Chlyah, Omar, Meriem Alaoui Mdarhi, Douae El Ghoubali, Michael Kieras, Stacy Pirro,
and Hassan Ghazal. 2024. “The Complete Genome Sequence of Ceratonia Siliqua L.
(Fabaceae, Fabales), Carob.” Biodiversity Genomes, January.
https://doi.org/10.56179/001c.92058.
GENOME SEQUENCING
The Complete Genome Sequence of Ceratonia siliqua L. (Fabaceae,
Fabales), Carob
Omar Chlyah1, Meriem Alaoui Mdarhi2, Douae El Ghoubali3, Michael Kieras4, Stacy Pirro4, Hassan Ghazal3,4,5
1Laboratory of Plant Physiology and Biotechnology, Mohammed V University in Rabat, 2 Biotechnology Unit, National Institute of Agricultural Research,
3Laboratory of Genomics, Bioinformatics and Digital Health, Mohammed VI University of Science and Health, 4 Biodiversity, Iridian Genomes, 5 Department of
Sports Sciences, Laboratory of Sports Sciences and Performance Optimization, Royal Institute of Executive Management for Youth and Sport
https://doi.org/10.56179/001c.92058
Biodiversity Genomes
We present the whole genome sequence of Ceratonia siliqua L. Illumina paired-
end reads were assembled by a de novo method followed by a finishing step. The
raw and assembled data are publicly available via GenBank: Sequence Read
Archive (SRR24502586) and assembled genome (JASKGM000000000).
Introduction
Ceratonia siliqua L., or carob, is a flowering evergreen tree or shrub in the
legume family, Fabaceae. It is widely cultivated for its edible fruit pods, and as
an ornamental tree in gardens and landscapes. The carob tree is native to the
Mediterranean region and the Middle East. Portugal is the largest producer of
carob, followed by Italy and Morocco (Brassesco et al. 2021).
In the Mediterranean Basin, extended to the southern Atlantic coast of
Portugal, carob pods are often used as animal feed. The ripe, dried, and
sometimes toasted pods are often grounded into carob powder, which can be
used as an alternative to cocoa powder.
Methods
A single leaf from a Moroccan cultivated tree was used for this study. DNA
extraction was performed using the Qiagen DNAeasy genomic extraction kit
using the standard process. A paired-end sequencing library was constructed
using the Illumina TruSeq kit, according to the manufacturer’s instructions.
The library was sequenced on an Illumina Hi-Seq platform in paired-end, 2
× 150bp format. The resulting fastq files were trimmed of adapter/primer
sequence and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse,
and Usadel 2014). The trimmed sequence was assembled by SPAdes v2.5
(Bankevich et al. 2012) followed by a finishing step using Zanfona v1.0 (Kieras
2021) to make additional contig joins based on conserved regions in related
species.
Data availability
Raw reads (SRR24502586) and the assembled genome (JASKGM000000000)
are available in Genbank.
The Complete Genome Sequence of Ceratonia siliqua L. (Fabaceae, Fabales), Carob
Funding
Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345
Genomic Studies of Eukaryotic Taxa
Conflict of Interest Statement
The authors declare they have no conflicts of interests.
Submitted: January 04, 2024 EST, Accepted: January 06, 2024 EST
Biodiversity Genomes 2
The Complete Genome Sequence of Ceratonia siliqua L. (Fabaceae, Fabales), Carob
references
Bankevich, Anton, Sergey Nurk, Dmitry Antipov, Alexey A. Gurevich, Mikhail Dvorkin, Alexander
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Applications to Single-Cell Sequencing.” Journal of Computational Biology 19 (5): 455–77. http
s://doi.org/10.1089/cmb.2012.0021.
Bolger, Anthony M., Marc Lohse, and Bjoern Usadel. 2014. “Trimmomatic: A Flexible Trimmer for
Illumina Sequence Data.” Bioinformatics 30 (15): 2114–20. https://doi.org/10.1093/bioinformatic
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Brassesco, María Emilia, Teresa R.S. Brandão, Cristina L.M. Silva, and Manuela Pintado. 2021.
“Carob Bean (Ceratonia Siliqua L.): A New Perspective for Functional Food.” Trends in Food
Science & Technology 114: 310–22. https://doi.org/10.1016/j.tifs.2021.05.037.
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b.com/zanfona734/zanfona.
Biodiversity Genomes 3
