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TP Hema

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Micah Malubag
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23 views1 page

TP Hema

Uploaded by

Micah Malubag
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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TECHNICAL PROCEDURES IN PERFORMING LABORATORY TESTS: HEMATOLOGY

1) Complete Blood Count

 Specimen: Whole Blood


 Check if the information in the test tube label matches with that of the requisition form.
 Invert the tube 8-10 times.
 Ensure the analyzer is turned on.
 Remove the tube stopper, insert the sample tube in the sample probe.
 Press the button behind the probe, and allow the probe to aspirate sample.
 Remove the sample tube as soon as you hear a beep sound, this indicates that enough sample has
been aspirated.
 Wait until the results are flashed on the screen.
 Print out results.

2) Clotting time (Slide method)

 Sample: Fresh capillary blood


 Collect a small drop of capillary blood in the capillary tube.
 Gently expel blood onto the glass slide, forming a small drop.
 Tilt the slide back and forth, then rotate it gently to spread the blood evenly.
 Observe the blood drop for clot every 30 seconds.
 Record the time it took for the blood to form fibrin clots.

3) Bleeding time

 Sample: Fresh capillary blood


 Ensure the patient has not taken medications affecting bleeding time.
 Make a small prick on the selected site using the sterile lancet.
 Allow blood drop to form on the site.
 Wipe the blood drop on the site every 30 seconds, until the bleeding stops.
 Record the time it took for the bleeding to stop.

4) ABO and Rh Typing (Forward Typing)

 Mark a clean glass-slide as anti-A, anti-B, and anti-D.


 Place 1 drop of blood on each marked space.
 Add 1 drop of anti-A serum to the blood on the space marked with anti-A.
 Add 1 drop of anti-B serum to the blood on the space marked with anti-B.
 Add 1 drop of anti-D serum to the blood on the space marked with anti-D.
 Using an applicator stick, mix each side of the reaction.
 Rock the slide back and forth and observed for agglutination.
 Record the observation.
 Interpretation of results:
o Agglutination with anti-A: Blood Group A
o Agglutination with anti-B: Blood Group B
o Agglutination with both anti-A and anti-B: Blood Group AB
o No agglutination with both anti-A and anti-B: Blood Group O
o Agglutination with anti-D: Rh positive
o No agglutination with anti-D: Rh negative

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