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NYC Agar Base: Composition

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21 views

NYC Agar Base: Composition

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Eurico
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© © All Rights Reserved
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NYC Agar Base M1348

NYC Agar Base is recommended for the selective isolation of gonococci .


Composition**
Ingredients Gms / Litre
Proteose peptone 15.000
Corn starch 1.000
Glucose 5.000
Sodium chloride 5.000
Dipotassium hydrogen phosphate 4.000
Potassium dihydrogen phosphate 1.000
Agar 20.000
Final pH ( at 25°C) 7.4±0.2
**Formula adjusted, standardized to suit performance parameters

Directions
Suspend 25.50 grams in 320 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at
15 lbs pressure (121°C) for 15 minutes. Avoid overheating. Cool to 45-50°C and add aseptically 100 ml of sedimented horse
blood cells and 60 ml of citrated horse plasma along with rehydrated contents of 1 vial of NYC Supplement (FD150) and 1
vial of Yeast Autolysate Supplement (FD027). Mix well and pour into sterile Petri plates.

Principle And Interpretation


NYC Agar Base was originally developed by Fauer, Weisburd and Wilson (1-3) at the New York City Department of Health
for selective isolation of pathogenic Neisseria species from clinical specimens. It consists of primarily a peptone-corn starch-
agar-base buffered with phosphates and supplemented with horse plasma, horse haemoglobin, dextrose, yeast autolysate and
antibiotics (1, 2). This medium is superior to other media generally employed for the isolation of Neisseria species (1, 4,
7). The transparent nature of the medium helps in studying the colonial types (9).
Proteose peptone, horse plasma, haemoglobin provide nutrients for the growth of N. gonorrhoeae and N. meningitidis
. Phosphate buffers the medium. The selective supplement added contains the antibiotics vancomycin, colistin, nystatin and
trimethoprim, to suppress the accompanying flora. Vancomycin is inhibitory for gram-positive bacteria. Colistin inhibits gram-
negative bacteria, including Pseudomonas species, while Proteus is inhibited by trimethoprim (8). The combination
of trimethoprim and colistin acts synergistically against gram-negative bacilli (6). Starch neutralizes the toxic metabolites
produced by Neisseria . The yeast autolysate supplement fulfils the CO2 requirements needed to enhance Neisseria
growth. Yeast contains oxaloacetic acid which is metabolized by gonococci to produce sufficient CO2 for growth of capnophilic
gonococci (5). Also, presence of yeast autolysate reduces the lag phase of growth of Neisseria , thus enhancing both size
and number of colonies. The specimen can be directly streaked on the medium to obtain maximum isolation.

Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 2.0% agar gel.
Colour and Clarity of Prepared medium
Yellow coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 5.1% w/v aqueous solution at 25°C. pH : 7.4±0.2
pH
7.20-7.60

Please refer disclaimer Overleaf.


HiMedia Laboratories Technical Data

Cultural Response
M1348: Cultural characteristics observed after in presence of 5-10% CO2 and 70% humidity with added sedimented horse
blood cells and citrated horse plasma along with rehydrated contents of 1 vial of NYC Supplement (FD150 and 1 vial of
Yeast Autolysate Supplement(FD027), after an incubation at 35-37°C for 40-48 hours.
Organism Inoculum Growth Recovery
(CFU)
Cultural Response
Haemophilus influenzae 50-100 good-luxuriant >=50%
ATCC 19418
Neisseria gonorrhoea ATCC 50-100 good-luxuriant >=50%
19424
Neisseria meningitidis ATCC 50-100 good-luxuriant >=50%
13090
Streptococcus pneumoniae 50-100 good-luxuriant >=50%
ATCC 6303
Streptococcus pyogenes 50-100 good-luxuriant >=50%
ATCC 19615
Pseudomonas aeruginosa 50-100 none-poor <=10%
ATCC 27853
Proteus mirabilis ATCC 50-100 none-poor <=10%
13883

Storage and Shelf Life


Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.

Reference
1. Fauer, Weisburd, Wilson and May, 1973, Health Lab. Sci., 10: 44.
2. Fauer, Weisburd and Wilson, 1973, Health Lab. Sci., 10: 55.
3. Fauer Y. C., Weisburd M. H. and Wilson M. E., 1973, Health Lab Sci., 10(2), 61.
4. Granato, Schneible-Smith and Weiner, 1981, J. Clin. Microbiol.13:963.
5. Lawton and Koch, 1982, J. Clin. Microbiol., 20: 905.
6. Simmons N. A., 1970, J. Clin. Pathol., 23, 757.
7. Griffin P. J. and Reider S. V., 1957, J. Biol. Med., 29, 613.
8. Murray P. R., Baron J. H., Pfaller M. A., Tenover F. C. and Yolken R. H. (Eds.), 1999, Manual of Clinical Microbiology,
7th Ed., American Society for Microbiology, Washington, D.C.
9. MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams
and Wilkins, Baltimore

Revision : 1 / 2011

Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147
1919 Email: [email protected]

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