2

WELCOME REMARK
From chairman of organizing committee
The 1st Mandala Waluya – International Conference on Pharmaceutical Science and Practice
(1st MW-ICPSP)

Assalamualaikum Wr Wb.

Dear Excellencies, Colleagues, Ladies and gentlemen.

First of all, let us pray and thank God for blessing and
granting us good health and the opportunity to gather in this venue
to attend this adorable and memorable moment of The 1st Mandala
Waluya – International Conference on Pharmaceutical Science
and Practice (1st MW-ICPSP). I express my deepest gratitude to
the plenary speakers and participants who are willing to join us in
this conference, we wish you and your family health and safety in
this COVID-19 pandemic. Moreover, i would like to say thank you
very much for the entire committee of the international conference
who had hardwork to prepare this seminar with good.
This Conference is not only a forum for researchers, but it can also be followed by students,
educators, observers, and practitioners from universities, research institutions, industry, and the
general public to exchange ideas and latest information in the field of pharmaceutical science and
practice.
The theme of this international seminar is “Advanced Drug Development and Discovery
from Natural Product”. This conference aims to discusses the latest advances in drug discovery
and development, which are in line with technological advances and scientific developments,
pharmaceutical drug discovery and development techniques as well as pharmaceutical practice are
increasingly moving to a wider level. This conference was attended by 6 plenary speakers, 40 oral
presentations, and 9 poster contributions from more than 500 participants, which cover various
topics in the field of pharmaceutical science, including Pharmaceutical Technology and
Pharmaceutics, Pharmaceutical Analysis, Medicinal Chemistry, Pharmacology and Toxicology,
Pharmaceutical Biology, Community Pharmacy, and Clinical Pharmacy.
Without the generous support provided by the Mandala Waluya University, this conference
would not have been possible at this scale. Many members of the organizing team worked very
hard to turn our initial visions for this virtual seminar into reality. Additionally, I would like to
warmly thank all the authors who, with their presentations and posters, generously contributed to
the lively exchange of scientific information that is so vital to the endurance of scientific
conferences of this kind.
Therefore, on behalf of the entire committee, I hope that this conference can be useful for
all. Your support will also make this a memorable and successful event.

Finally, i wish you enjoy the virtual conference.

Thank you very much

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 3

OPENING REMARKS
From Rector of Mandala Waluya University
The 1st Mandala Waluya International Conference on Pharmacuetical Science and Practice

First of all, I would like to convey my greetings and appreciations

to all of the invited speakers especially:
- Prof. Vivi Kasim (Chongqing University, China),
- Prof. Ibrahim Bin Jantan (National University of Malaysia,
Malaysia),
- Prof. apt. Zullies Ikawati, Ph.D. (Universitas Gadjah Mada,
Indonesia),
- Assoc. Prof. Rathapon Asasutjarit, Ph.D. (Thammasat
University, Thailand),
- Amir Jalil, PharmD., M.Phil.,PhD. (Bahauddin Zakaria
University, Pakistan).
- and Dwi Syah Fitra Ramadhan, M.S.Farm. (Mandala Waluya University, Indonesia)

Thank you for your participation in our event.

Greetings from Mandala Waluya University,

Ladies and gentlemen,

It is a great pleasure to welcome you to The 1st Mandala Waluya International Conference
on Pharmacuetical Science and Practice, organized by the department of Pharmacy, Faculty of
Science and technology, Mandala Waluya University.
I would like to start by wishing you and your families good health and safety in this difficult
time. As society begins to slowly recover from the COVID-19 pandemic, it is clear that COVID-
19 has reshaped the way we will live our lives for the foreseeable future. The world is facing many
predicaments that require joint hands from different stakeholders involved in a wide range of
actions for positive change. We also understand the importance of science, technology and
innovation in this challenging situation for transforming the world.
Due to the pandemic situation, The 1st Mandala Waluya International Conference on
Pharmacuetical Science and Practice will be held through a webinar. The first MW-ICPSP will
focus on “Advance Drug Development and Discovery from Natural Product” with many topics
including Biotechnology and Pharmaceutical Technology, Pharmacochemistry, Pharmaceutical
biology, Pharmacology, Clinical Pharmacy and Community Pharmacy.
This seminar will serve as a venue for researchers, professionals and students that have interests
in the area of pharmaceutical science and its related fields to build many collaborations for their
own research projects and will also enrich collaborations of the activity in education, research and
community service.
I hope this seminar will accomplish all its aims and earnestly desire that all participants
will be able to benefit from the presentations and discussions, and this seminar will enrich the

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 4

development of pharmaceutical science, not only in Indonesia but also in world wide. I would like
to thank the organizing committee for their tremendous efforts to make this program come to the
realisation. I hope all of the speakers and participants will gain many benefits and insightful
experiences. Hopefully, we will meet again in the next MW-ICPSP program.

Best Regards,
Dr. Ratna Umi Nurlila, S.Si., M.Sc.

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 5

WELCOME REMARKS
From Head Of Pharmacy Department, Mandala Waluya University

Assalamu alaikum Wr Wb.

Let us say "Alhamdulillah" at this wonderful event.

Praise be to God for providing graces, kindness, and health
for us to meet on this special day. First of all, I would like to
thank and welcome the honourable ones, the founders of the
Mandala Waluya University, the Rector of Mandala Waluya
University and the professors and lecturers from various
campuses who have taken the time to attend our conference
today. I'd also want to thank and welcome our presenters
who have accepted our offer to present at this virtual
conference. We also extend a warm welcome to the
participants of our first international conference. I'd want to
thank everyone who has joined us, both in this place and
virtually.
In this 1st Mandala Waluya – International Conference on Pharmaceutical Science and
Practice (1st MW-ICPSP) webinar with the theme "Advanced Drug Development and Discovery
from Natural Products," we as health workers and organizations involved in the pharmaceutical
sector believe that it is important to discuss the information related to the innovation and
application of technology in the development and discovery of new drugs.
Through this speech, I, as a representative of the pharmacy study program and the faculty
of science and technology at Mandala Waluya University, apologize for any shortcomings during
this activity, and hopefully this meeting will be beneficial to all of us.
Thanks you very much

Best Regards
apt. Wa Ode Yuliastri, S.Farm.,M.Si

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 6

TABLE OF CONTENTS

Welcome Remark From Chairman Of Organizing Committee ..................................................... 2

Opening Remarks From Rector Of Mandala Waluya University ................................................... 3
Welcome Remarks From Head Of Pharmacy Department, Mandala Waluya University ............ 5
Organizing Committee .................................................................................................................. 11
Conference Shedule ...................................................................................................................... 12
Time Schedule Paralel Session ..................................................................................................... 14
(Oral Presentation) ........................................................................................................................ 14
Acute Toxicity Test With BSLT Method And Antioxidant Activity With FRAP Method From
Ethanol Extract Of Teak Leaves (Tectona grandis L.) ................................................................. 16
Immunomodulatory Potency Of Hibiscus sabdariffa L. Fraction On Interleukin 12 (Il-12) Levels
In Balb/C Mice (Mus musculus..................................................................................................... 16
Characterization of Physical Properties and Stability Test of Formulation of Microemulsion from
Ethanol Extract of Moringa oleifera L. ........................................................................................ 25
Formulation and Antioxidant Activity of Cream Marine Sponge Extract (Axinella carteri) by
Using the DPPH Method (1.1-Diphenyl-2-Picrylhydrazyl).......................................................... 26
Delladari Mayefis, Hadifa Achria Permata Zain*, and Sri Hainil............................................. 26
Formulation and effectiveness testing of papaya .......................................................................... 27
Fruit meat liophilisate lip balm (Carica papaya) as solar screen ................................................. 27
Nielma Auliah1 , Nurjannah Bachri 2 , Suhrah Febrina Karim 1, Mirfaidah Nadjamuddin1 Jihan
Savira Kusuma Dewi3 ............................................................................................................... 27
Isolation of Secondary Metabolic Compounds Papaya Leaf Ethanol Extract (Carica papaya L )
As Antidiabetic ............................................................................................................................. 28
Sri Yolandari1, Irwan R.2 .......................................................................................................... 28
Phytochemical screening and xanthine oxidase inhibitory activity of Sinaphyl alcohol diacetate
and Stigmasterol Compounds from Etlingera rubroloba A.D Poulsen stem ............................... 29
Asriullah Jabbar1*, Muhammad Ilyas Y1.2, Fadhliyah Malik1, Sahidin1 , ................................ 29
Ika Puspita Sari3, Subagus Wahyuono4 .................................................................................... 29
Standardization and Formulation of Tawaloho Leaf Biscuits ...................................................... 30
From Southeast Sulawesi .............................................................................................................. 30
Suhikma Sofyan1, Eny Nurhikma2, Reymon2, Musdalipah*2 ................................................... 30
Powerful Natural Antioxidant From Rambutan Rapiah (Nephelium lappaceum) Rind ............... 31
Fahrauk Faramayuda*, Julia Ratnawati, Tita Hartyna Sutarna, Oktovia El Shara, Rizka Pratiwi
................................................................................................................................................... 31
Anti-inflammatory effects of Ethanol Extract of Cymbopogon nardus Herb on Rats Induced By
Carrageenan .................................................................................................................................. 32

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 7

Diana Puspita Sari1, Ririn Lispita Wulandari2*......................................................................... 32

Acute Toxicity Test of Ethanol Extract of sapodilla stem bark (Manilkara zapota (L.)) by BSLT
Methods......................................................................................................................................... 33
Ratna Widyasari 1, Deska Seria Ramadhanti 1, Reski Kurniawan 1, Yusril Izzamaulana 1, Dina
Yuspita Sari 1 ............................................................................................................................ 33
SPF Value and Physical Stability of Nutmeg Seed Oil Microemulsion with Tween 80 and
Propylene Glycol as Surfactan and Cosurfactant .......................................................................... 34
Ayu Shabrina* , Vironica Rizky Fauzia, Aza Zakiyatun Nida, Rizky Adityo, Muhammad
Imaamudin ................................................................................................................................ 34
Comparison of Cisplatin Drug Toxicity Test and Herbal Drug Combination of Bovine Serum
Albumin (BSA) and Folic Acid Nanoparticles as Anticancer Candidates ................................... 35
Ersalina Nidianti1, Rizka Amalia1, Nisrina Salsabila Firdaus1 ................................................. 35
Antioxidant Activity Test of Watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai)) Flesh
Methanolic Extract with ABTS and FRAP Method ..................................................................... 36
Nur Herlina Nasir1, Jastria Pusmarani1, Filmaharani1 .............................................................. 36
Antibacterial Activity Ethanol Extract of Chinese Petai ( Leucaena leucocephal (Lam.De
Wit),guava leaves ( Psidium guajava L.) and their combinations on the growth of Escherichia coli
Bacteria ......................................................................................................................................... 37
Suhaera1*,Yola Selvia1, Yufri aldi2 ........................................................................................... 37
Antibacterial activity of Meistera chinensis rhizome extract on Staphylococcus aureus and
Escherichia coli bacteria ............................................................................................................... 38
by agar diffusion method .............................................................................................................. 38
Karmilah, Reymon, Nur Saadah Daud, Yulianti Fauziah, Musdalipah* ................................... 38
Antioxidant Activity of Face Mist From Red Dragon Fruit Peel Extract (Hylocereus polyrhizus)
With DPPH Method ...................................................................................................................... 39
Sri Wahyuningsih*, A. Juaella Yustisi, Muhammad Asri SR1, Nurmala1 ............................... 39
Analgesic Activity of Dadap Serep (Erythrina subumbrans (Hassk.) Merr.) Leaf ...................... 40
Saru Noliqo Rangkuti, Arini Aprilliani ..................................................................................... 40
Formulation and Qualitatif Analysis of Sea Buckhthorn Oil Microemulsion using Isopropyl
Myristate ....................................................................................................................................... 41
Rapfika Setiyaningrum, Ayu Shabrina* ................................................................................... 41
Formulation and In Vitro SPF Value Test of Sea Buckhthorn Oil Microemulsion using Tween 80-
PEG 400 as Surfactant and Cosurfactant ...................................................................................... 42
Savna Dyah, Ayu Shabrina* ..................................................................................................... 42
Formulation and Antibacterial Activity of Hand Sanitizer Gel Containing Watermelon Peel
(Citrullus lanatus) Extract ............................................................................................................ 43
Deo Julian Hikmat1*, Filmaharani1, Yaya1, Nur Hatidjah Awaliyah1, Jastria Pusmarani2 ....... 43

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 8

Determination of Betamethasone and Dexchlorpheniramine Maleate in Tablet Dosage Form by

TLC Video Densitometry Method ................................................................................................ 44
Winasih Rachmawati*, Anggina Widya, Ivan Andriansyah .................................................... 44
Antioxidant Activity Assay Of Water Fraction, Ethyl Acetate Fraction And N-Hexan Fraction Of
Banana Peel (Musa paradisiaca Var. Sapientum) Extract............................................................ 45
Jastria Pusmarani1*, Ulfa2, Citra Dewi2.................................................................................... 45
In Silico Study of Xanthones, Alpha-Mangostin, And Gamma-Mangostin As Anti-Diabetic Drug
Candidate ...................................................................................................................................... 46
Rifa’atul Mahmudah1,2*, I Ketut Adnyana1, Elin Yulinah Sukandar1 ................................... 46
Acute Toxicity Test With BSLT Method And Antioxidant Activity With FRAP Method From
Ethanol Extract Of Teak Leaves (Tectona grandis L.) ................................................................. 47
Nuralifah1*, Parawansah 1,2, Waode Marianti1.......................................................................... 47
Antioxidant and Antihiperurisemia Activity Assay of Aegle marmelos L Leaves ....................... 48
Risky Juliansyah Putri*, Bai Athur Ridwan, Uyun Wardarini, Syamsiah Pawannei ................ 48
Phytochemical Constituent And In Vitro Cytotoxic Activity Of Hibiscus Sabdariffa L. Calyx
Fraction On Human Breast Cancer Cell Line MDA-MB-231 ...................................................... 49
W.O. Yuliastri1,2*, A. Diantini2, M. Ghozali3, I. Sahidin4, E.Julaeha, M. Isrul2 ...................... 49
Hair Growth Promoting Activity of Langir Bark (Albizia Saponaria Lour.) Ethanol Extract: In vivo
Assay ............................................................................................................................................. 50
Himaniarwati1,2, Muhammad Arba4, Yasmiwar Susilawati3, Resmi Mustarichie1 ................ 50
Immunomodulatory Potency Of Hibiscus sabdariffa L. Fraction On Interleukin 12 (Il-12) Levels
In Balb/C Mice (Mus musculus) ................................................................................................... 51
Wa Ode Yuliastri, Dyah Ayu Kusumo Winahyu...................................................................... 51
Formulation and antioxidant activity of lip balm ethanol extract paper flowers (Bougainvillea
spectabilis) .................................................................................................................................... 52
Immunomodulatory Potency Of Hibiscus Sabdariffa L. Fraction On Cluster Of Differentiation 8
(CD8) Levels In Balb/C Mice (Mus Musculus) ............................................................................ 53
Active Anti Alopecia Chemical Identification Of Merremia peltata Leaves And Computational
Study Toward Androgen Receptor Using Molecular Docking And Molecular Dynamic Simulation
....................................................................................................................................................... 54
Syawal Abdurrahman, Ruslin, Aliya Nur Hasanah, Resmi MustarichieError! Bookmark not
defined.
Potential Imunomodulator Fraction Of Rosella Flower (Hibiscus Sabdariffa L.) On Gamma
Interferon Levels (Inf-γ) In Balb/C Mice (Mus Musculus) ........................................................... 55
Wa Ode Yuliastri,2Niluh Eky Avisvani A.S ............................................................................. 55
The Potential Leaves Of Porang (Amorphophallus muerelli blume) As Antioxidant And
Antibacterial againts Escherichia coli and Salmonella typhi ....................................................... 56
Susanti Erikania, Vivi Rosalina ................................................................................................ 56

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 9

Pharmacy Departement, STIKES Bhakti Husada Mulia .............................................................. 56

In Silico Study Α-Mangostin Compounds As Inhibitors Of Glycogen Synthase Kinase 3β (Gsk
3β) Receptors In Breast Cancer .................................................................................................... 57
In silico study of compounds from crocus sativus l. Towards glycogen synthase kinase-3 beta (gsk-
3β) receptors as antidiabetic.......................................................................................................... 58
Dian Afriana,Citra Dewi, Dwi Syah Fitra Ramadhan ............................................................. 58
Potential Components of Gedi Leaves (Abelmoschus manihot (L.) Medik) as XO Enzyme
Inhibitors Causing Gout: an in silico Approach ........................................................................... 59
Faruk Jayanto Kelutur1,2*, Wa Ode Ida Fitriah3 ........................................................................ 59
Collagen Thickness and Density in BALB/c Mice Exposed to UVB Light After Using Siam Weeds
Cream (Chromolaena odorata L.) ................................................................................................ 60
Karlina Amir Tahir1, Upik A. Miskad2, Khairuddin Djawad3, Sartini4, Maulita Indrisari5, Arief
Budiyanto6, Ahmad Lalo7, Syamsuri Syakri1, Asrul Ismail1, Khaerani1 .................................. 60
In silico study phenolic compounds contained in rosella flower (hibiscus sabdariffa l.) As CTLA-
4 receptor inhibitors ...................................................................................................................... 61
Lingga Nurhayati, Wa Ode Yuliastri, Dwi Syah Fitra Ramadhan ........................................... 61
The Relationship Between Pharmaceutical Service Quality, Patient Satisfaction And Pharmacy
Installation Images At The Regional General Hospital Of Kendari City : A Comparative Study
Between Outpatient And Inpatient................................................................................................ 62
Juliana Baco, Sunarsih, Tasnim ................................................................................................ 62
Effectiveness Level of Vitamin C Use Against Kidney Damage in Mice (Mus musculus L) with
Injection Treatment ....................................................................................................................... 63
Dwintha Lestari1, Sindi Widia1 , Titian Daru Asmara Tugon2, Nanda Raudhatil Jannah3, Fauziah
Ningrum Syaputri4 .................................................................................................................... 63
Astaxanthin Gen Expression In Haematococcus pluvialis With NaCl Induction And High Light
Intensity......................................................................................................................................... 64
Soni Muhsinin1, Widhya Aligita1, Tina Rostinawati2, Jutti Levita3, ........................................ 64
The Analgesic and Antipyretic Activity Test of Syzigium cumini (L). Skeel Leaves Ethanol Extract
in Mice .......................................................................................................................................... 65
(Mus musclus) ............................................................................................................................... 65
Suwahyuni Mus*, Sitti Rahimah, Fadhillah Maryam ............................................................... 65
Determination of Total Phenolic Concentration of Ethanol Extract of Chrysophyllum cainito L.
Using UV-Vis Spectrophotometry................................................................................................ 66
Fadillah Maryam*, Imrawati, Suwahyuni Mus ........................................................................ 66
Physical and Antioxidant Stability of Lotion Nanoparticles of Soursop (Annona muricata L.)
Ethanolic Extract ........................................................................................................................... 67
Malinda Prihantini1, M Fatchur Rochman1, Anisa Rizki Amelia2, Tsabita Udzkiya Zulfa2, ... 67

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 10

Formulation Of Talas Leaf (Colocasia Esculenta) Emulgel As A Candidate Of Antibacterial

Causes Of Bulbs ............................................................................................................................ 68
Maria Ulfa*, Radia Reski, Muthiah Ridhayanah ...................................................................... 68
In Silico Study : The Blue Butterfly Pea Flower (Clitoria ternatea L.) Potency for Sars-Cov-2
Main Protease Inhihibor................................................................................................................ 69
Safira Yulita Fazadini ............................................................................................................... 69
Potential Antihyperlipidemic Activities From Purified Extract Of Lawsonia Inermis Leaves In
Hyperlipidemic Mice .................................................................................................................... 70
Mus Ifaya*, Melda Azis Sri Wulandari, Silviana Hasanuddin, Juliana Baco ........................... 70
Comparison of Extraction Methods to Antioxidant Activity and Determination of Total Flavonoid
and Phenolic Levels in Red Leeks (Allium ascalonicum .L) ........................................................ 71
Maria Ulfah1*, Dwi Yuni Mufarikhah1, Ranni Puji Astutik1, Mutmainnah1 ............................ 71

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 11

ORGANIZING COMMITTEE

STEERING COMMITTEE
Chancellor of Mandala Waluya University
Vice Chancellor of Mandala Waluya University
Dean of the Faculty of Science and Technology, Mandala Waluya University
Head of the Pharmacy Study Program, Mandala Waluya University

CHAIRMAN
Dwi Syahfitra Ramadhan, S.Farm., M.S.Farm

VICE CHAIRMAN
Rismayanti Fauziah, S.Farm., M.Si

SCIENTIFIC COMMITTEE
apt. Mus Ifaya, S.Farm.,M.Farm.
apt. Jastria Pusmarani, S.Farm., M.Sc
apt. Muhammad Isrul, S.Farm., M.Si
apt. Himaniarwati, S.Farm., M.Sc
apt. Nikeherpianti Lolok, S.Farm., M.Farm
apt. La Ode Muh. Andi Zulbayu, S.Farm., M.Sc
apt. Bai Athur Ridwan, S.Farm., M.Pharm.Sci
apt. Fatma Sari Siharis, S.Farm.,M.Si
apt. Citra Dewi,S.Farm., M.Farm

SECRETARIATE
apt. Silviana Hasanuddin, S.Farm., M.Farm
Selpirahmawati Saranani, S.Farm., M.Si
apt. Nurherlina Nasir, S.Farm., M.Pharm Sci

TREASURER
Nur Hatidjah Awaliyah Halid, S.Farm., M.Farm
Risky Juliansyah Putri, S.Farm., M.Si

SUPPORTING TEAM
apt. Juliana Baco, S.Farm., M.KM
apt. Dian Rahmaniar, S.Farm., M.KM
Titi Halfian., S.Kep., Ns
Intan Permatasari., S.S

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 12

CONFERENCE SHEDULE
The 1st Mandala Waluya International Conference on Pharmaceutical Sciences and
Practice (MW-ICPSP)
December 18, 2021

TIME (GMT
DURATION ACTIVITY
+8, WITA)

08.00-08.30 30 Minutes Registration and Coffee Break

Opening Ceremony
(1) Singing national anthem of Indonesia – “Indonesia Raya” and singing MARS of
5 Minutes
Universitas Mandala Waluya
08.30-09.00
10 Minutes (2) Traditional Dance
(3) Welcoming Remarks by Rector of Universitas Mandala Waluya : Dr. Ratna
15 Minutes
Umi Nurlila, S. Si., M. Sc.
Plenary Lectures (Moderator: Dr. Rer. Nat. apt. Adryan Fristiohadi, M. Sc.)
Plenary Speaker 1
Prof. Ibrahim Bin Jantan
09.00-09.25 25 Minutes Department of Natural Product Chemistry,
National University of Malaysia, Malaysia
Topic: Natural products research: scientific interests and advances, research
approach, strategy and future directions
Plenary Speaker 2
Rathapon Asasutjarit, Ph. D
09.25-09.50 25 Minutes Department of Pharmaceutical Tecnology, Thammasat University, Thailand
Topic: Development of Pharmaceutical Ophthalmic Product by Using
Bioflavonoid from Natural Product
Plenary Speaker 3
Aamir Jalil, PharmD., M. Phil., Ph.D.
09.50-10.15 25 Minutes Department of Pharmaceutical Tecnology, Bahauddin Zakaria University,
Multan, Pakistan
Topic: Self emulsifying drug delivery systems A game changing approach in drug
delivery.
10.15-10.30 15 Minutes Discussion
Plenary Lectures (Moderator: apt. Edwin J.B., S. Farm., M. Eng.)
Plenary Speaker 1
Prof. apt. Zullies Ikawati, Ph. D
10.30-10.55 25 Minutes Department of Clinical Pharmacy and Pharmacology, Universitas Gadjah Mada,
Indonesia
Topic: Evidence-based phytotherapy

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 13

Plenary Speaker 2
Prof. Vivi Kasim
10.55-11.20 25 Minutes
Molecular Biomedical Laboratory, Chongqing, China
Topic: Drug Discovery: Optimizing the Natural Products
Plenary Speaker 3
Dwi Syah Fitra Ramadhan, . M. S. Farm.
11.20-11.45 25 Minutes Department of Medicinal Chemistry Pharmacy, Universitas Mandala Waluya,
Kendari, Indonesia
Topic: Computational Drug Discovery of Natural Products
11.45-12.00 15 Minutes Discussion
12.00-13.00 60 Minutes Lunch Break
Oral Presentation
100 Room A
13.00-14.50
Minutes Moderator: Selpirahmawati Saranani, S. Farm., M. Si.
100 Room B
13.00-14.50
Minutes Moderator: apt. Edwin Jeika Bunggulawa, S. Farm., M. Eng.
100 Room C
13.00-14.50
Minutes Moderator: apt. Jastria Pusmarani, S. Farm., M. Sc
100 Room D
13.00-14.50
Minutes Moderator: Wa Ode Ida Fitriah, S. Farm., M. Farm.
Closing
Dean of Faculty of Science and Technology Universitas Mandala Waluya: Dr. Sri
14.50-15.05 15 minutes
Anggarini Rasyid, S. Si., M. Si.

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 14

TIME SCHEDULE PARALEL SESSION

(ORAL PRESENTATION)

Room A
Moderator : Selpirahmawati Saranani, S.Farm.,M.Si

Time (GMT
Code Presenter Name Title
+8, WITA)
Characterization of Physical Properties and
Stability Test of Formulation of
13.00 – 13.00 OP 01 Benni Iskandar
Microemulsion from Ethanol Extract of
Moringa oleifera L.
Formulation and Antioxidant Activity of
Cream Marine Sponge Extract (Axinella
13.10 – 13.20 OP 02 Delladari Mayefis
carteri) by Using the DPPH Method (1.1-
Diphenyl-2-Picrylhydrazyl)
Formulation And Effectiveness Testing Of
13.20 – 13.30 OP 03 Nielma Auliah Papaya Fruit Meat Liophilisate Lip Balm
(Carica papaya) As Solar Screen
Isolation of Secondary Metabolic
13.30 – 13.40 OP 04 Sri Yolandari Compounds Papaya Leaf Ethanol Extract
(Carica papaya L) As Antidiabetic
Phytochemical screening and xanthine
oxidase inhibitory activity of Sinaphyl
Dr. apt. Asriullah Jabbar, SH,
13.40 – 13.50 OP 05 alcohol diacetate and Stigmasterol
S.Si.,MPH
Compounds from Etlingera rubroloba A.D
Poulsen stem
Standardization and Formulation of
13.50 – 14.00 OP 06 Musdalipah Tawaloho Leaf Biscuits From Southeast
Sulawesi
Powerful Natural Antioxidant From
14.00 – 14.10 OP 07 Fahrauk Faramayuda Rambutan Rapiah (Nephelium lappaceum)
Rind
Anti-inflammatory effects of Ethanol
14.10 – 14.20 OP 08 Ririn Lispita Wulandari Extract of Cymbopogon nardus Herb on
Rats Induced By Carrageenan
Acute Toxicity Test of Ethanol Extract of
apt. Ratna Widyasari.,
14.20 – 14.30 OP 09 sapodilla stem bark (Manilkara zapota (L.))
M.Farm-Klin
by BSLT Methods
SPF Value and Physical Stability of Nutmeg
Seed Oil Microemulsion with Tween 80 and
14.30 – 14.40 OP 10 Ayu Shabrina
Propylene Glycol as Surfactan and
Cosurfactant

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 15

Room B
Moderator : Apt. Edwin Jeika B. S.Farm., M.Eng

Time (GMT
Code Presenter Name Title
+8, WITA)
Comparison of Cisplatin Drug Toxicity Test
and Herbal Drug Combination of Bovine
13.00 – 13.00 OP 11 Ersalina Nidianti
Serum Albumin (BSA) and Folic Acid
Nanoparticles as Anticancer Candidates
Antioxidant Activity of Methanolic Extract
Flesh Watermelon (Citrullus lanatus
13.10 – 13.20 OP 12 Filmaharani
(Thunb.) Matsum. & Nakai)) by ABTS and
FRAP Method
Antibacterial Activity Ethanol Extract of
Chinese Petai ( Leucaena leucocephal
13.20 – 13.30 OP 13 Suhaera (Lam.De Wit),guava leaves ( Psidium
guajava L.) and their combinations on the
growth of Escherichia coli Bacteria
Antibacterial activity of Meistera chinensis
rhizome extract on Staphylococcus aureus
13.30 – 13.40 OP 14 Karmilah, S. Farm.,M.Si
and Eschericia coli bacteria by agar
diffusion method
Antioxidant Activity of Face Mist From Red
13.40 – 13.50 OP 15 apt. Sri Wahyuningsih, M.Si. Dragon Fruit Peel Extract (Hylocereus
polyrhizus) With DPPH Method
Analgesic Activity of Dadap Serep
13.50 – 14.00 OP 16 Saru Noliqo Rangkuti
(Erythrina subumbrans (Hassk.) Merr.) Leaf
Formulation and Qualitatif Analysis of Sea
14.00 – 14.10 OP 17 Rapfika Setiyaningrum Buckhthorn Oil Microemulsion using
Isopropyl Myristate
Formulation and In Vitro SPF Value
14.10 – 14.20 OP 18 Savna Dyah Nur Safitri
Test of Sea Buckhthorn Oil
Microemulsion using Tween 80-PEG
400 as Surfactant and Cosurfactant
Formulation and Antibacterial Activity of
14.20 – 14.30 OP 19 Deo Julian Hikmat Hand Sanitizer Gel Containing Watermelon
Peel (Citrullus lanatus) Extract
Determination of Betamethasone and
Dexchlorpheniramine Maleate in Tablet
14.30 – 14.40 OP 20 apt. Winasih Rachmawati, M.Si.
Dosage Form by TLC Video Densitometry
Method

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 16

Room C
Moderator : apt. Jastria Pusmarani, S. Farm., M. sc

Time (GMT
Code Presenter Name Title
+8, WITA)
Antioxidant Activity Assay Of Water
apt. Jastria Pusmarani, S. Fraction, Ethyl Acetate Fraction And N-
13.00 – 13.00 OP 21
Farm., M. sc Hexan Fraction Of Banana Peel (Musa
paradisiaca Var. Sapientum) Extract
In Silico Study of Xanthones, Alpha-
13.10 – 13.20 OP 22 Rifa'atul Mahmudah Mangostin, And Gamma-Mangostin As
Anti-Diabetic Drug Candidate
Acute Toxicity Test With BSLT Method
And Antioxidant Activity With FRAP
Nuralifah,S.Farm.,M.Kes.,Apt
13.20 – 13.30 OP 23 Method From Ethanol Extract Of Teak
Leaves (Tectona grandis L.)

Risky Juliansyah Putri, antioxidant and antihiperurisemia assay of

13.30 – 13.40 OP 24
S.Si.,M.Si aegle mormales L leaves
Phytochemical Constituent and In vitro
Cytotoxic activity of Hibiscus Sabdariffa L
13.40 – 13.50 OP 25 Wa Ode Yuliastri
Calyx Fraction on Human Breast Cancer
Cell Line MDA-MB-231
Hair Growth Promoting Activity Of Langir
13.50 – 14.00 OP 26 Himaniarwati Bark (Albizia saponaria Lour.) Ethanol
Extract: In Vivo Assay
Immunomodulatory Potency Of Hibiscus
sabdariffa L. Fraction On Interleukin 12
14.00 – 14.10 OP 27 Dyah ayu Kusumo Winahyu
(Il-12) Levels In Balb/C Mice (Mus
musculus)
Formulation and antioxidant activity of lip
balm ethanol extract paper flowers
14.10 – 14.20 OP28 Nur Hatidjah Awaliyah Halid
(Bougainvillea spectabilis)

Immunomodulatory Potency Of Hibiscus

14.20 – 14.30 OP 29 Nur Apsa Savitri Sabdariffa L. Fraction On Cluster Of
Differentiation 8 (CD8) Levels In Balb/C
Mice (Mus Musculus)
Active Anti Alopecia Chemical
Identification Of Merremia peltata Leaves
14.30 – 14.40 OP 30 Syawal Abdurrahman And Computational Study Toward
Androgen Receptor Using Molecular
Docking And Molecular Dynamic
Simulation

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 17

Room D
Moderator : Wa Ode Ida Fitriah, S. Farm., M. Farm.

Time (GMT
Code Presenter Name Title
+8, WITA)
Potential Imunomodulator Fraction Of
Rosella Flower (Hibiscus Sabdariffa L.)
13.00 – 13.00 OP 32 Niluh Eky Avisvani A.S
On Gamma Interferon Levels (Inf -
Gamma) In Balb/C Mice (Mus Musculus)
The Potential Leaves Of Porang
(Amorphophallus muerelli blume) As
13.10 – 13.20 OP 33 Susanti Erikania
Antioxidant And Antibacterial againts
Escherichia coli and Salmonella typhi
In Silico Study Α-Mangostin Compounds
As Inhibitors Of Glycogen Synthase
13.20 – 13.30 OP 34 Rizky Dwi Handayani
Kinase 3β (Gsk 3β) Receptors In Breast
Cancer
In silico study of compounds from crocus
sativus l. Towards glycogen synthase
13.30 – 13.40 OP 35 Dian Afriana
kinase-3 beta (gsk-3β) receptors as
antidiabetic
Potential Components of Gedi Leaves
13.40 – 13.50 OP 36 Faruk Jayanto Kelutur (Abelmoschus manihot (L.) Medik) as XO
Enzyme Inhibitors Causing Gout: an in
silico Approach
Collagen Thickness and Density in
13.50 – 14.00 OP 37 Karlina Amir Tahir BALB/c Mice Exposed to UVB Light
After Using Siam Weeds Cream
(Chromolaena odorata L.)
In silico study phenolic compounds
contained in rosella flower (hibiscus
14.00 – 14.10 OP 38 Lingga Nurhayati
sabdariffa l.) As CTLA-4 receptor
inhibitors
The Relationship Between Pharmaceutical
Service Quality, Patient Satisfaction And
14.10 – 14.20 OP 39 Juliana Baco Pharmacy Installation Images At The
Regional General Hospital Of Kendari City
: A Comparative Study Between Outpatient
And Inpatient
14.20 – 14.30 OP 40

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 18

BIOGRAPHY PRESENTER
Prof. Dr. Ibrahim Jantan

Dr. Ibrahim Jantan graduated from University of

Mansoura, Egypt with BPharm (Hons) degree in 1981,
obtained his MSc in Medicinal Chemistry from
University of Minnesota, USA in 1985 and his PhD
degree in Natural Products Chemistry from the
University of Malaya in 1993.
He is currently an honorary professor at Institute
of Systems Biology (INBIOSIS), Universiti
Kebangsaan Malaysia (UKM). Prior to his present
position, he was a professor at Taylor’s University,
Malaysia, from 2018-2020. He was with the Faculty of
Pharmacy UKM from 1996-2018 and was the
founding Dean from 2008 to 2015.
Dr. Ibrahim started his research career at the
Forest Research Institute of Malaysia (FRIM) in 1986. He was the President of the
Malaysian Natural Products Society (2010-2020), member of the Malaysian Herbal
Council, board member of Asian Association School of Pharmacy and Malaysian Focal
Point for Medicinal Plants of Malaysia for Indian Ocean Rim Association. Recently, he
was appointed as Research Advisor of Nan Yang Academy of Sciences (Singapore) and
Scientific Advisor of Human Life Advancement Foundation, Malaysia.
He has more than 34 years of research experiences in natural products and medicinal
chemistry and pharmacological activities of natural products. His research interests are
identification of natural bioactive compounds, their derivatives and analogues as chemical
leads for specific therapeutic efficacy (cardiovascular protective, immunomodulatory,
anti-inflammatory, PAF antagonist & antimicrobial activities) leading to the development
of new drug candidates against complex and challenging drug targets prior to clinical
trials, and herbal research to develop high quality, safe and effective herbal products.
He has published 206 papers in ISI-indexed journals, 73 papers in proceedings, 2
books and 5 chapters in books. His Scopus H-index is 34. He was listed as World Top 2%
Influencing Researcher 2019 and 2020, and career long impact based on SCOPUS Citation
Impact. He has been invited to deliver many keynote, plenary and invited lectures in local
and international conferences. He is a reviewer of manuscripts in ISI-indexed journals
such as Phytomedicine, Phytotherapy Research, Phytochemistry, Journal of
Ethnopharmacology, Pharmaceutical Biology, Drug Design Development and Therapy,
Frontiers in Pharmacology, BMC Complementary and Alternative Medicines. He was
recipients of many awards including Medicine Srinakharinwirot University (MEDSWU)
Honorable Award 2010, Thailand, Prof. Dr. A. Hisham Endowment Award, India,
Distinguished Researcher Award (Tokoh Penyelidik) 2013, UKM Medical Center, and
the Darjah Setia Bakti Negeri Sembilan (DBNS) which carries the title Dato’.

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 19

Biography
Rathapon Asasutjarit, Ph.D

Asasutjarit studied at Faculty of Pharmaceutical Sciences,

Chulalongkorn University, Bangkok, Thailand as bachelor degree,
continue studied at Faculty of Pharmaceutical Sciences,
Chulalongkorn University, Bangkok, Thailand, received Ph. D in
Ph. D. in Pharmaceutics at Faculty of Pharmaceutical Sciences,
Chulalongkorn University, Bangkok, Thailand. He is an Associate
professor pharmaceutical technology at Department of
Pharmaceutical Sciences, Faculty of Pharmacy, Thammasat
University, Pathumthani, Thailand. His current research are
application of polymers and surfactants as drug and cosmetic
delivery systems, nanotechnology for drug delivery and skin and
ophthalmic drug delivery.

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 20

Biography
Aamir Jalil, Ph.D

Aamir Jalil received philosophy of Doctor (PhD.) in

Pharmaceutical Sciences from Institute of Pharmacy, University
of Innsbruck, Austria. He received his M.Phil. Pharmaceutics from
Faculty of Pharmacy, Bahaudin Zakariya University, Multan,
Pakistan. He studied at Doctor of Pharmacy (PHARM-D) Faculty
of Pharmacy, Gomal University, Dera Ismail Khan, Pakistan. He
was teached and supervised six Master Thesis at University of
Innsbruck, Institute of Pharmaceutical Technology, Innsbruck
Austria. He is an assistant Professor, department of Pharmaceutics,
Faculty of Pharmacy, Bahauddin Zakaria University, Multan,
Pakistan.
He was received several rewards, including national bachelors scholarship award for medical
health studies (2006 – 2011), overseas Scholarship for PhD (HEC Pakistan and OeAD Austria
(2016-2020), national bachelors scholarship award for medical health studies (2006 – 2011),
overseas scholarship for PhD (HEC Pakistan and OeAD Austria (2016-2020), research grant of
PKR 176000/- as principal investigator from Comsats University and nomination for ACS
infectious Disease Young Investigator Award 2020.
His current interest research include designing of drug carrier systems for oral delivery of
therapeutics, product development from research bench to market, , application of nanotechnology
fundamentals to develop nano based drug vehicles, self-emulsifying drug delivery systems to
develop oral formulations of water insoluble and peptide/protein drugs, zeta potential changing
systems for delivery of genes and peptide drugs, designing, synthesis and testing of thiolated
polymers for the purpose of mucosal drug delivery, synthesis and modification of excipients for
developing cosmetics and for aesthetic surgeries., toxicology studies and safety screening of the
chemicals, excipients and newly designed formulation using in-vitro cell cultures and freshly
excised tissues, developing excipients for regenerative medicine to have 3D cell culture in order
to provide in-vivo stability and proliferation, analysis and screening of drugs using different
analytical chemistry procedures and tools, designing of hydrogel systems for artificial organs such
as knee and spinal cartilage and microbiological testing procedures for drug delivery systems and
active therapeutic ingredients.

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 21

Biography
Prof. Zullies Ikawati

Prof. Dr. Zullies Ikawati studied bachelor degree of

Pharmacy at faculty of pharmacy, Universitas Gadjah
Mada, Indonesia. Her received Doctor of Philosophy
(Ph.D) in Pharmacology from Ehime University School of
Medicine, Japan. Her current interest research studied in
pharmacology and clinical pharmacy. She was published
result of research at National and International Journal. She
is a speaker or narasumber at several scientific and
phamaceutical seminar in Indonesia and reviewer at
several scientific national and international journal.
She received several awards, including second winner
for oral presentation in Regional Conference on Molecular
Medicine di Malaysia by Institute for Research on
Molecular Medicine USM, award the best presenter in pharmacology and toxicology at Scientific
Congress Indonesian Pharmacists Association in Makassar, Universitas Gadjah Mada achievers as
the best RPKPS developers, Head of Study Program Universitas Gadjah Mada achievement, at the
Universitas Gadjah Mada Achievement Human Award. She has published 11 books (2010-2019).
He is a member of several academic including member of Indonesian Pharmacists Association
Center, Indonesia, PB IKAFI, and vice chairman of KAGAMA Pharmacist.

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 22

Biography
Prof. Vivi Kasim

Vivi Kasim completed her undergraduate study and

received her Bachelor in Engineering from Department
of Chemistry and Biotechnology, Faculty of
Engineering, University of Tokyo. She received her
Master degree in 2004 from the Department of
Chemistry and Biotechnology, School of Engineering,
University of Tokyo. She accomplished her doctoral
program as a JSPS Special Researcher and received
her Doctor of Philosophy (Ph.D.) from Department of
Chemistry and Biotechnology, Faculty of Engineering,
University of Tokyo. She did her post-doctoral training
in Institute of Molecular and Cellular Biosciences,
University of Tokyo as a JSPS Fellow, and worked as a
Research Residence in the Research Institute, National
Cancer Center, Tokyo, Japan. In 2013, she joined Chongqing University, China. Currently, she is
a Full Professor in Chongqing University, and is the Principal Investigator of Lab. of Molecular
Biomedicines, College of Bioengineering, Chongqing University. She is a “High-level talent” of
the Municipal of Chongqing and is the group leader of the “Teaching group for International Post-
graduate Students” in College of Bioengineering, Chongqing University. Dr. Kasim is fluent in
English, Japanese, Indonesian, and Chinese.
Dr. Kasim’s research focuses on basic and applicative researches of tumor initiation and
progression, especially on cell cycle regulation, mitotic errors, tumor cells microenvironment,
tumor angiogenesis, tumor initiating cells, and tumor cells metabolic reprogramming.
Furthermore, she is also focusing on the development of small molecule drugs from natural
products for treating cancer and hind-limb ischemic disease. She has received more than 10 funds
from the National Natural Science Foundation, Natural Science Foundation of Chongqing, as well
as from the Chongqing University. She has published more than 30 publications in Nature, Science
Advances, PNAS, Cancer Research, etc., and has co-authored several books.

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 23

Biography
Dwi Syah Fitra Ramadhan

Dwi Syah Fitra Ramadhan studied bachelor degree of pharmacy from

universitas Halu Oleo, Kendari, Indonesia. His received Master of Science
Pharmacy from Institute Technology Bandung, Indonesia. His research
focuses on basic and applicative researches of medicinal chemistry specific
in computer aided drug design. His Research interest in modelling of mutan
or ressietence cancer of EGFR and discovery of potential allosteric inhibitor
true computational simulation. And now, in Universitas Mandala Waluya
his research focuses on discovery of marine natural products true
computational simulation against infectious diseases.

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 24

ABSTRACT

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 25

OP 01

Characterization of Physical Properties and Stability Test of

Formulation of Microemulsion from Ethanol Extract of
Moringa oleifera L.
Benni Iskandar1,2*, Alifya Octary1, Noveri Rahmawati1, Meircurius Dwi Condro Surboyo3,
Leny4, Ching-Kuo Lee1
1
School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan
2
Sekolah Tinggi Ilmu Farmasi Riau, Riau, Indonesia
3
Department of Oral Medicinie, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
4
Faculty of Pharmacy and Health, Institut Kesehatan Helvetia, Medan, Indonesia

Corresponding author: [email protected]

Moringa oleifera L. are known to have antioxidant activity because of its flavonoid compound.
This component will inhibit free radicals and very potent to be used as anti-aging. The purpose of
this study was to formulate the ethanol extract of Moringa oleifera L. into a stable microemulsion
and shows good physical characteristics. By decreasing the size of particles into a microemulsion,
it will accelerate the absorption rate into the skin. The formulation of the microemulsion
formulation were made by using ethanol extract of Moringa oleifera L. with concentrations of 0%,
3%, 6% and 9%. Microemulsion was formed by adding tween 20, hydrogenated castor oil,
propylene glycol, sodium EDTA, sodium metabisulfite, aquabidest, and oleum rosae. The
preparations were then being examined on its organoleptic, particle size, pH value, viscosity,
density, emulsion type, stability, and antioxidant activity test.
The results showed that all preparations were stable for 8th weeks storage, the
microemulsion spray had an average particle size of 6,3 to 6,9 m , the pH range was 4.4-6.4,
viscosity of the preparation was 107,3 to 132,6 cPs, the density was 1,019-1,042 g/ml and the IC50
value was 135, 639 g/ mL which shows moderate antioxidant activity. From these results, it is
known that the ethanol extract of Moringa leaves can be formulated as a microemulsion spray
preparation with good characteristics and stable during storage.

Keywords: Moringa oleifera L., antioxidant, anti-aging, microemulsion

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 26

OP 02

Formulation and Antioxidant Activity of Cream Marine

Sponge Extract (Axinella carteri) by Using the DPPH Method
(1.1-Diphenyl-2-Picrylhydrazyl)
Delladari Mayefis, Hadifa Achria Permata Zain*, and Sri Hainil
Faculty Of Pharmacy, Mitra Bunda Health Institute, Batam City, Indonesia

Corresponding author: [email protected]

Sponges are one of the marine biotas that has bioactive potential that is useful as an antioxidant to
prevent free radicals. This study aims to find out of preparations antioxidant activity cream
containing marine sponge extracts (Axinella carteri) against DPPH and how much concentration
of a cream extract marine sponge (Axinella carteri) that has the highest activity rate as antioxidant.
The marine sponges are extracted by maceration method using methanol solvents then made
preparations in cream form using variations in extracts concentrations of 5%, 7,5% and 10%.
Furthermore, the cream formulation of marine sponge extract was tested for the preliminary cream
testing parameters like organoleptic test, homogeneity, spreadability, pH, viscosity and cream type
test. Antioxidant activity determined with a method of DPPH that expressed in IC50 (Half-maximal
inhibitory concentration). The result of IC50 obtained in formula I is 539,74 ppm (very low),
formula II is 398,00 ppm (low) and formula III is 244,14 ppm (medium). Meanwhile, for the
positive control vitamin C has an IC50 value of 5.02 ppm (very active). Formula III is the best
formula which has medium rate of antioxidant activity.

Keywords: Marine sponge, cream, antioxidant, DPPH

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 27

OP 03

Formulation and effectiveness testing of papaya

Fruit meat liophilisate lip balm (Carica papaya) as solar
screen
Nielma Auliah1 , Nurjannah Bachri 2 , Suhrah Febrina Karim 1, Mirfaidah Nadjamuddin1 Jihan
Savira Kusuma Dewi3
1
Pharmacy Faculty, Program Studi D3 Farmasi, Megarezky University, Makassar, Indonesia
2
Pharmacy Faculty i, Program Studi S1 Farmasi, Sekolah Tinggi Ilmu Kesehatan Tarumanegara, Indonesia
3
Pharmacy Faculty, Program Studi S1 Farmasi, Megarezky University, Makassar, Indonesia

*Corresponding author E-mail : [email protected]

This study aims to examine the effect of lip balm lyophilized papaya (Carica papaya) flesh as
sunscreen. Papaya fruit (Carica papaya) has flavonoid compounds, tannins, saponins, vitamin C,
vitamin E and vitamin A which function as antioxidants in lip balm preparations to protect lips
from free radicals caused by sunlight (UV). Research has been conducted with the aim to knowing
the effectiveness of lyophilized sunscreen of papaya flesh (Carica papaya) which is formulated
in the form of Lip balm based on the measurement of the SPF (Sun protecting factor) value. The
research methods is carried out experimentally, this method uses Freeze drying, Freeze drying is
a drying tool through which the transfer process occurs indirectly, namely between the material
to be dried (wet material) and the heating medium. Lip balm preparations were made using papaya
(Carica papaya) flesh lyophilisate with various concentrations, namely negative control (0%),
Formula 1 (5%), Formula 2 (10%), and Formula 3 (15%). Parameters observed in this study
include organoleptic test, homogeneity test, melting point test, pH test, Cycling test, Skin analyzer
test, irritation test, preference test, and SPF test. The Results showed that based on statistical
analysis of the SPF value using One Way Anova with the SPSS 25.0 program, it was found that
variations in the concentration of lyophilisate in papaya pulp (Carica papaya) had a significant
effect (p < 0.05) on the SPF value of Lip balm preparations with negative control results. (0%)
3.23 (Minimum protection), Formula 1 (5%) 11.13 (Maximum protection), Formula 2 (10%)
12.37 (Maximum protection), and Formula 3 (15%) 13.37 (maximum protection). So it can be
concluded that the highest SPF value in Formula 3 (15%) is 13.37 with maximum protection.

Keywords : Papaya Lyophilisate, Frezze drying, Lip balm, SPF (Sun protecting factor)

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 28

OP 04

Isolation of Secondary Metabolic Compounds Papaya Leaf

Ethanol Extract (Carica papaya L ) As Antidiabetic
Sri Yolandari1, Irwan R.2
1
Pharmacy Department, Politeknik Baubau, Baubau, Indonesia.
2
Pharmacy Department, Politeknik Baubau, Baubau, Indonesia.

*Corresponding author. Email: [email protected]

Diabetes mellitus is the most common metabolic syndrome worldwide with an incidence of 1-8%..
In recent years, plant secondary metabolites have been widely studied as sources of medicinal
agents. Treatment of diabetes mellitus can be done medically with modern drugs and injections,
but because of the high cost of medical treatment this is sometimes difficult. Diabetes mellitus can
also be overcome with natural remedies by utilizing medicinal plants. Therefore, traditional
medicine with medicinal plants is an alternative step to overcome it. So in this study, the extraction
and isolation of compounds on papaya leaves will be carried out to obtain secondary metabolites
as new antidiabetic compounds. In this study, tests were carried out to determine the secondary
metabolites produced. The tests that will be carried out include maceration of papaya leaves,
identification of flavonoids, separation of flavonoid compounds, purification using preparative
thin layer chromatography (TLC), isolate spectroscopy. The results of the research in this study
were that there were flavonoids in the ethanol extract of papaya leaves, and flavonoid stains at
TLC with a wavelength of 365 nm were clearly visible, and there were 3 bands indicating that
there were flavonoids in the ethanol extract of papaya leaves at a wavelength of 200. nm - 600 nm
using a spectrophotometer
Based on the results of research that has been carried out on the ethanol extract of papaya leaves,
it is known that the ethanol extract of papaya leaves contains flavonoid compounds as evidenced
by the formation of positive results in the initial identification of flavonoids, then column
chromatography test until the spectrophotometric test contains flavonoid compounds, so one The
compound structure contained in the ethanol extract of papaya leaves that has the potential to be
an antidiabetic is the structure of flavonoid compounds in general.

Key words: Papaya leaves, isolation, Antidiabetic

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 29

OP 05

Phytochemical screening and xanthine oxidase inhibitory

activity of Sinaphyl alcohol diacetate and Stigmasterol
Compounds from Etlingera rubroloba A.D Poulsen stem
Asriullah Jabbar1*, Muhammad Ilyas Y1.2, Fadhliyah Malik1, Sahidin1 ,
Ika Puspita Sari3, Subagus Wahyuono4
1
Department of Pharmacy, Faculty of Pharmacy, Universitas Halu Oleo, Kendari, 93232, Indonesia
2
Politeknik Bina Husada Kendari, 93117, Indonesia
3
Department of Pharmacology and clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada,
Yogyakarta, 55281 Indonesia
4
Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta,
55281 Indonesia.
*Corresponding author: [email protected]

Etlingera rubroloba (Blume) AD Poulsen is a plant of the genus Etlingera found in Southeast
Sulawesi. This plant is widely used in local communities to relieve joint pain and increase stamina.
This plant has been isolated and identified so that it gets the compounds Sinapyl alcohol diacetate
and Stigmasterol. E. rubroloba and these two compounds have never been reported for
phytochemical screening tests and evaluation of Xanthine Oxidase (XO) inhibitory activity in
vitro. In this study, phytochemical screening tests were carried out using color reagents and were
positive for flavonoid compounds, alkaloids, tannins, saponins, and terpenoids. While the
inhibitory activity (IC50) of xanthine oxidase (XO) against Sinaphyl alcohol diacetate was 150.00
µg/mL ±0.278 µg/mL, stigmasterol 97.121 µg/mL ± 0.142 µg/mL and Allopurinol 39.507 µg/mL
± 0.156 g/ml as positive controls. This research is a reference in the development of drugs that are
sourced from nature.

Keywords: E. rubroloba, sinaphyl alcohol diacetate, stigmasterol, xantin oksidase

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 30

OP 06

Standardization and Formulation of Tawaloho Leaf Biscuits

From Southeast Sulawesi

Suhikma Sofyan1, Eny Nurhikma2, Reymon2, Musdalipah*2

Departement of Pharmacy, Politeknik Bina Husada, Kendari, Indonesia
Departement of Pharmacy, Politeknik Bina Husada, Kendari, Indonesia
*Corresponding author. Email: [email protected]

Tawaloho leaves or commonly called forest kedondong leaves are herbal plants that are efficacious
as antioxidants, anti-inflammatories, antidementia, antipyretics, analgesics, hypoglycemics, and
antimicrobials. In Southeast Sulawesi, forest kedondong leaves or better known as tawaloho leaves
are used as a complementary seasoning for foods in the Tolaki tribe of Southeast Sulawesi. This
study aims to test the standardization of extracts and formulations of tawaloho leaf biscuits as
healthy food. The sample of tawaloho leaf was extracted using methanol as solvent and
concentrated in a vacuum rotary evaporator. Extract standardization was analyzed using specific
and non-specific parameters. Biscuit formulations were made using tawaloho leaf Simplicia
powder with varying concentrations of 100 mg, 300 mg, 500 mg and physical evaluation of the
biscuit preparation was carried out. The results showed the standardization test with specific
parameters of water-soluble compounds (3.52%), ethanol-soluble (3.78%), phytochemical
screening of tawaloho leaves showed the presence of secondary metabolites of alkaloids, saponins,
steroids, flavonoids, tannins. Phenolic content was 11.70 mgGAE/g and total flavonoid was 0.281
mgQE/g. Non-specific parameters showed specific gravity 0.5621 g/mL, dry shrinkage content
9.60%, ash content 12.48%, water content 15.21%, metal contamination in mercury (0.24 mg/kg),
lead (0.26 mg/kg) and cadmium (0.008 mg/kg). Microbial contamination in the total plate number
(1x103 colonies/g) and yeast (1x103 colonies/g). The results of the organoleptic evaluation of
biscuit formulations were greenish-yellow, smelled of milk, taste of biscuits, and crunchy texture.
Hedonic test on 30 panelists showed that formula 2 was preferred organoleptically by 63.3% and
biscuit crispness by 50%. The weight uniformity test showed uniform preparation in terms of the
shape and weight of biscuits. Based on these data, it can be concluded that tawaloho leaves can be
used as an active ingredient in biscuits.

Keywords: tawaloho leaf, biscuit, standardization, Southeast Sulawesi

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 31

OP 07

Powerful Natural Antioxidant From Rambutan Rapiah

(Nephelium lappaceum) Rind
Fahrauk Faramayuda*, Julia Ratnawati, Tita Hartyna Sutarna, Oktovia El Shara, Rizka Pratiwi
Faculty of Pharmcy Universitas Jenderal Achmad Yani
*Corresponding author. Email: [email protected]

Rambutan rapiah (Nephelium lappaceum L.) is a plant that is widely distributed in Indonesia. The
rind of rambutan rapiah has been used traditionally as a medicine for fever and dysentery.
Consumption of rambutan rapiah can leave quite a lot of rind waste. So it is necessary to make
efforts to increase the benefits of rambutan rapiah rind, one of which is by exploring its antioxidant
potential. In vitro, an antioxidant activity test was carried out by free radical reduction method 1,1-
diphenyl-2-picrylhydrazyl (DPPH) solution using UV-Visible spectrophotometry, and a cellular
antioxidant activity test was carried out by observing the state of oxidative stress created by
inducing hydrogen peroxide in blood cells. Red. The parameters measured were malonaldehyde
(MDA) and superoxide dismutase (SOD) activity. The methanol extract of rambutan rapiah rind
tested by the DPPH method had an IC50 value of 0.4 g/mL, so it was classified as a strong
antioxidant. MDA levels and SOD activity, methanol extract of rambutan rapiah rind has the good
cellular antioxidant ability, with the best activity at a concentration of 40µg/mL with a lower MDA
level of 0.9129±0.0594 nmol/μL and large SOD activity 14.7123±0.0002 units/mL with an
inhibitory activity of 91.9521%. This very strong antioxidant potential of rambutan rapiah rind can
be the basis for further research that leads to activities to prevent or treat diseases caused by free
radicals such as anti-cancer and anti-inflammatory.

Keywords: Nephelium lappaceum L, rapiah, antioxidant, cellular, non-cellular

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 32

OP 08

Anti-inflammatory effects of Ethanol Extract of Cymbopogon

nardus Herb on Rats Induced By Carrageenan
Diana Puspita Sari1, Ririn Lispita Wulandari2*
1
Pharmacy study program, Faculty of Pharmacy, Universitas Wahid Hasyim, Semarang, Indonesia
2
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Wahid Hasyim,
Semarang, Indonesia

*Corresponding author: [email protected]

Cymbopogon nardus contains many active compounds that are useful in inhibiting the
inflammatory process. This study aims to determine the anti-inflammatory effect of ethanol extract
of Cymbopogon nardus herb on male rats induced by carrageenan. Ethanol extract of Cymbopogon
nardus herb was obtained by maceration with 70% ethanol as solvent. Twenty five male wistar
rats were used in this study. Animals were divided into 5 group: namely the control group positive
(diclofenac sodium 50 mg/kg BW), negative control (Na-Cmc 1 %), and three doses of tested
extract, namely 200 mg/kg BW, 400 mg/kg BW and 800 mg/kg BW. The method used in this
study was paw edema, namely sixty minutes after treatment, all rats were injected by 0.1 mL of
1% carrageenan subplantarly on the rat's right paw. Paw volumes were measured by a
plethysmometer every 60 minutes for 360 minutes, obtained data in the form of the volume of
edema, Area Under the Curve (AUC), and percentage of power anti-inflammatory. The results
showed that the AUC of ethanol extract of Cymbopogon nardus herb at doses of 200 mg/kg BW,
400 mg/kg BW, 800 mg/kg BW was significantly smaller than the negative control (p < 0.05), it
can be concluded that the ethanol extract of Cymbopogon nardus herb has an anti-inflammatory
effect on rats induced by carrageenan. The highest percentage of anti-inflammatory power was
obtained at a dose of 400 mg/kg BW. Ethanol extract of Cymbopogon nardus herb contains
flavonoids, alkaloids, tannins, and saponins.

Keywords: anti-inflammatory, carrageenan, Cymbopogon nardus L.

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 33

OP 09

Acute Toxicity Test of Ethanol Extract of sapodilla stem bark

(Manilkara zapota (L.)) by BSLT Methods
Ratna Widyasari 1, Deska Seria Ramadhanti 1, Reski Kurniawan 1, Yusril Izzamaulana 1, Dina
Yuspita Sari 1
1
Academy of Pharmacy Yarsi Pontianak

*Corresponding author. Email: [email protected]

Sapodilla (Manilkara zapota (L.)) is one of the most widely grown plants in West Kalimantan.
Sapodilla stem bark contains secondary metabolites of flavonoids and saponins, which have
toxicity activity so that it is expected to be an alternative for cancer treatment. The purpose of this
study was to determine whether the ethanol extract of sapodilla stem bark (Manilkara zapota (L.))
is toxic and what is the LC50 value produced by the BSLT. This study used the ethanol extract of
sapodilla stem bark with various concentrations of 0 µg / ml; 100 µg / ml; 250 µg / ml; 500 µg /
ml; 750 µg / ml; 1000 µg / ml with 5 repetitions and each concentration consisted of 10 larvae of
Artemia salina Leach. Data on larvae mortality were collected and analyzed using the probit
analysis method. Based on the regression results, the LC50 value calculated manually or using
Microsoft Excel is 190,54 µg / ml. This study indicates that ethanol extract of sapodilla stem bark
is cytotoxic against the larvae of Artemia salina Leach

Keywords: Sapodilla bark (Manilkara zapota (L.)), Acute toxicity, BSLT (Brine Shrimp Lethality
Test)

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 34

OP 10

SPF Value and Physical Stability of Nutmeg Seed Oil

Microemulsion with Tween 80 and Propylene Glycol as
Surfactan and Cosurfactant
Ayu Shabrina* , Vironica Rizky Fauzia, Aza Zakiyatun Nida, Rizky Adityo, Muhammad
Imaamudin
Faculty of Pharmacy, University of Wahid Hasyim, Semarang

*Corresponding author. [email protected]

The nutmeg seed oil (NSO) can be used as sunscreen, but it is not stable in storage. Microemulsion
can increase the stability of nutmeg oil. The purpose of this study was to determine the physical
stability and sunscreen microemulsion of nutmeg oil (NSM) with variations of tween 80-propylene
glycol (PG) as surfactant and cosurfactant. NSM were made with 6.4% NSO and tween 80-PG
(2:1) variations of 57% (F1), 60% (F2), and 63% (F3). The stability test was carried out for 28
days at a temperature of 25±2°C and RH of 65±5%. The test parameters were SPF value and the
physical stability including organoleptic, phase separation, percent transmittance, particle size, pH,
and viscosity. NSM was also analyzed for its chemical components using GCMS. Quantitative
data (n=3) were statistically analyzed by Paired Sample T-test with a 95% confidence level.
Qualitative data such as GCMS and organoleptic were analyzed descriptively The results showed
that NSM were yellow, clear, with distinctive aroma of nutmeg seed oil, and did not show phase
separation after 28 days of storage. The GCMS result showed the presence of α-pinene, β-
felandren, sabinene, and α-terpinolene compounds. The results of transmittance percentage from
all formulas were in the range of 97.95-99.08%. The particle size results of F1, F2, F3 were 16.5±
6.9; 15.6±7.8; 15.1±5.4. The pH of all formulas is in the range of 6.79-6.81. The viscosity of F1,
F2, and F3 were 1045.82 ± 20.20; 849.82 ± 44.27 and 752.01 ± 14.32 respectively. The results of
the SPF values were 9.245 ± 0.565 (F1), 8.665 ± 0.342 (F2), 8.875 ± 0.756 (F3). All microemulsion
formula using Tween 80 and PG did not change (p > 0.05) during 28 days of storage.

Keywords: Nutmeg seed oil, microemulsion, physical stability, SPF value

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 35

OP 11

Comparison of Cisplatin Drug Toxicity Test and Herbal Drug

Combination of Bovine Serum Albumin (BSA) and Folic Acid
Nanoparticles as Anticancer Candidates
Ersalina Nidianti1, Rizka Amalia1, Nisrina Salsabila Firdaus1
1
Departement of Medical Laboratory Technology, Universitas Nahdlatul Ulama Surabaya, Surabaya, Indonesia

*Corresponding author : [email protected]

The prevalence of cancer in Indonesia has increased by 8.8 percent in the last two years (2018 and
2020) in terms of adding new cases and mortality due to cancer. So that cancer treatment becomes
important in reducing the high number of additional new cases and mortality due to cancer patients.
Intensive cancer treatment is by surgery, chemotherapy, radiotherapy and immunotherapy.
Chemotherapy drugs used for cancer treatment include herbal plant-based cancer drugs and
cisplatin drugs. This study aims to compare the toxicity of herbal drugs with cisplatin drugs, a
combination of BSA nanoparticles and folic acid as anticancer candidates. The research method is
through the synthesis of nanoparticles with the desolvation method, then characterization is carried
out using XRD and FT-IR tests. After that proceed to the toxicity test with the BSLT method. The
results obtained from XRD analysis on BSA and folic acid nanoparticles from cisplatin (As-NP-
BSA-CP) showed a peak of 34.45 while in BSA and folic acid nanoparticles from herbal medicine
(As-NP-BSA-OH) obtained a peak of 22.77. FT-IR analysis on (As-NP-BSA-CP) showed that
there are functional groups O-H alcohol, C-H, C-C, NO2 that play a role in the synthesis of
nanoparticles. Meanwhile, (As-NP-BSA-OH) showed that the functional groups were C-H alkene,
C-O alcohol, and C-N amine/amide. The toxicity level of As-NP-BSA-CP LC50 is 69.14 ppm
while the toxicity level of As-NP-BSA-OH LC50 is 44.14 ppm. Nanoparticles consisting of a
combination of bovine serum albumin (BSA), folic acid and cisplatin drugs or herbal drugs can be
used as candidates for anticancer drugs.

Keywords: anticancer, toxicity, herbal drug, cisplatin drug, nanoparticles

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 36

OP 12

Antioxidant Activity Test of Watermelon (Citrullus lanatus

(Thunb.) Matsum. & Nakai)) Flesh Methanolic Extract with
ABTS and FRAP Method
Nur Herlina Nasir1, Jastria Pusmarani1, Filmaharani1
1
Pharmacy Study Program, University of Mandala Waluya Kendari

*Corresponding author: [email protected]

Watermelon is a local plant that has potential as a source of natural antioxidants. In the white layer
of underutilized watermelon contains substances that are important for health and needed by the
body, one of them is citrulline. Citrulline is one of the antioxidants that is beneficial for skin health.
The aim of this study is to determine the antioxidant activity of the methanolic extract of
watermelon flesh based on its ability to reduce ABTS˙+ and Fe3+ to Fe2+ using ABTS and FRAP.
Extraction of compounds using maceration with methanol p.a. ABTS is based on the formation of
blue color of ABTS˙+ which can be reduced by antioxidants. The extract were serialized with a
number of concentrations and each was reacted with ABTS solution for 2 hours in the dark, the
absorbance was measured at 734 nm. The yield of ABTS radical scavenging was expressed as
equivalent in μM vitamin C/g. FRAP is based on the ability of antioxidant compounds to reduce
Fe3+ to Fe2+ where supernatant is mixed with water and iron(III) chloride, the absorbance is
measured at wavelength 700 nm using spectrophotometer UV-Vis. The reducing activity of the
extract was determined as the equivalent of ascorbic acid (mg of ascorbic acid/g extract). The
result of maceration is 334,491 g with percent yield 16,380. The average percent radical
scavenging (IC50) activity using ABTS is 24.996±0.059 μg/mL with strong category, while the
antioxidant reducing power through FRAP method is 13.677±0.246 mg/g extract.

Keywords : Watermelon, Maceration, ABTS, FRAP, Spectrophotometer UV-Vis

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 37

OP 13

Antibacterial Activity Ethanol Extract of Chinese Petai (

Leucaena leucocephal (Lam.De Wit),guava leaves ( Psidium
guajava L.) and their combinations on the growth of
Escherichia coli Bacteria
Suhaera1*,Yola Selvia1, Yufri aldi2
1
Pharmacy Study Program,Institut Keseahatan Mitra Bunda,Batam,Indonesia
2
Phaculty Pharmacy, Universitas Andalas, Indonesia
*Corresponding author. Email: [email protected]

Chinese petai leaf (Leucaena Leucocephal (Lam.) de wit , Guava leaf (Psidium guajava L.) is one
of the natural ingredients that can be used as an antibacterial. This study aims to determine the
class of secondary metabolite compounds and antibacterial activity against Escherichia coli
bacteria by pouring and paper disc diffusion methods. Simplified Chinese petai leaf (Leucaena
Leucocephal (Lam.) de guava leaves (Psidium guajava L.), and their combination macerated with
70% ethanol. Phytochemical Screening Results of ethanol extract Chinese petai leaf (Leucaena
Leucocephal (Lam.) de wit contain tannins, alkaloids, saponins, and flavonoids, Guava leaf
(Psidium Guajava L.) contain alkaloids, tannins, saponins, and flavonoids. Furthermore, TLC was
carried out to confirm the presence of chemical compounds in the extracts of Chinese petai leaf
(Leucaena Leucocephal (Lam.) de wit, guava leaf (Psidium Guajava L.) and their combination
using the mobile phase N-Hexane: Ethyl Acetate (4:1:5). ) with silica gel GF254 a stationary phase
with 5% FeCl3 spotting for phenolics and 5% AlCl3 for flavonoids then detected at 254 nm and
366 nm UV light. The positive control used Co-trimoxazole 50 g while the negative control was
physiological NaCl. Based on the results, the average diameter of the inhibition zone of the
ethanolic extract of Chinese petai leaf (Leucaena Leucocephal (Lam.) de wit against Escherichia
coli bacteria with a concentration of 25 mg/mL, 20 mg/mL 15 mg/mL was 11.04 mm; 9.32 mm;
7.82 mm, guava leaf ethanol extract (Psidium guajava L.) against Escherichia coli bacteria with a
concentration of 25 mg/mL, 20 mg/mL 15 mg/mL, namely 13.94 mm; 9.88mm; 8.7 mm and
ethanol extract combination of Chinese petai leaf (Leucaena Leucocephal (Lam.) de wit and guava
leaf (Psidium Guajava L.) with a concentration of 25 mg/mL, 20 mg/mL 15 mg/mL, namely 14.98
mm ; 12.96mm; 11.46mm. Based on the results of the One Way Anova test, it showed the effect
of antibacterial activity on Escherichia coli with a significant value of 0.00<0.05. This indicates
that the ethanol extract has activity on Gram-positive.

Keywords : Antibacterial, ethanol extract, chinese petai leaf (Leucaena Leucocephal (Lam.) de
wit , guava leaf (Psidium Guajava L.), Escherichia coli.

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 38

OP 14

Antibacterial activity of Meistera chinensis rhizome extract

on Staphylococcus aureus and Escherichia coli bacteria
by agar diffusion method
Karmilah, Reymon, Nur Saadah Daud, Yulianti Fauziah, Musdalipah*
Departement of Pharmacy, Politeknik Bina Husada, Kendari, Indonesia
*Corresponding author. Email: [email protected]

Meistera chinensis is one of the new generations of the Zingiberaceae family that grows a lot in
Southeast Sulawesi. Empirically used as a spice and increase immunity. Previous data on fruit
extract contains secondary metabolites such as flavonoids, terpenoids, alkaloids, steroids, and
tannins. Fruit extracts are efficacious as antioxidants, antibacterial, and toxicity. There has been
no report on the aspect of the antibacterial effect on the rhizome. This study aims to determine the
antibacterial activity of Meistera chinensis rhizome ethanol extract. Meistera chinensis rhizome
dry powder was extracted by maceration method using 96% ethanol as solvent. The extract was
concentrated using a rotary vacuum evaporator. Antibacterial Activity test method using agar
diffusion. The concentration of ethanol extract of Meistera chinensis rhizome was made into 5
treatment groups with concentrations of 10%, 20%, 30%, and 2 control groups, positive control
(Ciprofloxacin 30µg) and negative (Aquadest). The antibacterial activity test was carried out using
the agar diffusion method against Staphylococcus aureus ACTT 25023 and Escherichia coli
ACTT 35218 for 24 hours and incubated at 37oC, then the inhibition was measured. Data were
analyzed using One Way ANOVA statistical method. The results showed that the ethanol extract
of Meistera chinensis rhizome could inhibit the growth of Escherichia coli with an average
diameter of the inhibition zone at concentrations of 10% (6.08 mm), 20% (8.16 mm), and 30%
(10.57 mm) respectively. In Staphylococcus aureus, the diameter of the inhibition zone is 5.02
mm, 10 mm and 8.033 mm, respectively. The test results on the positive control Ciprofloxacin 30
g was 10.28 mm, and the negative control Aquadest was 0 mm. The results of the antibacterial
activity analysis showed that there was a significant difference (p = 0.00 < 0.05) in bacterial
growth. Based on these data, it can be concluded that the rhizome of Meistera chinensis has
antibacterial properties.

Keywords: antibacterial, Staphylococcus aureus, E.Coli, Meistera chinensis rhizome

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 39

OP 15

Antioxidant Activity of Face Mist From Red Dragon Fruit Peel

Extract (Hylocereus polyrhizus) With DPPH Method
Sri Wahyuningsih*, A. Juaella Yustisi, Muhammad Asri SR1, Nurmala1
Faculty of Pharmacy, Department of Pharmacy, Megarezky University

*Corresponding author. Email: [email protected]

Air pollution can lead to the formation of free radicals and antioxidants can prevent the oxidation
of free radicals. Red dragon fruit peel contains vitamin C, vitamin A, alkaloids, flavonoids,
polyphenolic tannins which have antioxidant activity. Face mist is one of the facial cosmetic
products that contain antioxidants that can prevent free radicals. This study aims to formulate a
face mist from red dragon fruit peel extract (Hylocereus polyrhizus) and to determine the
antioxidant activity of face mist. The face mist was formulated by the composition of red dragon
fruit peel extract with various concentrations F1 (1%), F2 (3%), and F3 (5%) and determined
antioxidant activity with the DPPH method. The result showed that the face mist had good
physicochemical stability because there was no difference before and after the cycling test.
Antioxidant activity in F1 (1%) was 39.84 ppm, F2 (3%) was 23.57 ppm and F3 (5%) was 19.26
ppm. it can be concluded that the greatest antioxidant activity is found at a concentration of F3
(5%) with an IC50 of 19.26 with a very strong category.

Keywords: Face mist, red dragon fruit peel, antioxidant activity, DPPH

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 40

OP 16

Analgesic Activity of Dadap Serep (Erythrina subumbrans

(Hassk.) Merr.) Leaf
Saru Noliqo Rangkuti, Arini Aprilliani
Sekolah Tinggi Farmasi Muhammadiyah Tangerang

*Corresponding Author : [email protected]

Dadap serep leaf (Erythrina subumbrans (Hassk.) Merr.) is a plant that is used hereditary by the
community. The content of compounds contained in the leaves of dadap serep one of them is
flavonoids which have biological activity as analgesics. This study aims to determine analgesic
activity, optimal dosage, and its comparison with positive control of 70% ethanol extract from
leaves to spare. This research is an experimental study with a complete randomized study design.
The method used for the analgesic test is the stretching method. Test animals used were male white
mice. Mice were divided into 5 groups, namely group I as a negative control using 0.5% Na-CMC,
group II as a positive control using ibuprofen, groups III, IV and V as a treatment group of 70%
ethanol extract of leaves with spare doses at a dose of 5.6 mg / 20 gBB, 11.2 mg / 20 gBB, and
22.4 mg / 20 gBB. The data obtained were then analyzed statistically with one way ANOVA. The
results obtained showed that the dose of 70% ethanol extract of leaves of spare dadap can provide
activity as an analgesic to mice and the optimal dose as analgesics is at a dose of 22.4 mg / 20 gBB
of mice. Based on data from analgesic activity test results were analyzed by one way ANOVA
method and continued with post hoc test results obtained p ≤ 0.05.

Keywords: Dadap Serep Leaf (Erythrina subumbrans (Hassk.) Merr.) and Analgesic

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 41

OP 17

Formulation and Qualitatif Analysis of Sea Buckhthorn Oil

Microemulsion using Isopropyl Myristate
Rapfika Setiyaningrum, Ayu Shabrina*
Faculty of Pharmacy, University of Wahid Hasyim, Semarang

*Corresponding author. [email protected]

Sea buckhthorn oil (SBO) consists of palmitic acid, oleic acid and linoleic acid which can be used
as antiaging. Microemulsion using isopropyl miristate (IPM) can increase the efficacy and
penetration of SBO. The purpose of this study was to determine the physical properties and the
content of SBO in Microemulsion using IPM. Microemulsion were made with 7% SBO and IPM
variations of 1% (F1), 2% (F2), and 3% (F3). The test parameters were physical properties
including organoleptic, phase separation, percent transmittance, particle size, pH, and viscosity.
SBO microemulsion was also analyzed for its chemical components using GCMS. Quantitative
data (n=3) were statistically analyzed by one way Anova with 95% confidence level. Qualitative
data such as GCMS and organoleptic were analyzed descriptively The results showed that SBO
microemulsion were orange, clear, with distinctive aroma of SBO. The GCMS result showed the
presence of palmitic acid, pentadecanoic acid, linoleic acid and palmitoleic acid compounds. The
results of transmittance percentage from all formulas were in the range of 97.95-99.08%. The
particle size results of F1, F2, F3 were 19.0± 16.9; 17.0±12.0; 18.4±15.7 (P>0,05). The pH of all
formulas is in the range of 7.53-7.58. The viscosity of F1, F2, and F3 were 113.19 ± 5.66; 109.86
± 1.77 and 90.84 ± 11.48 (P>0,05) respectively. All microemulsion formula using IPM had good
physical properties.

Keywords: Sea buckhthorn, microemulsion, physical properties, GCMS

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 42

OP 18

Formulation and In Vitro SPF Value Test of Sea Buckhthorn

Oil Microemulsion using Tween 80-PEG 400 as Surfactant
and Cosurfactant
Savna Dyah, Ayu Shabrina*
Faculty of Pharmacy, University of Wahid Hasyim, Semarang

*Corresponding author. [email protected]

Sea buckhthorn oil (SBO) consists of β-carotene which can be used as sunscreen. Microemulsion
using tween 80 and PEG 400 can increase the stability and efficacy of SBO. The study aimed to
determine the physical properties and in vitro SPF value of SBO Microemulsion using Tween 80
and PEG 400. Microemulsion were made with 7% SBO and Tween 80-PEG 400 variations of (7:6)
(F1), (8: 6) (F2), and (9:6) % (F3). The test parameters were physical properties including
organoleptic, phase separation, percent transmittance, particle size, pH, and viscosity and also in
vitro SPF value. Quantitative data (n=3) were statistically analyzed by one way Anova with 95%
confidence level. Qualitative data such as organoleptic properties was analyzed descriptively
Theresults showed that SBO Microemulsion were Orange, clear, with distinctive aroma of Sea
Buckthorn oil. The results of transmittance percentage from all formulas were in the range of
97.95-99.08%. The particle size results of F1, F2, F3 were 15.8±9.4; 15.7±10.2; 16.8±9.9. The pH
of all formulas is in the range of 7,53- 7,55. The viscosity of each formula showed a difference (p
< 0.05) with the following data were (125.25 ± 6.01) F1; (132.96 ± 2.99) F2 and (116.17 ± 3.84)
F3. respectively. The results of the SPF values were 8.6 ± 0.4 (F1), 9.4 ± 1.0 (F2), 9.4 ± 0.5 (F3).
The SPF value of SBO microemulsion formula using Tween 80 and PEG 400 were categorized as
maximum protection.

Keywords: Sea buckhthorn, microemulsion, physical properties, GCMS

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 43

OP 19

Formulation and Antibacterial Activity of Hand Sanitizer Gel

Containing Watermelon Peel (Citrullus lanatus) Extract
Deo Julian Hikmat1*, Filmaharani1, Yaya1, Nur Hatidjah Awaliyah1, Jastria Pusmarani2
1
Department of Pharmacy, Universitas Mandala Waluya, Kendari, Indonesia
2
Department of Clinical Pharmacy and Pharmacology, Universitas Mandala Waluya, Kendari, Indonesia

*Corresponding author: [email protected]

Hand sanitizer is a preparation used to maintain health and hygiene of hand, and its is used more
practical. Hand sanitizers are available in liquid or gel form. Generally, hand sanitizer gels are
alcohol-based. However, continuous use of alcohol cause irritate the skin.Therefore, we are
necessary for alternative natural ingredients that can be used to prevent skin irritation. One of the
natural ingredients that proven to be antibacterial is watermelon peel (Citrullus lanatus). The
purpose of this study was to look the potential of hand sanitizer gel of watermelon peel extract
against Escherichia coli (E. coli) and Staphylococcus aureus (S. Aureus). The method of research
is used empirical research with offline platform by implementing strict health protocols during the
covid-19 pandemic. The methanol extract of watermelon peel was tested for antibacterial against
E. coli and S. aureus bacteria with three concentrations 15%, 17.5% and 20%, respectively. The
formulation of hand sanitaizer was made with different concentration of carbopol as many as 1%,
1.5% and 2%. Furthermore, it is evaluated the physical properties of the gel preparation. Methanol
extract of watermelon peel with concentration 15% have antibacterial activity on against E.coli
and S. aureus bacteria with an average inhibition zone value as many as 5.03 mm and 2.5 mm,
respectively. Formula 1 (carbopol 1%) and formula 2 (carbopol 1.5%) are met the requirements for
the physical properties of the gel preparation include organoleptic, pH, viscosity, homogenity and
dispersibility tests. Meanwhile, formula 3 (2% carbopol) is does not meet the physical test include
the dispersion test. Conclusion of the research that, formulation of hand sanitizer gel containing
watermelon peel (Citrullus lanatus) demonstrated antibacterial activity and it meets requirements
for the physical properties of the gel preparation.

Keywords: antibacterial activity, hand sanitizer, gel, watermelon peel

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 44

OP 20

Determination of Betamethasone and Dexchlorpheniramine

Maleate in Tablet Dosage Form by TLC Video Densitometry
Method
Winasih Rachmawati*, Anggina Widya, Ivan Andriansyah
Faculty of Pharmacy, Bhakti Kencana University, Bandung, 40614, West Java, Indonesia
*Corresponding author:: [email protected]

An analytical method for the determination of betamethasone and dexchlorpheniramine maleate

mixtures in tablets has been developed by the TLC video densitometry method. The
chromatographic separation of the two analytes was done in a TLC GF254, using a mixture of
ethanol and ammonia (14:1, v/v) as a mobile phase. Visualization and spotting recording analysis
utilizing a set of densitometric video tools and measuring the area under the curve (AUC) were
analyzed by ImageJ software. The Rf value of betamethasone and dexchlorpheniramine maleate
were 0.8 and 0.28, respectively. The recovery for betamethasone and dexchlorpheniramine maleate
was found to be 95.1± 0.5% and 103.79 ± 0.3%, respectively. The limit of detection was 3.786
and 57.78 μg/ml with the relative standard deviation (RSD) of 1.21 and 1.28 % for betamethasone
and dexchlorpheniramine maleate respectively. The results showed that the betamethasone and
dexchlorpheniramine maleate levels for samples 1 were 98,29% and 101,78% and for sample 2
were 98,34% and 101,55%. The validation parameter values suffice the established requirements.
This method can be applied to determine betamethasone and dexchlorpheniramine maleate levels
in a dosage form.

Keywords: betamethasone, dexchlorpheniramine maleate, TLC video densitometry, validation

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 45

OP 21

Antioxidant Activity Assay Of Water Fraction, Ethyl Acetate

Fraction And N-Hexan Fraction Of Banana Peel (Musa
paradisiaca Var. Sapientum) Extract
Jastria Pusmarani1*, Ulfa2, Citra Dewi2
1
Department of Pharmacology and Clinical Pharmacy of Universitas Mandala Waluya, Kendari, Indonesia
2
Department of Pharmacy of Universitas Mandala Waluya, Kendari, Indonesia

*Corresponding author: [email protected]

Free radical and reactive oxygen species as inducers of cellular and tissue pathogenesis which
causing some diseases such as diabetes, cancer and cardiovascular. Therefore, they need a
compound that eliminate free radical namely antioxidant. Banana peel (Musa paradiasiaca var.
Sapientum) is containing flavonoid and phenolic compounds that have potential antioxidant. This
study aim to determine antioxidant activity of water fraction, ethyl acetate fraction and n-hexan
fraction of banana peel (Musa paradiasiaca var. Sapientum) extract by 2,2-diphenyl-1-picryl-
hydrazylhydrate (DPPH) method. Banana peel was macerated with methanol. Furthermore,
methanolic extract of banana peel was made into water, ethyl acetate, and n-hexan fraction using
liquid-liquid partition method. All fractions of banana peel extract was carried out at 100, 200,
300, 400, and 500 ppm and vitamin C as positive control. The absorbance was measured with UV-
Visible spectrophotometer at 517 nm. The results of this study that ethyl acetate fraction has
moderate antioxidant activity with inhibitory concentration 50% (IC 50) value of 139.498 ppm. The
n-hexan fraction and water fraction of banana peel have an antioxidant activity with IC 50 value of
875.529 ppm and 358.395 ppm, respectively. This research showed that all fractions of banana
peel have antioxidant activity with the highest IC 50 from ethyl acetate fraction.

Keywords: Antioxodant, fraction, banana peel, DPPH.

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 46

OP 22

In Silico Study of Xanthones, Alpha-Mangostin, And Gamma-

Mangostin As Anti-Diabetic Drug Candidate
Rifa’atul Mahmudah1,2*, I Ketut Adnyana1, Elin Yulinah Sukandar1
12
Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, Bandung Institute of Technology,
West Java, Indonesia
2
Department of Pharmacy, Faculty of Science and Technology, Mandala Waluya University, Kendari, Southeast
Sulawesi, Indonesia

Corresponding author: [email protected]

The current use of herbs as complementary and/or alternative medicines is becoming increasingly
popular throughout the world. Herbs are often given in combination with therapeutic drugs that
have the potential to cause drug-herb interactions. Several studies have shown the benefits of herbs
as a complement to therapeutic drugs, including in the treatment of diabetes mellitus (DM).
Diabetes mellitus is a chronic metabolic disease characterized by elevated blood glucose levels,
which over time causes serious damage to the heart, blood vessels, eyes, kidneys and nerves. The
International Diabetes Federation (IDF) estimates that at least 463 million people aged 20-79 years
in the world will suffer from this disease in 2019 and continue to increase in 2045. Meanwhile,
Indonesia ranks 7th among the countries with the highest incidence rates worldwide, with the
percentage of people with diabetes mellitus reaching 10.7 million in 2019. The high incidence of
diabetes mellitus requires effective treatment options. Mangosteen rind (Garcinia mangostana L.)
is one of the herbs that has an antihyperglycemic effect. This material is easy to find in Indonesia
and has been widely known and used by the public. Compounds of xanthones, alpha-mangostin,
and gamma-mangostin contained in the mangosteen rind were used as markers. However, the use
of mangosteen rind extract in the treatment of DM requires information on standardization of raw
materials, efficacy, safety, and interactions that occur when used together with oral
antihyperglycemic drugs. This research studies was conducted using natural ligands consisting of
xanthones, alpha-mangostin, and gamma-mangostin. The training set was designed using
Autodock 4.2 software for several receptors that play a role in treating diabetes mellitus. The
results showed that the binding affinity of each compound (xanthones, alpha-mangostin, gamma-
mangostin) was almost the same as the comparison ligand at the SGLT-2 receptor. However, -
mangostin compound had the most negative binding free energy value (-11.61) when compared to
-mangostin and xanthone compounds (-2.50; -1.66). This shows that the compound has the best
bonding ability which is expected because of the several hydrogen bonds it has. Meanwhile, in
observing its activity on GLP-1, GSK-3 and , and CD-4, each compound (xanthones, alpha-
mangostin, and gamma-mangostin) showed the ability to bind to amino acid receptors as indicated
by the value of its binding affinity. From the results of this study, it can be concluded that
xanthones, -mangostin, and -mangostin compounds can be developed as candidates in the
treatment of diabetes mellitus.

Keywords : Diabetes melitus, Garcinia mangostana L., xanthones, alpha-mangostin, gamma-

mangostin.

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 47

OP 23

Acute Toxicity Test With BSLT Method And Antioxidant

Activity With FRAP Method From Ethanol Extract Of Teak
Leaves (Tectona grandis L.)
Nuralifah1*, Parawansah 1,2, Waode Marianti1
1
Department of Pharmacology, Faculty of Pharmacy, Halu Oleo University, Kendari
2
Department of Pharmacology, Faculty of Medicine, Halu Oleo University, Kendari

*Email correspondent author: [email protected]

Toxicity is the ability of a substance to cause damage that has an impact on organisms so that
toxicity tests can be used to determine the dosage for human safety. Natural antioxidants can
protect the body against damage caused by reactive oxygen compounds, inhibit the occurrence of
disease, and can inhibit lipid peroxidation in food. Teak leaves (Tectona grandis L.) are thought
to have antioxidant potential because they contain flavonoid compounds. This study was
conducted to know the toxicity of the ethanol extract of teak (Tectona grandis L.) leaves by using
the BSLT (Brine Shrimp Lethality Test) method and to determine the antioxidant activity of the
ethanolic extract of teak (Tectona grandis L.) leaves using the FRAP method ( Ferric Reducing
Antioxidant Power). The extract was obtained by maceration method using 96% ethanol solvent
for 3×24 hours and concentrated at 40 0C. The results of the BSLT test on the ethanol extract, the
LC50 value was 1149.40 g/ml. Meanwhile, to test the antioxidant activity of the ethanol extract of
teak (Tectona grandis L.) leaves, the IC50 value was 62.236 g/ml. This study concludes that the
ethanol extract of teak (Tectona grandis L.) leaves has no toxic effect seen from the LC 50 value of
1149.40 g/mL in the low category and the antioxidant activity test of the ethanol extract of teak
(Tectona grandis L.) leaves has the antioxidant activity of 62.236. g/ml with a strong antioxidant
category.

Keywords : Toxicity, antioxidant, IC50, LC50

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 48

OP 24

Antioxidant and Antihiperurisemia Activity Assay of Aegle

marmelos L Leaves
Risky Juliansyah Putri*, Bai Athur Ridwan, Uyun Wardarini, Syamsiah Pawannei
Department of Pharmacy, Universitas mandala waluya

*Email correspondent author : [email protected]

The use of plants to prevent various diseases is still widely used by Indonesian community like the
Aegle marmelos leaves. It is contains metabolite compounds include flavonoids, alkaloids, tannins
and saponins. They are potentially as antioxidants and reducing the activity of the enzyme xantin
oxidase by inhibited oxidative stress and reduced uric acid production. This study aim to find out
the antioxidant and antihyperurisemia activity of Aegles marmelos L. leaves ethanol extract. The
Aegles marmelos L. leaves was using method extraction such as maceration. Furthermore, the
extract is carried out a phytochemical screening test of its metabolite compounds. Antioxidant
assay were conducted using the ferric reducing antioxidant power (FRAP) method and
measurement of antihyperuricemia activity using photometric 2,4,5-tribhromo-3-hidroxy-
benzoate-acid (THBA) at 546 nm Uv-Vis spectrophotometry. The results of this study showed that
the ethanol extract of Aegles marmelos L. leaves containing secondary metabolite compounds such
as alkaloids, flavonoids, tannins, saponins and phenols. The antioxidant activity value of ethanol
extract of Aegles marmelos L. leaves is 9,518 mgQE/g extract. Antihyperurisemia activity had
significant differences of p=0.000<0.05. The percentage of decrease in uric acid levels by ethanol
extract of Aegles marmelos L. leaves is the greatest at doses of 350 mg / KgBW of
44.08% comparing to doses of 150 mg / KgBW and 250 mg / KgBW as many as 22.56% and
31.18%, respectively. It can be concluded that ethanol extract of Aegles marmelos L. leaves has
antioxidant activity and can reduce uric acid levels in mice (Mus musculus).

Keywords : Antioxidant, Antihyperuricemia, Aegle marmelos L.

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 49

OP 25

Phytochemical Constituent And In Vitro Cytotoxic Activity Of

Hibiscus Sabdariffa L. Calyx Fraction On Human Breast
Cancer Cell Line MDA-MB-231
W.O. Yuliastri1,2*, A. Diantini2, M. Ghozali3, I. Sahidin4, E.Julaeha, M. Isrul2
1
Faculty of Pharmacy, Universitas Padjajaran, Bandung-45353, West Java, Indonesia
2
Departement of Pharmacy, STIKES Mandala Waluya Kendari, Kendari-93213, South-East Sulawesi, Indonesia
3
Departement of Biochemistry and Molecular Biology, Universitas Padjajaran, Bandung-45353, West Java,
Indonesia
4
Faculty of Pharmacy, Universitas Halu Oleo, , Kendari-93213, South-East Sulawesi, Indonesia

*Correspondent author: [email protected]

Hibiscus sabdariffa L. is a medicinal plant that has various pharmacological activities. One of the
activities of Hibiscus sabdariffa L. is the immunomodulatory activity closely connected with the
anticancer activities. This study aimed to test the cytotoxic activity of Hibiscus sabdariffa L. Calyx
Fractions against breast cancer cells (MDA-MB-231), determine the active fractions, and analyze
the compounds of the active fraction of Hibiscus sabdariffa L. with LC-MS. The Hibiscus
sabdariffa L. Calyx was fractionated using vacuum liquid chromatography. Meanwhile, the
cytotoxic activity was examined using the MTT method. The test results discovered that the
Fraction A of Hibiscus sabdariffa L. Calyx is the active fraction with a score of IC50 187.89 µg/ml.
The identification of Fraction A's chemical compounds using LC-MS discovered three identified
compounds: tert-butyl-4-methoxyphenol, Nigakilactone H, and Stigmastan-3,6-dione. Since these
compounds potentially function as active cytotoxic compounds, it is suggested that further studies
isolate the active compounds from Fraction A of Hibiscus sabdariffa L.

Keywords: Hibiscus sabdariffa L., Cytotoxic, Fraction, Breast cancer, MDA-MB-231

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 50

OP 26

Hair Growth Promoting Activity of Langir Bark (Albizia

Saponaria Lour.) Ethanol Extract: In vivo Assay

Himaniarwati1,2, Muhammad Arba4, Yasmiwar Susilawati3, Resmi Mustarichie1

1
Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran,
Indonesia
2
Departement of Pharmacy, Universitas Mandala Waluya, South-East Sulawesi, Indonesia
3
Department of Pharmacy Biology, Faculty of Pharmacy, Universitas Padjadjaran Indonesia
4
Faculty of Pharmacy, Halu Oleo University, Kendari, Indonesia
*Correspondent author:: [email protected]

Hair loss and baldness are problems which is often found in clinical dermatology terms. This study
aims to determine the ethanol extract of Albizia saponaria stem bark as a hair growth stimulant.
The barks of Albazia saponaria has collected from the Tolaki tribe, South Konawe, Southeast
Sulawesi. Extraction was carried out by maceration and it’s fractionated by using liquid-liquid
extraction methods. Phytochemical screening according to the Farnsworth method. The hair
growth activity for adult male rabbits is using the modified Tanaka method. The phytochemical
screening showed that the ethanol extract contained tannins, alkaloids, flavonoids, saponins,
phenols, and triterpenoids. The results of the hair growth activity showed that the ethanol and water
extract of concentrations 5%, 10%, 15%, and 20% were significantly fertilized the rabbit male
hairs for 24 days. The ethanol extract and water fraction with a concentration of 15% albizia barks
have been showed a p value <0.05. The results showed that the ethanol extract and water fraction
of albizia Saponaria barks was effective for rabbit hair growth and Albizia Saponaria has the
potential for hair growth.

Keywords: Hair Growth, Ethanol extract, Albizia sponaria, Phytochemical screening

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 51

OP 27

Immunomodulatory Potency Of Hibiscus sabdariffa L.

Fraction On Interleukin 12 (Il-12) Levels In Balb/C Mice (Mus
musculus)
Wa Ode Yuliastri, Dyah Ayu Kusumo Winahyu
Mandala Waluya University
*Correspondent author : [email protected]

Immunomodulators are substances or drugs that can modulate the function and restore the activity
of the immune system. One of the plants that can increase the potential of immunomodulators is
Hibiscus sabdariffa L. Secondary metabolite compounds contained in Hibiscus sabdariffa L. are
flavonoid compounds. Thus making this plant has natural antioxidant activity that can increase
immunomodulatory potential. This study aimed to determine the immunomodulatory potency of
Hibiscus sabdariffa L. Fraction and the vacuum liquid chromatography (KCV) fraction that
provided activity as an immunomodulator to the levels of Interleukin-12 (IL-12) in Balb/C mice
(Mus musculus). This study was analytical research. The samples were extracted by maceration
method using 96% ethanol solvent and in KCV for glycoside separation, and then TLC was carried
out to obtain fractionation. After obtaining 7 fractions, and then given treatment to 10 groups of
test animals for 7 days by administering fraction suspension orally at a 600 g/mL dose. Group 1
and 2 were given a non-polar fraction. Group 3, 4 and 5 were given a semi-polar fraction. Group
6 and 7 were given a polar fraction, Group 8 was given stimuno®. Group 9 was given Na-CMC
and group 10 was given feed. On the 8th day, the test animals were induced with a 0.5 mL
Staphylococcus aureus bacterial suspension intraperitoneally. Then, the immunomodulator
potency was tested for Interleukin-12 (IL-12) level using the Elisa device with the IL-12 kit using
the Sandwich method. Then, the data were analyzed using the One Way ANOVA statistical test
method and the Post Hoc Tamhane test. Based on the results of the study, the KCV fraction of
Hibiscus sabdariffa L. That had the best immunomodulatory potential was in group 4, fraction 4
(semi-polar). This shows that the average value of the semi-polar fraction 4 was related to
flavonoid content with an activity value of (96,051 ng/mL), because it was close to the value of
the positive group (89,525 ng/mL).

Keywords : Immunomodulator, Hibiscus sabdariffa. L, Interleukin-12 levels, Mus musculus

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 52

OP 28

Formulation and antioxidant activity of lip balm ethanol

extract paper flowers (Bougainvillea spectabilis)
Nur Hatidjah Awaliyah Halif, Risky Juliansyah, Reni Pratiwi
Pharmacy Study Program, University of Mandala Waluya
*Correspondent author : [email protected]

Paper flowers (Bougainvillea spectabilis) is a plant that is often used as an ornamental plant. Its
contains alkaloids,saponins, tannins, phenolics, flavonoids, triterpenoids and glycosides
compounds so that it has the ability to capture large free radicals which are needed to prevent
oxidation on the surface of the lip skin. This study aimed to formulate and determine the physical
stability of lip balm ethanol extract paper flowers, as well as to determine the antioxidant activity
of preparation contain extract concentrations of 1%, 2%, and 3% w/v. This research is an
experimental analysis. Paper flowers (Bougainvillea spectabilis) was extracted by maceration
method with ethanol as solvent. The formulation was carried out with various extract
concentrations of 1%, 2%, and 3% in the form of lip balm preparations. Lip balm preparations
were tested for organoleptic, homogeneity and antioxidant tests using the DPPH method. The
results showed that lip balm preparations with varying concentrations of extracts 1%, 2%, and 3%
gave a physically stable preparation as seen from the organoleptic and homogeneity results.
antioxidant test of paper flowers ethanol extract lipbalm preparations showed strong antioxidant
categories with IC50 values of 1 %, 2%, 3% of 3.28 µg/ml, 5.596 µg/ml, and 6.92 µg/ml,
respectively.

Keywords : paper flowers, antioxidant, lip balm

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 53

OP 29

Immunomodulatory Potency Of Hibiscus Sabdariffa L.

Fraction On Cluster Of Differentiation 8 (CD8) Levels In
Balb/C Mice (Mus Musculus)
Nur Apsa Savitri, Wa Ode Yuliastri, Muhammad Ilyas Yusuf
Pharmacy Departement, Mandala waluya University
*Correspondent author : [email protected]

Immunomodulators are substances that can restore and repair the immune system whose function
is impaired or suppress excessive immune function. One of the medicinal plants used as an
immunomodulator is Hibiscus sabdariffa L. Secondary metabolite compounds contained in rosella
flowers are flavonoids that can increase the work of the immune system (immunomodulator). This
study aimed to determine the immunomodulatory potential of the Hibiscus sabdariffa L. KCV
yield on CD8 levels in balb/c mice (Mus musculus) and to determine which fraction of Hibiscus
sabdariffa L. had the best activity as an immunomodulator of CD8 levels in balb/c mice (Mus
musculus). This study used 30 mice divided into 10 groups. Groups 1 and 2 were given a non-
polar fraction, Group 3,4 and 5 were given a semi-polar fraction, Group 6 and 7 were given the
polar fraction of rosella flowers with a concentration of 600 g/mL, while Group 8 was given
stimuno® 0.5 ml/20g BW as a positive control, Group 9 was given Na-CMC 0.5% w/v as a
negative control and Group 10 was only fed as normal control. Each group was given treatment
for 7 days orally. On the 8th day, the test animals were induced with 0.5 mL of Staphylococcus
aureus bacteria suspension via intraperitoneal injection. Then, the immunomodulator potency was
tested for CD8 levels using an ELISA with CD8 Kit and the Sandwich method. The data analysis
method used the One Way ANOVA statistical test and the LSD Post Hoc Test. The results showed
that the rosella flower KCV yield fraction had potential as an immunomodulator, the rosella flower
(Hibiscus sabdariffa L.) KVC yield fraction which had the best immunomodulatory potential was
3 (semi-polar) fraction related to the flavonoid content of flavonoids with an activity value of
13,266ng /mL) because it was close to the activity value of the positive control group (12,249
ng/mL).

Keywords : Immunomodulator, KCV fraction of rosella flower (Hibiscus sabdariffa L.), CD8
levels

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 54

OP 30

Active Anti Alopecia Chemical Identification Of Merremia

peltata Leaves And Computational Study Toward Androgen
Receptor Using Molecular Docking And Molecular Dynamic
Simulation
Syawal Abdurrahman1,3, Ruslin2, Aliya Nur Hasanah1, Resmi Mustarichie1
1
Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran,
45363, Indonesia.
2
Department of Medicinal Chemistry, Faculty of Pharmacy, Universitas Halu Oleo, 93231, Indonesia
3
Departement of Medical Laboratory Technology, Universitas Mandala Waluya, 93231, Indonesia
*Correspondent author : [email protected]

Alopecia is a health condition in which the hair loses its function in some or all of the body.
Alopecia occurs due to various genetic, environmental and nutritional factors. One of the methods
developed to treat alopecia is through the inhibition of the 5-α-reductase enzyme which converts
testosterone into its more potent metabolite, dihydrotestosterone (DHT). In ethnomedicine, the
leaves of Merremia peltata are used by the people of Sulawesi as a remedy for baldness. Therefore,
in this study an in vivo study was conducted on rabbits to see the anti-alopecia activity of the
ethanolic extract of M. peltata leaves. Furthermore, the purified extract of M. peltata leaf was
fractionated using vacuum liquid chromatography method with several solvents and produced
fractions (A-E). The fraction (A-E) was then retested in vivo in rabbits, the fraction was then
analyzed for its content by LC-MS. An in silico study was then carried out using minoxidil ligand
as a comparison and 17 compounds derived from the identification of LCMS/MS against the
fraction (A-E) resulting from vacuum liquid chromatography of M. peltata leaves which were
identified as anti-alopecia compounds through molecular interaction prediction, molecular
dynamics simulation and prediction of absorption, distribution, metabolism, excretion, and
toxicology (ADME-Tox). The assay results showed that the A-E fraction gave the best activity on
hair growth compared to the positive control, and the test compound obtained from the LC-MS
analysis was bufotalinin which gave strong binding energy to the receptor in the molecular docking
interaction study. The bond energy value of Bufotalinin compound was -5.99 kcal/mol while
minoxidil as a comparison yielded -4.8 kcal/mol. Molecular dynamic simulation analysis with
complex stability parameters based on solvent-accessible surface area (SASA), principal
component analysis (PCA), the root mean square deviation (RMSD), and root mean square
fluctuation (RMSF) showed that compound 2 bufotalinin has good affinity to androgen receptors.
ADME-Tox prediction for compound 2 (Bufotalinin) showed good results on the parameters of
skin permeability, absorption and distribution. Therefore, compound 2 (Bufotalinin) class of
steroid compounds is a potential androgen receptor antagonist and is useful in the treatment of
alopecia.

Keywords: alopecia, 5‑α‑reductase, Merremia peltata, molecular docking, molecular dynamics,

ADME‑Tox.

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 55

OP 32

Potential Imunomodulator Fraction Of Rosella Flower

(Hibiscus Sabdariffa L.) On Gamma Interferon Levels (Inf-γ) In
Balb/C Mice (Mus Musculus)
Wa Ode Yuliastri,Niluh Eky Avisvani A.S
Pharmacy Departement, Mandala Waluya University
*Correspondent author : [email protected]

Immunomodulator is a substance that can modulate (change or influence) the body's immune
system to be normal. Rosella flower (Hibiscus sabdariffa L.) is one of the plants that can be used
as an immunomodulator because the content of phenolic compounds and flavonoids in rosella
flower (Hibiscus sabdariffa L.) can increase the body's immune system (immunomodulator). The
purpose of this study was to determine the immunomodulatory potential of the rosella flower
(Hibiscus sabdariffa L.) KCV yield fraction on IFN-γ levels in balb/c mice (Mus musculus) and
also to determine the rosella flower (Hibiscus sabdariffa L.) KCV yield fraction. This can provide
activity as an immunomodulator to IFN-γ levels in balb/c (mus musculus) mice. This researchwas
an analytical study, the samples were extracted by maceration method with 96% ethanol as solvent.
Then the vacuum liquid chromatography (KCV) fractionation process was carried out.
Furthermore, 30 male mice of the Balb/c strain were divided into 10 groups and tested for 7 days
and on the 8th day intraperitoneally, each group was suspended with 0.5 ml of Staphylococcus
aureus. Then dissected and the plasma was taken. Then the IFN - gamma levels were tested using
ELISA and the data were analyzed statistically using the LSD post hock method. The results
showed that the roselle flower (Hibiscus sabdariffa L.) fraction had potential as an
immunomodulator for IFN-γ levels in bal/c mice (Mus musculus) and the fraction that had an
average value close to positive control was fraction 1 (Non polar), fraction 3 (semi polar) and
fraction 5 (semi polar). Which is fraction 5 which is semi-polar has the best activity as an
immunomodulator against IFN-γ levels in mice with an average value of 410.738ng / mL

Keywords : Immunomodulator, Fraction, Hibiscus sabdariffa L., Interferon Gamma (IFN- γ),
ELISA

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 56

OP 33

The Potential Leaves Of Porang (Amorphophallus muerelli

blume) As Antioxidant And Antibacterial againts Escherichia
coli and Salmonella typhi
Susanti Erikania, Vivi Rosalina
Pharmacy Departement, STIKES Bhakti Husada Mulia
*Corresponding author email: [email protected]

Porang are known as plants that have high economic value. This plant was produced tubers which
are widely used by industry as paper adhesives, cotton fabric paint, wool and the flour can be used
as a substitute for agar. The Increased of this plant cultivation produced leaves which were not
utilized. The purpose of this study was to determine the potential of porang leaves as antioxidant
and antibacterial against Escherichia coli, Salmonella thypii. Porang leaves extract was determine
as antioxidant used DPPH method with quercetin as a standard and extract concentrations of 100
ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm. Antibacterial test using disc diffusion method with
positive control of ciprofloxacin 5 μg/disk, negative control of DMSO 10% and extract
concentration of 10%, 20%, 40%, 80% and 100%. The results of this study indicate that porang
leaves have potential as antioxidants and antibacterials. Porang leaves extract had the greatest
antibacterial activity against Salmonella thypii at concentration of 100% with inhibition zone of
11.10 mm in the strong category. While the Escherichia coli bacteria at concentration of 100%
was 8.10 mm in the medium category. Porang leaves extract has weak antioxidant activity with
IC50 value of 466.07 ppm compared to the standard IC50 value of quercetin was 11.23 ppm in the
very strong category.

Keywords: Antibacterial, antioxidant, Porang leaves, Escherichia coli, Salmonella thypii

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 57

OP 34

In Silico Study Α-Mangostin Compounds As Inhibitors Of

Glycogen Synthase Kinase 3β (Gsk 3β) Receptors In Breast
Cancer
Rizky Dwi Handayani, Citra Dewi, Dwi Syah Fitra Ramadhan
Pharmacy Departement, Mandala Waluya University
*Corresponding author email: [email protected]

Breast cancer forms from breast cells that grow and expand uncontrollably in the lining of the
lobules and ducts. One of the causes of breast cancer is due to overexpression of Glycogen
Synthase Kinase 3β (GSK 3β) through the wnt/β-catenin pathway so that β-catenin is not
phosphorylated causing β-catenin to accumulate in the cytoplasm and move to the nucleus and
activate TCF/LEF, which will trigger the occurrence of cancer. One of the compounds that have
activity as the anticancer breast is α-mangostin. This study aimed to determine the α-mangostin
compound can act as an inhibitor of the GSK 3β receptor in breast cancer. The method in this study
was in silico through molecular docking at the GSK 3β receptor (PDB code: 1Q3D, 1PYX, 4ACC,
3GB2, 4PTE, 1Q5K) and α-mangostin using Autodock Tools 4.2 software and BIOVIA discovery
studio 2019. The molecular docking results obtained showed that α-mangostin compound had a
lower bond energy value and inhibition constant than the natural ligand (comparison) in PDB code
1Q3D (-8.76 kcal/mol and 376.96 μM), 4ACC (-7.66 kcal/mol and 2.44 μM) and 1Q5K (-6.49
kcal/mol and 17.55 μM). The interaction results of the amino acid residues of the α-mangostin
compound had similarities with the three natural ligands with a percentage range of 66-84%. This
showed that the compound α-mangostin could be used as a candidate for breast cancer drugs.

Keywords : α-Mangostin, Breast Cancer, GSK 3β Receptor, In Silico

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 58

OP 35

In silico study of compounds from Crocus sativus l. Towards

glycogen synthase kinase-3 beta (gsk-3β) receptors as
antidiabetic
Dian Afriana,Citra Dewi, Dwi Syah Fitra Ramadhan
Pharmacy Departement, Mandala Waluya University
*Corresponding author email: [email protected]

Crocus sativus L. is a plant of the Iridaceae genus which contains flavonoid, terpenoid, and
carotenoid compounds known to be potential antidiabetic candidates. In this case, Glycogen
Synthase Kinase-3 Beta (GSK-3β) is a protein kinase that affects insulin signaling pathways,
causing insulin resistance. The purpose of this study was to determine the molecular interaction of
compounds from Crocus sativus L. to the GSK-3β receptor as an antidiabetic. This study
employed in silico through molecular docking at the GSK-3β receptor with PDB code: 4PTE and
compounds from Crocus sativus L. using Autodock vina software and visualization using BIOVIA
discovery studio 2019. The results obtained were 9 compounds that have better affinity than natural
ligands starting from the value range of -9 kcal/mol to -11.5 kcal/mol. and supported by the
similarity of 50% amino acid interaction with the best compound, namely kaempferol-7-O-
glucosidem. This had a similar interaction with natural ligands by 85%. This shows that
compounds from Crocus sativus L. can be candidates for antidiabetic drugs.

Keywords : In silico, Saffron, GSK-3β, antidiabetic.

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 59

OP 36

Potential Components of Gedi Leaves (Abelmoschus

manihot (L.) Medik) as XO Enzyme Inhibitors Causing Gout:
an in silico Approach
Faruk Jayanto Kelutur1,2*, Wa Ode Ida Fitriah3
1
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Pattimura University, Street Ir. M.
Putuhena, Poka, Ambon Bay, Ambon City, Maluku 97233.
2
Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Padjadjaran University,
Hegarmanah, Jatinangor, Sumedang Regency, West Java 45363.
3
Department of Pharmacy, Faculty of Science and Technology, Mandala Waluya University, Poasia, Kendari City,
Southeast Sulawesi 93232.
*
Corresponding Author: [email protected]

Ethnobotanically, the people of Maluku often use gedi leaves as a medicinal ingredient to treat
gout, but this has not been done scientifically. Gedi plant has been reported to contain secondary
metabolites of flavonoids, steroids, alkaloids, and phenolic compounds with pharmacological
activities of anti-inflammatory, anti-oxidant, anti-obesity, analgesic, and anti-diabetic. This
research was based on structure-based (molecular docking) using Autodock Tools 4.2, then
visualized with Discovery Studio 2016 Client®. While prediction of pharmacokinetic properties,
oral administration, and acute oral toxicity with pre-ADMET, Lipinski's rule of five, and ProTox-
II. The results showed that the compound myricetin 3-O-beta-D-glucopyranoside (a flavonoid
component) has the potential to inhibit the xanthine oxidase (XO) enzyme when docked with
binding affinity values (ΔG, kcal/mol and Ki, nM) of -10.43; 22.78 compared to allopurinol (-5.46;
98760), Thr1010 and Arg880 were crucial amino acids that play a role when binding. ADME-Tox
predictions show good disposition and metabolism in the liver as a drug but were mutagenic. In
addition, the value of lipophilicity (log P) -0.053 indicates good absorption and permeation as a
drug candidate, while the LD50 was 1000 mg/kg. Thus, gedi leaves can be gout, but structural
modifications are needed.

Keywords: Maluku, gedi leaves, XO enzyme, in silico

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 60

OP 37

Collagen Thickness and Density in BALB/c Mice Exposed to

UVB Light After Using Siam Weeds Cream (Chromolaena
odorata L.)
Karlina Amir Tahir1, Upik A. Miskad2, Khairuddin Djawad3, Sartini4, Maulita Indrisari5, Arief
Budiyanto6, Ahmad Lalo7, Syamsuri Syakri1, Asrul Ismail1, Khaerani1
1)
Department of Pharmacy, Faculty of Medicine and Health Science, UIN Alauddin Makassar, Makassar, Indonesia
2)
Department of Anatomical Pathology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
3)
Department of Dermatology and Gender, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
4)
Department of Pharmacy and Science, Faculty of Pharmacy, Universitas Hasanuddin, Makassar, Indonesia
5)
STIFA, Makassar, Indonesia
6)
Department of Dermatology and Gender, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
7)
National Agency of Drug and Food Control, Makassar, Indonesia

Corresponding author : [email protected]

Ultraviolet B (UVB) radiation can cause damage to the skin's dermis layer, which can lead to collagen
fragmentation. Damage to collagen generates wrinkles, which are a sign of aging. The purpose of this study
was to determine how Siam Weeds cream (Chromolaena Odorata L.) altered the thickness and density of
collagen in the skin of BALB/c mice after UVB exposure. There were four groups of 24 BALB/c mice
altogether: normal control, negative control (Cream Base with UVB), positive control (UVB), and treatment
(C. odorata cream and UVB). Just before and after the mice were exposed to UVB at 500 mJ/cm 2, the Siam
Weeds cream formulation was applied at a concentration of 1%. Mice were slaughtered, and a back skin
excisional biopsy was conducted to analyze collagen. The treatment group differed significantly both in
thickness and density of collagen against the positive control group and the negative control group (p<0.05)
and There was no statistically significant difference between the treatment and normal control groups
(p<0.05). A 1% dosage of C. odorata cream can improve the thickness and density of collagen in UVB-
exposed mice's skin.

Keywords: Chromolaena odorata L. Cream, Collagen, UVB

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 61

OP 38

In silico study phenolic compounds contained in rosella

flower (hibiscus sabdariffa l.) As CTLA-4 receptor inhibitors
Lingga Nurhayati, Wa Ode Yuliastri, Dwi Syah Fitra Ramadhan
Department of Pharmacy, Universitas Mandala Waluya, Kendari, Indonesia

*Corresponding author : [email protected]

Cancer is a dangerous disease that can cause death. It is proved that 9,6 million deaths were
recorded in the world in 2018 and 234,5 thousand deaths in Indonesia in 2020. Thus, it is necessary
to develop effective and safer cancer therapies. One of the cancer therapies that is currently
developing rapidly is immunotherapy. Immunotherapy works by increasing the body’s immune
system to fight cancer, where one approach is known as immune blockade checkpoints (blocade
immune inhibition pathway activated by cancer cells). One of these immune checkpoint receptors
is CTLA-4. CTLA-4 receptors play a role in regulating T cell proliferation in the immune system.
The inhibition of CTLA-4 receptors can improve the function of the immune system in fighting
cancer cells. One of the compound that can block immune checkpoints is a phenolic compound
commonly found in the Rosella flower plant (Hibiscus sabdariffa L.). this study was conducted to
determine the potential of phenolic compounds as CTLA-4 receptor vy looking at their affinity
and molecular interactions in silico. This study was analytical research that was done
computationally. The population was all 3D compound structures in the 3D KNNApSAcK site.
The sample used was the 3D structure of phenolic compounds in the Rosella flower (Hibiscus
sabdariffa L.). This study was conducted using the molecular docking method. The results showed
that 10 of the 13 phenolic compound in the Rosella flower (Hibiscus sabdariffa L.) had an affinity
for the CTLA-4 reseptor in silico. Quercetin is a compound that has the best affinity for amino
acids at the CTLA-4 receptor.

Keywords: Immune Checkpoint, CTLA-4, Phenolic, Rosella, Molecular Docking

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 62

OP 39

The Relationship Between Pharmaceutical Service Quality,

Patient Satisfaction And Pharmacy Installation Images At The
Regional General Hospital Of Kendari City : A Comparative
Study Between Outpatient And Inpatient
Juliana Baco, Sunarsih, Tasnim
Mandala waluya University
*Corresponding author : [email protected]

Pharmaceutical services improve the patient's quality of life. This study aimed to examine the
effect of inpatient and outpatient pharmaceutical services, patient satisfaction and pharmacy
installation images at the Regional General Hospital of Kendari City. The samples involved in
this study were 180 respondents, consisting of 99 outpatients and 81 inpatients. The data were
analyzed using the chi-square test to find out the relationship between variables. The Cramer's test
was used to determine the magnitude of the effect between variables. In addition, the man-Whitney
test was used to examine differences in perceptions between inpatients and outpatients. The results
showed that based on the chi-square test, there was a relationship between outpatient pharmacy
services and outpatient satisfaction, there was a relationship between outpatient pharmacy services
and outpatient pharmacy installation images, there was a relationship between outpatient
satisfaction and outpatient installation images, there was no relationship between inpatient
pharmaceutical services and inpatient satisfaction, there was a relationship between inpatient
pharmacy services and outpatient pharmacy installation images, and there was no relationship
between inpatient satisfaction and outpatient pharmacy installation images. Based on the results
of the man-Whitney test, the inpatients and outpatients had different perceptions on service
variables, satisfaction, and pharmacy installation images. This study hopes that the quality of
pharmaceutical services can be improved in accordance with pharmaceutical service standards,
minimize waiting times, complete drug availability, optimize drug information, education services
to patients, and revitalize waiting rooms.

Keywords: Services, satisfactions, images, inpatients, outpatients

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 63

PP 01

Effectiveness Level of Vitamin C Use Against Kidney Damage

in Mice (Mus musculus L) with Injection Treatment
Dwintha Lestari1, Sindi Widia1 , Titian Daru Asmara Tugon2, Nanda Raudhatil Jannah3, Fauziah
Ningrum Syaputri4
Faculity Of Pharmacy, Universitas Muhammadiyah Bandung, Indonesia
*Corresponding author : [email protected]

Vitamin C is a type of water-soluble vitamin and has an important role in warding off various
diseases. This vitamin is also known by the chemical name of its main form, namely ascorbic acid.
Vitamin C belongs to the group of antioxidant vitamins that are able to ward off various free
radicals. This study aims to see the effect of using vitamin C with variations in different doses and
consumed continuously for a long time which can have an impact on kidney damage in mice (Mus
musculus L) with the use of variations in the dose used. The method in this study was experimental
which included preparation of test animals, pre-treatment stage, treatment stage, post-treatment
stage, then administration of vitamin C and data analysis using SPSS (Statistical Product and
Service Solution).In this study, 20 mice were used which were divided into 5 groups. The first
group, namely the group without treatment. The second group, namely the control, was given 1 ml
of distilled water. The third group, namely the group with a small dose of 0.012 mg/ BW. The
fourth group with a moderate dose of 0.024 mg/BW andthe fifth group with a large dose of 0.036
mg/ BW. The dosing of vitamin C lasted for one month and then euthanasia and kidney collection
were carried out macroscopically. The results showed that the administration of vitamin C had a
damaging effect on the kidneys according to the level of the dose given for one month. The heaviest
kidney damage was experienced by the third and fourth groups with a dose of 0.024 mg/ BW and
a dose of 0.036 mg/ BW.

Keywords: Dose variation, Kidney, Mice (Mus musculus L), Vitamin C.

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 64

PP 02

Astaxanthin Gen Expression In Haematococcus pluvialis

With NaCl Induction And High Light Intensity
Soni Muhsinin1, Widhya Aligita1, Tina Rostinawati2, Jutti Levita3,
1
Faculty of Phramacy, Bhakti Kencana University, Jl. Soekarno Hatta N0.754, Bandung, West Java, Indonesia
40286.
2
Department of Biology Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang
km.21, Sumedang, West Java, Indonesia 45363.
3
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya
Bandung-Sumedang km.21, Sumedang, West Java, Indonesia 45363.

*Corresponding author. Email: [email protected]

H. pluvialis is a unicellular microalga capable of producing astaxanthin up to 5% dry weight under

stress conditions. Astaxanthin has the main activity as an antioxidant, so that it is widely used in
various fields of life, especially health. Therefore, efforts are needed to increase astaxanthin levels
in H. pluvialis. Some efforts that can be made are the addition of chemical and physical induction
and a combination of both. As the productivity of astaxanthin increases, it affects the genes
involved in astaxanthin biosynthesis. These levels are related to the purpose of this increased
research, namely to determine the effect of gene expression (crtR-b) β-carotene hydroxylase on
astaxanthin production in H. pluvialis microalgae induced by the addition of NaCl and high light
intensity using the primary method of gel Polymerase Chain Reaction (PCR). H. pluvialis was
cultivated using Guillard media for seven days. On day 8, the culture was given 0.8% NaCl
induction treatment, exposed to 6800 lux light, a combination of the two, and without any treatment
as a negative control. The extraction method used is maceration using DMSO (Dimethyl
Sulfoxide), then measured astaxanthin levels using a spectrophotometer at λ 490 nm. The
measurement of astaxanthin levels showed the highest levels produced by culture with 0.8% NaCl
induction as much as 0,311 mg/L. The method used for gene amplification was gel-based PCR
with the highest AUC value of 9736,333 produced by culture with 0.8% NaCl induction.

Keywords: Asataxnthin, crtR-b gene, H. pluvialis, chemical and physical induction, PCR gel base

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 65

PP 03

The Analgesic and Antipyretic Activity Test of Syzigium

cumini (L). Skeel Leaves Ethanol Extract in Mice
(Mus musclus)
Suwahyuni Mus*, Sitti Rahimah, Fadhillah Maryam
Sekolah Tinggi Ilmu Farmasi Makassar
*Correspondencing author :[email protected]

Analgesic-antipyretic is a compound used to reduce pain and fever. This study aims to determine
whether the ethanol extract of Syzigium cumini leaves has analgesic and antipyretic activity in mice
(Mus musculus) induced by acetic acid and peptone. The method used was the Writhing method
(stretching method) with acetic acid induction for analgesics and the rectal temperature
measurement method for antipyretic testing. This research was started by extracting jamblang
leaves (Syzigium cumini (L.) Skeels) with 70% ethanol solvent by maceration. Jamblang leaf
extract was then tested in 5 groups, positive control group given paracetamol for antipyretic testing
and mefenamic acid for analgesic testing, negative control group (Na.CMC suspension), a dose of
6 mg/Kg, 9 mg/Kg and 12 mg/ Kg of body weight. The results indicate that the ethanol extract of
Syzigium cumini leaves has analgesic and antipyretic activity at all treatment doses and the most
effect is at a dose of 6 mg/KgBW.

Keywords: Syzigium cumini (L) Skeels, Mice, Analgesic, Antipyretic

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 66

PP 04

Determination of Total Phenolic Concentration of Ethanol

Extract of Chrysophyllum cainito L. Using UV-Vis
Spectrophotometry
Fadillah Maryam*, Imrawati, Suwahyuni Mus

Sekolah Tinggi Ilmu Farmasi Makassar

*Corresponding author : [email protected]

Sawo durian (Chrysophyllum cainito L.) is widely used for various diseases including as
antidiabetic mellitus, antirheumatic, reducing inflammation in the throat and lungs, and as an
antioxidant. This study aims to determine the total phenolic content of the ethanol extract of
C.cainito leaves extracted using the maceration method with 70% ethanol solvent with the
yield of 20.828%. Total phenolic content was determined using UV-Vis spectrophotometry using
the Follin-Ciocalteau method and gallic acid was used as a comparison. Measured at = 722 nm,
with linear regression equation y=0.099x-0.0403 and the value of r= 0.9999. The total phenolic
content obtained was 20.55 mg GAE/g.

Keywords : Chrysophyllum cainito, total phenolic, UV-Vis Spectrophotometry

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 67

PP 05

Physical and Antioxidant Stability of Lotion Nanoparticles of

Soursop (Annona muricata L.) Ethanolic Extract
Malinda Prihantini1, M Fatchur Rochman1, Anisa Rizki Amelia2, Tsabita Udzkiya Zulfa2,
1
Department of Pharmaceutical and Technology, Faculty of Pharmacy, Universitas Wahid Hasyim
2
Undergraduate Programme, Faculty of Pharmacy, Universitas Wahid Hasyim
*Corresponding author. [email protected]

Soursop leaf ethanol extract contains flavonoids as antioxidants which are unstable to
environmental influences. The nanoparticle system with chitosan coating, encapsulated the extract
and increased its stability in the preparation. Lotion is a preparation that allows rapid, easily spread
and dries immediately after application. The aim of this study is to determine the physical stability
and antioxidant activity of lotion nanoparticles from the ethanolic extract of soursop leaves
(Annona muricata L.). Soursop leaf ethanol extract was obtained by maceration using 70%
ethanol. Lotion formulation with various concentrations of ethanolic extract of soursop leaf
nanoparticles F1 (100 mg); F2 (200 mg); and F3 (300 mg). Storage in the physical stability test
using the cycling test method at a temperature of 4°C for 24 hours, and a temperature of 40°C RH
70% ± 5% for 24 hours (1 cycle). The test was carried out for 6 cycles and followed with
organoleptic parameters, homogeneity, adhesion, spreadability, viscosity, pH, and separation
evaluation. Antioxidant activity was determined using the DPPH method. The test data of
dispersion, adhesion, viscosity and antioxidant activity were analyzed by paired sample t-test,
while organoleptic, homogeneity and pH were analyzed descriptively. The lotion of nanoparticles
of soursop leaf ethanol extract was white, semi-solid, had a distinctive odor and was suitable for
skin pH and did not undergo separation after mechanical testing. The results of the physical
stability test of the lotion nanoparticles of soursop leaf ethanol extract after the cycling test showed
that the spreadability, adhesion and viscosity of the three formulas were stable, while the
antioxidant activity of F2 was stable, but F1 and F3 were unstable.

Keywords: antioxidant, lotion, nanoparticles, soursop, stability

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 68

PP 06

Formulation Of Talas Leaf (Colocasia Esculenta) Emulgel As

A Candidate Of Antibacterial Causes Of Bulbs
Maria Ulfa*, Radia Reski, Muthiah Ridhayanah
Sekolah Tinggi Ilmu Farmasi Makassar
*Correspondencing author : [email protected]

One of the plants that are used as a boil medicine by the community especially the people of Papua
is taro leaves (Colocasia esculenta). Taro leaves have antibacterial activity against bacteria that
cause boils (S. aureus) with a concentration of 5%. This research aims to obtain a taro leaf emulgel
preparation that is physically and determine the effectiveness of taro leaf emulgel against bacteria
that cause boil. Taro leaf extract with a concentration of 5% was made into an emulgel preparation
using gelling agent carbopol 940. gelling agent carbopol 940 with various concentrations of 1%,
1.5% and 2%. Then each formula was tested for its antibacterial effectiveness by the good diffusion
method against boil causing bacteria with positive control of gentamicin cream. Data analysis was
carried out by calculating the average of the inhibition zones for each concentration. Taro leaf
emulgel formula (Colocasia esculenta) is stable physically is FII using 1.5% gelling agent.
Effectiveness test taro leaf emulgel formula against boil causing bacteria (S. aureus) namely FI,
FII, and FIII with a resistance of 18.24, respectively, 18.99, and 17.58 in the strong category.

Keywords: Taro leaves, emulgel, antibacterial

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 69

PP 07

In Silico Study : The Blue Butterfly Pea Flower (Clitoria

ternatea L.) Potency for Sars-Cov-2 Main Protease Inhihibor
Safira Yulita Fazadini
Universitas Surabaya

*Corresponding author : [email protected]

The number of COVID-19 cases in the world is still increasing. As of November 2021, almost 263
million people in the world have been confirmed to have COVID-19 with a fairly high mortality
rate of 2% (WHO, 2021). In Indonesia, around 4.2 million people have been confirmed positive
for COVID-19 and have caused 3.4% of deaths, which is a higher number than the number of
deaths in the world (Kemenkes RI, 2020). Specific drugs officially approved for the treatment of
COVID-19 are still in development. Several vaccines have been circulated, but their effectiveness
is still in the development stage until now. Research on herbs use for the treatment of COVID-19
is also ongoing. In Indonesia, the use of herbs as a treatment is still widely used. Blue Butterfly
Flower (Clitoria ternatea L.) is a plant that has not been widely researched. This poster will discuss
the physico-chemical properties and drug-likeness properties of compounds contained in Blue
Butterfly Flower. The Compounds that already qualify for druglikeness properties will be seen for
their interactions with the SARS-CoVMPro receptor (PDB: 7AHA) using the Autodock-Vina
program. Blue Butterfly flower is promising candidate for efficacious food application as a for
Sars-Cov-2 Main Protease inhibitor because pharmacotherapeutic properties, safety and
effectiveness.

Keywords: covid-19, in silico, main protease, bunga telang, Blue Butterfly Flower (Clitoria
ternatea L.)

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 70

PP 08

Potential Antihyperlipidemic Activities From Purified Extract

Of Lawsonia Inermis Leaves In Hyperlipidemic Mice
Mus Ifaya*, Melda Azis Sri Wulandari, Silviana Hasanuddin, Juliana Baco
Department Of Pharmacy, Faculty of Science and Technology, Universitas Mandala Waluya, Southeast Sulawesi,
Indonesia
*e-mail addres of corresponding author: [email protected]

Hyperlipidemia is a lipid metabolism disorder characterized by an increase in one or more total

cholesterol, Low Density Lipoprotein (LDL), or triglycerides or a decrease in High Density
Lipoprotein (HDL). Lawsonia inermis leaves is an Indonesian medicinal plant that used as
traditional medicine. Phenols and flavanoids are the most commonly found active compounds.
This research was conducted to determine potential antihyperlipidemic activity of purified extract
L.inermis leaves in mice (Mus musculus) in reducing blood cholesterol levels. The method were
including of maceration, purification using liquid- liquid extraction and antihyperlipidemic
activity test to reduce blood cholesterol levels in mice. Mice were divided into 6 groups. Group I
(normal control), groups II (Simvastatin 10 mg/kg bw in the positive control), groups III (sodium
CMC 0.5% in the negative control, groups IV-VI (Purified extract at dose 200, 400, and 800
mg/kg bw).The result showed that there were 6 of groups (I-VII) antihyperlipidemic activity test
of purified extract L.inermis leaves at 200, 400 and 800 mg/kg bw shown to reduce blood
cholesterol levels in mice were 13%, 18% and 24% , respectively.

Keywords: Lawsonia inermis, purified extract, hyperlipidemia, total cholesterol

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice
 71

PP 09

Comparison of Extraction Methods to Antioxidant Activity

and Determination of Total Flavonoid and Phenolic Levels in
Red Leeks (Allium ascalonicum .L)
Maria Ulfah1*, Dwi Yuni Mufarikhah1, Ranni Puji Astutik1, Mutmainnah1
Pharmacy faculty, Wahid Hasyim University

Extraction methods can affect the content of chemical compounds and total phenolic flavonoid
levels in leek extract (Allium ascalonicum. L). The study's goal was to compare variations in
extraction methods against antioxidant activity as well as determination of total flavonoid and
phenolic levels of red leaf extract. The extraction methods used are maceration, soxletation,
percolation, reflux using a 70% ethanol solvent. The extract was qualitatively tested for
phytochemical screening and measurement of the extract's antioxidant activity using the DPPH
(2,2-diphenyl-1picrylhydrazyl) method with a comparison of vitamin C λ 516.5 nm and
determination of total flavonoid and phenolic compound levels by comparing quercetin λ 430 nm
and λ 743 nm gallic acid with UV-Vis Spectrophotometry. IC50 values as well as flavonoid and
phenolic levels were analyzed with the One Way Anova and T independent tests. The results
showed that the leek extract contained flavonoids, phenolics, tannins, saponins and alkaloids with
total flavonoid levels with percolation, reflux, maceration, and sokletation methods of 3,745±0.02;
3,528±0.02; 2,935±0.02 and 1.47±0.02 mgQE/g extract. As for the total phenolic level of
179,708±0.42; 137,625±0.42; 83,875±0.42 and 69,708±0.42 mgGA/g extract. IC50 values of the
four methods of percolation, reflux, maceration, and sokletation of 88.12±0.78; 95.09±1.11;
99.39±0.49: 105.08±0.39 ppm. The results of an independent t-test analysis showed significant
differences in flavonoid and phenolic levels of total leek extract (Allium ascalonicum). L) among
the various extraction methods.

Keywords: Antioxidants, DPPH, Maceration, Percolation, Refluk, Soxletation, Phenolic, Total

Flavonoids

1St MWICPSP
The 1st Mandala Waluya International Conference on Pharmaceutical Science and Practice