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Adam Koh ChemPt2-Unit3 Lab#2 Buffers

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47 views9 pages

Adam Koh ChemPt2-Unit3 Lab#2 Buffers

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adamkoh168
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Student Name: _____

Date completed:_____________

Chemistry, Part 2 – FORMAL WRITE UP - Lab Unit THREE – Lab 2

Perform the buffers lab from your lab kit. Complete this formal lab write-up and respond to all
sections in your own words.

Lab Title Buffers

Problem: What are buffers, how do they work, and why are they
What is the problem you are important?
trying to answer? This must be in
question format.

Hypothesis If I set up a buffer solution that tests it, then I can observe how
Write this in an if… then format. they work, and why they are important

Method: First I put 6 drops of water in wells A1-A5 and B1-B5. Then I put a
Briefly describe what you did in mixture of 50 percent sodium acetate and 50 percent acetic acid
the lab (the procedure.) Include into well C1-C5 with a total drop being 6. Then I dropped
chemicals used and any special bromophenol blue into wells A1-A5 and C1-C5 and put in a small
equipment as you describe what strip of phenolphthalein indicator into wells B1-B5 and D1-D5 to
you did.
have a total of 4 different tests of buffer solutions. Lastly I put 2
drops of NaOh in columns A5 down to D5 and one drop from A4
down to D4 then and a drop of HCl from A2 down to D2 and 2
drops from A1 tp D1. I then observed what happened in each well.
Results: 1. The properties of a buffer
1) What type of data did you 2. The results showed that 7 out of 20 wells were not a
collect? buffer solution while the other 13 was, I know this
2)Summarize the chemistry because while all the others had stayed the same color ph
results of the lab. and the original, 7 had either a huge amount of change to
3) What did you observe? Was
their color or a faint change to their color.
there something that you found
3. One thing that I observed was that wells A1 and 2 had
interesting or unusual about the
both changed into yellow which I am curious about how
experiment?
that happened
Discussion 1: A buffer solution is composed of a weak acid and its conjugate
What is the composition of a salt, or a weak base and its conjugate salt
buffer?

Discussion 2: A buffer is a solution that resists changes in pH by neutralizing


What does a buffer do? small amounts of added acid or base. This ability is important for
many chemical reactions and biological processes that require a
stable pH environment.

Discussion 3: A compound that differs by 1 H+


From the prelab video, what is
a conjugate salt? (will not be
accepting a Google answer.)
Discussion 4: Row A because wells A1 and and A2 change from a pH of 7 to
From your lab, which rows had either 3 4
the greatest change in pH and
why?

Conclusion: 1. A buffer is a solution that resists changes in pH when


1) What is the answer to the small amounts of acid or base are added to it and it works
problem question? because a buffer is typically composed of a weak acid and
2)Was your hypothesis correct? its conjugate base or a weak base and its conjugate acid,
Defend your answer with the then when a acid or base is added, the buffer's base or
results of the lab.
acid component neutralizes the added hydrogen ions.
3)What were the sources of
They are important because they maintain a stable pH
error?
environment which is important from a biological
4)What could be done differently
next time in the experiment? standpoint
5)What chemistry did you learn? 2. Yes, my hypothesis was somewhat correct because
although it taught me the properties, it didn't explain why
it was important.
CRAAP Link: https://en.wikipedia.org/wiki/Buffer_solution
Required Photos (6):
1)Add a photo of the wells
after step 3. 2) The second
photo is of the wells after step
4. 3) Add a photo of the wells
after step 5. 4) A photo after
step 6. 5) A photo after step 7.
6) The final photo is of all of
you with the results visible.
Every photo should be labeled
with what is in each row and
column. Be sure that the
labels match what is in each
step, row and column.
Completed Lab Manual Pages:
Insert a photo or scan of your data table 16.1
Completed Lab Manual Questions:

1. a) What was the color of the universal indicator in well D3? Darkish yellow
b) What pH does that indicate? 5
d) What wells have just the buffer? Wells A3,4,5 B1,2,3, C3,4,5, and D1,2,3,4
c) How does that value compare with the theoretical value of 4.8? yes

2. a) What is the pH of pure water? 7


b) Which wells have pure water? (Assume indicators have no effect on the pH.) B1,2,3

3. Use greater than or less than symbols if an exact pH cannot be determined


a) What is the pH of a solution with bromophenol blue that is yellow? < less
b) What is the pH of a solution with bromophenol blue that is purple? > more
c) What is the pH of a solution with phenolphthalein that is clear? < less
d) What is the pH of a solution with phenolphthalein that is pink? > more

4. From row A, how much did the pH of the water change when the HCl was added to columns 1 and 2?
By around 3.5 pH
5. From row C, how much did the pH change when the HCl was added to columns 1 and 2?
Not a lot but it got slight more acidic by like 0.5 ph
6. From row B, how much did the pH of water change when NaOH was added to columns 4 and 5?
Only by a like 1.3 ph because water is 7ph and phenolphthalein detects 8.3+
7. From row D, how much did the pH change when NaOH was added to columns 4 and 5?
By around 2.5 pH
8. Why is aspirin something to be buffered?
So its less irritating int he stomach
9. Do research to find how buffers are used in the human body (acid-base homeostasis). Why are buffers
important for life?
they prevent drastic changes in pH by neutralizing acids or bases such as the bicarbonate buffer system,
the most important in the body, which balances pH in the blood and extracellular fluids

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