#Botany Lab Manual MDFD 2024 - 25
#Botany Lab Manual MDFD 2024 - 25
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PROPHASE
Identification
The given stage is prophase of mitosis.
Reasons
Initiation of condensation of chromatin material.
Nuclear membrane and nucleolus starts disappear.
Condensation of chromatin material results in the formation of compact
mitotic chromosome.
Spindle fibres begins to appear.
Nuclear membrane
Chromatin material
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METAPHASE
Identification
The given stage is metaphase of mitosis.
Reasons
Chromosomes are arranged at the equator of the cell.
The arms of the chromosomes are directed towards the opposite poles.
Nuclear membrane and nucleolus are absent.
Polar region
Chromosome
Equator
Spindle fibres
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Identification ANAPHASE
The given stage is anaphase of mitosis.
Reasons
Polar region
Equator
Chromatids
Spindle fibres
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TELOPHASE
Identification
The given stage is telophase of mitosis.
Reasons
Nuclear membrane and nucleolus reappear.
Spindle fibres disappear.
Two daughter nuclei present in the same cell.
Biginning of cytokinesis.
Chromosome uncoil into chromatin fibres.
Chromatin material
Nuclear membrane
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PLANT ANATOMY
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DICOT STEM
Ground plan
Identification
Epidermis
The given material is Dicot stem Hypodermis
Identification features of dicot stem Endodermis
Vascular bundles are conjoint. Bundle cap
Xylem is endarch Phloem
Hence stem Pith
Cortex is heterogenous. Cambium
Vascular bundles are limited in number.
Vascular bundles are arranged in broken ring manner. Xylem
Vascular bundles are open with cambium.
Collenchymatous hypodermis. A portion enlarged Epidermal hair
Sclerenchymatous bundle cap is present. Cuticle
Hence Dicot. Epidermis
Collenchyma
Chlorenchyma
Parenchyma
Endodermis
Bundle cap
Phloem
Cambium
Metaxylem
Protoxylem
Pith
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MONOCOT STEM
Ground plan
Identification Epidermis
The given material is Monocot stem Hypodermis
Identification features of monocot stem
Vascular bundles are conjoint. Vascular bundles
Xylem is endarch.
Hence stem
Ground tissue
Undifferentiated ground tissue.
Vascular bundles are numerous.
Vascular bundles are scattered.
A portion enlarged
Vascular bundles are closed without cambium.
Cuticle
Sclerenchymatous hypodermis. Epidermis
Sclerenchymatous bundle sheath is present. Sclerenchyma
Protoxylem lacuna is present.
Chlorenchyma
Hence monocot.
Ground tissue
Bundle sheath
Phloem
Metaxylem
Protoxylem
Protoxylem lacuna
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DICOT ROOT
Identification Ground plan
The given material is Dicot root.
Root hair
Identification features of Dicot root
Cortex
Vascular bundles are radially arranged. Endodermis
Homogenous cortex.
Pericycle
Xylem is exarch. Pith
Hence root. Phloem
Xylem
Xylem and phloem groups are limited in number.
Xylem vessels are polygonal in shape.
A portion enlarged
Pith is reduced or absent.
Root hair
Hence Dicot
Epidermis
Cortex
Endodermis
Pericycle
Metaxylem
Protoxylem
Pith
Phloem
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MONOCOT ROOT
Identification Ground plan
The given material is monocot root.
Root hair
Identification features of monocot root.
Epidermis
Vascular bundles are radially arranged. Endodermis
Homogenous cortex. Pericycle
Xylem is exarch. Metaxylem
Phloem
Hence root.
Protoxylem
Xylem and phloem groups are numerous in number.
Xylem vessels are round in shape.
Pith is large.
A portion enlarged
Hence monocot.
Root hair
Epidermis
Cortex
Endodermis
Pericycle
Phloem
Pith
Metaxylem
Protoxylem
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TAXONOMY
53 54
SOLANACEAE
Floral Characters
Calyx : 5 Sepals, Gamosepalous, Persistent, Valvate aestivation.
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ANTHER
57 58
C.S. OF ANTHER
Identification
The given material is cross section of young anther.
Epidermis
The outer most layer is epidermis.
Epidermis is followed by endothecium,middle layers and tapetum. Endothecium
Tapetum is nutritive in function.
Connective
Microspores developed to form pollengrains.
Sporogenous-
tissue
Tapetum
Middle layers
Epidermis
Endothecium
Middle layers
Microspore-
mother cells
Tapetum
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ECOLOGICAL INTERACTIONS
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MUTUALISM-LICHEN
Identification
The ecological interaction is mutualism in lichen.
Description
Lichens are symbiotic association between algae and fungi.
The algal component is known as phycobiont and fungal component
as mycobiont.
Algae prepare food for fungi and fungi provide shelter and absorb Host
mineral nutrients and water for its partner.
Both organisms get benefit (++ interaction).
Thallus
Apothecium
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Identification COMMENSALISM - VANDA
The ecological interaction is commensalism in Vanda.
Description
Vanda is an epiphyte which grows on other plants only for shelter.
In this interaction vanda is benefitted and the host is neither harmed
nor benefitted.(+ 0 interaction).
Specialised roots are present in this plant and are called velamen roots.
Velamen roots helps to absorb water and nutrients from the atmosphere.
It does not take nutrients and water from the host.
Vanda
Clinging roots
Host
Velamen root
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PARASITISM - LORANTHUS
Identification
The ecological interaction is parasitism in Loranthus.
Description
Parasitism is the interaction between two organisms in which one gets
benefit and the other is harmed (+ -).
Loranthus is a partial stem parasite.
Loranthus
Host
Haustorium
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Identification
PARASITISM - CUSCUTA
The ecological interaction is parasitism in cuscuta.
Description
Parasitism is the interaction between two organisms in which one gets
benefit and the other is harmed(+ -).
Cuscuta is a total stem parasite with yellow stem and no leaves.
In this relation parasite is benefitted and the host is harmed.
Flower
Nutrients and water are absorbed from the host plant by using
specialised roots called haustoria.
These roots absorb water and menerals from the xylem of host plant.
Haustorium
Cuscuta
Host
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BIOTECHNOLOGY
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Identification BIO REACTOR
The given material is bioreactor.
Description
Bioreactors are large vessels in which raw materials are biologically
converted in to specific products,enzymes etc.
It is used to obtain foreign gene products in large quantities.
Bioreactor has an agitator system,an oxygen delivery system,a foam
control system and sampling port for periodic removal of cultured
products.
The most commonly used bioreactors are of stirring type.
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Identification CLONING VECTOR
The given material is cloning vector pBR322.
Description
Cloning vectors are used to introduce the foreign DNA fragments in to
the host cell.
A cloning vector contains origin of replication,recognition
sequence and selectable markers.
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