Brass petal add brass goes into solution
LAD B21 Spectrophotometry: % Cu in Brass Name ________________________________ Brooks Taylor
140 so we M
solution erlenmeyer trade
10 1 7 92 it 2
Ca is oxidized lost elections 0 28
4ᵗ
N is reduced gain electrons sᵗ
Brass is an alloy (homogenous mixture) of copper and zinc. The copper in the brass sample that will be reacted into solution
will be diluted to a particular volume, and then the absorbance of that brass solution will be measured. Cu2+ ions in solution are
blue, and the intensity of the blue solution is an indication of the concentration of the Cu2+ ions. First, we measure the
absorbance of several solutions of known concentrations of Cu2+ which we will prepare by careful dilution of a stock solution.
The absorbances of these known solutions will be measured and a graph of concentration vs. absorbance will be constructed.
Using the equation of the line from the graph of concentration of the known solution vs. absorbance of the brass sample
solution, will allows the determination of the concentration of copper ions in the brass sample solution. Knowing the
concentration of the brass sample’s volume will allow you to calculate back to the percentage of copper in the brass.
15.8 Taken notes
0.0197 mols
1g copper
u NO
63.89
Fino
0.0142mg
0.628not
58 NO
19.8V 0.628
v 0.0397 L nitric acid
LAD B1 Spectrophotometry: % Cu in Brass (p. 2 of 9)
0.0375 254 63.95
0.03252 0.25
0.596 g a
0.009375 not cut
0.009375 not cast a
0 596 9 a 1g a 2n 100 59.6 Ca
10. What would be the optimal wavelength setting to measure absorbances for the Cu2+ solutions in this lab? How do you
to
We want
know which wavelength on the colorimeter to use?
waveling
a absorbed
use M
that
motsdon
arcuate
thean
so
toes
since Cast is reflected and the
are blue we know that blue being absorbed
colors in frequireder
11. Your team will dilute a copper(II) nitrate solution to various
620 750 are being
MstockVstock = MdilutedVdiluted
concentrations. We will use the Stock Dilution Technique. Calculate
the concentration that will result from the dilutions as demonstrated,
using the formula and showing your work to the right:
Each team does 2 dilutions. Which are your team doing? Calculate Vstock based on 10 mL Vdiluted.
Team A 0.1 M solution H Team B 0.75 M sol H2o
0.1 0.9 4 6 0.1 0.4 q 1 0.75 0.9 4 6 0.75 0.4
9 I
0.2
7 3 0.1 0.7 2 8 0.1 0.2 7 3 0.7s 0.7 2 8 0.75
6 4 0.1 0.6 6 4 0.79 0.6
welsh boat
Ayaffarded 0.97 brass
2.36
LAD B1 Spectrophotometry: % Cu in Brass ( p. 3 of 9)
635 1
0.239
0.152
0.146 0.09
0.112 0.0675
0.115 0 07
0.086 0.0525
0.09 0 06
0.07 0.045
0.064
0.459
120 and rata
0.97
gold
200 150 mL
Khmu
LAD B1 Spectrophotometry: % Cu in Brass (p. 4 of 9)
Opposite in
given graph
Provided
calculations based on 8am 10M
values
1 updates graph MFate cotton to
i
V4
concentration
6 same
Concentration cutions M
2 Bress Sample 0.97 g Absorbance of solution 0.159
concentration
Concentration y 2.105 α 0.0184 y absorbance
0 159 2.105 α 0.0184 α 0.0893 u't ion concentration
0
3 Molarity Them 0.0843 m
0.0843 4ions
0.0118 moles yet
14 0.0118 moles last ions 63.546 91m Cu 0.759 cut
5 0.759 Ca 0.97g Cazzu α 100 78.231 Ca
LAD B1 Spectrophotometry: % Cu in Brass (p. 5 of 9)
This error caused oils
would have
finger to absorb or reflect additional light
from one's
skin oil on them
causing the absorbance for samples with
to be inflated and then there would be a higher
conce tration
of a tous courtus the Cu percentage to be higher
This would cause Ca the to be smaller because a
higher concentration of water would be on the surface of
the flask causing less light absorbsion due to less
concentr tion
of cast ions that absorb light
Yes since both the Ni and Ca with because ionized
the Ni ions will also absorb light caudus the experiment
resulte to be a mixture of both Ca and Ni
light absorbtion To alter the experiment to only test
for the Ni both the wavelength color of light
would need to be adjusted to optimize the NI
abortion and ideally the NI ions could be isolated
somehow
Ca ions are blue in
solution Beer's law 4 points best fit live
LAD B1 Spectrophotometry: % Cu in Brass (p. 6 of 9)
0.0027
water
0.0027 0.175L d 0004725 moles Ni't ions
A Ebc 0.728 0.350 1.0 9
bIYETTI.am 2 2.08
0.539 208 1.0 c
0.259 M solution
was smaller because there is that
on
The cuvette
relationship between b the curett width and A the absorbance
A 0.473
0.473 2 08 0.350 b
9 2.08
0.390M D 0.650 cm
LAD B1 Spectrophotometry: % Cu in Brass (p. 7 of 9)
Color and Spectrophotometry
In lab we use a colorimeter, not a spectrophotometer. Colorimeters and spectrophotometers are both instruments that measure
and analyze color, but they differ in a few ways:
• Wavelength range
Colorimeters typically operate at a fixed wavelength range of 400–700 nanometers (nm). Spectrophotometers can
measure a wider range of wavelengths, typically 190–900 nm, including ultraviolet (UV) and visible (VIS) ranges.
• Light source
Colorimeters typically use LED light sources. Spectrophotometers use light sources that can produce a range of
wavelengths.
• Filters
Colorimeters use three filters to determine the RGB values of a color. Spectrophotometers typically use 31 filters to
measure the full color spectrum.
PhET Simulation
• Go to the PhET simulation about Beer's Law (you can use the url link below or or google “PhET Beers Law”):
• http://phet.colorado.edu/sims/html/beers-law-lab/latest/beers-law-lab_en.html
• Choose the Beer's Law simulation, NOT the Concentration simulation.
• Setup: Click the red button to turn the light source on and click the Variable wavelength button to show choices in
colors of light. On the light sensor to the right, choose Absorbance (not Transmittance). Absorbance just what it
sounds like, a measure of the amount of light absorbed by the solution.
Answer the following questions below.
1. To do/notice: Click on the yellow arrow to vary the width of the solution container (the length that the light travels through
solution is called path length). Write a correlation statement comparing the amount of light absorbed as related to path
length?
The absorbance and path length are directly proportional
2. Use the blue slider at the bottom to vary the concentration of the solution. Write a correlation statement comparing the
solution concentration as related to the absorbance of light as it passes through the sample.
The absorbance and concentration are directly
porportional
3. Click the “variable” button under the light source, then slide the tab below the rainbow spectrum to change the color of light
that you are sending through the solution. For the red drink mix, what color of light absorbs the most light? What color is
least absorbed by the solution?
Green is most absorbed whereas vet absorbs the
least light
4. Change the solution to a different color solution and determine which color of light is most absorbed by each solution, and
what color of light is least absorbed. Try this for at least two different solutions.
Nick careens
msn.is Eeen is S
5. Considering the color wheel to the right, write a brief comment about what you may have noticed
from questions #3 & #4?
The most absorbent light parts are directly
across from each other on the color
wheel roughly
3.5 minutes
(p. 8 of 9)
Red
Most strongly absorb
teal green
Green
The concentrated solution has more
light absorbingions equals more light
absorbtion
None visible
Because 2n ᵗ only reflect they
absorb
do not
OrangeYellow
Purple
Purple
LAD B1 Spectrophotometry: % Cu in Brass (p. 9 of 9)
11.
Blue
12.
Red dark I
www.adf
i
msn.mn
highest possible but if
possible around 1000 am
seems to be peak absorbance
in the graphed palabora
