.
Decreases, becuuse if subject disfance stays the
chort
Same lens qive less depth than a
a long
3 Compare location of threads observed under naked eye and
microscope
Ne observe 3 overlappirg dhreads of ditferent Colour but
th rough light microscope, theTocation of Cross hreuds is the sarme:
4 State what happens to image size when observed under
microscope.
The imaqe si2e will be magnfied
uneer a microsp
State the significance why some cells need to be stained
o enhunce neualilaton of the cel| or certuin celluar components
CONCLUSION -The micro scope uas handied properly 3 marks

la. Tmaqes seen under a microso pe are invertedpower and laaer

maqnitication is 4he maanifcetion of an
a. The actal f an 0culur leng
bjectire jens x the magnitcaofens p the depth of field inras
A Ms te pouer of an objectre
5- s the power of an objectve lers ncreascs, the fel8 o riew deereuses
QUESTIONS Part A 15 marks
For Questions: I to 7, choose the correct answer from the following list:
AScanning objective lens (4x) BLow-power objective lens (10x)C
High-power objective lens (40x) DOil immersion objective lens (100x)
Which is the shortest objective lens?

2 Which objective lens should you use when you begin to focus a
specimen? .A....

3. Which objective lens should be in position before you store a

 microscope?
4. Which objcctive lens will deliver thc highest amount of light?

Which objcctive lens requires immersion oil to fill up the space

betwcen the spccimen and the lens?
D

6 Which objective lens will still remain in focus when placed at

the longest working distance from the specimen?

7. When using an ocular lens with 10x magnification power, which

objective lens should be used to obtain the following actual
magnification?
a. 100 times of its diameter
b. 1000times of its diameter... D
Part B
1. Using a 40x objective lens, determine the length and width of acell
from apiece of cork tissue with approximately 20 cells in horizontal
position and 10 cells in vertical position.
vertical
Horizontal
20 cellS > 0.375 mm
lcell ? mm J cell ? mm

o.35 0-0 88 mm O0375 mn

2. Based on laboratory practices, what do you use to clean the

microscope lenses?
Lens tiSsue papen
3. While observinga moving microorganism under a microscope, you
found that the organism has moved out of the field of view to the right. In
order to keep observing the microorganism, which direction do you move
your slide (right/left)?

4. How do you adjust the slide when the specimen is out of the field of
view to the top?
Move the slide downuard
Part C
1.Complete the following sentences.
a. A microscope is called a compound microscope when it consists of
more than one set of Iens.
b. Condenser and iris diaphragm are useful to coordinate
intensity. f Gght.
Reference Solomon, E.f., Berg,k. R. and martin, D-w. CDDi8).Biology -(|" Ed). mark
Nelson tducation, Ltd. Canada.
Sequence lmark
Submission date Oy submiing hs report, 7cetty thet all the work in ttis report 3 marks
je my own wk
(not late)
Total /50