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Experiment No. 4

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45 views

Experiment No. 4

Uploaded by

gamingraw20
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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COMPREHENSIVE PRACTICAL CHEMISTRY-X Sirt

56

EXPERIMENT 6.1

Senarate the colourevd components present in the mixture of red and


by ascending paperchromatogruphv and (find their R,values. blue inh
APPARATUS
Gas jar. glass rod, filter paper strip (Whatman filter paper 1), jar cover, fine capillary tuhe
REQUIREMENT
Amixture of red and blue inks, alcohol and distilled water.
PROCEDURE
1. Take a whatman filter paper strip (20 × 2 cm) and draw a line with pencil above 4o
from one end. Draw another line lengthwise from the centre of the
paper as shown in
Fig. 6.4.

Chromatographic
paper

Original line about


4 cm above the edge

Point to put the


spot of the mixture
Fig. 6.4. Spotting of the mixture.
2. With the help of fine capillary tube, put a drop of the
mixture of red and blue inks at
the pointP. Let it dry in air. Put another drop on the same
2-3 times, so that the spot is rich in mixture.
spot and dry again. Repeat
3. Suspend the filter paper vertically in a gas jar
containing the
the help of a glass rod in such a way that the pencil line (and thesolvent (eluent) with
2 cm above the solvent level (50% alcohol + spot)remains about
distilled water).
4) Cover the jar and keep it undisturbed. Notice the
ture of red and blue inks. After the solvent has risen
rising solvent along with the mia
about 15 cm you will notice tWO
different spots of blue and red colours on the filter paper.
5. Take the filter paper out of the jar and mark the
on the paper with a pencil. This is called the
distance that the solvent has rise
solvent front.
6. Dry the paper. Put pencil marks in the
centre of the blue and red spots.
7. Measure the distance of the two spots
from the original line and the distance or
solvent from the original line.
CHROMATOGRAPHY
57

8.
Calculate the R, values of the blue and red inks by using the
formula :
R= Distance travelled by the blue or red ink from the point of applicaton
Distance travelled by the solvent from the original line
OBSERVATIONSANDCALCULATIONS
S. No. Substance Distance travelled Distance travelled R, Value
by different conponents by solvent
1. Red ink + Blue ink A cm (Red Ink) X cm A/X

2. Red ink + Blue ink X cm B/X


Bcm (Blue Ink)

PRECAUTIONS
1. Use good quality pencil for drawing the reference line so that the mark does not
dissolve in the solvent in which the chromatography is carried
2. Always make use of a fine capillary tube.
3. Keep the jar undisturbed and covered during the experiment.
4. A spot should be small and rich in mixture.
5. Allow the spot to dry before putting the strip in the jar.
6. Keep the strip erect. Do not let it to be curled.
7. Do not allow the spot to dip in the solvent.

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