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3 Spectro

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Axolade Morrigan
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10 views18 pages

3 Spectro

Uploaded by

Axolade Morrigan
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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LEARNING OBJECTIVES

At the end of the learning activity the students shall be able to:
3. Identify various metal ions based on the characteristic colors they emit
when heated in a flame.
4. Understand the concept of the emission spectrum and the electronic
transitions responsible for the characteristic flame colors of different metal
ions.
5. Develop proper laboratory techniques and safety practices for conducting
flame tests, including the use of clean equipment and handling chemicals
safely.
DEFINITION
SPECTROSCOPIC
➢ Spectroscopy METHODS
– Is the study of interaction OF ANALYSIS
between mater and
electromagnetic radiation
➢ Historically, spectroscopy originated through the study of visible light
dispersed according to its wavelength by prism or grating
➢ PRINCIPLE: based on the interaction between light and matter. When
matter absorbs light, it undergoes excitation and de-excitation, which
results in the production of a spectrum. The absorption spectrum of a
material depends on its atomic and molecular composition, and the
frequency of light radiation it absorbs depends on the energy difference
between the two energy states of its molecules
TYPES OF SPECTROSCOPY
TYPES OF SPECTROSCOPY
✓ Infrared (IR) spectroscopy - Uses photons in the infrared area of
the electromagnetic spectrum to study molecules' vibrational and
rotational modes
✓ Raman spectroscopy - Analyzes vibrational, rotational, and other
low-frequency modes in a system, and is commonly used in
chemistry to identify molecules
✓ UV-Vis spectroscopy – Measures electron spectra generated
during transitions between the energy levels of valence electrons in
the spectral range of 200–750 nm
TYPES OF SPECTROSCOPY
TYPES OF SPECTROSCOPY
✓ Nuclear magnetic resonance (NMR) spectroscopy - Studies
metabolites as they're formed in the body of an organism by relying
on the magnetic resonance of atomic nuclei placed under very
strong magnetic fields
✓ Atomic absorption (AA) spectroscopy - Uses the absorption of
light to measure the concentration of gas-phase atoms, and can be
used to determine chemical elements qualitatively and
quantitatively
TYPES OF SPECTROSCOPY
Colorimetry - measurement of the wavelength and the intensity of
electromagnetic radiation in the visible region of the spectrum.
It is used extensively for identification and determination of
concentrations of substances that absorb light.

PRINCIPLE: based on the photometric technique that when a beam of


light passes through a colored solution, some of the light is absorbed,
some is reflected, and the rest is transmitted
PARTS OF SPECTRO
LABORATORY ACTIVITY NO.
5
SPECTROPHOTOMETRY
ELECTROMAGNETIC RADIATION

FREQUENCY - The number of


FREQUENCY - The number of
oscillations of an
oscillationswave
electromagnetic of anper
electromagnetic
second (ν).wave per
second (ν).
WAVELENGTH - The distance
between any two consecutive
maxima or minima of an
electromagnetic wave (λ).

WAVENUMBER - wavenumber
The reciprocal of wavelength (–
WAVENUMBERν) - wavenumber
The reciprocal of wavelength (–
ν)
COMPLEMETARY COLORS
COMPLEMETARY COLORS
SPECTROPHOTOMETRY
BEER-LAMBERTS LAW
BEER-LAMBERTS LAW
SPECTRO MACHINE
SPECTROPHOTOMETRY

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