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Experiment 2_Cheek cell_Bio_Class9

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24 views

Experiment 2_Cheek cell_Bio_Class9

Uploaded by

shubhankprakash5
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Experiment No.

Aim: To prepare stained temporary mounts of human cheek cells and to record observations and
draw labelled diagram.

Theory: Cheek cell


1. The cells are flat and irregular in shape, with no cell wall.
2. The nucleus can be seen very prominently in the center of the cell.
3. The nucleus of the cell is stained easily with the methylene blue stain.
4. The cell membrane and the cytoplasm can also be studied through these cells.

Materials Required:
Slide, coverslip, watch glass, methylene blue stain, blotting paper, toothpick, needle, dropper,
brush, microscope and glycerine.

Procedure:
1. Make a dilute methylene blue solution in a watch glass.
2. Keep a clean slide with a drop of distilled water at the middle of the slide.
3. Take a clean/unused toothpick and scrap the inner wall of your mouth/cheek gently to obtain
the epithelial animal tissue, (use the blunt side of toothpick)
4. Transfer the scrap on the middle of the glass slide and put a drop of methylene blue solution
on it, to stain the cells.
5. After 2-3 minutes place the coverslip gently on the cheek cell with the help of needle and
avoid the air bubble. (A drop of glycerine can be spread on the cheek cells, it is optional)
6. With the help of blotting paper remove the extra stain/water present on the slide.
7. Place the slide under microscope and observe it.

Observations:
1. Cells with irregular shapes are seen.
2. A prominent nucleus is seen in the middle of the cell.
3. A thin membrane called plasma membrane is visible at the boundary of each cell.
4. The cells do not show any intercellular space.
5. No big vacuoles seen.
6. No cell wall seen.

(To be drawn on blank side)

Inference:
The cells observed under the microscope do not have cell wall and big vacuoles, these are the
cells of animal.

Precautions:
1. Use unused/new toothpick for scraping of cheek cells.
2. Placing of coverslip should be done carefully to avoid air bubbles.
3. Avoid overstaining.
4. Use clean/dry mounted slide while placing it under the lens of the microscope.
5. Avoid overlapping of the cells.

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