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Hstcyt Part2

Notes

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Rafaela Mae Javellana Galeste
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15 views

Hstcyt Part2

Notes

Uploaded by

Rafaela Mae Javellana Galeste
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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FACTORS TO BE CONSIDERED IN CHOOSING A (putrefaction(overgrowth of microorgamism) and

METHOD autolysis(cell destruction)

1. Structural and chemical components to be 2. PRESERVED TISSUE EXAMINATION- use of


studied fixative)

2. Nature and amount of sample to be evaluated Initial Steps in Tissue Processing

3. The need to provide an immediate diagnosis A. Specimen Accessioning/Identification(receiving


specimen)

➢ Performed by the medical technologist


METHODS OF PREPARATION AND EXAMINATION
➢ Check label and request form
A. Diagnostic Laboratories
➢ The specimen is given a label (numeric or alpha
1. FRESH TISSUE EXAMINATION -numeric) which allows easy
accessioning/identification
➢ No fixative required
➢ Request form should have a provisional
➢ Examined using a Brightfield or Phase-Contrast
diagnosis and brief clinical details
microscope

➢ Stained with supravital or differential dyes


B. Gross Examination and Sampling
Suppravital dyes:- living cells
➢ Performed by the pathologist
 Brilliant cresyl blue
➢ Describing the sample macroscopically
 New methylene blue
➢ Weight and dimensions of the sample are
Differential dyes: 2 structure of cells (nucleus or
determined
cytoplasm)
C. Tissue Processing
 Eosin- cytoplasm
➢ Fixation
 Methylene blue- nucleus
➢ Dehydration
 Crystal violet- nucleus
➢ Clearing
 Carbol cros- cytoplasm
➢ Infiltration- impregnation

➢ Embedding- blocking, cascade


ADVANTAGES
➢ Sectioning (+ Floating, Fishing-out, Drying)-
✓ Observation of physiologic processes or microtomy
protoplasmic activities (motion, mitosis,
phagocytosis(cell engulfment) and ➢ Staining
pinocytosis( cell drinking or up taking of cell by
water) ➢ Mounting

✓ Relatively simple and easy to perform ➢ Labelling

DISADVANTAGES

✓ Limited use (it will deteriorate)

✓ Liable to develop changes observed after death


B. Research Laboratories lung cancer

1. MICROINCINERATION-heat SREAKING- accomplished using applicator stick or


platinum loop direct or zigzag steak.
✓ Used to locate the presence and position of
mineral elements in the tissue SPREADING- utilizes directed portion of material,
thick portion is transfered and the mucus stands
✓ Two duplicate sections of alcohol-fixed tissues apart with an applicator stick
2. AUTORADIOGRAPHY PULL APART- a drop of sample is placed on one
slide and disperses upon the placement on the
✓ Injection of radioactive isotopes into organs--
other side. Two slides pull apart in opposite
isotope labelling
directions uninterrupted may be fresh or vital
Fix→Section→Mount + Photographic Emulsion stains ex. Vaginal smears
(Ag Halide)→Stain
TOUCH PREPARATION
✓ Determines the relationship and location of the
isotopes and cells to be studied
Fe De Ca? Important Event ng BTS Sa MOA Lang
✓ Provides qualitative and quantitative informatio
Fe- fixation- (preservation of tissue)

De- Dehydration(removal of water from tissue)


Decalcification (optional step only for bone
specimen

Ca- clearing- make the tissue transparent

Important- Impregnation- infiltration-(fill up holes


of tissue)(optional step, if frozen tissue no need to
infiltrate)

Event- embedding

B- blocking

T- trimming

S- sectioning- produce tissue ribbons

Sa- staining

TEASING OR DISSOCIATION - dissection of MOA- mounting- putting coverslip


specimen of a needle. Dissect it in to
thinnest.permits the cell to be examine in a living L- labelling
state. MB

SQUASH OR CRASHING

- done in less than 1mm small tissue. Put in a


stationary slide and cover with another slide crash
or squeeze.MB

SMEARING- can be made permanent by fixing the


sample . Required thick secretion such as serous
fluid, concentration sputum and enzymatic lavadge.
For cancer diagnosis. Example cervical cancer ,

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