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13 views2 pages

5205 en 1

Uploaded by

Melisa Nicević
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Kovac's Reagent Cat.

5205
For the detection of microbial indole.

Practical information
Aplications Categories
Confirmation Indole positive

Industry: Water / Food

Principles and uses


Kovac's Reagent is a reagent used for the detection of microbial indole.

Bacteria that possess the enzyme triptophanase are capable of cleaving and deaminating tryptophan with the production of indole. The indole test is
based on the formation of a dark red dye when the indole liberated reacts with 4-dimethylaminobenzaldehyde. Indole production is an important
characteristic in the identification of many microorganisms, especially E. coli (+) from other Enterobacteriaceae.

For the detection of indole, the microorganisms must be grown aerobically in a glucose-free environment with plenty of Tryptophan (Urea Indole Broth -
Cat. 1227; Peptone Water - Cat. 1403; SIM Medium - Cat. 1514; Tryptophan Culture Broth - Cat. 1237; MIO Medium - Cat. 1510; Indole Nitrate Medium -
Cat. 1504, Lauryl Sulfate Chromogenic Broth Cat. 1465, etc.). Peptone water is particularly suitable as a substrate in the study of indole production.

A positive reaction is denoted by the appearance of a pink-red color on the top of the tube. Negative reactions remain colorless or lightly yellow.

Formula in g/L
4-Dimethylaminobenzaldehyde (g) 50 Hydrocloric acid 37% (ml) 250
1-Butanol (ml) 750

Instructions for use


- Lightly inoculate the medium substrate with the microorganism to analize.
- Incubate 24-48 hours at 35 ºC.
- Add 4-5 drops of Kovac’s Reagent to the tube and shake gently.

Quality control
Solubility Appareance Color of the dehydrated medium Color of the prepared medium Final pH (25ºC)
w/o rests Liquid N/A N/A N/A

Microbiological test
Incubation conditions: (35 ºC / 24-48 h / anaerobic atmosphere).

Microrganisms Characteristic reaction


Salmonella typhimurium ATCC 14028 Indol (-)
Escherichia coli ATCC 25922 Indole (+), Pink-red color

Storage
Temp. Min.:2 ºC
Temp. Max.:8 ºC

Inspired by knowledge Page 1 of 2 - Revision number 1 - Date 22/10/2019 www.condalab.com


Bibliography
N. Kovacs, Eine vereinfachte Methode zum Nachweis der Indolbildung durch Bakterien., Z. Immunitats. Forsch. Exp. Ther., 55, 311 (1928).
Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II, & III. American Society for Microbiology, Washington, D.C.
H.H. Gadebusch, S. Gabriels, Modified Stable Kovacs’s Reagent for detection of Indol., Am. J. Cli. Pathol., 26, 1373 (1956).

Inspired by knowledge Page 2 of 2 - Revision number 1 - Date 22/10/2019 www.condalab.com

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