PEAK SHAPE PERFORMANCE

UHPLC PERFORMANCE
WITH ANY LC INSTRUMENT

SUNSHELL

HARDCORE SHELL
TECHNOLOGY

GLOBAL DISTRIBUTOR
BIOTECH AB
www.biotech.se1
 PEAK SHAPE
HARDCORE
PERFORMANCE

SUNSHELL

SUPERFICIALLY EFFICIENT
POROUS SILICA

2
 *1.6 μm and 3.4 μm of core and 0.5 μm and 0.6 μm of superficially p
silica layer
*Same efficiency and high throughput as a Sub 2 μm and 3 μm par
*Same pressure as a 3 μm and 5 μm par
*Same chemistry as Sunniest technology (reference figure 1)

WHAT IS SUNSHELL? *Good peak shape for all compounds such as basic, acidic and
chela ng compounds
*High stability ( pH range for SunShell C18, 1.5 to 10)
THE NEXT GENERATION * Low breeding
0.5 µm

HARDCORE SHELL PARTICLE 0.5 µm 2.6 µm

C o re 1.6 µm C o re
2.6 µm

Secure your analysis with SunShell hardcore column technology Porous silica

Unique bonding technology combined with core shell particles gives you faster performance and more
Schematic diagram of a core shell silica particle, 2.6 µm
reliable results. The SunShell technique assures top efficiency with all kinds of LC and UHPLC systems.

FEATURES OF SUNSHELL 2.6 µm AND 5 µm

Van Deemter Equation

. 1.6 μm and 3.4 μm of core and A term : Eddy diffusion(dp is particle diameter)
B term
0.5: Longitudinal
μm diffusion
0.5 μm and 0.6 μm of superficially (Dm is diffusion coefficient)
porous silica layer. C term : Mass transfer

. Same efficiency and high throughput

as a Sub-2 μm and 3 μm particle.
2.6 μm
. Same pressure as a 3 μm and Van Deemter plot
5 μm particles. CORE 1.6 μm
s. . Same chemistry as Sunniest H
technology (reference figure below).

ell particle
. Good peak shape for all compounds
such as basic, acidic and chelating
B term
A term
C term

Superficially porous silica

compounds. POROUS SILICA
. High stability (pH range for u
orous SunShell C18, 1.5 to 10). MPa
. Low bleeding. SunShell C18 shows same efficien- 45
40 Sunniest C18-HT 2.0 µm
cy as a Sub 2 μm C18. In compar-
ison between fully porous 2.6 μm 35 Brand A C18 1.9 µm

Final TMS and core shell 2.6 μm (SunShell), 30

Back pressure, MPa

Brand B C18 1.8 µm

SunShell shows lower values for 25 Brand C C18 1.7 µm

A term, B term and C term of Van 20 Brand D C18 2.6 µm
Deemter equation. The core shell 15
SunShell C18 2.6 µm
O
Si
H
structure leads to higher perfor- 10
O
Si Si
O
mance compared with the fully 5
Column dimension: 50 x 2.1 mm
Mobile phase: Acetonitrile/water=(70/30)
Si O Si O

O O
O Si
O O Si O
Si
O O Si
Si porous structure. Temperature: 25 C o

Si Si Si O Si
Si
O OH
Si O
O
O
Si O
0
Si O
O
Si O
Si
OO
SiO
O
O

Si
O O H
Si
OO
O
Si
O
Si
OO O
Si
O O
Furthermore back pressure of 0 0,5 1 1,5
O Si O
Si

O
Si
O
Si
O
Si
O
O
O
O Si O O O
O
O O
O
Si
O
O
Si O
Si SunShell C18 is less than a half Flow rate, mL/min
O
O
O
compared to Sub-2 μm C18s. Comparison of back pressure for high throughput columns
Schematic diagram of bonding of SunShell C18
1
3
 me chemistry as *Same
*Same
Sunniest
chemistry
chemistry
technology
as
asSunniest
Sunniest
(reference
technology
technology
figure 1)
(reference
(reference figure
figure 1)
1) 0.5 µm 2.6 µm
ood peak shape*Good
*Good
for all peak
compounds
peak shape
shapeforsuch
forall
allas
compounds
compounds
basic, acidic
such
such
andas
asbasic,
basic, acidic
acidic and
and Final TMS Final
Final TM
TM
la ng compounds chela
chela ngng compounds
compounds 2.6 µm C o re 1.6 µm C o re
gh stability ( pH*High
*High
rangestability
stability
for SunShell
((pH
pHrange
range
C18, 1.5for
forSunShell
to
SunShell
10) C18,C18, 1.5
1.5 to
toSUN
10)
10)SHELL
Porous silica
ow breeding **Low Low breeding
breeding
O H OO HH

0.5 µm 0.5
0.5 µm
HOW DOES SUNSHELL WORK?
µm Schematic diagram of a core shellOsilica particle, 2.6 µm
OO
Si SiSi

0.5 µm 0.5
0.5 µm
µm 2.6 µm 2.6
2.6 µm
µm Si O O
Si Si
SiSiOOO
O
OO
SiSi SiSi
OO
Si SiSi
O OO O Si
SiSiSi OOOO SiSi SiSi
Si O Si OO SiSi

NARROW PARTICLE DISTRIBUTION

O O O O
O O Si OO O
OO O O Si
Si OO
Si Si Si O Si SiSi SiSi OSiOH
OO SiSi
Si O OO OHOH OO
Si Si SiSi SiSi
O Si SiO Si
O O OO SiSi OSi
SiOO SiSi OOSi OOO
O OO OO SiSi
O O O O H Si
OO Si Si O O Si
Si Si
Si SiSi
O O OO OO HH OOOO
C o re2.6 CCoore
1.6 µm re C o re 1.6
1.6 µm
µm CCoore
re Si
Van Deemter Equation
O O Si SiSi Si O O OO O OO O O SiSi SiSi OOOOOO OO O
6 µm 2.6 µm
µm O
Si
Si
O
Si
O Si O O O
SiOO O Si SiSiSi
O SiSi
O
O
SiSi
OOO Si
O
OO SiSi OOOO OO
SiSi
OO OO
SiSi
OO
OO
SiSi OO
SiSi
OO

O O O O OO OO OO
O O OO O OO OO OO OO
Si O SiSi OO
Porous silica Porous
Poroussilica
silica O
O
OO
OO

Schematic diagramSchematic
Schematic
of bondingdiagram
diagram
of SunShell
of
of bonding
bonding
C18 ofof SunShel
SunShe
VAN DEEMTER EQUATION
A term : Eddy diffusion(dp is particle diameter)
B term : Longitudinal diffusion
Schematic diagramSchematic
Schematic
of a core shell
diagram
diagram
silica
of
ofparticle,
aa core
core shell
shell
2.6 µmsilica
silica particle,
particle,2.6
2.6 µm
µm (Dm is diffusion coefficient)
C term : Mass transfer

18 18
18
Van Deemter Equation
Van
Van Deemter
DeemterEquation
Equation Van Deemter plot
16 16
16 Fully porous 5 µm Fully
Fullyporous
porous55µm
µm

Fully porous 3 µm Fully

Fullyporous
porous33µm
µm
A term : Eddy diffusion(dp
AAterm
term
is particle
::Eddy
Eddydiameter)
diffusion(dp
diffusion(dp isisparticle
particlediameter)
diameter) H 14 14
14
B term : Longitudinal diffusion
BBterm
term ::Longitudinal
Longitudinaldiffusion
diffusion

HETP, µm

HETP, µm
µm
12 12
12 Fully proous 1.8 µm Fully
Fullyproous
proous1.8
1.8µm
µm
(Dm is diffusion coefficient)
(Dm
(Dmisisdiffusion
diffusioncoefficient)
coefficient)

HETP,
C term : Mass transfer CCterm
term ::Mass
Mass transfer
transfer B term SunShell
10 10
10 C term 2.6 µm SunShell
SunShell2.6
2.6µm
µm
A term
8 88
Fully porous 2.6 µm Fully
Fully porous
porous 2.6
2.6 µm
µm
6 66u
Van Deemter plot Van
Van Deemter
Deemterplot
plot
4 4Column:
4 S
A TERM452
C18, 50 x 4.6 mm C18Column:
Column:C18,
C18,50
50xx4.6
4.6mm
mmC18
C18
Mobile phase: Acetonitrile/water=(60/40)
Mobile
Mobilephase: Acetonitrile/water=(60/40C
phase:Acetonitrile/water=(60/40)
2Temperature:
2 25 oC Temperature:
Temperature:2525ooCC c
H H
H 40 Sample : Naphthalene Sunniest C18-HT
Sample 2.0 µm
Sample: :Naphthalene
Naphthalene
v
0 00
Brand A C18 1.9 µm
35 0 5 00 10 55 15 10
10 15e
15
B termThe size distribution
termof a core shell
BB term porous particle, so that the space in reducing Eddy Diffusion p
30
Back pressure, MPa

Brand
Mobile phase velocity, mm/sec
Mobile
Mobile B C18velocity,
phase
phase 1.8 µm mm/sec
velocity, mm/sec
C term
(SunShell) particle is much narrower CC term
term
between the particles in the column is (multi-path diffusion) as the A term in
A term AA term
term 25 Comparison of plate
than that of a conventional totally reduced and efficiency increases by Comparison
Comparison
Van Deemter height plotsof
of plate
Brand
Equation. plate height
C C18 1.7height plots
µm plots F
20 t
u uu Brand D C18 2.6 µm
15
SunShell C18 2.6 µm
SunShell
10 C18 shows SunShell
SunShell
same efficiency
C18
C18 showsshows assame
same
a subefficiency
efficiency
2 μm as
as aa sub
sub 2
45 45
45 C18.5 In comparisonC18.
C18.
between
In
InMobile
comparison
comparison
fully porous between
between
2.6
Column dimension: 50 x 2.1 mm
μm fully
phase: Acetonitrile/water=(70/30)
fully
and porous
porous 2.6
2.6 μm
μm
Sunniest C18-HT 2.0 µm Sunniest
SunniestC18-HT core
C18-HT2.0
2.0µm
µm shell 2.6 μm (SunShell),
core
core shell
shell 2.6
2.6
SunShell
Temperature: 25 oC (SunShell),
μm
μm (SunShell),
shows lower
SunShell
SunShell shows
shows lo
lo
40 40
40 0
values0 for A term, B values
values
term
0,5
and
for
for AAC term,
term,
term 1
BB
ofterm
term
Van and
Deemter
and CC term
1,5
term of
of Van
Van De
De
35 35
35 Brand A C18 1.9 µm Brand
BrandAAC18
C181.9
1.9µm
µm
equation. The coreequation.
equation.
shell structure
The
The core
core
leads shell
shell
higher
structure
structureleads leads higher
higher
Wide particle distribution Narrow particle distribution
Flow rate, mL/min
30 30
30 performance to compare
performance
performance
with the
(Core Shell silicaD /D =1.15)to
tofully
compare
compareporous with
with
structure.
the
the fully
fully porous
porous st
s
Back pressure, MPa

pressure, MPa
MPa

Brand B C18 1.8 µm Brand

BrandBBC18
C181.8
1.8µm
µm
(Conventional silica gel D90/D10=1.50) Comparison of back pressure for 90
high 10
throughput columns
25 25
25 Brand C C18 1.7 µm Brand
BrandCCC18
C181.7 Furthermore
1.7µm
µm back pressure
Furthermore
Furthermore
of SunShell
back
back pressure
pressure
C18 isofof
less
SunShell
SunShell C18
C18 is
is l
Back pressure,

1
20 20
20 Brand D C18 2.6 µm Brand
BrandDD C18
C182.6 than
2.6µm
µm a half to compare
than
thanwith
a
a half
half
sub-2
to
to compare
compare
μm C18s.
with
with sub-2
sub-2 μm
μm C18s.
C18s.
15 15
15
Back

SunShell C18 2.6 µm SunShell

SunShellC18
C182.6
2.6µm
µm
4
10 10
10
Column dimension: 50 x 2.1 mm
Column
Columndimension:
dimension:50
50xx2.1
2.1mm
mm
 /D10=1.59 50 Sunniest, 2 μm
A term Wide particle distribution (Conventional silica gel D /D =1.50) reduces and efficiency
D : 2.63 µm
µmµm All terms
Narrow particlein Van Deemter
shell silica D /D Equation reduce.
D90: 2.65 µm D /D =1.15
D90/D10=1.59
90 10
D :D1.75
: 2.82 10
50
distribution (core =1.15) among particles in the column
90 10
90 10

The size distribution of a core

90
D :D2.01
/D µm=1.15
50 90 10 increases
reduces andby reducing Eddy
efficiency
increasesshell (SunShell) particle is much
D90: 2.31 µm
D90/D10Sunniest,
=1.32 2 μm Diffusion by reducing
(multi-path Eddy
diffusion)
CompanyAF, term
Wide particle distribution (Conventional silica gel D90/D10=1.50)
D : 1.75 µm Diffusion narrower
(multi-path than that of a
diffusion)
2 μm
SunShell,
D : 2.01 µm
10

2.6 μm as the The

Aconventional
term in Vantotally
Deemter
size distribution of aporous
core
B TERM
as the A term in Van Deemter
50
D : 1.67 µm
arison of Particle Size Distribution
10 D : 2.31 µm
Equation
Packing state of core shell and fully porous silica Flow of mobile phase
90 shell
. (SunShell) particle is much
Equation.particle, so that the space
D /D D
=1.32: 2.46 µm
: 2.09 µm
Comparison ofD Particle Size Distribution
10
D50: 2.63 µm Packing state of core shell and fully porous silica
90 10
50
D90: 2.65 Company
µm narrower than that of a
D90/D10=1.59
F, 2 μm D90: 2.82 µm
SunShell, 2.6 μm Narrow particle distribution (core shell silica D90/D10=1.15) among particles
conventional in the column
totally porous
D10: 1.67 µm
D50: 2.09 µm
D90/D10=1.15
D10: 2.46 µm Flow of mobile phase
reduces and
particle, so that the space efficiency
D90: 2.65 µm D50: 2.63 µm
increases
among particles by inreducing
the column Eddy
Bis term
Narrow particle distribution (core shell silica D /D =1.15)
ion of aDiffusion
solute isof a solute B term D /D =1.15
D90/D10=1.59 D : 2.82 µm 90 90 10
90 10
20
20 Diffusion
reduces
Column:
Column: SunShell and
SunShell
C18, 2.6(multi-path
C18, efficiency
2.650
µm µm 50mm
x 2.1 diffusion)
x 2.1 mm
ed by the Diffusion of a solute
blocked by the is Totally porous silica
Totally porous silica A solute diffuses in a pore as well as outside of particles.
A solute diffuses in a pore as well as outside of particles. Mobileas
Mobile
Totally
increases
phase:the
porous
Totally 2 µm250
porous
Abyterm
x 2.1
µm 50mm
in
reducing x 2.1 mm
Acetonitrile/water=(60/40)
phase:
VanEddy
Acetonitrile/water=(60/40)
Deemter
nce of aexistence
core,
blocked soofby a the
core,
Comparison so
existence
of Particle Size Distribution Packing state of core shell and fully porous silica
16
16 Diffusion
Sample
Equation
Sample: Naphthalene(multi-path
: Naphthalene . diffusion)
that a
solute diffuses solute diffuses
of a core, so that a solute as the A term
25 degree in Van
C Core shell 2.6 µm Deemter

(μm)
12 25 degree C Core shell 2.6 µm

(μm)
less in a core shell silica
Comparison of Particle Size Distribution Packing state of core shell and fully porous silica 12 Equation
40 degree.C Core shell 2.6 µm
n a corecolumn
shell silica
diffuses less in a core 40 degree C Totally porous 2 µm 40 degree C Core shell 2.6 µm

height
than in a totally 8 40 degree C Totally porous 2 µm
B term

height
mn than porous
in a totally
shell silica column
Diffusion
silica column. of a than
solute is 8 20
Core shell silica A core without pores blocks diffusion of a solute. Column: SunShell C18, 2.6 µm 50 x 2.1 mm
s silica Consequently
column. blocked
in a totally by the
porous silica

Plate
Totally porous 2 µm 50 x 2.1 mm
B term in of shell
Core
Diffusion silica
a solute is B term
Totally
A porous
core silica
without A solute
pores blocksdiffuses in a pore
diffusion of a as 4
well as outside of particles.
solute. 20 Mobile phase: Acetonitrile/water=(60/40)

Plate
equentlyVan Bcolumn.
term existence
Deemter of a core,
inConsequently
Equation
blocked by theso 4 16 Column: SunShell C18, 2.6 µm 50 x 2.1 mm
Sample
Totally: Naphthalene
porous 2 µm 50 x 2.1 mm
Totally porous silica A solute diffuses in a pore as well as outside of particles.
0
Deemterreduces
Equation
B termin that
inthe acore
Van solute
Deemter diffuses
existence of a core, so 16
Mobile phase: Acetonitrile/water=(60/40)
25 degree C Core shell 2.6 µm

Plate height (μm)

0 0,2 12 0,4Sample0,6
: Naphthalene
0,8 1
shell silicaless in athat
column. core shell
the silica
ainsolute diffuses 0 40 degree C Core shell 2.6 µm
es in the core
Equation reduces Flow rate 25 degree C Core shell 2.6 µm
(mL/min)

Plate height (μm)

0 12
0,2 0,4 40 0,6 40 degree0,8C Totally 1
porous 2 µm
column lessthanininaacoretotally
shell silica 8
degree C Core shell 2.6 µm
core shell
silica column. porous silica silica column. Difference
Difference in
of longitudinal
longitudinal diffusion
diffusion Plot of Flow rate and 40 Plates
degree C Totallyheight
porous 2 µm
column than in a totally
column. 8 Flow rate (mL/min)
Core shell silica A core without pores blocks diffusion of a solute.
porous B
Consequently silica
termcolumn.
in Difference of Core shell silica diffusion A core without pores blocks diffusion of a solute. Plot PlotofofFlowPlate
4 height
rate and vs Flowheight
Plates rate
longitudinal
Consequently B term Asinshown in the left figure, a core shell
4
C term Van Deemter Equation
Van Deemter Equation
reduces in the core particle has a core so that the diffusion path 0
0
0 0,2 0,4 0,6 0,8 1
reduces in the core
erm As shown of in the leftshortens
figure, and a core shell Diffusion 0 0,2 0,4 Diffusion
0,6 0,8 1
shell silica column. samples mass transfer Sample Flow rate (mL/min)
shell silica column.
particle has
in Van
a core
becomes soThis
fast.
Deemter
that the diffusion
means
Difference
EquationDifference
that the C
of longitudinalpathterm
of longitudinal
reduces. In
C TERM
diffusion
diffusion
other
Ca. 2.0 µm

Diffusion
emanates in a
Plot
particlePlot
after of Flow
it Flow
of
2.0
Flow rate (mL/min)
raterate
µm
and
andDiffusion
Plates Plates
heightheight
of samples shortens and mass transfer sample enters.
Sample sample
Ca. 2.0 µm 2.0 µm
becomeswords, HETPmeans
fast. This (theoretical
inthat
plate)
theleftCisterm kept emanates in a
C term C term even if flow
in Van Deemter Equation
As rate
shown
As increases.
particle
shown the
hasreduces.
particle a in
has core
left
in the
athe
A figure,
so
core In
that
so
µmacore
2.6figure,
other
thatthe
core
a coreshell
shell
thediffusion pathsample
path
particle after it
sample
shell particle As shows
shown as same column
left figure, adiffusion
core shell enters.
Diffusion Diffusion
words, HETP (theoretical
of of samples
samples
efficiency asparticle
a totallyhas plate)
porousa
is
shortens
shortens core
kept
and
sub-2
so
and masstransfer
mass transfer
µm the diffusion
that Ca.
Diffusion
2.0 µm
Sample
Sample 2.0 µm
Diffusion
becomes fast. This means that the C term Ca. 2.0 µm emanates in a 2.0 µm
even if flow rate
particle. increases.
becomes
path
fast.A
of samples 2.6
This µmmeans core that
shortensreduces.
the
and mass
C term C o r e S h e ll
emanates in a
particle after it
particle afterT ito ta lly
in Vanin Van
DeemterDeemter Equation
Equation reduces. In other
In other sample enters. sample
shell particle shows
The right figureas
words,
transfer same
shows HETP
becomescolumn
that a diffusion
(theoretical
fast.plate) width
plate)
This is keptthat
means sample enters. p o r ou s sample
efficiency as a totally
of a words,
sample in a HETP
porous
2.6 µm (theoretical
sub-2
core shell
µm particle is kept
theeven if flowin rate increases. AEquation
2.6 µm core
even ifCflow
term Van
rate shows Deemter
increases. A 2.6 µm core re-
particle. and a 2 µm totally shell
duces.
porous
particle
In other
particle.
words,
Both
as same
HETP
column
(theoretical
C o r e S h e ll 2.0T o µm
ta lly
diffusion shellefficiency
widths particle
are shows
almost as same
same.
as a totally The
porous column
2.6
sub-2 µm
The right figure shows that a diffusion width 1.6 µm p o r ou s
µm core efficiency
plate)
shellparticle. is
particleas aistotally
kept even porous sub-2 µm
if
superficially flow rate increas- C o r e S h e ll T o ta lly
of a sample in a 2.6 µm
particle.
es.The A core
2.6
right μm shell
figurecore particle
shellthat
shows particle shows
a diffusion width 2.6 µmC o r e S h e ll p o r ou
T so ta lly
porous, so that the diffusion width becomes
and a 2 µm totally
The porous
theright
of a
same particle.
figure
sample columnshows
in
narrower than particle size. Same diffusion a Both
2.6 that
µm a
core
efficiency diffusion
shell
as a width
particle
totally
Diffusion of sample in core shell and totally porous p o r ou s
silica
Comparison of diffusion path and a 2 µm totally porous particle. Both
2.0 µm
diffusionmeanswidthssame are almost
of aporous
sample
efficiency. same.
in
Sub-2 a 2.6
μm The
µm 2.6
core
particle. shell particle 2.0 µm
and a diffusion
2isµm widths porous
totally are almost same.Both
particle. The 2.6 1.6 µm 1.6 µm
µm core shell particle µm core
superficially
shell particle is superficially 2.0 µm
porous, so that the diffusion
diffusion widths widthare almost
becomes same. The 2.6 2.6 µm 1.6 µm
The rightso
porous, figure
that theshows the diffusion
diffusion width becomes 2.6 µm
µm core shell particle is superficially
Comparison of Performance narrower than particle by Plate/Pressure
size.
narrower
width Same
a than
of that sample diffusion
particle
in asize.
2.6widthSame
μm Diffusion
core shell of Diffusion
diffusion sampleofinsample core shell
in coreand shell totally
and totally porous
poroussilica
silica
arison of diffusion path Comparison of diffusion path porous, means so sameathe diffusion
efficiency. becomes 2.6 µm of a sample in core shell
Diffusion
means same efficiency. particle
narrower and
than particle 2 μm totally
size. press.porous
Same diffusion par- Diffusion of sample and totallyin coreporousshellsilica
and totally porous silica
Comparison of diffusion path Plate Back press. (MPa) Plate/back
Sunniest C18 –HT 2.0 µm 9,900 ticle.
means Both diffusion
16.7same efficiency.widths 593 are almost Under a constant back pressure
Brand A C18 1.9 µm
Comparison 7,660
of Performance the 16.3same. byThe 2.6 μm 470
Plate/Pressure core shell particle condition, SunShell C18 showed
Brand B C18 1.8 µm 10,100 19.6 515
mparison ofC C18
Brand Performance
1.7 µm by Plate/Pressure
11,140
is superficially
32.0
porous, so that the dif- more than 2 times higher
348
SunShellComparison
C18 2.6 µm of Performance
9,600 Plate
fusion
9.7 by Plate/Pressure
Back press. (MPa)990
width becomes Plate/back press. performance to compare with
narrower than
Sunniest C18 –HT 2.0 µm 9,900 16.7 593 Under a constant back pressure
Plate particle
Back press. (MPa) size. Same diffusion
Plate/back press. means totally sub-2µm porous C18s.
Brand
Sunniest C18 –HT 2.0Brand
A C18 1.9 µm
µm B C18 1.8 µm
7,660 16.3 470 condition, SunShell C18 showed
nniest C18 –HT 2.0 Aµm 9,900 Plate10,100 Back press.19.6
16.7 (MPa)
593 515press.Under a constant back pressure
Plate/back
same efficiency. more than 2 times higher
Brand C18 1.9 µmC18
Sunniest Brand–HT
C C182.01.7
µmµm 9,90011,140 16.732.0 348
593 Under a constant back pressure
and A C18 1.9Brand 7,660C18 2.6 µm
µm B C18 1.8 µm SunShell
Brand A C18 1.9 µm
16.3 9,600
7,660 16.3 9.7 470 990
470 condition, SunShellSunShell
performance
condition, C18
to showed
compare with
C18 showed
and B C18 1.8Brand
µm C C18 10,100 19.6 515
1.7 µm
Brand B C18 1.8 µm
Sunniest C18 –HT 2.0 µm
10,100 19.6 515 more thantotally
2 sub-2µm
times porous C18s.
higher
more than 2 times higher 5
and C C18 1.7SunShell
µm Brand
C18 11,140
C C18
2.6 µm 1.7 µm 32.0
11,140 32.0 348 348
nShell C18 2.6 µm Brand A C18
SunShell C189,600
2.6 µm 1.9 µm 9.7
9,600 9.7 990 990 performance to compare
performance with with
to compare
0 µm
Brand B C18 1.8 5 000 10 000 0 10 20 30 0 250 500 750 1000
 SUNSHELL

C18 - 2.6 µm
HIGHEST RETENTION / LARGEST STERIC SELECTIVITY / LOWEST BACKPRESSURE

SunShell C18, 2.6μm

Retention of standard samples and back pressure were compared for five kinds of core shell type
C18s. Company A C18 showed only a half retention in comparison with SunShell C18. Steric
Characteristics of SunShell
selectivity becomes C18
large when ligand density on the surface is high. SunShell C18 has the largest
steric selectivity as well as the highest ligand density leading
Core shell silica toL1)
C18 (USP the longest retention time.
Particle Pore Specific Carbon Bonded Maximum operating Available pH
End-capping
size diameter surface area content phase pressure range

SunShell C18 2.6 µm 9 nm 150 m2/g 7% C18 Sunniest End-capping 60 MPa or 8,570 psi 1.5 - 10
SUNSHELL C18 COMPARISON
Comparison of standard samples between core shell C18s
Column:
k6=5.4 7
5 Company A C18 Company A C18, 2.6 μm 150 x 4.6 mm (26.1 MPa)
2
1
3 4 6 26.1 MPa Company B C18, 2.6 μm 150 x 4.6 mm (22.7 MPa)
Mobile phase:
Company CH3OH/H2O=75/25
C C18, 2.7 μm 150 x 4.6 mm (30.6 MPa)
1 2 Flow rate: 1.0
Company mL/min,
D C18, 2.7 μm Temperature: 40°ºC
150 x 4.6 mm (22.2 MPa)
5
k6=7.4
7 Company B C18
SunShell
Sample: 1 =C18, 2.6 μm
Uracil, 2 =150 x 4.6 mm3(21.8
Caffeine, MPa)
= Phenol,
3 6 22.7 MPa
4
4 Mobile phase: CH3OH/H
= Butylbenzene, O=75/25
5 = 2o-Terphenyl,
1 2 7 Flow rate: 1.0 mL/min, Temperature: 40 ºC
5 Company C C18 6 = Amylbenzene, 7 = Triphenylene
3
k6=9.0 Sample: 1 = Uracil, 2 = Caffeine, 3 = Phenol, 4 = Butylbenzene
4 6 30.6 MPa 5 = o-Terphenyl, 6 = Amylbenzene, 7 = Triphenylene
5 7 Hydrogen bonding Hydrophobicity Steric selectivity
23 k6=9.7 Company D C18
1
4 6
22.2 MPa Hydrogen bonding Hydrophobicity Steric selectivity
Company A C18 (Caffeine/Phenol)
0.48 1.54
(Amylbenzene/Butylbenzene) 1.20
(Triphenylene/o-Terphenyl)

Company B C18 0.480.35 1.56

1.54 1.50
1.20
2 5 7 Company A C18
3 SunShell C18
6 k6=10.4 Company B C18
Company C C18 0.350.42 1.56
1.57 1.50
1.25
1 21.8 MPa 4
Company C C18
Company D C18 0.420.44 1.57
1.60 1.25
1.31
Company D C18 0.440.39 1.60 1.31
0 2 4 6 8 10 12 14 16 18 20 22 24 Sunshell C18 1.60 1.46
SunShell C18 0.39 1.60 1.46
Retention time/min
Retention of standard samples and back pressure were compared for five kinds of core shell type C18s. Company A C18 showed
only a half retention to compare with SunShell C18. Steric selectivity becomes large when ligand density on the surface is high.
6
SunShell C18 has the largest steric selectivity so that it has the highest ligand density. This leads the longest retention time.
 Mobile phase: Acetonitrile/20mM phosphate buffer pH7.0=(60:40) Mobile phase: Acetonitrile/10mM acetate ammonium pH6.8=(40:60)
16000 14000

14000 BEST PEAK SHAPE AVAILABLE 12000

12000
10000 Sunshell C18

Theoretical plate
Theoretical plate

100 倍 Company A C18

10000 SunShell C18
30倍
Company C C18 8000 Company P C18
8000 Sunniest C18 3um Company T C18
Company A C18 6000 Company S C18
6000
Amitriptyline overloads
more at
Company B C18much compared as refers to loading capacity better sensitivity and accuracy of the
Company D C18 4000
acetonitrile/buffer
4000 mobile phase than of amitriptyline. Thanks to the unique method.
methanol/buffer which causes tail- bonding technology Sunshell gives ex-
2000
ing. 2000
Five kinds of core shell C18s were traordinary peak shape, which means
0 0
0,001 0,01 0,1 1 10 0,001 0,01 0,1 1 10
Sample weight/μg Sample weight/μg
3 4
TF=1.18 4
1 2 SunShell C18 Sample: TF=1.20
(core shell) 1
2
3 SunShell C18
1 = Uracil (0.07µg) (core shell)
TF=1.42 C C18 2 = Propranolol (1.53µg)
(core shell) 3 = Nortriptyline (0.32µg) TF=1.89 C C18
4 = Amitriptyline (0.32µg) (core shell)
TF=1.25 Sunniest
C18 3 μm (fully porous)
TF=2.61 A C18
A C18 (core shell)
TF=2.43 (core shell)
TF=2.73 B C18
TF=3.21 B C18 (core shell)
(core shell)
TF=3.24 D C18
TF=4.38 D C18 (core shell)
(core shell)
0 1 2 3 4 5 6 7 8 9 10 0 1 2 3 4 5 6 7 8 9 10
Retention time/min Retention time/min

Theoretical plate was calculated by 5σ method 4.4%

Mobile Phase: Mobile Phase:
using peak width at 4.4% of peak height.
• Acetonitrile/20 mM phosphate buffer pH 7.0 (60/40) • Acetonitrile/10 mM ammonium acetate pH 6.8 (40/60)

Mobile phase: Acetonitrile/0.1% formic acid=(30:70)

4,5 USP Tailing factor
Comparison column
3.6
4 1. Kinetex C18, 2.6 μm
Company D C18 2. Accucore C18, 2.6 μm
2.9
Company Company
A C18: B C18
Kinetex C18
USP tailing factor

3,5 3. PoroShell C18 EC, 2.7 μm

Company
Company B C18: A C18 C18
Accucore 2.4 4. Ascentis Express C18, 2.7 μm
Company
3 Company
C C18: C C18 C18 EC
PoroShell 5. SunShell C18, 2.6 μm
SunShell C18 1.8
Company D C18: Ascentis Express C18
2,5
1.3
7倍
2

1,5
Amitriptyline overloads at low weight 7
1
when acetonitrile/0.1% formic acid
 EXPANDED pH RANGE DUE TO
THE SUNSHELL BONDING TECHNOLOGY
◆Evaluation of Stability
SUNSHELL C18 STABILITY
Accelerated acidic test
100
◆Evaluation of Stability

80 Alkaline test
Accelerated acidic test
Relative retention/%

100
100
60

plate of butylbenzene/%
SunShell C18
80 Company D C18 80 Alkaline test
40 SunShell C18
Relative retention/%

Company B C18
60 Company A C18
1% TFA 100
60 Company D C18

of butylbenzene/%
SunShell C18
20 Compnay
80 ºC Company A C18
CompanyCDC18
C18 80
40 40 Company B C18
SunShell C18
Company B C18
0 1% TFA 60 Company
CompanyCDC18
C18 pH10
0 20 Company
40 A C1860 80 100 120
80 ºC 20
50 ºC
Relative
20 Compnay C C18 Company A C18
Time/h
40 Company B C18
Relative plate 0
Durable
0 test condition 0 1 000 2 000 Company
3 000 C4C18
000 5pH10
000 6 000
0 20 40 60 80 100 120 20
Elution volume/mL 50 ºC
Column size: 50 x 2.1 mm Time/h
Mobile phase: CH3CN/1.0% TFA, pH1=10/90 0
Durable test condition Durable0test condition
1 000 2 000 3 000 4 000 5 000 6 000
Flow rate: 0.4 mL/min
Temperature: 80 ºC Elution volume/mL
Column size: 50 x 2.1 mm Column Size: 50 x 2.1 mm
Mobile phase: CH3CN/1.0% TFA, pH1=10/90 Mobile phase:
Measurement
Flow rate: 0.4 condition
mL/min Durable test condition
CH3OH/20mM Sodium borate/10mM NaOH=30/21/49 (pH10)
Temperature: 80 ºC Flow rate: 0.4 mL/min
Column size: 50 x 2.1 mm Column Size: 50 x 2.1 mm
Temperature: 50 ºC
Mobile phase: CH3CN/H2O=60/40 Mobile phase:
Measurement condition CH3OH/20mM Sodium borate/10mM NaOH=30/21/49 (pH10)
Flow rate: 0.4 mL/min
Stability under acidic Measurement
Stability under condition
0.4basic pH condition was evaluated at 50°C
Temperature: 40 ºCpH condition was evaluated at 80°C Flow rate: mL/min
using Column size: 50 x 2.1 mm using methanol/Sodium borate buffer pH 10 (30:70) as
Sample: 1 = Uracil trifluoroacetic
acetonitrile/1% (t0) acid solution (10:90) as Temperature: 50 ºC
Mobile
mobile phase. phase: CH
100% CN/H
aqueous
3 2O=60/40
mobile phase expels from the Column
mobile Size: 50
phase. x 2.1 mm
Sodium borate is used as an alkaline stan-
2 = Butylbenzene
poresFlow rate:packing
of C18 0.4 mL/minmaterials by capillarity and packing Mobile
dard phase:for
solution
Measurement
CHpH3CN/H 2O=60/40
meters,
condition which allows for a high buffer
Temperature:
materials 40 ºC
do not deteriorate. Adding 10% acetonitrile to the Flow rate:
capacity. 0.4 mL/min
Elevated temperature of 10°C reduces column life
mobileSample:
phase 1 = Uracil
enables 0)
(taccurate evaluation. toTemperature:
one third. 40 other
The ºC company shows stability when tested
Stability under acidic pH condition was evaluated at 80 ºC using Column Size: 50 x 2.1 mm
2 = Butylbenzene atSample: 1 (room)
= Butylbenzene
acetonitrile/1% trifluoroacetic acid solution (10:90) as mobile Mobile phase: CH3CN/H2O=60/40If room temperature is 25°C,
ambient temperature.
phase. 100% aqueous mobile phase expels from the pore of column life is
Flow rate: 0.4sixteen
mL/min times longer than at 50°C.
packing materials by capillarity and packing materials doesn’t Temperature: 40 ºC
8
Stability under
deteriorate. 10%acidic pH condition
acetonitrile was evaluated
in a mobile at 80
phase allows anºC using Stability under basic pH condition was evaluated at 50 ºC using
Sample: 1 = Butylbenzene
methanol/Sodium borate buffer pH 10 (30:70) as mobile phase.
acetonitrile/1%
accurate trifluoroacetic
evaluation. 1-3) acid solution (10:90) as mobile
 BLEEDING TEST USING LC/MS

The high stability of the SunShell columns also means low bleeding
in LC/MS analysis as shown here.

Column size: 50 x 2.1 mm

Mobile phase:
A) 0.1% Acetic acid
TIC of Q1 B) CH3CN
Gradient:
Time: 0min 1min 5min 7min
%B: 5% 5% 100% 100%
Flow rate: 0.4 mL/min
Temperature: 40 °C
MS: ABI API-4000 Ionization:
Turboionspray (cation)
Measurement mode:
Q1 Scan m/z 100-1000

9
 SUNSHELL C18 EFFICIENCY
Efficiency of SunShell C18

Column: SunShell C18, 2.6 μm 50 x 2.1 mm

3 Column: SunShell C18, 2.6 μm 50 x 2.1 mm
Plates (3)=10,300
Plate(3)=10,300 Mobile phase: CH3CN/H2O=60/40
Flow rate: 0.3 mL/min
2
1 4 Pressure: 7 MPa
Temperature: 23 ºC
UHPLC: Jasco X-LC

Sample: 1 = Uracil
0 1 2 3 4 5
Retention time/min 2 = Toluene
3 = Acenaphthene
4 = Butylbenzene
Column: SunShell C18, 2.6 μm 150 x 4.6 mm
1 Column: SunShell C18, 2.6 μm 150 x 4.6 mm
Mobile phase: CH3CN/H2O=70/30
Plates (3)=38,000
Plate(3)=38,000 Flow rate: 1.0 mL/min
Pressure: 15.5MPa
2 Temperature: 25 ºC
3 4 UHPLC: Jasco X-LC

0 2 4 6 8 10 Efficiency=253,000 plate/m
EFFICIENCY = 253,000 plates/m
Retention time/min

Examples of transfer from a conventional 5 µm column to SunShell column

Inner diameter
Isocratic separation
ORDERING INFO OF SUNSHELL
(mm)
1.0 2.1 3.0 4.6 USP category

HPLC 1
Brand F C18, 5 µm 250
Length
x 4.6(mm)
mm Catalog no
N(4)=19,313
Catalog no Catalog no Catalog no Catalog no
3
30 ---5 Column:
CB6931 CB6331 CB6431
2 4
50 CB6141 Brand F C18,CB6341
CB6941 5 μm 250 x 4.6CB6441
mm
75 --- SunShell C18,
CB6951 2.6 μm 100 x 4.6
CB6351 mm
CB6451
Sunshell C18, 2.6 µm L1
100 CB6161 Mobile phase:
CB6961 CB6361 CB6461
3 SunShell C18, 2.6 µm 100 x 4.6 mm 150 CB6171 CH3CN/20mM
CB6971 Phosphoric acid
CB6371 = 45/55
CB6471
1 5
2 4 250 ---
N(4)=20,287 ---
Flow rate: 1.0CB6381
mL/min, CB6481
1/3 of analysis time
1.8 mL/min at the lowest chromatogram
Temperature: 25 ºC
Pressure: 9.5 MPa for Brand F C18 5 μm
10 0 2 4 6 8 10 12 14 16 18 20 22 24 13.4 MPa for SunShell C18 2.6 μm
Retention time/min
Detection: UV@230 nm
3 N(4)=20,287
 SUNSHELL
SunShell C18, 5 μm
SunShell C18, 5 μm
Characteristics of SunShell C18, 5 μm
Characteristics of SunShell C18, 5 μm
C18 - 5 µm
Core shell silica C18 (USP L1)
Particle Pore Specific Carbon Bonded Maximum operating Available pH
Core shell silica C18 (USP L1)
End-capping
size diameter surface area content phase pressure range
Particle Pore Specific Carbon Bonded Maximum operating Available pH
4.6 µmsurface
9 nm 90 m2/g 5.5 % End-capping
C18 Sunniest End-capping 1.5 - 10
SunShell
size C18
diameter area content phase pressure 60 MPa or 8,570
range psi

SunShell C18 4.6 µm 9 nm 90 m2/g 5.5 % C18 Sunniest End-capping 60 MPa or 8,570 psi 1.5 - 10
Comparison Can
of retention
be used inand
anyplate using
L1 method HPLC
- but with improved performance.
Comparison
1 of retention and plate using HPLC
2 SunShell C18 5 µm N6=20,000 Column size: 150 x 4.6 mm
1 Column size: 150Mobile x 4.6phase:
mmCH OH/H O=75/25
8 MPa 7
2 SunShell C18 5 µm
3 2
N
5 6=20,000 Column size: 150 x 4.6 mm
Mobile phase: CH3OH/H2O=75/25
Flow rate: 1.0 mL/min
8 MPa 3 4 7 6 Mobile phase: CH3OH/H2O=75/25
Temperature: 40 ºC
3 5 Flow Flow
rate:rate:
1.01.0
mL/min
mL/min
4 6 Sample: 1 = Uracil
N6=31,000 Temperature:
Temperature:40°ºC
40 ºC
2 = Caffeine
SunShell C18 2.6 µm 7 Sample: 1 = Uracil
2 N =31,000
5 Sample: 1 = Uracil 3 = Phenol
SunShell
1 C18 212.6MPa
µm 4 6
76
2 = Caffeine
4 = Butylbenzene
2 3
5
2 = Caffeine
3 = Phenol
1 21 MPa 4 6
5 = o-Terphenyl
3 3 = Phenol
4 = Butylbenzene
6 = Amylbenzene
5 = o-Terphenyl
4 = Butylbenzene 7 = Triphenylene
6 MPa N6=14,000 6 = Amylbenzene
HPLC: Hitachi LaChrom ELITE
5 = o-Terphenyl
7 = Triphenylene
6 MPa N 6 =14,000 (Tubing, 0.25 mm i.d.)
HPLC:
6 =Hitachi LaChrom ELITE
Amylbenzene
(Tubing, 0.25 mm i.d.)
7 = Triphenylene
Totally porous silica Core shell silica HPLC: Hitachi
Core shellLaChrom
silica ELITE (Tubing, 0.25 mm i.d.)
Sunniest C18, 5 μm SunShell C18, 2.6 μm SunShell C18, 5 μm
Totally porous silica Core shell silica Core shell silica There is a little
Specific surface area Sunniest
Totally porous
C18, 5silica
2/g
μm 340 mSunShell C18,Core 150
shell
2.6 μm m2/g
silica SunShell C18, 5 μm 90shell
Core m2/gsilica
Sunniest C18,Retention
5 µm SunShell C18, 2.6 µm SunShell C18, 5 There
µm is a difference of k between
little
Retention
150 m2/gRetention Retention 90 m2/gRetention Retention
Specific surface area 340 m2/g
difference of totally porous and core
k between
2 time (tR) factor (k) time (tR) factor (k) time (t ) factor (k)
Specific surface area 340 m /g 150 m /g
2 R 90 m /g
2
Retention Retention Retention Retention Retention Retention shell
There particles.
is a small difference
1) Uracil
time (tR) factor1.70
(k) 0
time (tR) 1.34
factor (k) time0(tR) 1.30(k)
factor 0totally porous and core
Retention Retention Retention Retention Retention Retention of k between totally porous
6) Amylbenzene time1.70 19.96 10.74 time (tR) 16.56 11.36 13.43 shell
9.33 (k) particles.
1) Uracil (tR) factor
0 (k) 1.34 0 factor (k)
1.30 time
0 (tR) factor
and core shell particles.
6) AmylbenzeneRelative value of Amylbenzene
1) Uracil 19.96
1.70
100%
10.74
0
100%
16.56 83%
1.34 11.36
106%
13.43
0
67%
9.33
1.30
87%
0

6)Relative value of Amylbenzene

Amylbenzene 100%
19.96 100%
10.74 83% 16.56 106% 67%
11.36 87%
13.43 9.33

Comparison between normal and semi-micro HPLC

Relative value of Amylbenzene 100% 100% 83% 106% 67% 87%

Comparison between normal and semi-micro35,150

HPLC 34,462
Inner diameter 13,993
ORDERING INFO OF SUNSHELL 42,053 1.0 2.1
30,564 40,3203.0 4.6 USP category
35,150
(mm) 34,462
13,993 40,320 41,043 34,052
42,053 Length (mm) 30,564
Catalog no40,139 Catalog no Catalog no Catalog no Catalog no
Flow cell Response Sampling Tubing ID 41,043 34,05237,854
Normal Sampling
0.1 secTubing
0.4 ID
sec 0.25 mm 40,139 41,610
Flow cell Response
150 --- --- 37,854 39,255CB3471
CB3371
Normal Semi-micro
0.1 Sunshell
sec 0.1 sec50.25
C18,
0.4 sec µm0.4
mmsec 0.13 mm 41,610 L1
250 --- --- 39,255
CB3381 CB3481
Semi-micro 0.1 sec 0.40.05
Semi-micro sec sec0.130.05
mmsec 0.13 mm
Semi-micro 0.05 sec 0.05 sec 0.13 mm
0 1 2 3 4 5 6 7 8 9 10 11 11
Retention time/min
0 1 2 3 4 5 6 7 8 9 10 11
 SUNSHELL
SunShell C18-WP, RP-AQUA, C8, Phenyl, PFP, 2.6 μm
SunShell C18-WP, RP-AQUA, C8, Phenyl, PFP, 2.6 μm (Pentafluoropheny)
(Pentafluoropheny)
◆ Characteristics of SunShell
◆ Characteristics ofCore
SunShell
shell silica
ULTIMATE SELECTIVITY
size
Particle Pore
diameter
Specific
surface
Specific
surface
area Carbon
Core shellPore
Particle
Carbon
content
silica
Bonded phase
Bonded phase
FOR YOUR ANALYSIS
USP L line
USP L line End-capping
End-capping
Maximum
Maximum
operating
pressure
operating
Available
pH range
Available
SunShell C18size 2.6diameter
μm 9nm content 7%
area 150 m2/g C18 L1 Sunniest endcapping pH range
60 MPa
pressure 1.5 - 10
SunShell 2.6 μm2.6 μm
C18 C18-WP 9nm 16 150 90 m2/g
nm m2/g 5% C18 C18 L1 Sunniest Sunniest endcapping
60 MPa60 MPa
1.5 - 101.5 - 10
SunShell
SunShell
SunShell C18-WP 2.6 μm2.6 μm
RP-AQUA 16 nm 16 nm
90 m2/g
7%
90 m2/g
5%C18 -WP / RP-AQUA
4% / C8
C18 C28 / PHENYL
L1
Equivalent
L1 /endcapping
toSunniest PFP
L62 Sunniest - 2.6
endcapping
endcapping µm
60 MPa60 MPa
1.5 - 10 2 - 8 a)

SunShell C82.6 μm2.6 μm

SunShell RP-AQUA 16 nm 9nm
90 m2/g /g
150 m24% 4.5% C28 C8 L7 Sunniest
Equivalent to L62 Sunniest endcapping
endcapping 60 MPa60 MPa2 - 8a) 1.5 - 9
SunShell
SunShell C8 Phenyl2.6 μm2.6 μm
9nm 9nm
150 m2150 2/g
/g m4.5% 5% C8Phenylhexyl L7 L11 Sunniest
Sunniest endcapping
endcapping 60 MPa60 MPa1.5 - 9 1.5 - 9
Phenyl PFP2.6 μm2.6 μm
SunShellSunShell 9nm 9nm
150 m2150 /g
/g m25% 4.5% Phenylhexyl
Pentafluorophenyl L11 L43 Sunniest TMS endcapping60 MPa60 MPa
endcapping 1.5 - 9 2 - 8
SunShell PFP 2.6 μm 9nm 150 m /g
2 4.5% Pentafluorophenyl L43 TMS endcapping a)60 MPa
Under 2 - 8 condition
100% aqueous
◆ Comparison of standard samples Column: SunShell C18, C18-WP,a) RP-AQUA, C8, Phenyl, PFP, 2.6
Under 100% aqueous condition
◆ Comparison
1 2,3
5
of
7
standard samples μm
Column: SunShell C18, C18-WP, RP-AQUA, C8, Phenyl, PFP, 2.6 μm
SunShell PFP 150 x 4.6 mm
1 2,3
5 4 6 7 Column: Mobile
SunShell150 x 4.6
C18, mm RP-AQUA, C8, Phenyl, PFP, 2.6 μm
C18-WP,
SunShell PFP phase: CH3OH/H2O=75/25
Mobile phase:
150 x 4.6 mm CH OH/H 2O=75/25
4 6 3
2,3 Flow
Flow rate:
rate: 1.0 1.0
mL/min
Mobile phase: CH3OH/H2O=75/25
mL/min
1 5,7
2,3 SunShell Phenyl Flow rate: 1.0 mL/min40 ºC40°ºC
Temperature:
Temperature:
1 4 6
5,7 Sample:
Temperature: 1 =ºCUracil
Sample:
40 1 = Uracil
SunShell Phenyl 2 = Caffeine
4 6 Sample: 12==Uracil
Caffeine
1
3 = Phenol
23==Caffeine
2 7 Phenol
4 = Butylbenzene
5 SunShell C8 3 = Phenol
1
2 74 6 4 = Butylbenzene
5 = o-Terphenyl
4 = Butylbenzene
3 5 SunShell C8 6 = Amylbenzene
4 6 55==o-Terphenyl
o-Terphenyl
3 1 7 7 = Triphenylene
66==Amylbenzene
Amylbenzene
2 5
SunShell RP-AQUA 77==Triphenylene
1 7 6 Triphenylene Hydrogen bonding Hydrophobicity Steric selectivity
2 3 54
SunShell RP-AQUA (Caffeine/Phenol) (Amylbenzene/Butylbenzene) (Triphenylene/o-Terphenyl)
4 6 Hydrogen bonding Hydrophobicity Steric selectivity
3 1 PFP 1.00 1.31 2.38
2 7 (Caffeine/Phenol) (Amylbenzene/Butylbenzene) (Triphenylene/o-Terphenyl)
Hydrogen bonding Hydrophobicity Steric selectivity
5 PFP Phenyl 1.00 1.00 1.31 1.48 2.38 1.01
1
2 4 7 6 SunShell C18-WP
3 PFP 1.00 1.31 2.38
5
4 6 SunShell C18-WP 7 PhenylC8 1.00 0.32 1.48 1.46 1.01 1.08
3 1 5 Phenyl 1.00 1.48 1.01
2
7
1 SunShell C18 4 6 C8 RP-AQUA
C8 0.32 0.52
0.32 1.46 1.46
1.52 1.08
1.08 1.30
2 5
3
SunShell C18 4 6 RP-AQUA RP-AQUA 0.52 0.40
0.52 1.52 1.52 1.30
1.30
C18-WP 1.55 1.35
3
C18-WP 0.40
C18-WP
SunShell C18 0.40 0.39 1.55 1.55
1.60
1.35
1.35 1.46
0 2 4 6 8 10 12 14 16 18 20 22 24 Sunshell C18 0.39 1.60 1.46
0 2 4 6 8 10 12Retention
14 16time/min
18 20 22 24 SunShell C18 0.39 1.60 1.46
Retention time/min
12
Separation of peptides Separation of amitriptyline using C8 Separation of basic compounds
Separation of peptides Separation of amitriptyline using C8
 C18 -WP / RP-AQUA / C8 / PHENYL / PFP - 2.6 µm

Inner diameter
ORDERING INFO OF SUNSHELL 1.0 2.1 3.0 4.6 USP category
(mm)
Length (mm) Catalog no Catalog no Catalog no Catalog no Catalog no
30 --- CC6931 CC6331 CC6431
50 --- CC6941 CC6341 CC6441
Sunshell C8, 2.6 µm 75 --- CC6951 CC6351 CC6451 L7
100 --- CC6961 CC6361 CC6461
150 --- CC6971 CC6371 CC6471

30 --- CF6931 CF6331 CF6431

50 --- CF6941 CF6341 CF6441
Sunshell PFP, 2.6 µm 75 --- CF6951 CF6351 CF6451 L43
100 --- CF6961 CF6361 CF6461
150 --- CF6971 CF6371 CF6471

30 --- CW6931 CW6331 CW6431

50 --- CW6941 CW6341 CW6441
Sunshell C18-WP, 2.6 µm 75 --- CW6951 CW6351 CW6451 L1
100 --- CW6961 CW6361 CW6461
150 --- CW6971 CW6371 CW6471

30 --- CR6931 CR6331 CR6431

50 CR6141 CR6941 CR6341 CR6441
Equivalent
Sunshell RP-AQUA, 2.6 µm 75 --- CR6951 CR6351 CR6451
100 CR6161 CR6961 CR6361 to L62
CR6461
150 CR6171 CR6971 CR6371 CR6471

30 --- CP6931 CP6331 CP6431

50 --- CP6941 CP6341 CP6441
Sunshell Phenyl, 2.6 µm 75 --- CP6951 CP6351 CP6451 L11
100 --- CP6961 CP6361 CP6461
150 --- CP6971 CP6371 CP6471

13
 SUNSHELL

SunShell HFC18-16, HFC18-30, 2.6 μm

HFC18 - 16 / HFC18 -For30
Characteristics of SunShell HFC18
separation of peptides and proteins
- 2.6 µm
Core shell silica C18 (USP L1)
Particl Pore Specific Carbon Maximum operating Available
Ligand density End-capping
e size diameter surface area content pressure pH range
SunShell C18-WP 2.6 μm
16 nm 90 m /g 5% 2.5 μmol/m2 Sunniest endcapping
2 60 MPa or 8,570 psi 1.5 - 10
High speed separations of proteins and peptides.
SunShell HFC18-16 2.6 μm 16 nm 90 m2/g 2.5% 1.2 μmol/m2 Sunniest endcapping 60 MPa or 8,570 psi 1.5 – 9
What is HFC18? Hexa-Functional C18 has six functional groups.
SunShell HFC18-30 2.6 μm 30 nm 40 m2/g 1.3% 1.2 μmol/m2 Sunniest endcapping 60 MPaa or 8,570 psia 1.5 - 9
The HFC18 is much more stable under acidic conditions. a: 50MPa, 7141psi for 4.6 mm i.d. column

What is HFC18? Hexa-Functional C18 has six functional groups.

This HFC18 is much more stable under acidic condition.

Sunniest
Bonding
Technology

Hexamethydichlorotrisiloxane
+
Trimethylchlorosilane (TMS)

O Si Si
Si Si O
Si O O
O O Si
O O O O Si Si
Si Si Si Si Si O O
O Si O Si O O O
O
O O HO O Si O
Si O O Si Si Si
Si O O
O Si O O
Si Si O O
Si O O Si O O O
O O O O
O O O
(X: Cl, OCH3, OC2H5)
Schematic diagram of the state of bonding
Schematic diagram of reagent
on silica surface

0,9 Proteins/peptides are often 100 analysed Durable test condition

Desorption Dv (log d) (cc/g)

0,8
atCore
acidic
shell 30pH.
nm The wide pore SunShell 95% line Column : SunShell HFC18-16 2.6 μm, 50 x 2.1 mm
Relative retention (%)

0,7 80 Mobile phase:

0,6
phases
Core shell are
16 nmoptimized for superior life
CH3CN/0.1% formic acid, pH2.6=40/60
time at extreme conditions. 60 Flow rate: 0.4 mL/min
0,5
Temperature: 70 ºC
0,4
40
0,3 0.1% formic acid, pH2.6
0,2 70 ºC
20
0,1 Measurement condition
0 0 Mobile phase: CH3CN/H2O=60/40
1 10 100 1000 0 200 400 600 800 1000 Flow rate: 0.4 mL/min
Pore Diameter (nm) Time (h) Temperature: 40 ºC
Sample: 1 = Uracil
14 Pore distribution of core shell particle Stability under LC/MS mobile phase condition 2 = Butylbenzene
 Technology

Hexamethydichlorotrisiloxane
+
Trimethylchlorosilane (TMS)

HFC18 - 16 / HFC18 - 30 - 2.6 µm Si

O
Si
Si
O
Si
O
Si
O
O O Si
O O O O Si Si
Si Si Si Si Si O O
O Si O Si O O O
O
O O HO O Si O
Si O O Si Si Si
Si O O
O Si O O
Si Si O O
Si O O Si O O O
O O O O
O O O
(X: Cl, OCH3, OC2H5)
Schematic diagram of the state of bonding
Schematic diagram of reagent
on silica surface

0,9
100 Durable test condition
Desorption Dv (log d) (cc/g)

0,8 Column : SunShell HFC18-16 2.6 μm, 50 x 2.1 mm

Core shell 30 nm 95% line

Relative retention (%)

0,7 80 Mobile phase:
Core shell 16 nm
0,6 CH3CN/0.1% formic acid, pH2.6=40/60
60 Flow rate: 0.4 mL/min
0,5
Temperature: 70 ºC
0,4
40
0,3 0.1% formic acid, pH2.6
0,2 70 ºC
20
0,1 Measurement condition
0 0 Mobile phase: CH3CN/H2O=60/40
1 10 100 1000 0 200 400 600 800 1000 Flow rate: 0.4 mL/min
Pore Diameter (nm) Time (h) Temperature: 40 ºC
Sample: 1 = Uracil
Pore distribution of core shell particle Stability under LC/MS mobile phase condition 2 = Butylbenzene

Separation of peptides

Column: SunShell HFC18-16, 2.6 μm (16 nm) 150 x 4.6 mm,

SunShell HFC18-16 SunShell C18-WP, 2.6 μm (16 nm) 150 x 4.6 mm
Mobile phase: A) 0.1% TFA in Acetonitrile/water(10:90)
B) 0.1 % TFA in Acetonitrile
Gradient program:
Inner diameter
ORDERING INFO OF SUNSHELL 1.0 2.1 3.0 4.6 USP category
(mm) Flow rate: 1.0 mL/min
Length (mm)
Temperature:
Catalog no
25 ºC
Catalog no Catalog no Catalog no Catalog no
Detection: UV@210 nm
2 50
peaks --- Sample: Tryptic
CG6941 CG6341 C CG6441
digest of cytochrome
SunShell C18-WP
Sunshell HFC18-16, 2.6 µm 100 --- CG6961 CB6361 CG6461 L1
150 --- CG6971 CB6371 CB6471

50 --- C46941 C46341 C46441

Sunshell HFC18-30, 2.6 µm 100 --- C46961 C46361 C46461 L1
150 --- C46971 C46371 C46471

10

15
 SUNSHELL

SunShell 2-EP, 2.6 μm

HARDCORE SFC SEPARATIONS
For Supercritical fluid Chromatography

2.6 μm core shell column shows only one third of back pressure to compare with 1.7 μm fully porous
column although both show almost same efficiency. By such low back pressure, a difference of
2 -EP (ETHYLPYRIDINE) - 2.6 µm
density of supercritical fluid between an inlet and an outlet of the column is reduced. Consequently, .
2.6 μm core shell column performs a superior separation for SFC.
Characteristics of SunShell 2-EP
Core shell silica
Particle Pore Specific Carbon End- Maximum operating Available pH
Bonded phase
The 2.6 μm core size column
shell diameter
showssurface areathe content
same
efficiency. By such capping
low back pressurequently, 2.6
rangeμm core shell column per-
SunShell 2-EP 2.6 μm 9 nm 150 m2/g 2.5% 2-Ethylpyridine no 60 MPa or 8,570 psi 2 – 7.5
only one third of back pressure in com- pressure, a difference of density of su- forms a superior separation for SFC.
parison with the 1.7 μm fully porous percritical fluid between an inlet and an
column. However, both show almost outlet of the column is reduced. Conse-
Comparison between SunShell 2-EP and 1.7 μm fully porous 2-EP

Figure 1: Chromatogram of the separation for he 17-

Figure 1 component mix using the Sun Shell 2-EP 150 x 3.0 mm
column. A methanol gradient of < 2 minutes was used
Figure
on the Agilent 1260 Infinity 1: Chromatogram
SFC system. SFC of the separation
for the 17-components
conditions: flow rate: 4.0mL/min; outlet pressure 160 mix using the Sun
bar; column temperatureShell
55⁰C. Gradient
2-EP 150 program:
x 3.05.0-
mm column. A meth-
7.5% in 0.20 min, then 7.5-20% in 1.3 min and held at
20% for 0.2 min. anol gradient of < 2 minutes was used on
the Agilent 1260 Infinity SFC system. SFC
Figure 2: Chromatogram of the separation for the 17-
conditions:
component mix using Acquity flow2-EP
UPC2 Viridis rate:100
4.0mL/min; outlet
x 3.0 mm column. 16 ofpressure
the 17 components
160 bar; were
column temperature
resolved. A methanol gradient
55°C.ofGradient
< 2 minutes was
program: 5.0-7.5% in 0.20
used on the Agilent 1260 Infinity SFC system. SFC
conditions: flow rate 3.5min, then
mL/min; 7.5-20%
outlet pressurein 1.3 min and held at
160
20%70⁰C.
bar; and column temperature for 0.2 min.
Gradient
program: 5.0-12.5% in 1.0 min, 12.5% for 0.25 min,
then 12.5-20% in 0.75 min.

Figure 2

16
Figure 1 component mix using the Sun Shell 2-EP 150 x 3.0 mm
column. A methanol gradient of < 2 minutes was used
on the Agilent 1260 Infinity SFC system. SFC
conditions: flow rate: 4.0mL/min; outlet pressure 160
bar; column temperature 55⁰C. Gradient program: 5.0-
7.5% in 0.20 min, then 7.5-20% in 1.3 min and held at
2 -EP - 2.6Figure
µm 20% for 0.2 min.
2: Chromatogram of the separation for the 17-
component mix using Acquity UPC2 Viridis 2-EP 100
x 3.0 mm column. 16 of the 17 components were
resolved. A methanol gradient of < 2 minutes was
used on the Agilent 1260 Infinity SFC system. SFC
conditions: flow rate 3.5 mL/min; outlet pressure 160
bar; and column temperature 70⁰C. Gradient
program: 5.0-12.5% in 1.0 min, 12.5% for 0.25 min,
then 12.5-20% in 0.75 min.
Figure 2: Chromatogram of the separa-
Figure 2 tion for the 17-components mix using
Acquity UPC2 Viridis 2-EP 100 x 3.0 mm
column. 16 of the 17 components were
resolved. A methanol gradient of < 2
minutes was used on the Agilent 1260
Infinity SFC system. SFC conditions: flow
rate 3.5 mL/min; outlet pressure 160 bar;
and column temperature 70°C. Gradient
program: 5.0-12.5% in 1.0 min, 12.5% for
0.25 min, then 12.5-20% in 0.75 min.
Courtesy of Pfizer Inc.
Courtesy of Pfizer Inc.

Inner diameter
ORDERING INFO OF SUNSHELL 1.0 2.1 3.0 4.6 USP category
(mm) 11
Length (mm) Catalog no Catalog no Catalog no Catalog no Catalog no
30 --- CE6931 CE6331 CE6431
50 --- CE6941 CE6341 CE6441
Sunshell 2-EP, 2.6 µm 75 --- CE6951 CE6351 CE6451
100 --- CE6961 CE6361 CE6461
150 --- CE6971 CE6371 CE6471

17
 SunShell HILIC-Amide, 2.6 μm
For Hydrophilic Interaction Chromatography
Characteristics of SunShell HILIC-Amide
SUNSHELL
Core shell silica Amide (USP L68)
Particle Pore Specific Carbon Bonded Maximum operating Available pH
End-capping
size diameter surface area content phase pressure range

SunShell HILIC-Amide, 2.6 μm

SunShell HILIC-Amide 2.6 μm 9 nm 150 m2/g 3% Amide no 60 MPa or 8,570 psi 2-8

Stationary phase of HILIC-Amide HILIC -AMIDE - 2.6 µm For Hydrophilic Interaction Chromatography
Characteristics of SunShell HILIC-Amide
R: Hydrophilic group Amide (USP L68)
Core shell silica
For
Particle
Hydrophilic
Specific Pore
Carbon
Interaction
Bonded
Chromatography.
Maximum operating Available pH
End-capping
size surface area diameter
content phase pressure range
SunShell HILIC-Amide
Highly
2.6 μm efficient
9 nm
separation
150 m /g
of
3%
very polar
Amide
compounds.
no
2 Rapid
60 MPa or 8,570 psi
equilibration.
2-8

Stationary
Stationary phase
phase HILIC-Amide consists of AMIDE and The
of HILIC-Amide
of SunShell stationary GROUP,
HYDROPHILIC phase ofso SunShell
that this HILIC-Amide
stationary
consists of AMIDE and HYDROPHILIC GROUP,
phase is more polar than an individual group. High speed separation is leaded by core shell structure that derives
high efficiency and fast equilibration.
R: Hydrophilic group so that this stationary phase is more polar than
an individual group. High speed separation is a
result of core shell structure that derives high
Separation of Nucleic acid bases: Comparison of the other coreand
efficiency shell hilic
fast columns
equilibration.
Stationary phase of SunShell HILIC-Amide consists of AMIDE and HYDROPHILIC GROUP, so that this stationary
phase is more polar than SunShell
2 an individual HILIC-Amide
group. High speed separation is leaded by core shell structure that derives
high efficiency and fast equilibration. Column:
4 SunShell HILIC-Amide, 2.6 μm 100 x 4.6 mm,
1 3
Coreshell polyol, 2.7 μm 100 x 4.6 mm,
5

Separation of Nucleic acid bases: Comparison of the other core Core shell
shellSilica,
hilic2.7columns
μm 100 x 4.6 mm
Mobile phase:
2
2 SunShell HILIC-Amide Acetonitrile/20 mM ammonium acetate(pH4.7) = 8/2
S Company
4 Column: Flow rate: 1.0 mL/min
1
1 3 3 4
5 Core shell Polyol SunShell HILIC-Amide, 2.6 μm 100
Temperature: 40 oxC4.6 mm,
5 Coreshell polyol, 2.7 μm 100 x 4.6 mm,
Detection: UV@250 nm
Core shell Silica, 2.7 μm 100 x 4.6 mm
Mobile phase:Sample: 1 = Thymine, 2 = Uracil, 3 = Uridine, 4 = Cytosine, 5 = Cytidine
22
Acetonitrile/20 mM ammonium acetate(pH4.7) = 8/2
S Company
4 Flow rate: 1.0 mL/min
1 3 Core shell Polyol
3 54
A Company Temperature: 40 oC
1 5 Detection: UV@250 nm
Core shell Silica Sample: 1 = Thymine, 2 = Uracil, 3 = Uridine, 4 = Cytosine, 5 = Cytidine
2 Regarding retention of cytidine, SunShell HILIC-Amide showed
30% higher retention factor than S core shell polyol.
3 A Company
4
1
Retention5 time/min
Core shell Silica
Regarding retention of cytidine, SunShell HILIC-Amide showed
30% higher retention factor than S core shell polyol.
Inner diameter
ORDERING INFO OF time/min
SUNSHELL 1.0 2.1 3.0 4.6 USP category
Separation of Cyanuric
Retention acid and Melamine
(mm) Separation of water- soluble vitamins
Length (mm) Catalog no Catalog no Catalog no Catalog no Catalog no
2
Separation of Cyanuric acid and Melamine Separation of water- soluble vitamins2 3
30 --- CH6931 CH6331 CH6431
2 50 --- CH6941
2 CH6341 CH6441
3 1
Sunshell HILIC-Amide, 2.6 µm 75 --- CH6951 CH6351 CH6451 L68
100 --- 1 CH6961 CH6361 CH6461
1 150 --- CH6971 CH6371 CH6471

18 1
SunShell RP Guard Filter ＜Cartridge Type, Bonded with C18 and End-Capped with TMS
Available as a guard column for reversed phase
＜Cartridge Type, Bonded with C18 and End-Capped with TMS＞
Available as a guard column for reversed phase
Tubing
0.13mmID, Holder Before
60mm length
Tubing SUNSHELL
0.13mmID, Holder Before
60mm length
After
Cartridge
filter

PROTECT YOUR COLUMNS After bonded

with C18
Cartridge
filter

RP GUARD
 The filter isFILTER
bonded
with C18 made of porous glass sized 4 mm i.d. and 4 mm thickness.
 Pore diameter is 2 µm.
 Low dead volume structure
 The filter is made of porous glass sized 4 mm .i.d. and
The Back 4ismm
filter madethickness.
pressure on glass
of porous filtersized
glass is ca.4 mm
0.1 MPa at 41.0
i.d. and mm mL/min of flow rate.
thickness
 Pore diameter is 2 µm.Tubing  Upper pressure
. Pore size is 2 μm limit is more than 60 MPa
Holder
 Low dead volume structure
0.13mmID,  Available
. Low dead volume for structure
2.1 mm i.d to 4.6 mm i.d. column
 Back pressure on 50mm
glasslength
filter is ca. Before
0.1 MPa at. 1.0 mL/min of flow
Back pressure on glass rate.
filter is ca 0.1 MPa at 1.0 mL/min of flow rate
 Upper pressure limit is more than 60 MPa Evaluation of SunShell
. Upper pressure limit is more RP Guard
than 60Filter
MPa
 Available for 2.1 mm i.d to 4.6 mm i.d. column. Available for 2.1 mm i.d to 4.6 mm i.d. columns
Cartridge filter SunShell C18, 2.6 μm 50 x 2.1 mm SunShell C18, 2.6 μm 150
Hex 14 mm
bonded with C18 After
Evaluation of SunShell RP Guard Filter Without Guard Filter
Without Guard Filter
tR(3)= 2.46 min Mobile phase:
SunShell C18, 2.6 μm 50 x 2.1 mm SunShell C18, 2.6= μm
N(3) 9,239150 x 4.6 mm
CH CN/H O=60/40 for 2.1 mm i.d.
3 2
CH3CN/H2O=70/30 for 4.6 mm i.d.
Without Guard Filter
Without Guard Filter Flow rate:
tR(3)= 2.46 min Mobile phase: tR(3)= 3.24 min 0.3 mL/min for 2.1 mm i.d.
N(3) = 9,239 N(3) = 39,345 1.8 mL/min for 4.6 mm i.d.
CH3CN/H2O=60/40 for 2.1 mm i.d.
CH3CN/H2O=70/30 for 4.6 mm Temperature: 25 ºC With Guard Filter
Withi.d.
tR(3) = 2.57 min
Guard Filter
Flow rate: N(3) = 8,786 Detection: UV@250nm
0.3 mL/min for 2.1 mm i.d. 5% decrease of plate Sample: 1 = Uracil

1.8 mL/min for 4.6 mm i.d. 2 = Toluene

3 = Acenaphthene
tR(3) = 2.57 min Temperature: 25 ºC With Guard Filter tR(3) = 3.26 min
With Guard Filter 4 = Butylbenzene
N(3) = 8,786 Detection: UV@250nm N(3) = 38,940
5% decrease of plate Sample: 1 = Uracil 0 1 2 3 4 Little change
5 Small change of
of plates
plate 0 1 2 3
2 = Toluene Retention time/min Retention time/mi
3 = Acenaphthene
4 = Butylbenzene

0 1 2 3 4 5 0 1 2 3 4 5
Retention time/min Price of SunShell RP Guard Filter
Retention time/min

ORDERING INFO OF SUNSHELL RP GUARD FILTER Name quantity Part

(available as a guard column for reversed phase because of C18 bonding) Holder: 1 piece,No
RP Guard filter : 1 piece
Price of SunShell RP Guard Filter SunShell RP Guard Filter Starter Kit Tubing: 1piece, Nut: 2 pieces, Ferrule: 2 CB
Sunshell RP Guard Filter Starter Kit (holder, cartridge, tubing) CGGAAKN
pieces (pressure Max: 9000 psi , 62MPa)
Name Sunshell RP Guard Filter for exchange (5 pcs) quantity Part number Photo CBGAAC
Sunshell RP Guard Filter holder CBGAAH
Holder: 1 piece,SunShell
RP GuardRPfilter
Guard: 1 Filter
piece For exchange 5 pieces CBG
SunShell RP Guard Filter Starter Kit Tubing: 1piece, Nut: 2 pieces, Ferrule: 2 CBGAAK 19
pieces (pressure Max: 9000 psi , 62MPa)
 sommar.se
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TEL: +46 (0)300 56 91 80
[email protected] www.biotech.se

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20