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Parts of A Microscope and Their Functions 1

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9 views

Parts of A Microscope and Their Functions 1

Uploaded by

glezilolog86
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Parts of a Microscope and their functions

• Eyepiece (Ocular Lens): The lens at the top of the microscope that you look
through, usually with a
magnification of 10x or 15x.
• Arm: The part of the microscope that connects the base to the head and the
eyepiece tube. It is
used to carry the microscope.
• Stage Clips: Metal clips on the stage that hold the slide in place.
• Coarse Adjustment Knob: A larger knob used for focusing the microscope. It
moves the stage or the
body tube up and down to bring the specimen into general focus.
• Fine Adjustment Knob: A smaller knob used for fine-tuning the focus of the
specimen after using
the coarse adjustment knob. It moves the stage slightly to sharpen the image.
• Draw Tube: The tube that connects the eyepiece to the microscope body.
• Revolving Nosepiece: The part that holds two or more objective lenses and
can be rotated to easily
change power (magnification).
• Objective: The lenses closest to the specimen, that are typically of varying
magnifications (e.g., 4x,
10x, 40x, 100x).2t
• Stage: The flat platform where the slide is placed for observation.
• Diaphragm (Iris): A rotating disk under the stage with different sized holes. It
is used to vary the
intensity and size of the cone of light that is projected upward into the slide.
• Illuminator: A light source located at the base of the microscope.
• Base: The bottom part of the microscope that provides stability and support. It
houses the
illuminator and other electrical components.

How to handle a compound microscope


1. Turn the revolving nosepiece so that the lowest power objective lens (eg. 4x)
is clicked into
position.
2. Place the microscope slide on the stage and fasten it with the stage clips.
3. Look at the objective lens and the stage from the side and turn the focus
knob so the stage moves
upward. Move it up as far as it will go without letting the objective touch the
coverslip.
4. Look through the eyepiece and move the focus knob until the image comes
into focus.
5. Adjust the condenser and light intensity for the greatest amount of light.
6. Move the microscope slide around until the sample is in the center of the field
of view.
7. Use the focus knob to place the sample into focus and readjust the condenser
and light intensity
for the clearest image.
8. When you have a clear image of your sample with the lowest power objective,
you can change to
the next objective lenses. You might need to readjust the sample into focus
and/or readjust the
condenser and light intensity. Do not let the objective lens touch the slide!
9. When finished, lower the stage, click the low power lens into position and
remove the slide.

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