ANALYTICAL CHEMISTRY LAB ASSIGNMENT WORKSHEET

COURSE
CODE/COURSE

DATE OF
EXPERIMENT

GROUP NUMBER

SECTION

GROUP MEMBERS ID NO. STUDENTS’ NAME

LAB
INSTRUCTORS

ENDORSEMENT
WITH STAMP
FOR
SUBMISSION
 Faculty of Industrial Sciences & Technology
Industrial Chemistry (BPS1173)
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TABLE OF CONTENT

NO. DESCRIPTION PAGE

i. Table of content

ii. Rules in Laboratory

iii. Assignment lab work instruction

iv. Jotter

v. Rubric evaluation

vi. Main experiment instruction

vii. Question

viii. References

ix. Appendices
 Faculty of Industrial Sciences & Technology
Industrial Chemistry (BPS1173)

______________________________________________________________________

RULES IN LABORATORY
1. Students MUST wear proper attire as stated in the University’s rule.
2. For laboratory works, always put on covered shoes (sandal is not allowed). Safety shoes
must be put on in all engineering labs except in the computer lab.
3. Cap or hat is not allowed.
4. Round neck t-shirt and track bottom are not allowed. Students MUST wear Protective
(PPE) attire; lab coat, gloves and google.
5. Use or wear of jewellery is not allowed during the laboratory works.
6. Please sign your attendance.
7. Keep your books and other belongings on the racks provided outside the laboratory. Do
not place them on the laboratory bench (and do not bring library materials into the
laboratory where they might be damaged).
8. Work in the laboratory only as scheduled in the timetable unless your laboratory instructor
gives you permission.
9. Do not wander around the room/labs, distract other students, startle other students or
interfere with the laboratory experiments of others.
10. Eating and drinking are prohibited in the laboratory.
11. Report must be submitted on time (refer to lecturer/lab instructor).
12. Always follow the safety rules and any instructions given by your lab instructor.
13. Student must use proper Personal Protective Equipment (PPE) when handling equipment.
14. Report any accident (spill, breakage, etc.) or injury (cut, burn, etc.) to the instructor
immediately, no matter how trivial it might seem. Do not panic.
15. Safety First. Be alert and proceed with caution at all times in the laboratory. Notify the
instructor immediately of any unsafe conditions you observe.
16. Any broken equipment must be reported immediately. Any damages due to of
carelessness and abuse would be taken action.
17. Serious action would be taken to those who copy or duplicate the laboratory reports of
others.
18. Any previous experiments data or reports are not allowed to bring into the laboratory.
19. Completed report must be submitted and verified (stamped and signed) by staff or course
instructors.
 Faculty of Industrial Sciences & Technology
Industrial Chemistry (BPS1173)

______________________________________________________________________
20. Students are not allowed to change or reposition any laboratory equipment such
as computer, computer software, extension wire and other parts without any
permission.
21. Students must get permissions from the instructor before leaving the laboratory in
any circumstances.
22. Taking out the laboratory equipment from the lab area is prohibited.

23. No student may work in the laboratory without the presence of lab instructor.

24. Student who cannot attend the laboratory works must submit official document
with valid reason (such as medical certificate) to the instructor as soon as
possible.
25. Student who cannot attend the laboratory with valid reason may request for a
replacement for the laboratory session (depending on the available slot time of lab
instructor).
26. Make sure that all the lab tools were cleaned and put all chairs and the
experiment tools /apparatus at the right place before leaving the laboratory.
 Faculty of Industrial Sciences & Technology
Industrial Chemistry (BPS1173)

______________________________________________________________________
INSTRUCTION: PLEASE READ CAREFULLY.

ASSIGNMENT LABWORK INSTRUCTION

1. Students are required to read this lab module before entering the lab session. Jot down all
the procedure in the blank sheets in this module. It will be included in the assessment!

2. Students who are not prepared for jotter sheet are not allowed to enter the lab session.

3. Plan and organize your lab work before the lab session.

4. Students MUST complete the task on time.

5. For sample washing and drying, it requires 1 day to be completed. Hence, students
are advised to come on the next day to collect the dried sample.

6. Write all the results & discussion in this worksheet. Fill in the blanks for each
experiment.

7. Students need to prepare an executive summary in group.

8. Please submit this worksheet to the lab instructor once the experiment has been
completed.

9. Please use APA 6th format for the citation of references.

10. For appendices, students may include pictures or any relevant raw data that would
be useful for results and discussion.

11. The report could be hand-written or type-written.

12. This assignment brings 20% from the total assessment percentage in the Analytical
Chemistry course (BPS1123).
 Faculty of Industrial Sciences & Technology
Industrial Chemistry (BPS1173)
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Jot down your experiment plan down here! If you need more space, please

attach papers here!

 Faculty of Industrial Sciences & Technology
Industrial Chemistry (BPS1173)

______________________________________________________________________

RUBRIC EVALUATION FOR ENVIRONMENTAL CHEMISTRY ASSIGNMENT

LABORATORY REPORT
NO. ITEM MAX. MARKS REMARKS
MARKS OBTAINED
(%) (%)

PRACTICAL

i. Ethics 5 Attendance. PPE. Proper

attire.

ii. Team Work 10 Cooperation, willingness.

REPORT

i. Jotter experiment* 20 Follow as lab manual, lab

5 marks per sheet & extra.
experiment

ii. Discussion** 40 Explain the results &

discuss the findings.

viii. Executive summary 20 Summary of the

relationship between
parameters,
precaution & improve
method, purpose of
testing on water
samples.

ix. References/Appen 5 Result & attendance

dix sheet attached,
reference from
book/website, photos
during experiment.

TOTAL: 100

Note: To be completed by lab instructor.

 Faculty of Industrial Sciences & Technology
Industrial Chemistry (BPS1173)

______________________________________________________________________

EXP-02: BIOMARKER ANALYSIS HAIR SAMPLING – DRYING AND DISSOLVING

INTRODUCTION
Drying is the process to remove the remaining solvent in the sample by using a
laboratorial oven. The sample is further treated for the next experiment and the dried
sample is important to remove the remaining solvent which can be detected when
doing the analysis. Dissolving is a process to dissolve the solid hair sample in nitric
acid so the sample is in the solution.

OBJECTIVE
1. To complete dry the hair sample.
2. To dissolve the hair sample in an acid solution.

REAGENTS/APPARATUS

1.1 Sample tube vial

1.2 Sampling boat
1.3 Electrical balance
1.4 Beaker
1.5 Oven
1.6 Hot plate
 Faculty of Industrial Sciences & Technology
Industrial Chemistry (BPS1173)

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PROCEDURE
Hair sampling

1. Place the hair sample into a 15mL sample tube vial using a forcep
2. Cut the hair as close to the scalp as possible but be extra careful not to cut the scalp.
3. Move slightly upwards to a new location and repeat to cut the hair again.
4. Collect the hair sample in a sampling boat and weight using an electrical balance for
the weight of more than 0.20 gram.
5. After the weight of the sample hair is sufficient. Cut the hair to smaller length for
about 1-2 cm in length.
6. Place the hair sample into a 15mL sample tube vial using a forcep.
7. Fill the sampling tube vial with distilled water until the whole sample is fully
submerged.

8. Label the sampling tube vial accordingly.

9. Switch on the ultrasonic bath.
10. Press the mode to set the required time and temperature at the panel of the
ultrasonic bath. Heater is set to be off. Sonification strength is control by turning the knob
to be at the middle.

11. Place the sampling tube vial in the rack and make sure the level of bath water is
above the hair sample.
12. Remove the water in the sampling tube vial using a vacuum filtration apparatus. Use
a clipper or clamp to fasten the upper part of the glassware. Switch on the main switch at
the wall socket and the switch at the pump. Pour the sample into the filter and wait until
no drop can be seen.

13. Switch off the vacuum pump. Remove the upper clamp and carefully remove the
upper glassware of the filtration part.
QUESTIONS
1. Explain the purpose of using both distilled water and acetone for washing ?

2. Explain the purpose of using an electronic bath in the washing method ?

3. Explain why students are required to use gloves or forceps which prevent the direct
bare-hand contact with the hair sample ?