Mr. RAMESH ROKADE Reference: Dr.

SANDEEP SAKPALE VID: 112190048612

GHATKOPAR, CHEMBUR Sample Collected At: Registered On:
Tel No : 9920969864 Apsc-hemali shah 01/06/2021 12:58 PM
PIN No: 400077 Shop no 7 laxmi palace bldg plot no 169, r Collected On:
b mehta marg mumbai - 400077. zone: c-
PID NO: P112101493429 01/06/2021 12:59PM
13c(09)
Age: 64.0 Year(s) Sex: Male PROCESSING LOCATION:- Metropolis Reported On:
Healthcare Ltd, Unit No. 409- 416, 4th 04/06/2021 10:53 AM
Floor, Commercial Building-1,
Kohinoor Mall, Mumbai-70

CULTURE & SENSITIVITY, AEROBIC BACTERIA SPUTUM

Microscopy Result
Specimen : Sputum

Gram Stain : Pus cells: >25/Low power field, epithelial cells: <10/Low power field, plenty
Gram positive cocci in chains, plenty Gram positive budding yeast cells with
pseudohyphae seen.

Final Report
Organism(s) Isolated : Candida albicans

Comments : Kindly correlate clinically & if indicated clinically take entry in Fungal Antibiogram
(F0065) within 3 days.

Dr. Ashwini Mankar

MD MICROBIOLOGY
Page 1 of 5
 Mr. RAMESH ROKADE Reference: Dr.SANDEEP SAKPALE VID: 112190048612
GHATKOPAR, CHEMBUR Sample Collected At: Registered On:
Tel No : 9920969864 Apsc-hemali shah 01/06/2021 12:58 PM
PIN No: 400077 Shop no 7 laxmi palace bldg plot no 169, r Collected On:
b mehta marg mumbai - 400077. zone: c-
PID NO: P112101493429 01/06/2021 12:59PM
13c(09)
Age: 64.0 Year(s) Sex: Male PROCESSING LOCATION:- Metropolis Reported On:
Healthcare Ltd, Unit No. 409- 416, 4th 04/06/2021 10:53 AM
Floor, Commercial Building-1,
Kohinoor Mall, Mumbai-70

Method : Culture on routine Culture medium, identification and sensitivity by automated Vitek MS / Vitek2 & conventional methods.

Note:
1. Result of culture and antimicrobial susceptibility test need to be correlated clinically.
2. Previous history of antibiotic usage may influence the growth of microorganisms in vitro.

Biological Reference interval:

• No growth or Normal flora grown.

• Identification of the probable pathogen.
• Breakpoints (also known as "clinical breakpoints") are used to categorize an organism as susceptible, susceptible-dose
dependent, intermediate, resistant, or nonsusceptible according to the Clinical and Laboratory Standards Institute (CLSI)
guidelines.
• For each organism-antimicrobial agent combination, the Clinical and Laboratory Standards Institute provides interpretive
criteria for determining whether the MIC should be interpreted as susceptible, susceptible dose dependent, intermediate,
nonsusceptible, resistant, or Epidemiological Cutoff Value (ECV).
• In some instances an interpretive category cannot be provided based on available data and the following comment will be
included: "There are no established interpretive guidelines for agents reported without interpretations."

Indications: To identify and support the diagnosis of respiratory bacterial infections including bacterial causes of pneumonia.

Interpretation & Limitations:

• Common bacterial causes of respiratory tract infection inclue Streptococcus pneumonia,Moraxella catarrhalis, Haemophilus
influenzae, Haemophilis parainfluenzae, Group A streptococci, Staphylococcus aureus, Pseudomonas aeruginosa,
Burkholderia species, Gram negative bacteria ( eg,Enterobacteriaceae especially in Nosocomial infections) and other gram
negative bacteria.
• No growth or no significant growth does not rule out all causes of respiratory infectious diseases.
• All results have to be correlated clinically in the context of clinical scenario, results of other tests including imaging
investigations, epidemiological factors.
• Some bacterial organisms (eg, Mycoplasma pneumoniae, Legionella species, mycobacteria) which cause lower respiratory
infections are not detected by this assay and require special procedures.
• In certain scenarios where the bacterial culture is negative, one may consider further/ additional testing to rule out other
bacterial, viral, parasitic or fungal infections.

Associated tests :

1. Culture and sensitivity,aerobic bacteria Blood. Test code C0182

2. Fungal Culture sputum. Test code. F0092
3. Filmarray Pneumonia panel lower respiratory (Pneumonia panel).
4. Flu panel Viral test code F0105

References:

1. In Chapter 53, 54. Respiratory Tract Infections, Upper Respiratory Tract Infections and Other Infections of the Oral Cavity
and Neck. Forbes B.A, Sahm D.H., Weissfeld AS. Bailey & Scott’s Diagnostic Microbiology, 12th Edn. St. Louis, Mosby, Inc.
2007.
2. CLSI: Performance Standards for Antimicrobial Susceptibility Testing. CLSI supplement M100. Wayne, PA: Clinical and
Laboratory Standards Institute
3. Procop GW, Church DL, Hall GS, et al. Koneman’s Color Atlas and Textbook of Diagnostic Microbiology. Seventh edition.
Philadelphia: Wolters Kluwer Lippincott Williams and Wilkins; 2017. Chapter 2, Introduction to Microbiology Part II:
Guidelines for the Collection, Transport, Processing, Analysis, and Reporting of Cultures from Specific Specimen Sources; p.
66-110.

Dr. Ashwini Mankar

MD MICROBIOLOGY
Page 2 of 5
Mr. RAMESH ROKADE Reference: Dr.SANDEEP SAKPALE VID: 112190048612
GHATKOPAR, CHEMBUR Sample Collected At: Registered On:
Tel No : 9920969864 Apsc-hemali shah 01/06/2021 12:58 PM
PIN No: 400077 Shop no 7 laxmi palace bldg plot no 169, r Collected On:
b mehta marg mumbai - 400077. zone: c-
PID NO: P112101493429 01/06/2021 12:59PM
13c(09)
Age: 64.0 Year(s) Sex: Male PROCESSING LOCATION:- Metropolis Reported On:
Healthcare Ltd, Unit No. 409- 416, 4th 04/06/2021 10:53 AM
Floor, Commercial Building-1,
Kohinoor Mall, Mumbai-70

Dr. Ashwini Mankar

MD MICROBIOLOGY
Page 3 of 5
 Mr. RAMESH ROKADE Reference: Dr.SANDEEP SAKPALE VID: 112190048612
GHATKOPAR, CHEMBUR Sample Collected At: Registered On:
Tel No : 9920969864 Apsc-hemali shah 01/06/2021 12:58 PM
PIN No: 400077 Shop no 7 laxmi palace bldg plot no 169, r Collected On:
b mehta marg mumbai - 400077. zone: c-
PID NO: P112101493429 01/06/2021 12:59PM
13c(09)
Age: 64.0 Year(s) Sex: Male PROCESSING LOCATION:- Metropolis Reported On:
Healthcare Ltd, Unit No. 409- 416, 4th 04/06/2021 10:53 AM
Floor, Commercial Building-1,
Kohinoor Mall, Mumbai-70

ROUTINE EXAMINATION SPUTUM

Specimen : SPUTUM

Physical Examination
Quantity : 2 ml

Color : Whitish

Consistency : Mucoid

Microscopy Result
Pus Cells : > 25 / Low power field

Epithelial Cells : < 10 / Low power field

Gram`s Stain : Plenty Gram positive cocci in chains, plenty Gram positive budding yeast cells
with pseudohyphae seen.
ZN Stain : No Acid fast bacilli seen

Dr. Ashwini Mankar

MD MICROBIOLOGY
Page 4 of 5
 Mr. RAMESH ROKADE Reference: Dr.SANDEEP SAKPALE VID: 112190048612
GHATKOPAR, CHEMBUR Sample Collected At: Registered On:
Tel No : 9920969864 Apsc-hemali shah 01/06/2021 12:58 PM
PIN No: 400077 Shop no 7 laxmi palace bldg plot no 169, r Collected On:
b mehta marg mumbai - 400077. zone: c-
PID NO: P112101493429 01/06/2021 12:59PM
13c(09)
Age: 64.0 Year(s) Sex: Male PROCESSING LOCATION:- Metropolis Reported On:
Healthcare Ltd, Unit No. 409- 416, 4th 04/06/2021 10:53 AM
Floor, Commercial Building-1,
Kohinoor Mall, Mumbai-70

AFB RAPID CULTURE BY BACTEC MGIT SPUTUM

Microscopy Result
Specimen : Sputum

Method : Automated Bactec Mgit 960 System

ZN Stain : No Acid fast bacilli seen.

Note : 1. Interim growth status reported at 10 days and 3 weeks of receipt of specimen.
2. Positive culture reported immediately.
3. Negative culture (Final) reported at 42 days of receipt of specimen.

Principle :

BACTEC MGIT 960 system is designed and optimized for the rapid detection of mycobacteria and continuous monitoring using non
radiometric fluorescence technology. Microorganisms present in specimens metabolize nutrients and oxygen in the culture tube. The
culture tubes contain a fluorescent sensor that responds to the concentration of oxygen in the culture medium. The instrument’s
photo detectors measure the level of fluorescence, which corresponds to the amount of oxygen consumed by organisms.

Associated tests :

1. AFB – Xpert panel : For rapid identification of M.tuberculosis complex and detection of Rifampicin resistance.
2. AFB MDR- screen Hain's Line probe assay from all pulmonary Specimen (smear positive and Negative).
3. AFB- XDR-screen Hain's Line probe assay for patients diagnosed as MDR-TB.

Metropolis Tuberculosis Laboratory Mumbai is certified by the National Mycobacteriology Certification System of Central
TB, GOI for Tuberculosis Drug Susceptibility testing by Liquid Culture (First Line) and Line Probe assay. Faculty
Certification number: 11/RNTCP/2014 & 42/RNTCP/2014.

-- End of Report --

Dr. Ashwini Mankar

MD MICROBIOLOGY
Page 5 of 5