CytoSinctTM

Cell Isolation and Activation Solution

Highly Efficient
Nano-sized GMP Reagent
Clinical Grade Tubing Set
Automatic Instrument
Efficient and gentle cell isolation is essential for researching specific cell populations, and for developing lifesaving

cell and gene therapies products. Purity, recovery and viability are among the most important characteristics of

cells after isolation.

GenScript has renowned expertise in developing magnetic beads products for bio separation of various types of

biomolecules. CytoSinctTM magnetic beads are powered by immunomagnetic cell separation technology to isolate

desired cell populations, by combining the specificity and selectivity of antibody based purification with gentleness

of nanoparticles.

CytoSinct™ manual cell isolation platform is composed of antigen-specific paramagnetic nanobeads, column and

magnetic separator. CytoSinctTM beads are nanometer-sized, coated with biodegradable matrix, nontoxic, easy to

use, and enable highly efficient cell isolation. CytoSinctTM columns amplify the magnetic field and enable efficient

separation with minimum labeling. The separated cells are compatible with most downstream applications including

cell culture, activation, expansion, flow cytometry analysis, and translational research.

Armed with the flexibility and specificity of CytoSinct™ cell isolation solution, you can use variety of starting

materials including PBMC or Leukoapheresis, for the enrichment and depletion of your desired cell type. Every time

using CytoSinct™ isolation products, you will obtain the isolated sample with high purity and recovery.

Stably conjugated
antibody

Biosafe matrix

Magnetic Core

100 nm
CytoSinct™ Nanobeads

Figure 1 : Schematic diagram showing the structure and size Figure 2 : CytoSinct™ cell isolation platform includes cell isolation
of CytoSinctTM Nanobeads. nanobeads, columns and magnetic separator.

Learn more at https://www.genscript.com/cell-separation.html

For more information contact [email protected]
 Product Highlights

Ultra High High Nano- Bio- Bio- Cost

Purity Recovery Viability sized degradable compatible Efficient

Product Advantages
Advantages GenScript Vendor A Vendor B Vendor C
Cell isolation performance (purity& recovery rate) N/A
Cell activation (cell expansion rate/TCM+TSCM amount)
Clinical use
No Bead-removal Step
Safety /
Cost Efficiency
Customized beads service

Workflow
Genscript’s CytoSinctTM Nanobeads purify cells using column based cell isolation.

N S N S N S

1
Incubate the starting
2
Wash and resuspend
3
Assemble column onto
4
Add Isolation Buffer
5
Remove magnet from
material with CytoSinctTM the cells in the magnet. onto column to wash column. Add Isolation
Nanobeads. Isolation Buffer. Load cell suspension out negative cells. Buffer and harvest
containing both positive cells.
Only the cells expressing the positive and negative
targeted surface marker will
population onto the
bind with the CytoSinctTM
Nanobeads. column.

Only the cells bound to the

CytoSinctTM Nanobeads will be
captured by the magnet.

Learn more at https://www.genscript.com/cell-separation.html

For more information contact [email protected]
 Highly Efficient CD4+CD8+T Cell Isolation from PBMCs

Purity, recovery and viability are among the most important characters of cells post-isolation. CytoSinctTM Nanobeads
are nanometer-sized and can isolate your target cells gently and efficiently, which makes your postisolation cells
suitable for variety of down-stream applications.

A B C D

Figure 3. PBMCs were isolated using CytoSinct™ CD4 Nanobeads, human (RUO) (Cat# L00863) and CytoSinct™ CD8 Nanobeads, human (RUO) (Cat# L00864), the
results shows high purity(A,B), high recovery(C) and high viability(D).

Comparing to the similar products from the competitor, GenScript CytoSinct™ Nanobeads can provide the target
cells with high purity and recovery rate.

A B

C D

Figure 4. Subpopulation phoenotype of post-isolation cells is important for functional and phenotypic studies. Non-T subpopulations like monocyte and NK cells could
impact the efficacy of T-cells or therapeutic CAR-T and TCR-T generated from the enrichment. GenScript CytoSinctTM NanoBeads for CD4 and CD8 T-cell isolation
efficiently minimize non-T composition in the enrichment population.
 Highly Efficient T Cell Isolation from Apheresis

CD4+&CD8+ or CD3+ T cell isolation are both commonly used for the relevant T cell studies. GenScript can provide
high quality of both RUO and GMP grade CytoSinct™ Nanobeads, which can support your studies from bench-side
to bed-side.

A CytoSinctTM CD4&CD8 Nanobeads

CytoSinctTM CD4&CD8 Nanobeads
Competitor-CD4&CD8 B CytoSinctTM CD3 Nanobeads
Competitor-CD4&CD8 Competitor CD3 CytoSinctTM CD3 Nanobeads
Apheresis Start Competitor CD3

Figure 5. Representative data comparing T cells isolation from leukopheresis samples using GenScript CytoSinctTM Nanobeads and competitor products.
A. Shows comparison of pre and post isolation data, purity, recovery, and viability for co-isolation of CD4 and CD8 T cell from apheresis using CytoSinctTM CD4 +
CytoSinctTM CD8 and competitor reagent.
B. Shows comparison of pre and post isolation data, purity, recovery, and viability. CD3 T cell from apheresis using CytoSinctTM CD3 and competitor reagent.

Isolated T cells using CytoSinct™ Nanobeads has a high purity and recovery rate.

Enceed™ — World Leading T Cell Activation Reagent

GenScript Enceed™ T cell activation uses CD3 and CD28 as the activation marker for in vitro activation and
expansion of human T cells. Comparing to the similar products from the competitor, activated T cells using Enceed™
can obtain higher expansion folds and larger total amount of TSCM+TCM, which is greatly beneficial for the
downstream application.

A cC

B D

Figure 6. (A) Isolated CD4+CD8+T cells were activated using the Enceed™ T cell activation reagent from GenScript, comparing with the similar activation product from
the competitor. After the activation, the cell samples were cultured and expanded for 48 hours, and the CD25+ & CD69+ expression were detected by flowcytometry and
shown. (B) To show the expansion status after the activation, isolated T cells were stained with CFSE before and 7 days after the activation. (C) The expansion folds of
the activated T cells were shown along the course of 17 days with the comparison of the activated T cells using competitor's product. (D) The memorial phenotypes of
the activated T cells were monitored and shown on Day 12 and Day 17 after the activation.
 CytoSinct™ column and magnetic separator

CytoSinct™
gL columns

Advantages
· Anti-clogging property: Innovative structure
· Compatibility: compatible with beads from other vendors
· High performance: High purity, recovery and viability cells
CytoSinct™
after separation
gM columns

CytoSinct™ M1 magnet CytoSinct™ L1 magnet

Features
· High Recovery & Fast Isolation
· Flexible Throughput
(single/multi-channel)
CytoSinct™ gStand CytoSinct™ M8 magnet CytoSinct™ L4 magnet
· Easy to operate

Products List of CytoSinct™ column and magnetic separator

CAT.NO. Name of products Standard Column load capacity

D00007 CytoSinct™ gM columns 25 pc Up to 107 magnetically labeled cells from up to 2×108 total cells (each column)

D00008 CytoSinct™ gL columns 25 pc Up to 108 magnetically labeled cells from up to 2×109 total cells (each column)

D00006 CytoSinct™ gStand 1 pc Compatible with all CytoSinct™ Magnets

D00009 CytoSinct™ M1 magnet 1 pc Holds 1 CytoSinct™ gM column

D00010 CytoSinct™ M8 magnet 1 pc Holds 8 CytoSinct™ gM columns

D00011 CytoSinct™ L1 magnet 1 pc Holds 1 CytoSinct™ gL column

D00012 CytoSinct™ L4 magnet 1 pc Holds 4 CytoSinct™ gL columns

 CytoSinctTM 1000 Automated Cell Isolation Instrument

Representative data of Cell Isolation with CytoSinct™ 1000 system

Group CytoSinct™ Nanobeads Positive cell% Instrument Tubing Set type

Competitor CytoSinct™ CD4&CD8 64.40% Competitor Instrument Competitor Tubing Set

GenScript CytoSinct™ CD4&CD8 64.40% GenScript CytoSinct™ 1000 GenScript CytoSinct™ 1000 Tubing Set

Competitor Competitor Competitor

GenScript GenScript GenScript
purity of CD45 + T cells(%)

apheresis apheresis

viability(%)

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GenScript CytoSinct™ 1000 system can achieve a high purity, recovery rate and viability of the target cells.
Higher cell recovery rate is observed, since CytoSinct™ 1000 instrument has a relevant higher elution efficiency.
The closed and sterilized CytoSinct™ 1000 Tubing Set Products are optimized for different applications.

Top Performance Wireless Transmission Data Integrity Customized Service

(21 CFR Part 11)

Sample bag

Pinch valve
Touch screen

Code scanning gun

Magnetic field

Peristaltic pump

Sample storage desk

 Cell Therapy Product List

CytoSinct™ Isolation & Enceed™Activation Nanobeads

Field Cat.No Name Size Capability

L00863 CytoSinct™ CD4 Nanobeads, human 1 mL/0.5 mL Up to 1×109 /5×108 total MNCs

L00864 CytoSinct™ CD8 Nanobeads, human 1 mL/0.5 mL Up to 1×109 /5×108 total MNCs

T Cell L00896 CytoSinct™ CD3 Nanobeads, human 1 mL/0.5 mL Up to 1×109 /5×108 total MNCs

L00897 CytoSinct™ TCR αβ Nanobeads, human 1 mL/7.5 mL Up to 1×109 /2×1010 total MNCs

L00899 Enceed™ T cell Activation, human 1 mL/0.5 mL Up to 2×108 /1×108 T cells

NK Cell L00903 CytoSinct™ CD56 Nanobeads, human 1 mL/0.5 mL Up to 1×109 /5×108 total MNCs

Stem Cell L00967 CytoSinct™ CD34 Nanobeads, human 1 mL Up to 1×109 total MNCs

Universal L00898 CytoSinct™ Streptavidin Nanobeads 1 mL/0.5 ml Up to 1×109 /5×108 total MNCs

Monocytes L00956 CytoSinct™ CD14 Nanobeads, human 1 mL/0.5 ml Up to 1×109 /5×108 total MNCs

B Cell L00957 CytoSinct™ CD19 Nanobeads, human 1 mL/0.5 ml Up to 1×109 /5×108 total MNCs

GMP Grade Nanobeads

Cat.No Name Size Capability

L00932 CytoSinct™ CD4 Nanobeads, human (GMP) 7.5 mL Up to 4×1010 total MNCs

L00933 CytoSinct™ CD8 Nanobeads, human (GMP) 7.5 mL Up to 4×1010 total MNCs

L00934 CytoSinct™ CD3 Nanobeads, human (GMP) 7.5 mL Up to 4×1010 total MNCs

L00935 Enceed™ T cell Activation, human (GMP) 4 mL Up to 1×108 T cells

Consumables and Instruments

Cat.No Name Size Capability

D00006 CytoSinct™ gStand 1 pc Compatible with all CytoSinct™ Magnets

D00007 CytoSinct™ gM columns 25 pc Up to 10 magnetically labeled cells from up to 2×108 total cells (each column)
7

D00008 CytoSinct™ gL columns 25 pc Up to 108 magnetically labeled cells from up to 2×109 total cells (each column)

D00009 CytoSinct™ M1 magnet 1 pc Holds 1 CytoSinct™ gM column

D00010 CytoSinct™ M8 magnet 1 pc Holds 8 CytoSinct™ gM columns

D00011 CytoSinct™ L1 magnet 1 pc Holds 1 CytoSinct™ gL column

D00012 CytoSinct™ L4 magnet 1 pc Holds 4 CytoSinct™ gL columns

D00023 CytoSinct™1000 1U

D00029 CytoSinct™ 1000 Tubing Set 1 pc Up to 60×109 total cells

D00030 CytoSinct™ 1000 LS Tubing Set 1 pc Up to 120×109 total cells

D00031 CytoSinct™ 1000 Depletion Tubing Set 1 pc Up to 120×109 total cells

 Cell Therapy Product List

Cas Nuclease Recommendation

Applications Recommendations Cat. No. Product Names

Z03621 GenCRISPR™ Ultra NLS-Cas9-Research

Gene KO/KI with high editing
Ultra SpCas9 (wt) Z03623 GenCRISPR™ Ultra NLS-Cas9- basic GMP
efficiency
Z03623-GMP GenCRISPR™ Ultra NLS-Cas9-GMP

Z03622 GenCRISPR™ Ultra eSpCas9-2NLS-Research

Gene KO/KI with low off-target Ultra eSpCas9 (mt) Z03624 GenCRISPR™ Ultra eSpCas9-2NLS-basic GMP
effects
Z03624-GMP GenCRISPR™ Ultra eSpCas9-2NLS-GMP

Z03701 GenCRISPR™ Cas9 v1.1

Gene KI with high KI efficiency SpCas9 with optimized NLS
Z03702 GenCRISPR™ Cas9 v1.2

Gene KO/KI following with flow Z03393

SpCas9-eGFP fusion protein GenCrispr NLS-Cas9-EGFP Nuclease
cytometry or other fluorescent assays
Z03467

AsCas12a Z03502 GenCRISPR™ Cas12a (Cpf1) Nuclease

Gene KO/KI with lower MW Cas
LaCas12a Z03753 GenCRISPR™ LbCas12a Nuclease
nucleases
SaCas9 Z03699 GenCRISPR™ SaCas9 2NLS Nuclease

LwaCas13a Z03486 GenCRISPR™ Cas13a (C2c2) Nuclease

LbuCas13a Z03742 GenCRISPR™ LbuCas13a Nuclease

in vitro Diagnostics
AsCas12a Z03502 GenCRISPR™ Cas12a (Cpf1) Nuclease

LaCas12a Z03753 GenCRISPR™ LbCas12a Nuclease

 www.GenScript.com
GenScript USA Inc.
860 Centennial Ave.
Piscataway, NJ 08854 USA

Toll-Free: 1-877-436-7274
Tel: 1-732-885-9188
Fax: 1-732-210-0262
Email: [email protected]

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specifically identified otherwise. This includes all imagery, text and programmatic computer code.
ENCS20231026