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#Botany Lab Manual MDFD 2024 - 25

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0% found this document useful (0 votes)
200 views

#Botany Lab Manual MDFD 2024 - 25

Uploaded by

albinjilmon
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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MITOSIS

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PROPHASE
Identification
The given stage is prophase of mitosis.
Reasons
Initiation of condensation of chromatin material.
 Nuclear membrane and nucleolus starts disappear.
 Condensation of chromatin material results in the formation of compact
mitotic chromosome.
Spindle fibres begins to appear.

Nuclear membrane

Chromatin material

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METAPHASE
Identification
The given stage is metaphase of mitosis.
Reasons
 Chromosomes are arranged at the equator of the cell.

 Spindle fibres attach to kinetochores of chromosomes.

The arms of the chromosomes are directed towards the opposite poles.
 Nuclear membrane and nucleolus are absent.
Polar region

Chromosome

Equator

Spindle fibres

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Identification ANAPHASE
The given stage is anaphase of mitosis.
Reasons

Centromeres split and chromatids separate.


 Chromatids move to opposite poles.
The arms of the chromosomes are directed to the equator of the cell.
 Centromere of each chromosome remains directed towards the pole.

Polar region

Equator

Chromatids
Spindle fibres

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TELOPHASE
Identification
The given stage is telophase of mitosis.
Reasons
Nuclear membrane and nucleolus reappear.
Spindle fibres disappear.
Two daughter nuclei present in the same cell.
 Biginning of cytokinesis.
 Chromosome uncoil into chromatin fibres.

Chromatin material

Nuclear membrane

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PLANT ANATOMY

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DICOT STEM
Ground plan
Identification
Epidermis
The given material is Dicot stem Hypodermis
Identification features of dicot stem Endodermis
 Vascular bundles are conjoint. Bundle cap
 Xylem is endarch Phloem
Hence stem Pith
 Cortex is heterogenous. Cambium
Vascular bundles are limited in number.
Vascular bundles are arranged in broken ring manner. Xylem
Vascular bundles are open with cambium.
 Collenchymatous hypodermis. A portion enlarged Epidermal hair
 Sclerenchymatous bundle cap is present. Cuticle
Hence Dicot. Epidermis
Collenchyma
Chlorenchyma

Parenchyma

Endodermis
Bundle cap

Phloem
Cambium

Metaxylem
Protoxylem

Pith

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MONOCOT STEM
Ground plan
Identification Epidermis
The given material is Monocot stem Hypodermis
Identification features of monocot stem
Vascular bundles are conjoint. Vascular bundles
Xylem is endarch.
Hence stem
Ground tissue
Undifferentiated ground tissue.
Vascular bundles are numerous.
Vascular bundles are scattered.
A portion enlarged
Vascular bundles are closed without cambium.
Cuticle
Sclerenchymatous hypodermis. Epidermis
Sclerenchymatous bundle sheath is present. Sclerenchyma
Protoxylem lacuna is present.
Chlorenchyma
Hence monocot.
Ground tissue

Bundle sheath

Phloem
Metaxylem

Protoxylem
Protoxylem lacuna

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DICOT ROOT
Identification Ground plan
The given material is Dicot root.
Root hair
Identification features of Dicot root
Cortex
Vascular bundles are radially arranged. Endodermis
Homogenous cortex.
Pericycle
 Xylem is exarch. Pith
Hence root. Phloem
Xylem
Xylem and phloem groups are limited in number.
Xylem vessels are polygonal in shape.
A portion enlarged
 Pith is reduced or absent.
Root hair
Hence Dicot
Epidermis

Cortex

Endodermis
Pericycle

Metaxylem
Protoxylem
Pith
Phloem

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MONOCOT ROOT
Identification Ground plan
The given material is monocot root.
Root hair
Identification features of monocot root.
Epidermis
Vascular bundles are radially arranged. Endodermis
Homogenous cortex. Pericycle
Xylem is exarch. Metaxylem
Phloem
Hence root.
Protoxylem
 Xylem and phloem groups are numerous in number.
Xylem vessels are round in shape.
 Pith is large.
A portion enlarged
Hence monocot.
Root hair
Epidermis

Cortex

Endodermis
Pericycle
Phloem

Pith
Metaxylem
Protoxylem

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TAXONOMY

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SOLANACEAE
Floral Characters
 Calyx : 5 Sepals, Gamosepalous, Persistent, Valvate aestivation.

 Corolla : 5 Petals, Gamopetalous, Valvate aestivation.


Stigma
 Androecium : Stamens five, Epipetalous. Style
 Gynoecium : Bicarpellary, Bilocular, Syncarpous, Superior ovary, Anther
Filament
Axile placentation.
Ovary

Single flower L.S of flower

Floral diagram Floral Formula

Prepared by Midhun NK,KKMHSS,Cheekode,Malappuram(dt)

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ANTHER

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C.S. OF ANTHER
Identification
The given material is cross section of young anther.
Epidermis
The outer most layer is epidermis.
Epidermis is followed by endothecium,middle layers and tapetum. Endothecium
Tapetum is nutritive in function.
Connective
 Microspores developed to form pollengrains.

Sporogenous-
tissue
Tapetum

Middle layers

Enlarged view of one microsporangium

Epidermis

Endothecium

Middle layers

Microspore-
mother cells
Tapetum

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ECOLOGICAL INTERACTIONS

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MUTUALISM-LICHEN
Identification
The ecological interaction is mutualism in lichen.
Description
Lichens are symbiotic association between algae and fungi.
The algal component is known as phycobiont and fungal component
as mycobiont.
 Algae prepare food for fungi and fungi provide shelter and absorb Host
mineral nutrients and water for its partner.
 Both organisms get benefit (++ interaction).

Thallus

Apothecium

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Identification COMMENSALISM - VANDA
The ecological interaction is commensalism in Vanda.
Description
Vanda is an epiphyte which grows on other plants only for shelter.
 In this interaction vanda is benefitted and the host is neither harmed
nor benefitted.(+ 0 interaction).
 Specialised roots are present in this plant and are called velamen roots.
Velamen roots helps to absorb water and nutrients from the atmosphere.
 It does not take nutrients and water from the host.

Vanda

Clinging roots

Host

Velamen root

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PARASITISM - LORANTHUS
Identification
The ecological interaction is parasitism in Loranthus.
Description
 Parasitism is the interaction between two organisms in which one gets
benefit and the other is harmed (+ -).
 Loranthus is a partial stem parasite.

 In this relation parasite is benefitted and the host is harmed.


Water and minerals are absorbed from the host plant by using specialised -
roots called haustoria.

Loranthus

Host
Haustorium

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Identification
PARASITISM - CUSCUTA
The ecological interaction is parasitism in cuscuta.
Description
Parasitism is the interaction between two organisms in which one gets
benefit and the other is harmed(+ -).
 Cuscuta is a total stem parasite with yellow stem and no leaves.
In this relation parasite is benefitted and the host is harmed.
Flower
Nutrients and water are absorbed from the host plant by using
specialised roots called haustoria.
These roots absorb water and menerals from the xylem of host plant.
Haustorium

Cuscuta

Host

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BIOTECHNOLOGY

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Identification BIO REACTOR
The given material is bioreactor.
Description
 Bioreactors are large vessels in which raw materials are biologically
converted in to specific products,enzymes etc.
It is used to obtain foreign gene products in large quantities.
Bioreactor has an agitator system,an oxygen delivery system,a foam
control system and sampling port for periodic removal of cultured
products.
 The most commonly used bioreactors are of stirring type.

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Identification CLONING VECTOR
The given material is cloning vector pBR322.
Description
 Cloning vectors are used to introduce the foreign DNA fragments in to
the host cell.
A cloning vector contains origin of replication,recognition
sequence and selectable markers.

 pBR322 is an artificially reconstructed plasmid vector.

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