Shaukat Khanum Memorial Cancer Hospital & Research Centre: Serology - Hepatitis
Shaukat Khanum Memorial Cancer Hospital & Research Centre: Serology - Hepatitis
Department of Pathology
VIEW: 08-Aug-2024 16:58:32 Special Chemistry Report Page 1 of 1
COMMENTS:
Anti HCV is a chemiluminescent microparticle immunoassay for the qualitative detection of IgG antibody to hepatitis C virus
in human serum.
This is a screening test.
If there is a non-reactive HCV Antibody result but recent HCV exposure is suspected, than do follow-up testing for HCV
antibody to demonstrate seroconversion (average of 8 to 9 weeks) and test for HCV RNA.
A non-reactive HCV Antibody result does not exclude the possibility of exposure to or infection with HCV.
Non-reactive test results in individuals with prior exposure to HCV may be due to low Antibody levels that are below the
limit of detection of this assay.
A reactive HCV antibody result indicates either current HCV infection, past HCV infection that has resolved or false
reactivity. A reactive result should be followed by HCV RNA testing. If HCV RNA is detected, that indicates current HCV
infection. If HCV RNA is not detected, that indicates either past or resolved HCV infection or false HCV antibody reactivity.
False reactive results can be obtained with heterophilic antibodies in serum, especially in patients routinely exposed to
animals. Also cross-reactivity with other viral infections and diseases is possible leading to false reactive HCV Antibody results.
Reference: Centers for Disease Control and Prevention. Testing for HCV infection: an update of guidance for clinicians and
laboratorians. MMWR Morb Mortal Wkly Rep. 2013 May 10; 62 (18): 362-5.
Please note the change in reference range, effective from 08-11-2023.
METHOD:
Test is performed by chemiluminescent microparticle immunoassay (CMIA) technique.