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Colorful Clean Project Planning Concept Map Graph

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Alimpan
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22 views8 pages

Colorful Clean Project Planning Concept Map Graph

Uploaded by

Alimpan
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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IRON DETECTION

HYDROCHLORIC ACID
(HCL)
IRON STANDARD
SOLUTION
HYDROXYLAMINE
SOLUTION

AMMONIUM ACETATE
REAGENTS
BUFFER SOLUTION

SODIUM ACETATE
SOLUTION

PHENANTHROLINE
SOLUTION
If your sample contains more than 200
TAKE A 50 ML SAMPLE µg of iron, use a smaller portion, dilute
it to 50.0 mL with water, and proceed.

HCL prevents interference of oxidizing


ADD 2ML OF CONC HCL AND 1ML agents
NH2OH.HCL NH2OH.HCL will ensure that all iron is in the
Fe²⁺ state by reducing Fe³⁺ to Fe²⁺.

Heat the mixture until it starts boiling, Continue it


PROCEDURE until the volume reduces to 15-20 mL to ensure all
HEAT THE SOLUTION iron is dissolved.
Add a few glass beads to prevent bumping during
heating

ADD BUFFER Add 10 mL of ammonium acetate (NH₄C₂H₃O₂)


buffer solution to maintain the solution at a pH
SOLUTION suitable for the reaction

Add 4 mL of 1,10-phenanthroline solution to form a


colored complex with Fe²⁺
Dilute the solution to the 100 mL mark with
COLOUR distilled water and mix thoroughly and stand for at
DEVELOPMENT least 10 minutes to allow maximum color
development (the solution will turn orange-red).
STANDARD Create a range of 10 standard solutions
containing 1 to 100 µg of iron in a final volume

SOLUTION of 100 mL.


Dilute the solutions.

Add 2 mL of concentrated HCl , 1 mL of


NH₂OH·HCl solution, 10 mL of ammonium
ADD REAGENTS acetate buffer and 4 mL of 1,10-phenanthroline
solution to each standard solution as did with
sample.

Set the instrument to a wavelength of

SPHECTROPHOTOMETER SETUP INSTRUMENT 510 nm.


Use distilled water as the reference to
set zero absorbance.

Measure the absorbance of each of the 10


MEASURE THE iron standards.
Record the values and plot a calibration
STANDARDS curve of absorbance vs. iron concentration.

Measure the absorbance of the sample


MEASURE THE solution.
Find the concentration of iron by
SAMPLE comparing the sample’s absorbance to the
calibration curve.
MANGANESE DETECTION
75G OF HGSO4 IN 400ML OF HNO3 AND
SPECIAL REAGENT 200ML OF DISTILLED WATER.ADD 200ML OF
H3PO4 AND 35 MG OF AGNO3.DILUTE TO 1 L

AMMONIUM PERSULPHATE
(NH4)2S2O8

DISSOLVE 1G OF MANGANESE
STANDARD MANGANESE METAL IN 10 ML
REAGENTS SOLUTION HN03.DILUTE TO 1000 ML
WITH 1% (V/V) HCL

HYDROGEN PEROXIDE,NITRIC
ACID,SULPHURIC ACID
PROCEDURE

TAKE A SUITABLE PORTION DILUTE TO 90ML WITH


ADD 5ML SPECIAL REAGENT AND 1 DROP H2O2
OF SAMPLE DISTILLED WATER

ADD (NH4)2S2O8 AND BOIL


FOR 1 MIN.THEN COOL DILUTE TO 100 ML
UNDER TAP.

PREPARE STANDARD
SOLUTION FROM
0,5,10...1500 MICROGRAMS
STANDARD Create a range of solutions from 0 to 1500

SOLUTION
micrograms per 100 ml of final solution

Make photometric measurements using

SETUP INSTRUMENT spectrophotometer

Set the instrument to a wavelength of 520 nm.

SPHECTROPHOTOMETER REFERENCE Use distilled water as the reference to


set zero absorbance.

Measure the absorbance of each of the


MEASURE THE samples present
Record the values and plot a calibration
STANDARDS curve of absorbance vs. mangnese
concentration.

Measure the absorbance of the sample


MEASURE THE solution.
Find the concentration of iron by
SAMPLE comparing the sample’s absorbance to the
calibration curve.

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