Seed Unit V
Seed Unit V
UNIT V -SEED PRODUCTION IN SPECIFIC CROPS once in 7 days to prevent damping off disease. Apply 2Kg of DAP 10
days prior to pulling out of seedlings. Spray rogar or metasystax @
SEED PRODUCTION IN VEGETABLES 1ml/lit of water for pest.
Top dressing : At the time of flowering 75 kg N. Conventional method of hybrid seed production
Foliar application In this method, there are two steps generally followed viz.,
NAA @ 20 ppm 65 and 75 days after planting followed every emasculation and hand pollination. As tomato is a self-pollinated crop,
time by spraying urea at 12 kg, Super phosphate 4kg, 2 kg potassium the parental lines are maintained. The male parental lines have to be
sulphate and 200 g of micronutrient mixture dissolved in 100 lit water raised atleast 15 days in advance of raising female parent so that there
/ acre. will not be any problem for availability of pollen for hybridization in
Harvest the first truss of flowers produced by female parent. A female : male
Seeds attain maturity 30-45 days after flowering parental ratio of 5:1 is recommended. Flower buds of female parent
Harvest fully matured, red, ripened and healthy fruits. First 8 - are emasculated 12 - 14 hours before opening. Flowers of male parent
10 pickings alone should be used for seed extraction. are bagged at bud stage and are picked up in the early morning. The
Seed extraction pollen is collected in dry petridishes, as stamens are hygroscopic. The
Well-matured fruits are crushed and made into a pulp. For receptivity of stigma is 21-36 hours before opening of flowers.
every 1-kg of pulp add 20-30 ml of commercial HCL acid and keep Pollination is done generally in the morning hours between 7.00 a.m.
for 30 minutes with constant stirring. At the end of 30-minutes wash and 10.00 a.m.
the seeds 3-4 times with water and air-dry the seeds.
Grading The steps followed in the production of hybrid seeds are
12 x 12 wire mesh (2.1 mm) sieve are used. Raise the female and male parents during optimum season in
Storage the ratio of 5:1
Dry the seeds to 7-8 % moisture and treat with thiram or Select one or two well-developed flower buds of female
captan 75 % WP @ 2 g. The treated seeds can be stored upto 12 parent, which will open the next day.
months in paper bags and upto 24 months in moisture vapour proof Emasculate the flower bud in the evening by removing the
containers. anther cone using clean and pointed forceps or head pin.
Cover the emasculated flowers with butter paper cover of
HYBRID SEED PRODUCTION IN TOMATO convenient size preferably, 10 x 5 cm to prevent foreign pollen
contamination.
Tomato is predominantly a self-pollinated crop and only less Cover the flower buds of pollen parent. Use covers of different
than 5.0 per cent cross-pollination has been reported. The colours for male and female parents.
conventional method of hybrid seed production in tomato involves 2 Collect pollen from the male parent between 7.00 a.m to 10.00
basic steps viz., emasculation and hand pollination which makes a.m in a clean petridish.
hybrid seed production expensive. Dust the pollen on the receptive stigma of the emasculated
flower using camel hair brush.
After hand pollination, cover the crossed flowers with butter attractive in colour, recovery is very high, remove the external seed
paper cover and label for identification. borne pathogens and do not clog each other while drying. Seed quality
Remove all other flower buds leaving one or two crossed is also very high.Seed recovery is 0.8-1.0 %.
flowers in a truss to ensure good fruit set. (III) Alkali method
Allow the flower with cover for seven days to set fruits and By using sodium bicarbonate solution (30-35g/I of water) at
remove the cover after ensuring fruit set. 1:1 ratio of the solution and pulp for 12 hours will separate the seeds
from the pulp, which will be washed and dried under shade.
SEED EXTRACTION TECHNIQUES IN TOMATO Disadvantage is seed colour will be dull.
(IV) Mechanical extraction
Fruit Grading Tomato seeds are also extracted by using tomato seed extractor
or pulper for large scale seed extraction. The seed extraction consists
Based on fruit size and shape, true to type fruits are selected of two units operated by electric motor, one is fruit pulper or crusher
for seed extraction and large to medium sized fruits alone to be used and second one is seed and pulp separator. The whole unit is made up
for extraction of higher seed recovery. In tomato seeds are extracted of stainless steel. Here extraction is immediate, seed recovery is high
from fully ripened (reddish) fruits by different methods. They are: and pulp/juice can be further used for making byproduct like jam,
(I) Fermentation method jelly etc., The cost of seed extraction is Rs.7.5/kg.
The fruits are pulped by trampling under foot or using a pulper Seed yield: 150 kg/ha
and collect the pulp in plastic container or cement tank. The pulp is
allowed to ferment overnight. The next day seeds get separated from
the pulp. The floating fraction is removed and discarded and the BRINJAL
sinkers (due to bacterial degradation the seed is fermented and settle Season: June - July.
down in the bottom of the container) are collected, washed well and Seed rate: 450 g / ha
dried in the shade for 1-2 days and then in sun between 8 -12 noon Nursery preparation
and 2-5 pm. 15 days before sowing drench the nursery with methane
(II) Hydrocholoric acid method sodium @ 28 ml / sq. m. for controlling the nematodes, after 7 days
The fruits are pulped by trampling under foot or by using a with copper oxychloride @ 5 g / lit to prevent damping off.
pulper and collect the pulp in a plastic container or cement tank. Add Age of seedling: 30-35 days.
commercial hydrocholric acid @ 20-30ml Kg-1 of pulp and keep it for Spacing: 75 x 60 cm
20-25min with occasional stirring. The seeds get separated from the
pulp and sink to the bottom. The floaters can be removed by Fertlizers
discarding. The seeds are collected, washed well with water 3-4 times Total : 150 : 75 : 75 NPK kg / ha
and dried in shade. The advantages of this method are the seeds are Basal : 100 : 75 : 75
V.Suja M.Sc., (Agri.)
UNIT- V 4
Top dressing : Just before flowering 50 kg N/ ha. HYBRID SEED PRODUCTION IN BRINJAL
Brinjal potentially a self-pollinated crop is classified as an
often cross-pollinated crop, about 30 to 40 per cent natural cross-
Supplemental foliar application pollination occurs through insects.
NAA @ 20 ppm 65 and 75 days after planting followed every Hybrid seed production in brinjal is done by emasculation and
time by spraying urea at 12 kg, super phosphate 4kg, 2 kg potassium hand pollination. The flowers of the brinjal plant are quite
sulphate and 200 g of micronutrient mixture dissolved in 100 lit water conspicuous and emasculation is fairly easy. A ratio of 10:1 for
/ acre. female and male parental lines would be the most optimum for
efficient production of hybrid seeds. The following steps are adopted
Harvest for hybrid seed production.
Seeds attain maturity 40-45 days after flowering The symptom
of harvestable maturity is turning of the skin colour from green to Raise the female and male parents at an isolation distance of 200
bright yellow. Medium size fruits yield more quantity and quality seed m from each other as well as from other varieties. The male parent
than big or small fruits. has to be raised 15-20 days ahead of the female parent.
In the plants of female parent, select well developed plumpy long
Seed extraction styled flower buds which are going to open on the next day
Well-matured ripened fruits are cut into 4 - 6 pieces and morning (which can be identified by the prominent ovary)
softened by soaking in water overnight. On extraction the floaters are Emasculate the flower buds on the previous day evening between
removed. 3.00 and 5.00 p.m. using a needle by removing the anthredial cone
carefully. Cover the emasculated flower buds with butter paper.'
Grading Similarly on the previous day evening cover the flower of male
Sieve size : 5/ 64 round perforated metal (2.1 mm) sieve or parent from which pollen is going to be collected on the next day
BSS 12 x 12 wire mesh sieve (2.1 mm) are used. morning.
Next day morning by 6.00 p.m. collect the flower buds before
Storage opening and separate the anthers and put in a petridish covered by
Treat the seeds with either thiram or captan 75 % WP @ 2 g + glass.
200 mg. The treated seeds can be stored upto 2 years in moisture Keep the petridish against sunlight to facilitate dehiscence of
vapour proof containers. anthers and release of pollen grains.
Remove the butter paper cover on the emasculated flower and dust
the pollen over the stigmatic surface with the help of camel
hairbrush.
Rebag the flower and label. Since the stigma is receptive for 4 Seed extraction
days, remove the bags only after 8-10 days when fertilized ovary Dried fruits are taken in a gunny or cloth bag and threshed
is prominently seen. with a pliable bamboo stick or TNAU model chill seed extractor. The
After full maturity, extract the seeds. seeds are separated and graded.
On an average of 400 kg of hybrid seeds can be obtained from one
hectare. Grading
Sieve size: 8/ 64" round perforated metal sieve or 8 x 8 wire
CHILLIES mesh sieve (3.1 mm) are used.
should be 5:1. Hand emasculation and hand pollination are the most Foliar application
expensive method of hybrid seed production because of high labour DAP @ 0.5 % thrice at 10 days interval commencing with first
cost and low fruit set percentage. Therefore hand pollination without flowering enhances the yield of good quality seeds.
emasculation is done using male sterility. Hence genetic male sterility Harvest
mechanism is more economical and can be exploited for hybrid seed Seeds mature 28-30 days after anthesis. Harvest the pods when
production. The 50% heterozygous male fertile plants are removed in they dry and turn brown and develop hairline cracks along the ridges.
the female plant and 50% homozygous male sterile plants are kept for In bhendi first formed two pickings can be used for vegetable purpose
hybrid seed production. The expression of genic male sterility is and the next 6 pickings can be used for seed purpose.
affected by environment especially temperature. The male sterility
less sensitive to environmental factors should be selected for hybrid Seed extraction
seed production. Few hybrids developed in Chilli are Agni, HOE 808, Harvested pods can be dried in the sun for 2-3 days and seeds
HOE 888, CH 1, HOE 818, BSS 138, BSS 141, ARCH 228, CH 104, can be extracted in a machine thresher or by hand with pliable
Capscicum are Bharat, Early Bonty, Indira, Lario and Green gold. bamboo stick.
Grading
BHENDI Sieve size: 10/ 64" round perforated metal sieve or 6 x 6 wire
mesh sieve (4.2 mm) are used.
Season: March, April and May Upgrading; To remove the empty seeds, water flotation
technique is adopted. Air dry the seeds.
Seed rate: 8 - 10 kg / ha Storage
Seeds can be easily stored when they are dried to 8 %
Spacing: 60 x 20 cm moisture content and treated with halogen formulatioan (bleaching
powder + CaCO3 + arappu leaf powder @ 5:4:1 @ 3 g / kg or captan
Fertlizers 75% WP 4g / kg.Seeds can be stored upto 15 months in cloth bag and
Total : 60: 50: 30 NPK / ha 24 months in moisture vapour proof containers.
Basal : 40 : 50 : 30 kg NPK / ha
Top dressing : 20 : 0: 0 kg NPK / ha
1. 10 kg N / ha at first flowering
2. 10 kg N / ha 10 days after
flowering.
Spacing Grading
45 x 45 cm at 2.5 x 2 m . dip 3 seeds per pit. Grade the seeds using BSS 4 x 4 size .
Storage
Manuring Captan or thiram 4 gm/kg of seeds or halogen mixture @ 5
Apply 10 kg FYM per pit. g/kg of seeds.
above factors facilitate for harvesting higher yield of quality seeds in It is more important that nursery beds should be formed with
onion. one meter breadth. This facilitates easy weeding and proper
application of fertilizers or nutrients.
Producing quality seedlings for transplanting
Selection of quality seeds will pave way for production of Isolation distance
vigourous seedling and it is more important. Transplanting healthy 1000 m for foundation seeds
and vigourous seedlings in the main field will be useful for the 800 m for certified seeds
seedling to grow faster by utilizing the applied nutrients, develops
resistance to pest and diseases and avoid gap filling operations. Fertilizer application
Hence, proper nursery management is more important to produce
healthy vigourous seedlings. Application of 2 kg DAP (or) 6 kg urea plus 12kg super
phosphate per cent nursery will be useful for the growth of the
Seed selection seedlings. Also it will be helpful for pulling out of seedlings without
any damage to root system. Transplanting these healthy seedlings is
The quality seed should be used for getting healthy seedlings. highly useful for easy crop establishment in main field.
The crop yield to tally depends on the seed quality. In seed crop, we
should aim for higher yield without sacrificing genetic purity and it is Age of the seedling
more important. The yield and quality depend on the seed selection.
Hence, for raising seed crop we should choose breeder seed or The age of the seedling is more important for crop
foundation seeds. establishment and it also influences the crop yield. Hence, for onion
35-40 days old seedlings are found to be optimum age for
Nursery preparation and sowing transplanting.
After selection of quality seeds, it is more important to produce Production of quality onion
healthy seedlings. For raising vigourous seedlings we have to form
raised nursery beds in an area of three cents applied with 200 kg of During the first season in onion seed production we have to
decomposed farm yard manure. produce the quality bulbs. It is more important because the seed yield
and quality depend on the bulbs that are produced.
Sowing
For transplanting one acre, 2.5 - 3 kg of seeds can be sown in the
raised nursery beds formed in an area of 3 cents. On the nursery beds,
seeds should be sown in lines formed at a distance of 10 cm. After Main field preparation and management
sowing, the seeds should be covered with sand or loose soil. Then the
beds must be irrigated by immersing of the whole beds with water. Land selected for raising seed crop should be ploughed thoroughly
After germination, nursery would be irrigated once in a week. for two (or) three times. At the last plough, 20 tonnes of FYM per acre
V.Suja M.Sc., (Agri.)
UNIT- V 11
has to be incorporated into the soil. Then ridges and furrows are to be seed crop. Hence, in the onion seed crop field removal of off type
formed at a spacing of 20 Cm. plants, weeds and disease infested plants is to be done scrupulously
After forming the ridges and furrows, the recommended doses of N, P before it comes to harvest to avoid the physical contamination.
and K fertilizers has to be applied on the 1/3 rd height of ridges as a The roguing operations are to be taken at different stages namely
basal dose. Application of basal doses of fertilizers will be more vegetative stage and after harvest.
important and support for the early vigourous growth of the seedlings.
The basal fertilizer requirement per acre for onion seed crop is as Bulb harvest
follows.
Urea: 26 kg, Super: 144 kg and Potash: 19 kgs Bulb harvest may be done at 110-115 days after planting (or) a
week after the visual symptom of fallen of leaves in 50% population
Transplanting at that stage bulbs attained full maturity. The bulbs should be lifted
from the soil along with leaves. The lifted bulbs along with leaves
The healthy seedlings pull out from well maintained nursery has to should be allowed in the field (or) in the shade for curing for 3-5 days.
be used for transplanting. After the irrigation i.e. complete soaking of Then the leaves are to be cut at an height of 2.5 cm from the neck of
ridges, single seedling should be transplanted at the spacing of 10 Cm. the bulb.
From the beginning, onion field should be maintained in such way Only during second season we are doing true seed production.
that it must be free from weed. Because, weed growth will affect the The bulbs produced during June-September are being used for
quality bulb harvest. Hence, two manual weeding may be taken up at planting. The seed yield and quality depend on the technologies we
20-25 days after planting and 40-45 days after planting. This will are following during second season.
facilitate quality bulb production.
Top dressing Bulb selection and grading
Top dressing with fertilizer is more important for onion bulb
production, other wise it will not be useful for further transplanting for Based on the colour, shape and size of the bulb and
onion true seed production. Hence, 26 kg of urea may be applied as confirming to varietal characters should be selected for sowing. Then
top dress on 30th day after planting. This will be useful for vegetative the selected bulbs have to be cured for at least 15 days. The bulbs
growth and bulb growth. selected confirming varietal character should possess at least 4-6 cm
diameter. The smaller bulbs may be used for vegetable purpose.
Roguing for quality bulb production
In the main field sometime abnormal difference in growth habits The cool weather prevailing after bulb planting will be useful for good
in some plants may be noticed and this shows the admixture of some flowering. Good sun shine at the time of full blooming stage will
other varieties. This may very much affect the genetic purity of that facilitate the activity of beneficial insects for higher rate of cross
particular onion seed crop and ultimately the crop will not be fit for a pollination and seed set. The relative humidity should be lower at the
V.Suja M.Sc., (Agri.)
UNIT- V 12
time of seed development. All these factors will be there during seed. Hence, two manual weeding may be taken up at 20-25 days
October-November. planting and 40-45 days after planting. This will facilitate for
flowering production.
Bulb treatment
Top dressing
The top portion (3-5 mm) of the bulb has to be cut and removed.
Then the bulbs are to be treated with bavistin (or) dithane M45 @ 20 Top dressing with fertilizer is more important for seed
g/l0 litre of water for 5-10 minutes before sowing. This will protect production. Hence, 26 kg of urea may be applied as top dress on 30th
the bulb from soil borne pathogen until its germination. after day planting. This will be useful for good flowering and seed set.
Main field preparation and management Roguing for quality seed production
Land selected for raising seed crop should be ploughed thoroughly Already we have seen the importance of isolation distance for
for two (or) three times. At the last plough, 20 tonnes of FYM per acre quality seed crop. Now, we are going to learn how the off types and
has to be incorporated into the soil. Then ridges and furrows are to be rogues influence the quality of seed and by roguing operation how
formed at a spacing of 30 cm. the genetic purity of the variety is being maintained
After forming the ridges and furrows, the recommended doses of N, P
and K fertilizers has to be applied on the 1/3rd height of ridges as a In the main field sometime we are noticing abnormal difference
basal dose. Application of basal doses of fertilizers will be more in growth habits in some of the plants and this shows the admixture
important and support for the early vigourous growth of the seedlings. of some other varieties. This may very much affect the genetic purity
The basal fertilizer requirement per acre for onion seed crop is as of that particulars onion seed crop and ultimately the crop will not be
follows. fit for a seed crop. Hence, in the onion seed field, removal of off type
Urea: 26 kg, Super: 144 kg and Potash: 19 kgs plants, weeds and disease infested plants is to be done scrupulously
before it comes to harvest to avoid the genetic contamination.
Planting
In roguing operations are to be taken at different stages namely
Treated bulbs alone must be used for planting. After complete vegetative stage, flowering and after harvest.
soaking of ridges, the bulbs are to be planted at 10 cm spacing. The
women are playing major role in bulb planting. The field should be Harvest
again irrigated three days after planting. This facilitate for easy
germination of bulbs. Harvest must be done only after complete maturation of seed. The
Weed management best time of harvest is the 50% of the seeds in the umbel is exposed.
At the stage umbels alone should be harvested in 3 to 4 pickings at an
From the beginning, the field should be maintained from free interval of 45 days. The harvested umbels have to be dried under
of weeds, otherwise the weed competition will reduce the quality of shade.
V.Suja M.Sc., (Agri.)
UNIT- V 13
Harvest must be done at the time of 50 % of seeds in the umbel is broken, immature and diseased seeds, other crop and weed seeds, mud
exposed, otherwise there will be shattering loss in seed yield. and other inert matters. For processing onion seeds, BSS 10 x 10 wire
mesh sieve should be used.
Methods to improve seed quality
Storage of unprocessed seeds results in poor viability. In processing,
The seeds must be properly separated from the umbels. While seed the sieves must be cleaned while changing to other variety otherwise it
leads to physical admixture results in genetic contamination. Hence,
extraction there should not any damage to seed. Then the seeds must
utmost care must be taken during processing of seeds to maintain
be dried properly. quality.
The properly dried umbels are to be taken in a cloth bag and the Importance must be given for proper storage of seeds until next
seeds may be separated by hand rubbing (or) beating against with season as that of the importance given for production.
pliable bamboo sticks. The extracted seed should be cleaned by
manual winnowing. Moisture content
Seed drying Seed quality mainly depends on the seed moisture. If seed
The extracted seed must be dried properly under shade for 8 to 10 moisture content is high normally it loses its viability at faster rate.
hours after spreading over the gunny bags. Then, it can be dried For short term storage seed moisture content should be reduced to 7-
further under open sun light between 8 to 12 noon and 3 to 5 PM. 8% and can be stored in cloth. For long term storage seed moisture
Avoid drying in between 12 to 3 PM, since the rays emits from sun content should be reduced to 6% and stored in moisture vapor proof
and the heat may affect containers.
the seed viability.
Seed treatment
After extraction seeds should not be dried under direct sun light
between 12-3 pm. And also seeds should not be dried continuously Seeds must be treated with fungicides before storage. The seeds
from the morning to evening. These two factors will affect the may be treated with captan or Thiram @ 4 g/kg of seeds. The seeds
germination. can also be treated with halogen mixture @ 5g/kg of seed and it is a
ecofriendly seed treatment.
Seed processing
Storage containers
After proper drying, the seed processing is essential. This will be
helpful for maintaining high vigour and viability by way of removing Seeds could able to absorb moisture from atmosphere. Hence for
immature and small seeds. In the processing we have to remove storing seeds in the coastal region (or) river sides we should use
V.Suja M.Sc., (Agri.)
UNIT- V 14
moisture vapour proof containers i.e. 700 gauge polyethylene bags. Seed standards
For seed storage every time new containers must be used.
Pure seed (minimum) 98.0%
Seed certification Inert matter (maximum) 2.0%
Other crop seeds
Seed certification guarantees the quality of seed as it ensures that 10/kg
(maximum)
the certified seed has the genetic, physical, physiological and seed Weed seeds (Maximum) 10 /kg
health qualities. Genetic purity means that the seed gives rise to a
Germination (minimum) 70%
plant which conforms to the varietal characteristics of the variety. The
physical purity means that the seed is free from stones, broken seeds, Moisture (maximum)
straw bits and leaf bits etc. Physiological quality is measured by For vapour proof containers 6.0%
germination and seed health envisages freedom from pest and Moisture previous
8.0
diseases. containers
Field standard
Other variety
0.2%
bulbs (max.)
Off types (max.) 0.2%
Irrigation:
At all stages of vegetative growth (55-60 days) and during flower
production sufficient amount of moisture in soil is essential. Moisture
stress at any stage affects normal growth and flowering. In lighter
soils more frequent irrigations are required than in heavy soils. In
sandy loam soil, weekly irrigation is necessary between September to
March while during summer months between April to June irrigation
at 4-5 days intervals is required.
Pinching:
In tall cultivars of African marigold, plants first grow upwards to their
final height and later on produce a terminal flower. After the
formation of terminal flower bud, axillary branches develop which
also bear flower. However, if the apical portion of shoot is removed
early, large numbers of axillary shoots arise resulting in well-shaped
bushy plant bearing more number of uniform sized flowers. Removal
of apical portion of shoot is known as pinching. It is observed that
pinching at 40 days after transplanting enhances flower yield.
However, Giant Double African Yellow and Orange do not require
pinching as the plants are bushy and branching type.
Harvesting:
Marigold flowers are plucked when they have attained full size.
Plucking of flowers should be done in cool hours of the day. The field
should be irrigated before plucking so that flowers keep well for a
longer period after harvest. Plucked flowers are collected in polythene
bags or bamboo baskets for carrying to markets.
After harvesting the flowers they are dried on floor under shade for 2-
3 days. Upon drying, the seeds are separated by hammering the
flowers and the flower straw is separated out by winnowing of the
hammered flowers. The seeds are thoroughly cleaned and graded
before packing in poly bags or cloths bags, and are stored in cool
places. The moisture contents of the seeds must be examined before
the packing.