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Resultados de La Actividad Enzimatica

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8 views1 page

Resultados de La Actividad Enzimatica

Uploaded by

60064538
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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________________________________________________________________________________

The Virtual Laboratory: Enzyme Assay. Copyright © David A Bender, 1982 - 2006
Page 1 of printout for scaef
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1 varying substrate concentration from 0 - 250 mM


100 µL of enzyme no 5
pH = 7
temperature = 30 °C
incubation time = 10 min
substrate µmol product formed (mean of 2 incubations at each point)
mM no inhibitor plus inhibitor A
0.125 0.25 0.5 1 mM

0.0 0.05 0.02 0.02 0.03 0.02


25.0 0.00 0.00 0.00 0.00 0.00
50.0 0.00 0.00 0.00 0.00 0.00
75.0 0.00 0.00 0.00 0.00 0.00
100.0 0.00 0.00 0.00 0.00 0.00
125.0 0.00 0.00 0.00 0.00 0.00
150.0 0.00 0.00 0.00 0.00 0.00
175.0 0.00 0.00 0.00 0.00 0.00
200.0 0.00 0.00 0.00 0.00 0.00
225.0 0.00 0.00 0.00 0.00 0.00
250.0 0.00 0.00 0.00 0.00 0.00
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1 Data for Lineweaver-Burk plot


100 µL of enzyme no 5
pH = 7
temperature = 30 °C
incubation time = 10 min
1/s 1/v (µmol / min) mean of 2 incubations at each point
no inhibitor plus inhibitor A
0.125 0.25 0.5 1 mM

0.0400 173841.7620 167489.7353


170123.2676
173982.8216
170892.5449
0.0200 123689.7759 125617.7959
126782.8199
123840.4720
123820.9969
0.0133 109191.2598 108625.4744
110223.0192
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110506.6687
106823.5378
0.0100 100789.4381 102000.3866
101103.3138
98888.3848102537.2019
0.0080 95470.7537 96159.676694447.587196039.812994109.5709
0.0067 92669.3079 93409.510392792.720992993.030791716.4458
0.0057 90206.5068 90996.663989064.366490357.893290191.3643
0.0050 89607.1015 87670.191989114.774988247.986789667.3423
0.0044 89124.6629 88179.180887780.997585753.079288284.4330
0.0040 87148.5773 85248.047186757.596987906.036687793.1746

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