IVIS | Lumina II

Quantitative Fluorescent
and Bioluminescent Imaging
The IVIS Lumina II from Caliper Life Sciences provides an expandable,
sensitive imaging system that is easy to use for both fluorescent and
bioluminescent imaging in vivo. The system includes a highly sensitive
CCD camera, light-tight imaging chamber and complete automation
and analysis capabilities. As the leading optical imaging platform for
in vivo analysis, IVIS systems include a range of practical accessories
developed through experience in research laboratories worldwide.
IVIS LUMINA II

In Vivo Molecular Imaging

Quantitative Flexible Expandable
An adjustable field of view from 5 – 12.5 cm and an optional 24cm lens allows imaging of up to 5 mice or 2
medium size rats. The Lumina II can also accommodate Petri dishes or micro-titer plates for in vitro imaging. The
system includes premium animal handling features such as a heated stage, gas anesthesia connections and ECG
monitoring.
High resolution, sharp cut-off filters are simply interchangeable to achieve the highest performance, sensitivity
and spectral unmixing in fluorescence imaging.

Superior Imaging Results

The IVIS Lumina II is capable of imaging both fluorescent and bioluminescent reporters. The system is equipped
with up to 21 filter sets that can be used to image reporters that emit from green to near-infrared. Superior
spectral unmixing can be achieved by Lumina II’s optional high resolution short cut off filters. Absolute calibration
affords you consistent and reproducible results independent of magnification, filter selection from one instrument
to any another IVIS instrument within an organization or around the world. The Living Image software yields
high-quality, reproducible, quantitative results incorporating instrument calibration, background subtraction and
the image algorithms.

Customize the IVIS Lumina II with your own Filter Combinations

Standard High Resolution
Fluorophores Emission Filter Options
Excitation Filter Set (Built-in)
*500 Series
GFP, YFP and PKH26
500, 520, 540, 560, 580, 600 and 620
Cy 5.5, DsRed, dTomato *600 Series
and XenoFluor 680 430, 465, 500, 580, 600, 620, 640, 660, 680 and 700
535, 570, 605,
Indocyanine Green and 640, 675, 710, 745 *700 Series
XenoFluor 750 720, 740, 760, 780, 800, 820, and 840
Multiple Fluorophores Spanning Standard Emission Filter Set
500-900 nm Broad Imaging Solution 515-575, 575-650, 695-770, 810-875
* Median wavelength band path 20nm on emission filters

Field of View
Ex Vivo In Vivo

Standard Lens FOV New XFOV-24 Upgrade

The IVIS Lumina II Imaging System provides 5 fields of view.
 IVIS LUMINA II

Imaging Results - Living Image Software with High Resolution Filters

Triple Reporter Imaging Spectral Unmixing of Xenofluor 680/750

Thigh Infection with Klebsiella pneumoniae expressing Subcutaneous injections of 1014 molecules of XenoFluor 680 (scruff)
luxCDABE with optimized GFP or RFP. Approximately 108 and 1014 molecules of XenoFluor 750 (lower dorsal region) 605nm
CFU per thigh. Courtesy of the University of Glasgow. excitation filter.

Dual Reporter Imaging - High Resolution

Ex Vivo Applications
Bacterial luc (500nm) and GFAP (620nm)
brain imaging from mice with Pneumococcal
Meningitis. Ex Vivo Kadurugamuwa et al.,
Infection and Immunity, 2005.

Living Image Software

with IVIS Lumina II System
The wide range of IVIS system instru-
ment settings, combined with abso-
lute calibration of each setting, allows
users to track signals during longitu-
dinal studies that vary by many orders
of magnitude. In this drug study, tu-
mor signals vary by three orders of
magnitude during the course of a 35
day experiment. The capability of Liv-
ing Image Software makes this type of
analysis simple for the user in both flu-
orescent and bioluminescent modes.
Inside the IVIS Lumina II
CCD Camera
• The IVIS Lumina II CCD is 13 x 13 mm square, with 1024 x 1024 pixels 13 micron in width, yields higher imaging resolution
• Back-thinned, back-illuminated grade 1 CCD provides high quantum efficiency over the entire visible to near-infrared spectrum
• 16 bit digitizer delivers broad dynamic range
• The CCD is thermoelectrically (Peltier) cooled to -90 ºC
ensuring low dark current and low noise

Imaging Chamber
• Light-tight imaging chamber
• High light collection lens, f /0.95 – f/16
• Optional 24cm FOV lens attachment
• 8 position emission filter wheels
• Replaceable filter wheel upgrades for high resolution
fluorescence imaging
• LED lamps for photographic images
• Heated stage to maintain optimum body temperature
• Motor controlled stage, filter wheel, lens position, and f-stop
• Optional integrated ECG monitoring system

Integrated Gas Anesthesia

• Gas anesthesia ports and 5 position manifold within imaging
chamber allow anesthesia to be maintained during imaging sessions

IMAGING SYSTEM COMPONENTS SPECIFICATIONS

Camera Sensor Back-thinned, back-illuminated, cooled Grade 1 CCD
CCD Size 1.3 x 1.3 cm
Imaging Pixels 1024 x 1024
Quantum Efficiency >85% at 500 – 700 nm, >30% at 400 – 900 nm
Pixel Size 13 microns
Min. Detectable Radiance 100 photons/s/sr/cm2
Min. Field of View (FOV) 5 x 5 cm
Max. Field of View (FOV) 12.5 x 12.5 cm (optional 24 x 24 cm)
Min. Image Pixel Resolution 50 microns
Read Noise < 3 electrons for bin=1,2, 4; < 5 electrons for bin=8, 16
Dark Current (Typical) <120 electrons/s/cm2; or 2 x 10-4 electrons/s/pixel
Min. Detectable Radiance 100 photons/s/sr/cm2
Lens f/.95 – f/16, 50 mm
Fluorescence Capability Standard
Excitation Fluorescence Filters 10
Emission Fluorescence Filters 4 standard (Optional 3 sets of 7 high resolution filters)
Fluor. Bkg. Subtraction Filters Included
CCD Operating Temp .-90 °C
Imaging System Space Requirement 48 x 71 x 104 cm (W x D x H)
Imaging Chamber Interior Dimension 43 x 38 x 43 cm (W x D x H)
Power Requirements 6A at 120V
Stage Temperature 20 – 40 °C
Computer (Minimum specifications) 2.8 GHz, 1 GB RAM, RW CD/DVD, 80 GB HD, 20” flat screen monitor
Living Image Software 1 acquisition copy and 4 analysis copies of Living Image software 3.0.4x and higher

Front cover images:

Top Left: Orthotopic prostate tumor (PC-3) labeled with GFP (Courtesy of AntiCancer)
Middle: Sample of the Lumina ECG monitoring system,
Bottom: Subcutaneous injections of XenoFluor 680 and XenoFluor 750
Any use of products or material containing DNA sequences coding for mutant Aequorea
victoria green fluorescent protein (GFP) variants or proteins thereof requires a license
from GE Healthcare UK Limited.
Corporate Headquarters
68 Elm Street ©2008 Caliper Life Sciences, Inc. All rights reserved.
Caliper, the Caliper logo, IVIS, Lumina, Xenogen,
Hopkinton, MA 01748-1668
XenoFluor and Living Image are tradenames and/
Tel: 1.508.435.9500 or trademarks of Caliper Life Sciences, Inc. All other
Email: [email protected] names are trademarks of their respective companies.

www.caliperLS.com LUM-BR-02 Jun 08