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Unit 3 Note Set 4 AP Biology Photosynthesis Part 2

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20 views3 pages

Unit 3 Note Set 4 AP Biology Photosynthesis Part 2

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vidushibhandari22
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© © All Rights Reserved
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AP Biology Unit 3: Note Set 4 Photosynthesis – Part 2

(Associated Learning Objectives: 1.15, 1.16, 2.2, 2.5, 2.13, 4.4, 4.5, 4.17)

Important Concepts from previous unit:


1) Surface area of membranes is important to all cells, prokaryotic or eukaryotic.
2) Valence shell electrons are the important electrons when considering bonds and energy.
3) Energy absorbed by electrons causes them to move farther away from the nucleus, maybe even escape.

I. Light Reaction of Photosynthesis


A. This process is used for turning sunlight into usable chemical energy. Those molecules are: ATP and NADPH
B. The process has two parts:
1. Non-cyclic electron flow – This part makes ATP (by non-cyclic photophosphorylation) and NADPH.
a. These electons start out in water, then they are released to Photosystem II as water lyses,
the electrons then make their way to Photosystem I by way of the primary electron
transport chain, and then ultimately end up going to NADPH. This is referred to as
non-cyclic because the starting point for the electrons is different from the ending point.

2. Cyclic electron flow – This part makes extra ATP. (by cyclic photophosphorylation)
a. These electrons start out at Photosystem I and then go down the secondary electron
transport chain to Fd (Ferrodoxin), but then are transferred over to the other path by going
to Cytochrome C. They then make their way back to their starting point, Photosystem I by
way of the primary electron transport chain. Hence the term cyclic, the starting point and
ending point are the same.

C. These two parts are occurring, in the presence of sunlight, at the same time on the Thylakoid membranes.
D. There are thousands of these Photosystems (I and II) on each Thylakoid membrane. (Surface Area? It’s
important again. More surface more photosystems  more energy production.)

II. The major steps to the light reaction of Photosynthesis:


Step 1: Sunlight hits the water in the stroma and also the Photosystems I and II at the same time.
A. The water in the stroma, using the high quality E of sunlight, lyses into O gas (a waste product), 2 H+ ions
(these stay in the stroma), and 2 free electrons. (These will be used to replace the 2 “excited” electrons lost
from the Mg atom of Chlorophyll A in Photosystem II.)
B. The Photosystem II Mg atom loses two electrons due to absorbing all of the E being funneled into Chlorophyll
A from Chlorophyll B and the Carotenoids. (These 2 excited electrons are collected by a primary acceptor
protein, also in the Thylakoid membrane, and moved toward Photosystem I along the primary electron transport
chain. They will be replaced by the 2 electrons from water lysing. This keeps the process going.)
C. The Photosystem I Mg also loses two electrons due to absorbing all that E from the Chlorophyll and
carotenoid molecules. (These are also collected by another primary acceptor protein and moved toward
NADP+ along the secondary electron transport chain. These will be replaced by the 2 electrons coming down
the chain from Photosystem II in the primary electron transport chain.)

Step 2: Excited electrons travel down the electron transport chains. (This is a series of Redox reactions. A redox reaction
is basically two molecules exchanging electrons. One molecule receives them [called Reduction] and the other
molecule loses them [called Oxidation]. Hence the combined name of Redox.) This is associated with the Law of
Conservation of Mass…” Mass is neither created nor destroyed; only transferred and transformed.”
As the excited electrons go down the electron transport chain, by going through these series of Redox reactions,
their excited kinetic E (also called Free E) is being used to power the proteins called Proton pumps. As the
electrons go down their transport chain, their excited kinetic E decreases.
A. Photosystem II electrons (P.A. Pq cyto C Pc  Photosystem I)
1. Free E of the electrons is used to actively transport H+ ions (a.k.a. called protons) into the inner
thylakoid space The H+ ion concentration [H+] goes up inside the space. This causes the pH to
decrease and become more acidic. The [H+] goes down in the stroma. The stroma becomes more
basic. As this is occurring a concentration gradient is created. A concentration gradient is a source
of
potential E now. (It would be like blowing air into a balloon. The pressure builds as more air is blown
inside the balloon. This is also an example of potential E.)
B. Photosystem I electrons (P.A.  Fd  NADP+) (Reduction occurs to create NADPH from NADP+.)(This is
the ending point for non-cyclic electron flow.)
OR
(P.A.  Fd Cyto C Pc Photosystem I) This would be for cyclic electron flow.
Remember this makes extra ATP.
C. Cytochrome C is an important molecule as ALL organisms possess it in their membrane that is used for
energy production. This supports common ancestory among ALL organisms.
1. Mitochondria and Chloroplast INNER membranes in eukaryotic organelles.
2. The plasma membrane of Prokaryotic cells.

Step 3: The trapped H+ ions, inside the Thylakoid, are released through the ATP Synthetase Complex. This is a group
of enzymes in the Thylakoid membrane that helps make ATP, by Anabolic Phosphorylation. Just look the
enzymes name. This release of kinetic H+ ions powers the phosphorylation of ADP ATP. (This would be like
the
air coming out of the blown up balloon and turning a pinwheel.
A. This Kinetic movement of H+ ions produces a LARGE AMOUNT OF ATP.
B. This is an example of Energy Coupling (Two processes working together and involving energy.)The first
process was Active transport to pump the H+ ions into the confined space of the Thylakoid, using the Proton
pump proteins, to make the concentration gradient. The second process is diffusion, The H+ ions going
from high [ ] to low [ ]. The kinetic movement of the H+ fuels the production of ATP
1. This form of energy coupling, for making ATP, is referred to as Chemiosmosis.

Step 4: ATP and NADPH will now be used to power the fixing of CO2 into sugar in Calvin Cycle.

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