10 500 08159I - en - 2011/10

®
Candida IVD

System for the identification of yeasts

SUMMARY AND EXPLANATION WARNINGS AND PRECAUTIONS

API Candida is a standardized system for the • For in vitro diagnostic use and microbiological
identification in 18-24 hours of yeasts, notably those most control.
frequently encountered in clinical microbiology. The • For professional use only.
species which can be identified by the system are • All specimens, yeast cultures and inoculated products
indicated in the Identification Table at the end of this should be considered infectious and handled
package insert. appropriately. Aseptic technique and usual precautions
for handling yeasts should be observed throughout this
®
PRINCIPLE procedure. Refer to "CLSI M29-A, Protection of
The API Candida strip consists of 10 tubes containing Laboratory Workers from Occupationally Acquired
dehydrated substrates, which enable the performance of Infections; Approved Guideline - Current revision". For
12 identification tests (sugar acidification or enzymatic additional handling precautions, refer to "Biosafety in
reactions). Microbiological and Biomedical Laboratories - CDC/NIH
The reactions produced during incubation are revealed by - Latest edition", or to the regulations currently in use in
spontaneous color changes. each country.
The reactions are read visually according to the Reading • Do not use reagents past the expiry date.
Table and identification is obtained by consulting the list of • Before use, check that the packaging and components
profiles in this package insert or using the identification are intact.
software. • Do not use strips which have been damaged : cupules
deformed, desiccant sachet open, etc.
• Allow media to come to room temperature before use.
CONTENT OF THE KIT (Kit for 10 tests)
• Open ampules carefully as follows :
- 10 API Candida strips - Place the ampule in the ampule protector.
- 10 ampules of API NaCl 0.85 % Medium (2 ml) - Hold the protected ampule in one hand in a
- 10 incubation boxes vertical position (white plastic cap upper-
- 10 result sheets most).
- 1 package insert provided in the kit or downloadable - Press the cap down as far as possible.
from www.biomerieux.com/techlib - Position the thumb tip on the striated part of
the cap and press forward to snap off the top
COMPOSITION of the ampule.
- Take the ampule out of the ampule protector
Strip and put the protector aside for subsequent
The composition of the API Candida strip is given in the use.
Reading Table of this package insert. - Carefully remove the cap.
• The performance data presented were obtained using
Medium the procedure indicated in this package insert. Any
API NaCl 0.85 % Sodium chloride 8.5 g change or modification in the procedure may affect the
Medium Demineralized water 1000 ml results.
2 ml • Interpretation of the test results should be made taking
into consideration the patient history, the source of the
specimen, colonial and microscopic morphology of the
REAGENTS AND MATERIAL REQUIRED BUT NOT strain and, if necessary, the results of any other tests
PROVIDED performed.
Reagents / Instrumentation
- McFarland Standard (ref. 70 900), point 3 on the scale STORAGE CONDITIONS
- Mineral oil (ref. 70 100) Strips
- apiweb TM identification software (Ref. 40 011),
The strips should be stored at 2-8°C until the expiry date
(consult bioMérieux) indicated on the packaging.
Material Media
- Pipettes or PSIpettes The media may be stored at 2-30°C until the expiry date
- Ampule protector indicated on the packaging.
- Ampule rack
- Swabs
- General microbiology laboratory equipment

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api® Candida 08159I - en - 2011/10

SPECIMENS (COLLECTION AND PREPARATION) Inoculation of the strip

API Candida is not for use directly with clinical or other • Distribute the prepared yeast suspension into the tubes
specimens. only, avoiding the formation of bubbles (tilt the
The microorganisms to be identified must first be isolated incubation box slightly forward and position the pipette
on a suitable culture medium according to standard or PSIpette on the edge of the cupule).
microbiological techniques. • Cover the first 5 tests (GLU to RAF) and the last test
(URE) with mineral oil (underlined tests) immediately
INSTRUCTIONS FOR USE after inoculating the strip.
Selection of colonies NOTE : The quality of the filling is very important : tubes
Exam the strain to be studied under a microscope to which are insufficiently or excessively full may cause
check that it is indeed a yeast. false positive or false negative results.
The colonies may be isolated on the following media • Close the incubation box.
before using the API Candida strip : • Incubate for 18-24 hours at 36°C ± 2°C in aerobic
- Sabouraud 2 agar (with or without antibiotic) ; conditions.
- Albicans ID 2 agar ;
- Blood agar ; READING AND INTERPRETATION
- If other media are used to isolate the colonies, perform Reading the strip
a subculture on one of the media mentioned above.
After the 18-24-hours of incubation :
Preparation of the strip • Read the reactions by referring to the reading table in
• Prepare the incubation box (tray and lid) and distribute this package insert and record them as + or – on the
about 5 ml of water [demineralized or distilled water, or result sheet.
any water without additives or chemicals which may Warning : Tubes 8 and 9 are bi-functional and enable
release gases (e.g. Cl2, CO2, etc.)] into the honey- 2 reactions to be performed in the same tube :
combed wells of the tray to create a humid atmosphere. - Tube 8 : ßXYL (test no. 8) / ßNAG (test no. 11).
• Record the strain reference on the elongated flap of the - Tube 9 : ßGUR (test no. 9) / ßGAL (test no. 12).
tray. Interpretation
• Remove the strip from its individual packaging.
• Place the strip in the incubation box. • Code the reactions obtained into a numerical profile :
• Discard the desiccant sachet. on the result sheet, the tests are separated into groups
of three and a number 1, 2 or 4 is assigned to each one.
Preparation of the inoculum By adding together the numbers corresponding to
• Open an ampule of API NaCl 0.85 % Medium (2 ml) as positive reactions within each group, a 4-digit numerical
indicated in the paragraph "Warnings and Precautions". profile is obtained.
• Using a pipette or swab, take one or several well- • Identification :
isolated, identical colonies and prepare a suspension This is performed using the database (V 2.1)
with a turbidity equivalent to 3 McFarland : compare with * with the numerical profile :
a turbidity control or measure using a densitometer. It is - Look up the profile in the list in this package insert.
recommended to use young cultures (18-24 hours old). * with the apiweb TM identification software :
• Homogenize the yeast suspension. This suspension - Enter the 4-digit numerical profile manually via the
must be used immediately after preparation. keyboard.
- If there is low discrimination between several species,
complementary tests are indicated (see tables at the
end of this package insert) to separate them. The
results of these tests are taken from literature (or the
ID 32 C database).

7 510 Candida tropicalis

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api® Candida 08159I - en - 2011/10

QUALITY CONTROL
The strips and media are systematically quality controlled at various stages of their manufacture. For those users who
wish to perform their own quality control tests with the strip, it is preferable to use the strain 1. Candida kefyr
®
ATCC 4135 or else one of the following strains :
2. Trichosporon mucoides ATCC 201382* ATCC : American Type Culture Collection,
3. Candida glabrata ATCC 2001 10801 University Boulevard, Manassas,
VA 20110-2209, USA.

GLU GAL SAC TRE RAF ßMAL αAMY ßXYL ßGUR URE ßNAG ßGAL
1. + + + – + – – + – – – +
2. + + + – V + + – + + + –
3. + – – + – – – – – – – –
Profiles obtained after culture of the strains on Sabouraud agar.
* Trichosporon mucoides identified as Trichosporon spp 1 with API Candida.
It is the responsibility of the user to perform Quality Control in accordance with any local applicable regulations.

LIMITATIONS OF THE METHOD PERFORMANCE

• The API Candida system is designed uniquely for the • 646 collection strains and strains of various origins
identification of the species included in the database belonging to species included in the database were
(see Identification Table at the end of this package tested:
insert) i.e. those belonging to the genera Candida, - 97.99 % of the strains were correctly identified
Cryptococcus, Geotrichum, Saccharomyces and (with or without supplementary tests).
Trichosporon. It cannot be used to identify any other - 0.46 % of the strains were not identified.
microorganisms or to exclude their presence. - 1.55 % of the strains were misidentified.
• Only pure cultures of a single organism should be used.
WASTE DISPOSAL
RANGE OF EXPECTED RESULTS Unused reagents may be considered as non hazardous
Consult the Identification Table at the end of this package waste and disposed of accordingly.
insert for the range of expected results for the various Dispose of all used reagents as well as any other
biochemical reactions. contaminated disposable materials following procedures
for infectious or potentially infectious products.
It is the responsibility of each laboratory to handle waste
and effluents produced according to their type and degree
of hazardousness and to treat and dispose of them (or
have them treated and disposed of) in accordance with
any applicable regulations.

WARRANTY
bioMérieux disclaims all warranties, express or implied,
including any implied warranties of MERCHANTABILITY
AND FITNESS FOR A PARTICULAR USE. bioMérieux
shall not be liable for any incidental or consequential
damages. IN NO EVENT SHALL BIOMERIEUX’S
LIABLITY TO CUSTOMER UNDER ANY CLAIM
EXCEED A REFUND OF THE AMOUNT PAID TO
BIOMERIEUX FOR THE PRODUCT OR SERVICE
WHICH IS THE SUBJECT OF THE CLAIM.

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api® Candida 08159I - en - 2011/10

READING TABLE

ACTIVE QTY RESULTS

TESTS REACTIONS/ENZYMES
INGREDIENTS (mg/cup.) NEGATIVE POSITIVE
1) GLU D-glucose 1.4 Acidification (GLUcose)
2) GAL D-galactose 1.4 Acidification (GALactose)
violet yellow
3) SAC D-saccharose 1.4 Acidification (SACcharose)
grey-violet green / grey
4) TRE D-trehalose 1.4 Acidification (TREhalose)
5) RAF D-raffinose 1.4 Acidification (RAFfinose)
4-nitrophenyl-ßD- pale yellow-
6) βMAL 0.08 ß-MALtosidase colorless bright yellow
maltopyranoside
2-chloro-
pale yellow-
7) αAMY 4-nitrophenyl-αD 0.168 α-AMYlase colorless
bright yellow
maltotrioside
4-nitrophenyl-ßD- colorless-very pale yellow / pale yellow-
8) βXYL 0.095 ß-XYLosidase
xylopyranoside blue / green ** bright yellow
4-nitrophenyl-ßD- pale yellow-
9) βGUR 0.063 ß-GlUcuRonidase colorless / blue / green
glucuronide bright yellow
10) URE urea 1.68 UREase yellow-pale orange red
5-bromo-4-chloro-
11) βNAG
3-indoxyl-N-acetyl- 0.09 N-Acetyl-ß-Glucosaminidase colorless / yellow blue / green **
(in tube no. 8) *
ßD-glucosaminide
5-bromo-4-chloro-
12) βGAL
3-indolyl-ßD- 0.0815 ß-GALactosidase colorless / yellow blue / green
(in tube no. 9) *
galactopyranoside

* Tubes 8 and 9 are bi-functional : tube 8 : βXYL (test no. 8) / βNAG (test no. 11)
tube 9 : βGUR (test no. 9) / βGAL (test no. 12)
** Any trace of green in cupule 8 = βXYL (–) βNAG (+)
• The quantities indicated may be adjusted depending on the titer of the raw materials used.

PROCEDURE p. I
LIST OF NUMERICAL PROFILES p. II
TABLES p. III
IDENTIFICATION TABLE p. V
LITERATURE REFERENCES p. VII
INDEX OF SYMBOLS p. VIII

BIOMERIEUX, the blue logo, API and apiweb are used, pending and/or registered trademarks belonging to bioMérieux SA or one of its subsidiaries.
CLSI is a trademark belonging to Clinical and Laboratory Standards Institute, Inc.
ATCC is a trademark belonging to American Type Culture Collection.
Any other name or trademark is the property of its respective owner.

bioMérieux SA bioMérieux, Inc

RCS LYON 673 620 399 Box 15969,
69280 Marcy-l'Etoile / France Durham, NC 27704-0969 / USA
Tel. 33 (0)4 78 87 20 00 Tel. (1) 919 620 20 00
Fax 33 (0)4 78 87 20 90 Fax (1) 919 620 22 11
www.biomerieux.com
api® Candida 08159I - xl - 2011/10

METHODOLOGIE / PROCEDURE / METHODIK / TECNICA / PROCEDIMENTO /

ΔΙΑΔΙΚΑΣΙΑ / METOD / METODYKA

3 McF

API NaCl 0.85 % Medium

GLU URE

API Candida GLU RAF, URE

18:00 - 24:00 36°C ± 2°C

API Candida

+ - + - + -


bioMérieux SA I
api® Candida 08159I - xl - 2011/10

LISTE DES PROFILS NUMÉRIQUES / LIST OF NUMERICAL PROFILES / LISTE DER NUMERISCHEN PROFILE /
LISTA DE PERFILES NUMÉRICOS / LISTA DEI PROFILI NUMERICI / LISTA DE PERFIS NUMÉRICOS /
ΚΑΤΑΛΟΓΟΣ ΑΡΙΘΜΗΤΙΚΩΝ ΠΡΟΦΙΛ / LISTA ÖVER NUMERISKA PROFILER /
LISTE OVER NUMERISKE PROFILER / LISTA PROFILI NUMERYCZNYCH
0 403 Trichosporon spp 2 7 043 Cryptococcus neoformans 2
0 412 Trichosporon spp 2 7 051 C. neoformans 2 / C. neoformans 1
0 413 Trichosporon spp 2 7 053 C. neoformans 2 / Trichosporon spp 1 /
0 417 Trichosporon spp 2 Cryptococcus neoformans 1 (1)
1 000 Candida krusei * (2) 7 100 Candida famata
1 010 Candida krusei * (2) 7 102 Candida albicans
1 100 Candida glabrata 7 104 Candida famata
1 300 Candida glabrata 7 110 Candida tropicalis / Candida albicans (5)
1 402 Trichosporon spp 2 7 112 Candida albicans
1 403 Trichosporon spp 2 7 120 Candida lusitaniae / Candida famata /
1 407 Trichosporon spp 2 Candida guilliermondii (6)
1 412 Trichosporon spp 2 7 200 Saccharomyces cerevisiae
1 413 Trichosporon spp 2 7 204 Candida kefyr
1 416 Trichosporon spp 2 7 213 Trichosporon spp 1 / C. neoformans 1 (1)
1 417 Trichosporon spp 2 7 220 Candida guilliermondii ** (6)
1 443 Trichosporon spp 2 7 224 Candida kefyr
1 453 Trichosporon spp 2 7 241 Cryptococcus neoformans 1
2 403 Trichosporon spp 2 7 243 C. neoformans 1 / C. neoformans 2 /
2 412 Trichosporon spp 2 Trichosporon spp 1 (1)
2 413 Trichosporon spp 2 7 251 Cryptococcus neoformans 1
2 417 Trichosporon spp 2 7 253 C. neoformans 1 / Trichosporon spp 1 (1)
3 000 Geotrichum spp / Candida parapsilosis / 7 300 Saccharomyces cerevisiae
Candida krusei * (2) 7 310 Saccharomyces cerevisiae
3 001 Cryptococcus neoformans 2 7 312 Candida albicans
3 003 C. neoformans 2 / Trichosporon spp 2 (1) 7 320 Candida guilliermondii ** (6)
3 020 Geotrichum spp (2) 7 324 Candida kefyr
3 041 Cryptococcus neoformans 2 7 341 Cryptococcus neoformans 1
3 043 Cryptococcus neoformans 2 7 351 Cryptococcus neoformans 1
3 051 Cryptococcus neoformans 2 7 413 Trichosporon spp 1 / Trichosporon spp 2
3 053 Cryptococcus neoformans 2 7 417 Trichosporon spp 1 / Trichosporon spp 2
3 100 Candida famata / Candida glabrata / 7 420 Candida lusitaniae / Candida guilliermondii (6)
Geotrichum spp (3) 7 441 C. neoformans 1 / C. neoformans 2
3 241 C. neoformans 1 / C. neoformans 2 7 453 Trichosporon spp 1
3 251 Cryptococcus neoformans 1 7 457 Trichosporon spp 1
3 402 Trichosporon spp 2 7 500 Candida lusitaniae / Candida tropicalis /
3 403 Trichosporon spp 2 Candida famata (6)
3 407 Trichosporon spp 2 7 510 Candida tropicalis
3 412 Trichosporon spp 2 7 512 Candida albicans
3 413 Trichosporon spp 2 7 513 Trichosporon spp 1
3 416 Trichosporon spp 2 7 520 Candida lusitaniae
3 417 Trichosporon spp 2 7 530 Candida tropicalis
3 443 Trichosporon spp 2 / C. neoformans 2 (1) 7 553 Trichosporon spp 1
3 453 Trichosporon spp 2 7 557 Trichosporon spp 1
3 641 Cryptococcus neoformans 1 7 600 Candida guilliermondii ** (6)
3 651 Cryptococcus neoformans 1 7 603 Trichosporon spp 1 / C. neoformans 1 (1)
3 653 Trichosporon spp 1 / C. neoformans 1 / 7 611 Trichosporon spp 1 / C. neoformans 1 (1)
Trichosporon spp 2 (1) 7 613 Trichosporon spp 1
5 000 Candida famata / Candida parapsilosis (3) 7 617 Trichosporon spp 1
5 100 Candida famata 7 620 Candida guilliermondii ** (6)
5 104 Candida famata 7 641 Cryptococcus neoformans 1
5 200 Saccharomyces cerevisiae 7 643 C. neoformans 1 / Trichosporon spp 1 (1)
5 241 Cryptococcus neoformans 1 7 647 Trichosporon spp 1
5 243 Cryptococcus neoformans 1 7 651 C. neoformans 1 / Trichosporon spp 1 (1)
5 251 Cryptococcus neoformans 1 7 652 Trichosporon spp 1
5 253 C. neoformans 1 / Trichosporon spp 1 (1) 7 653 Trichosporon spp 1 / C. neoformans 1 (1)
5 300 Saccharomyces cerevisiae / Candida famata (4) 7 657 Trichosporon spp 1
5 641 Cryptococcus neoformans 1 7 671 C. neoformans 1 / Trichosporon spp 1 (1)
5 651 Cryptococcus neoformans 1 7 700 Candida guilliermondii ** (6)
5 653 Trichosporon spp 1 / C. neoformans 1 (1) 7 713 Trichosporon spp 1
6 653 Trichosporon spp 1 7 717 Trichosporon spp 1
7 000 Candida parapsilosis 7 720 Candida guilliermondii ** (6)
7 001 Cryptococcus neoformans 2 7 741 Cryptococcus neoformans 1
7 002 Candida albicans 7 751 C. neoformans 1 / Trichosporon spp 1 (1)
7 003 Cryptococcus neoformans 2 7 753 Trichosporon spp 1
7 012 Candida albicans 7 757 Trichosporon spp 1
7 041 C. neoformans 2 / C. neoformans 1
* Candida inconspicua / Candida norvegensis possible / möglich / posible / possibile / possível / πιθανόν / möjlig / mulig / możliwość
** Candida famata possible / möglich / posible / possibile / possível / πιθανόν / möjlig / mulig / możliwość
(1) cf Table 1 / siehe Tabelle 1 / cf Tabla 1 / cf Tabella 1 / cfr. Quadro 1 / cf Πίνακας 1 / se Tabell 1 / Se Tabel 1 / patrz Tabela 1
(2) cf Table 2 / siehe Tabelle 2 / cf Tabla 2 / cf Tabella 2 / cfr. Quadro 2 / cf Πίνακας 2 / se Tabell 2 / Se Tabel 2 / patrz Tabela 2
(3) cf Table 3 / siehe Tabelle 3 / cf Tabla 3 / cf Tabella 3 / cfr. Quadro 3 / cf Πίνακας 3 / se Tabell 3 / Se Tabel 3 / patrz Tabela 3
(4) cf Table 4 / siehe Tabelle 4 / cf Tabla 4 / cf Tabella 4 / cfr. Quadro 4 / cf Πίνακας 4 / se Tabell 4 / Se Tabel 4 / patrz Tabela 4
(5) cf Table 5 / siehe Tabelle 5 / cf Tabla 5 / cf Tabella 5 / cfr. Quadro 5 / cf Πίνακας 5 / se Tabell 5 / Se Tabel 5 / patrz Tabela 5
(6) cf Table 6 / siehe Tabelle 6 / cf Tabla 6 / cf Tabella 6 / cfr. Quadro 6 / cf Πίνακας 6 / se Tabell 6 / Se Tabel 6 / patrz Tabela 6
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api® Candida 08159I - xl - 2011/10

TABLES / TABELLEN / TABLAS / TABELLE / TABELAS / ΠΙΝΑΚΕΣ / TABELLER / TABELE

TABLE / TABELLE / TABLA / TABELLA / TABELA / TABLE / TABELLE / TABLA / TABELLA / TABELA /
ΠΙΝΑΚΑΣ / TABELL / TABEL 1 ΠΙΝΑΚΑΣ / TABELL / TABEL 4
HYPH / PH ART NAGas SORBITOLas MLZas SORBOSEas
Trichosporon spp + + Saccharomyces cerevisiae – – – (+) –
Cryptococcus neoformans – – Candida famata + + + + (–)

TABLE / TABELLE / TABLA / TABELLA / TABELA / TABLE / TABELLE / TABLA / TABELLA / TABELA /
ΠΙΝΑΚΑΣ / TABELL / TABEL 2 ΠΙΝΑΚΑΣ / TABELL / TABEL 5
ART NAGas SORBITOLas HYPH / PH 37°C CHL MLZas
Geotrichum candidum + – + + – Candida tropicalis – +
Geotrichum capitatum + – – + + Candida albicans + –
Candida parapsilosis – + + + +
Candida inconspicua – – – – +
TABLE / TABELLE / TABLA / TABELLA / TABELA /
Candida norvegensis – – – + +
ΠΙΝΑΚΑΣ / TABELL / TABEL 6
Candida krusei – + – + +
RAFas HYPH / PH SORBOSEas
Candida lusitaniae – + +
TABLE / TABELLE / TABLA / TABELLA / TABELA / Candida famata + – + (–)
ΠΙΝΑΚΑΣ / TABELL / TABEL 3 Candida guilliermondii + V + (–)
HYPH / PH SORBITOLas ART Candida tropicalis – + –
Candida famata – + –
Candida glabrata – – –
LEGENDE / KEY / LEGENDE / LEYENDA / LEGENDA /
Geotrichum candidum + + +
ΕΠΕΞΗΓΗΣΗ / NYCKEL / FORKLARING
Geotrichum capitatum + – +
Candida parapsilosis + + – % de réactions positives / % of positive reactions /
positive Reaktionen % / % de reacciones positivas /
% di reazioni positive / % de reacções positivas /
% θετικών αντιδράσεων / % av positiva reaktioner /
% af positive reaktioner / % reakcji pozytywnych
+ > 90 %
+ (–) 70 - 90 %
V 31 - 69 %
– (+) 10 - 30 %
– < 10 %

Les résultats de ces tests complémentaires sont issus de la littérature ou de la base de données ID 32 C /
The results of these complementary tests are taken from literature or the ID 32 C database /
Die Ergebnisse dieser Zusatztests sind der Literatur oder der Datenbasis ID 32 C entnommen /
Los resultados de estos tests complementarios están extraídos de la literatura o de la base de datos ID 32 C /
I risultati di questi tests complementari sono ottenuti dalla letteratura o dalla base dei dati ID 32 C /
Os resultados destes testes complementares foram extraídos da literatura ou da base de dados ID 32 C /
Τα αποτελέσματα από αυτές τις συμπληρωματικές εξετάσεις λαμβάνονται από την αρθρογραφία ή τη βάση δεδομένων ID 32 C /
Resultaten av dessa kompletterande tester har hämtats från litteraturen eller från databasen ID 32 C /
Resultaterne af disse supplerende tests er taget fra litteraturen eller ID 32 C databasen /
Wyniki tych testów uzupełniających zostały zaczerpnięte z piśmiennictwa oraz bazy danych dla ID 32 C.

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Production de Pseudohyphae/hyphae sur gélose RAT /

Production of Pseudohyphae/hyphae on RAT medium /
Bildung von Pseudohyphen/Hyphen auf RAT Medium /
Producción de Pseudohifas/hifas sobre medio RAT /
Produzione di Pseudoife/ife su agar RAT / c d
HYPH / PH
Produção de Pseudohifas em meio de RAT /
Παραγωγή Ψευδοϋφών/υφών σε υλικό RAT /
Bildning av pseudohyfer/hyfer på RAT medium /
Produktion af Pseudohyphae/hyphae på RAT-medium /
Wytwarzanie pseudostrzępek / strzępek na podłożu RAT
Production de Chlamydospores sur gélose RAT /
Production of Chlamydospores on RAT medium /
Bildung von Chlamydosporen auf RAT Medium /
Producción de Clamidosporas en medio RAT /
Produzione di Clamidospore su agar RAT / c d
CHL
Produção de Clamidosporos em meio de RAT /
Παραγωγή Χλαμυδοσπορίων σε υλικό RAT /
Bildning av klamydosporer på RAT medium /
Produktion af Chlamydosporer på RAT-medium /
Wytwarzanie chlamydospor na podłożu RAT
Production d'Arthrospores sur gélose RAT /
Production of Arthrospores on RAT medium /
Bildung von Arthrosporen auf RAT Medium /
Producción de Artrosporas en medio RAT /
Produzione di Artrospore su agar RAT / c d
ART
Produção de Artrosporos em meio de RAT /
Παραγωγή Αρθροσπορίων σε υλικό RAT /
Bildning av artrosporer på RAT medium /
Produktion af Arthrosporer på RAT-medium /
Wytwarzanie artrospor na podłożu RAT
Croissance à 37°C sur gélose Sabouraud /
Growth at 37°C on Sabouraud agar /
Wachstum bei 37°C auf Sabouraud-Agar /
Crecimiento a 37°C en agar Sabouraud /
Crescita a 37°C su agar Sabouraud / c
37°C
Crescimento a 37° C em gelose Sabouraud /
Ανάπτυξη στους 37°C σε άγαρ Sabouraud /
Tillväxt vid 37°C på Sabouraudagar /
Dyrkning ved 37°C på Sabouraud-agar /
Wzrost na agarze Sabouraud w 37°C
N-Acétyl-Glucosamine Assimilation / N-Acetyl-Glucosamine Assimilation /
N-Acetyl-Glucosamin Assimilation / N-Acetil-Glucosamina Asimilación /
NAGas N-Acetil-Glucosamina Assimilazione / N-Acetil-Glucosamina Assimilação / e
Αφομοίωση N-Ακετυλο-Γλυκοζαμίνης / N-Acetyl-glukosamin assimilation /
N-Acetyl-Glucosamin-Assimilation / Asymilacja N-acetylo-glukozaminy
Raffinose Assimilation / Rafinosa Asimilación / Raffinosio Assimilazione /
RAFas Rafinose Assimilação / Αφομοίωση Ραφφινόζης / Raffinosassimilation / e
Rafinose-Assimilation / Asymilacja rafinozy
Sorbitol Assimilation / Sorbit Assimilation / Sorbitol Asimilación /
SORBITOLas Sorbitolo Assimilazione / Sorbitol Assimilação / Αφομοίωση Σορβιτόλης / e
Sorbitolassimilation / Sorbitol-Assimilation / Asymilacja sorbitolu
Mélézitose Assimilation / Melezitose Assimilation / Melezitosa Asimilación /
MLZas Melezitosio Assimilazione / Melezitose Assimilação / Αφομοίωση Μελεζιτόζης / e
Melezitosassimilation / Melezitose-Assimilation / Asymilacja melezytozy
Sorbose Assimilation / Sorbosa Asimilación / Sorbosio Assimilazione /
SORBOSEas Sorbose Assimilação / Αφομοίωση Σορβόζης / Sorbosassimilation / e
Sorbose-Assimilation / Asymilacja sorbozy

c KREGER VAN RIJ N.J.W. The Yeasts : A Taxonomic Study. (1984) Elsevier, Amsterdam.
d BARNETT J.A., PAYNE R.W., YARROW D. Yeasts : Characteristics and Identification. (1990) Cambridge University Press, London.
e Base de données ID 32 C / ID 32 C database / Datenbasis ID 32 C / Base de datos ID 32 C / Base dei dati ID 32 C /
Base de dados ID 32 C / Βάση δεδομένων ID 32 C / Datenbasen ID 32 C / Database ID 32 C / Baza danych dla ID 32 C.

bioMérieux SA IV
api® Candida 08159I - xl - 2011/10

TABLEAU D'IDENTIFICATION / IDENTIFICATION TABLE / PROZENTTABELLE /

TABLA DE IDENTIFICACION / TABELLA DI IDENTIFICAZIONE / QUADRO DE IDENTIFICAÇÃO /
ΠΙΝΑΚΑΣ ΤΑΥΤΟΠΟΙΗΣΗΣ / IDENTIFIERINGSTABELL / IDENTIFIKATIONSTABEL /
TABELA IDENTYFIKACYJNA

% de réactions positives après 18-24 h à 36°C ± 2°C / % of positive reactions after 18-24 hrs. at 36°C ± 2°C /
% der positiven Reaktionen nach 18-24 h bei 36°C ± 2°C /
% de las reacciones positivas después de 18-24 H a 36°C ± 2°C / % di reazioni positive dopo 18-24 ore a 36°C ± 2°C /
% de reacções positivas após 18-24 h a 36º C ± 2º C / % θετικών αντιδράσεων
μετά από 18-24 ώρες στους 36°C ± 2°C / % positiva reaktioner efter 18-24 timmar vid 36°C ± 2°C /
% af positive reaktioner efter 18-24 timer ved 36°C ± 2°C / % pozytywnych reakcji po 18-24 godzinach w 36°C ± 2°C

API CANDIDA V2.1 GLU GAL SAC TRE RAF βMAL αAMY βXYL βGUR URE βNAG βGAL
Candida albicans 100 100 100 90 3 3 90 0 0 0 99 0
Candida famata 100 70 96 93 1 1 0 1 0 0 0 5
Candida glabrata 100 1 0 100 1 0 0 0 0 0 0 0
Candida guilliermondii ** 100 100 100 35 99 93 0 99 0 0 0 0
Candida kefyr 100 100 100 3 100 0 0 88 0 0 0 100
Candida krusei * 100 5 0 0 0 0 1 0 0 0 0 0
Candida lusitaniae 100 100 100 98 0 99 0 99 0 0 0 0
Candida parapsilosis 100 99 90 0 0 0 0 0 0 0 0 0
Candida tropicalis 100 100 100 100 0 94 99 1 0 0 1 0
Cryptococcus neoformans 1 100 82 96 9 97 50 35 1 99 100 10 0
Cryptococcus neoformans 2 100 99 47 1 1 1 5 1 98 100 52 0
Geotrichum spp *** 100 100 1 1 1 0 0 1 0 0 0 0
Saccharomyces cerevisiae 100 96 100 66 98 0 1 0 0 0 0 0
Trichosporon spp 1 99 99 99 15 80 99 95 1 70 99 99 30
Trichosporon spp 2 85 50 1 1 1 99 75 1 9 80 99 20
* Candida inconspicua / Candida norvegensis possible / möglich / posible / possibile / possível / πιθανόν / möjlig / mulig / możliwość

** Candida famata possible / möglich / posible / possibile / possível / πιθανόν / möjlig / mulig / możliwość

*** Geotrichum candidum / Geotrichum capitatum

bioMérieux SA V
api® Candida 08159I - xl - 2011/10

A noter les morphologies caractéristiques suivantes /

The following morphological characteristics should be noted /
Notieren Sie folgende morphologische Charakteristika /
Anotar las morfologías características siguientes /
Prendere nota delle seguenti caratteristiche morfologiche /
Tomar nota das seguintes morfologias características /
Τα παρακάτω μορφολογικά χαρακτηριστικά θα πρέπει να σημειωθούν /
Lägg märke till följande morfologiska kännetecken /
Bemærk følgende morfologiske karakteristika /
Należy odnotować następujące cechy morfologiczne
(Larone, 1995, Warren, 1995) :
• Chlamydospores / Chlamydosporen / Clamidosporas / Clamidospore / Clamidosporos /
Candida albicans
Χλαμυδοσπόρια / Klamydosporer / Chlamydosporer / Chlamydospory
• Petites colonies plates, blanches ou de couleur crème qui peuvent devenir roses en vieillissant /
Small, flat, white or cream-colored colonies, which may turn pink with age /
Kleine flache Kolonien, weiß oder cremefarben, ältere Kulturen zeigen ein rosa Pigment /
Pequeñas colonias planas, blancas o de color crema que pueden volverse rosas cuando envejecen /
Piccole colonie piatte, di color bianco o crema, che possono diventare rosa invecchiando /
Candida guilliermondii
Pequenas colónias achatadas, brancas ou cremes que podem virar a rosa com o passar do tempo /
Μικρές, επίπεδες, λευκού ή κρεμ χρώματος αποικίες, οι οποίες μπορεί να γίνουν ρόδινες με τον καιρό /
Små flata, vita eller krämfärgade kolonier, äldre kulturer kan vara rosa /
Små, flade, hvide eller cremefarvede kolonier, der kan blive lyserøde med alderen /
Małe, płaskie, białe lub kremowe kolonie, które mogą wraz z wiekiem stawać się różowe
• Présence de capsules (test à l'encre de Chine) / Presence of capsules (India ink test) /
Anwesenheit von Kapseln (Tuschetest) / Presencia de capsulas (test de tinta china) /
Presenza di capsule (test all'inchiostro di China) / Presença de cápsulas (teste com tinta da china) /
Παρουσία καψουλών (εξέταση India ink) / Närvaro av kapslar (Tuschfärgning) /
Tilstedeværelse af kapsler (Tuschtest) / Obecność otoczki (Test z tuszem indyjskim)
Cryptococcus neoformans
• Colonies blanches à ocre, souvent muqueuses / White to ochre colonies, often mucoid /
Weiße, ockerfarbene Kolonien, oft schleimig / Colonias de blancas a ocre, frecuentemente mucosas /
Colonie di color dal bianco all'ocra, spesso mucose / Colónias brancas a ocre, frequentemente mucosas /
Λευκές έως ωχρές αποικίες, συχνά βλεννώδεις / Vita till ockrafärgade kolonier, ofta slemmiga /
Hvide til okkerfarvede kolonier, ofte mucoide / Kolonie w kolorze białym do ochry, często śluzowe
• Mycélium vrai + arthrospores / True mycelium + arthrospores / Echtes Mycel + Arthrosporen /
Micelio + artrosporas / Vero micelio + artrospore / Micélio verdadeiro + artrosporos /
Αμιγές μυκήλιο + αρθροσπόρια / Äkta mycel + artrosporer / Ægte mycelium + artrosporer /
Prawdziwa grzybnia + artrospory
• Colonies blanches, généralement "cotonneuses" ou "duveteuses" (mycélium aérien) /
White colonies, generally "downy" or "fluffy" (aerial mycelium) /
Geotrichum spp Weiße Kolonien, im Allgemeinen "wollig" oder "flaumig" (Luftmycel) /
Colonias blancas, generalmente algodonosas o esponjosas (micelio aereo) /
Colonie bianche, generalmente "cotonose" o "lanuginose" (micelio aereo) /
Colónias brancas, geralmente algodonadas ou esponjosas (micelio aéreo) /
Λευκές αποικίες, γενικά “απαλές” ή “χνουδωτές” (αέριο μυκήλιο) /
Vita kolonier, vanligen ”luddiga” eller ”fluffiga” (luftmycel) /
Hvide kolonier, generelt "uldne" eller "dunede" /
Białe kolonie, zazwyczaj puszyste lub "pokryte meszkiem" (grzybnia powietrzna)
• Mycélium vrai + pseudomycélium + arthrospores + blastospores /
True mycelium + pseudomycelium + arthrospores + blastospores /
Echtes Mycel + Pseudomycel + Arthrosporen + Blastosporen /
Micelio + pseudomicelio + artrosporas + blastosporas /
Vero micelio + pseudomicelio + artrospore + blastospore /
Micélio verdadeiro + pseudomicélio + artrosporos + blastosporos /
Αμιγές μυκήλιο + ψευδομυκήλιο + αρθροσπόρια + βλαστοσπόρια /
Äkta mycel + pseudomycel + artrosporer + blastosporer /
Ægte mycelium + pseudomycelium + artrosporer + blastosporer /
Prawdziwa grzybnia + pseudogrzybnia + artrospory + blastospory
Trichosporon spp
• Colonies parfois ridées et adhérentes à la gélose /
Colonies occasionally wrinkled in texture which adhere to the agar /
Kolonien, manchmal gefaltet und am Agar haftend /
Colonias rugosas y adherentes al agar /
Colonie talvolta rugose ed aderenti all'agar
Colónias por vezes rugosas ou aderentes à gelose /
Αποικίες περιστασιακά ζαρωμένες στην υφή οι οποίες προσκολλώνται στο άγαρ /
Kolonier, ibland med skrynklig yta som fäster vid agarn /
Kolonier, sommetider rynkede og klæbende til agaren /
Kolonie czasami o strukturze pomarszczonej, przylegające do agaru
• Colonies roses à rouges, souvent muqueuses / Pink to red colonies, often mucoid /
Rosafarbene oder rote Kolonien, oft schleimig / Colonias de rosas a rojas, frecuentemente mucosas /
Rhodotorula spp * Colonie dal rosa al rosso, spesso mucose / Colónias rosa a vermelho, frequentemente mucosas /
Ρόδινες έως ερυθρές αποικίες, συχνά βλεννώδεις / Rosa till röda kolonier, ofta slemmiga /
Lyserøde til røde kolonier, ofte nucoide / Różowe do czewonych kolonie, często śluzowe

* absent de la base de données / not included in the database / nicht in der Datenbasis enthalten / no incluido en la base de datos /
non incluso nella base dei dati / não incluído na base de dados / δεν συμπεριλαμβάνονται στη βάση δεδομένων /
Finns inte i databasen / ikke inkluderet i databasen / nie włączony do bazy danych.

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api® Candida 08159I - xl - 2011/10

BIBLIOGRAPHIE / LITERATURE REFERENCES / LITERATUR / BIBLIOGRAFIA /

ΑΝΑΦΟΡΕΣ ΑΡΘΡΟΓΡΑΦΙΩΝ / REFERENSLITTERATUR / LITTERATURHENVISNINGER /
PIŚMIENNICTWO

1. BARNETT J.A., PAYNE R.W., YARROW D. 5. KREGER VAN RIJ N.J.W.

Yeasts : Characteristics and Identification. The Yeasts : A Taxonomic Study.
(1990) Cambridge University Press, London. (1984) Elsevier, Amsterdam.
2. BERNAL S., MARTIN MAZUELOS E., CHAVEZ M., 6. LARONE D.H.
CORONILLA J., VALVERDE A. Medically Important Fungi. A Guide to Identification.
Evaluation of the new API Candida system for identification Third Edition.
of the most clinically important yeast species. (1995) A.S.M., Washington, D.C.
(1998) Diagn. Microbiol. Infect. Dis., 32, 3, 217-221. 7. McGINNIS M.R. and al.
3. DURUSSEL C., BILLE J. Taxonomic and Nomenclatural Evaluation of the genera
API Candida, a New Simplified 12 Tests Rapid Identification Candida and Torulopsis.
System for Yeasts. (1984) J. Clin. Microbiol., 20, 813-814.
th
(1996) 96 ASM General Meeting, New Orleans, Louisiana, 8. MONGET D., DUCHESNE M.A., CANIAUX I.
F-78. api Candida, A New Identification System for Yeasts.
4. FRICKER-HIDALGO H., VANDAPEL O., DUCHESNE M.A., (1995) 7th E.C.C.M.I.D., Vienna, 26-30 March 1995.
MAZOYER M.A., MONGET D., LARDY B., LEBEAU B., 9. HOWELL S.A., HAZEN K.C.
FRENEY J., AMBROISE-THOMAS P., GRILLOT R. Candida, Cryptococcus, and Other Yeasts of Medical
Comparison of the New API Candida System to the ID 32C Importance in Manual of Clinical Microbiology.
System for the Identification of Clinically Important Yeast th
10 Edition.
Species. (2011) A.S.M., Washington, D.C., 95, 1793-1821.
(1996) J. Clin. Microbiol., 34, 1846-1848.

bioMérieux SA VII
api® Candida 08159I - xl - 2011/10

TABLE DES SYMBOLES / INDEX OF SYMBOLS / SYMBOLE / CUADRO DE SIMBOLOS /

TABELLA DEI SIMBOLI / QUADRO DOS SÍMBOLOS / ΠΙΝΑΚΑΣ ΣΥΜΒΟΛΩΝ / SYMBOLER /
SYMBOLFORTEGNELSE / TABELA SYMBOLI

Symbole / Symbol Signification / Meaning / Bedeutung

Símbolo / Simbolo Significado / Significato / Επεξήγηση
Σύμβολο Betydelse / Betydning / Znaczenie
Référence du catalogue
Catalogue number (GB) / Catalog number (US)
Bestellnummer / Número de catálogo / Numero di catalogo
Referência de catálogo / Αριθμός καταλόγου
Katalognummer / Katalognummer / Numer katalogowy
Dispositif médical de diagnostic in vitro
In Vitro Diagnostic Medical Device / In Vitro Diagnostikum
Producto sanitario para diagnóstico in vitro
Dispositivo medico-diagnostico in vitro
Dispositivo médico para diagnóstico in vitro
In Vitro Διαγνωστικό Ιατροτεχνολογικό προϊόν
Medicintekniska produkter för in vitro diagnostik
Medicinsk udstyr til in vitro-diagnostik
Wyrób do diagnostyki In Vitro
Fabricant / Manufacturer / Hersteller / Fabricante
Fabbricante / Κατασκευαστής / Tillverkare / Producent
Limites de température / Temperature limitation
Temperaturbegrenzung / Limite de temperatura
Limiti di temperatura / Limites de temperatura
Περιορισμοί θερμοκρασίας / Temperaturbegränsning
Temperaturbegrænsning / Przestrzegać zakresu temperatury
Utiliser jusque / Use by / Verwendbar bis
Fecha de caducidad / Utilizzare entro / Prazo de validade
Ημερομηνία λήξης / Använd före / Holdbar til / Użyć przed
Code du lot / Batch code / Chargenbezeichnung
Código de lote / Codice del lotto / Código do lote
Αριθμός Παρτίδας / Lot nummer / Lotnummer / Kod partii
Consulter les instructions d'utilisation
Consult Instructions for Use / Gebrauchsanweisung beachten
Consulte las instrucciones de uso
Consultare le istruzioni per l'uso
Consulte as instruções de utilização
Συμβουλευτείτε τις οδηγίες χρήσης
Se handhavandebeskrivningen / Se brugsanvisning
Sprawdź w instrukcji obsługi
Contenu suffisant pour "n" tests
Contains sufficient for <n> tests
Inhalt ausreichend für <n> Prüfungen
Contenido suficiente para <n> ensayos
Contenuto sufficiente per "n" saggi
Conteúdo suficiente para “n” ensaios
Περιεχόμενο επαρκές για «ν» εξετάσεις
Räcker till "n" antal tester
Indeholder tilstrækkeligt til "n" test
Wystarczy na wykonanie <n> testów

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