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SANKALP BHARAT
Molecular Basis of Inheritance Extra DPP-02

1. Purified biochemicals from heat killed S-strain 7. In Hershey and Chase experiment Bacteriophage
contain- with radiolabeled protein , after centrifugation
(1) DNA and lipid radioactivity appears in -
(2) DNA, RNA and protein (1) radioactive sulphur in pellet
(3) Nucleic acid and lipid (2) radioactive phosphorus in supernatant
(4) All (3) radioactive sulphur in supernatant
(4) radioactive phosphorus in pellet
2. Which of the following inference cannot be drawn
from experiment of Griffith- 8. If radioactivity of phosphorus is observed in
(1) Transforming principle is thermostable supernatant in Hershey and Chase experiment than
(2) Transforming principle enter into cell from which might be inference
medium (1) DNA is genetic material
(3) Transforming principle is DNA (2) RNA is genetic material
(4) Transforming principle can change expression (3) Protein is genetic material
of R-strain (4) All

3. One gene one enzyme hypothesis is given by- 9. RNA is genetic material in –
(1) Beadle and Tatum (1) QB bacteriophage
(2) Griffith (2) TMV
(3) Ingram (3) HIV
(4) Meselson and Stahl (4) all

4. Deoxyribonucleoside triphosphate serve dual 10. Which feature of genetic material is useful for
purposes. The purposes are evolution-
(1) act as substrate and decrease reaction rate (1) slow change
(2) provide energy for polymerization and act as (2) express itself
substrate (3) chemically stable
(3) decrease reaction rate and provide energy for (4) generate its replica
polymerization
(4) synthesize RNA primer and decrease reaction 11. Biomacromolecule/s which have ability to make its
Rate copies -
(1) DNA (2) RNA
5. Correct sequence in Blending experiment of (3) proteins (4) both 1 and 2
Hershey and Chase-
(1) Centrifugation, blending, infection 12. 2’-OH group in RNA have effect –
(2) Infection, blending, centrifugation (1) make it labile (2) prone to degradation
(3) Blending, infection, centrifugation (3) make it reactive (4) all
(4) Infection, centrifugation and blending
13. Mark the incorrect statement-
6. Radioisotope incorporated in protein during (1) DNA is chemically less reactive than RNA
blending experiment is- (2) DNA is structurally more stable than RNA
(1) S 35 (2) P 32 (3) DNA is not catalytically active
32
(3) I (4) none (4) RNA is catalytically inactive
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14. Which of the following is least thermostable- 23. If heat killed R-strain of Streptococcus along with
(1) DNA (2) RNA live R-strain injected in mice than which is true-
(3) protein (4) all (1) Mice die to pneumoniae and transformation
occur
15. DNA evolved from RNA by- (2) Mice alive and transformation occur
(1) chemical modification (3) Mice die due to pneumoniae and no
(2) structural change transformation occur
(3) both 1 and 2 (4) Mice die due to pneumoniae and from dead
(4) none mice live R-strain obtained

16. For transmission of character which is better- 24. Smooth strain of Streptococcus S-strain is due to-
(1) DNA (2) RNA (1) protein
(3) protein (4) all (2) mucopolysaccharide
(3) lipid
17. Which of the following process evolved around (4) nucleic acid
RNA-
(1) splicing 25. DNA is transforming principle is proved by-
(2) translation (1) Avery, McCarty, Macleod
(3) initial metabolism (2) Griffith
(4) all (3) Both 1 and 2
(4) Hersey and Chase
18. DNA resist change by evolving –
(1) double strand 26. In linear DNA of 200 base pair the number of
(2) repair phosphodiester bond is–
(3) both 1 and 2 (1) 198 (2) 201
(4) none (3) 199 (4) 398

19. Which is catalytically active- 27. Number of nucleotides in DNA is equal to –

(1) DNA (2) RNA (1) Phosphodiester linkage
(3) protein (4) both 2 and 3 (2) Covalent bonds
(3) Nitrogenous bases
20. In Hershey and Chase experiment if blending is not (4) Hydrogen bond
done-
(1) Supernatant show radioactivity of sulphur 28. Which of the following is Circular DNA–
(2) Supernatant show radioactivity of phosphorus (1) Plasmid DNA
(3) Pellet show radioactivity from both sulphur and (2) Mitochondrial DNA
phosphorus (3) Chloroplast DNA
(4) All are correct (4) All

21. Which of the following is transcriptionally inactive- 29. DNA secondary structure stability depend on –
(1) Heterochromatin (1) Number of Hydrogen bond
(2) Euchromatin (2) Number of G.C content
(3) Chromosome (3) Phosphodiester bond
(4) Both 1 and 3 (4) Both 1 and 3

22. According to Griffith transforming principle is – 30. ……………… proposed the Central dogma in
(1) DNA molecular biology-
(2) RNA (1) Francis Crick (2) Watson
(3) protein (3) Franklin (4) Wilkins
(4) unknown
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31. Central dogma in molecular biology, which states 40. Which of the following is most condensed form of
that the genetic information flows from– DNA–
(1) DNA to mRNA and then to Protein (1) Euchromatin
(2) RNA to DNA and then to Protein (2) Heterochromatin
(3) RNA to RNA and then to Protein (3) DNA
(4) DNA to RNA and then to Protein (4) Metaphasic Chromosome

32. Transcription and DNA replication have common – 41. DNA replication is semiconservative first
(1) Final product (2) Substrate experimentally proved in
(3) Template (4) All (1) E. coli (2) Plants
(3) Animals (4) Fungi
33. For Reverse transcription template is –
(1) RNA (2) DNA 42. During DNA replication, okazaki fragments are
(3) Protein (4) All used to elongate
(1) The leading strand towards replication fork
34. Reverse transcription is done by– (2) The lagging strand towards replication fork
(1) Retrovirus (3) The leading strand away from replication fork.
(2) Virus with RNA genome (4) The lagging strand away from the replication
(3) By virus with the help of reverse transcriptase fork
(4) All
43. N15 and N14 use in Meselson andStahl experiment
35. Reverse transcriptase enzyme is – differ in -
(1) DNA dependent DNA polymerase (1) N14 is radioactive and N15 is non-radioactive
(2) RNA dependent RNA polymerase (2) Both is light DNA
(3) DNA dependent RNA polymerase (3) N14 show less density than N15
(4) RNA dependent DNA polymerase (4) None of the above

36. If the length of diploid DNA double helix in a 44. Mark the correct statement-
typical mammalian cell is – (1) Meselson and Stahl experiment and experiment on
(1) 2.2 meters (2) 1.1 m Vicia faba is carried out insame year.
(3) 3.5 nm (4) 2 m (2) DNA replication is semi-conservativewas
proposed first than its experimental prove came
37. DNA of prokaryotic cell is located in– (3) DNA in chromosome also replicate semi
(1) cytoplasm conservatively proved by experimenton Vicia
(2) nucleus faba
(3) both 1 and 2 (4) All are correct
(4) ribosome
45. In Meselson & Stahl experiment after 4generation
38. Packaging of DNA occur for– number of N14. N14 DNA is............. if we start with one
(1) Prokaryotic cell cell with heavy DNA and grow in N14 source –
(2) Eukaryotic cell (1) 14 (2) 6
(3) Both 1 and 2 (3) 30 (4) None of these
(4) Virus
46. If generation time of E. coli is 20min and if we start
39. In which phase of cell cycle in Eukaryotes DNA is with 16 number of E. coli having light DNA and grow
most condensed – it on heavynitrogen source for 80 min calculate cell
(1) M-phase (2) Metaphase having hybrid DNA –
(3) S-phase (4) G1 phase (1) 240 (2) 256
(3) 254 (4) 32
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47. In Meselson & Stahl experiment, percentage of hybrid 55. Which is correct about enzyme which produce primer
DNA after fivegeneration- during DNA replication-
(1) 12.5 percent (2) 6.25 percent (1) It forms primer on both strand of DNA
(3) 25 percent (4) 50 percent (2) It is DNA dependent RNA polymerase
(3) Also known as primase and ribose nucleoside
48. If we obtain 12 cells with hybrid DNA after growing E. triphosphate is substrate
coli cells for five generation on N14 source, then (4) All of the above
calculate how many cells initially waspresent with
N15N15 DNA – 56. Mark the incorrect statement-
(1) 6 cells (2) 12 cells (1) Primer synthesize in 5’ to 3’ directionon both
(3) 18 cells (4) 24 cells leading and lagging strand
(2) DNA dependent polymerase always polymerize
49. If we obtain 20 cells with hybrid DNA after growing in 5’ to 3’ direction
E. coli cell on fifty generation on N14 source, then (3) Leading strand show DNA replicationin 5’ to 3’
calculatehow many cells initially was present with direction and lagging in 3’ to 5’ direction
N15N15 DNA - (4) Ligase catalyzed phosphodiester bond formation
(1) 2050 (2) 10
(3) 23 (4) 50 57. Enzyme which break H-bond to generate replication
fork within replicon is –
50. In a culture plate if N14 source was present, after 80 (1) helicase
min twenty bacteria was hybrid, then how many (2) topoisomerase
bacteria ispresent in culture plate with N14N14 DNA at (3) ligase
same time - (4) DNA polymerase
(1) 140 (2) 160
(3) 200 (4) 100 58. Which is incorrect statement-
(1) Okazaki fragments are present in lagging strand
51. In Taylor experiment for semiconservative DNA (2) Okazaki fragments are joined by ligase
replication, faba beanis grown in (3) On leading strand repeated primer synthesis
(1) Heavy thymidine occur
(2) Radioactive thymidine (4) On lagging strand repeated primer synthesis
(3) Radioactive adenine occur
(4) Heavy cytidine
59. Number of replicon present inprokaryotes-
52. Which of the following is not true DNA polymerase- (1) one (2) two
(1) use template DNA (3) three (4) none
(2) substrate is dNTPs
(3) product is polynucleotide chain 60. Which of the following is not present in one replicon-
(4) can add nucleotide at 5’ end (1) one origin
(2) two terminuses
53. Which of the following can be substrate DNA (3) two replication forks
polymerase- (4) single replication fork
(1) dAMP (2) UTP
(3) dGDP (4) dcTP 61. Mark the incorrect statement -
(1) Replication fork moves away from origin
54. Which of the following enzyme not require in DNA (2) Replication fork move towards terminus
replication- (3) Lagging stand DNA replication occur away from
(1) RNA polymerase replication fork
(2) Ligase (4) Leading strand DNA replication occur away from
(3) Endonuclease replication fork
(4) DNA dependent DNA polymerase
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62. Which is correct about Primase- 70. Who experimentally proved the semiconservative
(1) it is DNA polymerase mode of DNA replication?
(2) it is RNA polymerase (1) Mathew Meselson
(3) it is DNA dependent RNA polymerase (2) Franklin Stahl
(4) both 1 and 3 (3) Both 1 and 2
(4) Watson and Crick
63. DNA replication is differed fromtranscription in -
(1) both occur in 5’ to 3’direction 71. Similar experiments like Meselson and Stahl was
(2) both require DNA as template performed by Taylor in 1958. The experimental
(3) nucleoside triphosphate is substrate organism of Taylor was
(4) both require origin (1) Vicia faba (2) Fungi
(3) E. coli (4) Protista
64. Mark the incorrectly matched-
(1) Helicase- break H-bond 72. Radioisotope used by Taylor in his experiment was
(2) SSB - keep DNA single stranded (1) iron (2) titanium
(3) Topoisomerase- decrease DNA negative (3) thymidine (4) copper
supercoiling
(4) DNA ligase- link single nucleotide with 73. DNA polymerase is
polynucleotide chain (1) DNA dependent (2) DNA independent
(3) RNA dependent (4) RNA independent
65. Which is correct-
(1) Replicon of prokaryotes is smaller thaneukaryotic 74. DNA polymerization rate of DNA polymerase is
replicon (1) 1000 bp/s (2) 2000 bp/s
(2) Eukaryotes replicon number is more than one (3) 3000 bp/s (4) 5000 bp/s
(3) All replicon start replication once in a cell cycle
(4) DNA replication of prokaryotes is slower than 75. For long DNA molecules, the two strands of DNA
eukaryotes cannot be separated in its entire length due to the
requirement of
66. IF in one replication fork have total number of Okazaki (1) enzymes
fragments are 10 thantotal number of primers (2) high energy
synthesize in replicon are- (3) RNA
(1) 20 (2) 22 (4) phosphate and nucleotide
(3) 20 (4) 5
76. Replication occurs within the small opening of DNA
67. Which of the following process occuronce in a cell helix referred to as
cycle- (1) replication fork (2) duplication fork
(1) DNA replication (2) transcription (3) DNA fork (4) RNA fork
(3) translation (4) all
77. DNA-dependent DNA polymerases catalyzes
68. Which of the following undergo replication – polymerization in which direction?
(1) origin (2) terminus (1) 3' → 5' (2) 5' → 2'
(3) heterochromatin (4) all (3) 5' → 3' (4) 2' → 5'

69. Which of the following is true- 78. On which strand of DNA, replication is continuous?
(1) In one replicon two leading and two lagging (1) 5' → 3' polarity strand
strand presents (2) 3' → 5' polarity strand
(2) One replicon has two terminus and one origin (3) 3' → 2' polarity strand
(3) Two replication forks generated at origin and (4) 3' → 4' polarity strand
move away from it
(4) All are true
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Answer Key
1. (2) 27. (3) 53. (4)
2. (3) 28. (4) 54. (3)
3. (1) 29. (4) 55. (4)
4. (2) 30. (1) 56. (3)
5. (2) 31. (1) 57. (1)
6. (1) 32. (3) 58. (3)
7. (3) 33. (1) 59. (1)
8. (3) 34. (4) 60. (4)
9. (4) 35. (4) 61. (4)
10. (1) 36. (1) 62. (4)
11. (4) 37. (1) 63. (4)
12. (4) 38. (3) 64. (4)
13. (4) 39. (2) 65. (2)
14. (3) 40. (4) 66. (2)
15. (3) 41. (1) 67. (1)
16. (1) 42. (4) 68. (4)
17. (4) 43. (3) 69. (4)
18. (3) 44. (4) 70. (3)
19. (4) 45. (1) 71. (1)
20. (3) 46. (4) 72. (3)
21. (4) 47. (2) 73. (1)
22. (4) 48. (1) 74. (2)
23. (1) 49. (2) 75. (2)
24. (2) 50. (1) 76. (1)
25. (1) 51. (2) 77. (3)
26. (4) 52. (4) 78. (2)