DR /4000 UV - VIS

Spectrophotometer

The totally integrated, complete laboratory testing solution

that makes analysis easy, accurate and more
convenient than ever before.
DR /4000 UV - VIS
Spectrophotometer

The DR/4000 Spectrophotometer is a powerful scanning

spectrophotometer that gives you advanced capabilities
and performance over any other instrument in its class.
• Expand your laboratory’s testing abilities using the ultraviolet (UV) testing
capabilities for applications and measurements requiring wavelengths not
available on visible-light-only spectrophotometers.

• Speed analysis of a wide range of samples and enable your analysts to carry
out unique experiments using preprogrammed procedures and storage capacity
for custom calibrations.

• Study kinetics using time-course measurement or complete other research

projects using its ability to automatically complete multiple scans.

The DR/4000 is ideal for analyzing compounds that require multiple wave-
lengths, longer path lengths or time course operations. It is available in
two models:

• DR/4000 U has ultraviolet and visible spectrum analysis capabilities with a

range of 190 to 1100 nm.

• DR/4000 V has visible spectrum capabilities with a range of 320 to 1100 nm.

2
E x p a n d
Your Capabilities
The DR/4000 is ideally suited for applications in diverse fields
such as water and wastewater treatment, industrial processes,
quality control, food and beverage processing, pharmaceutical
production and chemical synthesis. And because the DR/4000
Spectrophotometer is part of a complete solution that supplies
everything you need to run even the most complicated tests,
you can expand your testing capabilities. You get:

• An intuitive user interface to simplify the

most complex methods.

• 130 Preprogrammed Hach methods, which

are proven procedures based on EPA accepted
standard methods.

• Illustrated manuals – one for operating the

instrument plus another for running the tests.

• The best personal service and technical

support right from Hach, as close as
your telephone.

While other spectrophotometers may claim they are easy to

use, none can offer the total integration of reagents,
methods, reference manuals and service that distinguishes a
Hach system from the others.

Switch Modules in Seconds

The patented modular sample compartment
accommodates a variety of innovative
accessories. Modules and cell holders swap
out in seconds without having to turn off
the DR/4000. See page 12 for more
information about module configurations.

3
 The D R
/4 0 0 0
Surpasses Comparable
Spectrophotometers
Designed to increase Resolution and Accuracy
productivity and The DR/4000 gives you industry-leading accuracy and outstanding precision
for the most reliable test results. You can set the wavelength step size as
efficiency without small as 0.1 nm, and the sample blank correction feature permits automated
measurements with precision and stability. And when you power up the
sacrificing accuracy, DR/4000, it automatically aligns the lamp, performs a wavelength calibration
and an offset correction; and performs memory, voltage and system tests.
the DR/4000 outper-
forms any other Scanning Measurements
spectrophotometer The DR/4000 U can scan across the range of 190 to 1100 nm, while the
DR/4000 V scans from 320 to 1100 nm. With the scan overlay, scan add and
in its class. Take a scan subtract features, you can easily analyze and compare sample results.
The DR/4000 U automatically performs lamp changeover when it moves
closer look at its from visible to UV wavelengths.

advanced features – Ergonomic Graphic Display

all included with the The large, liquid crystal display is backlit to reduce eyestrain and fatigue, and
instrument unless brightness and viewing angle can be adjusted according to your preferences.
During instrument operation, the display shows you a graphical
otherwise noted. representation of the calibration curve and data points along with
quantitative data generated during the actual analysis.

4
Flexible Sample Handling
The innovative, patented modular sample
compartment can accommodate multi- or single-
cell holders. In addition to handling one-inch,
one-centimeter, five-centimeter and 10-centimeter
sample cells, the DR/4000 also accommodates:

• four-, five- and six-position carousels

• microcell adapters
• 16-millimeter (COD, Test ‘N Tube™, and UniCell™)
vial adapters
• AccuVac® Ampul adapters
• Temperature control module

Sipper Modules and Flow-Cell Modules can also

be used for increasing sample throughput.

Data Storage and Recall

Store, recall or print data with the push of a
button. Store and recall up to 200 data points,
10 spectral scans and 10 time course scans
automatically or manually. Print data or send
it to a computer at the time of measurement
or upon recall by simply pressing the print key.

Exceptional Stability and a

Space-Saving Design
The DR/4000 Spectrophotometer’s optical design
provides an exceptionally stable analysis system
in a small, efficient package. The DR/4000 goes
through an internal, self-test procedure upon
power-up with visual feedback on the display. HachLink™ Software* Ensures
Data Integrity
Computer Interface
Using the intuitive HachLink Software will
Because results documentation is an integral help your program meet GLP requirements for
component of the analysis process, the DR/4000 data collection. This Microsoft® Windows®-
is equipped to download data to a printer, PC compatible software program, available as an
or LIMS. Data can be output as a text file option, enables your personal computer to
for spreadsheet or word processing capture fresh or stored data from the DR/4000
software applications. and transfer it to text files or to Microsoft® Excel.
The software automatically records instrument,
test and sample data at the push
Multi-Language Capability of a button.
Display messages guide you through proper key HachLink system requirements:
sequences for each testing and programming • IBM PC/AT or compatible with a 486/100 mHz
procedure. The system comes pre-installed with six or higher processor
languages: English, French, Spanish, German, • 3MB of RAM
Portuguese and Japanese. • Hard-disk drive with at least 20MB of free space
• 3.5" 1.44 MB floppy disk drive or CD-ROM drive
• VGA graphics with 640 x 480 or higher
resolution, 256 colors
• Mouse or other pointing device
• 9-pin serial port or 25-pin serial port with
9 pin adapter
• Windows® 95/98/2000 or NT 4.0
• Computer interface cable

*Not included

5
 Advanced
Guides You Through Each Test
Software
Conduct Sophisticated Experiments
The DR/4000’s advanced software enables you to conduct experiments on
challenging samples. Most operations can be accomplished with just a few
keystrokes, and intuitive messages guide you from one screen to the next.

Here’s an illustration of how to study a new analyte using a phosphorus

test as an example.

Scanning Function
First, identify the optimum wavelength at which the
study should be conducted by scanning across the
wavelength range. Just select the wavelength scan range,
key in the rate at which the data will be collected, and
select either %T or ABS. You will then be prompted by
the instrument to take a baseline and start the data
collection. The data is available either tabulated or
displayed in a graphical form, with peaks and valleys
located automatically. Zoom in or out to view a selected
range of data. Automatically or manually store the scan
for future recall. Scans may be overlayed, and then
added or subtracted.

Time-Course Function
In the next phase of the experiment, you may wish to
ascertain the length of time required for maximum color
development in the test sample. With a DR/4000, you
can monitor changes in %T or ABS at a single
wavelength over a defined period of time. Just select the
wavelength, key in the time period (hours, minutes or
seconds) and select either %T or ABS. The instrument
will store the data, which can be recalled onto the
screen in graphical or tabular form for further study.

6
 User Calibration
In the final phase of the study, you must generate a calibration
curve. After selecting the option for user-entered calibrations,
you are prompted to enter the appropriate information into a
table. As the measurements are made, the DR/4000 prompts
you for each standard sample. Once the data collection is
completed, the DR/4000 prompts you to select linear,
quadratic, cubic or piece-wise linear formulas. The calibration
curve is displayed in graphical or tabular format for review. If
desired, you can add more points or delete outliers. You can
also identify alternative chemical species (P instead of
PO4-3 for example) at this time, if desired.

Custom Calibrations
Store up to 200 of your own calibrations in its memory. Each
calibration curve can contain up to 16 data points. A simple
programming procedure makes it easy to key in your data to
adapt the instrument to your laboratory’s protocols or special
testing requirements. The DR/4000 accommodates any test
within its analysis range. Just establish a calibration curve
using the appropriate data or standards, and store.

Preprogrammed Calibrations
More than 130 preprogrammed Hach methods are permanently
stored in the instrument’s non-volatile memory. You can call up
wavelength measurements with just a few keystrokes, and a
user-oriented interface guides you through each procedure.
As new methods become available, they are packaged into
software upgrades you can purchase to update your DR/4000.

Standard Additions
To ensure confidence in your results, the DR/4000 includes a standard
additions option. Just key in the appropriate information, add an aliquot
of standard to your sample and the DR/4000 calculates the percent
recovery. Used with Hach calibrations or user-entered methods, standard
additions can identify interferences, bad reagents or faulty procedures.

Multiwavelength Analysis
With the DR/4000, you can measure a sample at up to four different
wavelengths, in rapid succession, in one operation.

7
 Sophisticated Optics
The DR/4000’s optical system consists of a Seya-
Namioka split-beam diffraction grating monochroma-
tor, a light source and silicon photodiode detectors.
Innovative reference beam technology ensures stable,
accurate readings. A halogen gas-filled tungsten lamp
is the light source for visible spectrum analysis, while
a deuterium lamp provides the light source for UV When the light source is activated, the optics system
separates the light into a monochromatic beam, which
analysis. When you power up the DR/4000, a self-test passes through the sample cell to the photodetector.
procedure checks offset correction, wavelength calibra- The photodetector responds to the light’s intensity and
relays that information to the microprocessor. Readout
tion, lamp alignment, memory system and voltages. displays in concentration, ABS or %T.

J
F
E
G
A
H
I B

K C
I

D B

Deuterium Lamp A
Aperture Stop B
Rotating Concave Spherical Mirror C
Tungsten Halogen Lamp D
Rotating Concave Holographic Grating E
Order Sorting Filter F
Reference Detector G
Fused Silica Beam Splitter H
Convex Fused Silica Lens I
Sample Cell J
Measurement Detector K

8
Sample Specification
for
DR/4000 UV-VIS
The spectrophotometer is a scanning, laboratory instrument
capable of analysis in the near infrared, visible and ultraviolet
spectrum. It has an intuitive user interface to simplify complex
methods; proven, preprogrammed procedures based on accepted
standard methods; illustrated manuals for both operating the
instrument and running the tests; and technical support available
by telephone and/or web site.

A backlit, liquid crystal display screen is provided for the graphical display of
spectral scans, calibration curves and data points along with quantitative data
generated during the analysis. Display contrast and viewing angle is adjustable.
Operator-selectable readout modes is available for concentration, ABS and %T.
Alpha-numeric prompting messages and menus is provided on the display in multiple
languages including English, French, Spanish, German, Portuguese and Japanese.

The spectrophotometer is capable of automated scanning, multiple wavelength and

time course operations and accommodates a variety of sample cells including 1-inch,
1-cm, 5-cm, 10-cm, 16-mm and microcells. A standard additions option is available
to identify interferences, bad reagents or faulty procedures. The instrument has the
ability to measure a sample at up to four different wavelengths in rapid succession
in one operation. Scan overlay, scan add and scan subtract features are available to
enable users to easily analyze and compare sample results.

The spectrophotometer has a patented quick change, plug-in modular sample

compartment that accommodates Single Cell Modules, Carousel Modules, Sipper
Modules, Flow-Cell Modules and Temperature Control Modules. Modules and cell
holders swap out easily without having to power down the unit.

The optical system is composed of a Seya-Namioka split-beam monochromator,

tungsten halogen (visible spectrum) and deuterium (UV spectrum) light sources, and
silicon photodiode detectors. The monochromator provides a full 190 to 1100-nm
wave-length range with a nominal spectral bandwidth of 4 nm. Wavelength step size
has the ability to be set as small as 0.1 nm. A sample blank correction feature permits
precise and stable automated measurements. The spectrophotometer provides
automated changeover between visible and ultraviolet source lamps. Wavelength
accuracy is ±1 nm. The spectrophotometer carries out an automated self-test at
power-up, including wavelength calibration, automatic lamp alignment, offset
correction, and memory, voltage and system tests.

A read-only memory (ROM) is provided with preprogrammed calibration data for more
than 130 colorimetric test procedures to provide direct readout in concentration mode
without the need for calculations or calibration curves. The instrument provides
storage of up to 200 user-generated calibrations with linear, cubic, quadratic and
piecewise curve-fitting capability. It has the capacity to store up to 200 data points,
10 spectral scans, and 10 time course scans, and is able to store, recall or print data
with the push of a button. Users have the ability to download data to a printer, PC
or LIMS, and, using Microsoft® Windows®-compatible software, output it as a text file
or spreadsheet.

The spectrophotometer is constructed of impact-resistant structural plastic and carries

either the ETL certification mark for UL and CSA safety standards (AC line power
is auto selectable for 95 to 240 Vac, 50/60Hz) or the European Union (CE)
certification mark (European model).

9
 Parameters, Methods,
The table below lists test ranges, methods of analysis and corresponding reagent sets for the DR/4000
Spectrophotometer. The complete procedure for each test is included in the manual supplied with the instrument.
The ranges are given for the precalibrated instrument readout; higher ranges can be analyzed by sample dilution.

Test Method DR/4000 Range EDL*** Cat. No.

Aluminum Aluminon 0-0.800 mg/L 0.005 22420-00
Aluminum Eriochrome Cyanine R 0-0.250 mg/L 0.002 26037-00
Aluminum, UniCell™ Chromazurol S 0.02 - 0.50 0.02 HCT 150†††
Ammonia, Free (Monochloramine and Nitrogen) Indophenol 0.02-0.50 mg/L -- 28797-04
Ammonium, UniCell™ Indophenol 0.05-1.50 mg/L 0.05 HTC 100
Ammonium, UniCell™ Indophenol 1.50-45.0 mg/L 1.5 HTC 102
Arsenic (USEPA) Silver Diethyldithiocarbamate 0-0.200 mg/L NA --
Barium Turbidimetric 0-100 mg/L NA 12064-66
Barium (AccuVac®) Turbidimetric 0-100 mg/L NA 25130-25
Benzotriazole UV Photolysis 0-16.0 mg/L 0.3 21412-99
Boron Carmine 0-14.0 mg/L 0.4 14170-99
Boron, LR Azomethine-H 0-1.50 mg/L 0.02 26669-00
Bromine DPD 0-4.50 mg/L 0.03 21056-69
Bromine (AccuVac®) DPD 0-4.50 mg/L 0.02 25030-25
Cadmium Dithizone 0-80 µg/L 1.3 µg/L 22422-00
Cadmium, UniCell™ Cadion/CN- 0.02 - 0.30 0.02 HCT 154†††
Chloramine, mono, LR Indophenol 0-4.50 mg/L 0.09 28022-46
Chloramine, mono, HR Indophenol 0-10.0 mg/L 0.1 28051-45
Chloride Mercuric Thiocyanate 0-25.00 mg/L 0.24 23198-00
Chlorine, free (USEPA) DPD 0-2.00 mg/L 0.01 21055-69
Chlorine, free (AccuVac®) (USEPA) DPD 0-2.00 mg/L 0.01 25020-25
Chlorine, free, Test ‘N Tube™ DPD 0-5.00 mg/L 0.04 21055-45
Chlorine, free, HR DPD 0-5.00 mg/L 0.02 14070-99
Chlorine, total (USEPA) DPD 0-2.00 mg/L 0.01 21056-69
Chlorine, total (AccuVac®) (USEPA) DPD 0-2.00 mg/L 0.01 25030-25
Chlorine, total, Test ‘N Tube™ DPD 0-5.00mg/L 0.04 21056-45
Chlorine, total, ULR (USEPA) DPD 0-500 µg/L 3 µg/L 25630-00
Chlorine, total, HR DPD 0-5.00 mg/L 0.02 14064-99
Chlorine dioxide DPD 0-5.00 mg/L 0.04 27709-00
Chlorine dioxide (AccuVac®) DPD 0-5.00 mg/L 0.04 27710-00
Chlorine dioxide, LR Chlorophenol Red 0-1.00 mg/L 0.02 22423-00
Chlorine dioxide, MR Direct Reading 0-50 mg/L 0.6 --
Chlorine dioxide, HR Direct Reading 0-1000 mg/L 2 --
Chlorophyll-a Acetone Extraction -- -- 27480-00
Chromium, hexavalent (USEPA) 1,5-Diphenylcarbohydrazide 0-0.700 mg/L 0.006 12710-99
Chromium, hexavalent (AccuVac®) (USEPA) 1,5-Diphenylcarbohydrazide 0-0.700 mg/L 0.005 25050-25
Chromium, hexavalent (for soil) 1,5-Diphenylcarbohydrazide 0-0.700 mg/L 0.006 12710-99
24497-99
Chromium, total Alkaline Hypobromite Oxidation 0-0.700 mg/L 0.003 22425-00
Chromium, UniCell™ H3PO4, Peroxodisulfate, Diphenylcarbazide 0.03 - 1.00 0.3 HCT 156†††
Chromium, trivalent Colorimetric 0-20.0 g/L 0.1 g/L --
Cobalt PAN 0-2.00 mg/L 0.01 26516-00
Color ADMI 0-250 units Pt-Co 3 ADMI --
Color, Gardner ASTM D 6166-97 1-18 units -- --
Color, True and Apparent Platinum-Cobalt 0-500 units 2 units Pt-Co --
Color, Tristimulus and Chromaticity ASTM E 308-95 -- -- --
Color, Yellowness Index ASTM E 313-96 -- -- --
Copper (USEPA) Bicinchoninate 0-5.000 mg/L 0.021 21058-69†
Copper (AccuVac®) (USEPA) Bicinchoninate 0-5.000 mg/L 0.020 25040-25†
Copper Porphyrin 0-210.0 µg/L 1.4 µg/L 26033-00
Copper, autocatalytic Colorimetric 0-3.00 g/L -- 1042-66
Copper, UniCell™ Bathocuproin 0.1 - 6.00 0.1 HCT 163†††
Cyanide Pyridine-Pyrazalone 0-0.240 mg/L 0.0003 24302-00
Detergents, anionic Crystal Violet 0-0.275 mg/L 0.005 24468-00
Fluoride (Reagent Solution) (USEPA) SPADNS 0-2.00 mg/L 0.02 444-49††
Fluoride (AccuVac®) (USEPA) SPADNS 0-2.00 mg/L 0.04 25060-25††
Fluoride, UniCell™ SPADNS 0.1 - 1.5 0.1 HCT 132
Formaldehyde MBTH 0-500 µg/L 6 µg/L 22577-00
Hardness, total, ULR Chlorophosphonazo 0-1000 µg/L 4 µg/L 26031-00
Hardness, calcium and magnesium as CaCO3 Calmagite Colorimetric 0-4.00 mg/L 0.03 23199-00
Hydrazine p-Dimethylaminobenzaldehyde 0-600 µg/L 2.4 µg/L 1790-32
Hydrazine (AccuVac®) p-Dimethylaminobenzaldehyde 0-600 µg/L 4.3 µg/L 25240-25
Iodine DPD 0-7.00 mg/L 0.04 21056-69
Iodine (AccuVac®) DPD 0-7.00 mg/L 0.04 25030-25
Iron FerroZine® 0-1.400 mg/L 0.004 2301-66
Iron, ferrous 1,10-Phenanthroline 0-3.000mg/L 0.008 1037-69
Iron, ferrous (AccuVac®) 1,10-Phenanthroline 0-3.000 mg/L 0.007 25140-25
Iron, total (USEPA) FerroVer® 0-3.000 mg/L 0.008 21057-69†
Iron, total (AccuVac®) (USEPA) FerroVer® 0-3.000 mg/L 0.007 25070-25†
Iron, total FerroMo™ 0-1.800 mg/L 0.025 25448-00
Iron, total TPTZ 0-1.800 mg/L 0.022 26087-99
Iron, total (AccuVac®) TPTZ 0-1.800 mg/L 0.008 25100-25
Iron, UniCell™ Phenanthroline 0.1 - 5.00 0.1 HCT 159†††
Lead (USEPA) Dithizone 0-300 µg/L 3 µg/L 22431-00†
Lead (LeadTrak™) Fast Column Extraction 0-150 µg/L 2 µg/L 23750-00
Lead, UniCell™ PAR/CN- 0.1 - 2.00 0.1 HCT 152†††
Manganese, LR PAN Method 0-0.700 mg/L 0.005 26517-00
Manganese, HR (USEPA) Periodate Oxidation 0-20.0 mg/L 0.1 24300-00†
Mercury Cold Vapor 0.1-2.5 µg/L 0.1 µg/L 26583-00
Metals Prep Set, UniCell™ -- -- -- HCT 200†††
Molybdenum, Molybdate, LR Ternary Complex 0-3.00 mg/L 0.03 24494-00
Molybdenum, Molybdate, HR Mercaptoacetic Acid 0-50.0 mg/L 0.1 26041-00
Molybdenum, Molybdate, HR (AccuVac®) Mercaptoacetic Acid 0-50.0 mg/L 0.1 25220-25
Monochloramine Salicylate 0-0.50 mg/L 0.003 26184-00
Monochloramine (AccuVac®) Salicylate 0-0.50 mg/L 0.003 25210-98
Monochloramine (Free Ammonia and Nitrogen) Indophenol 0.02-0.50 mg/L -- 28797-04
Nickel (USEPA) Heptoxime 0-1.80 mg/L 0.01 22435-00†
6
Reagent Sets and Ranges
Parameters marked (USEPA) are USEPA-approved or accepted for reporting purposes; sample pretreatment may be required on some procedures.
If no reagent set is listed for a parameter, order needed reagents and supplies separately.

Test Method DR/4000 Range EDL*** Cat. No.

Nickel PAN 0-1.000 mg/L 0.005 22426-00
Nickel, autocatalytic Photometric 0-8.00 g/L 0.05 g/L 14321-98
Nickel, UniCell™ Dimethylglyoxime 0.1 - 6.00 0.1 HCT 167†††
Nitrate, UniCell™ Dimethylphenol 1.0-60.0 mg/L 1.0 HCT 106
Nitrogen, ammonia (USEPA) Nessler 0-2.500 mg/L 0.017 24582-00††
Nitrogen, ammonia Salicylate 0-0.80 mg/L 0.09 22437-00
Nitrogen, ammonia LR Test ‘N Tube™ Salicylate 0-2.500 mg/L 0.031 26045-45
Nitrogen, ammonia HR Test ‘N Tube™ Salicylate 0-50.0 mg/L 0.6 26069-45
Nitrogen, Monochloramine and Free Ammonia Indophenol 0.02-0.50 mg/L -- 28797-04
Nitrogen, Nitrate UV Direct Reading 0-10.2 mg/L 0.2 23213-53
Nitrogen, Nitrate, LR Cadmium Reduction 0-0.50 mg/L 0.01 24298-00
Nitrogen, Nitrate, MR Cadmium Reduction 0-5.0 mg/L 0.1 21061-69
Nitrogen, Nitrate, MR (AccuVac®) Cadmium Reduction 0-5.0 mg/L 0.1 25110-25
Nitrogen, Nitrate, HR Cadmium Reduction 0-30.0 mg/L 0.5 21061-69
Nitrogen, Nitrate, HR (AccuVac®) Cadmium Reduction 0-30.0 mg/L 0.3 25110-25
Nitrogen, Nitrate HR Test ‘N TubeTM Chromotropic Acid 0-30.0 mg/L 0.2 26053-45
Nitrogen, Nitrite, LR (USEPA) Diazotization 0-0.3000 mg/L 0.0008 21071-69
Nitrogen, LR (AccuVac®) (USEPA) Diazotization 0-0.3000 mg/L 0.004 25120-25
Nitrogen, Nitrite, LR Test ‘N TubeTM Diazotization 0-0.5000 mg/L 0.0013 26083-45
Nitrogen, Nitrite, HR Ferrous Sulfate 0-250 mg/L 1 21075-69
Nitrogen, total, UniCell™ Alkaline Digest/Dimethylphenol 5.0-40.0 mg/L 5 HCT 111*
Nitrogen, total Inorganic Test ‘N TubeTM Titanium Trichloride Reduction 0-25.0 mg/L 0.3 26049-45
26045-45
Nitrogen, total Test ‘N TubeTM Persulfate Digestion 0-25 mg/L 2 26722-45*
Nitrogen, total, HR Test ‘N TubeTM Persulfate Digestion 10-150 mg/L 7 27141-00*
Nitrogen, total Kjeldahl Nessler 0-150 mg/L 1.2 24953-00†
Organics Constituents, UV Absorbing Direct -- -- 26415-53
Oxygen demand, chemical, ULR Reactor Digestion 0-40 mg/L 0.2 24158-25*
Oxygen demand, chemical (USEPA) Reactor Digestion 0-150 mg/L 1.1 21258-25*
Oxygen demand, chemical, HR (USEPA) Reactor Digestion 0-1500 mg/L 3 21259-25*
Oxygen demand, chemical, HR Plus Reactor Digestion 0-15,000 mg/L 30 24159-25*
Oxygen demand, chemical Manganese III Digestion 20-1000 mg/L 4 26234-25*
Oxygen, dissolved, LR (AccuVac®) Indigo Carmine 0-1000 µg/L 10 µg/L 25010-25
Oxygen, dissolved, HR (AccuVac®) HRDO 0-15.0 mg/L 0.1 25150-25
Oxygen, dissolved, Super HR (AccuVac®) SHRDO 0-40.0 mg/L 0.2 25150-25
Oxygen Scavengers (DEHA) Iron Reduction 0-500 µg/L 5 µg/L 24466-00
Ozone, LR (AccuVac®) Indigo 0-0.25 mg/L 0.01 25160-25
Ozone, MR (AccuVac®) Indigo 0-0.75 mg/L 0.01 25170-25
Ozone, HR (AccuVac®) Indigo 0-1.50 mg/L 0.008 25180-25
Palladium N,N’-Dimethyldithiooxamide 0-250 mg/L -- 23123-00
PCB (in soil) Immunoassay -- -- 25859-00**
Phenols (USEPA) 4-Aminoantipyrine 0-0.200 mg/L 0.001 22439-00††
Phosphate, UniCell™ Digestion - Molybdate/Ascorbic Acid 1.5-15.0 mg/L 1.5 HCT121*
Phosphate, UniCell™ Digestion - Molybdate/Ascorbic Acid 6.0-60.0 mg/L 6 HCT122*
Phosphonates Persulfate/UV Oxidation 0-2.50 to 0-125 mg/L 0.045 -2.25 24297-00
Phosphorus, acid hydrolyzable, Test ‘N TubeTM Ascorbic Acid with Acid Hydrolysis 0-5.00 mg/L 0.17 27427-00*
Phosphorus, reactive Molybdovanadate 0-45.0 mg/L 0.09 20760-32
Phosphorus, reactive (AccuVac®) Molybdovanadate 0-45.0 mg/L 0.24 25250-25
Phosphorus, reactive, LR Test ‘N TubeTM (USEPA) Ascorbic Acid 0-5.00 mg/L 0.02 27425-45
Phosphorus, reactive (USEPA) Ascorbic Acid 0-2.500 mg/L 0.045 21060-69
Phosphorus, reactive (AccuVac®) (USEPA) Ascorbic Acid 0-2.500 mg/L 0.031 25080-25
Phosphorus, reactive Amino Acid 0-30.00 mg/L 0.04 22441-00
Phosphorus, reactive, HR Test ‘N TubeTM Molybdovanadate 0-100.0 mg/L 5.0 27673-45
Phosphorus, total, digestion (USEPA) Acid Persulfate Digestion -- -- 2451-99
Phosphorus, total, LR Test ‘N TubeTM (USEPA) Ascorbic Acid with Acid Persulfate Digestion 0-3.50 mg/L 0.06 27426-45*
Phosphorus, total, HR Test ‘N TubeTM Molybdovanadate with Acid Persulfate Digestion 0-100.0 mg/L 5.0 27672-45*
Platinum N,N’-Dimethyldithiooxamide 0-10 g/L -- 24596-00
Polyacrylic Acid Absorption - Colorimetric 0-20.0 mg/L 0.2 - 0.3 22252-00
Potassium Tetraphenylborate 0-7.0 mg/L -- 24591-00
Quaternary Ammonium Compounds Direct Binary Complex 0-5.00 mg/L 0.11 24592-00
Selenium Diaminobenzidine 0-1.000 mg/L 0.003 22442-00
Silica, HR Silicomolybdate 0-100.0 mg/L 0.3 24296-00
Silica, LR Heteropoly Blue 0-1.600 mg/L 0.01 24593-00
Silica, ULR Heteropoly Blue 0-1000.0 µg/L 1.0 µg/L 25535-00
Silver Colorimetric 0-0.700 mg/L 0.006 22966-00
Sulfate (USEPA) SulfaVer® 4 0-70.0 mg/L -- 12065-99
Sulfate (AccuVac®) (USEPA) SulfaVer® 4 0-70.0 mg/L -- 25090-25
Sulfate, UniCell™ Barium Sulfate Turbidity 40-150 mg/L 40 HTC 125
Sulfate, UniCell™ Barium Sulfate Turbidity 150-900 mg/L 150 HTC 126
Sulfide (USEPA) Methylene Blue 0-800 µg/L 2.4 µg/L 22445-00
Surfactants, Anionic - see Detergents
Tannin and Lignin Tyrosine 0-9.00 mg/L 0.09 22446-00
Tolyltriazole UV Photolysis 0-16.0 mg/L 0.4 21412-99
Total Organic Carbon, HR Direct 100-700 mg/L 4 27604-45*
Total Organic Carbon, MR Direct 15-150 mg/L -- 28159-45*
Total Organic Carbon, LR Direct 0-20.0 mg/L 0.4 27603-45*
Trihalomethanes THM Plus 6-200 ppb 6 ppb 27908-00
Toxicity ToxTrak™ 0-100% I -- 25972-00
TPH (in soil) Immunoassay 10 and 100 ppm TPH -- 26026-00**
TPH (in water) Immunoassay - -- 26237-00**
Turbidity Radiation Attenuation 0-5000 FAU 14 FAU -
Volatile Acid Esterification 0-2800 mg/L 12 22447-00
Zinc (USEPA) Zincon 0-3.000 mg/L 0.009 24293-00†
Metals Prep Set -- -- -- HTC 200
ULR indicates ultra-low range, LR indicates low range, †Method requires digestion. *Vial test requires digestion; Order the DRB 100 Reactor or COD Reactor separately.
MR indicates mid range, and HR indicates high range. ††Method requires distillation. **Additional apparatus required: Microcell adapter, 1-cm (48588-00); immunoassay sample cell (26295-00).
††† UniCell™ metals tests require use of the Metals Prep Set HCT ***Estimated Detection Limit, mg/L, unless stated otherwise.
200 and digestion with DRB 100 Reactor or COD Reactor, all
ordered separately, for "total" metal reading.
 Customize your
DR /4000 with
System Accessories
The DR/4000 V includes these
accessories as standard equipment:
• Single Cell Module
• Pair of 1-inch matched sample cells
• 1-cm cell adapter
• AccuVac® and 16-mm vial adapters
• 1-inch cell adapter
• Illustrated manual set on CD-ROM
• Dust cover
• Replacement tungsten lamp kit
• Power cord

The DR/4000 U includes the above, plus:

• Carousel Module with 1-inch, four-
position and 1-cm, six-position
carousel inserts
Adapters and Sample Cells
• Pair of 1-cm quartz sample cells A wide choice of adapters and sample cells are
available for the DR/4000 to increase your testing
To increase speed and convenience of your flexibility. Select from:
analyses, optional accessories for the • Six-, five- and four-position cell adapters for
DR/4000 are also available: multiple sample testing with the Carousel Module.
• 50-mm and 100-mm longpath cells and adapters
for increased sensitivity.
Adapters and Sample Cells • A variety of glass, quartz and plastic disposable
sample cells.
Sipper System • Disposable microcells and a microcell adapter for
immunoassay or low volume applications.
COD/Test ‘N Tube™/UniCell™
Carousel Adapter Sipper System
Flow-Cell Module The Sipper Module automatically handles small-
volume sampling tasks while eliminating cell
Temperature handling or breakage. In just a few seconds, the
Control Module Sipper Module’s peristaltic pump draws liquid into
the sample cell, takes a reading, returns the liquid
to the sample source or discharges it to a drain,
and then begins the sequence again. This optional
accessory is ideal for situations when small sample
volumes are necessary, or repetitive tests are
required in rapid succession. The Sipper Module
options are easily programmed with the DR/4000
menu. Available with a 1-inch or a 1-cm pathlength
quartz cell.

To measure smaller sample volumes for biological,

pharmaceutical and other research applications, the
MicroSipper Module is ideal. This optional module
is plumbed at the factory with a 1-cm, 50 µL quartz
flow cell. A peristaltic pump delivery system assures
repeatability when you’re working with batch volumes.

12
Flow-Cell Module
This module speeds the measurement of multiple
samples; eliminates errors caused by using different,
unmatched sample cells; and contributes to high
sensitivity and accurate measurement of very low
concentrations. The Flow-Cell Module, designed to
increase precision, also prevents contamination or
dilution between successive samples. Available with
a 1-inch or 1-cm pathlength quartz cell.

COD/Test ‘N Tube™/UniCell™
Carousel Adapter
This five-position, 16-mm sample cell adapter is designed
to provide a way to group, record and quickly perform
several sample measurements at a time. Configured to
accept vials containing chemical oxygen demand,
Test ‘N Tube™ (chlorine, total nitrogen, nitrate, nitrite,
ammonia, phosphorus), and UniCell™ (ammonium, nitrate,
total nitrogen, phosphate, and sulfate) premeasured
reagents, this carousel speeds testing with minimal human
intervention. Depending on the setup menu selected by
the analyst, the DR/4000 automatically rotates the sample
cells and reads the concentrations in a preprogrammed
sequence. An alert sounds when the readings are complete.

Temperature Control Module

This optional module is ideal for samples that need to be
kept at constant temperatures or for kinetic or biological
research. It uses a 1-cm quartz cell and controls the
sample temperature to within ±0.5ºC from 15 to 50ºC.

Technical Support
Whether you need advice about ordering reagents,
detecting interferences or entering your own calibrations,
technical support staff are as close as your telephone. For
intensive training on the DR/4000 and other Hach systems,
contact the dealer or distributor serving your area.

Extended Warranty
and Certification
In addition to the one-year
warranty offered on the DR/4000,
Hach also offers an extended
warranty agreement, which covers
parts and service for your instrument
for an additional one to three
years. Performance verification
services are also available to ensure
that your instrument meets factory
specifications.

13
DR /4000 UV - VIS
Spectrophotometer

Specifications:
Wavelength range:
DR/4000 U: 190 to 1100 nm
DR/4000 V: 320 to 1100 nm
Wavelength accuracy: ±1 nm
Monochromator Design: Seya-Namioka split-beam
monochromator
Wavelength calibration: Internal, automatic
at turn-on with visual
feedback
Wavelength repeatability: ±0.1 nm
Wavelength resolution: 0.1 nm
Grating: 1200 lines/mm;
8 nm/mm dispersion
Wavelength slew rate: 15,000 nm/minute
Spectral bandwidth: 4 nm nominal,
5 nm maximum,
Source lamp: Deuterium (DR/4000 U
only); Gas-filled tungsten
(DR/4000 U and DR/4000 V)
Source lamp change: Automatic changeover
Display: Backlit, graphic, liquid
crystal display with
adjustable viewing angle,
contrast control
Photometric range: -3.0 to 3.0 ABS
Photometric linearity: ±0.002 ABS
Stray light: >3.3 ABS, <0.05 %T
at 220 nm and 340 nm
Readout modes: Concentration, absorbance,
% transmittance
External outputs: Parallel or RS-232 Serial
Sample compartment: Modular, easily accessible,
interchangeable for diverse
testing requirements
Line power: AC line power selectable for
95 to 240 Vac, 50/60 Hz
Dimensions: 42W x 29D x 16H cm
(16.5 x 11.4 x 6.3 in)
Weight: 9 kg (20 lb.)
Compliance: European CE mark, ETL and
ETLc safety marks

Specifications are subject to change without notice.

14
Catalog Information:
48000-00 DR/4000 U Spectrophotometer
Visible and UV spectrum. Includes 1-inch matched sample cells; 1-cm
matched quartz sample cells; Single Cell Module; AccuVac® and 16-mm
vial adapters; 1-inch and 1-cm cell adapters; Carousel Module; and
carousel inserts (1-inch, four-position and 1-cm, six-position); dust
cover; replacement lamp kit; illustrated manual set; and power cord.
48000-02 DR/4000 U Spectrophotometer (European model)
Includes continental European power cord and fuses.
48100-00 DR/4000 V Spectrophotometer
Visible spectrum only. Includes 1-inch matched sample cells; Single Cell
Module; AccuVac® and 16-mm vial adapters; 1-inch and 1-cm cell
adapters; dust cover, replacement lamp kit; illustrated manual set; and
power cord.
48100-02 DR/4000 V Spectrophotometer (European model)
Includes continental European power cord and fuses.
48090-03 Sipper Module, 1-inch quartz sample cell
48090-06 Sipper Module, 1-cm quartz sample cell
48090-07 Sipper Module, Micro 1-cm pathlength quartz cell
48070-05 Flow-Cell Module, 1-cm quartz cell
48070-04 Flow-Cell Module, 1-inch quartz cell
48070-08 Temperature Control Module
48070-02 Carousel Module Kit
48070-01 Single Cell Module Kit
48190-00 1-inch Square Cell Adapter
48105-00 1-inch Carousel Adapter, four position
48115-00 1-cm Carousel Adapter, six position
48186-00 5-cm Single Cell Adapter
48118-00 10-cm Single Cell Adapter
48588-00 1-cm Microcell Adapter
48584-00 1-cm Square Cell Adapter
48274-00 COD/TNT/UniCell™, 16-mm Carousel Adapter,
five position
48189-00 COD/TNT, 16-mm Round Cell Adapter
48187-00 AccuVac®, 1-inch Single Round Cell Adapter
26126-02 1-inch Glass Sample Cell, matched pair with stoppers
26659-02 1-inch Precision Matched Sample Cells, matched pair
26659-04 1-inch Precision Matched Sample Cells, set of four
26659-08 1-inch Precision Matched Sample Cells, set of eight
24102-12 1-inch Polystyrene Sample Cell with cap, 12/pk
20951-00 1-cm Glass Sample Cells, matched pair, square
26295-00 1-cm Low Volume microcell, plastic, disposable,
100/pk (1.5mL)
48228-00 1-cm Quartz Sample Cell, 1 pair with caps
26244-50 5-cm Quartz Sample Cell with cap
26292-50 5-cm Optical Glass Sample Cell with cap
26244-01 10-cm Quartz Sample Cell with cap
26292-01 10-cm Glass Sample Cell with cap
48129-00 Computer Interface Cable, 6 ft.
49665-00 HachLink™ Software
49503-00 Printer Cable
49544-00 Software Upgrade Package
 An exceptional, complete
laboratory analysis
system that includes a
superior instrument,
prepared reagents, proven
methods, complete
instructions and personal
service for running

DR /4000 procedures for

Water •

UV - VIS Wastewater
Boiler/cooling water
•
•

Spectrophotometer Environmental testing

Water conditioning
•
•
Agriculture •
Foods/beverages •
Chemicals •
Aquaculture •
Pharmaceuticals •
Paint and coatings •
Pulp and paper •
Education •
Research •

The DR/4000 increases

productivity, expands testing
capabilities, and provides
accuracy and convenience
in a total solution backed
In the United States, contact: by the experience and
reputation of Hach.
HACH COMPANY
P.O. Box 389
Loveland, Colorado 80539-0389 For current price information,
U.S.A. technical support and ordering
Telephone: 800-227-4224
Fax: 970-669-2932 assistance, contact the
E-mail: [email protected] Hach office or distributor
http://www.hach.com
serving your area.
All other inquiries, contact: Your distributor is:
HACH COMPANY
P.O. Box 389
Loveland, Colorado 80539-0389
U.S.A.
Telephone: 970-669-3050
Fax: 970-461-3939
E-mail: [email protected]
http://www.hach.com

Lit. No. 5124

H42 Printed in U.S.A.
© Hach Company, 2004. All rights reserved.