COURSE MODULE

Program Title B. Pharmacy

Department Pharmaceutics

Course Title Microbiology

1. NAME OF INSTITUTION : Y. B. CHAVAN COLLEGE OF PHARMACY,
AURANGABAD

2. AFFILIATED UNIVERSITY : DR. BABASAHEB AMBEDKAR

MARATHWADA UNIVERSITY, AURANGABAD

3. DEPARTMENT : PHARMACEUTICS
4. PROGRAM TITLE : B. PHARM.
4.1. Program Outcomes (PO):
PO 01:Pharmacy Knowledge: Possess knowledge and comprehension of the core and basic
knowledge associated with the profession of pharmacy, including biomedical sciences;
pharmaceutical sciences; behavioral, social, and administrative pharmacy sciences; and
manufacturing practices.

PO 02:Planning Abilities: Demonstrate effective planning abilities including time

management, resource management, delegation skills and organizational skills. Develop
and implement plans and organize work to meet deadlines.

PO 03:Problem analysis: Utilize the principles of scientific enquiry, thinking analytically,

clearly and critically, while solving problems and making decisions during daily practice.
Find, analyze, evaluate and apply information systematically and shall make defensible
decisions.

PO 04:Modern tool usage: Learn, select, and apply appropriate methods and procedures,
resources, and modern pharmacy-related computing tools with an understanding of the
limitations.

PO 05:Leadership skills: Understand and consider the human reaction to change, motivation
issues, leadership and team-building when planning changes required for fulfillment of
practice, professional and societal responsibilities. Assume participatory roles as responsible
 citizens or leadership roles when appropriate to facilitate improvement in health and
wellbeing.

PO 06: Professional Identity: Understand, analyze and communicate the value of their
professional roles in society (e.g. health care professionals, promoters of health, educators,
managers, employers, employees).

PO 07: Pharmaceutical Ethics: Honour personal values and apply ethical principles in
professional and social contexts. Demonstrate behavior that recognizes cultural and personal
variability in values, communication and lifestyles. Use ethical frameworks; apply ethical
principles while making decisions and take responsibility for the outcomes associated with
the decisions.

PO 08:Communication: Communicate effectively with the pharmacy community and with

society at large, such as, being able to comprehend and write effective reports, make
effective presentations and documentation, and give and receive clear instructions.

PO 09:The Pharmacist and society: Apply reasoning informed by the contextual knowledge
to assess societal, health, safety and legal issues and the consequent responsibilities relevant to
the professional pharmacy practice.

PO 10:Environment and sustainability: Understand the impact of the professional pharmacy

solutions in societal and environmental contexts, and demonstrate the knowledge of, and
need for sustainable development.

PO 11:Life-long learning: Recognize the need for, and have the preparation and ability to
engage in independent and life-long learning in the broadest context of technological
change. Self-assess and use feedback effectively from others to identify learning needs and to
satisfy these needs on an ongoing basis.
5. COURSE SPECIFICATION :
5.1.Course Identification and General Information

a. Course Title: Microbiology

b. Course Number/Code BP 303 T

c. Credit Hours Theory Practical

45(3 Hrs/Week) 60 (4Hrs. / Week)

d. Study level/semester at which this Third Semester B. Pharm.

course is offered
e. Pre-requisite Basics of Biochemistry, Formulations

f. Co-requisite Pharmaceutics

g. Program in which the course is offered B Pharm

h. Language of teaching the course English

i. Prepared by
Mr. Mohammed Imran Anees
j. Approved by HOD Dr. S.R.Lahoti

5.2.Course Description: Microbiology is the study of all organisms that are invisible to the naked eye-
that is the study of microorganisms. Microorganisms are necessary for the production of bread, cheese,
beer, antibiotics, vaccines, vitamins, enzymes etc. Microbiology has an impact on medicine, agriculture,
food science, ecology, genetics, biochemistry, immunology etc.

5.3.Course Objectives:

• Understand methods of identification, cultivation and preservation of various microorganisms

• Importance of sterilization in microbiology and pharmaceutical industry
• Learn sterility testing of pharmaceutical products.
• Microbiological standardization of Pharmaceuticals.
• Understand the cell culture technology and its applications in pharmaceutical industries.
6.0. Course Outcomes (COs) : (Min. 4 and Max. 6)

(Use Bloom’s Taxonomy words)

CO Code Course outcome
Identify the key growth parameters required by micro-organisms and how to
CO 303.01 identify bacteria and able to cultivation and preservation of various
microorganisms.
Describe the principles and commonly used methods of sterilization and
CO 302.02 disinfection used in the pharmaceutical industry and to plan out the protocols
for sterility checkups in labs and in industries
Describe laminar air flow technology used to prevent contamination of
CO 303.02 pharmaceutical parental and ophthalmic products and to focus on health care of
common individual.
CO 303.04 Describes and explains Microbiological standardization of Pharmaceuticals and
Environmental safety.
Describe the causes and control of microbial spoilage of pharmaceutical
CO 303.05 products and understands and highlights cell culture technology and
applications in pharmaceutical industries
To study the importance of how to maintain environment safety by sterility
CO 303.06 checkup of Labs and Household process and lifelong learning process in day-
to-day life while water and food analysis process.

6.1. Knowledge and Understanding

(Alignment of POs to COs)

CO Code Program Outcome (PO)

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO 303.01 1 1 2 1 -- 1 1 -- 1 1 1
CO 303.02 2 1 2 1 1 2 1 1 2 2 2
CO 303.03 1 1 2 1 --- 2 2 1 2 2 2
CO 303.04 1 1 2 2 -- 3 2 -- 3 2 1
CO 303.05 2 1 1 3 -- 2 2 1 2 2 2

CO 303.06 2 2 2 2 -- 2 2 1 2 2 3

Correlation levels 1, 2 or 3 as defined below:

1: Slight (Low); 2: Moderate (Medium); 3: Substantial (High); If there is no correlation, put ‘-‘
6.2.Teaching and Assessment Methods for achieving learning outcome:

Teaching Strategies(methods)/Tools used Methods of Assessment

Lectures (Constructivist learning) Formative Assessment
Collaborative learning (Discussion) Case study
Project based Learning Class test
Blended learning Multiple choice questions
Inquiry based learning Assignments
Flash cards Seminar
Video Viva Voce
Equipment models Synopsis
Tutorials
Summative Assessment

6.3.Tools for the Teaching and learning

Theory subjects Practical Subjects
 PowerPoints presentation  White boards
 Videos  Glassware
 Flash Card  Chemicals
 Models  Instruments
 Software  Equipment
 Charts  Software
 Smart Boards  Models
 White boards  Plants/Crude Drugs
 Online Platform  Animal
6.4. COURSE CONTENT
Theoretical Aspect:

Order Topic Subtopics list Number Contact

list/units of Hours
Weeks
1 Unit I a) Introduction to Prokaryotes and Eukaryotes 3 and 10
b) Study of ultra-structure and morphological
Half
classification of bacteria, nutritional requirements,
raw materials used for culture media and physical week
parameters for growth, growth curve, isolation and
preservation methods for pure cultures, cultivation
of anaerobes, quantitative measurement of bacterial
growth (total & viable count).
c) Study of different types of phase constrast
microscopy, dark field microscopy and electron
microscopy.

2 Unit II a) Identification of bacteria using staining 3 and 10

techniques (simple, Gram’s &Acid-fast staining) and
Half
biochemical tests (IMViC).
b) Study of principle, procedure, merits, demerits week
and applications of Physical, chemical and
mechanical method of sterilization.
c) Evaluation of the efficiency of sterilization
methods
Equipment’s employed in large scale sterilization.
Sterility indicators.

3 Unit III a) Study of morphology, classification, 3 and 10

reproduction/replication and cultivation of Fungi and
Half
Virus.
b) Classification and mode of action of disinfectants week
c) Factors influencing disinfection, antiseptics and
their evaluation. For bacteriostatic and bactericidal
actions
d) Evaluation of bactericidal & Bacteriostatic.
e) Sterility testing of products (solids, liquids,
ophthalmic and other sterile products) according to
IP, BP and USP.

4 Unit IV Designing of aseptic area, laminar flow equipment’s; 2 and 8

study of different sources of contamination in an
half
aseptic area and methods of prevention, clean area
classification. week
a) Principles and methods of different
microbiological assay. Methods for standardization
of antibiotics, vitamins and amino acids.
b) Assessment of a new antibiotic and testing of
antimicrobial activity of a new substance.
 c) General aspects-environmental cleanliness.

5 Unit V a) Types of spoilage, factors affecting the microbial 2 and 7

spoilage of pharmaceutical products, sources and
half
types of microbial contaminants, assessment of
microbial contamination and spoilage. week
b) Preservation of pharmaceutical products using
antimicrobial agents, evaluation of microbial
stability of formulations.
c) Growth of animal cells in culture, general
procedure for cell culture, Primary, established and
transformed cell cultures.
d) Application of cell cultures in pharmaceutical
industry and research.

TOTAL 45

6.1.Practical Aspects
Order Name of Experiment Number of
Weeks
1 Introduction and study of different equipment’s and processing, e.g., One week
B.O.D. incubator, laminar flow, aseptic hood, autoclave, hot air
sterilizer, deep freezer, refrigerator, microscopes used in
experimental microbiology.
2 Sterilization of glassware, preparation and sterilization of media. One week
3 Sub culturing of bacteria and fungus. Nutrient stabs and slants One week
preparations.
4 Staining methods- Simple, Gram’s staining and acid-fast staining, Five weeks
Negative staining, Monochrome staining (Demonstration with
practical).
5 Isolation of pure culture of micro-organisms by multiple streak Three weeks
plate technique and other techniques.
6 Microbiological assay of antibiotics by cup plate method and other Two weeks
methods
7 Motility determination by Hanging drop method. One week
8 Sterility testing of pharmaceuticals. Three weeks
9 Bacteriological analysis of water Two weeks
10 Biochemical test (IMViC reactions) One week

ASSESSMENT MECHANISM:
 Sr. Assessment Mechanism Week due Marks Proportion of Final
No. Assessment
1 Assignments, Exercises & Home 2nd week of 10 6%
works every month
2 Sessional (Internal Theory exam) As per 15 10%
scheduled
examination
3 Continuous Practical Assessment Weekly during 15 10%
(Sessional Practical exam) practical’s
4 Final exam (theory) As per 75 50%

5 Final exam(practical) University at 35 24%

end of course
Total 150 100%

7.0.STUDENT SUPPORT:

Office hours/week Other procedures

Two hours minimum Phone call, Email and WhatsApp msg

8.0.TEACHER’S AVAILABILITY FOR STUDENT SUPPORT:

Days Monday Tuesday Wednesday Thursday Friday Saturday

Time 4.00-5.00 4.00-5.00 4.00-5.00 4.00-5.00 4.00-5.00 4.00-5.00
pm pm pm pm pm pm
9.0.LEARNING RESOURCES:
Sr. No. Title of Learning Material Details
1 Text books
1) Textbook of Pharmaceutical
Microbiology by chanderkant kokare.

2) W.B. Hugo and A.D. Russel:

Pharmaceutical Microbiology,
Blackwell Scientific publications,
Oxford London.

3) Pelczar, Chan Kreig, Microbiology,

Tata McGraw Hill edn

4) Ananthnarayan : Text Book of

Microbiology, Orient-Longman,
Chennai

2 Essential references (as per syllabus)

1) Edward: Fundamentals of Microbiology
2) W.B.Hugo and A.D. Russel:
Pharmaceutical Microbiology, Blackwell
Scientific publications, Oxford London.

3) Pelczar, Chan Kreig, Microbiology, Tata

McGraw Hill edn

4) Ananthnarayan : Text Book of

Microbiology, Orient-Longman, Chennai

5) N.K.Jain: Pharmaceutical Microbiology,

Vallabh Prakashan, Delhi

6) Bergeys manual of systematic

bacteriology, Williams and Wilkins- A
Waverly company

3 Reference material Hand Written Notes.

4 E-materials and websites Soft copies (word/ Pdf files), PPT’s.

5 Other learning material Handouts

10.0. FACILITIES REQUIRED:
Sr. No. Particular of Facility Required
1 Lecture Rooms (capacity for 60 students)
2 Laboratory (capacity for 20 students)
3 Computing resources: PC with latest version and hardware/software and utilization of
open source and licensed application software
4 Other resources: Appropriate laboratory tools, Chemicals, Glass ware, Apparatus,
Instrumentation

11.0. COURSE IMPROVEMENT PROCESSES:

11.1. Strategies for obtaining student feedback on effectiveness of teaching:
Course delivery evaluation by students using: Questionnaire forms and online
questionnaires

11.2. Other strategies for evaluation of teaching by the instructor or by the

department:
Periodic review by Academic Planning & Monitoring Committee and departmental review
committee, Observations and assistance of colleagues, External assessments by advisors/
examiners and auditors.

11.3. Process for improvement of teaching:

Use of ICT tools, teaching aids, Simultaneous practical orientation and theory classes
(SPOT), Adoption of reflective teaching.

11.4. Describe the planning procedures for periodically reviewing of course effectiveness
and planning for improvement:
Periodic review by departmental meeting, Review of course delivery and outcome through
assessment and feedback from all stake holders.
11.5. Course development plans:
Provide inputs for course improvement and update to University Course development
Committees (Board of Studies)
12.0. INFORMATION ABOUT FACULTY MEMBER RESPONSIBLE FOR THE
COURSE:
Name Dr.Syed Iftequar Ahmed
Location Pharmaceutics Department
Contact Detail (e-mail &cell no.) 9028223979; [email protected]
Office Hours 10:00 AM to 5:00 PM

Name
Mr. Mohammed Imran Anees
(B. Pharm IInd SEM A & B Div)
Location Pharmaceutical-Chemistry Dept.
Contact Detail (e-mail &cell no.) [email protected]
8087611801
Office Hours 10:00 AM to 5:00 PM