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GPT Alat

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26 views

GPT Alat

Uploaded by

James
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© © All Rights Reserved
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ALAT / GPT

5+1 liquid

Cat.No Package Size REFERENCE VALUES (IFCC / 37°C)


GP460S29 4 x 50 mL 2 x 20 mL Women < 31 U/L
GP4120S60 Men < 41 U/L
4 x 100 mL 1 x 80 mL
GP2150S138 2 x 125 mL 1 x 50 mL
Note: It is recommended for each laboratory to establish and
GP240H129 Hit I 4 x 50 mL 2 x 20 mL maintain its own reference values. The given data are only an
GP480H229 Hit II 4 x 100 mL 4 x 20 mL indication.

METHOD PROCEDURES
Reagents can be used manually (see method below) and
Optimized, UV, Kinetic. IFCC
on most analyzers. Applications are available on request.
PRINCIPLE
Wavelength : 340 nm, Hg 334 nm, Hg 365 nm
Kinetic determination of Alaninaminotransferase
Temperature : 37°C,
(ALAT) based upon IFCC recommendations:
Cuvette : 1 cm light path
Read against air.
L-Alanin+ 2-Oxoglutarat < ALAT > L-Glutamat + Pyruvat
Pyruvat + NADH + H +
< LDH > L-Lactat + NAD+ • Sample Start Procedure / Monoreagent
ALAT = Alaninaminotransferase Working reagent : 1 mL
LDH = Lactate dehydrogenase
Sample : 100 µL

REAGENTS COMPOSITION Mix and after a 1 minute incubation, measure the


R1: TRIS-Buffer (pH 7,5) 100 mmol/l
change of absorbance per minute (∆A/min) during
L-Alanine 500 mmol/l
LDH ( Lactatdehydrogenase) ≥ 1200 U/l 3 minutes.
R2: 2-Oxoglutarate 15 mmol/l
• Start Reagent Procedure/R1-R2
NADH 0,18 mmol/l

R1 : 1 mL
- PRECAUTIONS Sample : 100 µL
- For in vitro diagnostic use only. Mix and wait 1 minute
- The reagents contain < 0,95g/L sodium azide.
Avoid contact with skin and/or mucous R2 : 200 µL
membranes ! Mix and after a 1 minute incubation, measure the
- Use clean or single use glass/plastic material change of absorbance per minute (∆A/min) during
only to avoid contaminations. 3 minutes.

STABILITY OF REAGENTS
When stored at 2 – 8 °C and protected from light, the
CALCULATION FACTORS
reagents are stable until the expiry date printed on
Monoreagent Start reagent
the labels. 340 nm : Activity (U/L) = ∆A/min. x 1 745 2064
334 nm : Activity (U/L) = ∆A/min. x 1 780 2103
PREPARATION OF WORKING REAGENT 365 nm : Activity (U/L) = ∆A/min. x 3 235 3824
- For the “Start Reagent-procedure”
reagents R1 and R2 are ready for use
- For the “Sample Start-procedure” (monoreagent) CALIBRATORS & CONTROLS
- mix R1 + R2 = 5 + 1 For the calibration of automated analyzers Inmesco
Stability : 1 month at 2 – 8 °C Multicalibrator Introcal is recommended, for quality
5 days at 18 – 25 °C control use Inmesco normal and abnormal control,
Intronorm I and Intronorm II.
SAMPLES
Serum free of hemolysis , plasma (EDTA/heparin)

L-S 04/2009 page 1/2


PERFORMANCE DATA (37°C) BIBLIOGRAPHY
- Analytical range 1. Thomas L. Alanine aminotransferase (ALT), Aspartate
The reagent is linear up to ALAT activities aminotransferase (AST). In: Thomas L, editor. Clinical
st
corresponding to a ∆A/min of 0,16 at 340 nm and Laboratory Diagnostics. 1 ed. Frankfurt: TH-Books
334 nm. If ∆A/min exceeds 0.16 at 340 nm, repeat Verlagsgesellschaft; 1998. p. 55-65.
2. Moss DW, Henderson AR. Clinical enzymology. In:
the test using serum diluted 1/10 with sodium
Burtis CA, Ashwood ER, editors. Tietz Textbook of
chloride solution (9 g/L).Multiply result by 10. Clinical Chemistry. 3
rd
ed. Philadelphia: W.B
Saunders Company; 1999. p. 617-721.
- Sensitivity 3. Bergmeyer HU, Horder M, Rej R. International
The sensitivity is equal to 4 U/L Federation of Clinical Chemistry (IFCC) Scientific
Committee, Analytical section: approved
- Precision recommendation (1985) on IFCC methods for the
measurement of catalytic concentration of enzymes.
Within-run reproducibility Part 3. IFCC method for alanine aminotransferase (L-
Within series Mean Standard- CV alanine:2-oxoglutarate aminotransferase, EC 2.6.1.2).
n = 20 Value deviation [%]
[U/L] [U/L]
J Clin Chem Clin Biochem 1986;24:481-95.
4. Lorentz K, Röhle G, Siekmann L. Einführung der
Sample 1 38,4 0,51 1,32
Sample 2 143 1,95 1,36 neuen Standardmethoden 1994 zur Bestimmung der
Sample 3 111 1,27 1,14 katalytischen Enzymkonzentrationen bei 37 °C. DG
Klinische Chemie Mitteilungen 1995;26:190-2.
Between-run reproducibility
Day to day Mean Standard- CV
n = 20 Value deviation [%] SYMBOLS USED
[U/L] [U/L]
Sample 1 36,0 0,78 2,15
Sample 2 146 0,94 0,65
Sample 3 109 1,40 1,28 For in vitro diagnostic medical use

- Correlation
A comparative study has been performed between Batch Code
the Inmesco method and another commercial
reagent on 30 human serum samples. The
parameters of linear regression are as follows: Use by

Correlation coefficient r = 0.999


Linear regression: y = 1.00x + 0.0 U/L Temperature limitation

INTERFERENCES
-Ascorbic Acid: No interference until 30 mg/L

-Triglycerides: No interference until 2000 mg/dL

-Bilirubin: no interference up to 40 mg / dL

L-S 04/2009 page 2/2

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