SHEEP HEART DISSECTION

Purpose
To review the structural characteristics of the human heart and to examine the major features of a mammalian heart.

Materials
 Preserved sheep heart  Dissecting pan  Tweezers
 Dropper  Scalpel  Gloves (if desired)
 Red food color  Probe
 Blue food color  Scissors

Procedure A—The Human Heart

1. Using your notes and wonderful memory, label the diagram of the human heart on your lab report sheet.

2. Read through the analysis questions on your lab report as you should be answering them as a groups as you dissect
the sheep heart.

Procedure B—Dissection of a Sheep Heart

1. Obtain a preserved sheep heart. Rinse it thoroughly in water to remove as much of the preservative as
possible. Also run water into the larger blood vessels to force any blood clots out of the heart chambers.

2. Locate the aorta on the superior surface of the heart. Fill your dropper half way with RED food coloring. Insert your
dropper into the aorta and inject the food coloring.

3. Locate the pulmonary artery on the superior surface of the heart. Fill your dropper half way with BLUE food coloring.
Insert your dropper into the pulmonary artery and inject the food coloring.

4. Place the heart in a dissecting tray with its anterior surface up (See Figure 1 below). Locate the interventricular sulcus
and label it with a solid black line. Have Mrs. Chevalier check to make sure you have labeled this correctly.

a. Locate the visceral pericardium, which appears as a thin, transparent layer on the surface of the
heart. Use a scalpel to remove a portion of
this layer and expose the myocardium beneath.
Also note the abundance of fat along the paths
of various blood vessels. This adipose tissue
occurs in the loose connective tissue that
underlies the visceral pericardium.

b. Identify the following:

o right atrium FIGURE 1
o right auricle
o right ventricle
o left atrium
o left auricle
o left ventricle

5. Examine the posterior surface of the heart (Figure 2 below).

Locate the coronary sulcus and label it with a solid black line.
Have Mrs. Chevalier check to make sure you have labeled this
correctly before proceeding.
6. Place the heart on its side so that the right ventricle is resting on your dissection tray and the apex of the heart is
pointed toward you. The anterior side of the heart should be on your left and the posterior side of the heart should
be on your right. Make a “mock” incision bisecting the anterior and posterior sides of the heart.

7. Flip the heart onto its other side so that the left ventricle is resting on your dissection tray and the apex of the heart is
pointed away from you. The anterior side of the heart should be on your right and the posterior side of the heart
should be on your left. Make a “mock” incision bisecting the anterior and posterior sides of the heart. Have Mrs.
Chevalier check your “mock” incisions before making any cuts.

8. Once both of your “mock” incision have been checked, carefully cut into the heart. You want to penetrate
the entire depth of the heart wall, but no further.

9. Open the right atrium. There are three vessels that enter
the right atrium: the superior vena cava, the inferior vena
cava, and the coronary sinus.
a. Place your probe through each of the three vessels
to see where blood enters the heart.

b. Locate the tricuspid valve.

10. Open the right ventricle.

a. Locate the chordae tendinae and papillary muscles.

b. Use your probe to trace the flow of blood through

the pulmonary trunk. If you correctly identified this
at the beginning of the lab, this should be died BLUE.

c. Examine the pulmonary semilunar valve.

11. Open the left atrium.

a. Locate the four openings of the pulmonary veins.
Pass your probe through each opening and locate
the stump of its vessel on the exterior of the heart.

b. Examine the bicuspid valve (mitral valve).

12. Open the left ventricle.

FIGURE 2
a. Compare the thickness of the left ventricular wall with that of the right ventricle.

b. Locate the chordae tendinae and papillary muscles.

c. Use your probe to trace the flow of blood through the aorta. If you correctly identified this at the beginning
of the lab, this should be died RED.

d. Compare the thickness of the aortic wall with that of the pulmonary trunk.

e. Examine the aortic semilunar valve.

13. Place your heart and all of the chunks back into the bag the heart came in. Place the bag in the trash.

14. Clean up your table, tray, and tools.

 15. Get Mrs. Chevalier’s signature after showing her your cleaned table, tray, and tools. ______________

Names ___________________________

Sheep Heart Dissection Lab ___________________________

Period _____
Please label the diagram of the human heart below.
1
2 1___________________________________
9 2. __________________________________
3. __________________________________
3
4. __________________________________
10
5. __________________________________
5 11
6. __________________________________
12
4 7. __________________________________
8. __________________________________
13 9. __________________________________
6 10. _________________________________
14
11. _________________________________
12. _________________________________
7
13. _________________________________
8
14. _________________________________
15 15. _________________________________

Analysis Questions
1. How can you tell which side of the heart is the anterior surface and which side is the posterior surface?

2. How many chambers are found in the mammalian heart? List these chambers in the order in which blood
flows through them.

3. Which chambers are the pumping chambers of the heart?

4. Which chambers are the receiving chambers of the heart?

5. From where in the body does the blood entering the superior vena cava come? Inferior vena cava?

6. How do the walls of the atria compare with the walls of the ventricles and why are they different?

7. How do the walls of the right ventricle compare with the walls of the left ventricle? Why are they different?

8. What is the purpose of heart valves?

9. Name and compare the heart valves found between the upper & lower chambers of the right and left sides
of the heart.

10. Vessels that carry blood away from the heart are called ___________________, while _________________
carry blood toward the heart.

11. Which artery is the largest and why?

12. What is the purpose of the coronary artery and what results if there is blockage in this vessel?

13. Can an artery carry deoxygenated blood? Please explain.

14. Using words, trace blood flow through the major blood vessels and heart, starting with deoxygenated
blood returned from the body.